Support Is A Fiber Patents (Class 435/398)
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Publication number: 20130023050Abstract: The invention provides a method of culturing a corneal endothelial cell by use of a culture medium containing an ascorbic acid derivative and the like.Type: ApplicationFiled: February 4, 2011Publication date: January 24, 2013Applicant: FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATIONInventors: Nobuyuki Shima, Miwa Kimoto, Masahiro Yamaguchi, Satoru Yamagami
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Publication number: 20120282228Abstract: The present invention provides a method of producing progenitor cells, such as cells capable of being differentiated into a plurality of different cell types, from differentiated cells. Methods of using progenitor cells in differentiation and/or tissue or organ repair and/or regeneration and/or building are also provides. Methods of using progenitor cells in treatment and prophylaxis of conditions alleviated by administering stem cells or tissue or organ derived from stem cells to a subject or by grafting stem cells or tissue or organ derived from stem cells into a subject or by transplanting stem cells or tissue or organ derived from stem cells into a subject are also provided. Also included are progenitor cells and differentiated cells and/or tissues and/or organs derived therefrom, and kits comprising same.Type: ApplicationFiled: July 15, 2010Publication date: November 8, 2012Inventor: Vishal Bhasin
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Patent number: 8293531Abstract: Disclosed are three-dimensional (3D) systems as may be utilized for ex vivo tissue or cell growth and development. A system generally includes a base material and at least one wicking fiber embedded therein through which a liquid can be spontaneously drawn by capillary action. Wicking fibers can define a plurality of colinear channels along the exterior surface of the axial length of the fiber. Wicking fibers can be present in disclosed systems as individual fibers or in bundles. Disclosed systems can be useful in various scientific studies, including, but not limited to, drug discovery, vaccine development, cell biology studies, and biomaterial development.Type: GrantFiled: August 29, 2008Date of Patent: October 23, 2012Assignee: Clemson University Research FoundationInventors: Karen J. L. Burg, Chih-Chao Yang
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Publication number: 20120232588Abstract: A thread, in particular surgical thread includes a cell-retaining structure and cells and a method for manufacturing the thread.Type: ApplicationFiled: June 25, 2010Publication date: September 13, 2012Applicant: AESCULAP AGInventors: Ithamar Stocchero, Erich Odermatt, Lutz Funk, Volker Friedrich, Marta Casanovas Albalate
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Publication number: 20120225039Abstract: Cartilage has been constructed using biodegradable electrospun polymeric scaffolds seeded with chondrocytes or adult mesenchymal stem cells. More particularly engineered cartilage has been prepared where the cartilage has a biodegradable and biocompatible nanofibrous polymer support prepared by electrospinning and a plurality of chondocytes or mesenchymal stem cells dispersed in the pores of the support. The tissue engineered cartilages of the invention possess compressive strength properties similar to natural cartilage. Methods of preparing engineered tissues, including tissue engineered cartilages, are provided in which an electrospun nanofibrous polymer support is provided, the support is treated with a cell solution and the polymer-cell mixture cultured in a rotating bioreactor to generate the cartilage. The invention provides for the use of the tissue engineered cartilages in the treatment of cartilage degenerative diseases, reconstructive surgery, and cosmetic surgery.Type: ApplicationFiled: May 15, 2012Publication date: September 6, 2012Applicant: The Government of the United States of America, as represented by the Secretary,Department of HealthInventors: Wan-Ju Li, Rocky S. Tuan
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Publication number: 20120225484Abstract: The present invention provides compositions comprising human placental telopeptide collagen, methods of preparing the compositions, methods of their use and kits comprising the compositions. The compositions, kits and methods are useful, for example, for augmenting or replacing tissue of a mammal.Type: ApplicationFiled: May 9, 2012Publication date: September 6, 2012Inventors: Mohit Bhatia, Christ Lugo, Qian Ye, James W. Edinger
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Patent number: 8198086Abstract: The present invention provides a method for arranging various cells as cell clusters in an arbitrary three-dimensional space and producing a three dimensional structure of a desired shape constituted exclusively by cells. Furthermore, the present invention provides a support provided with a substrate and a thread or needle-shaped material that penetrates the substrate and cell clusters for positioning cell clusters in arbitrary space. The support is provided with a sheet that can be removed as necessary for covering the substrate. Further, a method for using the support structure to position cell clusters in an arbitrary space and a method for the production of three-dimensional cell structures are provided.Type: GrantFiled: March 31, 2008Date of Patent: June 12, 2012Assignee: Kyushu University, National University CorporationInventors: Toshinobu Koga, Soichi Nagasato, Yukihide Iwamoto, Koichi Nakayama
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Publication number: 20120135448Abstract: The present invention provides methods and devices for the fabrication of 3D polymeric fibers having micron, sub-micron, and nanometer dimensions, as well as methods of use of these polymeric fibers.Type: ApplicationFiled: May 13, 2010Publication date: May 31, 2012Applicant: President and Fellows of Harvard CollegeInventors: Kevin Kit Parker, Mohammad Reza Badrossamay, Josue Adrian Goss
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Patent number: 8183033Abstract: The invention relates to methods for preparing and performing quantitative PCR analyses, a new sealing device and a new use. According to the invention, a sample vessel containing the samples to be analyzed is sealed by placing a planar sealing device on the vessel to cover the samples and applying pressure on the sealing device in order to deform the sealing device so as to form a light-refracting geometry individually for the samples to be analyzed. The invention offers a convenient way of sealing the vessel and forming analysis-improving optical lenses over the samples simultaneously.Type: GrantFiled: March 21, 2008Date of Patent: May 22, 2012Assignee: Bioinnovations OyInventor: Bruce R Turner
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Publication number: 20120100203Abstract: Fabrication of yarns or other shaped articles from materials in powder form (or nanoparticles or nanofibers) using carbon nanotube/nanofiber sheet as a platform (template). This includes methods for fabricating biscrolled yarns using carbon nanotube/nanofiber sheets and biscrolled fibers fabricated thereby.Type: ApplicationFiled: May 27, 2010Publication date: April 26, 2012Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Shaoli Fang, Marcio Dias Lima, Xavier N. Lepro-Chavez, Javier Carretero-Gonzalez, Elizabeth Castillo-Martinez, Raquel Ovalle-Robles, Carter Sebastian Haines, David Michael Novitski, Mohammad H. Haque, Chihye Lewis-Azad, Mikhail Kozlov, Anvar A. Zakhidov, Ray H. Baughman
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Patent number: 8110385Abstract: A linear biodevice is provided with fibers that have a curved portion in its cross section approximately perpendicular to the longitudinal direction and approximately spherical adhesive cells that adhere to the periphery of the fiber. A membrane-like biodevice is provided with a sheet that has an opening and approximately spherical adhesive cells that adhere to the opening. A curved portion is present in the cross section on an inner edge of the opening. A bioreactor is provided with a membrane-like biodevice in which spherical adhesive cells grow densely so as to close the openings of the sheet.Type: GrantFiled: June 30, 2005Date of Patent: February 7, 2012Assignee: Japan Agency for Marine-Earth Science and TechnologyInventors: Satoshi Konishi, Tetsuya Miwa
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Publication number: 20110294214Abstract: The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability.Type: ApplicationFiled: August 11, 2011Publication date: December 1, 2011Inventors: Alireza Rezania, Ragae Ghabrial
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Publication number: 20110263021Abstract: A compact, high-yield, bio-reactor for use as small, medium and large-scale production unit for therapeutic proteins, proteins in general and enzymes based on controlled activity of the cells or micro organisms and the production of such as high value antibodies for pharmaceutical and/or bio-diagnostic applications.Type: ApplicationFiled: December 18, 2009Publication date: October 27, 2011Applicant: STOBBE TECH A/SInventor: Per Stobbe
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Publication number: 20110250689Abstract: The invention relates a multilayer preform obtained by electro-spinning, which preform is suitable as a scaffold for a prosthesis, which preform comprises at least one layer of microfibres and at least one layer of nanofibres, wherein the pore size of the at least one layer of microfibres is in the range of 1-300 micrometre and in that the pore size of the at least one layer of nanofibres is in the range of 1-300 micrometre. The present invention also relates to a method of producing said preform. The present invention also relates to the use of the present preform as a substrate for growing human or animal tissue thereon. The present invention furthermore relates to a method for growing human or animal tissue on a substrate, wherein the present preform is used as the substrate.Type: ApplicationFiled: October 9, 2009Publication date: October 13, 2011Inventors: Franciscus Petrus Thomas Baaijens, Mirjam Petronella Rubbens, Martijn Antonius Johannes Cox
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Publication number: 20110236974Abstract: The present invention provides methodologies and parameters for fabrication of the hybrid biomaterial by blending pure laminin or complex extracts of tissues containing laminin with biopolymers such as polycaprolactone (PCL), polylactic/polyglycolic acid copolymer (PLGA) or Polydioxanone (PDO) in fluoroalcohols (HFP, TFA), fabrication of substrates and scaffolds and devices from the hybrid biomaterial in forms such as films, nanofibers by electrospinning or microspheres, and the biological or biomedical use of the material or devices derived from it.Type: ApplicationFiled: March 10, 2011Publication date: September 29, 2011Applicant: University of Virginia Patent FoundationInventors: Roy Clinton Ogle, Edward A. Botchwey, III, Rebekah A. Neal
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Publication number: 20110230411Abstract: Provided are method of generating a fiber from a globular protein such as albumin. Also provided are albumin fibers and fabrics and methods of using same for bonding a damaged tissue or for ex vivo or in vivo formation of a tissue.Type: ApplicationFiled: January 31, 2008Publication date: September 22, 2011Applicant: ZVI NEVOInventors: Eyal Zussman, David Simhon, Shmuel Chervinsky, Abraham Katzir, Zvi Nevo, Yael Dror
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Publication number: 20110189773Abstract: The present invention provides a novel silk-fiber-based matrix having a wire-rope geometry for use in producing a ligament or tendon, particularly an anterior cruciate ligament, ex vivo for implantation into a recipient in need thereof. The invention further provides the novel silk-fiber-based matrix which is seeded with pluripotent cells that proliferate and differentiate on the matrix to form a ligament or tendon ex vivo. Also disclosed is a bioengineered ligament comprising the silk-fiber-based matrix seeded with pluripotent cells that proliferate and differentiate on the matrix to form the ligament or tendon. A method for producing a ligament or tendon ex vivo comprising the novel silk-fiber-based matrix is also disclosed.Type: ApplicationFiled: March 29, 2011Publication date: August 4, 2011Applicant: ALLERGAN, INC.Inventors: Gregory H. Altman, David L. Kaplan, Rebecca L. Horan, David J. Horan
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Publication number: 20110183419Abstract: The invention concerns a novel silica sol material and its use for producing bioabsorbable and biodegradable silica gel materials having improved properties. The materials such as for example fibres, fibrous nonwoven webs, powders, monoliths and/or coatings are used, for example, in medical technology and/or human medicine, in particular for wound treatment.Type: ApplicationFiled: January 10, 2008Publication date: July 28, 2011Applicant: Bayer Innvation GmbHInventor: Walther Glaubitt
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Publication number: 20110135707Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided for use in numerous biomedical applications. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. PHAs are provided which are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone and other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.Type: ApplicationFiled: February 4, 2011Publication date: June 9, 2011Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
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Patent number: 7955867Abstract: In the present invention cells are placed in a multiwell plate and grown. When the assay is to be performed, one uses gravity to wash away any unbound ligands rather than vacuum or centrifugation. The cells are then examined to detect the bound ligand. To perform the washing step(s) the plate is placed into a carrier plate having open wells in register with the wells of the filter plate or one may use a wicking device or an underdrain attached to the bottom of the filter plate. Sufficient wash liquid is added to allow for filtration by the effect of gravity to occur. Cells are retained within the wells at a rate of 4 times that of other rapid methods.Type: GrantFiled: January 18, 2008Date of Patent: June 7, 2011Assignee: Millipore CorporationInventor: Jun Y. Park
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Patent number: 7939296Abstract: Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.Type: GrantFiled: May 15, 2009Date of Patent: May 10, 2011Assignee: JCR Pharmaceuticals Co., Ltd.Inventors: Atsushi Sugimura, Katsuya Daimon, Kazutoshi Mihara, Yae Ito
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Publication number: 20110076771Abstract: The present disclosure relates to a fiber, a method of forming a fiber, a system for forming a fiber, and a method of engineering tissue from a fiber. The fiber includes an engineered geometric feature forming a non-Euclidian geometry.Type: ApplicationFiled: September 23, 2010Publication date: March 31, 2011Applicants: ARMARK AUTHENTICATION TECHNOLOGIES, LLC, HILLS INC.Inventors: Peter D. GABRIELE, Jeffrey H. ROBERTSON, Jeffrey S. HAGGARD
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Publication number: 20110038914Abstract: An isolated collagen fiber is disclosed, wherein a length of the fiber prior to stretching by about 15%, is identical to a length of the fiber following said stretching by about 15%. The fiber comprises a Nuclear Magnetic Resonance (NMR) spectroscopic profile as shown in FIG. 1. Uses thereof and method of isolating are also disclosed.Type: ApplicationFiled: March 25, 2009Publication date: February 17, 2011Applicants: RAMOT AT TEL AVIV UNIVERSITY LTD., TECHNION RESEARCH & DEVELOPMENT FOUNDATION LTD.Inventors: Yehuda Benayahu, Dafna Benayahu, Yoel Kashman, Amira Rudi, Yoram Lanir, Ido Sella, Einat Raz
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Publication number: 20110033927Abstract: Methods for generating small diameter tissue engineered blood vessels through direct cell seeding onto tubular templates or mandrels, such as fibrin microthreads or collagen-coated silicon tubes, are described.Type: ApplicationFiled: April 1, 2010Publication date: February 10, 2011Applicant: WORCESTER POLYTECHNIC INSTITUTEInventors: Marsha Rolle, Darshan P. Parekh, Kshama J. Doshi, Tracy Gwyther
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Publication number: 20100331980Abstract: Provided herein are anisotropic muscle implants that have a biodegradable scaffold comprising a plurality of fibers oriented along a longitudinal axis. The implants may include mammalian muscle cells seeded and/or fused into myotubes on the scaffold. Methods of forming the muscle implants are provided, as are methods of treating a subject in need of skeletal muscle reconstruction.Type: ApplicationFiled: January 30, 2009Publication date: December 30, 2010Inventors: Sang Jin Lee, James J. Yoo, Anthony Atala
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Publication number: 20100324697Abstract: Provided is an electroactive structure for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.Type: ApplicationFiled: March 12, 2010Publication date: December 23, 2010Applicant: New Jersey Institute of TechnologyInventors: Treena Arinzeh, George Collins, Yee-Shuan Lee
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Publication number: 20100297768Abstract: A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.Type: ApplicationFiled: April 26, 2010Publication date: November 25, 2010Applicant: Michigan State UniversityInventors: Melvin S. Schindler, Hoo Young Chung
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Publication number: 20100278798Abstract: Methods and systems forming biocompatible materials are disclosed herein. Forming a biocompatible material may include contacting a liquid, having a linking material, with an adjoining material having embedded therein a nucleating material that causes the linking material to nucleate and grow into the liquid. After a time sufficient to cause the linking material to grow substantially from the nucleating material into a space occupied by the liquid, the liquid may be solidified to form a solid such that the linking material secures the solid to the adjoining material.Type: ApplicationFiled: September 29, 2008Publication date: November 4, 2010Applicant: The Trustees of Columbia University in the City of New YorkInventors: Samuel K. Sia, Brian Michael Gillette
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Patent number: 7795027Abstract: Described are preferred extracellular matrix composites including a first extracellular matrix material having a second extracellular matrix material deposited thereon. The preferred materials are made by culturing cells in contact with an extracellular matrix graft material in a fashion to cause the cells to biosynthesize and deposit extracellular matrix components on the material. The cells are then removed to provide the extracellular matrix composite material.Type: GrantFiled: March 3, 2006Date of Patent: September 14, 2010Assignee: Cook Biotech IncorporatedInventor: Michael C Hiles
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Publication number: 20100196333Abstract: The present invention is directed to a fiber, preferably bone fiber, having a textured surface, which acts as an effective binding substrate for bone-forming cells and for the induction or promotion of new bone growth by bone-forming cells, which bind to the fiber. Methods of using the bone fibers to induce or promote new bone growth and bone material compositions comprising the bone fibers are also described. The invention further relates to a substrate cutter device and cutter, which are effective in producing substrate fibers, such as bone fibers.Type: ApplicationFiled: January 25, 2010Publication date: August 5, 2010Applicant: LifeNet HealthInventors: Barton D. Gaskins, Dennis L. Phelps, Daniel B. Osborne, Louis E. Ford, Lloyd Wolfinbarger, JR.
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Publication number: 20100168872Abstract: This invention relates to biomaterials for directional tissue growth which comprise soluble fibres having a variable cross-sectional area which dissolve in a directional manner in situ, thereby controlling the direction and rate of formation of microchannels within the biomaterial and allowing vectored and timed channelling through the biomaterial. This may be useful, for example in directing the growth of blood vessels, nerves and other repair cells through the biomaterial in defined directions.Type: ApplicationFiled: September 3, 2008Publication date: July 1, 2010Inventors: Robert BROWN, Tijna ALEKSEEVA
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Publication number: 20100151575Abstract: Methods of making conditioned media by culturing cells on nonwoven substrates are disclosed. More specifically, methods of making conditioned media by culturing mammalian kidney-derived cells on nonwoven substrates are disclosed.Type: ApplicationFiled: December 15, 2008Publication date: June 17, 2010Inventors: David C. Colter, Christian Kazanecki, Brian C. Kramer, Jackie J. Donners
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Patent number: 7704740Abstract: A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.Type: GrantFiled: November 5, 2003Date of Patent: April 27, 2010Assignee: Michigan State UniversityInventors: Melvin S. Schindler, Hoo Young Chung
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Patent number: 7678573Abstract: A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.Type: GrantFiled: April 11, 2005Date of Patent: March 16, 2010Assignee: Pluristem Ltd.Inventors: Shoshana Merchav, Shai Meretski, Dov Zipori, Avinoam Kadouri
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Patent number: 7622299Abstract: In one aspect, the invention provides methods for forming a target tissue substitute. The methods of the invention comprise the following steps: (a) providing a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is designed to mimic the configuration of one or more structural elements in a target tissue; and (b) culturing cells on the scaffold to form a target tissue substitute. In another aspect, the invention provides implantable medical devices. The implantable medical devices of the invention comprise a scaffold comprising one or more layers of one or more arrays of microfibers, wherein one or more of the arrays of microfibers is arranged to mimic the configuration of one or more structural elements in a target tissue. Typically, cells are cultured on the scaffold to form a target tissue substitute.Type: GrantFiled: February 24, 2003Date of Patent: November 24, 2009Assignee: University of WashingtonInventors: Joan E. Sanders, Thomas Neumann
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Patent number: 7615373Abstract: The invention is directed to formation and use of electroprocessed collagen, including use as an extracellular matrix and, together with cells, its use in forming engineered tissue. The engineered tissue can include the synthetic manufacture of specific organs or tissues which may be implanted into a recipient. The electroprocessed collagen may also be combined with other molecules in order to deliver substances to the site of application or implantation of the electroprocessed collagen. The collagen or collagen/cell suspension is electrodeposited onto a substrate to form tissues and organs.Type: GrantFiled: May 28, 2003Date of Patent: November 10, 2009Assignees: Virginia Commonwealth University Intellectual Property Foundation, Organogenesis, Inc.Inventors: David G. Simpson, Gary L. Bowlin, Gary E. Wnek, Peter J. Stevens, Marcus E. Carr, Jamil A. Matthews, Saravanamoorthy Rajendran
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Publication number: 20090148947Abstract: The present invention relates to culturing cells utilizing a matrix of microfibrillated thermoplastic polymeric materials. More specifically, the present invention relates to a method of culturing cells. In addition, the invention relates to a microfibrillated article for culturing cells dispersed in a cell culture medium. The matrix of thermoplastic polymeric materials for culturing cells of this invention finds use in tissue engineering and wound healing applications.Type: ApplicationFiled: February 13, 2009Publication date: June 11, 2009Inventors: Mario A. Perez, Terry R. Hobbs, Stephanie J. Moeller
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Publication number: 20090137043Abstract: The present subject matter relates to the modification of fibers by the growth of films by the Atomic Layer Epitaxy (ALE) process, which is also commonly referred to as Atomic Layer Deposition (ALD). The presently disclosed subject matter relates in particular to a process for the modification of the surface and bulk properties of fiber and textile media, including synthetic polymeric and natural fibers and yarns in woven, knit, and nonwoven form by low-temperature ALD.Type: ApplicationFiled: November 25, 2008Publication date: May 28, 2009Inventors: Gregory N. Parsons, Gary Kevin Hyde, Joseph C. Spagnola, Qing Peng
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Patent number: 7534609Abstract: A method of expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) obtaining the undifferentiated hemopoietic stem cells; and (b) culturing the undifferentiated hemopoietic stem cells in a medium containing a stromal cell conditioned medium, the stromal cell conditioned medium being derived from a stationary phase plug-flow bioreactor in which a three dimensional stromal cell culture has been established under continuous flow of a culture medium on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding the undifferentiated hemopoietic stem cells.Type: GrantFiled: April 11, 2005Date of Patent: May 19, 2009Assignee: Pluristem Life Systems Inc.Inventors: Shoshana Merchav, Shai Meretski
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Publication number: 20090075382Abstract: The invention provides a fibre-reinforced scaffold for tissue engineering. The scaffold comprises a matrix comprising a biocompatible polymer, the matrix having a, porous structure; and discrete, macroscopic fibres embedded within the matrix, wherein the fibres are oriented such that at least one mechanical property of the scaffold is anisotropic. The invention further relates to fibre-reinforced films and to processes for producing such scaffolds and films.Type: ApplicationFiled: March 13, 2007Publication date: March 19, 2009Inventor: Eleftherios Sachlos
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Publication number: 20090035858Abstract: Cell cultures or tissue engineering supports, include at least a porous matrix based on a collagen sponge which defines first pores and a porous three-dimensional knit which defines second pores, the porous matrix filling the three-dimensional knit and all the first and second pores being at least partially interconnected with one another.Type: ApplicationFiled: July 30, 2007Publication date: February 5, 2009Inventors: Yves Bayon, Philippe Gravagna, Alfredo Meneghin, Michel Therin, Olivier Lefranc
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Publication number: 20080299657Abstract: The present invention relates to nanofibers. In particular, the present invention provides aligned nanofiber bundle assemblies. In some embodiments, the aligned nanofiber bundle assemblies are used for tissue regeneration, controlled growth of cells, and related methods (e.g., diagnostic methods, research methods, drug screening).Type: ApplicationFiled: February 14, 2008Publication date: December 4, 2008Applicant: Northwestern UniversityInventors: Samuel I. Stupp, Shuming Zhang, Alvaro Mata, Megan A. Greenfield
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Publication number: 20080213389Abstract: Methods of making a biologically active three-dimensional scaffold capable of supporting growth and differentiation of a cell are described. Biologically active three-dimensional scaffold made by the methods of the invention and an engineered tissue made from the scaffolds are described. Fibers of desired porosity can be obtained from non-structural ECM by lyophilization and/or electrospinning which can be useful for numerous tissue engineering applications requiring complex scaffolds, such as wound healing, artificial skin (burns), soft tissue replacement/repair and spinal cord injury.Type: ApplicationFiled: June 19, 2006Publication date: September 4, 2008Applicant: DREXEL UNIVERSITYInventors: Peter I. Lelkes, Mengyan Li, Anat Perets, Honesto Poblete, Philip Lazarovici
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Publication number: 20080206343Abstract: Provided herein are methods and compositions for the production of hepatocytes from placenta stem cells. Further provided herein is the use of such hepatocytes in the treatment of, and intervention in, for example, trauma, inflammation, and degenerative disorders of the liver. Also provided herein are compositions and methods relating to combinations of nanofibrous scaffolds and adherent placental stem cells and methods of using the same in cartilage repair. Finally, provided herein are compositions and methods relating to nonadherent, CD34+CD45? stem cells from placenta.Type: ApplicationFiled: February 12, 2008Publication date: August 28, 2008Inventors: James W. Edinger, Robert J. Hariri, Jia-Lun Wang, Qian Ye, Marian Pereira, Sascha Dawn Abramson, Kristen S. Labazzo
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Publication number: 20080176213Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.Type: ApplicationFiled: March 27, 2008Publication date: July 24, 2008Applicant: INVITROGEN CORPORATIONInventors: John Matthew Mauro, Thomas Harry Steinberg, Lawrence I. Greenfield, Louis Leong
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Publication number: 20080153077Abstract: Described herein are substrates for immobilizing cells and tissues and methods of use thereof.Type: ApplicationFiled: June 8, 2007Publication date: June 26, 2008Inventor: David Henry
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Publication number: 20080131965Abstract: The invention relates to a method for producing a preform by means of an electrospinning process. The present invention also relates to the use of the present preform as a substrate for growing human or animal tissue thereon. The present invention furthermore relates to a method for growing human or animal tissue on a substrate, wherein the present preform is used as the substrate.Type: ApplicationFiled: April 28, 2005Publication date: June 5, 2008Inventor: Franciscus Petrus Thomas Baaijens
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Publication number: 20080124766Abstract: To provide an implant material which exhibits relatively high mechanical binding with an osteoblast and also high strength. [MEANS FOR SOLVING PROBLEMS] A scaffold material (10) capable of inducing a biological hard tissue, which comprises a rod (11) having a trunk portion (21) and bride girders (22), a binding layer (13) formed on the periphery of the rod and a metal fiber layer (14) formed on the periphery of the binding layer, and which further has a reinforcing layer (15) formed on the periphery of the metal fiber layer (14). The binding layer (13) has pores having an average pore size of less than 100 m, and the metal fiber layer (14) has pores having an average pore size of 100 to 400 m.Type: ApplicationFiled: September 26, 2005Publication date: May 29, 2008Inventors: Yoshinori Kuboki, Yasuo Seki, Hiroyuki Shiota
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Patent number: RE42479Abstract: It has been discovered that improved yields of engineered tissue following implantation, and engineered tissue having enhanced mechanical strength and flexibility or pliability, can be obtained by implantation, preferably subcutaneously, of a fibrous polymeric matrix for a period of time sufficient to obtain ingrowth of fibrous tissue and/or blood vessels, which is the removed for subsequent implantation at the site where the implant is desired. The matrix is optionally seeded prior to the first implantation, after ingrowth of the fibrous tissue, or at the time of reimplantation. The time required for fibrous ingrowth typically ranges from days to weeks. The method is particularly useful in making valves and tubular structures, especially heart valves and blood vessels.Type: GrantFiled: February 19, 2004Date of Patent: June 21, 2011Assignee: Children's Medical Center CorporationInventors: Joseph P. Vacanti, Christopher K. Breuer, Berverly E. Chaignaud, Toshiraru Shin'oka
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Patent number: RE42575Abstract: It has been discovered that improved yields of engineered tissue following implantation, and engineered tissue having enhanced mechanical strength and flexibility or pliability, can be obtained by implantation, preferably subcutaneously, of a fibrous polymeric matrix for a period of time sufficient to obtain ingrowth of fibrous tissue and/or blood vessels, which is the removed for subsequent implantation at the site where the implant is desired. The matrix is optionally seeded prior to the first implantation, after ingrowth of the fibrous tissue, or at the time of reimplantation. The time required for fibrous ingrowth typically ranges from days to weeks. The method is particularly useful in making valves and tubular structures, especially heart valves and blood vessels.Type: GrantFiled: September 28, 2006Date of Patent: July 26, 2011Assignee: Children's Medical Center CorporationInventors: Joseph P. Vacanti, Christopher K. Breuer, Berverly E. Chaignaud, Toshiraru Shin'oka