Capillary Electrophoresis Patents (Class 204/451)
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Patent number: 8721858Abstract: A novel method for visualizing electrokinetic process zones (e.g., for isotachophoresis (ITP)) is provided. We introduce negligibly small concentrations of a fluorophore that is not focused by isotachophoresis. This non-focusing tracer (NFT) migrates through multiple isotachophoresis zones. As it enters each zone, the NFT concentration adapts to the local electric field in each zone. ITP zones can then be visualized with a point detector or camera. The method can be used to detect, identify, and quantify unknown analyte zones, and can visualize complex and even transient electrophoresis processes. This visualization technique is particularly suited to microfluidic and lab-on-a-chip applications, as typical fluorescence microscopes and CCD cameras can provide high-resolution spatiotemporal data.Type: GrantFiled: March 14, 2011Date of Patent: May 13, 2014Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Robert D. Chambers, Juan G. Santiago, Moran Bercovici
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Patent number: 8716025Abstract: A control device for a sample separation apparatus, the sample separation apparatus including a first separation unit and a second separation unit downstream of the first separation unit and supplied with the fluidic sample after treatment by the first separation unit. A control device is configured for controlling the first separation unit to execute a primary separation sequence within a time interval for separating the fluidic sample into fractions, and for controlling the second separation unit to execute secondary separation sequences within the time interval for further separating the separated fractions into sub-fractions, wherein the secondary separation sequences form part of a common sample separation method defined by a common specification of the sample separation involving a set of parameters, and adjusting, over a progress of the primary separation sequence, at least one parameter according to which at least one of the plurality of secondary separation sequences is executed.Type: GrantFiled: July 8, 2011Date of Patent: May 6, 2014Assignee: Agilent Technologies, Inc.Inventor: Klaus Witt
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Publication number: 20140116882Abstract: A microfluidic dielectrophoresis system includes: one supply device for a liquid medium having particles contained therein, N?2 microfluidic, dielectrophoretically active channels, which are equipped with electrodes, lines for the fluidic connection of the supply device to the channels, for the connection of the channels to one another, and for the drainage of the medium and/or the particles from the channels, and valves for setting the flow direction of the medium in the lines, the dielectrophoretically active channels being situated and being connected by lines in such a way that they may be operated connected in parallel and in series by switching the valves in relation to the flow direction of the medium and the electrodes of the various channels are activatable independently of one another.Type: ApplicationFiled: January 6, 2014Publication date: May 1, 2014Applicant: ROBERT BOSCH GMBHInventor: Christian DORRER
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Publication number: 20140116881Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: ApplicationFiled: October 22, 2013Publication date: May 1, 2014Applicant: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
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Publication number: 20140110258Abstract: Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device. The receptors are selected based on their affinity for classes of known toxic agents. The receptors are freed and the bound and unbound receptors separated based on differential electrokinetic mobilities while they travel to a detection device.Type: ApplicationFiled: May 10, 2011Publication date: April 24, 2014Applicant: STC.UNMInventors: Gabriel Lopez, Linnea Ista, Steven RJ Brueck, Aurelio Evangelista Lara, Mangesh Bore
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Patent number: 8702941Abstract: A method for analyzing hemoglobin by electrophoresis, capable of analyzing hemoglobin A1c (HbA1c) and modified hemoglobin with improved accuracy in a shortened analysis time is provided. The method for analyzing hemoglobin by electrophoresis includes performing electrophoresis under conditions in which an acidic substance having two or more carboxyl groups is present in an electrophoresis solution. At least two of the carboxyl groups of the acidic substance each have an acid dissociation constant (pKa) lower than the pH of the electrophoresis solution at the time of analysis.Type: GrantFiled: December 23, 2010Date of Patent: April 22, 2014Assignee: ARKARY, Inc.Inventors: Yusuke Nakayama, Satoshi Yonehara
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Patent number: 8702949Abstract: The solution reservoir apparatus of the capillary electrophoresis apparatus securely affixes an evaporation-preventing membrane to a container when a capillary is inserted or withdrawn, without extending the cathode end of the capillary. The solution reservoir apparatus comprises a container for reserving a sample or solution, a cover having a bore through which the capillary is passed and covering the container, an evaporation-preventing membrane having a capillary hole through which the capillary is passed, and a container holder for holding the container. The evaporation-preventing membrane has a projection provided at the periphery of the capillary hole, the projection of the evaporation-preventing membrane is engaged with the bore on the cover when the evaporation-preventing membrane is positioned on the cover, and the evaporation-preventing membrane is supported by the cover.Type: GrantFiled: April 8, 2010Date of Patent: April 22, 2014Assignee: Hitachi High-Technologies CorporationInventors: Takeshi Ohura, Ryoji Inaba, Manabu Akiba, Takashi Gomi, Mari Kotoura
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Publication number: 20140102900Abstract: In one aspect, single-sided microfluidic devices are described herein. In some embodiments, a single-sided microfluidic device comprises a substrate, a photoconductive layer positioned over the substrate, electrical contacts in electrical communication with the photoconductive layer, and a dielectric assembly positioned over the photoconductive layer. The dielectric assembly comprises a hydrophobic surface for receiving a liquid. In some embodiments, the dielectric assembly has an effective capacitance of about 10 ?F/m2 to about 10,000 ?F/m2 and/or an average thickness between about 20 nm and about 2000 nm.Type: ApplicationFiled: September 3, 2013Publication date: April 17, 2014Inventors: Srinivas Akella, Vasanthsekar Shekar
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Publication number: 20140102899Abstract: The invention provides a composition including DNA bonded to a plasma-treated surface, the plasma can be any suitable plasma, such as an argon plasma, a compressed air plasma, a flame-based plasma or a vacuum plasma. Surfaces treatable by the methods of the invention include ceramic, metal, fabric and organic polymer surfaces. The DNA can be any DNA, such as a marker DNA, which can be linear or circular, single-stranded or double stranded and from about 25 bases to about 10,000 bases in length. Also provided is a method of binding DNA to a surface, including the steps of exposing the surface to a plasma to produce a plasma-treated surface; and applying DNA to the plasma-treated surface to produce surface bound DNA on the treated surface.Type: ApplicationFiled: March 15, 2013Publication date: April 17, 2014Inventors: Abdelkrim BERRADA, MingHwa Benjamin LIANG, James A. HAYWARD, Lawrence A. JUNG
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Publication number: 20140102901Abstract: The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.Type: ApplicationFiled: September 30, 2013Publication date: April 17, 2014Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Mehdi Javanmard, Sam Emaminejad, Janine Mok, Michael N. Mindrinos
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Patent number: 8697004Abstract: The present teachings provide a device including a memory. According to various embodiments, the memory is readable, writable, and rewritable. The present teachings further provide processing stations, e.g., for carrying out electrophoresis, per, genetic analysis, sample preparation, and/or sample cleanup, etc., that are capable of reading from and/or writing/rewriting to such memory.Type: GrantFiled: May 19, 2010Date of Patent: April 15, 2014Assignee: Applied Biosystems, LLCInventor: Jeffery D. Frazier
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Patent number: 8691067Abstract: A technique for controlling the motion of one or more charged entities linked to a polymer through a nanochannel is provided. A first reservoir and a second reservoir are connected by the nanochannel. An array of electrodes is positioned along the nanochannel, where fluid fills the first reservoir, the second reservoir, and the nanochannel. A first electrode is in the first reservoir and a second electrode is in the second reservoir. The first and second electrodes are configured to direct the one or more charged entities linked to the polymer into the nanochannel. An array of electrodes is configured to trap the one or more charged entities in the nanochannel responsive to being controlled for trapping. The array of electrodes is configured to move the one or more charged entities along the nanochannel responsive to being controlled for moving.Type: GrantFiled: September 16, 2011Date of Patent: April 8, 2014Assignee: International Business Machines CorporationInventors: Stefan Harrer, Binquan Luan, Glenn J. Martyna, Hongbo Peng, Stanislav Polonsky, Stephen M. Rossnagel, Ajay k. Royyuru, Gustavo A. Stolovitzky, George F. Walker
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Publication number: 20140090979Abstract: The combined value of integrating optical forces and electrokinetics allows for the pooled separation vectors of each to be applied, providing for separation based on combinations of features such as size, shape, refractive index, charge, charge distribution, charge mobility, permittivity, and deformability. The interplay of these separation vectors allow for the selective manipulation of analytes with a finer degree of variation. Embodiments include methods of method of separating particles in a microfluidic channel using a device comprising a microfluidic channel, a source of laser light focused by an optic into the microfluidic channel, and a source of electrical field operationally connected to the microfluidic channel via electrodes so that the laser light and the electrical field to act jointly on the particles in the microfluidic channel. Other devices and methods are disclosed.Type: ApplicationFiled: October 1, 2013Publication date: April 3, 2014Inventors: Alexander V. Terray, Sean J. Hart, Sarah J. R. Staton, Gregory E. Collins
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Publication number: 20140087390Abstract: A protocol and system for determining sites at which proteins directly bind to DNA or RNA, modify other proteins including histones, or bind to other proteins as well as determining sites at which DNA or RNA is modified is described herein. A simplified, highly accurate method for studying protein interactions with DNA or RNA and sites of DNA or RNA modification using nanodetector systems is provided.Type: ApplicationFiled: September 25, 2013Publication date: March 27, 2014Applicant: NABsys, Inc.Inventors: John S. Oliver, John Thompson
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Publication number: 20140061048Abstract: The invention relates to devices and methods for nanopore sequencing. The invention provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.Type: ApplicationFiled: September 13, 2013Publication date: March 6, 2014Applicant: Pacific Biosciences of California, Inc.Inventors: Stephen Turner, Benjamin Flusberg
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Patent number: 8663990Abstract: A subject of the present invention is to provide a measurement method using an internal standard substance in an electrophoresis where an analyte is a protein or a compound. The present invention relates to a measurement method for an analyte by an electrophoresis, characterized in that a peak of the analyte is identified by using as an internal standard substance (1) a combination of a compound I having 3 or more anion groups in a molecule and a compound II where 1 to 3 groups of the anion groups of said compound I have been substituted by cation groups, or (2) a combination of a compound III having 3 or more cation groups in a molecule and a compound IV where 1 to 3 groups of the cation groups of said compound III have been substituted by cation groups.Type: GrantFiled: April 27, 2010Date of Patent: March 4, 2014Assignee: Wako Pure Chemical Industries, Ltd.Inventors: Naoyuki Yamamoto, Tatsuo Kurosawa
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Publication number: 20140048416Abstract: A system and method for detecting a single-molecule using an integrated circuit which includes at least one membrane having a nanopore located between first and second reservoirs and a low-noise preamplifier having an electrode formed on the surface thereof is provided. The method includes passing a target molecule through the nanopore, and measuring a current through the nanopore to detect the presence of a biomolecular entity, if any.Type: ApplicationFiled: July 15, 2013Publication date: February 20, 2014Inventors: Jacob Rosenstein, Kenneth L. Shepard
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Patent number: 8652805Abstract: A molecule trapping method includes forming a fluid bridge between a first reservoir and a second reservoir, translocating a molecule from the first reservoir to the second reservoir through the fluid bridge, detecting when a segment of the molecule is in the fluid bridge, breaking the fluid bridge and forming an a gap between the first and the second reservoirs, thereby trapping a segment of the molecule in the gap and making measurements on the segment of the molecule.Type: GrantFiled: August 15, 2011Date of Patent: February 18, 2014Assignee: International Business Machines CorporationInventors: Venkat S. K. Balagurusamy, Stanislav Polonsky
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Publication number: 20140034498Abstract: A device for creating microgradients in solution is disclosed. The device contains a microfluidic channel with openings at each end and two or more small apertures to a bath. Electrodes are placed in the openings at either end of the channel and an electrical power supply is connected to the electrodes. Several distinct current paths exist from one end of the channel to the other. For example current may flow from one electrode, through a portion of the channel, through an aperture into the bath, back through another aperture into the channel, and along another portion of the channel to the other electrode. Current flows along all possible connected paths when an electric field is applied along the channel and induces fluid flow into and out of the apertures in the channel. Fluid flow through the apertures results in the formation of microgradients in solution near the microfluidic channel device.Type: ApplicationFiled: October 17, 2013Publication date: February 6, 2014Applicant: ONDA VIA, INC.Inventors: Mark C. Peterman, David M. Bloom
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Publication number: 20140034496Abstract: The present invention relates to a plastic capillary tube for capillary electrophoresis, in which the plastic capillary tube has an inlet opening and an outlet opening and, furthermore, has at least one hole in the capillary tube wall and the diameter of the hole on the inside of the capillary tube wall dL(innen) lies in the range from 0.5 ?m to 30 ?m.Type: ApplicationFiled: September 23, 2011Publication date: February 6, 2014Applicant: Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V.Inventors: Dominik Mueller, Karsten Pinkwart, Jens Tuebke
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Publication number: 20140038308Abstract: Provided is a process for producing a composite device comprising a light shielding first member and a light transmissive second member, a first surface of the light shielding first member and a second surface of the light transmissive second member being bonded to each other through intermediation of an ultraviolet curing adhesive, the second surface being larger than the first surface, the process including irradiating a region of the second surface to which region the first surface is not bonded with an ultraviolet ray, wherein a reflective member having a reflective surface with an inclination with respect to the second surface onto the light transmissive second member so that the ultraviolet ray that has transmitted through the second surface is reflected toward the ultraviolet curing adhesive between the second surface and the first surface.Type: ApplicationFiled: July 18, 2013Publication date: February 6, 2014Inventor: Yoichi Murakami
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Publication number: 20140034497Abstract: The present disclosure provides devices, systems and methods for sequencing nucleic acid molecules. Nucleic acid molecules can be sequenced with a high accuracy (e.g., greater than 97% in a single pass) using a chip comprising an array of independently addressable nanopore sensors at a density of at least about 500 sites per 1 mm2. An individual nanopore sensor can include a nanopore in a membrane that is adjacent or in proximity to a sensing electrode.Type: ApplicationFiled: June 14, 2013Publication date: February 6, 2014Applicant: Genia Technologies, Inc.Inventors: Randall Davis, Roger Chen, Arkadiusz Bibillo, Kevin Deierling
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Patent number: 8641879Abstract: A technique for controlling the motion of one or more charged entities linked to a polymer through a nanochannel is provided. A first reservoir and a second reservoir are connected by the nanochannel. An array of electrodes is positioned along the nanochannel, where fluid fills the first reservoir, the second reservoir, and the nanochannel. A first electrode is in the first reservoir and a second electrode is in the second reservoir. The first and second electrodes are configured to direct the one or more charged entities linked to the polymer into the nanochannel. An array of electrodes is configured to trap the one or more charged entities in the nanochannel responsive to being controlled for trapping. The array of electrodes is configured to move the one or more charged entities along the nanochannel responsive to being controlled for moving.Type: GrantFiled: September 12, 2012Date of Patent: February 4, 2014Assignee: International Business Machines CorporationInventors: Stefan Harrer, Binquan Luan, Glenn J. Martyna, Hongbo Peng, Stanislav Polonsky, Stephen M. Rossnagel, Ajay K. Royyuru, Gustavo A. Stolovitzky, George F. Walker
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Publication number: 20140027283Abstract: A method for the diagnosis of a benign or malignant bile duct stricture and/or of a CCC, comprising the step of determining at least three polypeptide markers in a body fluid, wherein the polypeptide marker belongs to those markers which in table 1 and/or table 2 a and/or b are characterized by values for the molecular mass and the migration time.Type: ApplicationFiled: December 12, 2011Publication date: January 30, 2014Applicant: MOSAIQUES DIAGNOSTICS AND THERAPEUTICS AGInventor: Harald Mischak
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Publication number: 20140021049Abstract: A flow cell for a microfluidic device can include a chamber, first microfluidic entry and exit channels, second microfluidic entry and exit channels, a first electrode, and a second electrode. A microfluidic device can include a microfluidic channel, a laser to excite fluorescent material, and a detector to detect fluorescence emission. Methods of merging a droplet from an emulsion in to a second stream of fluid and of detecting a content of a droplet in a stream are further disclosed.Type: ApplicationFiled: December 21, 2012Publication date: January 23, 2014Inventors: Tony R. JOAQUIM, Lewis Joe Stafford, Ross Stewart Chambers, Xin Liu, Clive Adrian Smith, Xin Li, Graeme Whyte
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Publication number: 20140021048Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer, such as a base of a DNA or RNA strand.Type: ApplicationFiled: September 27, 2013Publication date: January 23, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jon R. SAUER, Bart J. VAN ZEGHBROECK
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Publication number: 20140021047Abstract: A method and system for analyzing biomolecules using a high concentration electrolytic solution and a low concentration electrolytic solution.Type: ApplicationFiled: February 28, 2013Publication date: January 23, 2014Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventor: Samsung Electronics Co., Ltd.
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Publication number: 20140014513Abstract: Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.Type: ApplicationFiled: May 13, 2013Publication date: January 16, 2014Applicant: Genia Technologies, inc.Inventors: Roger J.A. Chen, Randy Davis
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Publication number: 20140008223Abstract: A method of retrieving sequence-verified deoxyribonucleic acid (DNA) includes positioning a sequence-verified DNA molecule on a microelectromechanical systems (MEMS) substrate. The MEMS substrate includes an electrostatic actuator and the sequence-verified DNA molecule is positioned adjacent to a moving element. The method also includes applying a voltage between the moving element and a stator to cause a motion of the moving element, thereby ejecting the sequence-verified DNA molecule from the MEMS substrate.Type: ApplicationFiled: December 21, 2012Publication date: January 9, 2014Applicant: CAMBRIAN GENOMICS, INC.Inventor: Cambrian Genomics, Inc.
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Publication number: 20140008224Abstract: A detection method of detecting analytes of interest which are present in a liquid. The detection method including the steps of forming drops of liquid on a first surface by capillary breaking of a finger of liquid, which is initially formed by liquid dielectrophoresis. The thus formed drops each come into contact with a different detection surface, which is arranged facing the first surface. Analytes of interest which are present in each of the drops are detected at the corresponding detection surface.Type: ApplicationFiled: February 11, 2011Publication date: January 9, 2014Applicant: Commissariat a l'energie atomique et aux ene altInventors: Vincent Agache, Patrice Caillat, Pierre Puget
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Publication number: 20130344498Abstract: Methods and apparatus providing improved fidelity and specificity when separating nucleic acids from a sample, but without need for amplification. In particular, using the disclosed methods, it is possible to isolate a variant nucleic acid (i.e., a mutation) from a non-target nucleic acid (i.e., a wild-type) when the variant is present in the original sample at a much lower concentration than the non-target, e.g., 1:10,000, without substantial loss of the variant.Type: ApplicationFiled: September 9, 2013Publication date: December 26, 2013Applicant: BOREAL GENOMICS CORP.Inventors: Andrea Marziali, Nitin Sood
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Publication number: 20130337486Abstract: The ability to form and maintain gradients is essential for the study of response of cells to various stimuli. The invention includes devices and methods for the high-throughput, reproducible formation of gradients for the study of living cells. The invention includes microfluidics device with a lest chamber having a depth flanked by flow-through channels having a deeper depth. Flow of two different fluids through the flow-through channels results in the creation of a gradient by diffusion across the test chamber having essentially no flow.Type: ApplicationFiled: May 24, 2013Publication date: December 19, 2013Applicants: UCSD TECH TRANSFER OFFICE, THE JOHNS HOPKINS UNIVERSITYInventors: Andre Levchenko, Alexander Groisman, Saurabh Paliwal
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Publication number: 20130334046Abstract: A method for wetting a nanopore device includes filling a first cavity of the nanopore device with a first buffer solution having a first potential hydrogen (pH) value, filling a second cavity of the nanopore device with a second buffer solution having a second pH value, wherein the nanopore device includes a transistor portion having a first surface, an opposing second surface, and an orifice communicative with the first surface and the second surface, the first surface partially defining the first cavity, the second surface partially defining the second cavity, applying a voltage in the nanopore device, and measuring a current in the nanopore device, the current having a current path partially defined by the first cavity, the second cavity, and the orifice.Type: ApplicationFiled: July 25, 2013Publication date: December 19, 2013Applicant: International Business Machines CorporationInventor: Venkat K. Balagurusamy
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METHOD AND APPARATUS FOR DETECTING LIVER DISEASE, AND METHOD FOR ASSAYING PHARMACEUTICAL PREPARATION
Publication number: 20130330749Abstract: A normal person (i.e. a control) and liver diseases such as drug induced liver injury, an asymptomatic hepatitis B carrier, an asymptomatic hepatitis C carrier, chronic hepatitis B, chronic hepatitis C, liver cancer, a nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and simple steatosis (SS) are identified by measuring the concentrations of ?-Glu-X (X represents an amino acid or an amine) peptides or the levels of AST or ALT in blood and carrying out, for example, a multiple logistic regression based on the measured value.Type: ApplicationFiled: August 15, 2013Publication date: December 12, 2013Inventors: Masahiro SUGIMOTO, Tomoyoshi SOGA -
Publication number: 20130319860Abstract: The invention provides a method for the measurement of a concentration of a charged species in a sample, the sample having a plurality of types of charged species and at least one insoluble component. The method comprises: providing the sample on a surface of a partly permeable layer; allowing components of the sample to pass through the partly permeable layer into a channel; and separating the components into sections, such that each at least one of the sections substantially comprises a single type of the plurality of the types of charged species, and determining the charge concentration in the at least one of the sections.Type: ApplicationFiled: May 16, 2013Publication date: December 5, 2013Applicant: Medimate Holding B.V.Inventors: Steven S. Staal, Jan Floris, Albert Van Den Berg
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Publication number: 20130323185Abstract: The invention provides a method of measuring ammonia and/or calcium in a sample of dental plaque, comprising obtaining the sample of plaque and measuring ammonium and calcium ions using capillary electrophoresis, together with methods of diagnosis, treatment and screening based on evaluation of plaque.Type: ApplicationFiled: February 2, 2011Publication date: December 5, 2013Applicant: COLGATE-PALMOLIVE COMPANYInventors: Stacey Lavender, R. Peter Santarpia, III, Richard J. Sullivan
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Publication number: 20130313113Abstract: Disclosed are systems, devices, methods, and other implementations, including a device to detect at least one target species in a sample, with the device including a microfluidic channel configured to receive the sample containing the at least one target species and a biocompatible ferrofluid in which the at least one target species is suspended, a detector to determine the at least one target species in the sample, and at least two of electrodes positioned proximate the microfluidic channel, the at least two electrodes configured to generate controllable magnetic forces in the sample containing the ferrofluid when a controllable at least one electrical current is applied to the at least two electrodes. The generated controllable magnetic forces causes the at least one target species to be directed towards the detector. Also disclosed are devices for separating target species in a ferrofluid, and for focusing target species suspended in a ferrofluid.Type: ApplicationFiled: June 7, 2011Publication date: November 28, 2013Applicant: YALE UNIVERSITYInventor: Hur Koser
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Publication number: 20130306476Abstract: The invention relates to a nanowire device for manipulation of charged molecules, comprising a tubular nanowire with a through-going channel; a plurality of individually addressable wrap gate electrodes arranged around said tubular nanowire with a spacing between each two adjacent wrap gate electrodes and means for connecting the wrap gate electrodes to a voltage source. The invention further relates to a nanowire system comprising at least one nanowire device, and to a method for manipulating of charged molecules within a through-going channel of a tubular nanowire.Type: ApplicationFiled: February 1, 2012Publication date: November 21, 2013Applicant: QUNANO ABInventors: Lars Samuelson, Jonas Tegenfeldt
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Patent number: 8585973Abstract: In one embodiment, a method is provided for the manufacture of a nano-sensor array. A base having a sensing region is provided along with a plurality of nano-sensors. Each of the plurality of nano-sensors is formed by: forming a first nanoneedle along a surface of the base, forming a dielectric on the first nanoneedle, and forming a second nanoneedle on the dielectric layer. The first nanoneedle of each sensor has a first end adjacent to the sensing region of the base. The second nanoneedle is separated from the first nanoneedle by the dielectric and has a first end adjacent the first end of the first nanoneedle. The base is provided with a fluidic channel. The plurality of nano-sensors and the fluidic channel are configured and arranged with the first ends proximate the fluidic channel to facilitate sensing of targeted matter in the fluidic channel.Type: GrantFiled: May 27, 2011Date of Patent: November 19, 2013Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventor: Hesaam Esfandyarpour
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Publication number: 20130284598Abstract: A method for positioning nano-objects on a surface and an apparatus for implementing the method. The method includes: providing a first surface and a second surface in a position facing each other, where one or more of the surfaces exhibits one or more position structures having dimensions on the nanoscale; providing an ionic liquid suspension of the nano-objects between the two surfaces, where the suspension comprises two electrical double layers each formed at an interface with a respective one of the two surfaces, and the surfaces have electrical charges of the same sign; enabling the nano-objects in the suspension to position according to a potential energy resulting from the electrical charge of the two surfaces; and depositing one or more of the nano-objects on the first surface according to the positioning structures by shifting the minima of the potential energy towards the first surface.Type: ApplicationFiled: April 26, 2013Publication date: October 31, 2013Inventors: Urs T. Duerig, Felix Holzner, Armin W. Knoll, Walter H. Riess
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Publication number: 20130277218Abstract: Devices and methods are provided for performing droplet-based solid phase processing steps on a digital microfluidic device. A solid phase material, which may be a porous solid phase material such as a porous polymer monolith is formed or located on a digital microfluidic element. The solid phase may be formed by an in-situ method in which the digital microfluidic array is actuated to transport a droplet of solid phase pre-cursor solution to a selected element on the array, and subsequently processed to form a solid phase on the array element. The integration of a solid phase material with a digital microfluidic array enables a wide range of applications including solid phase extraction and sample concentration.Type: ApplicationFiled: October 3, 2011Publication date: October 24, 2013Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTOInventors: Jared M. Mudrik, Hao Yang, Aaron R. Wheeler
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Patent number: 8564760Abstract: A capillary passage is formed between a substrate holder and an edge structure. Along the capillary passage are arranged a plurality of electrodes which, when charged, become liquidphilic. The electrodes may be used to split droplets of liquid and pump the liquid along the capillary passage.Type: GrantFiled: October 14, 2010Date of Patent: October 22, 2013Assignee: ASML Netherlands B.V.Inventors: Martinus Hendrikus Antonius Leenders, Sjoerd Nicolaas Lambertus Donders, Nicolaas Rudolf Kemper
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Publication number: 20130270114Abstract: Digital microfluidics system manipulates samples in liquid droplets within disposable cartridges that have bottom layer, top layer, and gap between the bottom and top layers. The system has a base unit with cartridge accommodation sites and a central control unit for controlling selection of individual electrodes of electrode arrays and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the cartridges by electrowetting. The system further has board accommodation sites located at the cartridge accommodation sites that each can take up a swappable electrode board having an electrode array and electrical board contact elements individually connected to electrodes of the electrode array. Each board accommodation site has electrical base unit contact elements that are connected to the central control unit and that are configured to engage with the electrical board contact elements of a swappable electrode board that is placed at the board accommodation site.Type: ApplicationFiled: May 23, 2013Publication date: October 17, 2013Inventor: Marc N. FEIGLIN
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Publication number: 20130264205Abstract: An efficient method of processing a target material in a sample using a microfluidic device including an elastic membrane.Type: ApplicationFiled: December 21, 2012Publication date: October 10, 2013Applicant: SAMSUNG ELECTRONICS CO., LTD.Inventors: Kyu-youn HWANG, Sung-hong KWON, Joon-ho KIM
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Publication number: 20130264204Abstract: A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). The semiconductor device includes at least two doped regions, such as two n-typed regions implanted in a p-typed semiconductor layer or two p-typed regions implanted in an n-typed semiconductor layer. The detecting region permits a current to pass between the two doped regions in response to the presence of the component of the polymer.Type: ApplicationFiled: September 14, 2012Publication date: October 10, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jon R. Sauer, Bart J. van Zeghbroeck
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Publication number: 20130260472Abstract: Described are devices and methods for forming one or more nanomembranes including electroactive nanomembranes within a nanowell or nanotube, or combinations thereof, in a support material. Nanopores/nanochannels can be formed by the electroactive nanomembrane within corresponding nanowells. The electroactive nanomembrane is capable of controllably altering a dimension, a composition, and/or a variety of properties in response to electrical stimuli. Various embodiments also include devices/systems and methods for using the nanomembrane-containing devices for molecular separation, purification, sensing, etc.Type: ApplicationFiled: April 2, 2012Publication date: October 3, 2013Applicant: Lux Bio Group, Inc.Inventor: Gordon HOLT
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Publication number: 20130260371Abstract: Described are devices and methods for forming one or more nanomembranes including electroactive nanomembranes within a nanowell or nanotube, or combinations thereof, in a support material. Nanopores/nanochannels can be formed by the electroactive nanomembrane within corresponding nanowells. The electroactive nanomembrane is capable of controllably altering a dimension, a composition, and/or a variety of properties in response to electrical stimuli. Various embodiments also include devices/systems and methods for using the nanomembrane-containing devices for molecular separation, purification, sensing, etc.Type: ApplicationFiled: April 2, 2012Publication date: October 3, 2013Applicant: LUX BIO GROUP, INC.Inventor: Gordon HOLT
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Patent number: 8545686Abstract: The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.Type: GrantFiled: April 5, 2006Date of Patent: October 1, 2013Assignee: Hitachi High-Technologies CorporationInventors: Ryoji Inaba, Tomoyuki Sakai, Motohiro Yamazaki, Takashi Gomi, Kazumichi Imai
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Publication number: 20130240356Abstract: Methods, devices, substrates, and systems are disclosed involving arrays of zero-mode waveguides having nanopores extending through the bases that form the bottoms of the zero-mode-waveguides. Electric fields across the nanopores are used to attach single biomolecules such as polymerase enzymes within each zero-mode-waveguide. Electric fields across the nanopores can also be used for the active loading of nucleic acid templates into enzymes attached within the zero mode waveguides.Type: ApplicationFiled: November 5, 2012Publication date: September 19, 2013Applicant: Pacific Biosciences of California, Inc.Inventors: Meni Wanunu, Jonas Korlach, Mathieu Foquet, Stephen Turner
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Publication number: 20130240359Abstract: The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid.Type: ApplicationFiled: May 14, 2013Publication date: September 19, 2013Applicant: Pacific Biosciences of California, Inc.Inventors: Stephen Turner, Jeffrey Wegener