Involving A Micro-organism Or Cell Membrane Bound Antigen Or Cell Membrane Bound Receptor Or Cell Membrane Bound Antibody Or Microbial Lysate Patents (Class 435/7.2)
Animal cell (Class 435/7.21)
Flagellar-antigen or pili-antigen (Class 435/7.3)
Fungi (e.g., yeast, mold, etc.) (Class 435/7.31)
Bacteria or actinomycetales (Class 435/7.32)
  • Method of rapidly detecting microorganisms using nanoparticles
    Patent number: 8597897
    Abstract: The present invention relates to a method of rapidly detecting microorganisms using nanoparticles, and more particularly to a method and device of rapidly detecting microorganisms by adding, to the microorganisms to be detected, nanoparticles having immobilized thereon an antibody that binds specifically to the microorganisms to be detected, subjecting the mixture to an immune reaction to form a reaction solution, passing the reaction solution through a microorganism-concentrating film to concentrate the microorganisms, capturing microorganisms, which was immune-reacted with the antibody-immobilized nanoparticles, by a microorganism-capturing filtration membrane, and determining the presence and concentration of the microorganisms.
    Type: Grant
    Filed: November 24, 2010
    Date of Patent: December 3, 2013
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Min Gon Kim, Yong Beom Shin, Yoon Joo Sung
  • Recombinant fusion protein and polynucleotide construct for immunotoxin production
    Patent number: 8597655
    Abstract: The present invention relates to a polynucleotide construct encoding a fusion protein consisting of a domain which binds the immunoglobulin Fc region, genetically fused to a truncated form of Pseudomonas exotoxin A (PE). In particular, the invention discloses the fusion protein, ZZ-PE38, and further provides immunotoxins, formed from complexes of the fusion protein with antibodies for targeted cell killing.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: December 3, 2013
    Assignees: Ramot at Tel-Aviv University Ltd., The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center
    Inventors: Itay Barnea, Itai Benhar, Rahamim Ben-Yosef, Akiva Vexler
  • EXPRESSION OF SECRETED AND CELL-SURFACE POLYPEPTIDES
    Publication number: 20130316366
    Abstract: Some embodiments herein provide compositions and methods for expressing secreted and cell-surface-bound polypeptides in a single cell. In some embodiments, secreted and cell-surface polypeptide are produced from a single polynucleotide. The polynucleotide can comprise a sequence (or sequence encoding a polypeptide) that mediates separation of a membrane anchor from the polypeptide. In some embodiments, a desired ratio of secreted to surface-bound polypeptide is obtained by selecting a sequence that mediates a desired level of separation of the membrane anchor from the polypeptide.
    Type: Application
    Filed: May 24, 2013
    Publication date: November 28, 2013
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Kenneth Yu, David Baltimore, Lili Yang
  • Method for Detecting and Quantifying Microorganisms
    Publication number: 20130316333
    Abstract: The present invention relates to a method for detecting at least one microorganism in a sample, including: cultivating the sample in a liquid medium in the presence of at least one specific ligand of the microorganism, and at least one scavenger having a lower affinity to the microorganism than the ligand, the binding of a compound to the ligand producing a first measurable signal and the binding of a compound to the scavenger producing a second measurable signal; determining the values of the first and second signals for at least one cultivation period; wherein it is deduced that the sample includes the microorganism when the values of the first signal and second signal are different for the same cultivation period.
    Type: Application
    Filed: November 30, 2011
    Publication date: November 28, 2013
    Applicant: Commissariat A L'Energie Atomique Et Aux Energies Alternatives
    Inventors: Yoann Roupioz, Roberto Calemczuk, Thierry Vernet, Thierry Livache, Sihem Bouguelia, Claire Durmort
  • Discrete Contact MR Bio-Sensor with Magnetic Label Field Alignment
    Publication number: 20130316367
    Abstract: The invention describes a family of sensors for assaying macro-molecules and/or biological cells in solution. The invention also describes methods of making and using the sensors. Each sensor has the form of a well (a hollow cylinder having a floor but no lid) or a trench whose walls comprise a plurality of GMR or TMR devices. Suitably shaped magnets located below each well's floor pull labeled particles into the well/trench and up against the inner wall where a field gradient orients them for optimum detection. Any unattached labels that happen to also be in the well/trench are removed through suitably sized holes in the floor.
    Type: Application
    Filed: July 31, 2013
    Publication date: November 28, 2013
    Applicant: Headway Technologies, Inc.
    Inventor: Yuchen Zhou
  • MULTI-TARGET PHOTONIC BIOSENSOR, AND METHOD FOR MANUFACTURING AND PREPARING SAME
    Publication number: 20130316368
    Abstract: A component or device is provided for the detection or the measurement in parallel of one or more specific types of biological or chemical target products. This component includes a group of nanotubes selected and/or functionalized to interact with the target product, around an optical waveguide. Thus, an optical coupling is produced between the optical waveguide and one or more optical characteristics of these nanotubes, the modifications of which are evaluated in the presence of the target product. In addition, a method is provided for manufacturing and preparing such a component or device, and a detection method using them, as well as a post-manufacture preparation method comprising a specific functionalization for different target products starting from the same type of pluripotent generic component. Also provided is a family of PFO-based functionalization polymers.
    Type: Application
    Filed: February 9, 2012
    Publication date: November 28, 2013
    Applicants: UNIVERSITE PARIS SUD 11, COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Laurent Vivien, Etienne Gaufres, Nicolas Izard, Eric Doris, Edmond Gravel, Didier Boquet
  • Bioluminescent assays using cyanobenzothiazole compounds
    Patent number: 8592172
    Abstract: The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.
    Type: Grant
    Filed: March 11, 2011
    Date of Patent: November 26, 2013
    Assignee: Promega Corporation
    Inventors: Jessica Kelts, Poncho Meisenheimer, John Shultz, James J. Cali, Dongping Ma
  • FREE SOLUTION MEASUREMENT OF MOLECULAR INTERACTIONS BY BACKSCATTERING INTERFEROMETRY
    Publication number: 20130309661
    Abstract: Disclosed are methods, systems, and apparatuses for the free solution measurement of molecular interactions by backscattering interferometry. In one aspect, the invention relates to method and systems for detecting molecular interaction between analytes in free-solution wherein the analytes are label-free and detection is performed by back-scattering interferometry. Also disclosed are label-free, free-solution, and/or real-time measurements of characteristic properties and/or chemical events using the disclosed techniques. The disclosed methods can have very low detection limits and/or very low sample volume requirements. Also disclosed are various biosensor applications of the disclosed techniques. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
    Type: Application
    Filed: April 5, 2013
    Publication date: November 21, 2013
    Applicant: Vanderbilt University
    Inventor: Darryl J. Bornhop
  • METHODS AND REAGENTS FOR BIOMOLECULE LABELING, ENRICHMENT AND GENTLE ELUTION
    Publication number: 20130309689
    Abstract: Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications.
    Type: Application
    Filed: March 11, 2013
    Publication date: November 21, 2013
    Applicant: Pierce Biotechnology, Inc.
    Inventors: John Charles Rogers, Ryan Daniel Bomgarden, Christopher Laurence Etienne, Eric Leigh Hommema
  • TETRAMERIC ALPHA-SYNUCLEIN AS BIOMARKERS
    Publication number: 20130309690
    Abstract: The present invention provides the surprising finding that alpha-synuclein exists in vivo as a folded tetramer. Provided are various methods and technologies that arise from this finding, including methods and kits for identifying individuals susceptible to or suffering from certain diseases, disorders or conditions associated with stability of alpha-synuclein tetramers, and/or individuals likely (or not) to respond to therapy with agents that alter level and/or stability of alpha-synuclein tetramers.
    Type: Application
    Filed: November 4, 2011
    Publication date: November 21, 2013
    Applicants: THE BRIGHAM AND WOMEN'S HOSPITAL, INC., BRANDEIS UNIVERSITY
    Inventors: Dagmar Ringe, Gregory A. Petsko, Dennis J. Selkoe, Tim Bartels
  • Restricted access media and methods for making restricted access media
    Patent number: 8586318
    Abstract: The present invention is directed to restricted access media (RAM), methods for preparing restricted access media, and kits for preparing restricted access media that contain protected ligand binding agents or protected enzymes. Certain RAM provided contain a plurality of protected regions of the support that contain ligand binding agents that are protected by blocking agents. Certain RAM provided contain a plurality of protected regions of the support that contain unbound ligand binding agents or enzymes that are retained in the protected regions by a capping agent. Methods of making the RAM of the invention and associated kits are also provided.
    Type: Grant
    Filed: August 14, 2012
    Date of Patent: November 19, 2013
    Assignee: Board of Regents of the University of Nebraska
    Inventors: David S. Hage, Chunling Wa, Abby Jackson, Hai Xuan
  • Fluorescent protein particles
    Patent number: 8586315
    Abstract: A fluorescent protein particle comprising: a particle forming component capable of forming or aggregating into a substantially insoluble protein particle when expressed by a cell; a fluorescent component; and a functional component capable of binding to, or being bound by, a target.
    Type: Grant
    Filed: May 28, 2008
    Date of Patent: November 19, 2013
    Assignee: Innovative Purification Technologies Pty Ltd
    Inventors: Jens Sommer-Knudsen, David Moreland Gibbs
  • Recombinant ACE2 polypeptide dimer
    Patent number: 8586319
    Abstract: The present invention relates to recombinant ACE2 polypeptide, where the ACE2 polypeptide is present as a dimer. The dimer is formed specifically from glycosylated monomers and is used for producing pharmaceutical products with an extended half-life.
    Type: Grant
    Filed: June 12, 2008
    Date of Patent: November 19, 2013
    Assignee: Apeiron Biologics AG
    Inventors: Manfred Schuster, Hans Loibner, Evelyne Janzek-Hawlat, Bernhard Peball, Stefan Stranner, Bettina Wagner, Robert Weik
  • Method for enumeration of mammalian micronucleated erythrocyte populations, while distinguishing platelets and/or platelet-associated aggregates
    Patent number: 8586321
    Abstract: A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: November 19, 2013
    Assignee: Litron Laboratories, Ltd.
    Inventor: Stephen D. Dertinger
  • Methods of using GPR119 receptor to identify compounds which stimulate glucose-dependent insulinotropic peptide secretion
    Patent number: 8580526
    Abstract: The present invention relates to methods of using GPR119 receptor to identify compounds useful for increasing bone mass in an individual. Agonists of GPR119 receptor are useful as therapeutic agents for treating or preventing a condition characterized by low bone mass, such as osteoporosis, and for increasing bone mass in an individual. Agonists of GPR119 receptor promote bone formation in an individual.
    Type: Grant
    Filed: August 1, 2011
    Date of Patent: November 12, 2013
    Assignee: Arena Pharmaceuticals, Inc.
    Inventors: Zhi-Liang Chu, James N. Leonard
  • Methods for identifying compounds which modulate T2R bitter taste receptors
    Patent number: 8580527
    Abstract: The invention provides isolated nucleic acid and amino acid sequences of taste cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of taste cell specific G-protein coupled receptors.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: November 12, 2013
    Assignee: The Regents of the University of California
    Inventors: Charles S. Zuker, Jon E. Adler, Mark Hoon, Nick Ryba, Ken Mueller
  • Methods of screening for LTRPC7 modulators
    Patent number: 8580525
    Abstract: The present invention relates to the identification and isolation of a novel family of ATP regulated calcium transmembrane channel polypeptides designated herein as “LTRPC7” (Long Transient Receptor Potential Channel). Channels comprising these polypeptides close in response to concentrations of cytoplasmic ATP in the millimolar range, are subject to inhibition by high intracellular levels of calcium and/or magnesium, and do not respond to depletion or reduction in intracellular calcium stores. The invention further relates to the methods of utilizing LTRPC7 for binding, and the methods for modulating LTRPC7 activity and for measuring LTRPC2 permeability. The invention further relates to the methods of modulating expression of LTRPC7.
    Type: Grant
    Filed: May 28, 2008
    Date of Patent: November 12, 2013
    Assignee: The Queen's Medical Center
    Inventors: Reinhold Penner, Andrea Fleig
  • Methods for diagnosing pervasive development disorders, dysautonomia and other neurological conditions
    Patent number: 8580522
    Abstract: Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the individual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, Giardia, Cryptosporidium, E. histolytica, C. difficile, Adenovirus, Rotavirus or H. pylori.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: November 12, 2013
    Assignee: Curemark, LLC
    Inventor: Joan M. Fallon
  • Assays for the detection of anti-TNF drugs and autoantibodies
    Patent number: 8574855
    Abstract: The present invention provides assays for detecting and measuring the presence or level of anti-TNF? drug therapeutics and autoantibodies in a sample. The present invention is useful for optimizing therapy and monitoring patients receiving anti-TNF? drug therapeutics to detect the presence or level of autoantibodies (e.g., HACA and/or HAHA) against the drug.
    Type: Grant
    Filed: April 6, 2012
    Date of Patent: November 5, 2013
    Assignee: Nestec S.A.
    Inventors: Sharat Singh, Shui Long Wang, Linda Ohrmund
  • Immunoassay methods
    Patent number: 8574848
    Abstract: The invention generally relates to the field of diagnostic or prognostic assays and in particular relates to assays for the detection of antibodies in a sample comprising patient bodily fluid, wherein such antibodies are used as biological markers of a disease state or disease susceptibility. The assay is based on cross-titration of both the patient bodily fluid to be tested for the antibody and an antigen used to detect the antibody by specific binding.
    Type: Grant
    Filed: September 12, 2007
    Date of Patent: November 5, 2013
    Assignee: OncImmune Ltd.
    Inventors: John F. R. Robertson, Andrea Murray, Caroline Chapman, Anthony Barnes
  • Method and means for treatment of osteoarthritis
    Patent number: 8574857
    Abstract: The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inducing anabolic stimulation of chondrocytes, including cartilage formation enhancing pharmaceutical compositions, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean cartilage thickness in a subject.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: November 5, 2013
    Assignee: Galapagos N.V.
    Inventors: Nick Vandeghinste, Peter Herwig Maria Tomme, Frits Michiels, Libin Ma, Blandine Mille-Baker, Helmuth G. G. van Es
  • Modulators of angiogenesis and tumorigenesis
    Patent number: 8574827
    Abstract: The present invention relates to regulation of angiogenesis and tumorigenesis. The invention further relates to methods for identifying and using agents, including small organic molecules, antibodies, peptides, cyclic peptides, nucleic acids, antisense nucleic acids, RNAi, and ribozymes, that modulate angiogenesis via modulation of endothelial cell haptotaxis; as well as to the use of expression profiles and compositions in diagnosis and therapy of angiogenesis and cancer.
    Type: Grant
    Filed: October 29, 2003
    Date of Patent: November 5, 2013
    Assignee: Rigel Pharmaceuticals, Inc.
    Inventors: James B. Lorens, Robert E. Atchison, Annabelle Friera, Sacha Holland
  • Methods of detection of factor XIa and tissue factor
    Patent number: 8574849
    Abstract: The invention provides compositions and methods for the detection of Factor XIa or Tissue Factor (TF) activity in a sample using an antibody based clotting time prolongation assay. The invention provides methods for detection of FXIa or TF activity in a sample using a fluorogenic substrate. Further provided herein is a correlation between elevated levels of FXIa and/or TF with inflammation, acute coronary syndrome (ACS), myocardial infarction, coronary artery disease (CAD), heart failure, aortic stenosis, stroke, or transient ischemic attack. The frequency of FXIa and TF activity was substantially lower in individuals with stable coronary artery disease and no history of myocardial infarction. No FXIa or TF activity was observed in healthy individuals.
    Type: Grant
    Filed: October 3, 2008
    Date of Patent: November 5, 2013
    Assignee: The University of Vermont and State Agriculture College
    Inventors: Kenneth G. Mann, Saulius Butenas, Anetta Undas
  • Use of secretor, lewis and sialyl antigen levels in clinical samples as predictors of risk for disease
    Patent number: 8574850
    Abstract: An individual at risk for necrotizing enterocolitis and related disorders can be identified by measuring the level of at least one secretor antigen in a biological sample from the individual and comparing the measured level of the at least one secretor antigen to a predetermined value or a predetermined range of values. Among the secretor antigens which can be measured are: the H-1, H-2, Lewisb and Lewisy antigens and derivatives thereof (e.g., a sialylated form of Lewis a, Lewis x, Lewis b, Lewis y; H-1, H-2, Lewis a, Lewis x, Lewis b or Lewis y).
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: November 5, 2013
    Assignees: Instituto Nacional de Ciencias Medicas Y Nutricion, The General Hospital Corporation, Children's Hospital Medical Center
    Inventors: Ardythe L. Morrow, Guillermo Ruiz-Palacios, David S. Newburg
  • IMMUNOGENIC POLYPEPTIDES HAVING AN IMMUNOGENIC SCAFFOLD PROTEIN AND A LOOP PEPTIDE, PRESENTING A 3074- OR 2219/2557- MONOCLONAL ANTIBODY-TARGETED EPITOPE, WHICH IS PRESENT IN THE HIV GP120 PROTEIN
    Publication number: 20130287804
    Abstract: The present invention is directed to a recombinant immunogenic polypeptide. The polypeptide includes a loop peptide inserted into an immunogenic scaffold protein. The loop polypeptide has an amino acid sequence which presents the 3074 mAb- or the 2219/2557 mAb-targeted epitope of the HIV gp120 protein and not other known epitopes of the HIV gp120 protein. When used as an immunogen, the polypeptide induces an antibody response which neutralizes heterologous HIV-1 viruses in a pattern similar to that observed for the 3074 mAb- or the 2219/2557 mAb-targeted epitope, respectively. Pharmaceutical compositions containing the immunogenic polypeptide as well as methods of making and using it are also disclosed.
    Type: Application
    Filed: September 28, 2011
    Publication date: October 31, 2013
    Applicants: NEW YORK UNIVERSITY, UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY, UNIVERSITY OF MASSACHUSETTS, MOLSOFT LLC
    Inventors: Timothy Cardozo, Xian-peng Kong, Susan Zolla-Pazner, Abraham Pinter, Chavdar Krachmarov, Shan Lu, Shixia Wang, Maxim Totrov
  • SULFENIC ACID-REACTIVE COMPOUNDS AND THEIR METHODS OF SYNTHESIS AND USE IN DETECTION OR ISOLATION OF SULFENIC ACID-CONTAINING COMPOUNDS
    Publication number: 20130288268
    Abstract: The present invention provides compounds of Formula I: wherein: R1 is a label (e.g., a detectable groups; an anti-tumor agent)s; L is present or absent and when present is a linking group; and x represents an integer from 1 to 10; or a pharmaceutically acceptable salt thereof. the compounds are useful for, among other things, identifying cysteine sulfenic acids in proteins and monitoring oxidative damage in proteins and cells.
    Type: Application
    Filed: June 18, 2013
    Publication date: October 31, 2013
    Inventors: Leslie B. Poole, S. Bruce King, Jacquelyn S. Fetrow
  • System and method for controlling bacterial persister cells with weak electric currents
    Patent number: 8569027
    Abstract: A system and method for treating persister cells with an electrochemical process, alone or in combination with antibiotics. Weak electric currents are used to effectively eliminate persister cells and the efficacy can be further improved through synergistic effects with antibiotics. The method may be adapted for novel therapies of chronic infections and strategies to control persistent biofouling. The system has a broad spectrum applications in treating chronic and drug resistant infections, such as those caused by Pseudomonas aeruginosa, Mycobacterium tuberculosis and methicillin resistant Staphylococcus aureus, and may also be used for decontamination of medical devices.
    Type: Grant
    Filed: August 10, 2010
    Date of Patent: October 29, 2013
    Assignee: Syracuse University
    Inventors: Dacheng Ren, Mi Zhang, Tagbo Niepa, Jeremy Gilbert
  • Peptides and methods for the detection of Lyme disease antibodies
    Patent number: 8568989
    Abstract: The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to Borrelia antigens. The peptide compositions comprise polypeptide sequences comprising variants in the IR6 domain of the Borrelia VlsE protein. The invention also provides devices, methods, and kits comprising such peptide compositions and useful for the detection of antibodies that bind to Borrelia antigens and the diagnosis of Lyme disease.
    Type: Grant
    Filed: November 17, 2010
    Date of Patent: October 29, 2013
    Assignee: Abaxis, Inc.
    Inventors: Rajesh K. Mehra, Kenneth P. Aron, Dennis M. Bleile
  • Antibody categorization based on binding characteristics
    Patent number: 8568992
    Abstract: Methods for categorizing antibodies based on their epitope binding characteristics are described. Methods and systems for determining the epitope recognition properties of different antibodies are provided. Also provided are data analysis processes for clustering antibodies on the basis of their epitope recognition properties and for identifying antibodies having distinct epitope binding characteristics.
    Type: Grant
    Filed: May 24, 2012
    Date of Patent: October 29, 2013
    Assignee: Amgen Fremont Inc.
    Inventors: Wynn L. Walker, Michael L. Gallo, Xiao-Chi Jia, Keith Joho, Jaspal Singh Kang
  • Chemotaxis diagnostic
    Patent number: 8568987
    Abstract: A method is described for determining the chemotactic activity of leukocytes in a sample, in which method the expression of a cell adhesion molecule is determined.
    Type: Grant
    Filed: December 29, 2009
    Date of Patent: October 29, 2013
    Assignee: Glycotope Biotechnology GmbH
    Inventors: Carl Thomas Nebe, Karin Hartmann
  • Process for measuring antigen content
    Patent number: 8563256
    Abstract: A process for measuring the amount of an antigen in a sample comprising the steps of binding the antigen to a solid phase, forming an antigen-antibody immunocomplex on the solid phase by applying a detection antibody that is specific for the antigen, liberating the detection antibody from the immunocomplex by applying a polypeptide that disrupts the immunocomplex by competing against the antigen for binding to the detection antibody, collecting the liberated detection antibody; and quantifying the liberated detection antibody to measure the amount of the antigen in the sample.
    Type: Grant
    Filed: October 21, 2012
    Date of Patent: October 22, 2013
    Inventor: Jiandi Zhang
  • Sensitive and rapid methods of using chimeric receptors to identify autoimmune disease and assess disease severity
    Patent number: 8563257
    Abstract: The present invention provides methods and compositions useful in the diagnosis and management of autoimmune diseases. In particular, the present invention provides improved methods and compositions for the diagnosis and management of Graves' disease. The methods of the present invention not only avoids the need for radioactivity and are much simpler, economical, and rapid than methods traditionally used for the diagnosis of Graves' disease, but also improve upon the sensitivity and detection abilities of previous luciferase-based autoantibody detection assays. Such improvements are based upon the superior performance of assays comprising a chimeric TSH receptor in the presence of a glucocorticoid including, but not limited to, dexamethasone.
    Type: Grant
    Filed: June 12, 2009
    Date of Patent: October 22, 2013
    Assignee: Diagnostic Hybrids, Inc.
    Inventors: Leonard Kohn, Jim Brown, David Scholl, Yunsheng Li, Giorgio Napolitano
  • NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES
    Publication number: 20130273522
    Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.
    Type: Application
    Filed: March 28, 2013
    Publication date: October 17, 2013
    Inventors: THOMAS JAY LOWERY, JR., MARK JOHN AUDEH, JAMES FRANKLIN CHEPIN, Vasiliki Demas, Rahul K. Dhanda, MARILYN LEE FRITZEMEIER, MATTHEW BLANCO, ISAAC KOH, SONIA KUMAR, BRIAN M. MOZELESKI, LORI ANNE NEELY, DANIELLA LYNN PLOURDE, CHARLES WILLIAM RITTERSHAUS
  • LIPID ENCAPSULATION OF SURFACE ENHANCED RAMAN SCATTERING (SERS) NANOPARTICLES
    Publication number: 20130273561
    Abstract: Phospholipid-microvesicle-encapsulated surface enhanced Raman scattering (SERS) nanoparticles and methods for making the encapsulated particles are described. The encapsulated particles can be used in nanomedicine. Four Raman-active species were used. A bilayer was observed by TEM, and the SERS spectrum of each dye species (SERS reporter) was confirmed.
    Type: Application
    Filed: October 31, 2011
    Publication date: October 17, 2013
    Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: Gilbert C. Walker, Christina M. MacLaughlin, Shell IP
  • siRNA Against p22phox
    Publication number: 20130274312
    Abstract: The invention relates to siRNA against p22phox, compositions comprising the siRNA, methods of treating diseases with the siRNA and cell based systems for studying the effect of p22 phox modulation by siRNA or cells.
    Type: Application
    Filed: September 23, 2011
    Publication date: October 17, 2013
    Inventor: Donald R. Singer
  • Method for determining the toxicity of an environmental or chemical sample
    Patent number: 8551921
    Abstract: An indicator device, and a biological test method, for determining the toxic fingerprint and degree of toxicity, comprising at least 3, preferably at least 11, different microorganisms freeze-dried on an inert support material, wherein the microorganisms are being selected to form a high diversity of microorganisms, on the support material, with regards to the taxonomical tree and high diversity regarding responses to toxic chemicals. Further, a kit and a process for producing the indicator device is also disclosed.
    Type: Grant
    Filed: February 11, 2008
    Date of Patent: October 8, 2013
    Assignee: Phplate Stockholm AB
    Inventors: Inger Kuhn, Patricia Colque-Navarro, Jenny Gabrielson, Aina Iversen, John Douglas McKenzie, Mark Christopher Hart
  • Methods for screening cells and antibodies
    Patent number: 8551716
    Abstract: The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies.
    Type: Grant
    Filed: June 19, 2012
    Date of Patent: October 8, 2013
    Assignee: X-Body, Inc.
    Inventors: Christine C. Genick, Lance G. Laing, Peter Li, Timothy F. Smith, Lara Madison, Bo Lin
  • Bioluminescent detection of cyanohydroxy benzothiazole compounds
    Patent number: 8551721
    Abstract: The invention provides methods that employ derivatives of 2-cyano-6-hydroxy- or 2-cyano-6-amino-benzothiazole, for example, in a bioluminogenic reaction. Also provided are novel compounds that can be used in the methods. The invention further provides methods for detecting or determining the presence of molecules and/or enzymes, the modulator activity of such molecules, and/or the activity of such enzymes. The methods are adaptable to high-throughput format.
    Type: Grant
    Filed: September 9, 2009
    Date of Patent: October 8, 2013
    Assignee: Promega Corporation
    Inventors: Jessica Anderson, Poncho Meisenheimer, John Shultz, James J. Cali, Dongping Ma
  • Method for quantitative assessment of thymus integrity
    Patent number: 8551713
    Abstract: The present invention relates to a series of markers and a method of determining cause of death post-mortem by quantifying thymus integrity. An examination of thymuses from human infants suffering mortal head trauma revealed a disruption of the cortical-medullary organization of the thymus, particularly involving dissolution of the cortical-medullary border. A similar result was obtained for related mouse and rat models. The human thymuses from head trauma cases also displayed a higher percentage of Ki67-positive thymocytes.
    Type: Grant
    Filed: November 14, 2011
    Date of Patent: October 8, 2013
    Assignees: University of South Florida, H. Lee Moffitt Cancer Center and Research Institute, Inc.
    Inventors: George Blanck, Mark Lloyd
  • Isolation and use of ryanodine receptors
    Patent number: 8551694
    Abstract: The genes encoding ryanodine receptor homologs have been characterized from multiple insect families. The genes and their corresponding polypeptides have a number of uses including but not limited to the development of screens to identify insecticidally active compounds. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.
    Type: Grant
    Filed: January 10, 2012
    Date of Patent: October 8, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Steven Gutteridge, Timothy Caspar, Daniel Cordova, James J. Rauh, Yong Tao, Lihong Wu
  • Methods for identifying and isolating cells expressing a polypeptide
    Patent number: 8551715
    Abstract: The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies.
    Type: Grant
    Filed: February 11, 2011
    Date of Patent: October 8, 2013
    Assignee: OncoMed Pharmaceuticals, Inc.
    Inventors: Austin L. Gurney, Alexandra L. L. Lazetic, Christopher J. Bond
  • Monomeric and polymeric linkers useful for conjugating biological molecules and other substances
    Patent number: 8546309
    Abstract: The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: October 1, 2013
    Assignee: Dako Denmark A/S
    Inventor: Jesper Lohse
  • Tuberculosis vaccine with improved efficacy
    Patent number: 8545854
    Abstract: The present invention relates to novel recombinant vaccines providing protective immunity against tuberculosis. Further, the present invention refers to novel recombinant nucleic acid molecules, vectors containing said nucleic acid molecules, cells transformed with said nucleic acid molecules and polypeptides encoded by said nucleic acid molecules.
    Type: Grant
    Filed: June 22, 2011
    Date of Patent: October 1, 2013
    Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenshaften, e.V.
    Inventors: Leander Grode, Stefan H. E. Kaufmann, Baerbel Raupach, Juergen Hess
  • Detection method for methicillin resistant staphylococcus aureus
    Patent number: 8546093
    Abstract: The present invention relates to an antibody against a protein specifically expressed in methicillin-resistant strains of Staphylococcus aureus (MRSA), and a method and a kit for detecting MRSA. The present invention enables a fast and accurate detection of MRSA by using both a PBP2a-specific antibody for the detection of PBP2a and a Protein A-specific antibody for the detection of Protein A.
    Type: Grant
    Filed: June 24, 2009
    Date of Patent: October 1, 2013
    Assignee: Dinona Inc.
    Inventors: Hyung-Geun Song, Sang-Soon Yoon, Hae-Jung Kim, Gil-Yong Jee, Mi-Hyang Shin, Yu-Ri Moon
  • Potency test for vaccine formulations
    Patent number: 8546149
    Abstract: The invention relates to certain methods for the determination of an antigen content of a first antigen in a mixture comprising two or more antigens. The invention also relates to a potency test for an antigen in a combination vaccine. The method allows the determination of the antigen content in a mixture additionally comprising antibodies that are capable of binding with the antigen.
    Type: Grant
    Filed: September 3, 2010
    Date of Patent: October 1, 2013
    Assignees: Intervet Inc., Intervet International B.V.
    Inventors: Michelle Allen, Mark Garrett, Urs Peter Bruderer, Martinus Antonius Johannes Thijssen
  • Porphyrinic compounds for use in flow cytometry
    Patent number: 8546088
    Abstract: The present invention provides a method of detecting (e.g., by flow cytometry) a target compound, cell or particle, wherein the target is labelled with a detectable luminescent compound. The method comprises utilizing as the detectable luminescent compound a compound comprising a porphyrinic macrocycle such as a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin. In particular embodiments, the detectable luminescent compound comprises a compound of the formula A-A?-Z-B?-B, wherein: A is a targeting group or member of a specific binding pair that specifically binds the detectable luminescent compound to the target compound, cell or particle; A? is a linker group or covalent bond; B? is a linker group or covalent bond; B is a water-soluble group; and Z is the porphyrinic macrocycle.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: October 1, 2013
    Assignee: North Carolina State University
    Inventor: Jonathan S. Lindsey
  • High-throughput in vitro translation (cell-lysate based) assay for detecting quorum sensing signals
    Patent number: 8546071
    Abstract: A cell-lysate extract based assay reagent for detecting quorum sensing signals is generally provided, along with methods of making and using the same. The assay reagent generally includes a cell-lysate extract formed from a biosensor bacterium (e.g., Agrobacterium tumefaciens NTL4 (pCF218)(pCF372)) and a detecting substrate (e.g., an absorbance-based or luminescence-based substrate). The cell-lysate extract can be prepared by (1) disrupting the cell membranes of the biosensor bacterium to release the cellular components into a solution, (2) centrifuging the resulting solution, and (3) removing the resulting supernatant solution.
    Type: Grant
    Filed: July 28, 2008
    Date of Patent: October 1, 2013
    Assignee: University of South Carolina
    Inventors: Alan W. Decho, Tomohiro Kawaguchi, Yung-pin Chen
  • Sir2 activity
    Patent number: 8546074
    Abstract: Methods for screening a compound, by providing a test mixture comprising a transcription factor, Sir2, and a Sir2 cofactor with the compound, and evaluating an activity of a component of the test mixture in the presence of the compound are described.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: October 1, 2013
    Assignees: Massachusetts Institute of Technology, Whitehead Institute for Biomedical Research
    Inventors: Leonard P. Guarente, Homayoun Vaziri, Shin-Ichiro Imai
  • Device and method for identifying and determining blood groups
    Patent number: 8546084
    Abstract: A device for the determination and/or the detection of erythrocyte blood groups from a whole blood sample, and a process that implements this device, as well as a kit for the determination and the detection of blood groups are disclosed.
    Type: Grant
    Filed: July 2, 2008
    Date of Patent: October 1, 2013
    Assignee: ABO Diag
    Inventor: Najim Chaibi
  • FLUORESCENT COMPOUNDS
    Publication number: 20130251637
    Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.
    Type: Application
    Filed: December 20, 2011
    Publication date: September 26, 2013
    Applicants: DYOMICS GMBH, PIERCE BIOTECHNOLOGY, INC.
    Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann