Acellular Exponential Or Geometric Amplification (e.g., Pcr, Etc.) Patents (Class 435/91.2)
  • Patent number: 10767208
    Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.
    Type: Grant
    Filed: September 6, 2012
    Date of Patent: September 8, 2020
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Jijumon Chelliserry, Steven T. Brentano, Dmitry Lyakhov, Matthew C. Friedenberg, Anne-Laure Shapiro
  • Patent number: 10752905
    Abstract: This document provides methods and materials for assembling nucleic acid constructs (e.g., TALENs). For example, methods for assembling TALEs that are rapid, flexible for use in many cloning scaffolds (such as common nuclease and nickase backbones), and achievable with standard molecular biology laboratory tools, thereby making TALEs a more accessible genome system, are provided.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: August 25, 2020
    Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
    Inventors: Stephen C. Ekker, Karl J. Clark, Jarryd M. Campbell, Chun Hang Alvin Ma
  • Patent number: 10752947
    Abstract: Apparatus and methods to identify nucleotides of a DNA strand. The method includes exposing the DNA strand to a first dye or peptide, attaching the first dye or peptide to a first type of nucleotide (A,T,C,G) of the DNA strand, the first dye or peptide changing a conductance of the first type of nucleotide to which the first dye or peptide is attached, and measuring a tunneling current signal for all nucleotides of the DNA strand, the changed conductance of the first type of nucleotide providing amplified tunneling current discrimination of the nucleotides of the DNA strand.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: August 25, 2020
    Assignee: SEAGATE TECHNOLOGY LLC
    Inventors: Thomas Young Chang, Philip L. Steiner, Kim Yang Lee, David S. Kuo
  • Patent number: 10752944
    Abstract: The invention provides methods of forming a circular template for sequencing a target nucleic acid. The circular template is generated by amplification of a segment of the target nucleic acid with chimeric primers with complementary 5? ends. The circular template has a single nick or gap providing a site for initiation of template-directed extension for sequence analysis. Sequencing of a single template generates reads of alternating segments of the same strand of the target nucleic spaced by primer segments. The different reads of the same strand of the target nucleic acid can be compiled to generate a consensus sequence. Because only one strand of the target nucleic acid is sequenced per reaction, the present method avoids errors introduced by unwittingly combining sequences of both strands of a heteroduplex PCR product.
    Type: Grant
    Filed: September 6, 2012
    Date of Patent: August 25, 2020
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Matthew C. Friedenberg, Anne-Laure Shapiro
  • Patent number: 10738357
    Abstract: Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided.
    Type: Grant
    Filed: May 21, 2019
    Date of Patent: August 11, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Paul Giresi, Jason D. Buenrostro, Howard Y. Chang, William J. Greenleaf
  • Patent number: 10731152
    Abstract: A composition and method for controlled in vitro fragmentation of nucleic acids. A transposase forms catalytically active complexes with a modified transposon end that contains within its end sequence degenerate, apurinic/apyrimidinic sites, nicks, or nucleotide gaps, to fragment or shear a target nucleic acid sample in a controlled process. This method yields desired average nucleic acid fragment sizes. The inventive composition and method may be applied for generation of DNA fragments containing shortened transposon end sequences to facilitate subsequent reactions, for production of asymmetrically tailed DNA fragments, etc.
    Type: Grant
    Filed: November 17, 2015
    Date of Patent: August 4, 2020
    Assignee: THERMO FISHER SCIENTIFIC BALTICS UAB
    Inventors: Mindaugas Ukanis, Arvydas Lubys, Romas Tamo{hacek over (s)}evicius, Ervinas Gaidamauskas
  • Patent number: 10724085
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: February 22, 2018
    Date of Patent: July 28, 2020
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Patent number: 10710069
    Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: July 14, 2020
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Gene Parunak, Cecelia Haley
  • Patent number: 10710046
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
    Type: Grant
    Filed: June 18, 2018
    Date of Patent: July 14, 2020
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, John Milton, Geoffrey Paul Smith, Colin Lloyd Barnes, Isabelle Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu
  • Patent number: 10689681
    Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
    Type: Grant
    Filed: June 2, 2014
    Date of Patent: June 23, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Viktor Marius Boer, Erwin Suir
  • Patent number: 10655169
    Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: May 19, 2020
    Assignees: University of Utah Research Foundation, Biofire Defense, LLC
    Inventors: Luming Zhou, Carl T. Wittwer, Philip Seth Bernard, Virginie Dujols
  • Patent number: 10633698
    Abstract: Disclosed are a composition for PCR including polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), the composition for PCR being capable of increasing the efficiency and specificity of PCR and shortening PCR time, and a PCR method using the same.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: April 28, 2020
    Assignee: KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP
    Inventors: Dong Eun Kim, Hyo Ryoung Kim, Ah Ruem Baek
  • Patent number: 10620123
    Abstract: The disclosure relates to a fluorescence detection instrument, including a base, a heating module, a detecting module, an illumination module, and an actuation module. The heating module, the detecting module, and the actuation module are disposed on the base. The heating module includes plural heating holders, wherein each of the plural heating holders is adapted to accommodate a light-transmissive reaction container adapted to contain a fluorescent reaction mixture with at least one targeted fluorescent probe respectively. The detecting module is configured with the heating module to form plural detection channels, wherein the plural heating holders are located at the plural detection channels respectively. The actuation module is connected with the illumination module and adapted to drive the illumination module to move to at least one predetermined position to selectively match at least one combination of the heating holder on the corresponding detection channel.
    Type: Grant
    Filed: March 1, 2018
    Date of Patent: April 14, 2020
    Assignee: DELTA ELECTRONICS INT'L (SINGAPORE) PTE LTD
    Inventors: Bo Ma, Jei-Yin Yiu, Song-Bin Huang, Po-Yao Huang, Chun-Jung Li, Yu-Kai Kao
  • Patent number: 10612101
    Abstract: Methods for the rapid detection of the presence or absence of Mycoplasma genitalium (MG) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the target MG gene, along with kits are provided that are designed for the detection of MG.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: April 7, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Jody Harris, Kalyani Mangipudi
  • Patent number: 10612854
    Abstract: A sample holder for annealing apparatus and electrically assisted annealing apparatus using the same are provided. The sample holder includes a heat conductive shell, high thermal conductive and electrical insulation blocks, first and second electrodes. The heat conductive shell includes a base frame and a top cover. The high thermal conductive and electrical insulation blocks are adjacent to the base frame and the top cover, respectively, and a sample pallet is sandwiched therebetween. Length and width of the sample pallet is smaller than that of the high thermal conductive and electrical insulation blocks. The first and the second electrodes are fixed to two sides of the sample pallet, and are connected to electrifying wire respectively. Thickness of the first and the second electrodes is smaller than that of the sample pallet, while the width of the first and the second electrodes is longer than that of the sample pallet.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: April 7, 2020
    Assignee: Industrial Technology Research Institute
    Inventors: Hsu-Shen Chu, Chien-Neng Liao, Yao-Hsiang Chen
  • Patent number: 10604798
    Abstract: A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: March 31, 2020
    Inventors: Philip Roche, Andrew Kirk, Lenore Beitel, Miltiadis Paliouras, Mark Trifiro, Vamsy Chodavarapu, Mohamed Najih, Joachim Thiemann
  • Patent number: 10604787
    Abstract: A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium. The first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: March 31, 2020
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Gregory W. Sitton, Neil Percy
  • Patent number: 10597723
    Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.
    Type: Grant
    Filed: February 27, 2019
    Date of Patent: March 24, 2020
    Assignee: Natera, Inc.
    Inventors: Joshua Babiarz, Tudor Pompiliu Constantin, Lane A. Eubank, George Gemelos, Matthew Micah Hill, Huseyin Eser Kirkizlar, Matthew Rabinowitz, Onur Sakarya, Styrmir Sigurjonsson, Bernhard Zimmermann
  • Patent number: 10591502
    Abstract: A cassette may include a substrate, a die coupled to the substrate, a reservoir defined in a first side of the substrate exposing a proximal side of the die to an external atmosphere, and an overflow reservoir defined in the first side of the substrate and surrounding at least a portion of a perimeter of the reservoir.
    Type: Grant
    Filed: April 3, 2017
    Date of Patent: March 17, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Gary G. Lutnesky, Dennis R. Esterberg
  • Patent number: 10584319
    Abstract: The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black® RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is ˜200-fold over background, which is considerably better than other donor-quencher pairs (STNRs ˜10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.
    Type: Grant
    Filed: October 10, 2016
    Date of Patent: March 10, 2020
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: John Chaput, Andrew Larsen, Matthew Dunn
  • Patent number: 10577417
    Abstract: The present invention provides compositions and methods for regulating the specificity and activity of immune effector cells for use in immunotherapy. In one embodiment, the invention provides a type of chimeric antigen receptor (CAR) wherein the CAR is termed a “NKR-CAR” which is a CAR design comprising a component of a receptor naturally found on natural killer (NK) cells. In one embodiment, the NK receptor includes but is not limited to a naturally occurring activating and inhibitory receptor of NK cells known as a killer cell immunoglobulin-like receptor (KIR).
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: March 3, 2020
    Assignees: Novartis AG, The Trustees of the University of Pennsylvania
    Inventors: Gregory Beatty, Boris Engels, Neeraja Idamakanti, Carl H. June, Andreas Loew, Michael C. Milone, Huijuan Song, Enxiu Wang, Qilong Wu
  • Patent number: 10563251
    Abstract: First and second sets of receptacles containing reagents for first and second amplification reactions, respectively, are placed in first and second receptacle holders, each associated with a thermal element. The receptacles are subjected to different first and second incubation processes resulting in the first and second amplification reactions in each of the first and second sets of receptacles that contain a first or a second target nucleic acid, respectively. The presence or absence of the first or second target nucleic acid, if any, is determined for each receptacle of the first and second sets of receptacles.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: February 18, 2020
    Assignee: Gen-Probe Incorporated
    Inventors: David Buse, David Howard Combs, Norbert D. Hagen, David Opalsky, Bruce Richardson, Anita Prasad, Keith Moravick, Tyler Moore
  • Patent number: 10563254
    Abstract: Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.
    Type: Grant
    Filed: January 23, 2008
    Date of Patent: February 18, 2020
    Assignee: CAMBRIDGE ENTERPRISE LIMITED
    Inventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
  • Patent number: 10557158
    Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: February 11, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Paul Hardenbol, Pranav Patel, Benjamin Hindson, Paul William Wyatt, Keith Bjornson, Indira Wu, Kamila Belhocine
  • Patent number: 10538806
    Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
    Type: Grant
    Filed: February 4, 2019
    Date of Patent: January 21, 2020
    Assignee: KEYGENE N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Adrianus Johannes Van Tunen
  • Patent number: 10516670
    Abstract: Sharing data is disclosed. In some cases, sharing data includes receiving a request to share data from a first account to a second account, receiving an indication of a plurality of first account profiles associated with the first account to share with the second account, and establishing sharing from the plurality of first account profiles to the second account, wherein sharing comprises the second account having read access to a subset of nonpublic data associated with the plurality of first account profiles.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: December 24, 2019
    Assignee: 23andMe, Inc.
    Inventors: Brian Lee Hawthorne, Oleksiy Khomenko, Jeffrey Mellen, Marcela Miyazawa, Michael Polcari, Jack Tihon, Alexander Wong, Anne Wojcicki, Linda Avey
  • Patent number: 10500230
    Abstract: Isolated or purified mammalian kidney-derived cell populations from mammalian kidney tissue are provided. Methods are provided for the isolation and purification of the mammalian kidney-derived cell population. Methods for treating kidney disease are provided by administration of the isolated or purified mammalian kidney-derived cell population to a mammalian subject.
    Type: Grant
    Filed: October 11, 2007
    Date of Patent: December 10, 2019
    Assignee: DePuy Synthes Products, Inc.
    Inventors: David C. Colter, Agnieszka Seyda, Charito S. Buensuceso, Anna Gosiewska
  • Patent number: 10501783
    Abstract: The present invention relates to methods and uses for the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules in a sample during quantitative real-time polymerase chain reaction (qPCR) amplification. According to the invention, an intercalating dye recognizing double-stranded DNA molecules with higher affinity than single-stranded DNA molecules and a fluorophore-labeled oligonucleotide-probe being sequence specific for a target nucleic acid molecule are simultaneously employed, thus enabling quantification a specific target and total amount of a mixed nucleic acid population, and enabling assessing the cause of suboptimal PCR performance.
    Type: Grant
    Filed: December 9, 2014
    Date of Patent: December 10, 2019
    Assignee: QIAGEN GMBH
    Inventors: Nan Fang, Andreas Missel, Katja Heitz, Holger Wedler
  • Patent number: 10435740
    Abstract: The subject invention pertains to a method of determining methylation state and chromatin structure of target loci. The method comprises treating the genetic material obtained from the cells with DNA methyltransferase, capturing target genetic loci using a set of oligonucleotides, ligating the target loci with oligonucleotide patches that flank the target loci, treating the target loci flanked by oligonucleotide patches with bisulfite, optionally amplifying the target loci by polymerase chain reaction, sequencing the PCR products, and analyzing the sequences to determine methylation state and chromatin structure of the target loci. The current invention also provides a method to identify genes associated with a disease. The invention also provides a method to detect cells suffering from a disease in a group of cells. The current invention also provides kits suitable for carrying out the method of determining methylation state and chromatin structure of the target loci.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: October 8, 2019
    Assignee: University of Florida Research Foundation, Incorporated
    Inventors: Michael P. Kladde, Nancy H. Nabilsi, Carolina E. Pardo
  • Patent number: 10435759
    Abstract: The present invention disclosed a Nosema bombycis HMG1 gene, a specific primer set used for rapidly detecting Nosema bombycis, a group of microsporidium universal detection primers, and uses thereof. The primer set comprises a primer HMG1-sF and a primer HMG1-sR, and nucleotide sequences of the primers are shown in SEQ ID No. 5-6 respectively. The universal detection primers comprise a primer HMG1F and a primer HMG1R, and nucleotide sequences of the primers are shown in SEQ ID No. 3-4 respectively.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: October 8, 2019
    Assignee: SOUTH CHINA AGRICULTURAL UNIVERSITY
    Inventors: Jiping Liu, Xiaojing Song, Wei Cheng
  • Patent number: 10428363
    Abstract: Methods for amplifying nucleic acids are provided. The methods can be used to minimize sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimizing GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilizing flow cells.
    Type: Grant
    Filed: October 17, 2016
    Date of Patent: October 1, 2019
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Jonathan Mark Boutell, Min-Jui Richard Shen
  • Patent number: 10432640
    Abstract: Sharing data is disclosed. In some cases, sharing data includes receiving a request to share data from a first account to a second account, receiving an indication of a plurality of first account profiles associated with the first account to share with the second account, and establishing sharing from the plurality of first account profiles to the second account, wherein sharing comprises the second account having read access to a subset of nonpublic data associated with the plurality of first account profiles.
    Type: Grant
    Filed: May 22, 2019
    Date of Patent: October 1, 2019
    Assignee: 23andMe, Inc.
    Inventors: Brian Lee Hawthorne, Oleksiy Khomenko, Jeffrey Mellen, Marcela Miyazawa, Michael Polcari, Jack Tihon, Alexander Wong, Anne Wojcicki, Linda Avey
  • Patent number: 10415097
    Abstract: The present invention provides methods for the detection of cyanobacteria, and in particular, methods for the detection of cyanotoxic organisms. The invention further relates to methods of screening for compounds that modulate the activity of polynucleotides and/or polypeptides of the saxitoxin biosynthetic pathways.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: September 17, 2019
    Assignee: Newsouth Innovations PTY Limited
    Inventors: Brett A. Neilan, Troco Kaan Mihali, Ralf Kellmann, Young Jae Jeon
  • Patent number: 10416168
    Abstract: The invention concerns a method of analyzing the content of drops, involving then following step: providing a plurality of drops (6) contained in a carrier fluid, at least one of the drops (6) comprising at least one aggregate (10) of particles defining an object extending along a main axis, at least some of the drops (6) containing at least one target element capable of attaching to the aggregate (10). The method involves a step in which a physical parameter characteristic of the attachment of the target element to the aggregate (10) is measured.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: September 17, 2019
    Assignee: ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS
    Inventors: Andrew David Griffiths, Raphaël Clément Li-Ming Doineau, Clément Nizak, Philippe Chi-Thanh Nghe, Jean Marie Pierre Baudry, Elodie Michéle Christine Brient-Litzler, Alexei Godina
  • Patent number: 10392614
    Abstract: Provided herein are methods for immune repertoire sequencing and single cell barcoding. In some aspects, such methods may comprise producing a plurality of cDNAs from a plurality of polynucleotides, wherein at least one polynucleotide encodes a heavy and/or light chain, from a plurality of immune cells from a biological sample. Producing a plurality of cDNAs may comprise reverse transcription and template switching, such as to form a plurality of uniquely barcoded cDNAs. Methods described herein further comprise amplifying the plurality of uniquely barcoded cDNAs, thereby forming a library, and sequencing one or more of the sequences of the library.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: August 27, 2019
    Assignee: AbVitro LLC
    Inventors: Francois Vigneault, Adrian Wrangham Briggs
  • Patent number: 10385404
    Abstract: This invention relates to a kit for detecting NRAS gene mutation, and this kit can be used to detect cancer-related NRAS gene mutation. The said kit comprises: (1) the internal reference detection reagent, which includes the internal reference gene specific primers, internal reference gene specific probes and dNTP solution; (2) the NRAS mutation detection reagent, which includes the NRAS gene mutant type specific primers, NRAS gene mutant type specific probes, internal control gene specific primers, internal control gene specific probes and dNTP solution; (3) the Taq DNA polymerase; and (4) the NRAS positive quality control.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: August 20, 2019
    Assignee: Jiaxing ACCB Diagnositc Ltd.
    Inventors: Minli Mo, Hui Li, Zhao Chen, Feng Ding, Jun Li
  • Patent number: 10370702
    Abstract: The present invention relates to methods of making linear nucleic acid probes using rolling circle amplification methods.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: August 6, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Chao-ting Wu, Brian Beliveau
  • Patent number: 10358642
    Abstract: Disclosed is a method of preparing nucleic acid molecules, including: providing a pool of oligonucleotides, each containing restriction enzyme digestion sequences and generic flanking sequences; cleaving the restriction enzyme digestion sequence portions to provide a pool of mixtures comprising the oligonucleotides, each containing the generic flanking sequences at one end, and the oligonucleotides, each containing none of the generic flanking sequences at one end, and; assembling the oligonucleotides using the generic flanking sequences to randomly synthesize nucleic acid fragments.
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: July 23, 2019
    Assignee: CELEMICS, INC.
    Inventors: Duhee Bang, Hwangbeom Kim, Hyojun Han
  • Patent number: 10351848
    Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: July 16, 2019
    Assignee: MGI TECH CO., LTD.
    Inventors: Chunyu Geng, Ruoying Chen, Yuan Jiang, Xia Zhao, Rongrong Guo, Lingyu He, Yaqiao Li, Wenwei Zhang, Hui Jiang, Radoje Drmanac
  • Patent number: 10329544
    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components.
    Type: Grant
    Filed: April 6, 2016
    Date of Patent: June 25, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Chieh-Yuan Li, David Ruff, Shiaw-Min Chen, Jennifer O'Neil, Rachel Kasinskas, Jonathan Rothberg, Bin Li, Kai Qin Lao, Wolfgang Hinz
  • Patent number: 10330694
    Abstract: There is provided an automated biological-sample-processing system comprising a pipette, a column of solid-phase material to which nucleic acid binds, a transport apparatus, an air-piston apparatus and an adaptor for coupling the pipette to the transport apparatus and to the air-piston apparatus, in which the adaptor is removably engageable with the transport apparatus and the air-piston apparatus for movement with the transport apparatus during processing of the sample, is couplable to the pipette so that the transport apparatus is controllable to position the pipette and so that the air-piston apparatus is controllable to draw a liquid into the pipette and to expel the liquid from the pipette, and is engageable with the column, in which the adaptor comprises a filter for preventing liquid or aerosol transfer between the pipette or column and the air-piston apparatus.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: June 25, 2019
    Assignee: DIAGNOSTICS FOR THE REAL WORLD, LTD
    Inventors: Philip Stankus, Paul Duesbury, Torbjorn Blad, Craig Wisniewski, Jean-Pierre Allain
  • Patent number: 10323273
    Abstract: The present invention relates to methods and uses where a combination of (i) an enzyme exhibiting 5??3? exonuclease activity and specifically degrading 5?-phosphorylated strands of double-stranded nucleic acids is used, and (ii) one or more primer pair(s), each primer being 5? phosphorylated, in a method comprising at least a first and a second amplification reaction of a first and a second template, respectively, for preventing contamination of the second amplification reaction with amplification products of the first amplification reaction, as well as novel methods and kits using the combination. Also, the present invention relates to kits comprising the enzyme and either 5?-phosphorylated primers or a polynucleotide kinase.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: June 18, 2019
    Assignee: Qiagen GmbH
    Inventors: Nan Fang, Dirk Loeffert, Christine Runde
  • Patent number: 10308978
    Abstract: Transposon nucleic acids comprising a transposon end sequence and a calibration sequence for DNA sequencing in the transposon end sequence. In one embodiment, the transposon end sequence is a Mu transposon end. A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with the calibration sequence providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates.
    Type: Grant
    Filed: October 24, 2017
    Date of Patent: June 4, 2019
    Assignee: THERMO FISHER SCIENTIFIC BALTICS UAB
    Inventors: Ian Kavanagh, Laura-Leena Kiiskinen, Heli Haakana
  • Patent number: 10287636
    Abstract: A universally usable method for specific detection of target nucleic acid sequences, which method can be performed very rapidly and also simply and furthermore which does not need any expensive instrumental systems. The method is intended to be suitable as a molecular genetic rapid test and to respect the requirements of diagnostic specificity assurance. In this regard it is important that only one specific amplification product be detected and that amplification artifacts can be unambiguously discriminated. A nucleic acid amplification kit suitable for performing this method.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: May 14, 2019
    Assignee: AJ INNUSCREEN GmbH
    Inventors: Timo Hillebrand, Elmara Graser
  • Patent number: 10262103
    Abstract: Methods to formulate treatments for individual cancer patients by assessing genomic and/or phenotypic differences between cancer and normal tissues and integrating the results to identify dysfunctional pathways are described.
    Type: Grant
    Filed: November 18, 2009
    Date of Patent: April 16, 2019
    Inventors: Raphael Lehrer, Robert Coopersmith
  • Patent number: 10253358
    Abstract: The present invention provides DNA plasmids that find use as calibrators or reference standards for calculating DNA quantities in a sample. In particular, provided herein are DNA plasmids that contain multiple control fragments, and methods of their use in DNA quantitation.
    Type: Grant
    Filed: November 4, 2014
    Date of Patent: April 9, 2019
    Assignee: EXACT SCIENCES DEVELOPMENT COMPANY, LLC
    Inventors: Michael Domanico, Ilse A. Tyler, Brian Aizenstein, Hatim Allawi, Graham P. Lidgard
  • Patent number: 10246702
    Abstract: Provided herein are methods and compositions for labeling target nucleic acid molecules with molecular barcodes.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: April 2, 2019
    Assignee: New England Biolabs, Inc.
    Inventors: Cynthia L. Richard, Brendan Galvin
  • Patent number: 10246701
    Abstract: The invention relates to methods and compositions for estimating the absolute abundance individually for each unique rearranged lymphocyte receptor in a mixed sample.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: April 2, 2019
    Assignee: ADAPTIVE BIOTECHNOLOGIES CORP.
    Inventors: William S Dewitt, Ryan O Emerson, Harlan S Robins, Anna M Sherwood
  • Patent number: 10240146
    Abstract: The present invention generally relates to systems and methods for producing nucleic acids. In some aspects, relatively large quantities of oligonucleotides can be produced, and in some cases, the oligonucleotides may have a variety of different sequences and/or lengths. For instance, a relatively small quantity of oligonucleotides may be amplified to produce a large amount of nucleotides. In one set of embodiments, oligonucleotides may be amplified using PCR, then transcribed to produce RNA. The RNA may then be reverse transcribed to produce DNA, and optionally, the RNA may be selectively degraded or removed, relative to the DNA. In one set of embodiments, the oligonucleotides may be chemically modified. These modifications may include, but are not limited, to the adding of fluorescent dyes or other signaling entities.
    Type: Grant
    Filed: July 29, 2015
    Date of Patent: March 26, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaowei Zhuang, Jeffrey R. Moffitt, Alistair Boettiger
  • Patent number: 10239059
    Abstract: In one aspect, a thermal cycler system is disclosed. The thermal cycler can be comprised of a device housing and a cover that is operably connected to the device housing. The cover can include a handle portion, a device lid portion, a sample block platen, and a link bar. The device lid portion is attached to the proximal side of the handle portion with a first pin. The sample block platen is operably connected to the handle portion such that the sample block platen is positioned against the sample block when the handle portion is flush with the device lid portion and the cover is in a closed position. The link bar is pivotably connected to the device housing at a first terminal end portion and a second pin at an opposite second terminal end portion, wherein the handle portion is elevated away from the device lid portion before the cover is moved to an open position.
    Type: Grant
    Filed: February 14, 2014
    Date of Patent: March 26, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Tiong Han Toh, Wuh Ken Loh, Kuan Moon Boo, Sandro Klein, Daniel Welsh