Abstract: Fluid access devices include a machine-side hydraulic circuit and a patient-side hydraulic circuit, and are configurable between a connected state and at least one disconnected state. In the connected state, fluid flows between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In the disconnected state, fluid does not flow between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In some disconnected states, fluid recirculates through at least one of the machine-side hydraulic circuit or the patient-side hydraulic circuit in the disconnected state.

Abstract: An example method includes generating an acoustic ultrasound wave that is focused at a focal point. The method further includes sequentially directing the focal point upon distinct portions of an object to form respective shock waves at the distinct portions of the object. The method further includes, via the respective shock waves, causing the distinct portions of the object to boil and form respective vapor cavities. The method further includes causing substantially uniform ablation of a region of the object that comprises the distinct portions. The substantially uniform ablation is caused via interaction of the respective shock waves with the respective vapor cavities. An example ablation system and an example non-transitory computer-readable medium, both related to the example method, are also disclosed.

Abstract: An open channel microfluidic device for use in tissue culture is described. The open channel microfluidic device is used as a patterning rail in a cell culture system to create suspended tissues with cells suspended in a hydrogel. The present disclosure also provides methods of preparing a tissue using the open channel microfluidic device described herein.

Abstract: A continuous reactor and method for destroying contaminants, such as perfluoroalkyl and/or polyfluoroalkyl substances in various feedstocks. Liquid byproducts are continuously hydrolyzed in an aqueous alkaline solution to achieve greater than 99.99% destruction of the contaminants. Continuous hydrolysis achieves a greater conversion efficiency as compared to batch reactions and has a wide application of contaminated feedstocks.

Abstract: Providing an auxin derivative that can exert its intended effect more efficiently, while reducing any unintended effects. A compound represented by the General Formula (1) having a specific substituent at the 5- and/or 6-position of the auxin indole ring.

Abstract: The present disclosure relates to a method for preparing a DNA sequencing library that brings more distal fragmentation breakpoints into close proximity to the non-degenerate sequence tag. In an embodiment, the method comprises circularizing a target fragment library with a plurality of adaptor molecules to produce a population of first circularized double-stranded DNA molecules, wherein the plurality of adaptor molecules comprises a first defined sequence P1 comprising a first restriction enzyme recognition site R1, a degenerate sequence tag, and a second defined sequence P2 comprising a second restriction enzyme recognition site R2, such that at least one of the first circularized double-stranded DNA molecule comprises a non-degenerate sequence tag and a member of the target fragment library. The method is useful because some sequencing platforms perform optimally with template molecules that are relatively short, for example, less than about 500 base pairs in length.

Abstract: Provided herein are biomarkers, methods of diagnosing, methods of treatment, methods of monitoring treatment, and methods of selecting treatment in a subject suspected of, or suffering from, Pancreatic cancer. In some embodiments, the methods disclosed herein comprise determining expression levels of at least one biomarker in one or more biological sample obtained from the subject, and comparing the expression levels of the at least one biomarker in the one or more biological sample obtained from the subject with an expression level of the at least one biomarker in a reference/control sample. In some embodiments, the at least one biomarker comprises High mobility Group A2 (HMGA2). In some embodiments, the pancreatic cancer is Pancreatic Ductal Adenocarcinoma (PDA). Also provided herein are reagents, compositions, and kits for the detection, diagnosis, and prognosis of pancreatic cancer.

Abstract: An implantable patient-controlled analgesic device includes a microelectrode, a control member, and a connections member. The microelectrode comprises a conductive polymer doped with a dopant, wherein the microelectrode is configured to be implanted within a patient's body. The control member is configured to control the microelectrode via an electrical current. The connection member is coupled to the microelectrode and the control member such that the electrical current can flow from the control member to the microelectrode. In some instances, the implantable patient-controlled analgesic device can be coupled to a surgical drain.

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

Abstract: The present disclosure provides methods and compositions useful for generating cellular models of VWF disease from human pluripotent stem cells. In one aspect, the present disclosure relates to a method for the generation of an in vitro cellular model of VWF disease comprising VWF disease-relevant endothelial cells derived from human pluripotent stem cells (hPSCs). In an embodiment, the human pluripotent stem cells (hPSCs) are treated with an agent effective in suppressing or deleting at least one gene sequence encoding a protein and/or a subunit thereof, where the protein and/or subunit thereof is associated with VWF-linked secretion. The present disclosure also provides uses of the cellular models disclosed herein.

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

Abstract: Methods of fabricating a hydrogel tube, and related systems, employ extrusion of a cross-linkable hydrogel solution from an annular outer nozzle of a nozzle assembly to form a cross-linkable hydrogel tube. The cross-linkable hydrogel tube is cured to form a cross-linked hydrogel tube. A second hydrogel solution can be coextruded via the axial inner nozzle to form an inner hydrogel filament coaxially positioned within the cross-linkable hydrogel tube. The cross-linked hydrogel tube can be functionalized with collagen to enable cell adhesion, and cells can be cultured on the luminal surfaces of these tubes to yield tubular endothelial layers. A 3D printed coaxial nozzle can be used to fabricate biofunctional tubular conduits that can be utilized for engineering in vitro models of tubular biological structures.

Abstract: Example systems may include an electronic device (e.g., a smartphone) and an attachment member that may couple the electronic device to a cup. The cup may be positioned within a field of view (e.g., within a focal length) of a camera that is coupled to or integral to the electronic device. The cup may receive a sample of whole blood and/or plasma. The attachment member may convey vibration from the electronic device to the cup. The cup may include a particle positioned in the cup. The camera may capture images of the cup including the particle, and motion of the particle may be used by the electronic device to calculate a clotting time of the sample. For example, the particle may move freely responsive to vibration during a time the sample is in a non-clotted state, but may slow and/or stop motion when the sample has clotted.

Abstract: Devices, systems, methods, and kits configured to perform bioassays on live, intact tissue for precision bioanalysis in vitro are described. These approaches enable precision oncology by capturing determinants of therapeutic response to functional drug testing, such as viability and molecular signal generation, that can depend on tissue architecture, tumor heterogeneity, and the tumor microenvironment. The disclosure provides electrochemical aptamer sensors integrated into arrays of microfluidic traps for the analysis of micro-dissected tumors, as an example. The sensors are utilized with an example of periodic monitoring of cytochrome c (Cyt-C), a soluble cell death indicator, released by micro-dissected tumors.

Abstract: Methods for making an intercalated layered film, intercalated layered films, and devices that include the intercalated layered films. In the method, in a first step, a suspension of a dispersed two-dimensional compound in a fluid is filtered through a filtration medium to provide a layered film of the two-dimensional compound on the filtration medium, and in a second step, filtering a solution of an intercalant in a solvent through the layered film of the two-dimensional compound on the filtration medium to provide the intercalated layered film.

Abstract: Systems and methods to remove carbon from liquids such as seawater and other natural waters are described. The systems and methods utilize photoactive compounds to alter the pH of a fluid, drawing carbon out of the liquid and channeling it into a secondary environment. The carbon can be captured and sequestered or used in the formation of a product.

Abstract: Techniques for concentrating analytes in fluid samples are described. An example method performed by a concentrator includes receiving a fluid sample; concentrating an analyte in the fluid sample by extracting water from the fluid sample; and based on concentrating the analyte in the fluid sample, outputting the fluid sample. The analyte is concentrated by extracting the water through a membrane and into a solution including at least one solute at a concentration that is greater than a concentration of at least one solute in the fluid sample, diameters of pores extending through the membrane being shorter than a diameter of the analyte and shorter than a diameter of the at least one solute.

Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

Abstract: Embodiments of the present disclosure provide systems and methods directed to directional audio source separation. An example method comprising: receiving a plurality of input signals by a plurality of microphones: generating a plurality of beamformed signals based on the plurality of input signals: providing the plurality of beamformed signals and the plurality of input signals to a neural network, wherein the neural network is trained to generate directional signals based on sample beamformed signals and sample input signals: generating an output directional signal using the neural network based on the plurality of input signals and the plurality of beamformed signals: and providing the directional signal to a speaker.

Abstract: Embodiments of the present disclosure provide composition and methods for recording an iterative nucleic acid editing event. The compositions and methods described herein comprise a first active target domain, comprising an editable recording sequence configured to hybridize with a first prime editing guide RNA (pegRNA) and one or more inactive truncated target domains comprising a non-editable sequence configured to not hybridize with the pegRNA, wherein the first pegRNA edits the first active target domain, wherein the pegRNA edit shifts the position of the recoding sequence from the editable sequence to the non-editable sequence, thereby changing the editable sequence to a non-editable sequence and the inactive truncated target domain to a second active target domain comprising a second recoding sequence configured to hybridize with a second pegRNA.