Nucleotide Patents (Class 435/89)
  • Patent number: 9603977
    Abstract: A method comprises preventing or inhibiting bacterial adhesion and/or bacterial biofilm development by treating a substrate with a composition of a soluble group II capsular polysaccharide obtained from a bacterial strain.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: March 28, 2017
    Assignees: INSTITUT PASTEUR, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Jean-Marc Ghigo, Jaione Valle, Sandra Da Re
  • Patent number: 9603979
    Abstract: A method comprises preventing or inhibiting bacterial adhesion and/or bacterial biofilm development by treating a substrate with a composition of a soluble group II capsular polysaccharide obtained from a bacterial strain.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: March 28, 2017
    Assignees: INSTITUT PASTEUR, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Jean-Marc Ghigo, Jaione Valle, Sandra Da Re
  • Patent number: 9562224
    Abstract: Production of products by engineered bacteria is increased by regulating cellular respiration. Cellular respiration is controlled by reducing electron transfer enzyme activity. Some examples of electron transfer enzymes include NADH dehydrogenases, Succinate dehydrogenases, ubiquinone synthesis, cytochrome O, and cytochrome D. In one example, deletion of UbiCA prevents respiration. Respiration can the be controlled by addition of ubiquinone or expression of ubiCA.
    Type: Grant
    Filed: September 7, 2007
    Date of Patent: February 7, 2017
    Assignee: William Marsh Rice University
    Inventors: Ka-Yiu San, George Bennett
  • Patent number: 9289003
    Abstract: A new type of cryoprotective agents that are useful for retaining the viability and metabolic activity of frozen or freeze-dried microbial cultures, is disclosed. The cryoprotective agent comprises compounds involved in biosynthesis of nucleic acids. Methods for the preparation as well as the uses of such cultures are given. Such cultures are useful as starter cultures in the manufacturing of food and feed products. Starter cultures of the invention include culture of lactic acid bacteria, e.g. Lactococcus species as well as other species.
    Type: Grant
    Filed: July 14, 2004
    Date of Patent: March 22, 2016
    Assignee: CHR. HANSEN A/S
    Inventors: Børge Windel Kringelum, Niels Martin Sørensen, Peter Sørensen
  • Publication number: 20150140561
    Abstract: The present invention provides deoxynucleoside tri- or tetraphosphate comprising a 3? nitrate and a detectable label covalently bound to the oxygen atom of an oxymethyl or oxyallyl or oxypropargyl substitution of a nucleobase. Such compounds provide new possibilities for future Sequencing by Synthesis technologies.
    Type: Application
    Filed: November 17, 2014
    Publication date: May 21, 2015
    Inventors: Frank Bergmann, Dieter Heindl, Jessica Steger-Domandl
  • Patent number: 9017980
    Abstract: Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: April 28, 2015
    Assignee: Crealta Pharmaceuticals LLC
    Inventors: Jacob Hartman, Simona Mendelovitz
  • Patent number: 9012182
    Abstract: Methods for producing purine nucleosides, and purine nucleotides, such as inosine and 5?-inosinic acid are provided which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine nucleoside productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. Also disclosed is the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.
    Type: Grant
    Filed: September 29, 2006
    Date of Patent: April 21, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Natalia Pavlovna Zakataeva, Vitaly Arkadievich Livshits, Sergey Viktorovich Gronsky, Ekaterina Aleksandrovna Kutukova, Anna Evgenievna Novikova, Yury Ivanovich Kozlov
  • Publication number: 20140356912
    Abstract: The present invention relates to the the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-?-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-?-D-glucopyranose. Methods for producing the sugars are also provided.
    Type: Application
    Filed: July 28, 2014
    Publication date: December 4, 2014
    Inventors: Christopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield
  • Patent number: 8852891
    Abstract: The present invention relates to a novel N-acetylglucosamine-2-epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N-acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: October 7, 2014
    Assignee: Gene Chem, Inc.
    Inventors: Jin Suk Woo, Jae Kyung Sohng, Byung Gee Kim, Sun Youp Kang, Dea Hee Kim, Kyoung Soon Jang, Ji Young Yang, Young Soo Jung, Won Min Seo, Tae Geon Gil, Sang Hee Shim, In Kang Hur
  • Patent number: 8846367
    Abstract: Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.
    Type: Grant
    Filed: November 17, 2011
    Date of Patent: September 30, 2014
    Inventors: Hideaki Yukawa, Masayuki Inui
  • Publication number: 20140287461
    Abstract: A method of producing a sugar liquid using a cellulose-containing biomass as a raw material includes (a) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution and (b) filtering the obtained aqueous sugar solution through a reverse osmosis membrane to collect a purified sugar liquid from a feed side, while removing fermentation-inhibiting substances from a permeate side.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 25, 2014
    Applicant: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Masateru Ito, Hideki Sawai, Masayuki Hanakawa, Shin-ichi Minegishi, Katsushige Yamada
  • Patent number: 8815545
    Abstract: Disclosed is a novel microorganism which has a malate dehydrogenase activity higher than that of a wild-type. Also, a recombinant vector which has the structure shown in the cleavage map of FIG. 1, a Corynebacteria strain transformed therewith, and a method of producing 5?-xanthosine monophosphate by culturing the transformed strain are disclosed.
    Type: Grant
    Filed: December 17, 2009
    Date of Patent: August 26, 2014
    Assignee: CJ CheilJedang Corporation
    Inventors: Jinman Cho, Hye Won Kim, Jinman Lee, Ji-Hye Lee, Yoon Seok Oh, Jang Hee Park
  • Publication number: 20140220638
    Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher Pmax per unit chlorophyll with little to no reduction in Pmax per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.
    Type: Application
    Filed: December 6, 2013
    Publication date: August 7, 2014
    Applicant: Synthetic Genomics, Inc.
    Inventors: Shaun Bailey, Jay McCarren, Soyan Leung Lieberman, Jonathan E. Meuser, Anna E. Romano, Daniel Yee, Leah Soriaga, Robert C. Brown, Joseph C. Weissman, Roger C. Prince, Robert D. Nielsen, Ariel S. Schwartz
  • Publication number: 20140193388
    Abstract: The invention relates to phosphatases and more in specific to (genetically) modified phosphatases, pharmaceutical compositions comprising (genetically) modified phosphatases and the use of (genetically) modified phosphatases for treating or curing for example sepsis, inflammatory bowel disease or other inflammatory diseases, or renal failure. The invention further relates to a method for producing phosphatases.
    Type: Application
    Filed: September 6, 2013
    Publication date: July 10, 2014
    Applicant: AM-PHARMA B.V.
    Inventors: MARKWIN PAUL VELDERS, LUIGI JOHANNES CORNELIUS JONK, WILLEM RAABEN, MARTY BERNARDUS FRANSISCUS WULFERINK
  • Publication number: 20140170711
    Abstract: A process for making a nucleotide analog includes combining a first substrate that includes a linker and a base with a second substrate to form a substrate composition. An enzyme contacts the substrate composition and catalyzes formation of the nucleotide analog from the first substrate and the second substrate. Additionally, a composition includes the first substrate, second substrate, the enzyme, the nucleotide analog, and optional additives.
    Type: Application
    Filed: February 7, 2014
    Publication date: June 19, 2014
    Applicant: NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY
    Inventors: JOHN P. MARINO, ZVI KELMAN, JERARD HURWITZ, GARY G. GIULIAN
  • Patent number: 8741599
    Abstract: A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproduc
    Type: Grant
    Filed: November 27, 2006
    Date of Patent: June 3, 2014
    Assignee: BASF SE
    Inventors: Markus Pompejus, Stephan Freyer, Markus Lohscheidt, Oskar Zelder, Matthias Boy
  • Patent number: 8703462
    Abstract: An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: April 22, 2014
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Chudi Guan
  • Patent number: 8685929
    Abstract: This invention pertains to the discovery that an amplification of the CYP24 gene or an increase in CYP24 activity is a marker for the presence of, progression of, or predisposition to, a cancer (e.g., breast cancer). Using this information, this invention provides methods of detecting a predisposition to cancer in an animal. The methods involve (i) providing a biological sample from an animal (e.g. a human patient); (ii) detecting the level of CYP24 within the biological sample; and (iii) comparing the level of CYP24 with a level of CYP24 in a control sample taken from a normal, cancer-free tissue where an increased level of CYP24 in the biological sample compared to the level of CYP24 in the control sample indicates the presence of said cancer in said animal.
    Type: Grant
    Filed: April 10, 2012
    Date of Patent: April 1, 2014
    Assignee: The Regents of the University of California
    Inventors: Donna G. Albertson, Daniel Pinkel, Colin Collins, Joe W. Gray, Bauke Ylstra
  • Patent number: 8546105
    Abstract: Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
    Type: Grant
    Filed: August 9, 2010
    Date of Patent: October 1, 2013
    Assignees: The University of Wyoming, The John Hopkins University, Human Genome Sciences, Inc.
    Inventors: Donald Jarvis, Michael J. Betenbaugh, Shawn Lawrence, Yuan C. Lee, Timothy A. Coleman
  • Patent number: 8541205
    Abstract: Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.
    Type: Grant
    Filed: May 1, 2012
    Date of Patent: September 24, 2013
    Assignee: Savient Pharmaceuticals, Inc.
    Inventors: Jacob Hartman, Simona Mendelovitz
  • Publication number: 20130245102
    Abstract: This disclosure describes unique single stranded DNA templates having a characteristic sequence and secondary structure. The DNA templates disclosed herein are useful for making small RNA molecules through promoterless transcription by a mammalian RNA polymerase, and can serve as an effective vector for producing small RNA molecules of interest in vitro, in situ and in vivo in mammalian cells.
    Type: Application
    Filed: October 11, 2011
    Publication date: September 19, 2013
    Applicant: Research Foundation of the City University of New York
    Inventors: Kevin Ryan, Christine I. Seidl
  • Patent number: 8530200
    Abstract: Disclosed is a method of producing 5?-guanosine monophosphate using a novel microorganism which has a malate dehydrogenase activity higher than that of a wild-type, thereby showing improved ATP productivity. Also, a novel microorganism is disclosed. The method comprises: culturing the corynebacteria strain which is enhanced in malate dehydrogenase activity over the endogenous activity, thus producing ATP in high yield; producing XMP in the culture; adding to the culture an enzyme or microorganism having XMP amination activity; and obtaining GMP from the culture.
    Type: Grant
    Filed: December 17, 2009
    Date of Patent: September 10, 2013
    Assignee: CJ Cheiljedang Corporation
    Inventors: Jinman Cho, Hye Won Kim, Yoon Seok Oh, Jang Hee Park
  • Patent number: 8501490
    Abstract: Polymeric sequence probes and methods are described that enhance the speed and sensitivity of detection of target analytes by combining a multiplicity of binding moieties specific for analyte, at least two of which are linearly arranged and optionally a multiplicity of detectable labels.
    Type: Grant
    Filed: April 19, 2010
    Date of Patent: August 6, 2013
    Assignee: The Regents of the University of California
    Inventors: Yong Chen, Suxian Huang
  • Patent number: 8440431
    Abstract: Materials and methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: May 14, 2013
    Assignees: Regents of the University of Minnesota, Iowa State University Research Foundation, Inc.
    Inventors: Daniel F. Voytas, Adam J. Bogdanove, Feng Zhang
  • Patent number: 8440432
    Abstract: Materials and methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: May 14, 2013
    Assignees: Regents of the University of Minnesota, Iowa State University Research Foundation, Inc.
    Inventors: Daniel F. Voytas, Adam J. Bogdanove, Feng Zhang
  • Patent number: 8435763
    Abstract: This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme sour
    Type: Grant
    Filed: September 14, 2004
    Date of Patent: May 7, 2013
    Assignee: Kyowa Hakko Bio Co., Ltd.
    Inventors: Satoshi Koizumi, Katsutoshi Sasaki, Tetsuo Endo, Kazuhiko Tabata, Akio Ozaki
  • Publication number: 20130078229
    Abstract: The present invention is directed toward the delivery of toxic agents to pathogenic cells, particularly cancer cells. In some embodiments, the toxic agent is a human ribonuclease or similar agent that is toxic to cells.
    Type: Application
    Filed: October 26, 2012
    Publication date: March 28, 2013
    Inventors: John A Kink, Laura E. Strong, Mark N. Shahan
  • Publication number: 20130065270
    Abstract: The present invention relates to methods for producing Saccharomyces strains that are capable of growth on xylose as a sole carbon source at a desired growth rate, (such as at least one generation per 48 hours), strains made by such methods, and Saccharomyces strains that grow at a growth rate of at least one generation per 48 hours using xylose as a sole carbon source for growth made by non-recombinant methods.
    Type: Application
    Filed: August 20, 2012
    Publication date: March 14, 2013
    Applicant: Microbiogen Pty Ltd.
    Inventors: Philip John Livingstone Bell, Paul Victor Attfield
  • Publication number: 20130059332
    Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 7, 2013
    Applicant: BASF SE
    Inventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jae Yeol Seo, Jörg Braun, Mo Se Kim, Sung Hyun Kim, Arno Kochner
  • Publication number: 20130012471
    Abstract: The invention provides for an in vitro method for producing capsular polysaccharides of Neisseria meningitidis. The invention also provides capsular polysaccharides obtainable by the methods described herein. The capsular polysaccharides comprise capsular polysaccharide specific for Neisseria meningitidis serogroups W-135, Y, X and A. Also encompassed are chimeric capsular polysaccharides comprising or composed of CPS of Neisseria meningitidis serogroups Y/W-135, W-135/Y, B/Y, C/Y, B/W-135, C/W-135, B/Y/W-135, C/Y/W-135, B/W-135/Y, C/W-135/Y. X/A or A/X. The invention also provides for the use of these capsular polysaccharides foi as pharmaceuticals, particularly as vaccines and/or diagnostics.
    Type: Application
    Filed: August 26, 2010
    Publication date: January 10, 2013
    Inventors: Rita Gerardy-Schahn, Martina Mühlanhoff, Andrea Bethe, Katharina Stummeyer, Friedrich Freiberger, Sebastian Damerow
  • Publication number: 20130004979
    Abstract: The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemo-enzymatic glycorandomization.
    Type: Application
    Filed: June 13, 2012
    Publication date: January 3, 2013
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: Jon S. Thorson, Richard W. Gantt, Pauline Marie Jeanne Peltier-Pain
  • Patent number: 8338185
    Abstract: Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: December 25, 2012
    Assignee: Isis Pharmaceuticals, Inc.
    Inventors: Walter F. Lima, Timothy Vickers, Stanley T. Crooke
  • Patent number: 8318436
    Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
    Type: Grant
    Filed: October 7, 2011
    Date of Patent: November 27, 2012
    Assignees: Sapphire Energy, Inc., The Scripps Research Institute
    Inventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
  • Patent number: 8303820
    Abstract: A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: November 6, 2012
    Assignee: Kyowa Hakko Bio Co., Ltd.
    Inventors: Hideki Murata, Tsuyoshi Mokudai, Michio Shiomi
  • Patent number: 8298791
    Abstract: A purine-derived substance is produced by culturing a Bacillus bacterium which has an ability to produce a purine-derived substance and has enhanced activity of an enzyme of the oxidative pentosephosphate pathway. The purine-derived substance is produced in the medium or the bacterial cells, and can be collected from the medium or the bacterial cells.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: October 30, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Kiyoshi Matsuno, Yukiko Mori, Takayuki Asahara
  • Patent number: 8293504
    Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.
    Type: Grant
    Filed: July 4, 2008
    Date of Patent: October 23, 2012
    Assignee: BASF SE
    Inventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jae Yeol Seo, Jörg Braun, Mo Se Kim, Sung Hyun Kim, Arno Kochner
  • Publication number: 20120263648
    Abstract: The instant invention provides polyvalent RNA nanoparticles comprising RNA motifs as building blocks that can form RNA nanotubes. The polyvalent RNA nanoparticles are suitable for therapeutic or diagnostic use in a number of diseases or disorders.
    Type: Application
    Filed: June 16, 2010
    Publication date: October 18, 2012
    Applicants: REGENTS OF THE UNIVERSITY OF CALIFORNIA, SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
    Inventors: Bruce A. Shapiro, Yaroslava G. Yingling, Eckart Bindewald, Wojciech Kasprzak, Luc Jaeger, Isil Severcan, Cody Geary, Kirill Afonin
  • Patent number: 8263332
    Abstract: A mismatched end DNA ligase is provided, which ligates two single strands to each other at a high efficiency, even if the other two single strands are not compatible. In one embodiment, the polypeptides of the ligase are Ku, Cernunnos, and XRCC4/Ligase4 (XL). This association can ligate DNA ends with a 3? overhang to a recessed 5? end, to a blunt end, or to a compatible end. In another embodiment, the proteins are Ku, Cernunnos, XRCC4/Ligase4 (XL) and DNA-PK.
    Type: Grant
    Filed: October 11, 2007
    Date of Patent: September 11, 2012
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Chun Tsai, Seonhi Kim, Gilbert Chu
  • Patent number: 8227208
    Abstract: A process for the enantioselective reduction of compounds having a steroid structure (ABCD) including one or several heteroatoms, one or several double bonds and/or aromatic components in the ring structure and having at least one oxo group at positions 3, 7, 11, 12 or 17 in the steroid ring system or in the a-position of any carbon moiety of the steroid structure: comprising providing the oxosteroid compound in the reaction at a concentration of ?50 g/L, a reduced cofactor NADH or NADPH, a hydroxysteroid dehydrogenase and a secondary alcohol or cycloalknaol to effect the enantioselective reduction.
    Type: Grant
    Filed: April 11, 2007
    Date of Patent: July 24, 2012
    Assignee: IEP GmbH
    Inventors: Antje Gupta, Anke Tschentscher, Maria Bobkova
  • Patent number: 8227213
    Abstract: The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with ?1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes under stringent conditions to the nucleotide sequence shown in SEQ ID NO: 1 or 3 or a nucleotide sequence complementary to at least one of them.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: July 24, 2012
    Assignee: National Institute of Advanced Science & Technology
    Inventors: Hisashi Narimatsu, Akira Togayachi, Niro Inaba, Toru Hiruma, Yasuko Ishizuka
  • Publication number: 20120142061
    Abstract: A heat labile alkaline phosphatase enzyme and methods of using the same and kits including the same are disclosed. Specifically, a nucleotide sequence of, peptide sequence of, methods of using, and kits comprising, a heat labile alkaline phosphatase isolated from Colwellia psychrerythraea are provided. Methods of over-expression and purification of the recombinant alkaline phosphatase and mutants thereof are also disclosed. Methods of over-expressing and purifying commercially useful quantities of active recombinant heat labile alkaline phosphatase fusion enzymes from C. psychrerythraea, wherein the fusion enzymes comprise one or more heterologous leader sequences are disclosed. The disclosed C. psychrerythraea heat labile alkaline phosphatase has properties similar to shrimp alkaline phosphatase and can be substituted for shrimp alkaline phosphatase in assays involving the same.
    Type: Application
    Filed: January 23, 2012
    Publication date: June 7, 2012
    Applicant: Affymetrix, Inc.
    Inventors: Jeannine Muller-Greven, Marc A. Post, Christopher J. Kubu
  • Patent number: 8178322
    Abstract: A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
    Type: Grant
    Filed: August 14, 2009
    Date of Patent: May 15, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masayuki Araki, Yusuke Takahashi, Akihiro Watanabe, Fumito Ohnishi, Takahiro Asano, Kazuya Kondo, Wataru Hibino, Shintaro Iwatani, Satoshi Okutani
  • Patent number: 8173602
    Abstract: This invention pertains to the discovery that an amplification of the CYP24 gene or an increase in CYP24 activity is a marker for the presence of, progression of, or predisposition to, a cancer (e.g., breast cancer). Using this information, this invention provides methods of detecting a predisposition to cancer in an animal. The methods involve (i) providing a biological sample from an animal (e.g. a human patient); (ii) detecting the level of CYP24 within the biological sample; and (iii) comparing the level of CYP24 with a level of CYP24 in a control sample taken from a normal, cancer-free tissue where an increased level of CYP24 in the biological sample compared to the level of CYP24 in the control sample indicates the presence of said cancer in said animal.
    Type: Grant
    Filed: December 8, 2009
    Date of Patent: May 8, 2012
    Assignee: The Regents of the University of California
    Inventors: Donna G. Albertson, Daniel Pinkel, Colin Collins, Joe W. Gray, Bauke Ylstra
  • Publication number: 20120083600
    Abstract: The invention provides methods of removing contaminants from a mixture of a desired product and contaminants by pH adjustments and molecular weight cut-offs. The contaminants include phosphate groups, magnesium sulfate, sodium pyruvate and tetrasodium pyrophosphate groups. The desired product includes nucleotide sugars, glycolipids, LnNT, sialyl lactose, and salts.
    Type: Application
    Filed: August 23, 2011
    Publication date: April 5, 2012
    Applicant: NOVO NORDISK A/S
    Inventors: Michael Felo, Shawn DeFrees
  • Publication number: 20120046355
    Abstract: The present invention describes compounds of Formula I or a pharmaceutically acceptable salts or derivatives thereof. Compositions comprising compounds of Formula I are also described. The present invention further relates to a method of producing non-2-enonate compounds.
    Type: Application
    Filed: May 4, 2010
    Publication date: February 23, 2012
    Inventors: Ian C. Schoenhofen, Dennis M. Whitfield, Susan M. Logan
  • Patent number: 8093061
    Abstract: Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.
    Type: Grant
    Filed: October 18, 2007
    Date of Patent: January 10, 2012
    Assignee: Isis Pharmaceuticals, Inc.
    Inventors: Walter F Lima, Timothy Vickers, Stanley T Crooke
  • Patent number: 8084068
    Abstract: The present invention describes a process to produce a composition containing 5?-ribonucleotides wherein a microorganism is subjected to autolysis under conditions at which a substantial part of the RNA remains in a form degradable into 5?-ribonucleotides and at which a substantial part of the RNA remains associated with the cell wall fraction. Said cell wall fraction is recovered by a solid/liquid separation method and the RNA associated with said wall fraction is converted into 5?-ribonucleotides. The present invention also describes compositions containing 5?-ribonucleotides and their use in food or feed.
    Type: Grant
    Filed: January 6, 2005
    Date of Patent: December 27, 2011
    Assignee: DSM IP Assets B.V.
    Inventors: Bertus Noordam, Jan Gerrit Kortes
  • Publication number: 20110306074
    Abstract: The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemoenzymatic glycorandomization.
    Type: Application
    Filed: June 13, 2011
    Publication date: December 15, 2011
    Applicant: Wisconsin Alumni Research Foundation
    Inventors: Jon S. Thorson, Richard W. Gantt
  • Publication number: 20110300582
    Abstract: Disclosed is a novel microorganism which has a malate dehydrogenase activity higher than that of a wild-type. Also, a recombinant vector which has the structure shown in the cleavage map of FIG. 1, a Corynebacteria strain transformed therewith, and a method of producing 5?-xanthosine monophosphate by culturing the transformed strain are disclosed.
    Type: Application
    Filed: December 17, 2009
    Publication date: December 8, 2011
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Jinman Cho, Hye Won Kim, Jinman Lee, Ji-Hye Lee, Yoon Seok Oh, Jang Hee Park
  • Publication number: 20110287488
    Abstract: The present invention relates to a novel N-acetylglucosamine-2-epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived from Bacteroides fragilis NCTC 9343, and a method for preparing CMP-N-acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.
    Type: Application
    Filed: December 7, 2007
    Publication date: November 24, 2011
    Applicant: GENE CHEM INC.
    Inventors: Jin Suk Woo, Jae Kyung Sohng, Byung Gee Kim, Sun Youp Kang, Dea Hee Kim, Kyoung Soon Jang, Ji Young Yang, Young Soo Jung, Won Min Seo, Tae Geon Gil, Sang Hee Shim, In Kang Hur