Abstract: An improved device, in the form of a container, for propagating explant cultures under sterile conditions while facilitating gas exchange with an outside environment, methods for employing the device, a process of manufacture to produce the device, a mold for use as part of the process of manufacture, and an apparatus for conducting the process of manufacture employing the mold.

Abstract: A process and device are disclosed for simultaneously cultivating different mammal cells, for separately obtaining different mammal cell products and for simulating organic interactions on the humoral plane. Essentially, the invention consists of arranging several culture vessels in a common supply circuit and or cultivating different mammal cells in separate vessels.

Abstract: A device for use in processing of a liquid and/or gas stream. The device is made up of a monolithic structure having active powder for treating streams. The structure has an inlet end and an outlet end and a multiplicity of cells extending from inlet end to outlet end. The cells are separated from one another by porous walls, a portion of the total number of cells being plugged in a pattern such that a stream enters the device through the unplugged cells at the inlet end and passes through at least two porous walls and the active powder in-between, and thereafter passes out of the device through unplugged cells at the outlet end. The device finds use in a number of applications including ion exchange, adsorption, biological and chemical reactions, and catalytic applications.

Abstract: A apparatus for assaying glycated proteins and other analytes in biological samples such as blood, in which a sample is presented to the apparatus, includes an inlet port between moveable between first and second inlets, such that the inlet can be brought into liquid communication with each inlet in turn. The inlet port accommodates a filter or binder. The apparatus also includes a microprocessor operable via a key pad, at least one light emitter and at least one light detector, a display and driver, and an A to D converter, and is operatively connected to a power source.

Abstract: A permeation cell for the in vitro determination of the permeation of pharmaceutical active ingredients through the skin, having a donor chamber (34) and an acceptor chamber (4) that is separable therefrom, a window lying between them for receiving sealingly a skin membrane wherein the acceptor chamber (4) is in the form of a cylindrical vessel that is upright during operation, having a closure (8; 50) at its upper end and a side window opening (6) in the region near its base, and the donor chamber (34) is in the form of a connection piece that joins onto the acceptor chamber radially and can be closed at its outer end. The acceptor chamber (4) is inserted removably into a housing (10), which housing has, in the region of the window opening (6) of the acceptor chamber, a corresponding slightly wider opening (30), and the connection piece forming the donor chamber (34) is screwed removably onto the housing concentrically with the window opening (6).

Abstract: Cartilage tissue and implants comprising tissue are produced in vitro starting from cells having the ability to form an extracellular cartilage matrix. Such cells are brought into a cell space (1) and are left in this cell space for producing an extracellular cartilage matrix. The cells are brought into the cell space to have a cell density of ca. 5×107 to 109 cells per cm3 of cell space. The cell space (1) is at least partly separated from a culture medium space (2) surrounding the cell space by means of a semi-permeable wall (3) or by an open-pore wall acting as convection barrier. The open-pore wall can be designed as a plate (7) made of a bone substitute material and constituting the bottom of the cell space (1). The cells settle on such a plate (7) and the cartilage tissue growing in the cell space (1).

Abstract: Cartilage tissue and implants comprising cartilage tissue are produced in vitro starting from cells having the ability to form an extracellular cartilage matrix. Such cells are brought into a cell space (1) and are left in this cell space for producing an extracellular cartilage matrix. The cells are brought into the cell space to have a cell density of ca. 5×107 to 109 cells per cm3 of cell space. The cell space (1) is at least partly separated from a culture medium space (2) surrounding the cell space by a semi-permeable wall (3) or by an open-pore wall acting as convection barrier. The open-pore wall can be designed as a plate (7) made of a bone substitute material and constituting the bottom of the cell space (1).

Abstract: The invention relates to a bioreactor for a cell treatment of a medium. Said bioreactor comprises an element defining a chamber in which cells for treating the medium are located, a liquid permeable membrane separating the said chamber from a first channel in which flows the medium to be treated, and a gas permeable membrane separating the said chamber from a second channel in which flows a gas containing oxygen.

Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media; allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

Abstract: A patient replica is created from layered culture medium where solid culture medium is formed so that a discontinuity exists, for example, between layers of culture medium or between culture medium and the container holding the medium. An injection port is provided in registration with the discontinuity so that a sample of patient blood can be infused into the discontinuity to form a thin layer of blood in contact with a surface of culture medium. The thin layer of blood obviates the requirement for any anticoagulant allowing blood-borne pathogens to be readily cultured without using broth. Further antibiotics or other drug samples may be placed on an exposed surface of the culture medium above the blood layer so that the antibiotic can diffuse through the culture medium and reveal the sensitivities of the cultured pathogens.

Abstract: The invention relates to a water supply and drainage type microorganic deodorizing system, by which a clogging of deodorizer can be effectively prevented in order to decrease a deodorizing capacity, wherein deodorization chambers are provided in two stages in the tower main body, offensive odor constituents are gradually adsorbed by filters of the deodorization chambers as ordered by decomposing action of microorganisms, and decomposed in order to completely deodorize. Furthermore, by providing a water sprinkling system of replenishing water in relation to the filters the performance of the filters can be restored, and furthermore, by providing a water diluting system WC which dilutes cleansing water of the filters, drainage can be prevented from being contaminated, in accordance with drainage standards.