Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the dep67 gene, and a host-vector system having a coryneform host bacterium in which the dep67 gene is present in attenuated form and a vector which carries at least the dep67 gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the citB gene, and a host-vector system having a coryneform host bacterium in which the citB gene is present in attenuated form and a vector which carries at least the citB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of

Abstract: Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

Abstract: Isolated polynucleotides containing at least one nucleic acid sequence selected from the group

Abstract: The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine.

Abstract: The invention relates to polynucleotides that contain poLynucleotide sequences coding for the genes sucC and sucD, selected from the group

Abstract: The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.

Abstract: The invention relates to polynucleotides corresponding to the oxyR gene and which encode a OxyR transcriptional regulator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having OxyR transcriptional regulator activity.

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract: The invention provides a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which the subunit carrying the biotin-carboxyl carrier protein domain and the biotin-carboxylase domain of the nucleotide sequence encoding the enzyme acetyl-CoA carboxylase (accBC gene) is amplified, in particular is overexpressed.

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the ccsB gene, and a host-vector system having a coryneform host bacterium in which the ccsB gene is present in attenuated form and a vector which carries at least the ccsB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: This invention relates to a novel bacterial ribonucleoprotein complex and the component parts thereof More specifically, this invention relates to RNase P isolated from S. pneumoniae and the use of RNase P or components thereof in screens for the identification of antimicrobial compounds and to the use of such compounds in therapy.

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the ppsA gene, and a host-vector system having a coryneform host bacterium in which the ppsA gene is present in attenuated form and a vector which carries at least the ppsA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: The invention relates to a genetically modified coryneform bacterium, the cma gene of which is amplified, and an isolated polynucleotide which codes for cyclopropane-mycolic acid synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the cma gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.

Abstract: The invention relates to a genetically modified coryneform bacterium, the fadD15 gene of which is amplified, and an isolated polynucleotide which codes for acyl-CoA synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the fadD15 gene in the bacteria and the use of the polynucleotide as a primer or hybridization probe.

Abstract: A process for stereoselectively inverting a chiral center of a chemical compound is disclosed. The process first consists of forming a mixture of the chemical comopund, an enzymatic system, and a metal catalyst. Next, the process stereoselectively dehydrogenates a group attached to the chiral center with the enzymatic system in the presence of an oxidant to produce a dehydrogenated group, Lastly, the process hydrogenates the dehydrogenated group with the metal catalyst in the presence of a hydrogen source to stereoselectively invert the chiral center of the chemical compound.

Abstract: An isolated nucleic acid that encodes glycerol-3-phosphate dehydrogenase from coryneform bacteria, variants, homologs and fragments thereof. Hybridization probes and primers, vectors and host cells comprising such sequences. Coryneform bacterium with an enhanced ability to express glycerol-3-phosphate dehyrogenase. Methods of fermentative production of L-amino acids using coryneform bacteria having enhanced expression of glycerol-3-phosphate dehydrogenase.

Abstract: The invention relates to a process for the production of L-amino acids by fermentation using coryneform bacteria in which the mqo gene is enhanced.

Abstract: A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.

Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.

Abstract: A microorganism, which has an ability to produce L-amino acid, especially L-phenylalanine, L-tryptophane, L-tyrosine, L-threonine, or L-isoleucine, in which a phosphoenolpyruvate-producing ability is enhanced, is cultivated in a medium so that the L-amino acid is produced and accumulated in the medium to collect the L-amino acid.

Abstract: A bacterium which has an ability to produce an amino acid and in which a novel gene (rhtB) coding for a protein having an activity of making a bacterium having the protein L-homoserine-resistant is enhanced, is cultivated in a culture medium to produce and accumulate the amino acid in the medium, and the amino acid is recovered from the medium.

Abstract: E. coli strains derived form E. coli strain VNIIgenetika 472t23 and obtained by a process involving transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity and isolation of a transductant lacking threonine dehydrogenase activity are useful for producing L-threonine.

Abstract: A method of producing amino acids by culturing an amino acid auxotroph of a biologically pure strain of a type I methylotrophic bacterium of the genus Bacillus which exhibits sustained growth at 50° C. using methanol as a carbon and energy source and requiring vitamin B12 and biotin is provided.

Abstract: The present invention discloses the use, in the manufacture of a preventive and therapeutic drug of a brain disease, of a compound represented by formula (1): CH2═CH—CH2—S(O)n—R  (1) [wherein R represents a hydrogen atom, an alkyl group, an alkenyl group, a substituted alkyl group, a substituted alkenyl group, an alkylthio group, an alkenylthio group, a phenyl group, a substituted phenyl group, a heterocyclic group, or a group derived from an amino acid or an oligopeptide by deletion of one hydrogen atom, and which group may have a protective group; and n is 0, 1, or 2], a glycoside thereof, or a salt of the compound or the glycoside. The drug of the present invention for ameliorating brain diseases, inhibiting reduction of brain neurons and promoting branching of neurites, is useful for the prevention and treatment of brain diseases such as dementia in association with degeneration and sloughing of brain neurons.

Abstract: A recombinant DNA autonomously replicable in cells of coryneform bacteria, comprising a DNA sequence coding for an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA sequence coding for a diaminopimelate decarboxylase; a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, and comprising an enhanced DNA sequence coding for a diaminopimelate decarboxylase; and a method for producing L-lysine comprising the steps of cultivating the coryneform bacterium in an appropriate medium to allow L-lysine to be produced and accumulated in a culture of the bacterium, and collecting L-lysine from the culture.

Abstract: L-valine is produced by culturing a microorganism belonging to the genus Escherichia with the capability of producing L-valine or L-leucine wherein it requires lipoic acid for growth, a microorganism belonging to the genus Escherichia with the capability of producing L-valine or L-leucine wherein it is deficient in H+-ATPase activity, a microorganism belonging to the genus Escherichia wit the capability of producing L-valine or L-leucine wherein it requires lipoic acid for growth and is deficient in H+-ATPase activity, in the liquid medium to allow the L-valine to be produced and accumulated in a culture medium, and collecting it.

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with amplified lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are amplified and, in particular, overexpressed, and to a process for the preparation of L-lysine.

Abstract: The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.

Abstract: Processes for stereoselective enzymatic conversion of certain keto carboxylic acid derivatives to form the corresponding alkylamino acid compounds are described. The invention also concerns an engineered yeast host cell containing recombinant nucleic acid capable of expressing a phenylalanine dehydrogenase, as well as an engineered host cell containing recombinant nucleic acid capable of expressing a phenylalanine dehydrogenase enzyme and nucleic acid capable of expressing a formate dehydrogenase enzyme.

Abstract: The present invention provides a novel microbial strain and a method for effectively producing L-threonine. The novel strain can grow on a medium containing molasses, a much cheaper raw material than sucrose. The exemplary novel strain E. coli BKIIM B-5318 bears the plasmid pPRT614, which has threonine biosynthesis genes (thr A, B and C), the expression of which is regulated by a lambda-phage PR promoter and temperature-sensitive C1 repressor. The present microorganism is prototrophic with regard to isoleucine. The strain E. coli BKIIM B-5318 produces more than 70 g/l of L-threonine when cultured at 38-41.degree. C. for 32 hours in a medium containing molasses.

Abstract: 2-Aminopropane is used as the amine donor in the stereoselective synthesis of a chiral amine from a ketone with a transaminase. In a typical embodiment, (S)-1-methoxy-2-aminopropane is prepared by bringing methoxyacetone into contact with a transaminase in the presence of 2-aminopropane as an amine donor until a substantial amount of methoxyacetone is converted to (S)-1-methoxy-2-aminopropane and 2-aminopropane is converted to acetone. In a second embodiment, L-alanine is prepared by bringing pyruvic acid into contact with a transaminase in the presence of 2-aminopropane as an amine donor.

Abstract: A process is disclosed for the fermentative preparation of amino acids, in which an L-lysine producing bacterial strain of the species Corynebacterium glutamicum is cultivated in a nutrient medium and the amino acids can be isolated from the culture medium at the end of fermentation. After the vigorous growth phase, the bacterial culture has at its disposal a smaller quantity of assimilable carbon source than it could metabolize on the basis of the structure of the strain and the quanitity of other necessary supplements provided in the nutrient medium.

Abstract: A method of producing glutamic acid by culturing an amino acid auxotroph of a biologically pure strain of Bacillus methanolicus which exhibits sustained growth at 50.degree. C. using methanol as a carbon and energy source and requiring vitamin B.sub.12 and biotin is provided.

Abstract: A coryneform bacterium in which a DNA coding for a diaminopimelate decarboxylase and a DNA coding for a diaminopimelate dehydrogenase are enhanced is cultivated in a medium to allow L-lysine to be produced and accumulated in a culture, and L-lysine is collected from the culture.

Abstract: Disclosed is a process for preparing acylated amino esters and a process for preparing optically active amino esters from racemic amino esters with a carboxylic ester as acylating agent, whose acid component has a halogen, nitrogen, oxygen or sulfur atom neighboring the carbonyl carbon atom, in the presence of a hydrolase selected from the group of amidase, protease, esterase and lipase, and subsequent separation of the enantioselectively acylated amino ester from the non-acylated other enantiomer of the amino ester.

Abstract: A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture.

Abstract: The present invention relates to a method for controlling carbon source concentration in the aerobic cultivation of a microorganism. The substrate carbon source remains at a low level in a cultivation vessel during the culture feeding in aerobic fed-batch, continuous or cell-recycling continuous cultures. This is accomplished by monitoring the increase in pH or dissolved oxygen content in the culture medium and adding the feed solution intermittently into a cultivation vessel at a calculated feed rate using a feed control device controlled by a computer.The present invention further relates to a process for producing L-lysine by fermentation having the advantages over the prior methods, those being improved productivity, higher concentrations of accumulated product, and increased yields of L-lysine.

Abstract: A process produces an L-Lysine feed supplement with a final L-Lysine purity in the range theoretically between about 35% and 80%, measured as a percent of free-base per kg, and more preferably between about 50% and 80% L-Lysine. The process comprises adding a material containing L-Lysine to an L-Lysine fermentation broth or a fraction of an L-Lysine fermentation broth. The added material being an amount which brings a final L-Lysine feed supplement with an L-Lysine purity into a range theoretically between about 35% and 80%, measured as a percent of free-base per kg, and more preferably between about 50% and 80% L-Lysine. The fraction of L-Lysine fermentation broth is obtained by any suitable separating means such as ultrafiltration or centrifugation. The process also comprises a drying step which may involve any suitable drying means such as a spray granulator, spray dryer, tray dryer, drum dryer, rotary dryer, and tunnel dryer.

Abstract: Process for the efficient production of a substantially dust-free, free flowing granular L-Lysine in which the L-Lysine content is adjustable. Cells are removed from a fermentation broth containing L-Lysine to make a substantially cell free L-Lysine broth. Water is removed from the L-Lysine broth to form a concentrated L-Lysine broth. The L-Lysine content of the concentrated L-Lysine broth may be adjusted to be between about 35% and 76%, measured as a percent of freebase per kg, by adding material containing L-Lysine to provide an enriched L-Lysine broth. The enriched L-Lysine broth is agglomerated by means of an atomized spray of the enriched L-Lysine broth directed onto a fluidized bed of percolating L-Lysine particles. The L-Lysine particulates act as seeds for the agglomeration process wherein the L-Lysine particulates grow in size to provide a substantially dust-free, free flowing granular L-Lysine.

Abstract: A bacterium belonging to the genus Serratia, which is transformed by introducing into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia or Serratia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase originating from a bacterium belonging to the genus Escherichia or Serratia having mutation to desensitize feedback inhibition by L-lysine is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture.

Abstract: A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.

Abstract: L-tertiary-Leucine and L-phosphinothricine are obtainable by transamination of the corresponding keto acids as a precursor in the presence of amino acids as amino group donors. The reaction is preferably carried out with microorganisms or their transaminases.

Abstract: The present invention relates to novel strains of Escherichia coli and fermentation processes involving these microorganisms. More specifically, the present invention relates to genetically-modified Escherichia coli strains and the use thereof for the production of the amino acids, particularly members of the aspartate family of amino acids such as threonine. The present invention also relates to methods of preparing E. coli strains for use in the fermentative production of amino acids.

Abstract: A DNA encoding aspartokinase III derived from bacteria of the genus Escherichia and having mutation by which feedback inhibition with L-lysine is released is introduced into cells to form transformant bacteria of the genus Escherichia. These bacteria are incubated in an appropriate culture medium. An L-amino acid is produced and accumulated in the culture, and collected from this culture.

Abstract: The present invention provides a process for producing an L-amino acid which comprises culturing in a nutrient medium a microorganism which is capable of producing the L-amino acid and which can not grow in a synthetic medium containing said L-amino acid as the sole nitrogen source in an amount of 5 mg/ml or below, allowing the L-amino acid to accumulate in the culture, and recovering the L-amino acid from the culture.

Abstract: L-valine is produced by culturing a microorganism belonging to the genus Escherichia with the capability of producing L-valine or L-leucine wherein it requires lipoic acid for growth, a microorganism belonging to the genus Escherichia with the capability of producing L-valine or L-leucine wherein it is deficient in H.sup.+ -ATPase activity, a microorganism belonging to the genus Escherichia with the capability of producing L-valine or L-leucine wherein it requires lipoic acid for growth and is deficient in H.sup.+ -ATPase activity, in the liquid medium to allow the L-valine to be produced and accumulated in a culture medium, and collecting it.