Extraction Processes (e.g., Solvent Extraction Process, Etc.) Patents (Class 536/25.41)
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Publication number: 20090062522Abstract: The present invention concerns new monolithic shaped bodies, a method for their preparation as well as their use especially for the selective purification and separation of biopolymers. A chromatographic separation material is provided with the new monolithic shaped bodies that allows a selective, efficient and reproducible purification and separation of biopolymers.Type: ApplicationFiled: July 27, 2005Publication date: March 5, 2009Inventors: Christoph Erbacher, Christoph Ritt, Markus Kirchmann
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Publication number: 20090048438Abstract: A method for washing a column is characterised by using a washing liquid, such as an eluting buffer, water, or even any expected buffer, between the traditional washing step and the eluting step as to effectively remove the impurities and/or residual washing liquid in a column from the previous washing step. A method for extracting membrane-bound target molecules containing the additional washing method is also devised.Type: ApplicationFiled: August 13, 2007Publication date: February 19, 2009Inventor: David Daf
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Publication number: 20090048439Abstract: Provided are solid supports that contain at least one hydrophilic ligand; and at least one hydrophobic ligand, where amount of the at least one hydrophobic ligand on the solid support relative to the amount of the at least one hydrophilic ligand on the solid support is adjusted for binding target nucleic acid(s) from a sample onto the solid support and/or for eluting the bound target nucleic acid(s) from the solid support, so that the amount of target nucleic acid(s) bound to the solid support and/or recovered after elution from the solid support is about 5% to about 500% greater than the amount of target nucleic acid(s) bound to the solid support and/or recovered from the solid support in the absence of either the at least one hydrophobic ligand or the at least one hydrophilic ligand or both. The solid supports with ligands are used for isolation of nucleic acid molecules from samples.Type: ApplicationFiled: August 5, 2008Publication date: February 19, 2009Inventors: William G. Weisburg, Elizabeth L. Mather, Marjan Haghnia
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Method for isolation of DNA, RNA and proteins from formalin-fixed paraffin-embedded tissue specimens
Publication number: 20090035761Abstract: Methods are disclosed for rapid, reliable and simple isolation of RNA from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue.Type: ApplicationFiled: July 27, 2007Publication date: February 5, 2009Inventors: Kathleen Danenberg, Peter V. Danenberg, Steven Swenson -
Publication number: 20090036665Abstract: A low-cost, non-instrumented, easy-to-use disposable platform for extraction, stabilization, and preservation of viral RNA in specimens at the point of collection is described. The system may use chemical heating. The platform performs the following steps: specimen lysis, RNA extraction, and RNA stabilization in a modular approach. This modular approach confers versatility to the product for application to multiple targets such as avian flu, and HIV, specimens such as blood, nasal swabs, and downstream applications such as PCR or transcription-mediated amplification. The technology described is a point-of-care specimen-processing platform generically applicable to both emerging point-of-care and central-facility molecular diagnostic tests, as well as to surveillance applications.Type: ApplicationFiled: July 11, 2008Publication date: February 5, 2009Inventors: Gonzalo Domingo, Bernhard Weigl, Paul Labarre, Jay Gerlach
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Patent number: 7482116Abstract: The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention.Type: GrantFiled: June 5, 2003Date of Patent: January 27, 2009Assignee: DNA Genotek Inc.Inventor: H. Chaim Birnboim
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Publication number: 20090018323Abstract: The present invention relates to an improved method for isolating nucleic acids, particularly genomic desoxyribonucleic acid (DNA) from blood.Type: ApplicationFiled: July 25, 2006Publication date: January 15, 2009Applicants: Qiagen GmbH, Qiagen ASInventors: Christoph Erbacher, Ralf Himmelreich, Ralf Peist, Ingerlise Evans Haaland, Hege Hardersen
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Publication number: 20090011417Abstract: The invention provides, in different aspects, a system, sample preparation device, sample processing cartridge, kit, methods of use, business methods, and computer program product.Type: ApplicationFiled: March 7, 2008Publication date: January 8, 2009Inventors: George Maltezos, Axel Scherer, Jingqing Huang, Alex Dickinson, Matthew Johnston
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Publication number: 20080318250Abstract: Methods and compositions for identifying and isolating sperm cells from samples containing multiple cell types are described. The methods and compositions employ antibodies that specifically bind to sperm-specific antigens located on or internal to the sperm plasma membrane. A reporter molecule may be conjugated to the antibodies to aid in the detection of sperm. The antibodies may be targeted to sperm-specific antigens in the head and/or tail of sperm to facilitate the identification and isolation of sperm cells from forensic samples prepared from sexual assault evidence. Purified DNA from the isolated sperm cells can be amplified by polymerase chain reaction to assist forensic analysis in sexual assault cases.Type: ApplicationFiled: February 7, 2005Publication date: December 25, 2008Inventors: Linda Gilmer, Arabinda Mandal, Michael J. Wolkowicz, Kenneth L. Klotz, John C. Herr
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Publication number: 20080300396Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.Type: ApplicationFiled: March 24, 2008Publication date: December 4, 2008Applicant: INVITROGEN CORPORATIONInventors: ARNE Helge DEGGERDAL, Frank Larsen
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Patent number: 7459548Abstract: The invention provides methods for removing a contaminant or inhibitor from a nucleic acid-comprising sample, wherein the contaminant or inhibitor inhibits the amplification or hybridization of the nucleic acid in the sample, or inhibits an enzymatic reaction utilizing the nucleic acid in the sample, the method comprising the steps of: (a) providing a reaction mixture comprising the sample, a chaotropic agent, ammonium acetate or an equivalent, and a detergent, (b) isolating the nucleic acid and remaining contaminants and inhibitors from the reaction mixture in a supernatant; and (c) contacting the nucleic acid supernantant with a flocculant resulting in the further removal of the contaminant or the inhibitor from the supernatant. The invention also provides kits that comprise the components necessary to carry out the method.Type: GrantFiled: May 20, 2005Date of Patent: December 2, 2008Assignee: Mo Bio Laboratories, Inc.Inventors: Mark N. Brolaski, Raveendran J. Venugopal, David Stolow
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Publication number: 20080293931Abstract: An improved method for preparing nucleic acid extracts from environmental samples contaminated by polymerase inhibitors such as humic and fulvic acids is provided. The methods of the invention utilize chemical compositions capable of precipitating humic and/or fulvic acids from organic samples. The methods may be used in connection with the preparation of DNA and RNA extracts, thereby providing purified preparations suitable for amplification using PCR, RT-PCR, and other nucleic acid amplification technologies. Reagent kits for preparing a purified nucleic acid-containing extract from an environmental sample of soil, fluid, or organic particles, using the chemical compositions of the invention are also provided.Type: ApplicationFiled: February 6, 2008Publication date: November 27, 2008Inventors: John M. Dunbar, Peter C. Stark, Thomas W. Robison, Raquel A. Lazarin
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Publication number: 20080287669Abstract: The present invention provides methods, compositions, and kits for identifying compounds useful in nucleic acid purification. The methods of the invention include identifying certain characteristics of organic solvents such as miscibility in water, dielectric constant, and the class of the solvent.Type: ApplicationFiled: May 15, 2008Publication date: November 20, 2008Inventors: Jeffrey C. BRAMAN, Lee Scott Basehore, Natalia Novoradovskaya
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Publication number: 20080281089Abstract: Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container.Type: ApplicationFiled: May 8, 2008Publication date: November 13, 2008Applicant: Applera CorporationInventor: Yingjie Liu
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Publication number: 20080275228Abstract: A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the vaType: ApplicationFiled: March 28, 2005Publication date: November 6, 2008Inventors: Toshihiro Mori, Yoshihiko Makino
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Patent number: 7435810Abstract: A method of fabricating polynucleotide arrays includes dissolving a nucleotide monomer, oligonucleotide, or polynucleotide in a solvent containing ionic liquid and depositing the resulting solution on an array substrate. The method has particular application to fabrication of an addressable array of polynucleotides on a substrate that carries substrate bound moieties each with a hydroxyl group. The process may be repeated at specific locations on the array to elongate the polynucleotide deposited on the array.Type: GrantFiled: December 22, 2004Date of Patent: October 14, 2008Assignee: Agilent Technologies, Inc.Inventors: Joel Myerson, Michel G M Perbost, Douglas J Dellinger, Geraldine F Dellinger
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Publication number: 20080213872Abstract: Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument.Type: ApplicationFiled: February 28, 2008Publication date: September 4, 2008Inventor: John Frederick Regan
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Publication number: 20080214793Abstract: Disclosed herein is a method for extracting a biosubstance from a root of a hair, including the step of using as the hair a hair that has pulling force of at least a predetermined reference value to pull out the hair.Type: ApplicationFiled: February 19, 2008Publication date: September 4, 2008Inventors: Takuro Yamamoto, Tomoteru Abe, Kazuhiro Nakagawa, Shiko Yamashita, Haruhiko Soma, Noriyuki Kishii
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Publication number: 20080213870Abstract: The present invention provides a method for obtaining modified DNA from a biological specimen by obtaining a cell suspension from the specimen, if necessary; passing the cell suspension through a first filter under conditions sufficient to obtain filter-bound cells and suspended DNA; lysing the filter-bound cells under conditions sufficient to release cellular DNA; modifying the DNA bound to the filter under conditions sufficient to release the modified DNA from the filter into a flow-through volume; passing the flow-through volume through a second filter under conditions sufficient to capture the modified DNA to the second filter; and eluting the modified DNA from the second filter.Type: ApplicationFiled: March 3, 2008Publication date: September 4, 2008Inventors: Sean Wuxiong Cao, Jadwiga Katarzyna Markiewicz, George A. Green
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Publication number: 20080206745Abstract: Disclosed are methods and compositions for extracting nucleic acids from a biological sample. In particular, disclosed is a nucleic acid extraction solution together with methods using such a solution for extracting nucleic acid sequences from biological samples containing cells, cellular debris or both. The nucleic acid extraction solution contains a molecule having the formula R1O—CH2—CH2—OR2, wherein R1 and R2 independently are selected from the group consisting of hydrogen and an alkyl group.Type: ApplicationFiled: July 20, 2005Publication date: August 28, 2008Inventors: Brian Lentrichia, Menashi A. Cohenford
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Patent number: 7411061Abstract: A method of synthesizing polynucleotides is disclosed. The method involves contacting a first nucleotide with a selected reactive group in the presence of an ionic liquid. The selected reactive group may be on a second nucleotide, a polynucleotide, or on a moiety on an insoluble substrate, for example in an oligonucleotide synthesizer.Type: GrantFiled: December 22, 2004Date of Patent: August 12, 2008Assignee: Agilent Technologies, Inc.Inventors: Joel Myerson, Michel G. M. Perbost, Douglas J. Dellinger, Geraldine F Dellinger
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Publication number: 20080176209Abstract: Compositions and methods are disclosed for substantially dry storage at ambient temperatures of biological samples such as nucleic acids and cells in a form from which nucleic acids can be recovered, using a dissolvable or dissociable dry storage matrix that permits recovery of biologically active materials. Compositions and methods are also disclosed for automated storing, tracking retrieving and analyzing of nucleic acid samples. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.Type: ApplicationFiled: October 22, 2007Publication date: July 24, 2008Applicant: BIOMATRICA, INC.Inventors: Rolf Muller, Sohela de Rozieres, Sharron Ohgi, Judy Muller-Cohn
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Publication number: 20080166703Abstract: The invention relates to a rapid method for isolating nucleic acid from a nucleic acid source, wherein the nucleic acid source is lysed in the absence of a chaotropic salt and in the absence of an alcohol. The lysate is subsequently filtered through a porous matrix consisting of a material based on silica or of a silica coated material, which binds the nucleic acid in the absence of a chaotropic salt and in the absence of an alcohol. Finally, the nucleic acid is eluted from the porous matrix by an aqueous buffer solution. Furthermore, this invention relates to a test kit in order to isolate the nucleic acid.Type: ApplicationFiled: October 20, 2004Publication date: July 10, 2008Applicant: QIAGEN GmbHInventors: Ralf Himmelreich, Sabine Werner, Claudia Fritz
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Publication number: 20080167258Abstract: A group I intron-derived ribozyme which binds RNA in trans, excises an internal segment from within the RNA, and splices the remaining 5? and 3? ends of the RNA back together (the trans-excision-splicing reaction) is disclosed. The excised segment can be as long as 28 nucleotides, or more, and as little as one nucleotide. The ribozymes of the invention are easily modified to alter their sequence specificity. Such ribozymes represent a new and potentially powerful class of generally adaptable genetic therapeutics.Type: ApplicationFiled: March 20, 2007Publication date: July 10, 2008Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATIONInventors: Stephen M. Testa, Michael A. Bell
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Publication number: 20080168572Abstract: The invention provides a genome of the hyperthermophile Nanoarchaeum equitans, polypeptides, including enzymes, structural protein and binding proteins, derived from this genome, polynucleotides encoding these polypeptides, methods of making and using these polynucleotides and polypeptides. The invention also provides isolated hyperthermophile Nanoarchaeum equitans.Type: ApplicationFiled: May 1, 2003Publication date: July 10, 2008Applicant: Diversa CorporationInventors: Karl O. Stetter, Elizabeth Waters, Keith Kretz, Mircea Podar, Toby Richardson, Michiel Noordewier
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Publication number: 20080160511Abstract: A capture probe suitable for use with a method for isolating miRNAs. A method for isolating an miRNA of interest from a sample comprising the miRNA of interest comprising providing the capture probe. A method for identifying an miRNA of interest.Type: ApplicationFiled: August 18, 2006Publication date: July 3, 2008Inventors: Elliott P. Dawson, Kristie E. Womble
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Publication number: 20080146790Abstract: A system for collection, stabilization, and preparing total DNA for direct purification from biological samples is provided.Type: ApplicationFiled: August 16, 2005Publication date: June 19, 2008Inventors: Daniel Grolz, Christian Lenz, Uwe Oelmuller, Manfred Weiler, Timothy A. Stevens
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Publication number: 20080146791Abstract: The invention relates to an extraction method for isolating target molecules from a sample using a microfluidic carrier.Type: ApplicationFiled: November 28, 2007Publication date: June 19, 2008Inventors: Cord F. Stahler, Peer F. Stahler, Manfred Muller
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Publication number: 20080139800Abstract: The present invention relates to a process for the concentration and/or isolation of nucleic acids or nucleic acid-containing species from a nucleic acid-containing solution, and a kit therefor. In one embodiment, the invention relates to the concentration and/or isolation of DNA and RNA from nucleic acid-containing solutions.Type: ApplicationFiled: June 3, 2004Publication date: June 12, 2008Applicant: QIAGEN GmbHInventors: Arne Deggerdal, Evy H Reitan, Vidar Skagestad, Tine Thorbjornsen
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Publication number: 20080139801Abstract: This invention provides compositions and methods for rapid separation, isolation and purification of nucleopolymers, especially nucleic acids, from biological samples using anionic exchange media. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The medium/nucleoprotein bound complex may be optionally stored or transported, but is subsequently treated with appropriate eluents to remove any undesirable proteins, including destabilizing enzymes, and inorganic salts and the like. The partially purified complex may also be stored or transported prior to further processing.Type: ApplicationFiled: October 10, 2007Publication date: June 12, 2008Inventors: Samuil R. Umansky, Hovsep Melkonyan, Erik Meyer, William John Feaver
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Publication number: 20080132694Abstract: The present invention provides a method for isolating biopolymers, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from an aqueous solution utilizing magnetic particles, particularly silica magnetic particles. The method of the present invention involves forming a complex of the magnetic particles and the biopolymer in a mixture of said particles and said aqueous solution, comprising a salt and an additive; separating the complex from the mixture by a magnetic force, and eluting the biopolymer from the complex, wherein advantageously no substantial clustering of said magnetic particles occurs during the performance of the method. In a preferred embodiment the method of the invention is performed as an automated process.Type: ApplicationFiled: August 16, 2004Publication date: June 5, 2008Inventors: Ralf Himmelreich, Sabine Werner
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Patent number: 7355038Abstract: The present invention relates to a phase isolation process for biomacromolecule components, after pretreating the substance to be isolated: 1) adding sufficient amount of buffer (one of the compound selected from ammonium sulfate, cesium chloride, sodium sulfate, and ethylenediamine tetraacetic acid) and homogenizing gently to carry out agglutination; 2) adding phase isolation solution (one compound or a mixture of more compound selected from the group consisting of ethanol, isopropyl alcohol, isobutyl alcohol and acetone) and homogenizing gently, centrifuging to form an upper phase and a lower phase to carry out phase isolation; wherein lipid is in the upper phase, protein precipitates in the phase middle, DNA plasmid, viral nucleic acid, mitochondrial DNA are in the lower phase, and total RNA precipitates in the lower phase; genomic DNA precipitates in the lower phase or in the phase middle together with protein.Type: GrantFiled: September 24, 2004Date of Patent: April 8, 2008Assignee: V-Gene Biotechnology LimitedInventor: Weiming Yu
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Patent number: 7345163Abstract: Methods for deprotecting and derivatizing oligonucleotides that are non-covalently bound to a solid support are described. The methods include providing a plurality of oligonucleotides linked to one or more hydrophobic separation functions, wherein the plurality includes protected oligonucleotides, precipitating the plurality of oligonucleotides on a hydrophobic solid support using an organic solvent to produce non-covalently immobilized oligonucleotides, and deprotecting the immobilized oligonucleotides.Type: GrantFiled: August 28, 2002Date of Patent: March 18, 2008Assignee: Quiatech ABInventor: Marek Kwiatkowski
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Publication number: 20080032288Abstract: The present invention relates to a temperature-stable labeling reagent of formula (0): in which: R1 represents H or an alkyl, aryl or substituted aryl group, R2 represents a detectable marker or at least two detectable markers interlinked by at least one multimeric structure, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R3 and R4 represent, independently of one another: H, NO2, Cl, Br, F, I, R2 —(L)n—Y—X—, OR, SR, NR2, R, NHCOR, CONHR, COOR, —CO—NH—(CH2)3—(O—CH2—CH2)3-CH2—NH—R2, —CO—NH—(CH2)3—(O—CH2—CH2)4—CH2—NH—R2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, preferably 0 or 1, —Y—X— represents —CONH—, —NHCO—, —CH2O—, —CH2S—, -Z-represents —NH—, —NHCO—, —CONH— or —O—, m is an integer between 1 and 10, preferably between 1 and 3, and p is an integer between 1 and 10, preferably between 1 and 3Type: ApplicationFiled: March 24, 2005Publication date: February 7, 2008Applicant: bioMerieuxInventors: Ali Laayoun, Eloy Bernal-Mendez
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Patent number: 7270983Abstract: This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.Type: GrantFiled: February 3, 2005Date of Patent: September 18, 2007Assignee: Research Foundation of The University of Central Florida, Inc.Inventors: Jack Ballantyne, Jane Juusola
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Patent number: 7264927Abstract: A method for the isolation and purification of nucleic acids such as DNA, RNA, and PNA from various sources using cellulose particles or cellulose paper. Adjusting the concentrations of the salt and polyalkylene glycol to the levels that result in binding of nucleic acids to the cellulose particles or cellulose paper. Separating the nucleic acids bound to the cellulose particles or paper and eluting the nucleic acids from the particles or paper.Type: GrantFiled: September 12, 2002Date of Patent: September 4, 2007Assignee: Cortex Biochem, Inc.Inventors: Ruhangiz D. Nargessi, Matt Pourfarzaneh
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Patent number: 7238530Abstract: The invention relates to methods for easily separating single stranded nucleic acid material from double stranded nucleic acid material in a sample containing both. By the right choice of at lease one chaotropic agent, preferably a guanidine salt, at a selected concentration and other suitable conditions such a chelating agents, pH and the like, it is possible to bind double stranded material to a solid phase such as silica particles, whereas single stranded material will not bind under those circumstances. By separating the silica particles from the sample the double stranded nucleic acid material is removed. It can easily be eluted from the silica particles. In a second step the single stranded material may be bound to a solid phase by selecting a different set of conditions. The particles can again be separated from the sample and the single stranded material may now be eluted. For very efficent separations, the process may be repeated.Type: GrantFiled: January 12, 2001Date of Patent: July 3, 2007Assignee: bioMerieux SAInventors: Jaap Goudsmit, Cornelis Johannes Andreas Sol, Marcellinus Gualbertus Hubertus Maria Beld, Willem René Boom
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Patent number: 7227018Abstract: The object of the present invention is to provide a nucleic acid purification method and purification apparatus characterized by high washing efficiency where contamination does not occur and liquid does not remain in the nozzle tip. The present invention provides a tip containing the solid phase capturing a nucleic acid characterized in that washing solution is fed into said tip unidirectionally from head to end.Type: GrantFiled: December 31, 2003Date of Patent: June 5, 2007Assignee: Hitachi, Ltd.Inventors: Yoshiyuki Shoji, Toshiaki Yokobayashi, Hiroshi Umetsu
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Patent number: 7183407Abstract: The object of the present invention is to provide a nucleic acid purification method and purification apparatus characterized by high washing efficiency where contamination does not occur and liquid does not remain in the nozzle tip. The present invention provides a tip containing the solid phase capturing a nucleic acid characterized in that washing solution is fed into said tip unidirectionally from head to end.Type: GrantFiled: September 15, 2003Date of Patent: February 27, 2007Assignee: Hitachi Ltd.Inventors: Yoshiyuki Shoji, Toshiaki Yokobayashi, Hiroshi Umetsu
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Patent number: 7173124Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.Type: GrantFiled: September 23, 2005Date of Patent: February 6, 2007Assignee: Invitrogen Dynal ASInventors: Arne Helge Deggerdal, Frank Larsen
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Patent number: 7148343Abstract: Reagents, methods and kits for the purification of RNA from biological materials are provided.Type: GrantFiled: April 16, 2003Date of Patent: December 12, 2006Assignee: Gentra Systems, Inc.Inventors: Robert Jackson Bair, Jr., Ellen M. Heath, Heather Meehan, Kim Elayne Paulsen, John M. Wages, Jr.
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Patent number: 7144713Abstract: A suitable carrier molecule useful in a nucleic acid precipitation method is modified by coupling a suitable visualizable indicator molecule to the carrier molecule. The conjugated carrier facilitates nucleic acid precipitation because the presence and location of nucleic acid in a sample is readily observed and monitored.Type: GrantFiled: October 1, 1996Date of Patent: December 5, 2006Assignee: EMD Biosciences, Inc.Inventor: Mark R. McCormick
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Patent number: 7129344Abstract: A method for isolating plasmids DNA from a DNA containing material which comprises plasmid DNA and genomic DNA, comprising extracting the plasmid DNA into a water-immiscible organic solvent, a chaotrope and water under conditions to denature the genomic DNA and recovering the plasmid DNA from the organic phase.Type: GrantFiled: November 17, 1999Date of Patent: October 31, 2006Assignee: Whatman Bioscience LimitedInventors: Neil James Butt, Christopher Peter Jones
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Patent number: 7119194Abstract: A nucleic acid-bondable magnetic carrier of the present invention is a magnetic silica particle comprising a superparamagnetic metal oxide, wherein the magnetic silica particle has a specific surface of about 100 to about 800 m2/g.Type: GrantFiled: July 22, 2002Date of Patent: October 10, 2006Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroaki Uematsu, Katsuya Daimon, Satoko Yoshiga
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Patent number: 7109322Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural source are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.Type: GrantFiled: September 26, 2002Date of Patent: September 19, 2006Assignee: Qiagen GmbHInventors: Metin Colpan, Peter Moritz, Joachim Schorr
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Patent number: 7078517Abstract: It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.Type: GrantFiled: December 17, 2001Date of Patent: July 18, 2006Assignees: Toyo Kohan Co., LTD, Nihon Parrkerizing Hiroshima Co., LTDInventors: Kojiro Takahashi, Osamu Takai, Michifumi Tanga
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Patent number: 7074917Abstract: A method for efficiently purifying 5? protected 2?-deoxypurine nucleosides, efficient production of which has previously been difficult. Impurities can be separated by obtaining the 5? protected 2?-deoxypurine nucleoside as an inclusion crystal including a solvent such as that having a nitrile structure in order to purify the 5? protected 2?-deoxypurine nucleoside at a high purity. This invention enables synthesis of highly purified, protected deoxypurine nucleosides easily on a large scale, which has previously been performed by column chromatography method.Type: GrantFiled: August 16, 2005Date of Patent: July 11, 2006Assignee: Mitsui Chemicals, Inc.Inventors: Hironori Komatsu, Toshiyuki Kouno, Katsutoshi Tsuchiya
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Patent number: 7022835Abstract: The invention concerns a method for binding nucleic acids to a solid phase in which a nucleic acid containing solution is contacted with a solid phase which has hydrophobic and hydrophilic groups on its surface or/and which comprises a hydrophilic, water-containing polymer matrix, in the presence of dehydrating reagents, in particular in the presence of a salt and polyethylene glycol whereby the nucleic acid is bound to the surface.Type: GrantFiled: September 8, 2000Date of Patent: April 4, 2006Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften. E.V.Inventors: Holger Rauth, Richard Reinhardt, Eckard Nordhoff
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Patent number: 6992182Abstract: The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from biological materials, in particular stool samples which may contain contaminations and interfering substances. Furthermore, a reagent kit suitable for carrying out the method of the invention is described.Type: GrantFiled: January 5, 2000Date of Patent: January 31, 2006Assignee: Qiagen GmbHInventors: Oliver Müller, Markus Sprenger-Haussels, Helge Bastian, Stefanie Vollert
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Patent number: 6969603Abstract: Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.Type: GrantFiled: October 31, 2001Date of Patent: November 29, 2005Assignees: Riken, Kabushiki Kaisha DnaformInventors: Yoshihide Hayashizaki, Piero Carninci