Polynucleotide (e.g., Nucleic Acid, Oligonucleotide, Etc.) Patents (Class 435/91.1)
  • Patent number: 11834670
    Abstract: A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5? flanking sequence and a donor 3? flanking sequence. The donor 5? flanking sequence and the donor 3? flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: December 5, 2023
    Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLC
    Inventors: John Richard Nelson, Robert Scott Duthie, Patrick McCoy Spooner, John Anthony Schiel, Lisa Anne Lowery, Anja Josifa Smith
  • Patent number: 11814684
    Abstract: The present invention provides compositions and methods based on genetic polymorphisms that are associated with coronary heart disease (particularly myocardial infarction), aneurysm/dissection, and/or response to drug treatment, particularly statin treatment. For example, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by these nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and variant proteins, and methods of using the nucleic acid molecules and proteins as well as methods of using reagents for their detection.
    Type: Grant
    Filed: March 29, 2021
    Date of Patent: November 14, 2023
    Assignee: Celera Corporation
    Inventors: Olga Iakoubova, James J. Devlin, Carmen Tong, Charles Rowland
  • Patent number: 11767547
    Abstract: Methods and compositions for the amplification of nucleic acids and generation of concatemers are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases and primers.
    Type: Grant
    Filed: October 3, 2019
    Date of Patent: September 26, 2023
    Assignee: Labrador Diagnostics LLC
    Inventors: Kamila Belhocine, Josephine Lee, Pranav Patel, Aaron Richardson, Scott Tabakman
  • Patent number: 11746336
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing a 3-hydroxycarboxylic acid or ketone. The present invention also provides for a host cell comprising the PKS and when cultured produces the 3-hydroxycarboxylic acid or ketone.
    Type: Grant
    Filed: January 24, 2019
    Date of Patent: September 5, 2023
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Satoshi Yuzawa, Leonard Katz, Jay D. Keasling
  • Patent number: 11661628
    Abstract: The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: May 30, 2023
    Assignee: Takara Bio USA, Inc.
    Inventors: Emmanuel Kamberov, Tong Sun, Eric Bruening, Jonathon H. Pinter, Irina Sleptsova, Takao Kurihara, Vladimir L. Makarov
  • Patent number: 11639505
    Abstract: Described herein are UV-resistant or UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing UV-resistant proteins such as, for example, hexokinase, heat shock proteins, alcohol dehydrogenase, transferrin, flavonol synthase, zinc oxidase, and iron oxidase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: May 2, 2023
    Assignee: Bio Capital Holdings, LLC
    Inventor: Raul Cuero Rengifo
  • Patent number: 11634754
    Abstract: Disclosed is a hybridisation capture method based on the pyrophosphorolysis reaction. According to the present invention, there is provided a method for increasing the ratio of a first nucleic add sequence to second nucleic add sequence in a sample.
    Type: Grant
    Filed: April 15, 2022
    Date of Patent: April 25, 2023
    Inventors: Robert Osborne, Magdalena Stolarek-Januszkiewicz, Barnaby Balmforth
  • Patent number: 11634758
    Abstract: A nucleic acid amplification and detection apparatus, including: a support configured to receive a plurality of reaction vessels containing respective samples of one or more nucleic acids to be amplified, the support being rotatable about an axis of rotation and the reaction vessels being received in the support at respective receiving locations distributed about the axis of rotation; a temperature control component thermally coupled to the support and configured to control the temperature of the support in order to amplify the nucleic acids contained in the reaction vessels while received in the support; one or more measurement components configured to measure one or more characteristics of the nucleic acids within the reaction vessels at respective measurement locations distributed about the axis of rotation; an actuator coupled to the support and configured to rotate the support about the axis of rotation; and a sample position controller coupled to the actuator and being configured to rotate the support a
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: April 25, 2023
    Assignee: AXXIN PTY LTD
    Inventor: William Robb Hopper
  • Patent number: 11555219
    Abstract: The present application provides methods for detecting a nucleic acid molecule involving the use of a signal code sequence which corresponds to said nucleic acid molecule and a plurality of labelled detection probes which yield signals which make up the signal code sequence. In particular, the invention provides a sequential barcoding and decoding scheme which utilises a sequencing-by-hybridisation (SBH) strategy to sequence and decode a nucleotide barcode sequence, and to differentiate the nucleotide barcode sequence from other nucleotide barcode sequences. In an extension of the method, the application also provides a new coding scheme for providing a target nucleic acid with a detectable “colour” (or similar signal)-based code.
    Type: Grant
    Filed: June 1, 2021
    Date of Patent: January 17, 2023
    Assignee: 10x Genomics, Inc.
    Inventors: Malte Kühnemund, Toon Verheyen
  • Patent number: 11542500
    Abstract: The present invention relates to compositions and methods for promoting the degradation of misfolded proteins and protein aggregates. The compositions and methods may be used to treat a disorder associated with misfolded proteins or protein aggregates. In certain instances, the compositions and methods relate to modulators of one or more TRIM proteins or one or more STUbLs.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: January 3, 2023
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Xiaolu Yang, Lili Guo
  • Patent number: 11535892
    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g.
    Type: Grant
    Filed: July 15, 2021
    Date of Patent: December 27, 2022
    Assignee: Element Biosciences, Inc.
    Inventors: Sinan Arslan, Junhua Zhao, Molly He, Samantha Snow, William Light, Matthew Kellinger, Michael Previte, Michael Kim, Hua Yu, Yu-Hsien Hwang-Fu, Marco Tjioe, Andrew Boddicker
  • Patent number: 11453855
    Abstract: Large scale processes for producing high purity samples of biologically active molecules of interest from bacterial cells are disclosed. The methods comprise the steps of producing a lysate solution by contacting a cell suspension of said plurality of cells with lysis solution; neutralizing said lysate solution with a neutralizing solution to produce a dispersion that comprises neutralized lysate solution and debris; filtering the dispersion through at least one filter; performing ion exchange separation on said neutralized lysate solution to produce an ion exchange eluate; and performing hydrophobic interaction separation on said ion exchange eluate to produce a hydrophobic interaction solution. Further, provided are compositions comprising large scale amounts of plasmid DNA produced by the disclosed large scale processes.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: September 27, 2022
    Assignee: VGXI, INC.
    Inventors: Ruxandra Draghia-Akli, Henry Hebel, Ying Cai
  • Patent number: 11414695
    Abstract: A method of enriching for a fragment of a genome, as well as corresponding compositions and kits, are provided. In certain embodiments, the method comprises: (a) contacting a sample comprising fragmented DNA with a Cas9-gRNA complex comprising mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in the DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented DNA; and (b) isolating the complex. In addition, other methods and compositions for Cas9/CRISPR-mediated nucleic acid manipulation are also provided.
    Type: Grant
    Filed: May 29, 2014
    Date of Patent: August 16, 2022
    Assignee: Agilent Technologies, Inc.
    Inventors: Brian Jon Peter, Robert A. Ach
  • Patent number: 11372144
    Abstract: An interference filter includes a layers stack comprising a plurality of layers of at least: layers of amorphous hydrogenated silicon with added nitrogen (a-Si:H,N) and layers of one or more dielectric materials, such as SiO2, SiOx, SiOxNy, a dielectric material with a higher refractive index in the range 1.9 to 2.7 inclusive, or so forth. The interference filter is designed to have a passband center wavelength in the range 750-1000 nm inclusive. Added nitrogen in the a-Si:H,N layers provides improved transmission in the passband without a large decrease in refractive index observed in a-Si:H with comparable transmission. Layers of a dielectric material with a higher refractive index in the range 1.9 to 2.7 inclusive provide a smaller angle shift compared with a similar interference filter using SiO2 as the low index layers.
    Type: Grant
    Filed: March 19, 2019
    Date of Patent: June 28, 2022
    Assignee: MATERION CORPORATION
    Inventors: Robert Sprague, Shengyuan Bai
  • Patent number: 11260387
    Abstract: Methods of determining methylation of DNA are provided.
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: March 1, 2022
    Assignee: CEPHEID
    Inventors: Edwin Wei-Lung Lai, Andrew Kohlway, Reuel Van Atta, Russell Higuchi, Alexander A. Gall, Kriszten Kocmond
  • Patent number: 11254929
    Abstract: A method for one-pot one-step assembly of two or more DNA molecules to form at least one recombinant DNA molecule, and a substrate and a kit for this purpose. A simple and cost-effective assembly method for DNA molecules. A method for one-pot one-step assembly of two or more DNA molecules to form at least one recombinant DNA molecule is provided, wherein the two or more DNA molecules to be assembled are brought together in dry form with a suitable reaction medium on at least one substrate present in a reaction vessel.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: February 22, 2022
    Assignee: Leibniz-Institut fuer Pflanzenbiochemie (IPB)
    Inventor: Sylvestre Marillonnet
  • Patent number: 11236401
    Abstract: The present invention relates to a cell which is genetically modified with respect to its wild type and which comprises a gene sequence coding for a fluorescent protein, wherein the expression of the fluorescent protein depends on the amount of protein that is secreted across the cytoplasmic membrane into the extracytosolic space.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: February 1, 2022
    Assignee: SenseUp GmbH
    Inventors: Sarah-Kristin Jurischka, Georg Schaumann, Stephan Binder, Britta Kleine, Roland Freudl
  • Patent number: 11226348
    Abstract: A storage module for a laboratory automation system, a method of operating a laboratory automation system, and a laboratory automation system are presented. Items used by laboratory stations are stored centrally in a storage module and can be transported to the laboratory stations using a laboratory sample distribution system.
    Type: Grant
    Filed: December 15, 2017
    Date of Patent: January 18, 2022
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Urs Vollenweider, Gottlieb Schacher, Goran Savatic, Marco Maetzler, Christoph Ludwig, Christian Loewenstein, Yves Laloux, Rik Harbers, Matthias Edelmann, Andreas Drechsler
  • Patent number: 11193098
    Abstract: A PCR apparatus comprises a PCR heating block having at least two heater units, wherein the at least two heater units are repeatedly disposed on one side of a substrate in a first direction, and each of the at least two heater units has two or more heaters; and a PCR chip having at least two reaction chambers, wherein the at least two reaction chambers are repeatedly formed in the PCR chip, and when the PCR chip is in contact with the PCR heating block, the at least two reaction chambers are arranged to be contacted with the at least two heater units on the PCR heating block, wherein the PCR chip is repeatedly moved in a back-and-forth direction parallel to the first direction and the at least two reaction chambers of the PCR chip is placed to be in contact with the at least two heater units of the PCR heating block.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: December 7, 2021
    Assignee: NANOBIOSYS INC.
    Inventors: Sung Woo Kim, Jae Young Byun, Eun-Sub Kim, Duck Joong Kim
  • Patent number: 11168363
    Abstract: The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: November 9, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Graham Hall, Gavin Harper, Andrew John Heron, James White
  • Patent number: 11136618
    Abstract: Described herein are nucleic acid molecules and complexes useful as i-switch pH reporters that have increased sensitivities as a pH reporter and have alternate pH reporting capacity ranges. Aspects of the disclosure relate to a method for determining pH comprising providing a nucleic acid complex comprising: a first single-stranded nucleic acid molecule comprising the sequence CnXCnYCnZCn (SEQ ID NO.
    Type: Grant
    Filed: May 18, 2016
    Date of Patent: October 5, 2021
    Assignees: The University of Chicago, The National Centre for Biological Sciences, Tata Institute of Fundamental Research
    Inventors: Yamuna Krishnan, Saheli Halder
  • Patent number: 11111500
    Abstract: Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant.
    Type: Grant
    Filed: July 23, 2018
    Date of Patent: September 7, 2021
    Assignees: CIBUS US LLC, CIBUS Europe B.V.
    Inventors: Gregory F. W. Gocal, Peter R. Beetham, Aura Estela Gonzalez Schopke, Sarah Dumm, James Pearce, Christian Schopke, Keith A. Walker
  • Patent number: 11104943
    Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
    Type: Grant
    Filed: October 22, 2018
    Date of Patent: August 31, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Arkadiusz Bibillo, Pranav Patel
  • Patent number: 11104935
    Abstract: Kits for detecting analyte polynucleotides and an internal control in a sample. Included in the kit are an internal control polynucleotide and amplification reagents to co-amplify a first analyte polynucleotide and the internal control. Also included are first and second hybridization probes, each having a label indistinguishable from the other. The probes are respectively capable of hybridizing with a first analyte amplicon and an internal control amplicon. The first and second labels are indistinguishable homogeneous labels.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: August 31, 2021
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Sunghae A. Joo, Janel M. Dockter
  • Patent number: 11085073
    Abstract: The present invention is directed to a method for immobilizing a nucleic acid molecule on a solid support and to a use of a combination of a first nucleic acid immobilized primer linked to a solid support and a second immobilized primer linked to said solid support in said method.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: August 10, 2021
    Assignee: QIAGEN GmbH
    Inventors: Christian Korfhage, Evelyn Fricke
  • Patent number: 10989724
    Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
    Type: Grant
    Filed: July 31, 2017
    Date of Patent: April 27, 2021
    Assignee: Labrador Diagnostics LLC
    Inventors: Elizabeth A. Holmes, Daniel Young, Samartha Anekal, Timothy Smith, James R. Wasson, Chinmay Pangarkar
  • Patent number: 10988501
    Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: April 27, 2021
    Inventors: Handong Li, Snezana Drmanac, Radoje Drmanac, Xun Xu, Lingling Peng, Scott Gablenz
  • Patent number: 10968451
    Abstract: Nucleic acid molecules such as shRNA clusters and artificial miRNA clusters are disclosed, Also disclosed are methods of use, compositions, cells, viral particles, and kits relating to the nucleic acid molecules disclosed herein. The disclosure provides, at least in part nucleic acid molecules such as shRNA clusters encoding shRNA-like molecules and artificial miRNA clusters encoding modified pri-miRNA-like molecules. The shRNA clusters and artificial miRNA clusters disclosed herein can be used, for example, to produce artificial RNA molecules, e.g., RNAi molecules. Cells, viral particles, compositions (e.g., pharmaceutical compositions), kits, and methods relating to the nucleic acid molecules, e.g., shRNA clusters and artificial miRNA clusters, are also disclosed. The nucleic acid molecules (e.g., shRNA clusters and artificial miRNA clusters), artificial RNA molecules (e.g., RNAi molecules), cells, viral particles, compositions (e.g.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: April 6, 2021
    Assignee: Texas Tech University System
    Inventors: Haoquan Wu, Jang-gi Choi
  • Patent number: 10953100
    Abstract: The invention relates to novel compounds with the ability to link an immune response to a defined therapeutic target, to the use of said compounds in treating cancer and a disease or disorder mediated and/or caused by an infective agent, to compositions containing said compounds, processes for their preparation and to novel intermediates used in said process.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: March 23, 2021
    Assignee: CENTUARI THERAPEUTICS LIMITED (GB/GB)
    Inventors: Christopher Pickford, Christine Watson, Melanie Glossop
  • Patent number: 10947545
    Abstract: The present invention relates to new aptamer molecules for use in therapy of a subject by inhibiting or suppressing the activation of TLR9 in a cell, a method of inhibiting or suppressing the activation of TLR9 in a cell using such aptamer molecules, a pharmaceutical composition and a kit comprising such aptamer molecules and the use of aptamer molecules for inhibiting or suppressing TLR9 activation.
    Type: Grant
    Filed: October 27, 2017
    Date of Patent: March 16, 2021
    Assignee: Berlin Cures GmbH
    Inventors: Annekathrin Haberland, Katrin Wenzel
  • Patent number: 10934547
    Abstract: Disclosed are compositions and methods related to RNA interference (RNAi) and the use of RNAi active sequence for treating diseases and disorders. Particular disclosed are toxic RNAi active sequences such as siRNA and shRNA for killing cancer cells. The disclosed toxic RNAi active sequences typically include trinucleotide repeats and preferentially target the expression of multiple essential genes for cell survival and/or growth.
    Type: Grant
    Filed: February 20, 2018
    Date of Patent: March 2, 2021
    Assignee: Northwestern University
    Inventors: Marcus E. Peter, Andrea E. Murmann
  • Patent number: 10934569
    Abstract: This invention relates to processes that transcribe DNA molecules containing non-standard nucleotides using variants of T7 RNA polymerase to give RNA transcripts that contain their complementary non-standard nucleotides. Non-standard nucleotides pair during transcription using patterns of hydrogen bonding that are different from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: March 2, 2021
    Inventors: Nicole A Leal, Steven A Benner
  • Patent number: 10927140
    Abstract: Methods for reverse automated nucleic acid synthesis, and 5?-H-phosphonates suitable for use in the same, as well as methods for making 5?-H-phosphonates, are described.
    Type: Grant
    Filed: April 15, 2019
    Date of Patent: February 23, 2021
    Assignee: The University of Toledo
    Inventors: Matthew Ellis, Amanda Bryant-Friedrich
  • Patent number: 10920226
    Abstract: Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for LDHA.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: February 16, 2021
    Assignee: Thermo Fisher Scientific Inc.
    Inventors: Anastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Steven Read, Stephen Scaringe
  • Patent number: 10881743
    Abstract: Disclosed herein are polynucleic acid molecules, pharmaceutical compositions, and methods for treating muscle atrophy or myotonic dystrophy.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: January 5, 2021
    Assignee: AVIDITY BIOSCIENCES, INC.
    Inventors: Andrew John Geall, Venkata Ramana Doppalapudi, David Sai-Ho Chu, Michael Caramian Cochran, Michael Hood, Beatrice Diana Darimont, Rob Burke, Yunyu Shi, Gulin Erdogan Marelius, Barbora Malecova
  • Patent number: 10870872
    Abstract: The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3? terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3? terminal nucleotide such that the selected nucleotide becomes a 3? terminal nucleotide, and repeating the addition step until the polynucleotide is formed.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: December 22, 2020
    Assignee: President and Fellows of Harvard College
    Inventors: Henry Hung-yi Lee, George M. Church, Reza Kalhor
  • Patent number: 10851138
    Abstract: Disclosed are methods related to the generation and use of cellular and humoral responses for the prevention and treatment of P. gingivalis related conditions and diseases, such as methods of preparing P. gingivalis antibodies, comprising immunizing a non-human animal with a chimeric or fusion protein, wherein the protein comprises a first peptide joined directly or through a linker to a second peptide or polypeptide, wherein (A) the first peptide comprises a region of a P. gingivalis trypsin-like enzyme and (B) the second peptide or polypeptide comprises an adhesin domain of P. gingivalis.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: December 1, 2020
    Assignee: ORAL HEALTH AUSTRALIA PTY LTD
    Inventors: Eric Charles Reynolds, Neil Martin O'Brien Simpson, Keith J Cross, Nada Slakeski
  • Patent number: 10801058
    Abstract: Disclosed is a method of quality control of nucleic acid amplification using quality control oligonucleotide. The method comprises a nucleic acid detection step and a determination step. The nucleic acid detection step comprises the steps of: preparing a nucleic acid sample containing a target nucleic acid and a quality control polynucleotide; preparing a compartment containing one molecule of the target nucleic acid and a compartment containing one molecule of the quality control polynucleotide; and carrying out nucleic acid amplification of the target nucleic acid and the quality control polynucleotide, in the compartments, and carrying out signal detection using a detection probe to detect a signal originated from the detection probe. In the determination step, it is determined as to whether or not the nucleic acid detection step is proper on the basis of the result obtained in the signal detection step.
    Type: Grant
    Filed: January 13, 2017
    Date of Patent: October 13, 2020
    Assignee: SYSMEX CORPORATION
    Inventors: Tsuyoshi Nakano, Yuichiro Yoshida
  • Patent number: 10738306
    Abstract: The invention relates to an artificial nucleic acid molecule comprising at least one 5?UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and/or a 3?UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: August 11, 2020
    Assignee: CureVac AG
    Inventor: Andreas Thess
  • Patent number: 10704084
    Abstract: The present disclosure is directed to methods and kits for identifying, enriching, and evaluating templated assembly reactants. Some embodiments disclose methods for identifying templated assembly targets by synthesizing templated assembly reactants, hybridizing the templated assembly reactants to target nucleic acids, performing a templated assembly reaction, and identifying the target nucleic acids that hybridized to the templated assembly reactants. Libraries of templated assembly reactants, a kit for identifying templated assembly targets, and a pair of templated assembly targets enriched from a library of chemically-ligated oligonucleotides spatially elicited (CLOSE) products are also disclosed.
    Type: Grant
    Filed: December 2, 2015
    Date of Patent: July 7, 2020
    Assignee: TriBiotica LLC
    Inventors: Ian Dunn, Matthew Lawler
  • Patent number: 10688174
    Abstract: The present invention relates to vaccine compositions comprising Norovirus antigens and adjuvants, in particular, mixtures of monovalent VLPs and mixtures of multivalent VLPs, and to methods of conferring protective immunity to Norovirus infections in a human subject.
    Type: Grant
    Filed: June 3, 2019
    Date of Patent: June 23, 2020
    Assignee: Takeda Vaccines, Inc.
    Inventors: Charles Richardson, Thomas S. Vedvick, Thomas R. Foubert, William Tino
  • Patent number: 10639609
    Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: May 5, 2020
    Assignee: TWIST BIOSCIENCE CORPORATION
    Inventors: William Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle
  • Patent number: 10618024
    Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: April 14, 2020
    Assignee: TWIST BIOSCIENCE CORPORATION
    Inventors: William Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle
  • Patent number: 10576446
    Abstract: Provided herein is a method for capturing DNA molecules in solution. The method may comprise: extracting DNA from a sample that comprises endogenous DNA and environmental DNA to produce extracted DNA; ligating universal adaptors to the extracted DNA; hybridizing the extracted DNA, in solution, with affinity-tagged RNA probes generated by: in vitro transcribing a library of fragmented reference genomic DNA that has been ligated to an RNA promoter adaptor, in the presence of an affinity-tagged ribonucleotide; binding the product with a capture agent that is tethered to a substrate in the presence of RNA oligonucleotides that are complementary to the adaptors, thereby capturing the hybridized DNA molecules on the substrate; washing the substrate to remove any unbound DNA molecules; and releasing the captured DNA molecules. A kit for performing the method is also provided.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: March 3, 2020
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Carlos D. Bustamante, Meredith L. Carpenter, Jason D. Buenrostro, William J. Greenleaf
  • Patent number: 10564118
    Abstract: Methods for detecting polynucleotides, especially the DNA replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodeficiency virus, by detecting electromagnetic signals (“EMS”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“PCR”).
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: February 18, 2020
    Inventor: Luc Montagnier
  • Patent number: 10538762
    Abstract: Provided herein are compositions and methods for reducing expression of C9orf72 transcripts in cells containing expanded intronic GGGGCC regions, including those in subjects having or at risk of developing amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Provided herein are a double-stranded oligonucleotides of 13 to 22 nucleobases in length targeting a GGGGCC expanded repeat region in an intron of C9orf72, comprises (a) 3-5 central mismatches (within bases 9-14) within a target sequence comprising the expanded repeat sequence, or (h) 3-5 mismatches outside of the seed sequence (bases 2-8 within the guide strand complementary to the expanded repeat sequence).
    Type: Grant
    Filed: October 8, 2015
    Date of Patent: January 21, 2020
    Assignee: The Board of Regents of the University of Texas System
    Inventors: David Corey, Jiaxin Hu
  • Patent number: 10538795
    Abstract: The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5? protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5? protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, and optionally modifying the extension product of said nucleic acids without 5? protecting group either on the 5? end of the template strand or on the 3? end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified, wherein therefore the labelled nucleic acid d
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: January 21, 2020
    Assignee: LEXOGEN GMBH
    Inventors: Alexander Seitz, Irmlind Gabler, Lukas Paul
  • Patent number: 10519498
    Abstract: The invention relates to methods, compositions, devices, systems and kits are described including, without limitation, reagents and mixtures, for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: December 31, 2019
    Assignee: Qiagen Sciences, LLC
    Inventor: James J. DiMeo
  • Patent number: 10487353
    Abstract: Reaction mixture containing an analyte probe and an internal control probe, where the analyte probe and the internal control probe target different nucleic acids and have labels that generate signals that are indistinguishable from each other.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: November 26, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Sunghae A. Joo, Janel M. Dockter
  • Patent number: 10465184
    Abstract: The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
    Type: Grant
    Filed: September 15, 2015
    Date of Patent: November 5, 2019
    Assignee: BIONEER CORPORATION
    Inventors: Han Oh Park, Jae Ha Kim, Jong Gwang Park