Extraction Processes (e.g., Solvent Extraction Process, Etc.) Patents (Class 536/25.41)
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Publication number: 20110281754Abstract: Disclosed are compositions and methods for isolating, detecting, amplifying, and quantitating Mycobacterium-specific nucleic acids in a sample. Also disclosed are compositions and diagnostic kits comprising Mycobacterium IS6110-specific oligonucleotide amplification primers and labeled oligonucleotide detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more tubercular pathogens, including Mycobacterium tuberculosis, in particular, from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.Type: ApplicationFiled: April 26, 2011Publication date: November 17, 2011Applicant: Longhorn Vaccines & Diagnostics, LLCInventors: Gerald W. Fischer, Luke T. Daum
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Publication number: 20110275090Abstract: A process for detecting the presence or absence of gram-positive bacteria in a biological sample. The biological sample can be obtained from any mammal and contains, at a minimum, cellular components. The sample is combined with an enzymatic lysing agent such as achromopeptidase, and lysed at room temperature. Ferric oxide is then added to the sample containing achromopeptidase. A magnetic field is applied to the sample and nucleic acids are extracted from the cellular components. Target nucleic acids, if present, are amplified using techniques such as Polymerase Chain Reaction (PCR) and then used to detect the presence or absence of gram-positive bacteria. Staphylococcus aureus and Streptococcus agalactiae are examples of target bacteria detected by the methods of the present invention.Type: ApplicationFiled: March 15, 2011Publication date: November 10, 2011Applicant: BECTON, DICKINSON AND COMPANYInventors: Danielle Hilligoss, Lisa M. Keller, Samah Ramadan, Jamie Coady, Tobin J. Hellyer
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Publication number: 20110275126Abstract: Provided are a simple method for producing a nucleic acid sample that does not need to use high-risk chaotropic salt in large amounts, does not need to use a limited special carrier, and offers a superior level of safety; and a method for producing a nucleic acid amplification product using the same. With respect to a cell sample containing cells, by releasing nucleic acid complexes from the cells and bringing this treatment liquid into contact with a carrier, the nucleic acid complexes are held in the carrier. Further, in the presence of a dispersion medium, by applying heat treatment to the carrier, DNAs such as genomic DNA and mitochondrial DNA, and RNA are released. Thereby, a nucleic acid sample can be recovered.Type: ApplicationFiled: January 15, 2010Publication date: November 10, 2011Applicant: ARKRAY, Inc.Inventor: Masahiro Kozuka
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Publication number: 20110266172Abstract: The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.Type: ApplicationFiled: July 14, 2011Publication date: November 3, 2011Inventors: Horst Donner, Frank Bergmann, Nina Lassonczyk, Manfred Watzele, Marcus Schmid
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Patent number: 8039613Abstract: A formulation containing guanidine thiocyanate together with acetamide, one or more acetamide derivatives, or a combination of acetamide and one or more acetamide derivatives is used to purify one or more nucleic acids contained in a medium. In particular, a medium containing at least one nucleic acid is combined with a binding matrix and the formulation in order to cause the at least one nucleic acid to separate from its in vivo cellular environment and to bind to the binding matrix. The binding matrix with at least one nucleic acid bound thereto then is separated from substantially the rest of the combined medium and formulation, after which the at least one nucleic acid is eluted from the binding matrix to obtain the at least one nucleic acid in a substantially purified form. If different nucleic acids are to be selectively purified from a single medium, multiple binding matrices, each compatible with a different nucleic acid, can be used.Type: GrantFiled: August 28, 2009Date of Patent: October 18, 2011Assignee: Promega CorporationInventor: Rex M. Bitner
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Publication number: 20110250680Abstract: The present invention relates to, among other things, a device for collecting airborne microorganisms, said device having: an air collecting module, comprising: i. an upper element having an air admission duct permitting entry of an air stream into said module, said duct being provided, at its base, with means for disturbance of the air stream, ii. a lower element having air evacuating means permitting the air stream created to exit and said upper and lower elements can be made integral with one another so that the air stream can be created within said air collecting module; a cartridge, of roughly cylindrical shape, having a microorganism retention zone, said retention zone having means for lysis of the microorganisms, said cartridge being positioned within said air collecting module.Type: ApplicationFiled: December 9, 2009Publication date: October 13, 2011Applicant: BIOMERIEUXInventors: Patrick Broyer, Agnes Dupontfilliard, Massimo Galdiero, Michel Guy, Hermanus Johannes Maria Kreuwel, Emiliano Maione, Emiel Gerebern Maria Verwimp
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Patent number: 8034924Abstract: Crystals of a purine nucleoside compound, particularly crystals of 2?,3?-dideoxyinosine, which have excellent storage stability and have a concentration of phosphate attached to the crystal of 25 ppm or more, may be produce by: (1) preparing an aqueous solution containing phosphate ion (PO43?) and a purine nucleoside compound; and (2) crystallizing the purine nucleoside compound from the aqueous solution.Type: GrantFiled: July 17, 2009Date of Patent: October 11, 2011Assignee: Ajinomoto Co., Inc.Inventors: Masaki Naito, Yoshitomo Kimura, Hiroya Ueda, Minoru Harada
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Publication number: 20110245483Abstract: The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.Type: ApplicationFiled: November 20, 2009Publication date: October 6, 2011Applicant: SIEMENS HEALTHCARE DIAGNOSTICS INC.Inventors: Heike Euting, Guido Hennig, Alexandre Izmailov
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Patent number: 8030479Abstract: Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.Type: GrantFiled: April 28, 2008Date of Patent: October 4, 2011Assignee: Samsung Electronics Co., Ltd.Inventors: Myo-yong Lee, Nam Huh, Joon-ho Kim
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Patent number: 8028591Abstract: Systems and methods are provided that can include a storage and retrieval robot operating under program control to extract a selective number of support beads from a storage or retainment region, for dispensing to titer wells or other vessels for assays and other purposes. According to various embodiments, the storage and retrieval robot can position a capture device over any one or more storage wells containing oligonucleotide or other material support beads, and withdraw or extract those support beads into a collection tube under vacuum pressure or other force. According to various embodiments, a selected number of support beads can be extracted, using a linear motor piston to limit available space for support bead insertion. According to various embodiments, the collected support beads can be dispensed into one or more destination tubes, wells, or other containers, surfaces, vessels, receptacles or mixture containment region, for use in assays or other purposes.Type: GrantFiled: May 11, 2007Date of Patent: October 4, 2011Assignee: Applied Biosystems LLCInventor: Charles S. Vann
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Publication number: 20110236895Abstract: The present invention relates to the providing of a method for preparing a sample from a nucleic acid-containing sample such as biological samples, where inhibitory substance's action against a enzyme reaction using a nucleic acid as substrate are decreased, a solution for preparing a sample used for the method, a stool collection kit used in that method, and a method for recovering and analyzing a nucleic acid in a nucleic acid-containing sample using a sample prepared using the preparation method of the present invention. A method for preparing a sample according to the present invention is a method for preparing a sample being used for analyzing a nucleic acid, and is characterized in that a nucleic acid-containing sample is mixed with a solution having one or more members selected from the group consisting of a polycation and a chelating agent as an active ingredient.Type: ApplicationFiled: June 3, 2011Publication date: September 29, 2011Applicant: OLYMPUS CORPORATIONInventors: Yasuo Tanigami, Tomonori Nagaoka
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Publication number: 20110229881Abstract: An ink, which contains DNA and is hardly decomposed by an external stimulus such as ultraviolet light, heat, an acid or an alkali, is provided. A method of easily analyzing a DNA in an ink composition is also provided. An ink composition containing a DNA and having a water-tolerance at a certain level or higher is prepared. The DNA in a print that is produced by using the above ink composition is quickly extracted with water or an aqueous solution and analyzed. Furthermore, a DNA in a print that is produced by using an oil- and water-based ink composition containing the DNA is quickly extracted with water or an aqueous solution and analyzed.Type: ApplicationFiled: September 11, 2008Publication date: September 22, 2011Applicants: NAGAHAMA BIO-LABORATORY INCORPORATED, EDUCATIONAL CORPORATION KANSAI BUNRI SOUGOUGAKUENInventors: Atsushi Oshima, Fumie Yusa
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Publication number: 20110224419Abstract: The present invention relates to a method for the isolation and purification of nucleic acids by elution of nucleic acids from nucleic acid-containing samples, and biological materials. The present invention further relates to a kit for carrying out the method of the invention.Type: ApplicationFiled: September 2, 2009Publication date: September 15, 2011Applicant: QIAGEN GMBHInventors: Ralf Himmelreich, Sabine Werner
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Publication number: 20110224420Abstract: The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with my colic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier.Type: ApplicationFiled: May 18, 2011Publication date: September 15, 2011Applicant: KONICA MINOLTA MEDICAL & GRAPHIC, INC.Inventors: Noriaki YAMAMOTO, Keigo TAMAKI, Koji MIYAZAKI, Akihisa NAKAJIMA
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Publication number: 20110203688Abstract: Processes for extracting nucleic acid from a biological sample and related assemblies and kits are disclosed. The processes comprise the steps of (a) providing a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface is composed of unmodified, smooth glass and defines a tubular lumen providing fluid communication between the first port and second port, wherein the lumen is circular, oval, or elliptical in cross-section, and wherein the lumen is essentially free of nucleic acid-specific binding sites; (b) introducing a nucleic acid-containing sample into the lumen of the device via the first port; (c) allowing nucleic acid in the sample to bind to the unmodified smooth glass surface; and (d) washing the bound nucleic acid.Type: ApplicationFiled: November 17, 2010Publication date: August 25, 2011Applicant: BLOOD CELL STORAGE, INC.Inventors: Michael W. Reed, Oliver Z. Nanassy, Paul V. Haydock, Daniel P. Gestwick
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Publication number: 20110200991Abstract: A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases.Type: ApplicationFiled: April 26, 2011Publication date: August 18, 2011Inventors: Timothy R. Hansen, Matthew P. Collis, Bradley S. Thomas, Thomas L. Fort
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Publication number: 20110196146Abstract: Methods and materials for improving nucleic acid or protein recovery from samples preserved in liquid cytological preservative solutions by utilizing scavenging agents, such as hydrazine- and hydrazide-containing compounds, are provided. Lysis solutions comprising hydrazine- and hydrazide-containing compounds and kits comprising the same are also provided.Type: ApplicationFiled: January 4, 2011Publication date: August 11, 2011Applicant: QIAGEN GAITHERSBURG INC.Inventors: Yuri KHRIPIN, Arvind Virmani, Lori Kobayashi
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Publication number: 20110195426Abstract: The present invention is partly based on the discovery that adverse factors can prevent an effective extraction of nucleic acids from a biological sample and that novel and unexpected agents and steps may be used to mitigate or remove the adverse factors, thereby dramatically improving the quality of the extracted nucleic acids. As such, one aspect of this invention is a novel method for extracting high quality nucleic acids from a biological sample. The high quality extractions obtained by the novel methods described herein are characterized by high yield and high integrity, making the extracted nucleic acids useful for various applications in which high quality nucleic acid extractions are preferred, e.g., a diagnosis, prognosis or therapy evaluation for a medical condition.Type: ApplicationFiled: July 16, 2010Publication date: August 11, 2011Applicant: THE GENERAL HOSPITAL CORPORATIONInventors: Leileata M. Russo, Kevin C. Miranda, Johan Skog
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Patent number: 7989614Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.Type: GrantFiled: March 24, 2008Date of Patent: August 2, 2011Assignee: Invitrogen Dynal ASInventors: Arne Deggerdal, Frank Larsen
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Publication number: 20110184162Abstract: The present invention relates to a method for rapid isolation of RNA. More particularly, it relates to a method for isolation of RNA using two-solution system. The present invention also relates to a RNA isolation kit.Type: ApplicationFiled: July 31, 2006Publication date: July 28, 2011Applicant: Council of Scientific and Industrial ResearchInventors: Sanjay Ghawana, Kashmir Singh, Jyoti Raizada, Arti Rani, Kumar Pradeep Bhardwaj, Sanjay Kumar
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Publication number: 20110165562Abstract: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.Type: ApplicationFiled: March 7, 2011Publication date: July 7, 2011Inventors: Farzad Pourahmadi, William A. McMillan, Jesus Ching, Ronald Chang, Lee A. Christel, Gregory T.A. Kovacs, M. Allen Northrup, Kurt E. Petersen
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Publication number: 20110165565Abstract: The present invention features methods and compositions for methylation detection, as well as a novel method for polynucleotide extraction and sodium bisulfite treatment.Type: ApplicationFiled: January 5, 2009Publication date: July 7, 2011Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: Tza-Huei Wang, Stephen Baylin, James Gordon Herman, Vasudev Bailey, Hariharan Easwaran, Hetty Carraway, Yi Zhang, Brian P. Keeley
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Publication number: 20110144286Abstract: Nucleic acid material can be effectively separated from a fluid by first contacting the fluid with a positively charged polymer which binds the nucleic acid material. Thereafter, the polymer, having the nucleic acid material bonded thereto, is contacted with a releasing agent which comprises a solution of an alkaline material and a glycol. The solution has a pH of no more than 12 and operates to release the nucleic acid material from the polymer under relatively low temperature conditions, typically no more than 50° C., and in particular instances, no more than 40° C. The glycol material may comprise a monomeric glycol such as ethylene glycol, propylene glycol, or the like, or it may comprise a polymeric glycol such as polyethylene glycol. Also disclosed is a novel positively charged polymer which may be employed in the separation process. This polymer comprises an acidified polyamine, such as polyethyleneimine which has been reacted with a nonacidified polyethyleneimine in a coupling reaction.Type: ApplicationFiled: December 13, 2010Publication date: June 16, 2011Inventor: Betty Wu
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Publication number: 20110097782Abstract: A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids.Type: ApplicationFiled: October 15, 2010Publication date: April 28, 2011Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Pei-Shin JIANG, Kun-Chan Wu, Yu-Ting Su, Chia-Yun Lin, Siou-Cing Su, Yuh-Jiuan Lin
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Publication number: 20110087016Abstract: An embodiment of a method for extracting biological material from an emulsion is described that comprises the steps of a) breaking an emulsion comprising a plurality of aqueous droplets in a continuous phase of an oil using a solvent to produce a combined aqueous-oil mixture, where the solvent disrupts the aqueous droplets which release a plurality of biological elements each immobilized on a substrate into the combined aqueous-oil mixture; b) introducing an inorganic salt to the combined aqueous-oil mixture causing a phase separation of the mixture into a first phase comprising an aqueous solution and the biological elements and a second phase comprising the solvent and the oil; c) extracting the first phase from the second phase; and d) collecting the substrate immobilized biological elements from the first phase.Type: ApplicationFiled: September 10, 2010Publication date: April 14, 2011Applicant: 454 LIFE SCIENCES CORPORATIONInventor: Yue Suo
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Patent number: 7923551Abstract: The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.Type: GrantFiled: April 28, 2008Date of Patent: April 12, 2011Assignee: Samsung Electronics Co., Ltd.Inventors: Myo-yong Lee, Nam Huh, Joon-ho Kim
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Publication number: 20110046361Abstract: The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids. The method includes first mixing a sample containing the double-stranded nucleic acid and the single-stranded nucleic acid with a pH-neutral, buffered solution consisting essentially of a chaotropic salt and a pH buffer to generate a mixture; then applying the mixture to a first mineral support for the double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes adjusting the pH of the flow-through to an acidic pH, and applying the acidified flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids bound can be eluted from the mineral supports respectively.Type: ApplicationFiled: April 22, 2009Publication date: February 24, 2011Applicant: GE Healthcare Bio-Sciences Corp.Inventor: Miao Jiang
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Patent number: 7884201Abstract: A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pellet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.Type: GrantFiled: August 30, 2006Date of Patent: February 8, 2011Assignee: Fujifilm CorporationInventors: Hiroko Inomata, Tomoko Mori
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Publication number: 20110015381Abstract: An instrument and a method for conveniently collecting nucleic acids from a biological nucleic acid-containing sample are provided. A nucleic acid-capturing tip having silica-containing solid phases enclosed therein in such a state as being capable of coming into contact with a liquid, wherein the solid phases have a water-flowing regions and the average interval among solid phases in the water-flowing regions is regulated to 25 ?m or less.Type: ApplicationFiled: August 26, 2010Publication date: January 20, 2011Inventors: Toshinari Sakurai, Toshiaki Yokobayashi
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Patent number: 7871764Abstract: The present invention relates to a method for extracting nucleic acids from biological samples. More specifically the invention relates to a universal method for extracting nucleic acids from unidentified biological samples. An advantage of the presently invented method is its ability to effectively and efficiently extract nucleic acids from a variety of different cell types including but not limited to prokaryotic or eukaryotic cells and/or recalcitrant organisms (i.e. spores). Unlike prior art methods which are focused on extracting nucleic acids from vegetative cell or spores, the present invention effectively extracts nucleic acids from spores, multiple cell types or mixtures thereof using a single method. Important that the invented method has demonstrated an ability to extract nucleic acids from spores and vegetative bacterial cells with similar levels effectiveness.Type: GrantFiled: March 18, 2008Date of Patent: January 18, 2011Assignee: The United States of America as represented by the United States Department of EnergyInventor: Sergei Bavykin
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Publication number: 20110009608Abstract: The present invention relates to an automatic refining apparatus for separating target materials from a plurality of biological sample solutions by using magnetic particles to which the magnetic particles are to be reversibly coupled, and to a multi-well plate kit for use in the automatic refining apparatus. Further, the present invention relates to a method for extracting nucleic acids from biological samples by using the above-described automatic refining apparatus. The present invention can be used in the automatic separation of nucleic acid, protein, and the like from biological samples.Type: ApplicationFiled: April 8, 2009Publication date: January 13, 2011Applicant: BIONEER CORPORATIONInventors: Jong-Hoon Kim, Jong Kab Kim, Yang Won Lee, Han Oh Park
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Publication number: 20100330701Abstract: The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70° C. or more.Type: ApplicationFiled: December 28, 2007Publication date: December 30, 2010Applicant: Japan Tobacco Inc.Inventors: Yoshimitsu Takakura, Satoru Usami, Masako Ichikawa
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Publication number: 20100310683Abstract: Pharmaceutical compositions or functional foods for treating depression and anxiety comprising ginseng saponin (Rg1+Rb1), glycyrrhizic acid and jujuba cAMP. Experiments demonstrate that as compared with the preferred drug for treating depression and anxiety paroxetine and diazepam in the art, the present invention has significant anti-depression and anxielytic efficacy.Type: ApplicationFiled: November 30, 2007Publication date: December 9, 2010Inventor: Zuoguang Zhang
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Publication number: 20100310682Abstract: Pharmaceutical compositions or functional foods for treating anxiety comprising ginseng saponin (Rg1+Rb1), glycyrrhizic acid and jujuba cAMP. Experiments demonstrate that as compared with the preferred drug for treating diazepam in the art, the present invention has significant anxielytic efficacy.Type: ApplicationFiled: November 30, 2007Publication date: December 9, 2010Inventor: Zuoguang Zhang
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Publication number: 20100311041Abstract: Dermatophytes which belong to one of the three genera Epidermophyton, Trichophyton and Microsporum are the main cause of fungal infections of skin, hair and nails. Traditional diagnostic procedures consist of microscopy and culture, but due to the slow growth rate of dermatophytes typically two to four weeks are needed before a final diagnosis is obtained. The present invention is a rapid DNA extraction method extracting nucleic acids from fungi (e.g. dermatophytes and other pathogenic fungi) which can be performed from directly on hair, nail or skin specimens from humans, from naturally or experimentally infected animals or from cultured fungal colonies for the use in PCR amplification and detection assays. The present invention also includes specific primer sets for detection of any dermatophyte and for species specific detection of Trichophyton rubrum and Epidermophyton floccosum by PCR and a kit for diagnosing fungal infections.Type: ApplicationFiled: June 13, 2006Publication date: December 9, 2010Applicant: STATENS SERUM INSTITUTInventor: Anna H. Brillowska-Dabrowska
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Publication number: 20100311039Abstract: Provided are methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, and methods and means for predicting the progression of precancerous cervical lesions.Type: ApplicationFiled: April 30, 2010Publication date: December 9, 2010Applicant: QIAGEN GAITHERSBURG INC.Inventors: Brian Lowe, Anna K. Fulbright, Irina Nazarenko
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Publication number: 20100305312Abstract: The invention relates to a method for the extraction and detection of nucleic acids on a membrane, making it possible to identify the microorganisms present in low concentration in a liquid or gaseous medium, as well as a novel lysis composition comprising guanidium chloride and between 0.1 and 1% N-Lauroyl-Sarcosine (NLS) making it possible to implement this method.Type: ApplicationFiled: August 3, 2010Publication date: December 2, 2010Applicant: MILLIPORE CORPORATIONInventors: Roxana Isac, Frederic Marc
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Publication number: 20100297708Abstract: The present invention relates to automated devices and methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. The invention is particularly suited for use in point-of-care medical diagnostics testing.Type: ApplicationFiled: July 29, 2010Publication date: November 25, 2010Applicant: ABBOTT POINT OF CARE INC.Inventors: Gordon Bruce Collier, John Allister Wood, Jason Andrew MacLeod, William Charles Dicke, Attila Csaba Nemeth, Cary James Miller
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Publication number: 20100297710Abstract: Provided herein are methods, compositions and kits to extract and relatively enrich by physical separation or amplification short base pair nucleic acid in the presence of a high background of genomic material (e.g., host or maternal nucleic acids).Type: ApplicationFiled: May 30, 2007Publication date: November 25, 2010Applicant: SEQUENOM, INC.Inventors: Carolyn R. Hoyal-Wrightson, Andreas Braun, Karsten E. Schmidt
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Publication number: 20100297733Abstract: Systems and methods are provided for capturing and/or isolating target microparticles. In one aspect, a method for capturing target microparticles is disclosed. The method includes: forming a fluid including the target microparticles, non-target microparticles, and magnetic beads, the magnetic beads having a stronger affinity with the target microparticles than with the non-target microparticles; flowing the fluid through a multidirectional microchannel; and applying a magnetic field to the fluid while the fluid is flowing through at least a portion of the microchannel to effect capture of at least a portion of the target microparticles onto the magnetic beads. Such a method can further includes passing the fluid having exited from the microchannel through a separator while subjecting the fluid to a second magnetic field so as to isolate the target microparticles. In addition, devices and systems are disclosed for capturing and/or isolating target microparticles based on magnetic manipulation.Type: ApplicationFiled: April 21, 2010Publication date: November 25, 2010Inventors: Qiao Lin, Yao Zhou
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Publication number: 20100298549Abstract: An extraction method for a target bio-molecule of a sample solution is described. First, a substrate having a thermally responsive polymer brush immobilized thereon is provided. The thermally responsive polymer brush has a lower critical solution temperature (LCST). Thereafter, while the sample solution flows over the substrate, the temperature of the sample solution or the substrate or both is decreased to less than the LCST, so that the target bio-molecule of the sample solution is captured inside the thermally responsive polymer brush. Afterwards, while the extraction solution flows over the substrate, the temperature of the extraction solution or the substrate or both is increased to more than the LCST, so that the captured target bio-molecule of the sample solution is released out of the thermally responsive polymer brush.Type: ApplicationFiled: May 20, 2009Publication date: November 25, 2010Applicant: National Taiwan University of Science and TechnologyInventor: Chien-Kuang Chen
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Patent number: 7833796Abstract: The invention concerns materials and methods used for the concentration, desalination, purification or stabilization of biological compounds, in particular biological macromolecules and supramolecular structures, by means of superabsorbent polymers or superabsorptive composite materials.Type: GrantFiled: December 15, 2004Date of Patent: November 16, 2010Assignee: Preentec AGInventors: Rene Pellaux, Jens-Martin Heile, Andreas Josef Schenzle, Martin Held
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Publication number: 20100285596Abstract: The invention provides methods of isolating, purifying, analyzing and/or detecting, functionalized macromolecules, e.g., peptides, phosphopeptides, polypeptides, proteins, oligonucleotides, or phospholipids in a sample, e.g., a biological mixture, using solid phase extraction with an alumina sorbent packed in a micro-elution plate.Type: ApplicationFiled: September 5, 2008Publication date: November 11, 2010Applicant: WATERS TECHNOLOGIES CORPORATIONInventors: Ying Qing Yu, Martin Gilar
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Patent number: 7829346Abstract: Disclosed herein is a method for extracting a biosubstance from a root of a hair, including the step of using as the hair a hair that has pulling force of at least a predetermined reference value to pull out the hair.Type: GrantFiled: February 19, 2008Date of Patent: November 9, 2010Assignee: Sony CorporationInventors: Takuro Yamamoto, Tomoteru Abe, Kazuhiro Nakagawa, Shiko Yamashita, Haruhiko Soma, Noriyuki Kishii
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Publication number: 20100280233Abstract: A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range.Type: ApplicationFiled: May 4, 2010Publication date: November 4, 2010Inventors: Dennis M. Connolly, Charles DeBoer, Vera Tannous
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Publication number: 20100267109Abstract: Compositions and methods for separating double-stranded nucleic acids out of a mixture comprising single-stranded nucleic acids and/or dNTPs and/or enzymes. The method uses spatially inhomogenously functionalized nanoporous materials. For example, the compositions and methods of the present invention can be used to purify DNA amplification reaction products.Type: ApplicationFiled: February 11, 2010Publication date: October 21, 2010Inventors: LEWIS ROTHBERG, BARBARA STWERTKA
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Patent number: 7815803Abstract: The present invention provides a novel procedure of preparing samples for analysis by way of mass spectrometry, preferably LC-MS/MS. Accordingly, functionalized magnetic particles with a hydrophobic surface were used for extracting low molecular weight compounds from complex liquid biological samples such as plasma, serum, whole blood or hemolyzed blood. The method of the invention includes (a) contacting the sample with an amount of functionalized magnetic particles with a hydrophobic surface, (b) incubating the sample and the particles, thereby adsorbing the compound to the hydrophobic surface, (c) separating the particles by applying a magnetic field and removing the liquid, (d) optionally washing the particles, (e) eluting the compound from the particles.Type: GrantFiled: June 5, 2008Date of Patent: October 19, 2010Assignee: Roche Diagnostics Operations, Inc.Inventors: Uwe Kobold, Albert Geiger, Rupert Herrmann, Michael Vogeser
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Patent number: 7803935Abstract: Biscationic organic compounds are disclosed which promote adsorption of nucleic acids from an aqueous solution to a solid phase such as silica. Adsorption takes place under low salt conditions. Further disclosed are methods and kits suitable for nucleic acid isolation from aqueous solutions.Type: GrantFiled: April 18, 2008Date of Patent: September 28, 2010Assignee: Roche Diagnostics Operations, Inc.Inventor: Christian Birkner
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Publication number: 20100222564Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.Type: ApplicationFiled: November 2, 2009Publication date: September 2, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Richard C. Conrad
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Publication number: 20100222560Abstract: A universal column is provided which allows purification methods utilizing centrifugation, syringe coupling and/or use of a vacuum source. Methods for using the universal column and kits comprising the universal column are described.Type: ApplicationFiled: March 2, 2010Publication date: September 2, 2010Inventor: Xiyu Jia