Carrier Is Synthetic Resin Patents (Class 436/531)
  • Patent number: 6248538
    Abstract: Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation.
    Type: Grant
    Filed: March 10, 1994
    Date of Patent: June 19, 2001
    Assignee: Mikrogen Molekularbiogische Entwicklungs - GmbH
    Inventors: Manfred Motz, Erwin Soutscheck, Renate Fuchs, Bettina Wilske, Vera Preac-Mursic
  • Patent number: 6242265
    Abstract: The use of calcium salts, magnesium salts or combination thereof in immunochemical methods for the determination of an analyte in a sample. These salts increase the specificity of such determinations, in particular, the background signal being reduced by addition of these salts in solid phase immunochemical methods.
    Type: Grant
    Filed: May 20, 1994
    Date of Patent: June 5, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventor: Bernhard Giesendorf
  • Patent number: 6242262
    Abstract: A method for isolating an active catalyst from a library of compounds that are potential catalysts is disclosed. The method involves providing a library which comprises a plurality of discrete solid supports, each solid support having a different organic compound bound thereto; and providing a reaction solution in a reaction vessel, the reaction solution containing the reactant or reactants necessary for a chemical reaction to occur in the presence of a catalyst for that reaction. The library and the reaction solution are then combined in the reaction vessel, and then one of the discrete solid supports is detected that is characterized by a temperature change in said solution greater than the temperature change of a plurality of other of said discrete solid supports in said solution. The detected solid support carries an active catalyst for the chemical reaction. Continuous flow apparatus for carrying out the method is also disclosed.
    Type: Grant
    Filed: October 24, 1997
    Date of Patent: June 5, 2001
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: James P. Morken, Steven J. Taylor
  • Patent number: 6242267
    Abstract: An apparatus and method for rapidly analyzing samples for analytes of interest by an homogeneous immunofluorescence assay. The apparatus includes a sample test cartridge having a high control sample section, a low control sample section, and at least one test sample section. Each of these sections contain at least one pre-loaded reagent housed in a well within the cartridge wherein the low control sample section contains a known low amount of an analyte of interest and the high control sample section contains a known high amount of an analyte of interest. The cartridge includes a biosensor comprising a planar waveguide having first and second parallel plane surfaces and an edge extending between them, the edge having a receiving region for receiving a light beam.
    Type: Grant
    Filed: September 18, 1998
    Date of Patent: June 5, 2001
    Assignee: University of Utah Research Foundation
    Inventors: James N. Herron, Douglas A. Christensen, Scott D. Miles
  • Patent number: 6238931
    Abstract: Particles and methods for the detection or visualization of analytes using fluorescence energy transfer. Particles comprising an energy donor as a first component and a fluorescent dye as a second component positioned in said particles at an energy exchanging distance from one another, wherein the two components have a Stokes shift of greater than or equal to 50 nm, said particle having bound on its surface, a protein, polypeptide, nucleic acid, nucleotide or protein containing ligand analogue are disclosed and claimed.
    Type: Grant
    Filed: July 12, 1994
    Date of Patent: May 29, 2001
    Assignee: Biosite Diagnostics, Inc.
    Inventors: Kenneth F. Buechler, Joseph Barry Noar, Lema Tadesse
  • Patent number: 6235464
    Abstract: A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.
    Type: Grant
    Filed: October 20, 1994
    Date of Patent: May 22, 2001
    Inventors: Glenn L. Henderson, Randal A. Hoke, Anne C. Hopkins, Daniel A. McLaurin
  • Patent number: 6235489
    Abstract: A method for determining whether a subject has had a stroke and, if so, the type of stroke which includes analyzing the subject's body fluid for at least four selected markers of stroke, namely, myelin basic protein, S100 protein, neuronal specific enolase and a brain endothelial membrane protein such as thrombomodulin or a similar molecule. The data obtained from the analyses provide information as to the type of stroke, the onset of occurrence and the extent of brain damage and allow a physician to determine quickly the type of treatment required by the subject.
    Type: Grant
    Filed: February 22, 2000
    Date of Patent: May 22, 2001
    Assignee: Syn X Pharma
    Inventor: George Jackowski
  • Patent number: 6232130
    Abstract: A method of evaluating a carbohydrate in a sample. The method includes providing a low valency carbohydrate binding ligand, providing a glycoconjugate which includes a label, and a carbohydrate moiety, contacting the low valency carbohydrate binding ligand and the glycoconjugate with the sample, determining the extent of binding of the low valency carbohydrate binding ligand with the glycoconjugate, the binding of the low valency carbohydrate binding ligand with the glycoconjugate being correlated with the amount of carbohydrate in the sample.
    Type: Grant
    Filed: June 4, 1998
    Date of Patent: May 15, 2001
    Assignee: Sensor Technologies, Inc.
    Inventor: David E. Wolf
  • Patent number: 6228660
    Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilized on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating mea
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: May 8, 2001
    Assignee: Conopco Inc.
    Inventors: Keith May, Michael Evans Prior, Ian Richards
  • Patent number: 6221612
    Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
    Type: Grant
    Filed: July 23, 1998
    Date of Patent: April 24, 2001
    Assignee: Aurora Biosciences Corporation
    Inventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Tim Rink
  • Patent number: 6214541
    Abstract: The present invention relates to a method of detecting specific antibodies directed against HPV proteins in body fluids, comprising the following steps: (I) incubating a native carrier material-bound HPV protein with body fluids, and (II) reacting specific antibodies (a) bound to the HPV protein with labeled antibodies (b) directed against antibodies (a) or with unlabeled antibodies (b) and the latter with labeled antibodies (c) directed against antibodies (b). Furthermore, this invention concerns a kit usable for this purpose.
    Type: Grant
    Filed: March 9, 1998
    Date of Patent: April 10, 2001
    Assignee: Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts
    Inventors: Hanswalter Zentgraf, Manfred Frey, Iris Velhagen, Regina Martens, Wolfgang Meschede, Michael Pawlita, Joris Braspenning, Masimo Tommasino
  • Patent number: 6214563
    Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents.
    Type: Grant
    Filed: July 21, 1998
    Date of Patent: April 10, 2001
    Assignee: Aurora Biosciences Corporation
    Inventors: Paul Negulescu, Gregor Zlokarnik, Tom Knapp, Roger Y. Tsien, Tim Rink
  • Patent number: 6210900
    Abstract: Invented is a method of preparing combinatorial libraries and combinatorial libraries prepared thereby. Also invented is a method for identifying compounds having desired characteristics from a combinatorial library or a set of combinatorial libraries by the use of flow cytometry. Also invented is a method for encoding combinatorial libraries using fluorophore labeled beads.
    Type: Grant
    Filed: November 22, 1996
    Date of Patent: April 3, 2001
    Assignee: SmithKline Beecham Corporation
    Inventors: Dennis Shinji Yamashita, Joseph Weinstock
  • Patent number: 6210978
    Abstract: Disclosed is a method for the immobilization of an analyte such as deoxypyridinium (DPD) onto a solid support. The method involves binding an antigen-amino acid-deoxypyridinium complex to the support via an anti-antigen binding partner located in a capture zone of the solid support. Preferably, the support is nitrocellulose and the antigen has the fluorescein structure.
    Type: Grant
    Filed: March 2, 1998
    Date of Patent: April 3, 2001
    Assignee: Bayer Corporation
    Inventors: Robert P. Hatch, Meitak Teresa Yip
  • Patent number: 6207398
    Abstract: The present invention provides novel cyclosporine C (CsC) derivatives having improved protein conjugatibility and hydrolytic stability. The present invention further provides a CsC derivative conjugated to a carrier, e.g., a solid support. Preferably, the solid support is a latex or magnetic particle.
    Type: Grant
    Filed: August 11, 1999
    Date of Patent: March 27, 2001
    Assignee: Dade Behring Inc.
    Inventor: Chengrong Wang
  • Patent number: 6207464
    Abstract: Boron complexes of certain bis-heterocyclic compounds are provided. The complexes resemble monomethine cyanines and are useful for imparting fluorescent properties to materials by covalent and noncovalent association. The compounds have the following general formula: wherein the dotted lines Z1 and Z2 represent the atoms necessary to complete a structure selected from the group consisting of one ring, two fused rings, and three fused rings, each said ring having five or six atoms, and each said ring comprising carbon atoms and, optionally, no more than two atoms selected from oxygen, nitrogen and sulfur, and R1 through R5 represent various atoms or groups and M is Cl or F.
    Type: Grant
    Filed: August 28, 1998
    Date of Patent: March 27, 2001
    Assignee: Carnegie Mellon University
    Inventors: Bhalchandra M. Karandikar, Alan S. Waggoner, Ratnakar B. Mujumdar
  • Patent number: 6204033
    Abstract: Spherically shaped polyvinyl alcohol (PVAL) polymer particles are prepared encapsulating a magnetic colloid that provides the particles with magnetic properties for use in binding biomolecules. The particles have a size of 1-10 &mgr;m that makes them particularly suitable for use in cell separation/sorting, cleaning biosubstances in suspension and diagnostic assays. The particles are prepared by dispersing a magnetic colloid containing a magnetic material such as a ferromagnetic or superparamagnetic substance in an aqueous solution of polyvinyl alcohol containing reactive hydroxyl groups, adding the resultant mixture to an organic phase containing a mixture of at least two emulsifiers, and adding a water-soluble cross-linking agent such as a dialdehyde that reacts with the hydroxyl groups of polyvinyl alcohol to form the polymer particles. The emulsifiers are miscible in the polyvinyl alcohol solution, and at least one emulsifier is semi-hydrophilic and at least one emulsifier is lipophilic.
    Type: Grant
    Filed: June 25, 1998
    Date of Patent: March 20, 2001
    Inventor: Detlef Müller-Schulte
  • Patent number: 6204367
    Abstract: An enzyme linked immunosorbent assay (ELISA) kit for the quantification of degradation products of carboxy-terminal telopeptides of type I collagen in a human serum sample, including an antibody that is characterized by binding to at least one peptide derived from the carboxy-terminal telopeptide domain of type I collagen and isolatable from a urine sample of a patient with active Paget's disease.
    Type: Grant
    Filed: February 10, 2000
    Date of Patent: March 20, 2001
    Assignee: Washington Research Foundation
    Inventor: David R. Eyre
  • Patent number: 6200762
    Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents.
    Type: Grant
    Filed: July 17, 1998
    Date of Patent: March 13, 2001
    Assignee: Aurora Biosciences Corporation
    Inventors: Gregor Zlokarnik, Paul Negulescu, Tom Knapp, Roger Y. Tsien, Tim Rink
  • Patent number: 6197494
    Abstract: An element and method for easily performing liquid assays are disclosed. The element uses capillary action to draw a predetermined volume of a liquid sample into a reaction chamber charged with reagent, where reaction between the liquid sample and the reagent is monitored.
    Type: Grant
    Filed: March 18, 1997
    Date of Patent: March 6, 2001
    Assignee: Cardiovascular Diagnostics, Inc.
    Inventor: Bruce Oberhardt
  • Patent number: 6197881
    Abstract: The invention pertains to an electrically conductive copolymer of the general formula I: wherein A is a first polymerizable monomer which produces an electrically conductive polymer when polymerized, and represents a polymerized unit of said monomer A in the electrically conductive polymer; B is a second polymerizable monomer which when copolymerized with monomer A produces an electrically conductive polymer, and represents a polymerized unit of said monomer B in the electrically conductive polymer; w is an integer greater than or equal to 0; x is an integer greater than or equal to 1; y is an integer greater than or equal to 0; z is an integer greater than or equal to I; 11, and 12 are each independently covalent linkers or spacer arms; 13 is substituent group having a desired chemical functionality; and Bt′ is selected from the group consisting of biotin and complexes of biotin with a molecule selected from the group consisting of avidin, streptavidin, derivatives of avidin
    Type: Grant
    Filed: August 18, 1999
    Date of Patent: March 6, 2001
    Assignee: Biopixel Ltd.
    Inventor: Serge Cosnier
  • Patent number: 6194552
    Abstract: The antitope of an antibody is masked with a masking agent, followed by immobilization on a support. The masking agent is then eluted to produce an improved immunosorbent, which is capable of binding more than double the amount of an antigen than existing immunosorbents having the same antibody bound at the same density. Preferably, the masking agent is an antigen or other compound having an epitope for which the antitope of the bound antibody has an avidity. In a preferred embodiment, greater than 30% of the bound antibodies maintain the same vicinity as when unbound for specific antigen or hapten molecules. Preferably, the support is formed of any conventional immunosorbent support material which allows the bound and unbound antibody to maintain an avidity for the same compounds or antigens.
    Type: Grant
    Filed: August 19, 1994
    Date of Patent: February 27, 2001
    Assignee: Center for Innovative Technology
    Inventors: William H. Velander, Carolyn Orthner
  • Patent number: 6194222
    Abstract: The invention relates in part to the use of independent assay controls (IACs) for the optical communication between an assay device and an instrument in monitoring and performing assays, preferably immunoassays.
    Type: Grant
    Filed: January 5, 1998
    Date of Patent: February 27, 2001
    Assignee: Biosite Diagnostics, Inc.
    Inventors: Kenneth F. Buechler, Joseph M Anderberg, Paul H. McPherson
  • Patent number: 6190923
    Abstract: The present invention relates to the field of immunoassays for metal ions. The invention presents: chelators, chelates, antibodies specific for the chelates, tracers comprising chelates conjugated to detectable labels, and immunoassays utilizing the foregoing.
    Type: Grant
    Filed: September 4, 1998
    Date of Patent: February 20, 2001
    Inventor: David K. Johnson
  • Patent number: 6190873
    Abstract: A method of measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is disclosed. A method of inactivating interfering cross-reactive material in an assay for measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is also disclosed. Compositions wherein cyclosporin is conjugated to an immunogenic carrier or a label, optionally through a linking group, at an alanine nitrogen atom of the cyclic backbone of cyclosporin are also disclosed. Compositions wherein atiocyclosporin is conjugated, optionally through a linking group, to an immunogenic carrier or a label are also disclosed. Where cyclosporin is conjugated to an immunogenic carrier, the conjugates may be used as immunogens for the preparation of antibodies which are capable of recognizing cyclosporin.
    Type: Grant
    Filed: March 10, 1995
    Date of Patent: February 20, 2001
    Assignee: Dade Behring Marburg GmbH
    Inventors: Dariush Davalian, Maureen H. Beresini, Svetlana Alexander, Mae Wan-Leng Hu, Edwin F. Ullman
  • Patent number: 6187598
    Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilised on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating mea
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: February 13, 2001
    Assignee: Conopco Inc.
    Inventors: Keith May, Michael Evans Prior, Ian Richards
  • Patent number: 6187599
    Abstract: Novel sensor devices composed of a crystalline colloidal array (CCA) polymerized in a hydrogel are disclosed. The hydrogels are characterized as being capable of shrinking and swelling in response to specific stimuli applied thereto. As the hydrogels shrink or swell, the lattice structure of the CCA embedded therein changes, thereby changing the wavelength of light diffracted by the CCA. Thus by monitoring the change in diffracted wavelength, the concentration of a stimulus is determined. The gels can be modified to sense numerous different stimuli. The sensor devices are specific in that they are modified to react with only one species or family of species. These sensors have various applications in areas including, for example, environmental and chemical systems, chemomechanical systems, sensor devices and medical diagnostic tools. Various methods for making and using these devices are also disclosed.
    Type: Grant
    Filed: July 7, 1998
    Date of Patent: February 13, 2001
    Assignee: University of Pittsburgh
    Inventors: Sanford A. Asher, John H. Holtz
  • Patent number: 6183772
    Abstract: The present invention provides compositions comprising colorimetric assay liposomes. The present invention also provides methods for producing colorimetric liposomes and calorimetric liposome assay systems. In preferred embodiments, these calorimetric liposome systems provide high levels of sensitivity through the use of dopant molecules. As these dopants allow the controlled destabilization of the liposome structure, upon exposure of the doped liposomes to analyte(s) of interest, the indicator color change is facilitated and more easily recognized.
    Type: Grant
    Filed: March 1, 1996
    Date of Patent: February 6, 2001
    Assignee: The Reagents of the University of California
    Inventors: Deborah Charych, Raymond C. Stevens
  • Patent number: 6180288
    Abstract: The present invention comprises an optically diffracting sensing device whose diffraction pattern changes upon exposure to some stimuli. The diffraction pattern may be two or three dimensional, and in one embodiment the change in diffraction patterns is recognizable to the untrained eye. The device comprises one or more gels coated onto patterned, self-assembling monolayers of alkanethiolates, carboxylic acids, hydroxamic acids, and phosphonic acids printed onto a variety of substrates, including glass, silicon, aluminum oxide, and thermoplastic films metallized with gold, or with an alloy such as nickel/gold. The present invention also comprises the method of making this device, and the use of this device.
    Type: Grant
    Filed: March 21, 1997
    Date of Patent: January 30, 2001
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: Dennis S. Everhart, Rosann M. Kaylor, Mark L. Jones
  • Patent number: 6180135
    Abstract: A direct assay is described using novel three-dimensional polymeric assemblies which change from a blue to red color when exposed to an analyte, in one case a flue virus. The assemblies are typically in the form of liposomes which can be maintained in a suspension, and show great intensity in their color changes. Their method of production is also described.
    Type: Grant
    Filed: October 6, 1997
    Date of Patent: January 30, 2001
    Assignee: The Regents of the University of California
    Inventors: Deborah Charych, Anke Reichert
  • Patent number: 6177241
    Abstract: This invention provides a method, a reagent, and a kit for detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk. The invention also provides a reagent for use in detecting herpesvirus-specific IgM antibodies indicative of recent infection while preventing detection of low levels of herpesvirus-specific IgM antibodies present in individuals of low risk.
    Type: Grant
    Filed: September 22, 1997
    Date of Patent: January 23, 2001
    Assignee: Abbott Laboratories
    Inventor: Gregory T. Maine
  • Patent number: 6174734
    Abstract: The invention features a novel method of assaying glycated hemoglobin (GHb) in a sample using a capture molecule that binds GHb and hemoglobin (Hb), and detecting bound GHb with a molecule that distinguishes GHb from non-glycated hemoglobin (Hb). The molecule used to distinguish GHb from Hb may be an antibody. The assay and antibodies of the invention are useful for the evaluation of GHb in disease states such as diabetes.
    Type: Grant
    Filed: October 24, 1997
    Date of Patent: January 16, 2001
    Assignee: Abbott Laboratories
    Inventors: Ralph K. Ito, Gordon Sanghera
  • Patent number: 6171782
    Abstract: A family of cDNA sequences derived from hepatitis C virus (HCV) are provided. These sequences encode antigens which react immunologically with antibodies present in individuals with non-A non-B hepatitis (NANBH), but which are absent from individuals infected with hepatitis A virus, or hepatitis B virus, and also are absent in control individuals. The HCV cDNA sequences lack substantial homology to the sequences of hepatitis delta virus (HDV) and HBV. A comparison of the sequences of amino acids encoded in the HCV cDNA with the sequences of Flaviviruses indicates that HCV may be related to the Flaviviruses. The HCV cDNA sequences and the polypeptides encoded therein are useful as reagents for the detection and therapy of HCV. The reagents provided in the invention are also useful for the isolation of NANBH agent(s), for the propagation of these agents in tissue culture, and for the screening of antiviral agents for HCV.
    Type: Grant
    Filed: May 15, 1995
    Date of Patent: January 9, 2001
    Assignee: Chiron Corporation
    Inventors: Michael Houghton, Qui-Lim Choo, George Kuo
  • Patent number: 6159748
    Abstract: Immunoassay methods and apparatus are provided which utilize flow cytometry, coated latex microspheres, and labelled antibodies, to simultaneously detect the presence and amount of several antigens or antibodies in a sample. Microspheres can be sized by forward angle light scatter (FALS) or electronic volume. By combining FALS and fluorescence, it is practical to use beads of several different sizes, colors or shapes, each bead coated with a different protein or antibody, for the simultaneous detection of multiple analytes in a sample. Available auto-sampling systems make it even more appealing in this regard. In accordance with one embodiment, highly purified RNP. Sm, SS-A, SS-B and Scl-70 antigens are bound to 4, 5, 6, 7 and 10 .mu.m latex beads, respectively and stabilized for extended shelflife. Diluted patient serum is placed into test tubes containing a mixture of the five antigen coated beads and incubated. If an antibody is present for a specific antigen, it will bind to that specific bead.
    Type: Grant
    Filed: June 1, 1998
    Date of Patent: December 12, 2000
    Assignee: AffiniTech, LTD
    Inventor: Mark K. Hechinger
  • Patent number: 6156550
    Abstract: A synthetic polymer based surface is coated with polymer particles without the use of an adhesive. A top surface layer is swollen or semidissolved by contacting with a solvent such as acetone, polymer particles are contacted with the surface to partially embed the particles in the surface layer, and the surface layer is dried. The polymer particles are either dry or in a slurry when contacted with the surface, and contact with the polymer particles may be simultaneous or subsequent to contacting with the solvent. The particles may be particles for chromatography, and may be derivatized before or after contacting with the surface such as by coupling an enzyme, DNA, an antibody or an antigen.
    Type: Grant
    Filed: January 26, 1998
    Date of Patent: December 5, 2000
    Assignee: Pharmacia Biotech AB
    Inventor: Gunnar Glad
  • Patent number: 6156270
    Abstract: The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.
    Type: Grant
    Filed: March 27, 1997
    Date of Patent: December 5, 2000
    Assignee: Biosite Diagnostics, Inc.
    Inventor: Kenneth Francis Buechler
  • Patent number: 6156524
    Abstract: Reagents and methods for the detection of Staphylococcus aureus are provided. The reagents contain an antibody that binds to a capsular polysaccharide of type 5 of Staphylococcus aureus, and can be used in methods for detection of oxacillin resistant Staphylococcus aureus that escapes detection by agglutination in the presence of fibrinogen and antibodies directed against protein A of Staphylococcus.
    Type: Grant
    Filed: May 30, 1995
    Date of Patent: December 5, 2000
    Assignee: Institut Pasteur
    Inventors: Jean-Michel Fournier, Alain Boutonnier
  • Patent number: 6153442
    Abstract: The present invention relates to compounds that are bis-biotins. These compounds comprise two biotinyl radicals connected by a chain of atoms, usually at least 16 atoms in length. The bis-biotin is conjugated to a member of a specific binding pair ("sbp member") wherein the chain is not part of the sbp member. Also disclosed are compositions comprising a complex of avidin and a bis-biotin as described above. The compounds and compositions of the invention find use in an assay for an analyte wherein there is employed a reagent system comprising an avidin reagent and a biotin reagent. The improvement of the present invention comprises using as the biotin reagent a bis-biotin as described above. Also disclosed are kits comprising the present bis-biotins and methods of preparing a bis-biotinylated conjugate of a member of a specific binding pair ("sbp member") for use in a specific binding assay.
    Type: Grant
    Filed: May 20, 1998
    Date of Patent: November 28, 2000
    Assignee: Dade Behring Inc.
    Inventors: Marcel Rene Pirio, Dariush Davalian, Jacqueline Sadakan Ishkanian, Edwin F. Ullman
  • Patent number: 6153113
    Abstract: A system and method are disclosed for separating particles having different sedimentation velocities. The system includes a container containing a binding substance including first particles and ligands attached to the first particles. When the binding substance is mixed with a liquid carrying at least second and third particles, the ligands bind the first and second particles together to form groups of bound particles. The groups of bound particles are separated from the third particles in a fluid chamber configured to be mounted on a centrifuge rotor. One of the disclosed methods includes forming a saturated fluidized bed of particles to retain the particles groups in the fluid chamber. Another of the disclosed methods includes removing at least some of the first particles after the binding substance is mixed with the liquid carrying second and third particles.
    Type: Grant
    Filed: February 22, 1999
    Date of Patent: November 28, 2000
    Assignee: Cobe Laboratories, Inc.
    Inventors: Raymond P. Goodrich, Todd Curtis Green
  • Patent number: 6150123
    Abstract: The present invention relates to a method for biotinylating a desired subset of a larger population of proteins, wherein the subset of proteins is first selectively bound to a solid phase, thereby separating it from undesired proteins of the population, and then, still bound to solid phase, it is biotinylated. Unreacted biotin may then be washed from the solid phase, and the biotinylated protein may be uncoupled from the solid phase.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: November 21, 2000
    Assignee: Centre Integre de Recherches Biocliniques sur le Sida (CIRBS)
    Inventor: Antonio Cosma
  • Patent number: 6143496
    Abstract: Methods of detecting and quantifying genomic nucleic acid molecule sequences are provided using the simultaneous amplification of a plurality of discrete nanoliter-sized samples. A miniaturized closed assembly is also provided for carrying out amplification of a nucleic acid molecule by polymerase chain reaction in multiple nanoliter-sized samples. Methods of filling miniaturized sample chambers are also provided as are methods for determining the number of template molecules in a sample by conducting replicate nucleic acid sequence amplification reactions on a set of terminally diluted samples and counting the number of positive amplification reactions. The methods can be used to detect a single starting nucleic acid target molecule.
    Type: Grant
    Filed: April 17, 1997
    Date of Patent: November 7, 2000
    Assignees: Cytonix Corporation, The United States of America as represented by the Department of Health and Human Services
    Inventors: James F. Brown, Jonathan E. Silver, Olga V. Kalinina
  • Patent number: 6143508
    Abstract: Devices, processes and compositions are provided for effective separation of cells from a mixture of cells, where depletion or positive selection may be employed to provide a cellular population of interest. Of particular utility is the separation of cells from peripheral blood mononuclear cells, where members of the lymphoid or myeloid lineages may be isolated and used for research, diagnosis or therapy. Also of interest are cellular separation from bone marrow, tumor suspensions or lymphoid tissue suspensions, where cells can be isolated and used for a variety of purposes. The separated cells may be homogeneous, free of exogenous biologicals, viable, capable of replication and exhibit their full complement of biological activities. Multiple phenotypes can be captured simultaneously. Captured cells can be specifically activated with cytokines and antigens to provide cells which are MHC restricted and have antigen-specific effector functions.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 7, 2000
    Assignee: Rhone-Poulenc Rorer Pharmaceuticals Inc.
    Inventor: Thomas B. Okarma
  • Patent number: 6143577
    Abstract: A process for magnetic immunoseparation of cells, in particular bacteria, fetal cells, stock cells of bone marrow and circulating cancerous cells, of the type consisting in affixing the target cells on paramagnetic balls and in causing a magnetic field to act on a sample containing the affixed cells, the free cells and the surplus paramagnetic balls, in order to isolate the paramagnetic balls, in that the sample is caused to circulate in a tube the section of which is much less than the length over which the magnetic field is applied.
    Type: Grant
    Filed: May 15, 1998
    Date of Patent: November 7, 2000
    Assignee: Biocom S.A.
    Inventor: Jean-Claude Bisconte Sconte De Saint Julien
  • Patent number: 6143506
    Abstract: The invention relates to methods and test kits for diagnosis of periodontal disease activity in mammals, especially in human. The methods of the invention provide for rapid chair-side diagnosis of periodontitis, peri-implantitis and HIV (+)-infection/AIDS-disease related periodontal diseases. Especially, the methods of the invention provide for rapid chair-side diagnosis of the loss of bone density associated with periodontal diseases.
    Type: Grant
    Filed: August 13, 1998
    Date of Patent: November 7, 2000
    Assignee: The Research Foundation of State of NY
    Inventors: Lorne M. Golub, Timo Sorsa, Olli Teronen, Sari Hannele Tikanoja
  • Patent number: 6140063
    Abstract: The present invention includes the method of treating a viral infection, specifically one occurring as a result of infection by a human immunodeficiency virus (HIV-1). The method of treatment depends upon the ligand binding of the Ah receptor. Transformation and translocation of the receptor and DNA binding are not required. The study of compounds that interact with the Ah receptor, either as agonists, or antagonists, has resulted in the identification of compounds with useful therapeutic properties through perturbation of viral pathogenic signal transduction pathways. Antagonists of the Ah receptor are more likely candidates for treatment because the toxicity of such compounds is low. Identification of molecules affecting cellular targets, such as the Ah receptor, that inhibit viral pathologic signaling would be of great therapeutic potential as the activity of these molecules is not directed against the virus itself, therefore genetic viral mutation to escape such therapy would be far less likely to occur.
    Type: Grant
    Filed: August 13, 1998
    Date of Patent: October 31, 2000
    Assignee: Paracelsian, Inc.
    Inventors: Geoffrey D. Wheelock, Joseph Rininger, John G. Babish, Padmasree Chigurupati
  • Patent number: 6140045
    Abstract: Materials and methods are provided for producing patterned multi-array, multi-specific surfaces which are electronically excited for use in electrochemiluminescence based tests. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use in flat panel displays and multiply specific testing procedures.
    Type: Grant
    Filed: March 6, 1997
    Date of Patent: October 31, 2000
    Assignee: Meso Scale Technologies
    Inventors: Jacob Wohlstadter, James Wilbur, George Sigal, Mark Martin, Liang-Hong Guo, Alan Fischer, Jon Leland
  • Patent number: 6136549
    Abstract: Novel magnetic assay methods and systems, as well as systems for conducting spectrophotometric analysis therewith. According to a preferred embodiment, the magnetic assay methods and systems incorporate a chromatographic medium, such as an assay test strip, that is designed to be contacted with a test solution having activated magnetic particles. A magnetic field, generated by a magnet or electromagnet, is additionally provided that if selectively applied to a chromatographic medium which causes the charged particles to become substantially bound at a site all in the chromatographic medium specified by the position of the magnets, to thus form a captured line or zone. To the degree of magnetic force applied to the medium may be selectively adjusted to vary the width or surface area of the capture line or zone. Additionally, in a preferred embodiment, capture lines may be formed while test strips are in motion along a stationary magnetized rail.
    Type: Grant
    Filed: October 15, 1999
    Date of Patent: October 24, 2000
    Inventor: Christopher C. Feistel
  • Patent number: 6136612
    Abstract: The present invention is directed to fluorescent dyes and its valence tautomers of the formula wherein Q represents a conjugated moiety that increases the fluorescent quantum yield of the compound; R.sub.1 is a functionalized group of the formula--(CH.sub.2).sub.j Y, wherein Y is selected from the group consisting of SO.sub.3 H, COOH, NH.sub.2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; R.sub.2 is a functionalized group of the formula --(CH.sub.2).sub.k Y', wherein Y' is selected from the group consisting of SO.sub.3 H, COOH, NH.sub.2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; M.sup.+ is a counterion selected from the group consisting of ammonium, alkali metal cations, and alkaline earth metal cations; n=1 to 4; m=1 to 4; j=2 to 10; and k=2 to 10.
    Type: Grant
    Filed: June 18, 1998
    Date of Patent: October 24, 2000
    Assignee: Sorin Biomedica Cardio S.p.A.
    Inventors: Leopoldo Della Ciana, Andrea Grignani, Mariacristina Cassullo, Giuseppe Caputo
  • Patent number: 6136551
    Abstract: An assay for the identification of neutralizing botulinum antibodies in sera is provided which includes the steps of separating non-sodium dodecyl sulfate, non-trypsinized complex botulinum toxin in an arylamide gel by electrophoresis, the separation occurring on a basis of botulinum toxin protein size and charge. Thereafter, the separated protein is electrophoretically transferred onto a solid support, the transferred separated protein being bound to the solid support at spaced apart sites. Remaining sites on the solid support not occupied by bound protein are blocked and the solid support and bound protein are contacted with a sera sample containing an antibody directed against botulinum toxin. The contacted solid support is then exposed to a second antibody capable of reacting to produce an insoluble colored substrate of intensity relative to a quantity of antibodies present. Finally, the second antibody is reacted to produce the insoluble colored substrate which is visually identified.
    Type: Grant
    Filed: March 17, 1998
    Date of Patent: October 24, 2000
    Assignee: ALLERGAN, Inc.
    Inventors: Kei Roger Aoki, Athena F. Spanoyannis
  • Patent number: 6136309
    Abstract: Antibodies directed against the ligand-binding component of the interferon (IFN)-.alpha./.beta. receptor (IFN.alpha./.beta.R, IFNAR2) are provided. The antibodies are capable of selectively modulating the activity of IFN-.alpha. as compared to IFN-.beta.. By virtue of their selectivity, the antibodies are useful in clinical and analytical applications.
    Type: Grant
    Filed: October 29, 1998
    Date of Patent: October 24, 2000
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Daniela Novick, Menachem Rubinstein