Abstract: A method for promoting conversion of cells into cardiomyocytic tissue is carried out by contacting fibrotic tissue (e.g., scar tissue) with a microRNA oligonucleotide or combination of microRNA oligonucleotides. The methods lead to direct reprogramming of fibroblasts to cardiomyocytes or cardiomyoblasts.

Abstract: Angelman Syndrome (AS) is a genetic disorder occurring in approximately one in every 15,000 births. It is characterized by severe mental retardation, seizures, difficulty speaking and ataxia. The gene responsible for AS was discovered to be UBE3A and encodes for E6-AP, an ubiquitin ligase. A unique feature of this gene is that it undergoes maternal imprinting in a neuron-specific manner. In the majority of AS cases, there is a mutation or deletion in the maternally inherited UBE3A gene, although other cases are the result of uniparental disomy or mismethylation of the maternal gene. While most human disorders characterized by severe mental retardation involve abnormalities in brain structure, no gross anatomical changes are associated with AS. We have generated a Ube3a protein with additional sequences that should allow the secretion from cells and uptake by neighboring neuronal cells. This would confer a functional E6-AP protein into the neurons and rescue disease pathology.

Abstract: A system for identifying and evaluating a pocket of a protein includes performing a Voronoi tessellation and developing a Voronoi diagram of a surface of the protein. All alpha-spheres on the surface of the protein are identified. The alpha-spheres are filtered based on radius and remaining alpha-spheres are clustered into alpha-clusters. At least one alpha-cluster is selected for quantitative evaluation. Alpha-sphere contact atoms are determined for a plurality of interaction points of the pocket. A Delaunay triangulation of the four contact atoms of each interaction point is performed. A plurality of alpha-spaces for each interaction point are determined. An alpha-atom and an alpha-atom contact surface area (ACSA) of each interaction point is determined. The pocket is ranked, a pocket-fragment complementarity is determined, and the pocket is matched between various conformations of the proteins.

Abstract: Various embodiments of a system and method for controlling the shape, subdivision, recombination, movement and object manipulation of ferrofluid material in addition to pumping fluids with ferrofluid material using external electromagnetic fields are disclosed herein.

Abstract: A method of designing at least one coil for producing a magnetic field is disclosed. The method comprises: i) setting a performance target comprising: a target magnetic field, and at least two of a target power, a target resistance, a target size and/or weight, a target supply voltage or current, and a target inductance; ii) determining initial design parameters for the at least one coil; iii) modelling performance with the current design parameters to determine a simulated performance against each of the performance targets; iv) calculating a penalty function based on the difference between the simulated performance and the performance targets; v) modifying the design parameters in order to reduce the penalty function; vi) iterating steps iii) to v) until the penalty function or simulated performance has met an acceptance condition.

Abstract: This application relates to a method for optimizing algorithm performance using precision scaling, wherein the method according to an embodiment of present invention comprises obtaining a number of iterations of a unit operation according to precisions of the algorithm including the unit operation that is repeatedly performed, wherein the precisions include a first precision and a second precision, and the number of iterations include a first number of iterations corresponding to the first precision and a second number of iterations corresponding to the second precision; inspecting available precisions of a device on which the algorithm is to be executed, wherein the available precisions include a first available precision corresponding to the first precision and a second available precision corresponding to the second precision; determining an optimal precision by repeatedly performing the unit operation corresponding to an initial operation of the algorithm using the inspected available precision; and repea

Abstract: Described herein are trehalose analogues. Also described herein are methods of making the trehalose analogues and uses of the analogues. For example, the disclosed trehalose analogues may be useful in the detection of bacteria.

Abstract: Compositions and regimens useful in treating type I citrullenemia are provided. The compositions include recombinant adeno-associated virus (rAAV) with a transthyretin enhancer and promoter driving expression of a human Argininosuccinate Synthase 1 (ASS1).

Abstract: The present invention discloses an ion beam lithography method based on an ion beam lithography system. The ion beam lithography system includes a roll-roll printer placed in a vacuum, and a medium-high-energy wide-range ion source, a medium-low-energy wide-range ion source and a low-energy ion source installed on the roll-roll printer. The ion beam lithography method includes: first coating a polyimide (PI) substrate with a dry film, etching the dry film according to a preset circuit pattern, then using the ion beam lithography system to deposit a wide-energy-range metal ion on the circuit pattern to form a film substrate, and finally stripping the dry film off the film substrate to obtain a printed circuit board (PCB).

Abstract: A two-stage energy dissipation type shed tunnel support structure connected by a principle of Dougong and a design method thereof are provided. The two-stage energy dissipation type shed tunnel support structure includes a Dougong joint domain, crossbeams and columns. The Dougong joint domain includes section steel members, wavy-wall cylindrical elastoplastic buffers, U-shaped sliding connecting troughs and high-strength bolts. Multiple layers of the section steel members are orthogonally stacked to form a Dougong shape, the wavy-wall cylindrical elastoplastic buffers are arranged between adjacent layers of the section steel members, and the U-shaped sliding connecting troughs are arranged at upper and lower ends of the wavy-wall cylindrical elastoplastic buffers, which realizes a connection between the wavy-wall cylindrical elastoplastic buffers and a section steel in two orthogonal directions.

Abstract: A microfluidic device for non-invasively and passively accessing interstitial fluid from a patient includes a substrate containing multiple vertical micro channels therethrough, wherein at a first end of each of the multiple vertical micro channels a microheater is formed for controllably ablating a portion of dry dead skin cells to access the interstitial fluid; and wherein at a second end of each of the multiple vertical micro channels is a horizontal micro channel for receiving accessed interstitial fluid from a vertical micro channel and guiding the accessed interstitial fluid to a common collection port.

Abstract: Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the method includes generating nucleosome-depleted nuclei by chemical treatment while maintaining integrity of the nuclei. Also provided herein are compositions, such as compositions that include chemically treated nucleosome-depleted isolated nuclei.

Abstract: A method for localizing a target object may include applying, to a first image captured by an apparatus, a first machine learning model trained to determine whether the target object is visible in the first image depicting a first region of a search area. In response to the target object being absent from the first image, a second machine learning model may be applied to identify, based on the target object as being visible and/or nonvisible in one or more portions of the first image, a second region of the search area corresponding to a sub-region within the first region of the search area having a highest probability of including the target object. A command may be sent to the apparatus to navigate the apparatus to the second region of the search area in order to capture a second image of the second region of the search area.

Abstract: The present invention discloses a Fire-fighting water cannon comprising: a first motor assembly including a first housing, a first stator assembly, a first rotor assembly and a central shaft; both ends of the first housing are respectively provided with a first end cover and a second end cover, the first end of the first stator assembly is connected with the second end cover, the first rotor assembly is sleeved on the outside of the first stator assembly, and the first rotor assembly is rotatably connected with the first housing; the cannon head assembly including a first cannon head and a second cannon head, the first cannon head is connected with the first stator assembly, the second cannon head is sleeved on the outside of the first cannon head, and the second cannon head is threadedly connected with the first rotor assembly, the first cannon head is provided with a first anti-rotation device; the cannon core assembly is arranged on the central axis and cooperates with the cannon head assembly.

Abstract: Provided are a computer mouse and a method for operating the computer mouse, with the computer mouse including a palm-rest on a first side thereof, a baseplate on a second side substantially opposite the first side, hinges positioned along an edge of the palm-rest, and finger-rests, with each finger-rest fixedly attached to one end of a respective hinge.

Abstract: A ride vehicle system includes a ride vehicle including a seat configured to accommodate a passenger, a restraint system of the ride vehicle, where the restraint system includes a restraint configured to secure the passenger within the seat, and a forced air system of the ride vehicle, where the force air system is configured to generate an air flow, direct the air flow internally through the restraint, and discharge the air flow toward the passenger via a discharge port of the restraint.

Abstract: An apparatus for providing a customized mobility driving path using a brain wave signal includes a sensor configured to collect a brain wave signal for a driver of a mobility in a predetermined channel region, an analyzer configured to determine information to be provided regarding a planned path by analyzing the brain wave signal collected in the predetermined channel region, and a controller configured to control an operation of the mobility based on the information to be provided.

Abstract: The present invention discloses a method for proppant suspension and suspension parameter optimization based on bubble bridge effect, comprising: select a proppant and hydrophobically modify its surface to obtain a hydrophobically surface-modified proppant; prepare a bubbly fracturing base fluid; make the first optimization of the base fluids according to the average radius of the proppant and the average radius of the bubbles of the base fluids; optimally select the base fluids selected for the second time according to the interaction energy between the proppant particle and the bubble after the hydrophobically surface-modified proppant mixed with the base fluid; the basic parameters of the bubbly fracturing base fluid selected at the third time were used for the perfect selection for proppant suspension. The present invention establishes a procedure on experimental evaluation and parameter calculation optimization by suspending fracturing proppant with the bubble bridge effect on the hydrophobic surface.

Abstract: The present disclosure provides compositions comprising a lyn kinase activator and TRPM8 agonist, and to methods of: reducing blood glucose levels, weight gain, or fat depot levels; treating metabolic syndrome, Syndrome X, obesity, prediabetes, type II diabetes, type I diabetes; treating hypercholesterolemia, hypertension, coronary heart disease, diabetic neuropathy, lipodystrophy, diabetic retinopathy, erectile dysfunction, kidney disease, dyslipidemia, dyslipoproteinemia, a peroxisome proliferator activated receptor-associated disorder, septicemia, a thrombotic disorder, or pancreatitis; inducing the beiging of adipocytes; and preventing pancreatic beta cell degeneration.

Abstract: Methods for automated segmentation system for retinal blood vessels from optical coherence tomography angiography images include a preprocessing stage, an initial segmentation stage, and a refining stage. Application of machine-learning techniques to segmented images allow for automated diagnosis of retinovascular diseases, such as diabetic retinopathy.