Lysine; Diaminopimelic Acid; Threonine; Valine Patents (Class 435/115)
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Patent number: 7163810Abstract: In a method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, there are used, as the microorganism, a microorganism to which a methanol dehydrogenase gene is introduced, of which activities of hexulose phosphate synthase and phosphohexuloisomerase are enhanced and which is modified so that an ability to utilize methanol should be imparted or enhanced, and there is used a medium containing methanol as a carbon source.Type: GrantFiled: September 5, 2002Date of Patent: January 16, 2007Assignee: Ajinomoto Co., Inc.Inventors: Hisashi Yasueda, Ryo Takeshita
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Patent number: 7160703Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.Type: GrantFiled: June 13, 2003Date of Patent: January 9, 2007Assignee: Degussa AGInventors: Bettina Moeckel, Stephan Hans, Natalie Schischka, Walter Pfefferle
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Patent number: 7160704Abstract: The present invention discloses a method for producing a target substance using a coryneform bacterium comprising culturing a coryneform bacterium having an ability to produce the target substance in a medium, resulting in accumulation of the target substance in the medium or cells of the bacterium, and collecting the target substance from the medium or the cells of the bacterium. Also disclosed is a coryneform bacterium which is introduced with a methanol dehydrogenase gene and which has enhanced activities of hexulose phosphate synthase and phosphohexuloisomerase, and to which an ability to utilize methanol is imparted or which has enhanced ability to utilize methanol, and the medium contains methanol as a carbon source.Type: GrantFiled: March 4, 2004Date of Patent: January 9, 2007Assignee: Ajinomoto Co., Inc.Inventors: Ryo Takeshita, Hisashi Yasueda
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Patent number: 7150985Abstract: The inventions relate to the field of medicine, veterinary and biotechnology and may be used for production of a drug for medical and veterinary purposes. A bacteriolytic complex, produced by the bacterium Lysobacter sp. XL1, has been proposed, which contains bacteriolytic enzymes (muramidase, muramoylalanineamidase, endopeptidase, a bacteriolytic enzyme with a molecular weight of about 22 kDa), protease, polysaccharide, and ballast components. The method of production of the bacteriolytic complex includes cultivation of the strain-producer on a nutrient medium containing glucose, peptone, yeast extract or yeast autolysate, phosphate salts of sodium and potassium, magnesium sulfate, potassium chloride, iron sulfate, and water.Type: GrantFiled: November 28, 2001Date of Patent: December 19, 2006Assignee: Institut Biokhimii I Fisiologii Mikroorganismov IM. G.K.Skrjabina Rossiiskoi Akademii Nauk (IBFM RAN)Inventors: Igor Stepanovich Kulaev, Olga Andreevna Stepnaja, Irina Matveevna Zfasman, Taisija Sergeevna Tchermenskaja, Larisa Aleksandrovna Ledova, Ljudmila Grigorevna Zubrizkaja, Vassily Konstantinovich Akimenko
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Patent number: 7138266Abstract: A bacterial strain of Escherichia coli is described which produces L-threonine, and is obtained by a process comprising transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity, and isolation of a transductant lacking threonine dehydrogenase activity.Type: GrantFiled: September 2, 2003Date of Patent: November 21, 2006Assignee: Ajinomoto Co., Inc.Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
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Patent number: 7135313Abstract: Alleles of the lysC gene from corynebacteria that code for desensitized aspartokinases, and to processes for the preparation of L-lysine using bacteria containing these alleles.Type: GrantFiled: June 14, 2004Date of Patent: November 14, 2006Assignee: Degussa AGInventors: Brigitte Bathe, Stephen Hans
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Patent number: 7129066Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.Type: GrantFiled: February 28, 2003Date of Patent: October 31, 2006Assignee: Degussa AGInventors: Mike Farwick, Klaus Huthmacher, Achim Marx, Brigitte Bathe, Walter Pfefferle
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Patent number: 7122369Abstract: The invention provides novel microorganisms, methods for the production thereof and novel processes for the production of amino acids. Mutagenesis of parental bacterial strains and selection of an improved raffinate-resistant phenotype enables the isolation of strains with enhanced growth properties that produce larger amounts of amino acid. Microorganisms of the invention are produced from amino acid producing parental strains such as Corynebacterium or Brevibacterium, particularly preferred are parental strains that produce L-lysine.Type: GrantFiled: October 30, 2002Date of Patent: October 17, 2006Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J Liaw, John Eddington, Yueqin Yang, Richard Dancey, Stacia Swisher, Weiying Mao
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Patent number: 7118894Abstract: The invention provides novel microorganisms, methods for the production thereof and novel processes for the production of amino acids. Mutagenesis of parental bacterial strains and selection of an improved raffinate-resistant phenotype enables the isolation of strains with enhanced growth properties that produce larger amounts of amino acid. Microorganisms of the invention are produced from amino acid producing parental strains such as Corynebacterium or Brevibacterium, particularly preferred are parental strains that produce L-lysine.Type: GrantFiled: October 30, 2002Date of Patent: October 10, 2006Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J Liaw, John Eddington, Yueqin Yang, Richard Dancey, Stacia Swisher, Weiying Mao
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Patent number: 7105321Abstract: The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.Type: GrantFiled: December 2, 2003Date of Patent: September 12, 2006Assignee: Degussa AGInventors: Bettina Moeckel, Caroline Kreutzer, Thomas Hermann, Mike Farwick, Achim Marx, Walter Pfefferle
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Patent number: 7097999Abstract: The present invention describes a method for producing a target substance by utilizing a microorganism comprising culturing the microorganism in a medium, allowing the target substance to accumulate, and collecting the target substance from the medium. Also the microorganism used in the present invention is a mutant strain whereby maltose assimilation is controlled by the interaction between IIAGlc protein of glucose PTS and MalK.Type: GrantFiled: April 15, 2002Date of Patent: August 29, 2006Assignee: Ajinomoto Co., Inc.Inventors: Nobuharu Tsujimoto, Tomoko Suzuki, Hisao Ito
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Patent number: 7094584Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.Type: GrantFiled: March 19, 2004Date of Patent: August 22, 2006Assignee: Degussa AGInventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Möckel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
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Patent number: 7078204Abstract: The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant. According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.Type: GrantFiled: June 15, 2001Date of Patent: July 18, 2006Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Haruhiko Yokoi, Seiko Ando, Keiko Ochiai, Yoshiyuki Yonetani, Shin-ichi Hashimoto
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Patent number: 7074602Abstract: An L-threonine-producing Escherichia coli strain and a method for producing the same are provided. The Escherchia coli strain contains chromosomal DNA with inactivated metJ gene. Therefore, expression repression of threonine biosynthesis genes by a metJ gene product is prevented, thereby producing a high concentration of threonine. Further, a high concentration of L-threonine can be produced in high yield using the method.Type: GrantFiled: August 12, 2004Date of Patent: July 11, 2006Assignee: CJ CorporationInventors: Jae Yong Cho, Byoung Choon Lee, Dae Cheol Kim, Jin Ho Lee, Young Hoon Park
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Patent number: 7074607Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.Type: GrantFiled: August 14, 2003Date of Patent: July 11, 2006Assignee: Degussa AGInventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
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Patent number: 7067288Abstract: The invention provides nucleotide sequences from coryneform bacteria which code for maltate dehydrogenase and a process for the fermentative preparation of amino acids using bacteria in which the mdhA gene is attenuated.Type: GrantFiled: December 5, 2003Date of Patent: June 27, 2006Assignee: Degussa AGInventors: Douwe Molenaar, Michel Eduard Van Der Rest, Andre Drysch
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Patent number: 7052883Abstract: The invention relates to a process for producing an L-amino acid, in particular, L-threonine, which comprises: cultivating a microorganism of the family Enterobacteriaceae producing the desired L-amino acid under conditions suitable for production of the amino acid, in which the fruR (cra) (fructose repressor) gene or nucleotide secluences coding therefor in the microorganism is attenuated, in particular are switched off or eliminated, and recovering the L-amino acid from the medium or in the cells of the microorganism.Type: GrantFiled: April 3, 2002Date of Patent: May 30, 2006Assignee: Degussa AGInventors: Mechthild Rieping, Thomas Hermann
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Patent number: 7049106Abstract: A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.Type: GrantFiled: April 9, 2002Date of Patent: May 23, 2006Assignee: Degussa AGInventors: Mike Farwick, Brigitte Bathe, Thomas Hermann, Achim Marx, Walter Pfefferle
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Patent number: 7037689Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigC gene from Corynebacterium glutamicum, and a host-vector system having a coryneform host bacterium, such as Corynebacterium glutamicum, and a vector which carries at least the sigC gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: August 30, 2001Date of Patent: May 2, 2006Assignee: Degussa AGInventors: Brigitte Bathe, Stephan Hans, Mike Farwick, Thomas Hermann, Walter Pfefferle
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Patent number: 7037690Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: March 26, 2003Date of Patent: May 2, 2006Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Patent number: 7011961Abstract: A method of producing L-threonine using a microorganism is provided, one or more copies of each of the phosphoenolpyruvate carboxylase gene and the threonine operon are additionally intedgrated into a particular site of the chromosomal DNA of the microorganism, whiel its inherent phophoenolpyruvate carboxylase gene and threonine operon remain.Type: GrantFiled: February 14, 2002Date of Patent: March 14, 2006Assignee: Cheil Jedang CorporationInventors: Kap-Soo Noh, Yeong-Chul Kim, Jae-Yong Park, Dai-Chul Kim, Jin-Ho Lee, Seung-Han Ok
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Patent number: 7008786Abstract: Disclosed are a Coryneform bacterium having resistance to an antibiotic, monensin, and producing L-lysine, and a process for producing L-lysine by a direct fermentation, which comprises culturing said microorganism in a fermentation medium, accumulating L-lysine in the resulting culture broth, and recovering L-lysine therefrom.Type: GrantFiled: December 28, 2001Date of Patent: March 7, 2006Assignee: Cheil Jedang CorporationInventors: Seong-Jun Kim, Kyung-Han Lee, Jin-Suck Sung, Sang-Jo Lim, Jae-Woo Jang
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Patent number: 6995000Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sigma factor M (sigM) gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in enhanced form and a vector which carries at least the sigM gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: August 31, 2001Date of Patent: February 7, 2006Assignee: DegussaInventors: Brigitte Bathe, Christine Bastuck, Mike Farwick, Thomas Hermann, Walter Pfefferle
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Patent number: 6979560Abstract: A bacterium belonging to the genus Escherichia and having an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing an expression amount of an L-amino acid excretion protein, and a method for producing the L-amino acid using the bacterium.Type: GrantFiled: December 13, 1999Date of Patent: December 27, 2005Assignee: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadievich Livshits, Natalia Pavlovna Zakataeva, Kazuo Nakanishi, Vladimir Veniaminovich Aleshin, Petr Vladimirovich Troshin, Irina Lyvovna Tokhmakova
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Patent number: 6972190Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the carbon starvation protein A (cstA) gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in enhanced form and a vector which carries at least the cstA gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: August 24, 2001Date of Patent: December 6, 2005Assignee: Degussa AGInventors: Bettina Möckel, Achim Marx, Walter Pfefferle, Mike Farwick, Thomas Hermann
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Patent number: 6960455Abstract: An amino acid such as threonine, homoserine, isoleucine, lysine, valine and tryptophan is produced using a bacterium belonging to the genus Escherichia which has been constructed from sucorse non-assimilative strain belonging to the genus Escherichia and which harbors sucrose non-PTS (phosphoenol pyruvate-dependent sucrose-6-phosphotransferase system) genes and has an ability to produce the amino acid.Type: GrantFiled: April 25, 2001Date of Patent: November 1, 2005Assignee: Ajinomoto Co., Inc.Inventors: Vitaliy Arkadyevich Livshits, Vera Georgievna Doroshenko, Sergei Vlsdimirovich Mashko, Valery Zavenovich Akhverdian, Yury Ivanovich Kozlov
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Patent number: 6953677Abstract: A novel rec-L-N-carbamoylase from Arthrobacter aurescens and its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.Type: GrantFiled: January 15, 2002Date of Patent: October 11, 2005Assignees: Degussa AG, Universitat StuttgartInventors: Josef Altenbuchner, Ralf Mattes, Markus Pietzsch, Christoph Syldatk, Anja Wiese, Burkard Wilms
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Patent number: 6939695Abstract: An isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the rpsL gene is present in enhanced form, as well as the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: February 15, 2002Date of Patent: September 6, 2005Assignee: Degussa AGInventors: Bettina Moeckel, Brigitte Bathe, Stefan Hans, Caroline Kreutzer, Thomas Hermann, Walter Pfefferle, Michael Binder
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Patent number: 6939694Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the citB gene, and a host-vector system having a coryneform host bacterium in which the citB gene is present in attenuated form and a vector which carries at least the citB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: August 31, 2001Date of Patent: September 6, 2005Inventors: Bettina Möckel, Thomas Hermann, Mike Farwick, Walter Pfefferle, Achim Marx
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Patent number: 6921651Abstract: The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enriching the desired L-amino acid in the medium or in the cells of the bacteria, and isolating the L-amino acid. Optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.Type: GrantFiled: March 18, 2002Date of Patent: July 26, 2005Assignee: Degussa AGInventors: Mike Farwick, Brigitte Bathe, Jennifer Brehme, Klaus Huthmacher
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Patent number: 6919190Abstract: The present invention provides a method of increasing the productivity of a microorganism by improving the assimilation of carbon dioxide. Specifically, the invention provides a polypeptide having phosphoenolpyruvate carboxylase activity which does not require acetyl coenzyme A for activation and is desensitized to feedback inhibition by aspartic acid, and to genes coding for this polypeptide. A gene encoding a PEP carboxylase that is not regulated by acetyl-CoA or aspartic acid can improve carbon flow from the three carbon intermediate PEP to the four carbon intermediate OAA, contribute to compounds derived from OAA, and increase amino acid biosynthesis. The invention further provides recombinant DNA molecules containing these genes, bacteria transformed with these genes, and a method of producing amino acids using the transformed bacteria.Type: GrantFiled: October 18, 2001Date of Patent: July 19, 2005Assignee: Archer-Daniels-Midland CompanyInventors: P. John Rayapati, Corey M. Crafton
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Patent number: 6916637Abstract: The invention relates to a fermentation process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out: a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms at least the pckA gene and/or the open reading frames yjfA and ytfP are, individually or jointly, attenuated and, in particular, switched off, b) enrichment of the L-amino acid in the medium or in the bacterial cells, and c) isolation of the L-amino acid.Type: GrantFiled: September 27, 2001Date of Patent: July 12, 2005Assignee: Degussa AGInventors: Mechthild Rieping, Christine Bastuck, Thomas Hermann, Georg Thierbach
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Patent number: 6916636Abstract: The invention relates to polynucleotides corresponding to the oxyR gene from Corynebacterium glutamicum and which encode a OxyR transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having OxyR transcriptional regulator activity.Type: GrantFiled: August 27, 2001Date of Patent: July 12, 2005Assignee: Degussa AGInventors: Achim Marx, Mike Farwick, Thomas Hermann, Natalie Schischka, Brigitte Bathe
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Patent number: 6913909Abstract: The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out: a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes, b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, and c) Isolation of the L-threonine.Type: GrantFiled: September 27, 2001Date of Patent: July 5, 2005Assignee: Degussa AGInventors: Petra Ziegler, Lothar Eggeling, Hermann Sahm, Georg Thierbach
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Patent number: 6913910Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising: a) polynucleotide that is at least 70% identical to a polynucleotide coding for a polypeptide that contains the amino acid sequence of SEQ ID No. 2, b) polynucleotide coding for a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID No.2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequence of a), b) or c), and processes for the fermentative production of L-amino acids by enhancement of the glk-gene coding for the enzyme glucokinase.Type: GrantFiled: July 18, 2002Date of Patent: July 5, 2005Assignee: Degussa AGInventors: Bettina Möckel, Walter Pfefferle
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Patent number: 6913908Abstract: The present invention relates lo an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group c insisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of(a) or (b), and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.Type: GrantFiled: August 24, 2001Date of Patent: July 5, 2005Assignee: Degussa AGInventors: Bettina Möckel, Thoams Hermann, Mike Farwick, Michael Binder, Walter Pfefferle
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Patent number: 6902916Abstract: The present invention relates to polynucleotides corresponding to the lysR1 gene and which encode a LysR1 transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LysR1 transcriptional regulator activity.Type: GrantFiled: July 13, 2001Date of Patent: June 7, 2005Assignee: Degussa AGInventors: Bettina Moeckel, Mike Farwick, Thomas Hermann, Caroline Kreutzer, Walter Pfefferle
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Patent number: 6902915Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).Type: GrantFiled: February 7, 2003Date of Patent: June 7, 2005Assignee: Daicel Chemical Industries, Ltd.Inventors: Shinji Tokuyama, Akinobu Matsuyama
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Patent number: 6893860Abstract: The present invention relates to methods and microorganisms for producing L-threonine. In particular, the present invention relates to the production of L-threonine using microorganisms, and Escherichia coli strains in particular, which require L-methionine for growth and are L-isoleucine-leaky, are resistant to ?-methylserine, diaminosuccinic acid, L-glutamic acid, L-threonine, medium containing 60% of L-threonine fermentation mother liquid, azetidine and dehydroproline, and which are susceptible to fluoropyruvate.Type: GrantFiled: August 26, 1999Date of Patent: May 17, 2005Assignee: Daesang CorporationInventors: Hyo Hoon Lee, Jae Chun Han, Tae Man Jung
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Patent number: 6893848Abstract: The present invention relates to a novel aspartokinase derived from a Coryneform bacterium; a DNA encoding the enzyme; a recombinant DNA containing the above DNA; a Coryneform bacterium having the above recombinant DNA or a Coryneform bacterium having the DNA on its chromosome; and a process for producing L-lysine by culturing the above microorganism. Construction has been successfully made of a DNA encoding an aspartokinase freed from concerted feedback inhibition by L-lysine and L-threonine derived from a Corynebacterium and has a nucleotide sequence encoding an amino acid sequence wherein the amino acid residue at position 311 is an amino acid other than Thr in the amino acid sequence shown by SEQ ID NO: 18.Type: GrantFiled: April 14, 2000Date of Patent: May 17, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Haruhiko Yokoi, Junko Ohnishi, Keiko Ochiai, Yoshiyuki Yonetani, Akio Ozaki
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Patent number: 6890744Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the sigD gene, and a host-vector system having a coryneform host bacterium in which the sigD gene is present in enhanced form and a vector which carries at least the sigD gene according to SEQ ID No: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: August 30, 2001Date of Patent: May 10, 2005Assignee: Degussa AGInventors: Brigitte Bathe, Caroline Kreutzer, Monika Martens, Mike Farwick, Thomas Hermann, Walter Pfefferle
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Patent number: 6887691Abstract: A bacterium which has an ability to produce an amino acid and in which a novel gene (rhtB) coding for a protein having an activity of making a bacterium having the protein L-homoserine-resistant is enhanced, is cultivated in a culture medium to produce and accumulate the amino acid in the medium, and the amino acid is recovered from the medium.Type: GrantFiled: May 3, 2001Date of Patent: May 3, 2005Assignee: Ajinomoto Co., Inc.Inventors: Vitaly Arkadievich Livshits, Natalya Pavlovna Zakataeva, Vladimir Venyamiovich Aleoshin, Alla Valentinovna Balareova, Irina Lvovna Tokhmakova
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Patent number: 6884606Abstract: The present invention concerns an anaplerotic enzyme from Corynebacterium glutamicum which replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.Type: GrantFiled: January 15, 2002Date of Patent: April 26, 2005Assignee: Massachusetts Institute of TechnologyInventors: Anthony J. Sinskey, Philip A. Lessard, Laura B. Willis
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Patent number: 6878533Abstract: An Escherichia coil mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library of Bacillus methanolicus, a transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.Type: GrantFiled: August 9, 2002Date of Patent: April 12, 2005Assignee: Ajinomoto Co., Inc.Inventors: Nobuharu Tsujimoto, Hisashi Yasueda, Yoshio Kawahara, Shinichi Sugimoto
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Patent number: 6872553Abstract: The invention relates to isolated nucleotide sequences from Coryneform bacteria which code for the pck gene encoding the enzyme phosphoenol pyruvate carboxykinase (PEP carboxykinase). The invention also relates a process for the fermentative preparation of L-amino acids, in particular L-lysine, L-threonine, and L-glutamate by attenuation of the pck gene.Type: GrantFiled: May 6, 2002Date of Patent: March 29, 2005Assignee: Degussa AGInventors: Bernhard Eikmanns, Christian Riedel, Hermann Sahm, Bettina Mockel
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Patent number: 6861246Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.Type: GrantFiled: March 19, 2001Date of Patent: March 1, 2005Assignee: Degussa AGInventors: Caroline Kreutzer, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
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Publication number: 20040265956Abstract: The present invention describes a method for producing a target substance using a microorganism comprising culturing an Escherichia bacterium in a medium to produce and accumulate the target substance in the medium or the bacterium, and collecting the target substance. More specifically, the bacterium of the present invention can be a strain in which the fis gene on bacterial chromosome is disrupted so that the FIS protein does not function normally in the bacterium.Type: ApplicationFiled: November 6, 2003Publication date: December 30, 2004Inventors: Rie Takikawa, Akira Imaizumi, Kazuhiko Matsui, Hiroyuki Kojima
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Publication number: 20040259215Abstract: The invention relates to novel nucleic acid molecules, to the use thereof for constructing genetically improved microorganisms and to methods for preparing fine chemicals, in particular amino acids, with the aid of said genetically improved microorganisms.Type: ApplicationFiled: May 4, 2004Publication date: December 23, 2004Inventors: Oskar Zelder, Markus Pompejus, Hartwig Schroder, Burkhard Kroger, Corina Klopprogge, Gregor Haberhauer
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Patent number: 6830903Abstract: The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacterial cells are greater than yields from unaltered bacterial cells. The invention also relates to a gene encoding phosphoglucoisomerase.Type: GrantFiled: July 21, 2000Date of Patent: December 14, 2004Assignee: Archer-Daniels-Midland CompanyInventors: Michael R. O'Donohue, Paul D. Hanke
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Publication number: 20040248264Abstract: The invention relates to novel nucleic acid molecules, to the use thereof for constructing genetically improved microorganisms and to methods for preparing fine chemicals, in particular amino acids, with the aid of said genetically improved microorganisms.Type: ApplicationFiled: May 3, 2004Publication date: December 9, 2004Inventors: Oskar Zelder, Markus Pompejus, Hartwig Schroder, Burkhard Kroger, Corinna Klopprogge, Gregor Haberhauer