Lysine; Diaminopimelic Acid; Threonine; Valine Patents (Class 435/115)
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Patent number: 7432085Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: November 18, 2005Date of Patent: October 7, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Publication number: 20080241862Abstract: While the identification of acetylated lysine residues on proteins is well-known, the modification of lysine residues through propionylation and butyrylation is not very well understood. A method for the identification and mapping of propionylated and butyrylated lysine residues has been developed. Anti-acetyllysine antibody, normally used to affinity purify a protein mixture based on the presence of acetylated lysine, can also be used to affinity purity proteins having propionylated and butyrylated lysine residues due to the structural similarity. The method involves searching protein databases to locate mass spectrometry datasets for those proteins purified by anti-acetyllysine antibody. The located spectra are manually reviewed to identify those peptides having propionyllysine and butyryllysine residues. These identified peptides are synthesized, with the lysine modifications added at the appropriate positions.Type: ApplicationFiled: January 29, 2008Publication date: October 2, 2008Inventors: YINGMING ZHAO, John Falck, Yue Chen
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Patent number: 7416740Abstract: Hydrochloric acid, sulfuric acid or an L-lysine solution having an equivalent ratio of anion/L-lysine higher than 0.95 is added to a raw material L-lysine solution having an equivalent ratio of anion/L-lysine lower than 0.68 to adjust the equivalent ratio of anion/L-lysine of the raw material solution to be in the range of 0.68 to 0.95, and the obtained L-lysine solution or a concentrate thereof is granulated and dried to obtain a dry granulated product having a high L-lysine content and showing low caking property and low hygroscopic property.Type: GrantFiled: January 24, 2003Date of Patent: August 26, 2008Assignee: Ajinomoto Co., Inc.Inventors: Takeshi Kushiki, Junko Morikawa, Kazuhiko Hasegawa
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Publication number: 20080199919Abstract: A DNA encoding a variant of a protein having a loop region and six hydrophobic helixes which is involved in excretion of L-lysine to outside of a cell is described, wherein the DNA encodes a mutant protein which does not contain the loop region that is present in the wild-type protein. The mutant protein facilitates excretion of L-lysine, L-arginine, or both to the outside of the cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium. Specifically, lysE24 is introduced into a methanol assimilating bacterium such as Methylophilus bacteria which results in improved L-amino acid productivity, especially production of L-lysine and L-arginine.Type: ApplicationFiled: November 27, 2006Publication date: August 21, 2008Inventors: Yoshiya Gunji, Hisashi Yasueda
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Publication number: 20080199920Abstract: Provided is S-adenosylmethionine-6-N-lysine-methyl-transferase obtained from Ne?rospora crassa, a polynucleotide encoding the same, a vector and host cell containing the polynucleotide, and a method of producing trimethyllysine by culturing the host cell.Type: ApplicationFiled: July 7, 2006Publication date: August 21, 2008Applicant: CJ CHEILJEDANG CORP.Inventors: Whan-Koo Kang, Bheong-Uk Lee, Young-Hoon Park, Eun-Sung Koh, Jae-Yeong Ju, Jin-Ho Lee, Hye-Won Kim, Hye-Jin Choi
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Publication number: 20080193985Abstract: The invention relates to a method for recovering a basic amino acid from the fermentation liquor of a micro-organism strain that produces the basic amino acid. According to said method: a) the fermentation liquor is acidified with an acid, whose pKs value in water at 25° C. ranges between 2 and 5; and b) the basic amino acid is separated from the aqueous liquor obtained in step a) by the successive charging of a single or multiple stage, serial arrangement of a strongly acidic cation exchanger in the form of a salt with the liquor that has been obtained in step a) and the subsequent elution of the basic amino acid using a basic eluant.Type: ApplicationFiled: April 13, 2006Publication date: August 14, 2008Applicant: BASF Aktiengesellschaft Patents, Trademarks and LicensesInventors: Jong-Soo Choi, Tae-Hui Kim, Sung Hyun Kim
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Publication number: 20080176296Abstract: The present invention relates to isolated nucleic acid molecules encoding mutants of glucose-6-phosphate dehydrogenase, and vectors and hosts cells including such nucleic acid molecules. These nucleic acid molecules are involved in the biosynthesis of a fine chemical, e.g., an amino acid such as lysine. The present invention also relates to methods of producing and modulating the production of fine chemicals, e.g., lysine, by culturing recombinant microorganisms containing these nucleic acid molecules under conditions such that the fine chemical is produced.Type: ApplicationFiled: March 26, 2008Publication date: July 24, 2008Inventors: Oskar Zelder, Markus Pompejus, Hartwig Schroder, Burkhard Kroger, Corinna Klopprogge, Gregor Haberhauer
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Publication number: 20080176295Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: March 17, 2008Publication date: July 24, 2008Inventors: Oskar Zelder, Corinna Klopprogge, Burkhard Kroger, Hartwig Schroder, Stefan Haefner
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Patent number: 7399617Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of an L-amino acid excretion protein is described. A method for producing the L-amino acid using the bacterium is also described.Type: GrantFiled: March 3, 2006Date of Patent: July 15, 2008Assignee: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadievich Livshits, Natalia Pavlovna Zakataeva, Kazuo Nakanishi, Vladimir Veniaminovich Aleshin, Petr Vladimirovich Troshin, Irina Lyvovna Tokhmakova
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Publication number: 20080166775Abstract: The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.Type: ApplicationFiled: February 27, 2008Publication date: July 10, 2008Applicant: BASF AktiengesellschaftInventors: Burkhard Kroger, Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner
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Publication number: 20080166774Abstract: The invention relates to a method for producing an amino acid comprising culturing a microorganism of the genus Corynebacterium or Brevibacterium wherein said microorganism is partially or completely deficient in at least one of the gene loci of the group which is formed by otsAB, treZ and treS, and subsequent isolation of the amino acid from the culture medium.Type: ApplicationFiled: January 28, 2008Publication date: July 10, 2008Applicant: BASF AGInventors: Corinna Klopprogge, Oskar Zelder, Burkhard Kroger, Hartwig Schroder, Stefan Hafner, Wolfgang Liebl
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Publication number: 20080160585Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.Type: ApplicationFiled: January 28, 2008Publication date: July 3, 2008Applicant: BASF AktiengesellschaftInventors: Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Hafner, Burkhard Kroger, Patrick Kiefer, Elmar Heinzle, Christoph Wittmann
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Publication number: 20080153139Abstract: The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacteriuim glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.Type: ApplicationFiled: April 6, 2006Publication date: June 26, 2008Inventors: Jan Marienhagen, Lothar Eggeling, Hermann Sahm
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Publication number: 20080131942Abstract: The invention relates to a process for producing corynebacteria comprising one or more modified genomic sequences, where a vector is used which does not replicate in corynebacteria and whose nucleic acid is not recognized by corynebacteria as foreign.Type: ApplicationFiled: February 11, 2005Publication date: June 5, 2008Applicant: BASF AktiengesellschaftInventors: Markus Pompejus, Hartwig Schroder, Burkhard Kroger, Oskar Zelder
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Publication number: 20080131903Abstract: The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.Type: ApplicationFiled: January 18, 2008Publication date: June 5, 2008Applicant: EVONIK DEGUSSA GmbhInventors: Georg THIERBACH, Brigitte Bathe, Natalie Schischka
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Patent number: 7381548Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the yafA gene.Type: GrantFiled: December 12, 2005Date of Patent: June 3, 2008Assignee: Ajinomoto Co., Inc.Inventors: Marina Evgenievna Sheremet'eva, Konstantin Vyacheslavovich Rybak, Tatyana Viktorovna Leonova, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov
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Publication number: 20080118472Abstract: Methods for increasing the resistance to rumen inactivation of a cultured Gram positive bacteria strain useful for gastrointestinal delivery of bioactive compounds to ruminants, which includes the steps of growing a culture of the bacteria strain through at least one passage in a growth medium containing an amount of lysozyme effective to induce the growth of bacterial cell walls resistant to protozoal predation; and recovering the bacteria strain from the lysozyme-containing medium. Rumen-bypass feed supplements produced by the inventive method are also disclosed, as well as methods for supplementing the diet of a ruminant with the rumen bypass feed supplements and an in vitro method for evaluating the resistance of Gram positive bacteria strains to rumen inactivation in vivo.Type: ApplicationFiled: December 6, 2005Publication date: May 22, 2008Applicant: Sage Biosciences Inc.Inventors: Lyle M. Rode, C. James Newbold
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Patent number: 7368266Abstract: A method for producing L-threonine using a microorganism is provided. In the method, additional one or more copies of each of the phosphoenolpyruvate carboxylase (ppc) gene and the threonine operon are integrated into a particular site of the chromosomal DNA of a microorganism, while its inherent ppc gene and threonine operon remain. Accordingly, two or more ppc genes and threonine operons are included in the chromosomal DNA of the microorganism to thereby enhance the expression of the ppc gene encoding an enzyme to convert phosphoenolpyruvate to a threonine biosynthesis precursor, oxaloacetete, and the genes encoding enzymes involved in the synthetic pathway of threonine from oxaloacetate, including thrA (aspartokinasel-homoserine dehydrogenase), thrB (homoserine kinase), and thrC (threonine synthase), thereby markedly increasing L-threonine productivity.Type: GrantFiled: February 1, 2005Date of Patent: May 6, 2008Assignee: CJ CorporationInventors: Kap-Soo Noh, Yeong-Chul Kim, Jae-Yong Park, Dai-Chul Kim, Jin-Ho Lee, Seung-Han Ok
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Patent number: 7354745Abstract: Purified ?-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure ?-amino acids, such as L-?-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-?-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-?-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-?-lysine in vitro.Type: GrantFiled: May 1, 2001Date of Patent: April 8, 2008Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 7335506Abstract: A DNA encoding a variant of a protein, the protein having a loop region and six hydrophobic helices and involved in secretion of L-lysine to the outside of a cell, wherein the DNA encodes a variant of a protein not containing the loop region and facilitates secretion of L-lysine, L-arginine or both of these L-amino acids to the outside of a methanol-assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a Methylobacillus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.Type: GrantFiled: November 20, 2003Date of Patent: February 26, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoshiya Gunji, Hisashi Yasueda
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Patent number: 7332309Abstract: The invention relates to a process for the preparation of L-amino acids, in particular L-threonine, in which the following steps are carried out: a) fermentation of microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid and in which at least one or more of the genes chosen from the group consisting of sucC and sucD, or nucleotide sequences which code for these, is or are enhanced, in particular over-expressed, b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the desired L-amino acid.Type: GrantFiled: July 3, 2002Date of Patent: February 19, 2008Assignee: Degussa AGInventor: Mechthild Rieping
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Patent number: 7332310Abstract: Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.Type: GrantFiled: March 22, 2004Date of Patent: February 19, 2008Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Nakagawa, Hiroshi Mizoguchi, Seiko Ando, Mikiro Hayashi, Keiko Ochiai, Haruhiko Yokoi, Naoko Tateishi, Akihiro Senoh, Masato Ikeda, Akio Ozaki
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Publication number: 20080038787Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.Type: ApplicationFiled: December 17, 2004Publication date: February 14, 2008Applicant: Basf AktiengesellschaftInventors: Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Hafner, Burkhard Kroger, Patrick Kiefer, Elmar Heinzle, Christoph Wittmann
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Publication number: 20080026432Abstract: The present invention relates to a preparation method of an L-threonine producing strain by utilizing a novel L-threonine importer identified from Corynebacterium glutamicum. The method can be advantageously used for the production of L-threonine by increasing the fermentation concentration of Lthreonine and the yield per unit thereof.Type: ApplicationFiled: November 23, 2004Publication date: January 31, 2008Inventors: Young-Hoon Park, Sang-Jo Lim, Seong-Jun Kim
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Patent number: 7323321Abstract: The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be quantified by the formula: [(g amino acid produced/g dextrose consumed)*100]=% Yield and expressed as yield from dextrose. The invention provides microorganisms that are made bradytrophic for the synthesis of valine by mutagenesis.Type: GrantFiled: December 28, 2005Date of Patent: January 29, 2008Assignee: Archer-Daniels-Midland CompanyInventors: P. John Rayapati, Corey M. Crafton
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Patent number: 7319026Abstract: The invention relates to amino acid-producing bacteria from the family Enterobacteriaceae, in particular from the species Escherichia coli, which contain a stop codon chosen from the group amber, ochre and opal in the nucleotide sequence for the coding region of the rpoS gene and a suppressor for a stop codon chosen from the group amber suppressor, ochre suppressor and opal suppressor. This invention also relates to a process for preparing amino acids, in particular L-threonine, using these bacteria.Type: GrantFiled: January 21, 2003Date of Patent: January 15, 2008Assignee: Degussa AGInventors: Mechthild Rieping, Nicole Siebelt
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Patent number: 7306932Abstract: The invention relates to alleles of the glk gene from coryneform bacteria coding for glucokinases, and to processes for the production of L-lysine by fermentation using bacteria containing such alleles.Type: GrantFiled: November 7, 2002Date of Patent: December 11, 2007Assignee: Degussa AGInventors: Brigitte Bathe, Stephan Hans, Caroline Reynen
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Patent number: 7306933Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.Type: GrantFiled: January 3, 2006Date of Patent: December 11, 2007Assignee: Ajinomoto Co., Inc.Inventors: Stephen Van Dien, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui, Yuta Nakai, Tomoko Suzuki, Mika Moriya, Yuichiro Tsuji, Takuji Ueda
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Patent number: 7300777Abstract: The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.Type: GrantFiled: May 25, 2005Date of Patent: November 27, 2007Assignees: Archer-Daniels-Midland Company, Massachusetts Institute of TechnologyInventors: Paul D. Hanke, Anthony J. Sinskey, Laura B. Willis, Stephane Guillouet
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Patent number: 7288393Abstract: A nucleotide sequence of the threonine operon of E. coli with a deletion of all or part of a nucleotide fragment of ?56 to ?18, a recombinant vector containing the above nucleotide sequence, and a transformed host cell containing the recombinant vector are provided. A method for producing L-threonine comprising culturing the transformed host cell is also provided.Type: GrantFiled: May 15, 2003Date of Patent: October 30, 2007Assignee: CJ CorporationInventors: Hee Jong Lee, Seong Jun Kim, Jin Suck Sung, Seok Won Song, Young Hoon Park
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Patent number: 7267967Abstract: A pyruvate carboxylase gene having SEQ ID NO:1 as well as an amino acid sequence having SEQ ID NO:2 expressed by the pyruvate carboxylate gene are disclosed. Also disclosed is a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate carboxylase activity is increased by genetically changing the pyruvate carboxylase gene having SEQ ID NO:1 through expression of a microorganism which produces the corresponding amino acid having SEQ ID NO:2.Type: GrantFiled: September 30, 1998Date of Patent: September 11, 2007Assignee: Forschungszentrum Julich GmbHInventors: Bernd Eikmanns, Petra Peters-Wendisch, Hermann Sahm
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Patent number: 7262036Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.Type: GrantFiled: May 22, 2006Date of Patent: August 28, 2007Assignee: Degussa AGInventors: Bettina Hädrich, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
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Patent number: 7259003Abstract: A method for producing an L-amino acid, such as L-phenylalanine or L-threonine, is provided using a bacterium belonging to the genus Escherichia, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of the protein encoded by the yedA gene.Type: GrantFiled: November 25, 2002Date of Patent: August 21, 2007Assignee: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadyevich Livshits, Maria Viacheslavovna Vitushkina, Mikhail Markovich Gusyatiner, Mikhail Kharisovich Ziyatdinov, Valery Zavenovich Akhverdian, Ekaterina Alekseevna Savrasova, Vera Georgievna Doroshenko, Sergey Vladimirovich Mashko
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Patent number: 7256021Abstract: The invention relates to a process for the preparation of L-amino acids, especially L-lysine, L-valine, L-homoserine and L-threonine, by fermenting a microorganism of the genus Escherichia which has a mutation or deletion in the gene encoding aspartate ammonium lyase (aspA).Type: GrantFiled: July 3, 2002Date of Patent: August 14, 2007Assignee: Degussa AGInventor: Thomas Hermann
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Patent number: 7252977Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sugar import protein K (msiK) gene, and a host-vector system having a coryneform host bacterium in which the msiK gene is present in enhanced form and a vector which carries at least the msiK gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: September 26, 2001Date of Patent: August 7, 2007Assignee: Degussa AGInventors: Brigitte Bathe, Natalie Schischka, Mike Farwick, Walter Pfefferle
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Patent number: 7241600Abstract: The invention relates to a process for the preparation of L-amino acids, in particular L-threonine.Type: GrantFiled: June 14, 2002Date of Patent: July 10, 2007Assignee: Degussa AGInventor: Mechthild Rieping
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Patent number: 7229802Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.Type: GrantFiled: September 2, 2003Date of Patent: June 12, 2007Assignee: Degussa AGInventors: Brigitte Bathe, Natalie Schischka, Michael Bott, Steffen Schaffer
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Patent number: 7226763Abstract: An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknD gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.Type: GrantFiled: April 21, 2005Date of Patent: June 5, 2007Assignee: Degussa AGInventors: Brigitte Bathe, Indra Schroeder, Mike Farwick, Thomas Hermann
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Patent number: 7226762Abstract: The present invention relates to isolated nucleic acid molecules encoding mutants of glucose-6-phosphate dehydrogenase, and vectors and hosts cells including such nucleic acid molecules. These nucleic acid molecules are involved in the biosynthesis of a fine chemical, e.g., an amino acid such as lysine. The present invention also relates to methods of producing and modulating the production of fine chemicals, e.g., lysine, by culturing recombinant microorganisms containing these nucleic acid molecules under conditions such that the fine chemical is produced.Type: GrantFiled: November 11, 2002Date of Patent: June 5, 2007Assignee: BASF AktiengesellschaftInventors: Oskar Zelder, Markus Pompejus, Hartwig Schröder, Burkhard Kröger, Corinna Klopprogge, Gregor Haberhauer
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Patent number: 7223572Abstract: An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or a Methylophilus bacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.Type: GrantFiled: April 7, 2000Date of Patent: May 29, 2007Assignee: Ajinomoto Co., Inc.Inventors: Yoshiya Gunji, Hisashi Yasueda, Shinichi Sugimoto, Nobuharu Tsujimoto, Megumi Shimaoka, Yuri Miyata, Manami Oba
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Patent number: 7220572Abstract: There is provided a method for producing L-leucine using bacterium belonging to the genus Escherichia, which produces L-valine, L-isoleucine and L-homoleucine in an amount of less than 1% of that of L-leucine produced due to inactivation of ilvE gene coding for branched chain amino acid transaminase and produces increased amount of L-leucine due to increasing the activity of the aromatic amino acid transaminase encoded by tyrB gene.Type: GrantFiled: June 24, 2003Date of Patent: May 22, 2007Assignee: Ajinomoto Co., Inc.Inventors: Mikhail Markovich Cusyatiner, Elvira Borisovna Voroshilova, Yulia Georgievna Rostova, Lirina Valerievna Ivanovskaya, Maria Grigorievna Lunts, Evgeniy Moiseevich Khourges
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Patent number: 7211421Abstract: An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase (named dapB) is obtained from the transformant.Type: GrantFiled: March 8, 2005Date of Patent: May 1, 2007Assignee: Ajinomoto Co., Inc.Inventors: Nobuharu Tsujimoto, Hisashi Yasueda, Yoshio Kawahara, Shinichi Sugimoto
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Patent number: 7211415Abstract: The present invention relates to a process for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, wherein a) the yfiD ORF and/or the pflB gene or nucleotide sequences coding for the gene products are overexpressed in the microorganisms producing the desired L-amino acid, and the microorganisms are cultured in a medium under conditions in which the desired L-amino acid is enriched in the medium or in the cells; and b) the desired L-amino acid is isolated, in a manner such that constituents of the fermentation broth and/or the biomass in its entirety or in portions (>0 to 100%) either remain in the isolated product or are completely removed.Type: GrantFiled: April 5, 2004Date of Patent: May 1, 2007Assignee: Degussa AGInventors: Mechthild Rieping, Mike Farwick
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Patent number: 7211416Abstract: L-Lysine is produced by culturing a methanol-utilizing bacterium which requires L-methionine for its growth and has an ability to produce L-lysine in a medium containing methanol as a main carbon source to produce and accumulate L-lysine in culture and collecting the L-lysine from the culture.Type: GrantFiled: January 21, 2004Date of Patent: May 1, 2007Assignee: Ajinomoto Co., Inc.Inventors: Takayuki Asahara, Seiko Hirano, Hisashi Yasueda
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Patent number: 7202061Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the mikE17 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: August 24, 2004Date of Patent: April 10, 2007Assignee: Degussa GmbHInventors: Mike Farwick, Klaus Hutchmacher, Walter Pfefferle
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Patent number: 7192749Abstract: Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene of C. glutamicum and which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids.Type: GrantFiled: May 13, 2003Date of Patent: March 20, 2007Assignee: BASF AktiengesellschaftInventors: Corinna Klopprogge, Oskar Zelder, Burkhard Kröger, Hartwig Schröder, Stefan Häfner, Heung-Shick Lee
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Patent number: 7186531Abstract: There is disclosed a method for producing L-threonine using bacterium belonging to the genus Escherichia wherein the bacterium has been modified to enhance an activity of aspartate-?-semialdehyde dehydrogenase.Type: GrantFiled: December 3, 2004Date of Patent: March 6, 2007Assignee: Ajinomoto Co., Inc.Inventors: Valery Zavenovich Akhverdian, Ekaterina Alekseevna Savrasova, Alla Markovna Kaplan, Andrey Olegovich Lobanov, Yuri Ivanovich Kozlov
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Patent number: 7179623Abstract: Amino acids such as threonine, homoserine, isoleucine, lysine, valine and tryptophan are produced using a bacterium belonging to the genus Escherichia which has been constructed from a sucrose non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS or PTS genes and has an ability to produce the amino acid.Type: GrantFiled: August 16, 2005Date of Patent: February 20, 2007Assignee: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadyevich Livshits, Vera Georgievna Doroshenko, Sergei Vladimirovich Mashko, Valery Zavenovich Akhverdian, Yury Ivanovich Kozlov
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Patent number: 7173105Abstract: The present invention relates to polynucleotides corresponding to the luxR gene and which encode a LuxR transcriptional activator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LuxR transcriptional activation activity.Type: GrantFiled: December 27, 2004Date of Patent: February 6, 2007Assignee: Degussa AGInventors: Bettina Moeckel, Caroline Kreutzer, Brigitte Bathe
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Patent number: 7169587Abstract: A DNA encoding a variant of a protein, having a loop region and six hydrophobic helixes and involved in excretion of L-lysine to outside of a cell, wherein the DNA encodes a mutant protein not containing the loop region that is contained in a wild-type protein and facilitates excretion of L-lysine, L-arginine or both of these L-amino acids to outside of a cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a methanol assimilating bacterium such as Methylophilus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.Type: GrantFiled: June 11, 2002Date of Patent: January 30, 2007Assignee: Ajinomoto Co., Inc.Inventors: Yoshiya Gunji, Hisashi Yasueda