Aspergillus Patents (Class 435/254.3)
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Publication number: 20140248673Abstract: This document describes biochemical pathways for producing pimelic acid, 7-hydroxyheptanoic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the CoA-dependent elongation enzymes or analog enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: September 4, 2014Applicant: INVISTA North America S.รก r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140248663Abstract: Provided are isolated polypeptides having cellobiohydrolase activity, catalytic domains and cellulose binding domains, and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: November 15, 2012Publication date: September 4, 2014Inventors: Lan Tang, Junxin Duan, Yu Zhang, Ye Liu
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Publication number: 20140248674Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: September 4, 2014Applicant: Novozymes A/SInventors: Sara Landvik, Lars H. Oestergaard, Lisbeth Kalum
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Publication number: 20140248397Abstract: A variant polypeptide having alpha-amylase activity, wherein the variant has an amino acid sequence which, when aligned with the alpha-amylase comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 4, 6, 13, 14, 15, 16, 20, 45, 47, 51, 54, 61, 68, 69, 70, 71, 72, 73, 74, 75, 77, 78, 80, 82, 87, 88, 94, 95, 100, 103, 104, 117, 124, 125, 126, 128, 130, 133, 134, 136, 143, 144, 146, 168, 174, 177, 178, 183, 186, 188, 189, 190, 194, 195, 199, 200, 201, 204, 207, 210, 214, 217, 219, 222, 225, 227, 233, 234, 235, 236, 240, 251, 252, 254, 258, 259, 260, 261, 262, 263, 264, 266, 267, 269, 271, 273, 281, 282, 283, 284, 286, 288, 299, 322, 323, 325, 327, 328, 331, 334, 350, 356, 358, 367, 370, 371, 374, 377, 378, 388, 391, 414, 421, 422, 445, 450, 467, 488, 505, 536, 548, 583, 588, 603, 637, 648, 651, 652, 660, 676, 677, said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered prType: ApplicationFiled: March 15, 2013Publication date: September 4, 2014Applicant: DSM IP ASSETS B.V.Inventors: Rene Marcel DE JONG, Hanna Elisabet ABBAS
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Publication number: 20140234231Abstract: Isolated ?-glucanases from Hypocrea tawa, Trichoderma reesei, and Trichoderma konilangbra are described, as well as oral care compositions containing the same. The oral care composition may be employed to prevent or reduce dental plaque.Type: ApplicationFiled: February 27, 2014Publication date: August 21, 2014Applicant: DANISCO US INC.Inventors: Steven Kim, Suzanne Lantz, Michael Pepsin
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Publication number: 20140234907Abstract: Provided are isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 16, 2012Publication date: August 21, 2014Applicant: Novozymes Inc.Inventors: Yu Zhang, Ye Liu, Junxin Duan, Lan Tang
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Publication number: 20140234897Abstract: Disclosed are isolated polypeptides having cellobiohydrolase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. Furthermore, nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains are disclosed.Type: ApplicationFiled: November 2, 2012Publication date: August 21, 2014Inventors: Yu Zhang, Junxin Duan, Ye Liu, Lan Tang
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Publication number: 20140234906Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 21, 2014Inventors: Carsten Andersen, Signe Eskildsen Larsen, Esben Peter Friis, Peter Kamp Hansen, Henrik Friis Madsen, Anders Viksoe Nielsen, Pernille Ollendorf Micheelsen
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Publication number: 20140234925Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.Type: ApplicationFiled: May 5, 2014Publication date: August 21, 2014Applicant: Novozymes, Inc.Inventors: Stephen Brown, Sheryl Luttringer, Debbie Yaver, Alan Berry
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Publication number: 20140234924Abstract: The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.Type: ApplicationFiled: October 4, 2012Publication date: August 21, 2014Inventors: Qingzhao Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140234918Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: August 21, 2014Applicant: Novozymes A/SInventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Publication number: 20140234902Abstract: Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways.Type: ApplicationFiled: May 7, 2014Publication date: August 21, 2014Applicant: GlycoFi, Inc.Inventor: Tillman U. Gerngross
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Publication number: 20140230096Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 24, 2013Publication date: August 14, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140227754Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: February 4, 2014Publication date: August 14, 2014Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Pieter van Solingen, Casper Vroemen
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Publication number: 20140227739Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: February 12, 2013Publication date: August 14, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140230098Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 27, 2013Publication date: August 14, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140227738Abstract: The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 21, 2012Publication date: August 14, 2014Applicant: Novozymes A/SInventors: Jeppe Wegener Tams, Tine Hoff, Morten Gjermansen, Peter Rahbek Oestergaard, Robert Piotr Olinski, Katrine Pontoppidan, Carsten Sjoeholm
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Publication number: 20140230097Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 24, 2013Publication date: August 14, 2014Inventor: Nikolaj Spodsberg
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Publication number: 20140220635Abstract: The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 17, 2012Publication date: August 7, 2014Applicant: Novozymes A/SInventors: Carsten Andersen, Signe Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoe Nielsen, Randall Deinhammer, Xinyu Shen
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Publication number: 20140220648Abstract: The invention relates to the production of aromatic molecules in prokaryotic and eukaryotic hosts such as E. coli, yeasts, filamentous fungi, algae, microalgae, other plant cells.Type: ApplicationFiled: June 22, 2012Publication date: August 7, 2014Applicant: RHO RenewablesInventor: Philip J. Barr
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Patent number: 8795676Abstract: The present invention discloses a codon-optimized gene encoding major capsid protein L1 of human papilloma virus, which is capable, after transduced into a yeast cell, of efficiently expressing the major capsid protein L1 of human papilloma virus. The present invention also discloses an immunogenic macromolecule which is essentially produced by expression of said codon-optimized gene encoding the major capsid protein L1 of human papilloma virus in a yeast cell. The present invention further discloses the use of said immunogenic macromolecule and a composition comprising said immunogenic macromolecule.Type: GrantFiled: November 24, 2008Date of Patent: August 5, 2014Assignee: Shanghai Zerun Biotechnology Co., Ltd.Inventors: Gaoxia Zhang, Qiong Shen, Jianqiang Lei, Jingyu Yuan, Menghua Zhang, Qianli Zhang, Yinghua Xiong, Roger Wei, Ke Wu
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Publication number: 20140206594Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 22, 2012Publication date: July 24, 2014Inventors: Martin Simon Borchert, Jeppe Wegener Tams
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Publication number: 20140205601Abstract: The present invention relates to amino acid sequences that are directed against RANK-L, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The invention also relates to nucleic acids encoding such amino acid sequences and polypeptides; to methods for preparing such amino acid sequences and polypeptides; to host cells expressing or capable of expressing such amino acid sequences or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells; and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.Type: ApplicationFiled: December 11, 2013Publication date: July 24, 2014Applicant: Ablynx N.V.Inventors: Els Anna Alice Beirnaert, Sigrid Cornelis, Hendricus Renerus Jacobus Mattheus Hoogenboom
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Publication number: 20140206045Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.Type: ApplicationFiled: March 31, 2014Publication date: July 24, 2014Applicant: Novozymes A/SInventors: Lan Tang, Wenping Wu, Junxin Duan, Pia Francke Johannesen
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Publication number: 20140206035Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.Type: ApplicationFiled: August 24, 2012Publication date: July 24, 2014Inventors: Tianqi Sun, Ming Li
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Publication number: 20140199754Abstract: The invention relates to a nucleic acid sequence encoding an itaconate transporting Major Facilitator Superfamily Transporter (MFST) gene sequence and the protein encoded thereby. Preferably said sequence is the nucleic acid that comprises the sequence of ATEG_09972.1 of Aspergillus terreus or homologues thereof. Overexpression of the protein enhances the production of itaconic acid in micro-organisms.Type: ApplicationFiled: January 6, 2014Publication date: July 17, 2014Applicant: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNOInventors: Maria Johanna VAN DER WERF, Peter Jan PUNT
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Publication number: 20140199293Abstract: Compositions and methods for making and using humanized anti-LPA monoclonal antibodies, and fragments and derivatives thereof, are described.Type: ApplicationFiled: November 19, 2013Publication date: July 17, 2014Inventors: Roger A. SABBADINI, Genevieve HANSEN, William A. GARLAND, Steven Tarran JONES, David Gareth WILLIAMS
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Publication number: 20140199737Abstract: This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.Type: ApplicationFiled: December 23, 2013Publication date: July 17, 2014Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140201871Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 25, 2014Publication date: July 17, 2014Applicant: Novozymes Inc.Inventor: Marc Dominique Morant
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Publication number: 20140193885Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbours other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.Type: ApplicationFiled: December 20, 2013Publication date: July 10, 2014Applicant: NEDERLANDSE ORGANISATIE VOOR TOEGEPAST- NATUURWETENSCHAPPELIJK ONDERZOEKInventors: Peter Jan PUNT, Maria Johanna VAN DER WERF
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Publication number: 20140193879Abstract: The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: March 13, 2014Publication date: July 10, 2014Applicant: Novozymes A/SInventors: Steffen Danielsen, Lars Kobberoee Skov, Ricardo Leite, Vincent Laize, M. Leonor Cancela Da Fonseca
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Publication number: 20140193862Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.รก r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193865Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.รก r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140193431Abstract: Disclosed are a human antibody comprising a human complementarity-determining region (CDR), which binds specifically to c-Met, and a framework region (FR), a polynucleotide encoding the human antibody, an expression vector comprising the polynucleotide, a transformant transformed with the expression vector, a method of producing the human antibody B7 by culturing the transformant, a wound healing composition comprising the human antibody as an active ingredient, a cell regeneration composition comprising the antibody as an active ingredient, and a drug conjugate comprising a drug linked to the human antibody. The c-Met-specific human antibody can function as an HGF mimic that can be used as a wound healing composition. The antibody can be widely used to determine the treatment and prognosis of various diseases, including neuronal infarction, progressive nephropathy, liver cirrhosis, lung fibrosis, kidney injury, liver injury, lung injury, and ulcerative wounds, which are treated by activation of HGF or c-Met.Type: ApplicationFiled: June 4, 2012Publication date: July 10, 2014Applicant: Korea Research Institute of Bioscience and BiotechnologyInventors: Young Woo Park, KiWon Jo, Chan Woong Park, Seok Ho Yoo, Myeoung Hee Jang, Hye Nan Kim, Seon Ha Yun, Kyu Won Cho, Mi Ra Park
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Publication number: 20140193863Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis.Type: ApplicationFiled: December 23, 2013Publication date: July 10, 2014Applicant: INVISTA North America S.รก r.I.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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Publication number: 20140186884Abstract: This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.Type: ApplicationFiled: July 23, 2013Publication date: July 3, 2014Applicant: BP Corporation North Americca Inc.Inventors: David Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, John Peter Flash Bartnek
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Publication number: 20140186902Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: July 3, 2014Applicant: INVISTA North America S. a r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140186913Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of an enoyl-CoA dehydratase, a 2-hydroxyacyl-CoA dehydratase, an isovaleryl-CoA/acyl-CoA dehydrogenase and a mevalonate diphosphate decarboxylase, or using recombinant host cells expressing one or more such enzymes.Type: ApplicationFiled: November 27, 2013Publication date: July 3, 2014Applicant: INVISTA North America S.รก r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140179588Abstract: The present invention relates to isolated polypeptides having cellulase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: June 26, 2014Applicant: Novozymes A/SInventors: Kirk Matthew Schnorr, Lars Anderson, Maria Leonor Quintais Cancela Da Fonseca, Ricardo Leite
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Publication number: 20140178949Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.Type: ApplicationFiled: November 15, 2013Publication date: June 26, 2014Applicant: DSM IP ASSETS B.V.Inventors: LIANG WU, AXEL CHRISTOPH TREFZER, STEFAAN MARIE ANDRE DE WILDEMAN, MARCO ALEXANDER VAN DEN BERG
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Publication number: 20140178947Abstract: Endoglucanase characterized by a decreased degree of activity inhibition by a lignin-derived aromatic compound, and prepared by substituting tryptophan at position 273 in the amino acid sequence of wild-type thermophilic bacterium-derived endoglucanase with an amino acid other than aromatic amino acids.Type: ApplicationFiled: March 4, 2014Publication date: June 26, 2014Applicants: National Institute of Advanced Industrial Science and Technology, Toray Industries, Inc.Inventors: Hiroyuki Kurihara, Takeshi Tsukada, Katsushige Yamada, Yumiko Mishima, Yuka Maeno, Kazuhiko Ishikawa
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Publication number: 20140178961Abstract: The present invention relates to a construct for accomplishing fixation of carbon dioxide and/or reduction of carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli), a vector comprising the construct, a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli) comprising the construct or being transformed with the vector, and a method for fixing carbon dioxide and/or reducing carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli).Type: ApplicationFiled: December 19, 2013Publication date: June 26, 2014Applicant: SHELL OIL COMPANYInventors: Xuefeng LU, Liu YANG, Zhengxu GAO, Xiaoming TAN
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Patent number: 8759023Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: January 26, 2012Date of Patent: June 24, 2014Assignee: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140170747Abstract: A cap has recess containing a porous frit and a graded porosity filter located so fluid from a container to which the cap is connected passes through the filter and out a spout on the cap. The filter porosity is selected to pass product expressed from cellular organisms grown in growth media inside the container while filtering out debris. A cap vent allows positive pressure in the container ullage to expedite filtering and reduce agitation of the container contents. This is not just a cap with a filter but an enabling tool. This tool enables total design change in many processes from previous thinking, allowing for the maximization of purified material, and reduction of multiple steps.Type: ApplicationFiled: October 15, 2013Publication date: June 19, 2014Applicant: Scientific Plastic Products, Inc.Inventors: Samuel A. Ellis, Kishan G. Hingorani
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Publication number: 20140170715Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: June 19, 2014Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Patent number: 8753850Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: GrantFiled: July 13, 2012Date of Patent: June 17, 2014Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Patent number: 8741626Abstract: The present invention relates to a mutant strain of Aspergillus sojae with an enhanced protease activity and a preparation method of a natural taste enhancer using the same. More specifically, the present invention relates to the mutant strain of Aspergillus sojae with an enhanced protease activity is obtained by selecting a strain with high protease activity and treating it with N-methyl-N?-Nitroso-N-nitrosoguanidine (NTG) and inducing a mutation through irradiating, and a preparation method of a natural taste enhancer using protein hydrolysate obtained by hydrolyzing the protein sources with their cultures.Type: GrantFiled: October 29, 2009Date of Patent: June 3, 2014Assignee: CJ Cheiljedang CorporationInventors: Hyun Chi, Seong-Jun Cho
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Patent number: 8741611Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.Type: GrantFiled: August 27, 2010Date of Patent: June 3, 2014Assignee: Novozymes, Inc.Inventors: Stephen Brown, Sheryl Luttringer, Debbie Yaver, Alan Berry
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Publication number: 20140147436Abstract: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.Type: ApplicationFiled: July 19, 2013Publication date: May 29, 2014Applicant: GENENTECH, INC.Inventor: Leonard G. PRESTA
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Publication number: 20140150137Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: April 26, 2012Publication date: May 29, 2014Applicant: Novozymes, Inc.Inventors: Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland