Lysine; Diaminopimelic Acid; Threonine; Valine Patents (Class 435/115)
  • Novel lysine decarboxylase gene and method for producing L-lysine
    Publication number: 20040229311
    Abstract: A Methylophilus bacterium in which a gene having a nucleotide sequence identical to a DNA cording for a protein defined in the following (A) or (B) or a gene having homology to the DNA in such a degree that homologous recombination with the DNA occurs is disrupted, thereby expression of the gene is suppressed and the intracellular lysine decarboxylase activity is reduced or eliminated is cultured in a medium containing methanol as a major carbon source to produce and accumulate L-lysine in culture and the L-lysine is collected from the culture: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion or addition of one or several amino acid residues and has a lysine decarboxylase activity.
    Type: Application
    Filed: February 25, 2004
    Publication date: November 18, 2004
    Inventors: Seiko Hirano, Hisashi Yasueda
  • Process for the isolation and purification of mevinolin
    Patent number: 6812007
    Abstract: In a process for preparing mevinolin by fermentation of a biomass in a fermentation liquor, which includes dissolving mevinolin from the biomass into the fermentation liquor, and separating the biomass from the fermentation liquor to obtain a separated fermentation liquor, separating the mevinolin from the separated fermentation liquor, and recovering the end product, the improvement which comprises carrying out the dissolving at a pH between 7.5 and about 10, and the separating of the mevinolin is carried out at a pH between about 4.5 and about 1.
    Type: Grant
    Filed: April 19, 2000
    Date of Patent: November 2, 2004
    Inventors: Vilmos Kéri, Irma Högye, Antónia Jekkel, Ilona Bagdi, Gábor Ambrus, Attila Jakab, Attila Andor, Lajos Deák, István Szabó, János Bálint, Zsuzsanna Scheidl, Etelka Deli, Gyula Horváth, Csaba Szabó, Ildikó Láng, Imre Székely, Imre Moravcsik, Vera Kovács, Szabolcs Mátyás, Zsuzsanna Sztáray, László Eszenyi, Éva Ilköy
  • Method for producing L-lysine using methanol-utilizing bacterium
    Publication number: 20040214296
    Abstract: L-Lysine is produced by culturing a methanol-utilizing bacterium which requires L-methionine for its growth and has an ability to produce L-lysine in a medium containing methanol as a main carbon source to produce and accumulate L-lysine in culture and collecting the L-lysine from the culture.
    Type: Application
    Filed: January 21, 2004
    Publication date: October 28, 2004
    Inventors: Takayuki Asahara, Seiko Hirano, Hisashi Yasueda
  • Process for the preparation of L-amino acids by fermentation and nucleotide sequences coding for the accDA gene
    Patent number: 6808914
    Abstract: The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: October 26, 2004
    Assignee: Degussa AG
    Inventors: Yvonne Tilg, Bernhard Eikmanns, Lothar Eggeling, Hermann Sahm, Bettina Möckel
  • Nucleotide sequences which encode the pfk gene
    Patent number: 6806068
    Abstract: The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been derived from Corynebacterium glutamicum.
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: October 19, 2004
    Assignee: Degussa AG Dusseldorf
    Inventors: Bettina Möckel, Walter Pfefferle
  • Nucleotide sequences which code for the RodA protein
    Patent number: 6777206
    Abstract: The invention provides nucleotide sequences from Coryneform bacteria which code for the RodA protein and a process for the fermentative preparation of amino acids using bacteria in which the rodA gene is enhanced.
    Type: Grant
    Filed: September 12, 2001
    Date of Patent: August 17, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Walter Pfefferle, Brigitte Bathe
  • Method for producing L-amino acid using methylotroph
    Publication number: 20040146974
    Abstract: A DNA encoding for a mutant of LysE protein, or a homologous protein thereof, of a coryneform bacterium, wherein the mutant, when introduced into a methanol-assimilating bacterium imparts resistance to L-lysine analogue. The DNA encoding for a mutant of LysE protein, or a homologous protein thereof, is introduced into a methanol-assimilating bacterium to improve L-lysine and L-arginine productivity of the methanol-assimilating bacterium.
    Type: Application
    Filed: November 20, 2003
    Publication date: July 29, 2004
    Inventors: Yoshiya Gunji, Hisashi Yasueda
  • Nucleotide sequences coding for the glbO gene
    Patent number: 6759218
    Abstract: The present invention provides an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the glbO gene which codes for the haemoglobin-like protein and the use of the above polynucleotides as a primer or hybridization probe.
    Type: Grant
    Filed: May 7, 2002
    Date of Patent: July 6, 2004
    Assignee: Degussa-AG
    Inventors: Bettina Mockel, Achim Marx, Walter Pfefferle
  • Polynucleotides encoding a feedback resistant aspartokinase from Corynebacterium
    Publication number: 20040126854
    Abstract: The invention provides methods to increase the production of an amino acid from Corynebacterium species by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome. The invention also provides novel processes for the production of an amino acid by way of the amplification of amino acid biosynthetic pathway genes in a host cell chromosome and/or by increasing promoter strength. In a preferred embodiment, the invention provides processes to increase the production of L-lysine in Corynebacterium glutamicum by way of the amplification of L-lysine biosynthetic pathway genes in a host cell chromosome. The invention also provides novel isolated nucleic acid molecules for L-lysine biosynthetic pathway genes of Corynebacterium glutamicum.
    Type: Application
    Filed: February 4, 2004
    Publication date: July 1, 2004
    Applicant: Archer-Daniels-Midland Company
    Inventors: Paul D. Hanke, Lhing-Yew Li-D'Elia, Holly J. Walsh, Corey M. Crafton, P. John Rayapati
  • L-lysine-producing corynebacteria and process for the preparation of L-lysine
    Patent number: 6746855
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Grant
    Filed: January 8, 2003
    Date of Patent: June 8, 2004
    Assignees: Dégussa-Hüls Aktiengesellschaft, Forschungszentrum Jülich GmbH
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum
    Patent number: 6746854
    Abstract: This invention relates to novel polynucleotide sequences encoding the histidine kinase luminescence expression sensor (luxS) gene from Corynebaclerium glutamicum, probes to the novel polynucleotide sequences encoding the luxS gene, vector and host cells containing the novel luxS polynucleotide sequences, the encoded Lux S polypeptide, and a process for the fermentative preparation of amino acids using bacteria which the luxS gene is attenuated.
    Type: Grant
    Filed: August 1, 2001
    Date of Patent: June 8, 2004
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Caroline Kreutzer, Achim Marx, Walter Pfefferle
  • Mutant ilvH gene and method for producing L-valine
    Patent number: 6737255
    Abstract: The present invention provides an isolated DNA molecule encoding a small subunit of acetohydroxy acid synthase isozyme III originating from Escherichia coli and mutants of Escherichia coli acetohydroxy acid synthase isozyme III, which are free from inhibition by L-valine an can catalyze the conversion of: (a) pyruvate to &agr;-acetolactate and (b) &agr;-ketobutyrate and pyruvate to &agr;-aceto-a-hydroxybutyrate. The present invention also provides methods for producing L-valine by fermentation of a bacterium harboring the novel DNA molecule and/or expressing the mutant acetohydroxy acid synthase isozyme III.
    Type: Grant
    Filed: January 18, 2001
    Date of Patent: May 18, 2004
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vitaliy Arkadyevich Livshits, Vera Georgievna Doroshenko, Nataliya Vasilyevna Gorshkova, Alla Valentinovna Belaryeva, Lirina Valeryevna Ivanovskaya, Evgeni Moiseevich Khourges, Valery Zavenovich Akhverdian, Mikhail Markovich Gusyatiner, Yury Ivanovich Kozlov
  • Method for preventing photooxidation or air oxidation in food, pharmaceuticals and plastics
    Patent number: 6730518
    Abstract: Disclosed is a method of reducing photooxidation or air oxidation in susceptible materials such as food, plastics or pharmaceuticals comprising mixing the material with an antioxidation composition comprising at least one amino acid, at least one metal ion, and at least one carboxylic acid in an amount effective to reduce photooxidation in the material.
    Type: Grant
    Filed: September 25, 2000
    Date of Patent: May 4, 2004
    Assignee: Natura, Inc.
    Inventors: Joel I. Dulebohn, Ronald J. Carlotti
  • Process for the production of L-lysine using coryneform bacteria
    Publication number: 20040067561
    Abstract: A process for the production of L-lysine, comprising:
    Type: Application
    Filed: July 30, 2003
    Publication date: April 8, 2004
    Applicant: Degussa AG
    Inventors: Brigitte Bathe, Caroline Reynen, Walter Pfefferle
  • Process for the production of L-lysine using coryneform bacteria
    Publication number: 20040067562
    Abstract: A process for the production of L-lysine, in which the following steps are carried out:
    Type: Application
    Filed: July 31, 2003
    Publication date: April 8, 2004
    Applicant: Degussa AG
    Inventors: Brigitte Bathe, Stephan Hans, Walter Pfefferle
  • Process for the preparation of L-amino acids using a gene encoding 6-phosphogluconate dehydrogenase
    Publication number: 20040063181
    Abstract: The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid produced by the fermenting in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an overexpressed gene encoding 6-phosphogluconate dehydrogenase and a decreased or switched off gene encoding pyruvate oxidase. The L-amino acid may be L-lysine, L-threonine, L-isoleucine or L-tryptophan.
    Type: Application
    Filed: October 17, 2003
    Publication date: April 1, 2004
    Applicants: Degussa-Huls Aktiengasellschaft, National University of Ireland
    Inventors: L. K. Duncan, Ashling McCormack, Cliona Stapleton, Kevin Burke, Bettina Mockel
  • Nucleotide sequences which code for the eno gene
    Patent number: 6713289
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Grant
    Filed: May 21, 2001
    Date of Patent: March 30, 2004
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Thomas Hermann, Alfred Pühler, Jörn Kalinowski, Brigitte Bathe
  • Coryneform bacteria which produce chemical compounds II
    Publication number: 20040043458
    Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: February 5, 2003
    Publication date: March 4, 2004
    Applicant: Degussa AG
    Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
  • Nucleotide sequences which encode plsC gene
    Patent number: 6680186
    Abstract: The present invention relates to polynucleotides corresponding to the plsC gene and which encode 1-acyl-SN-glycerol-3-phosphate acyltransferase, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having 1-acyl-SN-glycerol-3-phosphate acyltransferase activity.
    Type: Grant
    Filed: April 11, 2001
    Date of Patent: January 20, 2004
    Assignees: Degussa AG, Forschungszentrum Juelich GmbH
    Inventors: Madhavan Nampoothiri, Brigitte Bathe, Lothar Eggeling, Hermann Sahm
  • Nucleotide sequences encoding the ptsH gene
    Publication number: 20040005675
    Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising
    Type: Application
    Filed: January 8, 2001
    Publication date: January 8, 2004
    Inventors: Mike Farwick, Bettina Mockel, Walter Pfefferle
  • Glucoamylase variants
    Publication number: 20040002142
    Abstract: The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
    Type: Application
    Filed: April 23, 2003
    Publication date: January 1, 2004
    Applicant: Novozymes A/S
    Inventors: Bjarne Ronfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
  • Polynucleotide encoding a diaminopimelate epimerase from Corynebacterium glutamicum
    Patent number: 6670156
    Abstract: The present application is directed to a diaminopimelate epimerase from Corynebacterium glutamicum and to polynucleotides encoding this enzyme. The gene has been given the designation “dapF.
    Type: Grant
    Filed: October 27, 1999
    Date of Patent: December 30, 2003
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Brigitte Bathe, Jorn Kalinowski, Oliver Kirchner, Michael Hartmann, Alfred Pühler
  • Nucleotide sequences encoding the ptsH gene
    Publication number: 20030224499
    Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising
    Type: Application
    Filed: March 29, 2001
    Publication date: December 4, 2003
    Inventors: Mike Farwick, Bettina Mockel, Walter Pfefferle
  • Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
    Patent number: 6653111
    Abstract: The present invention provides a method for deriving an E. coli strain which produces L-theronine from E.coli strain VNIIgenetika 472T23 by transducing with bacteriophage P1 which bears a transposon.
    Type: Grant
    Filed: July 13, 2001
    Date of Patent: November 25, 2003
    Assignee: Ajinomoto Co., Inc.
    Inventors: Vladimir Georgievich Debabov, Jury Ivanovich Kozlov, Evgeny Moiseevich Khurges, Vitaly Arkadievich Livshits, Nelli Isaakovna Zhdanova, Mikhail Markovich Gusyatiner, Alexandr Konstantinovich Sokolov, Tatyana Alexandrovna Bachina, Nikolai Kazimirovich Yankovsky, Jury Dmitrievich Tsygankov, Andrei Jurievich Chistoserdov, Tatyana Grigorievna Plotnikova, Irina Olegovna Shakalis, Alla Valentinovna Belareva, Raisa Alexandrovna Arsatiants, Albert Fedorovich Sholin, Tamara Mikhailovna Pozdnyakova
  • Method and deregulated enzyme for threonine production
    Patent number: 6649379
    Abstract: Mutagenesis of the gene encoding homoserine dehydrogenase (hom) for production of the amino acid threonine is described. The mutation causes an alteration in the carboxy terminus of the enzyme that interferes with end-product inhibition by threonine. The lack of end-product inhibition causes an overproduction of threonine.
    Type: Grant
    Filed: November 1, 1991
    Date of Patent: November 18, 2003
    Assignee: Massachusetts Institute of Technology
    Inventors: John A. C. Archer, Maximillian T. Follettie, Anthony J. Sinskey
  • Process for the preparation of L-amino acids with amplification of the zwf gene
    Publication number: 20030199045
    Abstract: The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
    Type: Application
    Filed: March 6, 2002
    Publication date: October 23, 2003
    Inventors: Kevin Burke, Hermann Sahm, Lothar Eggeling, Bernd Moritz, L. K. Dunican, Ashling McCormack, Cliona Stapelton, Bettina Mockel, Georg Thierbach, Rita Dunican
  • Nucleotide sequences which code for the zwa1 gene
    Patent number: 6632644
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: December 8, 2000
    Date of Patent: October 14, 2003
    Assignee: DeGussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Method for isolating a target biological material, capture phase, detecting phase and reagent containing them
    Patent number: 6632615
    Abstract: The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognizing or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues, the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.
    Type: Grant
    Filed: April 21, 1999
    Date of Patent: October 14, 2003
    Assignee: Bio Merieux
    Inventors: Francois Mallet, Thierry DeLair, Catherine Ladaviere, Armelle Novelli-Rousseau, Marie-Helene Charles
  • Process for the preparation of L-amino acids with amplification of the zwf gene
    Publication number: 20030175911
    Abstract: The invention relates to a process for the preparation of L-amino acids by the fermentation of coryneform bacteria. The process involves: fermenting an L-amino acid-producing bacteria in which at least the zwf gene is amplified; concentrating the L-amino acid in the medium or in the cells of the bacteria; and isolating the L-amino acid produced.
    Type: Application
    Filed: January 3, 2003
    Publication date: September 18, 2003
    Inventors: Stephen Hans, Brigitte Bathe, Alexander Reth, Georg Thierbach, Caroline Kreutzer, Bettina Mockel
  • Method for modifying the genome of corynebacteria
    Publication number: 20030170775
    Abstract: The invention relates to a process for producing corynebacteria comprising one or more modified genomic sequences, where a vector is used which does not replicate in corynebacteria and whose nucleic acid is not recognized by corynebacteria as foreign.
    Type: Application
    Filed: March 12, 2003
    Publication date: September 11, 2003
    Inventors: Markus Pompejus, Hartwig Schroder, Burkhard Kroger, Oskar Zelder
  • Nucleotide sequences coding for the glbO gene
    Publication number: 20030166173
    Abstract: The present invention provides an isolated polynucleotide containing a polynucleotide sequence selected from the group
    Type: Application
    Filed: May 7, 2002
    Publication date: September 4, 2003
    Inventors: Bettina Mockel, Achim Marx, Walter Pfefferle
  • Methods for producing L-amino acids
    Publication number: 20030166174
    Abstract: A methane-utilizing microorganism capable of producing L-amino acid, for example, bacteria belonging to type I, type X or type II in the taxonomic categorization methane-utilizing bacteria such as Methylomonas albus, Methylococcus capsulatus and Methylosinus trichosporium, is cultivated in a culture medium in contact with gas containing methane which is the main source of carbon, to allow the L-amino acid to be produced and accumulated in the medium, and the L-amino acid is collected from the medium.
    Type: Application
    Filed: December 4, 2002
    Publication date: September 4, 2003
    Applicant: Ajinomoto Co., Inc.
    Inventors: Yukiko Ono, Hisashi Yasueda, Yoshio Kawahara, Shinichi Sugimoto
  • L-lysine-producing corynebacteria and process for the preparation of L-lysine
    Publication number: 20030162269
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Application
    Filed: January 8, 2003
    Publication date: August 28, 2003
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Dry granulated product containing L-lysine as main component
    Publication number: 20030152633
    Abstract: Hydrochloric acid, sulfuric acid or an L-lysine solution having an equivalent ratio of anion/L-lysine higher than 0.95 is added to a raw material L-lysine solution having an equivalent ratio of anion/L-lysine lower than 0.68 to adjust the equivalent ratio of anion/L-lysine of the raw material solution to be in the range of 0.68 to 0.95, and the obtained L-lysine solution or a concentrate thereof is granulated and dried to obtain a dry granulated product having a high L-lysine content and showing low caking property and low hygroscopic property.
    Type: Application
    Filed: January 24, 2003
    Publication date: August 14, 2003
    Applicant: AJINOMOTO CO., INC.
    Inventors: Takeshi Kushiki, Junko Morikawa, Kazuhiko Hasegawa
  • Nucleotide sequences that code for the rplK gene and methods of use thereof
    Publication number: 20030148476
    Abstract: An isolated polynucleotide from coryneform bacteria containing a polynucleotide sequence selected from the group:
    Type: Application
    Filed: November 25, 2002
    Publication date: August 7, 2003
    Inventors: Lutz Wehmeier, Andreas Tauch, Alfred Puhler, Jorn Kalinowski, Bettina Mockel
  • Nucleotide sequences which code for the opcA gene
    Publication number: 20030138917
    Abstract: The present invention relates to polynucleotides that encode proteins having OpcA enzymatic activity. These polynucleotides can be used for increasing lysine biosynthesis in Coryneform glutamicum.
    Type: Application
    Filed: May 3, 2002
    Publication date: July 24, 2003
    Inventors: L. K. Dunican, Rita Dunican, Ashling McCormack, Cliona Stapleton, Kevin Burke, Bernd S. Moritz, Lothar Eggeling, Hermann Sahm, Bettina Mockel, Anke Weissenborn
  • Microorganism producing l-lysine and processes for producing l-lysine using the same
    Publication number: 20030124688
    Abstract: Disclosed are a Coryneform bacterium having resistance to an antibiotic, monensin, and producing L-lysine, and a process for producing L-lysine by a direct fermentation, which comprises culturing said microorganism in a fermentation medium, accumulating L-lysine in the resulting culture broth, and recovering L-lysine therefrom.
    Type: Application
    Filed: August 26, 2002
    Publication date: July 3, 2003
    Inventors: Seong-Jun Kim, Kyung-Han Lee, Jin-Suck Sung, Sang-Jo Lim, Jae-Woo Jang
  • Method for producing L-lysine or L-arginine by using methanol assimilating bacterium
    Publication number: 20030124687
    Abstract: A DNA encoding a variant of a protein, having a loop region and six hydrophobic helixes and involved in excretion of L-lysine to outside of a cell, wherein the DNA encodes a mutant protein not containing the loop region that is contained in a wild-type protein and facilitates excretion of L-lysine, L-arginine or both of these L-amino acids to outside of a cell of a methanol assimilating bacterium when the DNA is introduced into the bacterium, specifically lysE24, is introduced into a methanol assimilating bacterium such as Methylophilus bacteria to improve L-amino acid productivity, especially L-lysine and L-arginine productivities.
    Type: Application
    Filed: June 11, 2002
    Publication date: July 3, 2003
    Applicant: Ajinomoto Co., Inc.
    Inventors: Yoshiya Gunji, Hisashi Yasueda
  • Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
    Patent number: 6586214
    Abstract: The present invention is directed to isolated polynucleotides coding for phosphoglucose isomerase (pgi) from coryneform bacteria. In addition, the invention includes methods for increasing the metabolic flux through pentose phosphate cycle of bacteria by reducing or eliminating the activity of pgi. These methods may be used to increase the fermentative production of nucleotides, vitamins and amino acids.
    Type: Grant
    Filed: September 15, 1999
    Date of Patent: July 1, 2003
    Assignee: Degussa AG
    Inventors: L. K. Dunican, Ashling McCormack, Cliona Stapleton, Kevin Burke, Michael O'Donohue, Achim Marx, Bettina Mockel
  • Method for producing target substance
    Publication number: 20030119155
    Abstract: In a method for producing a target substance by using a microorganism comprising culturing a microorganism having an ability to produce the target substance in a medium to produce and accumulate the target substance in the medium or cells of the microorganism and collecting the target substance from the medium or the cells of the microorganism, there are used, as the microorganism, a microorganism to which a methanol dehydrogenase gene is introduced, of which activities of hexulose phosphate synthase and phosphohexuloisomerase are enhanced and which is modified so that an ability to utilize methanol should be imparted or enhanced, and there is used a medium containing methanol as a carbon source.
    Type: Application
    Filed: September 5, 2002
    Publication date: June 26, 2003
    Applicant: AJINOMOTO CO., INC.
    Inventors: Hisashi Yasueda, Ryo Takeshita
  • Process for the preparation of L-amino acids using a gene encoding 6-phosphogluconate dehydrogenase
    Publication number: 20030119154
    Abstract: The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid produced by the fermenting in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an overexpressed gene encoding 6-phosphogluconate dehydrogenase and a decreased or switched off gene encoding pyruvate oxidase. The L-amino acid may be L-lysine, L-threonine, L-isoleucine or L-tryptophan.
    Type: Application
    Filed: February 20, 2002
    Publication date: June 26, 2003
    Inventors: L. K. Dunican, Ashling McCormack, Cliona Stapelton, Kevin Burke, Bettina Mockel
  • Novel nucleotide sequences coding the citE gene
    Publication number: 20030113879
    Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group
    Type: Application
    Filed: June 6, 2001
    Publication date: June 19, 2003
    Inventors: Mike Farwick, Klaus Huthmacher, Achim Marx, Brigitte Bathe, Walter Pfefferle
  • Method for producing fermentative product and stress-resistant microorganism
    Patent number: 6579699
    Abstract: A method for producing a fermentative product by utilizing a microorganism, the method comprising culturing the microorganism in a medium to produce and accumulate the fermentative product in the medium, and collecting the fermentative product, wherein the microorganism expresses a heat shock protein derived from a hyperthermophilic archaeon strain KOD-1 in a cell of the microorganism by introduction of a gene coding for the heat shock protein.
    Type: Grant
    Filed: August 10, 1999
    Date of Patent: June 17, 2003
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yoshimi Kikuchi, Osamu Kurahashi, Kenzo Yokozeki, Takashi Tanaka
  • Process for the fermentative preparation of L-amino acids with amplification of the tkt gene
    Publication number: 20030109014
    Abstract: The invention relates to a process for the preparation of L-amino acids by the fermentation of coryneform bacteria that over-express a gene encoding transketolase.
    Type: Application
    Filed: May 14, 2002
    Publication date: June 12, 2003
    Inventors: Kevin Burke, L. K. Dunican, Rita Duncian, Ashling McCormack, Cliona Stapleton, Bettina Mockel, Georg Thierbach
  • Process for the fermentative preparation of metabolic products and for the nucleotide sequences encoding for the sod gene
    Patent number: 6569650
    Abstract: The present invention is directed to nucleotide sequences coding for the superoxide dismutase (sod) gene from Corynebacterium melassecola. It includes processes for the fermentative preparation of nucleotides, vitamins and L-amino acids using coryneform bacteria in which the sod gene is amplified.
    Type: Grant
    Filed: August 13, 1999
    Date of Patent: May 27, 2003
    Assignee: Degussa AG
    Inventors: Muriel Merkamm, Armel Guyonvarch, Achim Marx
  • Fermentation process for the preparation of L-threonine
    Patent number: 6562601
    Abstract: The invention relates to a process for the fermentative preparation of L-threonine in which an L-threonine-producing microorganism of the Enterobacteriaceae family is cultured by the feed process, and a portion of the fermentation broth is then separated off in order to be utilized for inoculation of further media.
    Type: Grant
    Filed: August 24, 2001
    Date of Patent: May 13, 2003
    Assignee: Degussa AG
    Inventors: Thomas Hermann, Mechthild Rieping
  • Process for the fermentative production of L-lysine using coryneform bacteria
    Publication number: 20030087400
    Abstract: The invention relates to a process for the production of L-amino acids, and in particular L-lysine, in which the following steps are performed,
    Type: Application
    Filed: June 25, 2002
    Publication date: May 8, 2003
    Inventors: Bettina Mockel, Walter Pfefferle, Sven Brand, Alfred Puhler, Jorn Kalinowski, Brigitte Bathe
  • Controlling protein levels in eucaryotic organisms
    Patent number: 6559280
    Abstract: The invention relates to novel compounds comprising a ubiquitination recognition element and a protein binding element. The invention also relates to the use of said compounds for modulating the level and/or activity of a target protein. The compounds are useful for the treatment of disease such as infections, inflammatory conditions, cancer and genetic diseases. The compounds are also useful as insecticides and herbicides.
    Type: Grant
    Filed: June 14, 2001
    Date of Patent: May 6, 2003
    Assignee: Proteinix, Inc.
    Inventors: John H. Kenten, Steven F. Roberts
  • Method for producing target substance by fermentation
    Publication number: 20030077764
    Abstract: In a method for producing a target substance by utilizing a microorganism, which comprises culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture, there is used, as the microorganism, a mutant strain or recombinant strain of a microorganism in which maltose assimilation is controlled by an interaction between IIAGlc protein of glucose PTS and a protein involved in non-PTS uptake of maltose, and the interaction between the IIAGlc protein and the protein involved in non-PTS uptake of maltose is reduced or eliminated in the mutant strain or recombinant strain.
    Type: Application
    Filed: April 15, 2002
    Publication date: April 24, 2003
    Applicant: AJINOMOTO CO., INC
    Inventors: Nobuharu Tsujimoto, Tomoko Suzuki, Hisao Ito
  • Method for purification of amino acid containing solutions by electrodialysis
    Patent number: 6551803
    Abstract: A process is described for purifying an amino acid-containing solution by means of electrodialysis, wherein an amino acid-containing solution is employed which is obtained from the fermentation for producing at least one amino acid.
    Type: Grant
    Filed: May 1, 2002
    Date of Patent: April 22, 2003
    Assignee: BASF Aktiengesellschaft
    Inventors: Andreas Fischer, Christoph Martin, Jürgen Müller