Acellular Exponential Or Geometric Amplification (e.g., Pcr, Etc.) Patents (Class 435/91.2)
  • Patent number: 11459610
    Abstract: The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by exonuclease treatment and optionally blocking the 3? ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: October 4, 2022
    Assignee: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Michael Chesney, Vincent Peter Smith, Claire Bevis-Mott, Jonathan Mark Boutell, Angela Kalbande
  • Patent number: 11447821
    Abstract: Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.
    Type: Grant
    Filed: January 24, 2020
    Date of Patent: September 20, 2022
    Assignee: CAMBRIDGE ENTERPRISE LIMITED
    Inventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
  • Patent number: 11434540
    Abstract: Ultraspecific, programmable nucleic acid sensors capable of detecting and preferentially amplifying target DNA molecules comprising a particular SNP or mutation are provided. In some cases, the ultraspecific programmable nucleic acid sensors are useful for detecting SNP-containing DNA molecules indicative of cancer such as cell-free DNA circulating in the blood or indicative of organ transplant rejection Also provided are methods for construction of such ultraspecific nucleic acid sensors and methods for preferential amplification of target DNA molecules containing a mutation of interest, as well as testing systems for early cancer screening and routine monitoring of circulating cancer DNA using liquid biological samples such as serum, plasma, or saliva.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: September 6, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Alexander Green, Fan Hong, Soma Chaudhary, Anli Tang, Hao Yan
  • Patent number: 11427863
    Abstract: The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction.
    Type: Grant
    Filed: August 9, 2011
    Date of Patent: August 30, 2022
    Assignee: Envirologix Inc.
    Inventor: Stephen A. Judice
  • Patent number: 11421259
    Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
    Type: Grant
    Filed: July 20, 2018
    Date of Patent: August 23, 2022
    Assignee: Global Life Sciences Solutions Operations UK Ltd
    Inventors: John Richard Nelson, Robert Scott Duthie, Erik Leeming Kvam, Wei Gao
  • Patent number: 11421238
    Abstract: The present invention relates to a method for introducing mutations into at least one target nucleic acid molecule comprising (a) providing at least one sample comprising at least one target nucleic acid molecule; and (b) amplifying the at least one target nucleic acid molecule using a low bias DNA polymerase. The present further relates to a use of a low bias DNA polymerase in a method for introducing mutations into one or more nucleic acid molecule(s), a group of sample tags, a method for designing the group of sample tags, a computer readable medium, and a method for preferentially amplifying target nucleic acid molecules.
    Type: Grant
    Filed: February 19, 2019
    Date of Patent: August 23, 2022
    Assignee: LONGAS TECHNOLOGIES PTY LTD
    Inventors: Leigh G. Monahan, Joyce To, Catherine M. Burke, Michael Imelfort, Aaron E. Darling
  • Patent number: 11371087
    Abstract: The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid amplification conditions.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: June 28, 2022
    Assignee: Takara Bio USA, Inc.
    Inventors: Thomas D. Schaal, Jude Menlo Dunne, Maithreyan Srinivasan, Alain Mir
  • Patent number: 11352663
    Abstract: The invention provides a method and kit for preventing inhibition of a thermal cycling reaction by protein coagulation in a sample.
    Type: Grant
    Filed: March 16, 2018
    Date of Patent: June 7, 2022
    Assignee: Genedrive Diagnostics Ltd
    Inventors: Shaun Ainsworth, Ben Cobb, Gino Miele
  • Patent number: 11344892
    Abstract: The present disclosure provides a digital PCR system. The system includes a droplet formation assembly and a droplet orifice assembly. The droplet formation assembly includes a heat conducting plate and a cover plate, at least one inverted U-shaped step is placed on a side surface of the cover plate, the heat conducting plate, the cover plate and the inverted U-shaped step together form a droplet formation chamber having an opening at a bottom. The droplet orifice assembly is connected below the droplet formation assembly, and includes a plurality of droplet orifices, the droplet orifice is connected with the droplet formation chamber, and a vaporization component is placed in the droplet orifice, the vaporization component vaporizes a digital PCR solution in the droplet orifice and rapidly pushes the digital PCR solution into a droplet forming oil in the droplet formation chamber, to form a digital PCR droplet.
    Type: Grant
    Filed: November 22, 2018
    Date of Patent: May 31, 2022
    Assignee: Shanghai Industrial μTechnology Research Institute
    Inventors: Xuanye Wu, Yimin Guan
  • Patent number: 11299511
    Abstract: The present disclosure provides a solid phase method of making oligonucleotides via sequential coupling cycles including at least one coupling of a dinucleotide dimer subunit to a free 3?-terminal group of a growing chain. The oligonucleotides include at least two nucleoside subunits joined by a N3??P5? phosphoramidate linkage. The method may include the steps of (a) deprotecting the protected 3? amino group of a terminal nucleoside attached to a solid phase support, said deprotecting forming a free 3? amino group; (b) contacting the free 3? amino group with a 3?-protected amino-dinucleotide-5?-phosphoramidite dimer in the presence of a nucleophilic catalyst to form an internucleoside N3??P5? phosphoramidite linkage; and (c) oxidizing (e.g., sulfurizing) the linkage. The compositions produced by the subject methods may include a reduced amount of one or more (N?x) oligonucleotide products. Also provided are pharmaceutical compositions including the subject oligonucleotide compositions.
    Type: Grant
    Filed: September 14, 2017
    Date of Patent: April 12, 2022
    Assignee: Geron Corporation
    Inventor: Premchandran H. Ramiya
  • Patent number: 11299718
    Abstract: The present invention is directed to methods for replicating, amplifying, and sequencing of nucleic acids using the thermostable, bifunctional replicase “TthPrimPol” from Thermus thermophilus HB27. The TthPrimPol enzyme is extremely tolerant to alterations of the nucleotides of the template nucleic acid. Therefore, in one aspect the invention discloses methods for replicating, amplifying and sequencing of damaged polynucleotide templates.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 12, 2022
    Assignee: 4BASEBIO SL
    Inventors: Angel J. Picher, Luis Blanco
  • Patent number: 11293020
    Abstract: Provided herein, among other things, is an automatable procedure that employs in vitro directed evolution to create DNA sequences that encode a ligand-responsive ribozyme and which, when transcribed, can control expression of genes they are coupled to. The method also allows creation of functional RNA sequences that bind target molecules, without requiring any modification or immobilization of the target.
    Type: Grant
    Filed: May 27, 2020
    Date of Patent: April 5, 2022
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Brent Townshend, Christina D. Smolke
  • Patent number: 11273443
    Abstract: A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.
    Type: Grant
    Filed: October 14, 2020
    Date of Patent: March 15, 2022
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Keith E. Moravick, Brian Ciopyk, Victor Briones, Gregory Loney, Adam De La Zerda, Jesus Ching, Steven Chu, David Swenson, Helen Huang, Colin Kelly
  • Patent number: 11274327
    Abstract: Compositions, methods and kits are disclosed for synthesizing and amplifying pools of probes using precursor oligonucleotides. In some aspects the precursor is amplified and nicking enzymes are used to separate the full length probes from the amplification products. The methods enable the preparation of single stranded DNA probes of defined sequence and length that are suitable for use in target detection assays.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: March 15, 2022
    Inventors: Yuker Wang, Keith W. Jones, Ronald J. Sapolsky
  • Patent number: 11268139
    Abstract: Provided is a nucleic acid isothermal self-amplification method comprising, adding suitable palindrome complementary sequences at both ends of a target template to form a stem-loop structure spontaneously, and providing reagents and conditions as needed to perform self-amplification. The method does not require addition of additional amplification primers. The reagent comprises a DNA polymerase having a strand displacement activity. The method does not rely on exogenous amplification primers for amplification, has a constant amplification temperature without a complex temperature control equipment, and achieves rapid amplification. The amplification product is a long single-stranded DNA of a continuous complementary sequence and can be applied to special occasions. In addition, the amplification has no GC bias.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: March 8, 2022
    Inventor: Xinhua Lu
  • Patent number: 11264222
    Abstract: Systems and methods are described for heating sample transfer lines between a source of a sample and a detection system to detect analytes of interest in the sample, where the sample is maintained in a heated state to maintain dissolved analytes of interest in solution.
    Type: Grant
    Filed: August 14, 2020
    Date of Patent: March 1, 2022
    Assignee: Elemental Scientific, Inc.
    Inventors: Kyle W. Uhlmeyer, Tyler Yost, Jacob Unnerstall, Aaron Williams
  • Patent number: 11254978
    Abstract: Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (PCR). In one aspect, a method for cycling PCR includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first pH level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second pH level of the electrolytic fluid, in which the second pH level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: February 22, 2022
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Inanc Ortac, Sadik C. Esener
  • Patent number: 11242562
    Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.
    Type: Grant
    Filed: August 30, 2018
    Date of Patent: February 8, 2022
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Jesse Salk, Lawrence A. Loeb, Michael Schmitt
  • Patent number: 11236384
    Abstract: The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.
    Type: Grant
    Filed: January 16, 2018
    Date of Patent: February 1, 2022
    Assignee: ASURAGEN, INC.
    Inventors: Gary J. Latham, Richard Andrew Blidner, Liangjing Chen
  • Patent number: 11236383
    Abstract: Methods, compositions, and kits are provided for quantification of genome editing.
    Type: Grant
    Filed: March 13, 2019
    Date of Patent: February 1, 2022
    Assignees: Bio-Rad Laboratories, Inc., J. David Gladstone Institute
    Inventors: Jennifer Berman, Samantha Cooper, George Karlin-Neumann, Yuichiro Miyaoka, Bruce Conklin, Josh Shinoff
  • Patent number: 11230731
    Abstract: Compositions and methods, systems, and kits for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, e.g., due to gene duplication, or to variations from the normal euploid complement of chromosomes, e.g., trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: January 25, 2022
    Inventors: Matthew Sekedat, Jeffrey Buis, Ronald David Beaubien, Jr., Sharat Singh, Jeff Perry
  • Patent number: 11230730
    Abstract: A reagent solution includes water, a nucleotide, and tris(2-carboxyethyl)phosphine in a range of 0.5 ?M to 1000 ?M. The reagent solution can further include a non-ionic surfactant in an amount of 0.001% to 1% or a biocidal agent in an amount of 0.001% to 1%. The reagent solution can include salts, such as sodium chloride or magnesium sulfate.
    Type: Grant
    Filed: October 16, 2019
    Date of Patent: January 25, 2022
    Assignee: Life Technologies Corporation
    Inventors: Jamsheed Ghadiri, Karta Atehortua-Khalsa
  • Patent number: 11203786
    Abstract: The present invention provides detection systems and methods for detection of loci and genomic regions in a sample, including mixed samples, using hybridization to an array.
    Type: Grant
    Filed: January 13, 2020
    Date of Patent: December 21, 2021
    Assignee: Ariosa Diagnostics, Inc.
    Inventors: Arnold Oliphant, Jacob Zahn, Kara Jeneau, Patrick Bogard, Stephanie Huang
  • Patent number: 11193153
    Abstract: The present disclosure provides, in some embodiments, methods and compositions for exponential amplification of single- and double-stranded DNA under isothermal conditions.
    Type: Grant
    Filed: March 19, 2018
    Date of Patent: December 7, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Feng Xuan, Jocelyn Yoshiko Kishi, Peng Yin
  • Patent number: 11186836
    Abstract: The present disclosure relates to multifunctional molecules, including molecules according to formula (I): ([(B1)M-D-L1]Y-H1)O-G-(H2-[L2-E-(B2)K]W)P, ??(I) wherein G, H1, H2, D, E, B1, B2, M, K, L1, L2, O, P, Y, and W are defined herein. The present disclosure also relates to methods of preparing and using such multifunctional molecules to identify encoded molecules capable of binding target molecules.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: November 30, 2021
    Assignee: Haystack Sciences Corporation
    Inventor: Richard Edward Watts
  • Patent number: 11186862
    Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: November 30, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti, George Karlin-Neumann
  • Patent number: 11180796
    Abstract: Techniques regarding screening for mutations using nanoscale deterministic arrays are provided. For example, one or more embodiments described herein can comprise a method, which can comprise cleaving a deoxyribonucleic acid segment hybridized with a molecular probe to form a sample fluid. The cleaving can occur at a first end and a second end of the molecular probe. Also, the cleaving can comprise a cleaving agent that targets base pair mismatches. The method can also comprise supplying the sample fluid to a nanoscale deterministic lateral displacement array to screen for a single nucleotide polymorphism.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: November 23, 2021
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Stacey Gifford, Benjamin Wunsch, Joshua T. Smith, Sung-Cheol Kim
  • Patent number: 11180787
    Abstract: Methods for amplification of a target nucleic acid sequence comprising strand invasion are provided in which strand invasion occurs both at upstream and downstream regions of the target nucleic acid sequence. Further provided are kits and compositions suitable for use in such methods. The methods may comprise amplifying a target nucleic acid sequence comprising a region of unknown sequence, or determining the sequence of a target nucleic acid comprising a region of unknown sequence.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: November 23, 2021
    Assignee: AIDIAN OY
    Inventors: Kevin Eboigbodin, Mirko Brummer
  • Patent number: 11171962
    Abstract: Sharing data is disclosed. In some cases, sharing data includes receiving a request to share data from a first account to a second account, receiving an indication of a plurality of first account profiles associated with the first account to share with the second account, and establishing sharing from the plurality of first account profiles to the second account, wherein sharing comprises the second account having read access to a subset of nonpublic data associated with the plurality of first account profiles.
    Type: Grant
    Filed: April 28, 2021
    Date of Patent: November 9, 2021
    Assignee: 23andMe, Inc.
    Inventors: Brian Lee Hawthorne, Oleksiy Khomenko, Jeffrey Mellen, Marcela Miyazawa, Michael Polcari, Jack Tihon, Alexander Wong, Anne Wojcicki, Linda Avey
  • Patent number: 11162146
    Abstract: Provided herein are reagents for improving PCR accuracy.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: November 2, 2021
    Assignee: Brandeis University
    Inventors: John Rice, Lawrence Wangh, Arthur H. Reis, Jr., Kenneth Pierce, Cristina Hartshorn, J. Aquiles Sanchez, Stephen Van Hooser, Skye Fishbein
  • Patent number: 11162115
    Abstract: Systems, networks, methods, compositions and recombinant hosts for biosynthesizing hydrocarbons from a feedstock, such as gas, are provided.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: November 2, 2021
    Assignee: INV Nylon Chemicals Americas, LLC
    Inventors: Alexander Brett Foster, Leonard Keith Pattenden, Jonathan David Rand, Ana Teresa dos Santos Brito Mendes Roberts, Andrew Shaw, Mark Paul Taylor
  • Patent number: 11155792
    Abstract: The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black® RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is ˜200-fold over background, which is considerably better than other donor-quencher pairs (STNRs ˜10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.
    Type: Grant
    Filed: February 12, 2020
    Date of Patent: October 26, 2021
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: John Chaput, Andrew Larsen, Matthew Dunn
  • Patent number: 11155811
    Abstract: Deep mutational scanning is a foundational tool for addressing functional consequences of large numbers of mutants, yet a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Provided herein are nicking saturation mutagenesis, a single-day, single-pot saturation mutagenesis method using routinely prepped plasmid dsDNA as input substrate. Reproducibility and convenience of the method are demonstrated through validation by an external research laboratory.
    Type: Grant
    Filed: August 28, 2018
    Date of Patent: October 26, 2021
    Assignee: Board of Trustees of Michigan State University
    Inventors: Justin Klesmith, James Stapleton, Timothy Whitehead, Emily Wrenbeck
  • Patent number: 11142785
    Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
    Type: Grant
    Filed: February 11, 2020
    Date of Patent: October 12, 2021
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
  • Patent number: 11111532
    Abstract: The invention relates to a new method of characterising a target ribonucleic acid (RNA) involving forming a complementary polynucleotide. The method uses a transmembrane pore.
    Type: Grant
    Filed: October 17, 2014
    Date of Patent: September 7, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, Daniel John Turner, James White
  • Patent number: 11079388
    Abstract: An imaging agent comprises (a) at least one target recognition moiety; (b) at least one observable moiety non-transiently bound to the target recognition moiety, and (c) at least one docking moiety bound to the target recognition moiety, wherein the docking moiety is capable of transiently binding at least one observable moiety. In some embodiments, the at least one target recognition moiety is an antibody or antigen binding fragment thereof.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: August 3, 2021
    Assignee: Ultivue, Inc.
    Inventor: Xi Chen
  • Patent number: 11071963
    Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: July 27, 2021
    Assignee: Gen9, Inc.
    Inventors: Joseph Jacobson, Larry Li-Yang Chu
  • Patent number: 11060152
    Abstract: A method of diagnosing or predicting a bladder cancer, or a risk of a developing a bladder cancer in a subject is provided, which method includes the detection of specific mutations in the FGFR3 gene in a first biological sample; and the measure of the degree of methylation of target genes in a biological sample obtained from the subject in a second biological sample.
    Type: Grant
    Filed: October 29, 2018
    Date of Patent: July 13, 2021
    Assignee: ONCODIAG
    Inventor: Jean-Pierre Roperch
  • Patent number: 11060131
    Abstract: A method and kit for the capture and purification of specific nucleic acids from a sample with affinity capture probes on a solid support and for the replication of said nucleic acids with a strand displacing polymerase, whereby a second primer complementary to a sequence in each of the target nucleic acids distinct from that bound by capture probes is also bound to the nucleic acid targets, and extension of one of the primers on each target effects the separation of the copied nucleic acid strands from the solid support. Incorporation of universal nucleic acid sequences during their replication enables the simultaneous and highly specific amplification of multiple nucleic acid target sequences with minimal production of artifacts.
    Type: Grant
    Filed: March 29, 2016
    Date of Patent: July 13, 2021
    Assignee: ANGLE EUROPE LIMITED
    Inventors: David Frederick Englert, Kelly Kai Yin Seto
  • Patent number: 11027282
    Abstract: The present invention provides a system for receiving biological sequence information and activating the synthesis of a biological entity. The system has a receiving unit for receiving a signal encoding biological sequence information transmitted from a transmitting unit. The transmitting unit can be present at a remote location from the receiving unit. The system also has an assembly unit connected to the receiving unit, and the assembly unit assembles the biological entity according to the biological sequence information. Thus, according to the present invention biological sequence information can be digitally transmitted to a remote location and the information converted into a biological entity, for example a protein useful as a vaccine, immediately upon being received by the receiving unit and without further human intervention after preparing the system for receipt of the information.
    Type: Grant
    Filed: July 19, 2017
    Date of Patent: June 8, 2021
    Assignee: Codex DNA, Inc.
    Inventors: J. Craig Venter, Daniel Gibson, John E. Gill
  • Patent number: 11028451
    Abstract: Methods for the rapid detection of the presence or absence of Mycobacterium tuberculosis (MTB) and other members of the MTB-complex in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the esxJ gene, along with kits are provided that are designed for the detection of MTB.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: June 8, 2021
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Jenny A. Johnson
  • Patent number: 11008567
    Abstract: Transposomes and oligonucleotide replacement methods to make DNA libraries that have distinct 5? and 3? tags, and to make directional libraries that are enriched for a desired strand.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: May 18, 2021
    Assignee: Illumina, Inc.
    Inventors: Igor Goryshin, Bradley Baas, Ramesh Vaidyanathan, Mark Maffitt
  • Patent number: 10999285
    Abstract: Sharing data is disclosed. In some cases, sharing data includes receiving a request to share data from a first account to a second account, receiving an indication of a plurality of first account profiles associated with the first account to share with the second account, and establishing sharing from the plurality of first account profiles to the second account, wherein sharing comprises the second account having read access to a subset of nonpublic data associated with the plurality of first account profiles.
    Type: Grant
    Filed: October 23, 2020
    Date of Patent: May 4, 2021
    Assignee: 23andMe, Inc.
    Inventors: Brian Lee Hawthorne, Oleksiy Khomenko, Jeffrey Mellen, Marcela Miyazawa, Michael Polcari, Jack Tihon, Alexander Wong, Anne Wojcicki, Linda Avey
  • Patent number: 10988797
    Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.
    Type: Grant
    Filed: December 4, 2019
    Date of Patent: April 27, 2021
    Assignee: Illumina, Inc.
    Inventors: Raquel Maria Sanches-Kuiper, Vincent Peter Smith, Sean Humphray
  • Patent number: 10982247
    Abstract: This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: April 20, 2021
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Mark Aaron Behlke, Joseph A. Walder, Richard Owczarzy, Scott D. Rose, Joseph R. Dobosy, Susan M. Rupp
  • Patent number: 10975430
    Abstract: The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3? ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: April 13, 2021
    Assignee: Illumina Cambridge Limited
    Inventors: Vincent Peter Smith, Claire Bevis-Mott, Jonathan Mark Boutell
  • Patent number: 10954551
    Abstract: Methods, systems, and reagents for real-time single molecule sequencing of nucleic acids, particularly long DNA molecules, is described. Preferably, such methods and systems combine FRET (Förster Resonance Energy Transfer)-based proximity sensing of labeled nucleotides in or near a DNA polymerase's active site with FRET-based monitoring of the conformational changes in the polymerase that occur during nucleotide incorporation.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: March 23, 2021
    Assignee: Singular Genomics Systems, Inc.
    Inventor: Eli Glezer
  • Patent number: 10954558
    Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: March 23, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Arkadiusz Bibillo, Pranav Patel
  • Patent number: 10954553
    Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.
    Type: Grant
    Filed: July 12, 2016
    Date of Patent: March 23, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shoulian Dong, Chunmei Liu
  • Patent number: 10947585
    Abstract: Disclosed is a nucleic acid amplification method. The method comprises: performing a first nucleic acid amplification using a target nucleic acid contained in a sample as a template; and performing a second nucleic acid amplification using the amplification product produced in the first nucleic acid amplification step as a template. In the first nucleic acid amplification, a DNA polymerase that selectively amplifies a nucleic acid not comprising a uracil base is used to produce an amplification product that does not comprise a uracil base. In the second nucleic acid amplification, (i) dUTP and/or a primer comprising a uracil base, and (ii) a DNA polymerase capable of amplifying a nucleic acid comprising a uracil base are used to produce an amplification product that comprises a uracil base.
    Type: Grant
    Filed: February 21, 2018
    Date of Patent: March 16, 2021
    Assignee: SYSMEX CORPORATION
    Inventors: Michitaka Notoya, Shuji Yamashita, Mika Yoshimura, Yutaka Maeda