Abstract: The invention relates generally to a method of detecting and/or analysing target polymers, especially target polynucleotides, using a biological pore. The invention also relates to a novel system for carrying out the method. The method has many uses. In particular, the method may be used for diagnosis, detection of polymorphisms and V(D)J repertoire analysis.

Abstract: The synthesis, structure, and reactivity of a family of iridium-based molecular cluster complexes, including neutron and X-ray single-crystal diffraction measurements, are provided. It is found that one complex, which includes an Ir3H6(?3—H) trinuclear core, exhibits reactivity for reversible CO2 hydrogenation via hydride transfer. This reactivity is unlike previous reports of one known Ir3H6(?3—H) complex, which is inert.

Abstract: The disclosure belongs to the technical field of biomedical materials, and provides a bio-ink, application thereof, a penile corpus cavernosum prosthesis and a preparation method thereof. The bio-ink provided by the disclosure includes a polymer-based material, an initiator, a crosslinking agent, a light absorber, and water. The bio-ink provided by the disclosure can undergo a gelation reaction under ultraviolet light irradiation, and an obtained hydrogel matrix material can have an elongation at break reaching 200-600%, so as to be applied to 3D printing. The disclosure further provides a penile corpus cavernosum prosthesis. For the penile corpus cavernosum prosthesis provided by the disclosure, with biocompatible hydrogel as a matrix material, an anticoagulant layer obtained by coating can effectively reduce the risk of thrombosis and has good safety and stability. The penile corpus cavernosum prosthesis of the disclosure has bionic vascular sinus cavity and bionic white membrane structures.

Abstract: A liquid sample collection system is provided. The system includes a housing having a first layer and a second layer coupled to the first layer, one or more channels integrated into the first layer from a central region of the first layer to an edge of the first layer, and a card secured between the first layer and the second layer. The first layer is made, at least in part, from a hydrophobic polymer and the second layer is made, at least in part, from a hydrophilic polymer, and the one or more channels distribute the liquid sample on the card.

Abstract: This document describes a virtual sensor module for generating spatial-temporal temperature measurements of a battery and a method of using the virtual sensor module to generate the above-mentioned measurements. The virtual sensor module utilizes a Proportional-Integral-Derivative (PID) controller module communicatively coupled to a trained convolutional neural network—long-short-term memory (CNN-LSTM) module to trigger the trained CNN-LSTM module to generate a refined predicted series of real temperature sequences across the battery, based on a set of optimized PID parameters (K*p, K*i, K*d), predicted error sequences, and measured temperature sequences of the battery.

Abstract: Disclosed are a hollow hydrogel suture for bidirectional liquid delivery and sensing communication, and preparation methods thereof, which belong to the technical field of biomedicine. The hollow hydrogel suture includes a hydrogel suture body and a compound with a sensing communication function doped in the hydrogel suture body, wherein the compound with the sensing communication function is at least one of polypyrrole and copper-doped zinc sulfide, and the hydrogel suture body is made of polyvinyl alcohol.

Abstract: Disclosed herein are methods for treating impairment of a limb in a subject. Particular methods comprise applying a therapeutically effective amount of an electrical stimulus to dorsal roots, dorsal rootlets, or dorsal root ganglia, of sensory neurons innervating the limb of the subject, wherein the impairment is a motor impairment and/or a proprioception impairment due to neurological disorder or injury, the electrical stimulus is applied with one or more electrodes controlled by a neurostimulator, and the one or more electrodes are implanted in the epidural space at the dorsolateral aspect of the spinal cord and proximate to the dorsal roots or dorsal rootlets of the sensory neurons innervating the limb of the subject, or the one or more electrodes are implanted proximate to dorsal root ganglia of sensory neurons innervating the limb of the subject.

Abstract: Methods and systems for treatment prioritization using a neural network model are provided. Training the model includes generating an enriched genetic knowledge graph with edges characterizing relationships between genetic variants, genes, diseases, treatments and symptoms, embedding the graph vertices in genetic knowledge vectors, receiving training patient feature value sets, generating a training dataset of training patient vectors labelled with target values comprising treatment efficacity by scaling the genetic knowledge vectors by the patient feature value sets, and training the model to process an input patient vector and generate a predicted efficacity of the at least one treatment option.

Abstract: A system and method for determining position of a steerable assembly within tissue of an animal body utilizes an elongated body structure with an implement arranged at a distal end thereof, and a premagnetized material proximate to the distal end. A signal indicative of a length of insertion of the elongated body structure into the tissue is used with a signal indicative of (i) force, strain, shape of a sensor associated with the elongated body structure and/or (ii) directionality of magnetic field applied to the premagnetized material, to determine a three-dimensional (3D) trajectory of the steerable assembly. The 3D trajectory is superimposed on a 3D model of the tissue to determine position of the steerable assembly within the tissue.

Abstract: Fluid access devices include a machine-side hydraulic circuit and a patient-side hydraulic circuit, and are configurable between a connected state and at least one disconnected state. In the connected state, fluid flows between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In the disconnected state, fluid does not flow between the machine-side hydraulic circuit and the patient-side hydraulic circuit. In some disconnected states, fluid recirculates through at least one of the machine-side hydraulic circuit or the patient-side hydraulic circuit in the disconnected state.

Abstract: The present disclosure provides aqueous electrolytes with a lower salt concentration and wider electrochemical stability window compared to water-in-salt electrolytes. The aqueous electrolyte composition of the disclosure comprises an anti-solvent, which reduces the H2O activity and the amount of H2O in the Li-ion solvation sheath. In one particular embodiment of the disclosure provides a nonflammable aqueous electrolyte composition for a lithium-ion battery, wherein the aqueous electrolyte composition comprises: an electrolyte salt comprising a lithium salt; water; and an organic component that is miscible with water. In some embodiments, the electrochemical stability window of the aqueous electrolyte composition is greater than 3.0 V and a molality of an electrolyte salt in said aqueous electrolyte composition is about 5 m or less.

Abstract: The present disclosure relates generally to dis-arming immune cell product sensitivity to androgens. Provided herein are methods, systems, and compositions for disarming androgen receptor (AR) activity in immune cells, for instance in the context of CAR-T cell therapy, allogenic T cell therapy, DC vaccines, macrophage therapy, myeloid/macrophage antigen receptor therapy, NK cell therapy, and so forth. Different methods for reducing AR function are described.

Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.

Abstract: A method of treating a cancer which is characterized by an up-regulation of expression of lysyl oxidase (LOX) and heat shock protein 70 (HSP70) is disclosed. The method comprises administering to the subject a therapeutically effective amount of a polypeptide comprising a propeptide of lysyl oxidase (LOX), said polypeptide being devoid of LOX catalytic activity, wherein said polypeptide binds to both LOX and heat shock protein 70 (HSP70) with a EC50 of less than 100 nM.

Abstract: A method for preparing a self-supporting composite nanofiltration membrane is provided. A porous graphene-based two-dimensional sheet material is prepared by taking amino graphene quantum dots as the main body and subjecting them to an interfacial polymerization reaction with polyacyl chloride, and then the porous graphene-based two-dimensional sheet material is encapsulated in-situ with polyamide by an in-situ encapsulating technology to prepare a self-supporting porous graphene/polyamide separation layer with excellent permeability and high selectivity.

Abstract: Disclosed is a method for producing regulatory T cells, the method comprising: (1) differentiating cells that can differentiate into regulatory T cells, into which an expression construct is introduced, into regulatory T cells, the expression construct comprising: (a) conserved non-coding sequence (CNS) 1, CNS2, and CNS3 of Foxp3 gene; (b) a promoter; and (c) a nucleic acid encoding FOXP3. Also disclosed are regulatory T cells obtained by the method, and a pharmaceutical composition comprising the regulatory T cells.

Abstract: An Internet-of-Things (IoT) learning framework (IoT learning framework) may train AI models to infer user activities from IoT connected device events using machine learning based algorithms. According to such an example, processing circuitry obtains a training dataset indicating IoT device events and extracts representative user activity patterns from the sequences of IoT device events. In such an example, processing circuitry trains the AI model to learn an optimal subset of the sequences of IoT device events corresponding to a smallest quantity of the sequences of IoT device events to predict user activities with accuracy that satisfies a threshold and outputs the AI model. According to such an example, processing circuitry may obtain new data indicating new sequences of IoT device events and generates output indicating one or more user activities predicted by the AI model to have occurred based on the new sequences of IoT device events.

Abstract: Mammalian milk oligosaccharides (MMO) are produced in plants engineered to express recombinant MMO biosynthetic pathways.

Abstract: The present invention relates to a dental zirconia material of which the surface is modified by plasma treatment, an implant, and a method for preparing same. The zirconia material and the implant prepared according to the present invention have improved cell adhesion and anti-bacterial effect, and thus can exhibit improved biocompatibility and minimize an inflammatory response of a user and the like.

Abstract: A membrane filtration cell is provided which includes a fluid passageway and a filtration membrane positioned within the passageway, the filtration membrane dividing the fluid passageway into two chambers, a retentate chamber and a permeate chamber. A first electrode is positioned in the retentate chamber and a second electrode is positioned in the permeate chamber, where the first electrode and the second electrode are configured to apply an electric field across the filtration membrane. The membrane filtration cell also includes an acoustic device configured to apply an acoustic field across the retentate chamber, where the synergistic combination of the electric field and the acoustic field prevents fouling on the filtration membrane.