Acting On Alpha-1, 4-glucosidic Bond, (e.g., Hyaluronidase, Invertase, Amylase, Etc. (some 3.2.1)) Patents (Class 435/201)
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Patent number: 8273348Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, an are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.Type: GrantFiled: December 12, 2007Date of Patent: September 25, 2012Assignees: Novozymes A/S, Novozymes Inc.Inventors: Allan Svendsen, Michael Skjoet, Debbie Yaver, Lars Lehmann Hylling Christensen, Signe Eskildsen Larsen, Nina Lundin, Michael Lamsa, Peter Colin Gregory
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Patent number: 8269061Abstract: Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.Type: GrantFiled: October 3, 2011Date of Patent: September 18, 2012Assignee: E.I. du Pont de Nmeours and CompanyInventor: Mark E. Williams
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Publication number: 20120231504Abstract: A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation.Type: ApplicationFiled: September 3, 2011Publication date: September 13, 2012Applicant: Therapeutic Proteins Inc.Inventor: Sarfaraz K. Niazi
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Patent number: 8263368Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: GrantFiled: June 29, 2009Date of Patent: September 11, 2012Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Patent number: 8263381Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: GrantFiled: August 20, 2010Date of Patent: September 11, 2012Assignees: Novozyms A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielsen, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Publication number: 20120213767Abstract: Soluble PH20 polypeptides are provided, including extended soluble PH20 polypeptides, and uses thereof. Also provided are other C-terminally truncated PH20 polypeptides and partially deglycosylated PH20 polypeptides and uses thereof.Type: ApplicationFiled: February 21, 2012Publication date: August 23, 2012Inventors: Ge Wei, Krishnasamy Panneer Selvam, Louis Bookbinder, Gregory I. Frost
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Publication number: 20120214197Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: August 23, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Patent number: 8247210Abstract: Improved host cells and culture methods involving overexpression of MAN1C1 activity to improve protein production are provided.Type: GrantFiled: May 21, 2010Date of Patent: August 21, 2012Assignee: Amgen Inc.Inventor: Christopher K. Crowell
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Publication number: 20120208251Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.Type: ApplicationFiled: April 25, 2012Publication date: August 16, 2012Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Publication number: 20120196348Abstract: Provided are methods for preparing culture medium that contains soluble hyaluronidases. The methods employ cells that contain a plurality of active copies of nucleic acid encoding the soluble hyaluronidase and a plurality of feedings and temperature changes, whereby the encoded soluble hyaluronidase is secreted into the cell culture medium.Type: ApplicationFiled: March 13, 2012Publication date: August 2, 2012Inventors: David Baker, Louis H. Bookbinder
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Publication number: 20120190075Abstract: This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.Type: ApplicationFiled: November 2, 2011Publication date: July 26, 2012Inventors: Karsten Matthias Kragh, Anja Hemmingsen Kellet-Smith, René Mikkelsen, Rie Mejldal, Rikke L. Bundgaard Jenner
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Patent number: 8227667Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 27, 2012Date of Patent: July 24, 2012Assignee: Novozymes A/SInventors: Suchindra Maiyuran, Paul Harris, Hanshu Ding, Kimberly Brown
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Publication number: 20120183995Abstract: The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been inactivated, and preferably wherein one or more chromosomal genes have been deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been deleted from a corresponding wild-type Bacillus host chromosome.Type: ApplicationFiled: January 23, 2012Publication date: July 19, 2012Applicant: Danisco US Inc.Inventors: Eugenio Ferrari, Carole Harbison, M. Harunur Rashid, Walter Weyler
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Patent number: 8222035Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.Type: GrantFiled: June 24, 2010Date of Patent: July 17, 2012Assignee: Verenium CorporationInventors: Walter Callen, Toby Richardson, Gerhard Frey, Kevin A. Gray, Janne S. Kerovuo, Malgorzata Slupska, Nelson R. Barton, Eileen O'Donoghue, Carl Miller
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Publication number: 20120171722Abstract: The invention provides a method for producing polypeptide protein products and nucleic acid products having reduced levels of antigenicity in an animal being treated with a biologic product. Somatic cells are isolated from an animal, transformed into pluripotent stem cells, transfected with a nucleic acid(s) of interest, and re-differentiated towards somatic cells known to be high level producers of the desired nucleic acid product. The invention can be used to derive a general cell line to treat populations, racial specific cell lines to treat ethnic groups, or patient specific cell lines to treat individuals. Additionally, the invention provides a method to allow induced pluripotent stem cells to be re-differentiated towards their somatic cell of origin so that the cells can be used to therapeutically treat an animal without resulting teratoma formation.Type: ApplicationFiled: January 3, 2012Publication date: July 5, 2012Applicant: AVM Biotechnology, LLCInventor: Theresa A. Deisher
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Publication number: 20120171748Abstract: Compositions and methods relating to an alpha-amylase enzyme obtained from Halomonas variabilis WDG195 are described.Type: ApplicationFiled: February 25, 2010Publication date: July 5, 2012Inventors: Melodie Estabrook, Brian E. Jones, Marc Kolkman, Chris Leeflang, Leo P.M. Van Marrewijk
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Patent number: 8211665Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: September 29, 2010Date of Patent: July 3, 2012Assignees: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Hanshu Ding
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Publication number: 20120164127Abstract: This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at the wild type normal physiological conditions. For example, evolved proteins are virtually inactive at body temperature, but are active at lower temperatures.Type: ApplicationFiled: March 9, 2010Publication date: June 28, 2012Inventors: Jay M. Short, Hwai Wen Chang, Gerhard Frey, Gregory Frost
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Patent number: 8206966Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.Type: GrantFiled: November 3, 2008Date of Patent: June 26, 2012Assignee: Danisco US Inc.Inventors: Luis G. Cascāo-Pereira, James T. Kellis, Jr., Bradley A. Paulson, Scott D. Power, Sandra W. Ramer, Vivek Sharma, Andrew Shaw, Jayarama K. Shetty, Donald E. Ward
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Patent number: 8202517Abstract: The invention relates to the discovery of novel soluble neutral active Hyaluronidase Glycoproteins (sHASEGP's), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. The invention further comprises sialated and pegylated forms of a recombinant sHASEGP to enhance stability and serum pharmacokinetics over naturally occurring slaughterhouse enzymes. Further described are suitable formulations of a substantially purified recombinant sHASEGP glycoprotein derived from a eukaryotic cell that generate the proper glycosylation required for its optimal activity.Type: GrantFiled: February 20, 2009Date of Patent: June 19, 2012Assignee: Halozyme, Inc.Inventors: Louis H. Bookbinder, Anirban Kundu, Gregory I. Frost
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Publication number: 20120142047Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.Type: ApplicationFiled: November 29, 2011Publication date: June 7, 2012Applicant: Therapeutic Proteins, Inc.Inventor: Sarfaraz K. Niazi
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Patent number: 8187855Abstract: Provided are methods for preparing large-scale preparations of soluble hyaluronidases. The methods employ cells that contain a plurality of active copies of nucleic acid encoding the soluble hyaluronidase and a plurality of feedings and temperature changes, whereby the encoded soluble hyaluronidase is secreted into the cell culture medium.Type: GrantFiled: March 6, 2009Date of Patent: May 29, 2012Assignee: Halozyme, Inc.Inventors: David Baker, Louis H. Bookbinder
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Publication number: 20120129218Abstract: The present invention relates to a host cell comprising a cofilin-specific small interfering RNA (siRNA) sequence. The host cell may further comprise a nucleic acid encoding a recombinant protein. The present invention also relates to a method for producing a recombinant protein by the host cell comprising a cofilin-specific small interfering RNA (siRNA) sequence.Type: ApplicationFiled: November 11, 2011Publication date: May 24, 2012Applicant: UNIVERSITY OF DELAWAREInventors: KELVIN H. LEE, STEPHANIE HAMMOND
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Patent number: 8183029Abstract: Refractile particles containing a heterologous polypeptide as an insoluble aggregate are recovered from bacterial periplasm. The process involves culturing bacterial cells so as to express nucleic acid encoding phage lysozyme and nucleic acid encoding the heterologous polypeptide under separate promoters, disrupting the cells mechanically to release the phage lysozyme so as to release refractile particles from the bacterial cellular matrix, and recovering the released refractile particles from the periplasm. Chloroform is not used in any step and the recovery step minimizes co-recovery of cellular debris with the released refractile particles.Type: GrantFiled: March 19, 2010Date of Patent: May 22, 2012Assignee: Genentech, Inc.Inventors: Woon-Lam Susan Leung, James R. Swartz
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Publication number: 20120122110Abstract: The present invention relates to a method and kit for the isolation of cells from a sample. The sample is treated with an extraction solution that comprises at least MgCl2 and/or an ionic liquid resulting in the isolation of preferably viable cells.Type: ApplicationFiled: May 31, 2010Publication date: May 17, 2012Applicant: MERCK PATENT GESELLSCHAFT MIT BESDCHRANKTER HAFTUNGInventors: Peter Rossmanith, Patrick Julian Mester, Martin Wagner
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Patent number: 8178336Abstract: We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).Type: GrantFiled: December 19, 2008Date of Patent: May 15, 2012Assignee: Danisco A/SInventors: Patrick Maria Franciscus Derkx, Anja Kellet-Smith Hemmingen, Rie Mejldal, Bo Spange Sørensen, Karsten Matthias Kragh
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Publication number: 20120115186Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.Type: ApplicationFiled: July 6, 2010Publication date: May 10, 2012Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
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Publication number: 20120107905Abstract: The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants.Type: ApplicationFiled: October 28, 2011Publication date: May 3, 2012Applicant: AB ENZYMES OYInventors: Kari Juntunen, Leena Valtakari, Nina Hakulinen, Marja Paloheimo
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Publication number: 20120094358Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.Type: ApplicationFiled: December 6, 2011Publication date: April 19, 2012Applicant: XYLECO, INC.Inventor: Marshall Medoff
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Publication number: 20120094937Abstract: Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.Type: ApplicationFiled: December 20, 2011Publication date: April 19, 2012Applicant: Schering CorporationInventors: Daniel M. Gorman, Troy D. Randall, Albert Zlotnik
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Publication number: 20120090054Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Paul Harris, Elena Valsenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen
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Patent number: 8153412Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.Type: GrantFiled: November 3, 2008Date of Patent: April 10, 2012Assignee: Danisco US Inc.Inventors: Claudine Y. Chang, Clement Choy, Melodie Estabrook, Mansi Goyal, Thomas P. Graycar, Victoria E. Huang, Brian E. Jones, Marc Kolkman, Karsten M. Kragh, Chris Leeflang, Scott D. Power, Sandra W. Ramer, Andrew Shaw, Casper Vroemen, Walter Weyler
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Patent number: 8143038Abstract: A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene.Type: GrantFiled: July 26, 2011Date of Patent: March 27, 2012Assignee: TMO Renewables LimitedInventors: Anthony Atkinson, Roger Cripps, Kirstin Eley, Brian Rudd, Martin Todd
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Patent number: 8137944Abstract: Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.Type: GrantFiled: July 7, 2005Date of Patent: March 20, 2012Assignee: Danisco A/SInventors: Casper Tune Berg, Patrick M. F. Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sørensen, Charlotte Refdahl Thoudahl
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Publication number: 20120064601Abstract: Described herein are composite materials and methods of using them for the separation or purification of enantiomers. In certain embodiments, the composite material comprises a support member, comprising a plurality of pores extending through the support member; and a macroporous cross-linked gel, comprising a plurality of macropores, and a plurality of pendant chiral moieties. In certain embodiments, the composite materials may be used in the separation or purification of a chiral small molecule.Type: ApplicationFiled: September 13, 2011Publication date: March 15, 2012Inventors: Elena N. Komkova, Amro Ragheb, Charles H. Honeyman
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Patent number: 8124394Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: September 26, 2008Date of Patent: February 28, 2012Assignee: Novozymes, A/SInventors: Suchindra Maiyuran, Paul Harris, Hanshu Ding
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Publication number: 20120045817Abstract: Described are compositions and methods relating to variant alpha-amylases having altered biochemical properties and advantageous performance characteristics as compared to a reference alpha-amylase. The variants are suitable for use in various industrial applications such as starch conversion, ethanol production, laundry, dishwashing, pulp and paper production, textile desizing, and/or sweetener production.Type: ApplicationFiled: April 1, 2010Publication date: February 23, 2012Inventors: David A. Estell, Brian E. Jones, Marc Kolkman, Christian D. Adams, Edward M. Concar
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Publication number: 20120045816Abstract: Provided herein are glycosylated polypeptide compositions with substantially reduced Neu5Gc content. The glycosylated polypeptides compositions with substantially reduced Neu5Gc content can be obtained from cell sources cultured with Neu5Gc competitor or from non-human animal sources fed a diet supplemented with Neu5Gc competitor. Also provided herein are methods of treating a human subject with said compositions.Type: ApplicationFiled: July 14, 2011Publication date: February 23, 2012Inventors: Darius Ghaderi, Ajit Varki
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Patent number: 8119384Abstract: The present invention relates to processes for producing a starch hydrolyzate and optionally a fermentation product, such as ethanol.Type: GrantFiled: June 15, 2007Date of Patent: February 21, 2012Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Anders Viksoe-Nielsen, Jim Liu, Carsten Andersen
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Publication number: 20120034342Abstract: The present invention relates to a method for obtaining a preparation of ?-amylases from the soluble fraction of starch plants, characterized in that the soluble fraction of starch plants is selected from the group consisting of the soluble fractions of wheat, pea, broad bean, horse bean, rice, barley, rye, buckwheat, potato and sweet potato, and preferably of wheat and barley, a clarification of said soluble fractions is carried out in such a way as to remove therefrom the insoluble substances and the colloids and, optionally, an ultrafiltration of said clarified soluble fractions is carried out in such a way as to obtain an ultrafiltration retentate containing the concentrated ?-amylase and an ultrafiltration permeate, said ultrafiltration retentate containing the concentrated ?-amylase is diafiltered and the resulting ?-amylase is recovered.Type: ApplicationFiled: March 30, 2010Publication date: February 9, 2012Applicant: ROQUETTE FRERESInventors: Vincent Courbois, Pierrick Duflot, Damien Passe
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Patent number: 8110380Abstract: A starch-based biodegradable material composition includes: an enzyme-hydrolyzed starch; and a biodegradable polyester selected from at least one of an aliphatic polyester of polybutylene succinate and an aliphatic-aromatic copolyester. The enzyme-hydrolyzed starch is prepared by hydrolyzing a native starch using a starch-hydrolyzing enzyme. The starch-hydrolyzing enzyme has an activity unit ranging from 15000 to 40000.Type: GrantFiled: April 29, 2008Date of Patent: February 7, 2012Assignee: Grace Biotech CorporationInventors: C. Will Chen, Ching-Huang Wang, Chin-Wei Chen, Yun-Ping Wang
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Patent number: 8101392Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.Type: GrantFiled: October 15, 2010Date of Patent: January 24, 2012Assignee: Novozymes A/SInventors: Bjarne Roenfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Publication number: 20120009594Abstract: Disclosed are a method for measuring ?G comprising the steps of bringing a sample into contact with a ?G-binding protein 1 and a ?G-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID NO:20, and having the ?-glucan binding activity, to form a complex of the ?G-binding protein 1, ?G in the sample and the ?G-binding protein 2, measuring quantity of the complex, and determining ?G concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel ?G-binding protein; a nucleic acid molecule encoding the ?G-binding protein; and a method for producing the aforementioned ?G-binding protein.Type: ApplicationFiled: March 17, 2010Publication date: January 12, 2012Applicant: WAKO PURE CHEMICAL INDUSTRIES, LTD.Inventor: Akito Yoneda
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Patent number: 8093023Abstract: An improved process for producing de-fatted soy utilizing a de-fatted soy flour and for producing value added by-products from de-fatted soy flour wherein soybeans are de-hulled and the de-hulled stream ground to a flour consistency. The ground soy flour is mixed with water and other additives to produce a vitamin and mineral enriched stream that is then filtered to various value added by-products. In a preferred embodiment the vitamin and mineral enriched stream is filtered through a 0.1-1.0 micron membrane to produce a de-fatted soy product stream and a fatted soy product stream. The fatted soy product stream can be dried to produce dry, less than 12% water by weight, product for use in cosmetics and pharmaceutical products. The de-fatted soy product can be filtered through reverse osmosis (RO) filtration unit to obtain a vitamin and mineral enriched product stream that can be dried to powder form and used as a food supplement additive.Type: GrantFiled: September 28, 2005Date of Patent: January 10, 2012Assignee: Little Sioux Corn Processor, LLC.Inventors: John E. Prevost, Neal A. Hammond
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Publication number: 20120003715Abstract: A method for expressing and industrial scale production of recombinant lysosomal enzymes utilizing insect larva including the steps of infecting an insect larva population of the species Spodoptera littoralis via a recombinant baculovirus liquid suspension which permits the expression of at least one gene coding for a protein of interest, at least one of these genes coding for a lysosomal enzyme expressed in the insect larva, collecting of the previously infected insect larva expressing in a sufficient significant quantity the protein of interest and recovering the protein of interest from the collected insect larva.Type: ApplicationFiled: March 13, 2009Publication date: January 5, 2012Applicant: Bioorganic Research and Services S.L.Inventors: Victor Infante Viñolo, Rosa Maria Osuna Aguilar, Juan Jose Infante Viñolo
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Publication number: 20120003700Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.Type: ApplicationFiled: September 13, 2011Publication date: January 5, 2012Applicant: Novozymes A/SInventors: Tang Lan, Wu Wenping, Junxin Duan, Pia Francke Johannesen
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Patent number: 8084025Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.Type: GrantFiled: April 20, 2009Date of Patent: December 27, 2011Assignee: Curemark LLCInventor: Joan M. Fallon
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Patent number: 8080401Abstract: The present invention relates to ?-amylase variants that are stabilized to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, by point mutagenesis of asparagine (N) or glutamine (Q) residues located on the surface of the molecule to give other amino acid residues. The invention further relates to methods of increasing the stability of an ?-amylase to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, whereby at least one asparagine (N) or glutamine (Q) residue on the surface of the molecule is replaced with a different amino acid residue. The ?-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.Type: GrantFiled: March 28, 2007Date of Patent: December 20, 2011Assignee: Henkel AG & Co. KGaAInventors: Cornelius Bessler, Susanne Wieland, Karl-Heinz Maurer
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Publication number: 20110300201Abstract: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.Type: ApplicationFiled: November 3, 2009Publication date: December 8, 2011Applicant: DANISCO US INC.Inventors: Nathaniel T. Becker, Michael Stoner, Mee-Young Yoon
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Publication number: 20110300585Abstract: The various embodiments of the present invention relate generally to compositions, systems, and methods for altering rates of catalysis. More particularly, the various embodiments of the present invention are directed toward compositions, systems, and methods for enzymatic hydrolysis of polysaccharides, such as cellulose and starch. An aspect of the present invention comprises a method for altering the rate of conversion of a substrate into a product comprising: providing a substrate in a carrier; mixing a reactant and a co-factor with the carrier to form a substantially homogeneous mixture of the reactant, the co-factor, and the substrate in the carrier; and reacting the reactant with the substrate in the presence of the co- factor to convert at least a portion of the substrate into the product, wherein the reaction rate of the reactant with the substrate in the presence of the co-factor is different than the reaction rate of the reactant with the substrate in the absence of the co-factor.Type: ApplicationFiled: December 18, 2008Publication date: December 8, 2011Applicant: Georgia Tech Research CorporationInventors: Sujit Banerjee, John T. Reye