Aspergillus Patents (Class 435/254.3)
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Publication number: 20140073019Abstract: The present invention relates to the field of biotransformation of furanic compounds. More particular the present invention relates to novel genetically modified cells with improved characteristics for biocatalytic transformation of furanic compounds and a vector suitable for the genetic modification of a host cell. Further aspects of the invention are aimed at processes for biotransformation of 5-(hydroxymethyl)furan-2-carboxylic acid (HMF-acid) and its precursors with the use of the cell according to the invention.Type: ApplicationFiled: March 7, 2012Publication date: March 13, 2014Applicant: PURAC BIOCHEM B.V.Inventors: Nick Johannes Petrus Werckx, Tom Daniel Elink Schuurman, Sipko Maarten Kuijper, Harald Johan Ruijssenaars
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Publication number: 20140072980Abstract: A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has an immunoglobulin CH3 domain and/or substituted CH3 domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.Type: ApplicationFiled: November 14, 2013Publication date: March 13, 2014Applicant: Regeneron Pharmaceuticals, Inc.Inventors: JAMES P. FANDL, GANG CHEN, NEIL STAHL, GEORGE D. YANCOPOULOS, DIPALI DESHPANDE, DARYA BURAKOV, THOMAS ALDRICH, VISHAL KAMAT
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Publication number: 20140075601Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: October 28, 2013Publication date: March 13, 2014Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20140065678Abstract: The invention relates to recombinant expression of a variant form of a fungal C1 strain ?-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the ?-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant ?-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.Type: ApplicationFiled: November 8, 2013Publication date: March 6, 2014Applicant: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Attila Andor
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Publication number: 20140065671Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Mary Ann Stringer, Brett McBrayer
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Publication number: 20140065677Abstract: The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicant: Novozymes A/SInventors: Lene Lange, Peter K. Busk
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Publication number: 20140065693Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.Type: ApplicationFiled: October 9, 2013Publication date: March 6, 2014Applicant: ROAL OYInventors: Terhi Puranen, Sauli Toikka, Kim Langfelder, Jari Vehmaanpera
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Publication number: 20140059719Abstract: The present invention relates to chimeric GH61 polypeptides having cellulolytic enhancing activity. The present invention also relates to polynucleotides encoding the chimeric GH61 polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the chimeric GH61 polypeptides.Type: ApplicationFiled: November 18, 2011Publication date: February 27, 2014Applicant: Novozymes, Inc.Inventors: Mark Wogulis, Matthew Sweeney, Tia Heu
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Publication number: 20140051146Abstract: In one aspect there is provided a method for producing a recombinant enzyme capable of hydrolysing chlorophyll or a chlorophyll derivative, comprising a step of intracellular expression of the recombinant enzyme in a eukaryotic host cell.Type: ApplicationFiled: February 16, 2012Publication date: February 20, 2014Applicant: DUPONT NUTRITION BIOSCIENCES APSInventor: Susan Mampusti Madrid
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Publication number: 20140051119Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: June 10, 2013Publication date: February 20, 2014Applicant: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20140051130Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: October 11, 2013Publication date: February 20, 2014Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Publication number: 20140045215Abstract: The present invention relates to novel humanized, chimeric and murine antibodies that have binding specificity for the human CC chemokine ligand 20 (CCL20). The present invention further relates to heavy chains and light chains of said antibodies. The invention also relates to isolated nucleic acids, recombinant vectors and host cells that comprise a sequence which encodes a heavy chain and/or a light chain of said antibodies, and to a method of preparing said antibodies. The anti-CCL20 antibodies of the invention can be used in therapeutic applications to treat, for example, inflammatory and autoimmune disorders and cancer.Type: ApplicationFiled: June 21, 2013Publication date: February 13, 2014Inventors: Toshio Imai, Brad Kline, Tetsu Kawano, Luigi Grasso, Yoshimasa Sakamoto, Jared Spidel, Miyuki Nishimura, Kenzo Muramoto, Tatsuo Horizoe
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Publication number: 20140045230Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.Type: ApplicationFiled: October 21, 2013Publication date: February 13, 2014Applicant: DSM IP ASSETS B.V.Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
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Publication number: 20140044835Abstract: The invention relates to a synthetic phytase with elevated thermostability, elevated stability to acids at p H 2, elevated stability to pepsin and with a broadened active p H range, and to an isolated nucleic acid sequence coding for a synthetic phytase and to the use of the phytase in an animal feed for reducing the phosphate content in the slurry and to animal feed additives and animal feeds comprising the synthetic phytase.Type: ApplicationFiled: April 18, 2012Publication date: February 13, 2014Applicant: BASF SEInventors: Stefan Haefner, Annegret Welzel, Robert Thummer
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Patent number: 8648183Abstract: It is intended to provide base sequences participating in the regulation of the expression of a promoter. It is also intended to modify a promoter based on the base sequence data to give a modified promoter having a high expression activity. Namely, a modified promoter constructed by inserting a first DNA fragment containing CCAATNNNNNN (SEQ ID NO:1) (a first base sequence) and a second DNA fragment containing CGGNNNNNNNNNGG (SEQ ID NO:2) (a second base sequence) into a promoter functioning in a filamentous fungus, wherein N in the base sequence denotes A (adenine), G (guanine), C(cytosine), or T (thymine).Type: GrantFiled: February 28, 2003Date of Patent: February 11, 2014Assignee: Amano Enzyme Inc.Inventors: Kanako Suzuki, Norihiro Tsukagoshi
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Patent number: 8642298Abstract: The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in microorganisms.Type: GrantFiled: March 4, 2009Date of Patent: February 4, 2014Assignee: Nederlandse Organisatie Voor Toegepast—Natuurwetenschappelijk Onderzoek TnoInventors: Maria Johanna Van Der Werf, Peter Jan Punt
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Publication number: 20140031587Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.Type: ApplicationFiled: October 2, 2013Publication date: January 30, 2014Applicant: DSM IP Assets B.V.Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
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Publication number: 20140030770Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: January 30, 2014Applicant: NOVOZYMES INC.Inventor: Nikolaj Spodsberg
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MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS
Publication number: 20140030779Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.Type: ApplicationFiled: June 3, 2013Publication date: January 30, 2014Applicant: GENOMATICA, INC.Inventors: Priti PHARKYA, Anthony P. BURGARD, Stephen J. VAN DIEN, Robin E. OSTERHOUT, Mark J. BURK, John D. TRAWICK, Michael P. KUCHINSKAS, Brian STEER -
Publication number: 20140033373Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.Type: ApplicationFiled: June 23, 2011Publication date: January 30, 2014Applicant: DSM IP ASSETS B.V.Inventors: Margot Elisabeth Francoise Schooneveld-Bergmans, Wilbert Herman Marie Heijne, Monica Diana Vlasie, Robbertus Antonius Damveld
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Publication number: 20140033369Abstract: Provided is a polynucleotide encoding a protein having an activity to transfer a sugar to the hydroxy groups at the 4?- and 7-positions of a flavone.Type: ApplicationFiled: January 11, 2012Publication date: January 30, 2014Applicant: SUNTORY HOLDINGS LIMITEDInventors: Yoshikazu Tanaka, Naoko Okitsu, Keisuke Matsui
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Patent number: 8637101Abstract: The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well as functional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.Type: GrantFiled: February 26, 2009Date of Patent: January 28, 2014Assignee: DSM IP Assets B.V.Inventors: Jan Metske Laan Van Der, Yulia M. Efimova, Karin Turk, Margot Elisabeth Francoise Schooneveld-Bergmans, Arie Gerrit Terdu, Arjen Sein, Natalja Alekseevna Cyplenkova
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Publication number: 20140024862Abstract: A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.Type: ApplicationFiled: January 2, 2013Publication date: January 23, 2014Inventors: Michael Katz, Jochen Forster, Helga David, Hans Peter Schmidt, Malin Sendelius, Sara Peterson Bjorn, Thomas Thomasen Durhuus
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Publication number: 20140023644Abstract: The present disclosure relates, in general, to materials and methods for antibodies specific for transforming growth factor beta (TGF?), including TGF?1, TGF?2 and TGF?3, and uses of these antibodies in the treatment of subjects having cancer, an eye disease, condition or disorder, fibrosis, including ophthalmic fibrosis or fibrosis of the eye, and other conditions or disorders related to TGF? expression.Type: ApplicationFiled: September 26, 2013Publication date: January 23, 2014Applicant: XOMA Technology Ltd.Inventors: Daniel Bedinger, Shireen S. Khan, Amer Mirza, Ajay J. Narasimha, Toshihiko Takeuchi
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Publication number: 20140026261Abstract: The present invention provides isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: April 25, 2012Publication date: January 23, 2014Inventors: Yu Zhang, Lan Tang, Svend Hostgaard Bang Henriksen
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Publication number: 20140024086Abstract: The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.Type: ApplicationFiled: March 29, 2012Publication date: January 23, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Elliot N. Miller, Xueli Zhang, Lorraine P. Yomano, Xuan Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Patent number: 8633010Abstract: The present invention relates to an isolated polypeptide having PepC inhibitory activity as well as to a method for producing a heterologous polypeptide of interest in an Aspergillus host cell comprising: (a) cultivating the Aspergillus host cell comprising a first and a second nucleic acid sequences under conditions conducive for the expression of the polypeptides encoded by the said first and second nucleic acid sequences, and wherein the first nucleic acid sequence encodes a heterologous polypeptide of interest and the second nucleic acid encodes the inhibitor polypeptide of the invention, and wherein the inhibitor polypeptide is expressed from a recombinant nucleic acid construct resulting in an increased level of the inhibitor polypeptide compared to an Aspergillus host cell not comprising the recombinant nucleic acid construct; and (b) recovering the heterologous polypeptide.Type: GrantFiled: December 2, 2008Date of Patent: January 21, 2014Assignee: Novozymes A/SInventors: Jan Lehmbeck, Hiroaki Udagawa
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Publication number: 20140017733Abstract: The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.Type: ApplicationFiled: June 10, 2013Publication date: January 16, 2014Inventors: Nicholas John Agard, David Elgart, Jie Yang, Goutami Banerjee, Jeanne Bonomo Benoit, Dipnath Baidyaroy
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Publication number: 20140018283Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: ApplicationFiled: September 24, 2013Publication date: January 16, 2014Applicant: Novozymes A/SInventors: Tina Sejersgard Fano, Claus Von Der Osten, Malene Kappen Kruger, Mads Norregaard-Madsen
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Publication number: 20140017737Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 23, 2013Publication date: January 16, 2014Inventor: Mark Wogulis
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Publication number: 20140017695Abstract: A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has a T cell receptor variable domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.Type: ApplicationFiled: January 10, 2013Publication date: January 16, 2014Applicant: REGENERON PHARMACEUTICALS, INC.Inventor: REGENERON PHARMACEUTICALS, INC.
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Publication number: 20140011236Abstract: The present invention provides, in part, promoters for recombinant expression of polypeptides in host cells such as Pichia pastoris as well as methods of use thereof.Type: ApplicationFiled: March 15, 2012Publication date: January 9, 2014Inventors: Robert Davidson, Bianka Prinz, I-Ming Wang, Stephen Hamilton, Ming-Tang Chen, Brian Mickus
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Publication number: 20140011262Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3? end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.Type: ApplicationFiled: July 18, 2013Publication date: January 9, 2014Applicant: NOVOZYMES, INC.Inventors: Suchindra Maiyuran, Ana Fidantsef, Howard Brody
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Publication number: 20140007301Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: January 2, 2014Applicants: NOVOZYMES A/S, NOVOZYMES NORTH AMERICA, INCInventors: Marc D. Morant, Mikako Sasa, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20140004526Abstract: The invention relates to suitable screening strategies for evaluating various candidate promoters for differential gene expression during the propagation and production phases of a fermentation process. The invention also relates to recombinant host cells that comprise identified promoter nucleic acid sequences and methods for producing fermentation products employing the same.Type: ApplicationFiled: December 28, 2012Publication date: January 2, 2014Applicant: Butamax™ Advanced Biofuels LLCInventor: Butamax™ Advanced Biofuels LLC
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Patent number: 8617859Abstract: The present invention relates to methods of producing C4 dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4 dicarboxylic acid transporter; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, as well as host cells comprising the polynucleotides.Type: GrantFiled: June 2, 2011Date of Patent: December 31, 2013Assignee: Novozymes, Inc.Inventors: Sarah McFarland, Amanda Fischer
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Publication number: 20130344573Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: ApplicationFiled: August 16, 2013Publication date: December 26, 2013Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Publication number: 20130344541Abstract: The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures.Type: ApplicationFiled: November 1, 2011Publication date: December 26, 2013Applicant: CODEXIS, INC.Inventors: Ish Kumar Dhawan, Dipnath Baidyaroy, Andrew Shaw, Oleh Tanchak, Christopher Hill, Chengsong Liu, Amala Chokshi, Brian R. Scott
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Publication number: 20130344076Abstract: The present application provides the amino acid and nucleic acid sequences of heavy and light chain complementarity determining regions of a cancer specific antibody directed to an epitope of variant Nuclear Factor Kappa-B inhibitor beta (NFKBIB). In addition, the application provides cancer specific antibodies and immunoconjugates comprising the cancer specific antibody attached to a toxin or a label, and methods of uses thereof. The application also relates to diagnostic methods and kits using the cancer specific antibodies herein. Further, the application provides novel cancer-associated epitopes and antigens of variant NFKBIB, and uses thereof.Type: ApplicationFiled: March 26, 2013Publication date: December 26, 2013Applicant: Viventia Bio Inc.Inventors: Francina C. CHAHAL, Jeannick CIZEAU
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Publication number: 20130340103Abstract: The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.Type: ApplicationFiled: June 6, 2013Publication date: December 19, 2013Applicant: BASF Plant Science GmbHInventors: Jörg Bauer, Tom Wetjen
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Publication number: 20130333072Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: December 12, 2013Applicants: Novozymes North America, Inc., Novozymes A/SInventors: Sara Landvik, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20130330780Abstract: The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed.Type: ApplicationFiled: November 24, 2010Publication date: December 12, 2013Inventors: Jari Natunen, Anne Kanerva, Jukka Hiltunen, Markku Saloheimo, Heli Viskari, Anne Huuskonen
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Publication number: 20130330784Abstract: The invention relates to temperature-stable polypeptides with ?-pyranosidase activity. The polypeptide substrates include ?-glucopyranosides und ?-xylopyranosides. Said polypeptides can be expressed alone or as fusion proteins for example in yeast or bacteria and subsequently purified. The polypeptides according to said invention can be used alone or in a mixture with other enzymes for the degradation of plant raw materials, among others for the enzymatic degradation of biomass containing lignocellulose, in particular hemicellulose and the hemicellulose component xylan. Said enzymes are suitable for use in textile processing, as an additive of detergents, or in the food or feed industry.Type: ApplicationFiled: October 26, 2011Publication date: December 12, 2013Applicant: CLARIANT PRODUKTE (DEUTSCHLAND) GMBHInventors: Christoph Reisinger, Farah Qoura, Barbara Klippel, Garabed Antranikian
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Publication number: 20130316409Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: November 12, 2012Publication date: November 28, 2013Applicant: VERENIUM CORPORATIONInventor: VERENIUM CORPORATION
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Publication number: 20130315918Abstract: Anti-VEGF antibodies and variants thereof, including those having high affinity for binding to VEGF, are disclosed. Also provided are methods of using phage display technology with naïve libraries to generate and select the anti-VEGF antibodies with desired binding and other biological activities. Further contemplated are uses of the antibodies in research, diagnostic and therapeutic applications.Type: ApplicationFiled: June 27, 2013Publication date: November 28, 2013Applicant: Genentech, Inc.Inventors: GERMAINE FUH, HANS-PETER GERBER, WEI-CHING LIANG, FREDERIC FELLOUSE, SACHDEV S. SIDHU, CHRISTIAN WIESMANN
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Publication number: 20130316426Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.Type: ApplicationFiled: May 3, 2013Publication date: November 28, 2013Applicant: Genomatica, Inc.Inventor: Genomatica, Inc.
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Publication number: 20130316065Abstract: The purpose of the present invention is to provide a technique to duplicate and translocate any large region in a chromosome of a fungus belonging to Aspergillus across a wide range, so that it will possible to stably and systematically acquire an Aspergillus strain having a novel trait, which was un-acquirable by conventional techniques. The present invention relates to a transformant of the fungus belonging to Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5? or 3? end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3? or 5? end of its coding region is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.Type: ApplicationFiled: April 4, 2013Publication date: November 28, 2013Applicant: Kikkoman CorporationInventor: Tadashi TAKAHASHI
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Publication number: 20130309751Abstract: The present invention relates to isolated polypeptides having phospholipase C activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 9, 2011Publication date: November 21, 2013Applicant: Novozymes A/SInventors: Lan Tang, Ye Liu, Kim Borch, Jesper Brask
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Publication number: 20130309729Abstract: The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity.Type: ApplicationFiled: January 30, 2012Publication date: November 21, 2013Applicant: DSM IP ASSETS B.V.Inventors: Jan Metske Van Der Laan, Margot Elisabeth Francoise Schooneveld-Bergmans, Denise Ilse Jacobs
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Publication number: 20130309726Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: June 30, 2011Publication date: November 21, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen