Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

Abstract: Enzyme-loaded polymeric nanoparticles for the treatment of disorders such as neurological or non-neurological conditions are described. Using hydrophobic ion pairing, enzymes are loaded into polymeric nanoparticles while retaining enzymatic activity. Surfactants coating the nanoparticle can direct nanoparticles for cellular uptake or for aggregation in the extracellular matrix. Preparing a hydrophobic ion pairing complex for nanoparticle formation.

Abstract: Systems and methods for deep brain stimulation using kinematic feedback in accordance with embodiments of the invention are illustrated. One embodiment includes a deep brain stimulation system, including an implantable neurostimulator, a first inertial measurement unit (IMU), a second IMU, and a controller, where the controller is communicatively coupled to the implantable neurostimulator, the first IMU, and the second IMU, and where the controller is configured to obtain kinematic data from the first IMU and the second IMU, identify an abnormal movement event based on the kinematic data, and modify deep brain stimulation provided by the implantable neurostimulator based on the identified abnormal movement event.

Abstract: The disclosure provides example apparatus and methods for automatically adjusting a socket size of a prosthesis. The apparatus includes (a) the prosthesis having a socket configured to receive a limb, (b) a first opening in a socket wall, (c) a first panel aligned with the first opening, (d) a first actuator coupled to the first panel and to the prosthesis, the first actuator is configured to advance and retract the first panel, (e) a first sensor coupled to the socket wall and configured to obtain limb-to-socket gap data, and (f) a processor coupled to the first actuator and the first sensor, wherein the processor is configured (i) to receive the limb-to-socket gap data, (ii) to determine a socket-size adjustment based on the limb-to-socket gap data and a predetermined socket-fit value, (iii) to generate and (iv) to send a command with the socket-size adjustment to the first actuator.

Abstract: Systems and methods of identifying medical disorders in one or more subjects are disclosed herein. In one embodiment, sound is transmitted toward a subject and at least a portion of the sound reflected by the subject and is acquired as echo data. The acquired echo data is used to generate a motion waveform having a plurality of peaks detected therein. At least a portion of the plurality of peaks may be indicative of movement of the subject. One or more medical disorders in the subject can be identified based on, for example, time durations and/or amplitude changes between peaks detected in the motion waveform.

Abstract: Methods of functionalizing activated carbon for selective adsorption of a per- or polyfluoroalkyl substance (PFAS), methods of removing PFAS, and compositions comprising activated carbon; and a molecularly imprinted polymer (MIP) coupled to the activated carbon are described. In the embodiment, the method of functionalizing activated carbon for selective adsorption of a PFAS comprises coordinating a PFAS template with a plurality of functional monomers; polymerizing the plurality of functional monomers coordinated with the PFAS template in the presence of an activated carbon substrate to provide a molecularly imprinted polymer (MIP) coupled to the activated carbon; and extracting the PFAS template from the MIP.

Abstract: Metasurfaces and systems including metasurfaces for imaging and methods of imaging are described. In one embodiment, a method for acquiring images by an imaging system comprising a metalens includes: illuminating the metalens; acquiring light passing through the metalens as a first image by an image sensor; and processing the first image into a second image that is a deconvolved version of the first image by a post-processing engine. The metalens includes a plurality of nanoposts carried by a substrate.

Abstract: The disclosure provides methods of preventing or treating heart failure in a mammalian subject. The methods comprise administering to the subject an effective amount of an aromatic-cationic peptide to subjects in need thereof.

Abstract: The present invention provides methods, processes, and systems for the manufacture of three-dimensional articles made of polymers using 3D printing. A layer of prepolymer is deposited on a build plate to form a powder bed. Then, solutions of first and/or second binding agents are printed on the powder bed in a predetermined pattern. After a predetermined period of time, sequential layers are printed to provide the three-dimensional article. The removable binding agent is then removed. The three-dimensional object can be cured to produce the three-dimensional article composed of the final polymers.

Abstract: Methods of LC-MS/MS quantification of ?-carboxylated proteins in plasma, serum, or blood, including dried blood spots, are disclosed. The methods can be used to determine patient-specific dosing of anticoagulant drugs and diagnosis of liver diseases, such as hepatocellular carcinoma.

Abstract: In some embodiments, a sensor assembly for measuring a depth of insertion of a locking pin of a liner in a pin lock of a lower-limb prosthetic is provided. The sensor assembly comprises a base; a sensing element mounted to the base and having a hollow interior; and a housing that encloses at least the sensing element and includes means for coupling the sensor assembly to the lower-limb prosthetic in a position where the locking pin adapter of the lower-limb prosthetic is aligned with the hollow interior of the sensing clement. The sensing element is configured to generate a signal indicative of a size of a portion of the locking pin that is within the hollow interior of the sensing element.

Abstract: The current disclosure describes methods and composition for treating and diagnosing tauopathies utilizing transcript variants of PSEN2. Reduction of transcript variants of PSEN2 and/or preservation of canonical splicing can be beneficial in the treatment of tauopathies such as Alzheimer's disease. Methods of diagnosing sporadic Alzheimer's disease are also provided.

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

Abstract: Various implementations described herein relate to the treatment of skin fragility diseases. Example methods and compositions described herein relate to inhibitors of the mitogen-activated protein kinase kinase (MEK or MAPK) and/or the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway for skin fragility disease treatment. According to some implementations, the inhibitor is formulated for topical administration.

Abstract: Embodiments of the present disclosure provide a method for detecting one or more genetic variants in a biological sample. Embodiments of the method include preparing an error-corrected nucleic library for sequencing, wherein the nucleic acid library comprises a double stranded nucleic molecule comprising a hairpin adapter, wherein the hairpin adapter covalently joins each strand of the double stranded nucleic molecule into a single covalently linked duplex strand for self-correction of sequencing errors.

Abstract: Wnt signaling antagonist and agonist compositions and methods for their use are provided.

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

Abstract: Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases.

Abstract: Embodiments of the present disclosure provide methods and compositions for increasing the nitrogen (N) fixation of diazotrophs or acquisition of N for a plant in need thereof. Embodiments of the methods and compositions comprise at least one live endophyte strain, wherein the live endophyte strain is isolated from one or more plants grown in a nutrient-limited and/or water-stressed environment. In some embodiments, the endophyte strain can be administered to a plant, wherein the endophyte strain synergistically increases the nitrogen fixation of the diazotrophic strain associated with the plant. In other embodiments, the diazotrophic strain is not associated with a plant. Embodiments of the present disclosure have broad application to reduce fertilizer requirements, increase plant carbon sequestration, increase production of hydrogen gas for use as an energy source or in chemical industries and to increase growth of industrial microbial strains, reducing the need for ammonium or nitrates in fermenters.