Abstract: The synthesis of 10,10'-substituted-9,9'-biacridine molecules and their derivatives is disclosed. These molecules are shown to catalyze the production of light by chemiluminescence in the presence of a signal solution having at a pH from about 10.0 to about 14.0, at a concentration effective for producing a chemiluminescent signal, a chelating agent, a sulfoxide, a reducing sugar, an oxidant or combination of oxidants, an alcohol and aqueous sodium tetraborate. These 10,10'-substituted-9,9'-biacridines are used alone or attached to haptens or macromolecules and are utilized as labels in the preparation of chemiluminescent, homogeneous or heterogeneous assays. They are also used in conjunction with other chemiluminescent label molecules to produce multiple analyte chemiluminescent assays.

Abstract: Graphitic nanotubes, which include tubular fullerenes (commonly called "buckytubes") and fibrils, which are functionalized by chemical substitution, are used as solid supports in electrogenerated chemiluminescence assays. The graphitic nanotubes are chemically modified with functional group biomolecules prior to use in an assay. Association of electrochemiluminescent ruthenium complexes with the functional group biomolecule-modified nanotubes permits detection of molecules including nucleic acids, antigens, enzymes, and enzyme substrates by multiple formats.

Abstract: The invention concerns interference-eliminating agents for avoiding unspecific interactions in immunoassays in which avidin or streptavidin or a derivative thereof are used as the interference-eliminating agents.

Abstract: This invention relates to glycosyltransferase, genes encoding glycosyltransferase, recombinant vectors having such a gene, host cells transformed with such a recombinant vector, and uses thereof. This invention makes it possible to glycosylate indolopyrrolocarbazole derivatives conveniently and economically.

Abstract: The present invention provides a process for the determination of a specifically bindable substance by incubation of the sample solution with at least three receptors R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 and R.sub.2 are bindable with one another and R.sub.3 is specifically bindable with the substance to be determined and measurement of the agglutination which takes place in the case of the reaction, wherein, as receptor R.sub.1, there is used a conjugate of a partner of a specifically binding pair P and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, as receptor R.sub.2 there is used a receptor which has at least two binding positions for P and as receptor R.sub.3 there is used a receptor which has at least two binding positions, of which at least one binds specifically with an epitope of the substance to be determined or of S.

Abstract: The present invention provides analytical devices for the characterization of the primary structure of proteins and peptides, comprising a microenzyme reactor, a separation device, an interface between the microenzyme reactor and the separation device, a mass spectrometer, and an interface between the separation device and the mass spectrometer. Also provided are methods for characterizing a protein or peptide utilizing such devices.

Abstract: Synthesis of an amino-disaccharide, amino-oligosaccharide or a derivative thereof, characterized in that a monosaccharide, a disaccharide, an oligosaccharide, a glycoside or a derivative thereof, in the presence of a glycosidase as catalyst, is reacted with an amino-deoxy-saccharide or a derivative thereof, and that the amino-saccharide is isolated from the product mixture directly or after chemical/enzymatic modification.

Abstract: Coal is treated aerobically or anaerobically to produce humic acid, volatile fatty acids, lower alcohols, and/or methane using a consortium of bacteria designated Mic-1 or KSARC56. This process can also be used to convert aromatic compounds, such as phenols and derivatives thereof, to methane and carbon dioxide.

Abstract: Fluorogenic chelators for Eu.sup.3+ and Tb.sup.3+ are provided, they form highly fluorescent complexes with Eu.sup.3+ and Tb.sup.3+. In all cases, the fluorescence observed was lanthanide-specific, long-lived and it was monitored by microsecond time-resolved fluorometry. The fluorogenic chelators could be quantified, in the presence of excess lanthanide, at levels<10.sup.-8 mol/L. Fluorogenic chelators can form ternary complexes with Eu.sup.3+ and Tb.sup.3+, in the presence of EDTA. The structures of the identified chelators is such that enzyme substrates can be used for enzyme-labelled time-resolved fluorometric immunoassays.

Abstract: The present invention discloses novel immunogens, antibodies prepared from such immunogens, and labeled reagents useful in immunoassays for the detection and quantification of testosterone in a test sample. Also disclosed are immunoassays using these reagents and methods for synthesizing these reagents. The immunoassays are preferably microparticle enzyme immunoassays (MEIAs) and fluorescence polarization immunoassays (FPIAs). Further disclosed are novel starting materials for making the above novel immunogens and labeled reagents. Methods for making the novel immunogens and labeled reagents from the novel starting materials are also disclosed.

Abstract: The present invention provides a method for targeting boron atoms to tumor cells in a patient. The method comprises the steps of:(A) administering a targeting composition comprising a conjugate of a first member of a binding pair and an antibody, wherein the antibody selectively binds to antigens produced by or associated with the tumor cells, and allowing the conjugate to localize at said tumor cells;(B) optionally, administering a clearing composition, and allowing the clearing composition to clear non-localized conjugate from circulation;(C) administering a boron-containing compound comprising a conjugate comprising a complementary member of said binding pair and boron atoms, and allowing the compound to localize at the tumor cells. The method may further comprise the step of irradiating the boron atoms of the boron compound, thereby effecting BNCT of the tumor cells. Compositions and kits for carrying out the method also are provided.

Abstract: The invention describes useful conjugates of sulforhodamine, wherein the conjugated substance and the fluorophore are separated by an alkanoic acid spacer that is attached to the fluorophore via a sulfonamide bond. The increased length of the covalent linkage due to the spacer results in dye-conjugates having a number of surprisingly advantageous properties relative to previous sulforhodamine-labeled conjugates, including increased fluorescence.

Abstract: The present invention is directed to a method for selectively enhancing the production of factors A, and/or B.sub.0 of antibiotic A 40926 either to isolate these single components in better yields or to enrich the complex in one or both the above components, which comprises adding an appropriate precursor of the desired antibiotic factor to an A 40926 producing culture during fermentation.

Abstract: New A83543 components, including fermentation products A83543K, A835430, A83543P, A83543U, A83543V, A83543W and A83543Y and N-demethyl derivatives, and salts thereof, are useful for the control of insects and mites. The pseudoaglycones of the new A83543 components are useful for the preparation of A83543 components. Methods are provided for making the new A83543 components by culturing of Saccharopolyspora spinosa NRRL 18395, NRRL 18537, NRRL 18538, or NRRL 18539, or NRRL 18743 or NRRL 18719 or NRRL 18823 in suitable culture medium. Insecticidal and ectoparasiticidal compositions containing new A83543 components are also provided.

Abstract: A novel macrolactam monosaccharide isolated from an antimicrobial complex 517 produced in fermentation under controlled conditions using a biologically pure culture of the microorganism Actinomadura vulgaris subsp. vulgaris ATCC 53748.

Abstract: A species such as a microorganism, e.g. Legionella, Giardia or Cryptosporidium, is captured by first attracting plastic coated magneticbeads or other magnetically attractable particles to a solid support such as stainless steel mesh, which particles have a selective affinity for the species, e.g. by virtue of an antibody coating, and contacting a sample containing the species with the particles on the solid support. The beads bearing the captured species may be released by reduction of the magnetic attraction of the support for the beads, e.g. by turning off an electromagnet used to magnetize the support.

Abstract: The invention provides a method of detecting a class of substances, by providing an assay configuration which allows for reaction with a homologous portion common to all members of the class. The configuration utilizes a capture reactant to immobilize class members, revealing previously unrecognizable reaction sites on the class. The capture reactant interacts with a homologous portion on the class members. The immobilized class is then detected, such as by the use of a sandwich antibody directed to another homologous portion of the class now exposed for reaction, or directed to specific portions of specific members of the class that have been exposed for reaction. These reaction sites were unrecognizable prior to immobilization of the class members with the capture reactant.

Abstract: A method and system for separating a selected molecule from a heterogeneous mixture of molecules in aqueous solution are described. The method comprises (a) providing a separation device comprising a loading reservoir and a receiving reservoir coupled by a channel bearing immobilized microtubules aligned parallel to the longitudinal axis thereof the channel; (b) placing an aqueous solution containing the heterogeneous mixture of molecules in the loading reservoir; (c) adding a motor-ligand composition and ATP to the aqueous solution, wherein the motor-ligand composition comprises a motor protein for attaching to microtubules and moving therealong in the presence of ATP and the ligand is capable of binding the selected molecule, such that the ligand binds the selected molecule to form a complex and the complex moves along the immobilized microtubules to the receiving reservoir; and (d) removing the selected molecule from the receiving chamber.

Abstract: Disclosed are methods for converting isoflavone conjugates in vegetable protein materials to isoflavone aglucones by forming an aqueous mixture of a vegetable protein material containing isoflavone conjugates, converting the isoflavone conjugates to isoflavone glucones, and converting the isoflavone glucones to isoflavone aglucones. The vegetable protein material may also contain isoflavone glucones which are converted to isoflavone aglucones. The isoflavone conjugates, isoflavone glucones, or isoflavone aglucones present in the vegetable protein material may be removed from the vegetable materials before, during, or after the conversion process.

Abstract: A method to produce novel polyketide structures by designing and introducing specified changes in the DNA governing the synthesis of the polyketide is disclosed. The biosynthesis of specific polyketide analogs is accomplished by genetic manipulation of a polyketide-producing microorganism by isolating a polyketide biosynthetic gene-containing DNA sequence, identifying enzymatic activities associated within the DNA sequence, introducing one or more specified changes into the DNA sequence which codes for one of the enzymatic activities which results in an altered DNA sequence, introducing the altered DNA sequence into the polyketide-producing microorganism to replace the original sequence, growing a culture of the altered microorganism under conditions suitable for the formation of the specific polyketide analog, and isolating the specific polyketide analog from the culture.

Abstract: The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor b

Abstract: The invention relates to a process for immobilization onto the surface of enzyme linked immunosorbent assay (ELISA) plates of a compound or for immunization with a compound, wherein said compound is in the form of a compound carrier complex which is either an avidin-biotinyl compound complex or a streptavidin-biotinyl compound complex.

Abstract: Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a photoactive indicator precursor molecule to come into close proximity. The photosensitizer generates singlet oxygen which activates the photoactive indicator precursor to generate a photoactive indicator molecule. Upon irradiation with light the photoactive indicator molecule produces light, which is measured. The amount of light produced by the photoactive indicator is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.

Abstract: The invention concerns the use of a composition which is composed of several different antibodies or/and antibody fragments which serves as a reagent to eliminate rheumatoid factor interference in an immunochemical method.

Abstract: The present invention provides a method for quantifying the presence of extracellular LBP in body fluids including blood in a subject comprising conducting an LBP immunoassay on plasma obtained from said subject.

Abstract: Sites on antibodies having affinity for photoaffinity compounds, in particular purine or azidopurine containing compounds are taught. Such photoaffinity sites are contained on a conserved region of different antibody molecules. These sites provide for the site-specific attachment of nucleotide photoaffinity compounds to antibodies, e.g., ATP- or GTP-analog photoaffinity compounds by photochemical means. These nucleotide photoaffinity compounds may additionally be attached to molecules having a desired therapeutic or diagnostic activity, and the resultant conjugates used as diagnostics or therapeutics.

Abstract: The present invention relates to a method and assay device for detecting small analytes. The results of the assay can be directly read from the device, which is a lateral flow device.

Abstract: Assays and antibodies are disclosed for the detection and quantitation of cardiac specific troponin I and troponin T in body fluids as an indicator of myocardial infarction. Since troponin I and T exist in various conformations in the blood, the ratios of the monomeric troponin I and T and the binary and ternary complexes may be related to the metabolic state of the heart. More specifically, immunoassays for determining the presence or amount of free, binary and ternary complexes of troponin I and T are claimed.

Abstract: The application describes homogeneous assays for determining ligands. A sample is contacted with a labeled substance P.sub.2 and receptor R. The receptor R consists of one molecule of P.sub.1, which monovalently and specifically binds to P.sub.2, and one molecule for R.sub.1, which binds to the ligand of interest. Complexes of P.sub.1, P.sub.2, R.sub.1 and ligand form, and are determined. R.sub.1 must be a nucleic acid molecule.

Abstract: A test kit and method for the highly sensitive detection of specific analytes in a sample is provided. The presence of the analyte in the sample results in a decrease in the concentration of a growth inhibiting substance leading to proliferation of cells in the region of the analyte. The presence or absence of the analyte is determined by detecting the presence of increased numbers of cells. Assay sensitivity is accounted for by the exponential amplification of cell number that occurs during cell proliferation in the presence of analyte.

Abstract: A method of assaying for CAT in a fluid involves the use of a complex of chloramphenicol with a member of a specific binding pair such as a hapten or biotin. Biotinylated chloramphenicol is claimed as new. A scintillation proximity assay involves use of this reagent with tritiated acetyl coenzyme A and streptavidin coated SPA beads.

Abstract: This invention discloses novel immunoassay termed an ELONA, employing nucleic acid ligands as capture molecules and/or detector molecules.

Abstract: A method for substantially preventing coating of hydrophobic material on a probe of an automated analyzer during an assay is described. An automated analyzer having a probe is provided. A composition comprising a non-denaturing surfactant and a reagent for use in the assay is provided. The probe is contacted with the composition during the assay such that hydrophobic material is substantially prevented from coating the probe on the automated analyzer. Compositions for preventing such probe coating are also described. Methods and compositions comprising a non-denaturing surfactant for enhancing the stability of reagents used in an assay are also described.

Abstract: Novel antifungal antibiotics Cepacidine A (A.sub.1 and A.sub.2 ),a novel microorganism Pseudomonas cepacia AF 2001 producing the same, and a process for producing the said antibiotics are disclosed.

Abstract: This application describes a high throughput assay for screening for compounds capable of binding to a fusion protein which consists of a target protein and an FK506-binding protein. The method for preparing the DNA encoding for the fusion protein and for expressing that DNA is also described in the application. The invention also discloses the recombinant DNA and protein sequences for several fusion proteins.

Abstract: This application describes a high throughput assay for screening for compounds which are capable of binding to a fusion protein which consists of a target protein and an FKS506-binding protein.

Abstract: For the determination of an immunologically detectable substance based on a heterogeneous immunoassay by use of a solid phase on which one of the immunologically active reaction components is bound, a reaction vessel is used as the solid phase on the inner surface of which streptavidin or avidin is bound in such an amount that 0.1 to 2.5 .mu.g are present per ml reaction volume.A suitable reaction vessel for this has optically transparent wall areas which face one another and has avidin or streptavidin coated walls which are at least partially within the inner wall region intended as a receptacle for liquid, wherein the inner space of the container intended as a receptacle for liquid and the respective streptavidin or avidin content of the coating are so matched that 0.1 to 2.5 .mu.g streptavidin or avidin are present per ml reaction volume.

Abstract: Novel antifungal agents having the formula: ##STR1## wherein R is hydrogen or --C(O)--R.sub.1 wherein R.sub.1 is alkenyl, C.sub.2 -C.sub.12 -alkyl, aryl, arylalkenyl, arylalkyl, aryl-aryl-, arylalkoxy-aryl-, aryloxyl-aryl-, aryl-aryl-aryl- or arylalkoxy-aryl-aryl-, or pharmaceutically acceptable salts, esters or prodrugs thereof, as well as (i) pharmaceutical compositions comprising such compounds, (ii) methods of treatment using such compounds, and (iv) methods and fungal cultures useful in making the same.

Abstract: A particularly efficient design for a nonbibulous lateral flow one step assay for an analyte in a biological sample is disclosed. In the improved device of the invention, three zones which are in nonbibulous lateral flow contact are employed: a sample receiving zone, a labeling zone, and a capture zone. The sample containing analyte is carried through the labeling zone and interacts with an assay label comprising visible moieties, preferably particles, which are coupled to specific binding reagent for analyte or to a competitor with analyte for a capture reagent. The flow continues into the capture zone where the visible moieties to which analyte or competitor are coupled are captured. Excess fluid is absorbed into an absorbent zone in contact with the capture zone. A positive result is obtained by visualizing the visible moieties in the capture zone.

Abstract: Antimicrobial compounds having the formula ##STR1## wherein R.sup.1 and R.sup.2 are independently selected from the group consisting of hydrogen and C.sub.1 -to-C.sub.4 alkanoyl;R.sup.3 and R.sup.4 are selected from the group consisting of(a) R.sup.3 is hydrogen and R.sup.4 is hydroxy,(b) R.sup.3 is hydroxy and R.sup.4 is hydrogen,(c) R.sup.3 and R.sup.4 taken together are .dbd.O; or selected from the group consisting of hydrogen and hydroxy; andR.sup.5 and R.sup.6 are independently selected from the group consisting of hydrogen, bromine and chlorine, with the proviso that at least one of R.sup.5 and R.sup.6 must be bromine. Also disclosed are pharmaceutical compositions comprising such compounds, methods of treating bacterial infection by the administration thereof and a process for preparing said compounds.

Abstract: A mutated farnesyldiphosphate synthase capable of synthesizing geranylgeranyldiphosphate and gene coding for said mutated enzyme, wherein the mutated enzyme is modified from a native farnesyldiphosphate synthase by mutation of a gene coding for a native farnesyldiphosphate synthase.

Abstract: Particles comprising an energy donor as a first component and a fluorescent dye as a second component positioned in said particles at an energy exchanging distance from one another, wherein the two components have a Stokes shift of greater than or equal to 50 nm, said particle having bound on its surface, a protein, polypeptide, nucleic acid, nucleotide or protein containing ligand analogue are disclosed and claimed. In addition, novel fluorescent dyes are described which exhibit intramolecular energy transfer for use to label various molecules, proteins, polypeptides, nucleotides and nucleic acids or to incorporate into particles.

Abstract: The invention concerns a binding matrix containing a carrier material and a solid phase reactant which is adsorbed to this via anchor groups that is capable of binding to at least one free reaction partner wherein the solid phase reactant forms a dilute and essentially laterally homogeneous binding layer on the surface of the carrier material. In addition a method for the determination of an analyte in a sample solution is claimed in which a solid phase reactant is used which is a component of a binding matrix according to the present invention. In this process the specific binding reaction is preferably determined by optical reflection techniques.

Abstract: Desmethylbalhimycin, a compound of the formula C.sub.65 H.sub.71 Cl.sub.2 N.sub.9 O.sub.24, desmethylleucylbalhimycin, a compound of the formula C.sub.59 H.sub.60 Cl.sub.2 N.sub.8 O.sub.23, desglucobalhimycin, a compound of the formula C.sub.60 H.sub.63 Cl.sub.2 N.sub.9 O.sub.19, ureidobalhimycin, a compound of the formula C.sub.67 H.sub.74 Cl.sub.2 N.sub.10 O.sub.25, desmethyl-desglucobalhimycin, a compound of the formula C.sub.59 H.sub.61 Cl.sub.2 N.sub.9 O.sub.19 and balhimycin V, a compound of the formula C.sub.73 H.sub.84 Cl.sub.2 N.sub.10 I.sub.26, methylbalhimycin, a compound of the formula C.sub.67 H.sub.75 Cl.sub.2 N.sub.9 O.sub.24 and balhimycin R, a compound of the formula C.sub.72 H.sub.83 Cl.sub.2 N.sub.9 O.sub.28, have an antibiotic action.

Abstract: The invention provides novel methods and compositions for detecting kinase activity in solution, without the use of radioactivity. The methods marry the kinetic advantages of solution-based reaction with the efficiency and high-throughput adaptability of solid-phase wash and detection steps, yet is conveniently practiced in a single tube. The methods may be used to assay for kinase activity per se or, by controlling for the kinase activity, for modulators of kinase activity. The method is exemplified with a preferred chemiluminescent protein kinase assay using biotinylated substrate peptides captured on a streptavidin coated microtiter plate and monoclonal antibodies to detect their phosphorylation.

Abstract: The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.

Abstract: By using an assay device comprising a sample-receiving port, a pump-connection port, a sample-treating zone which is provided with a labeled substance with a label, a sample-treating and optical-measuring zone which has a porous material having immobilized thereto one of a specifically binding pair, and a pathway interconnecting these zones and ports, wherein the sample-treating zone and the sample-treating and optical-measuring zone are positioned between the sample-receiving port and the pump-connection port;or by using an assay device comprising a sample-receiving port, a pump-connection port, a sample-treating and optical-measuring zone which has a porous material having immobilized thereto a labeled substance with a label and one of a specifically binding pair, and a pathway interconnecting these zone and ports, wherein the sample-treating and optical-measuring zone is positioned between the sample-receiving port and the pump-connection port,biological components can be quantitatively determined by immun

Abstract: A test device for detecting or quantifying an analyte in a test sample includes an absorbent material having separate contact and measurement portions. The contact portion is positioned at or proximate to a first end of the absorbent material. The measurement portion has a receptor for a conjugate of an analyte analog and marker-encapsulating liposomes. In a method for using the test device, a binding material specific for the analyte is combined with the liposome-analyte analog conjugate and the test sample to form a test mixture. The mixture is incubated for a time sufficient to permit competition between any analyte present and the conjugate for the binding material. Following incubation, the mixture is allowed to traverse the absorbent material from the contact portion through the measurement portion of the absorbent material.

Abstract: An improved method for determining binding compounds from a mixture of similar compounds, particularly a phage peptide library, is provided. The method comprises contacting a mixture of candidate compounds with a target molecule presented on two or more different substrates.

Abstract: The present invention concerns a method for the determination of allele-specific peptide motifs on molecules of the major histocompatibility complex (MHC) of classes I and II as well as the peptide motifs which are obtainable by the method according to the invention. In addition the use of the peptide motifs according to the invention for the production of a diagnostic or therapeutic agent is disclosed.