Fat; Fatty Oil; Ester-type Wax; Higher Fatty Acid (i.e., Having At Least Seven Carbon Atoms In An Unbroken Chain Bound To A Carboxyl Group); Oxidized Oil Or Fat Patents (Class 435/134)
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Publication number: 20140234918Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: August 21, 2014Applicant: Novozymes A/SInventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Publication number: 20140234919Abstract: Methods and systems for producing lipids which may be used as biofuel are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production.Type: ApplicationFiled: July 6, 2012Publication date: August 21, 2014Applicant: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATIONInventors: Xiaochen Yu, Jijiao Zeng, Yubin Zheng, Mahesh Bule, Shulin Chen
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Patent number: 8809026Abstract: The present invention relates to processes for extracting lipid from vegetative plant parts such as leaves, stems, roots and tubers, and for producing industrial products such as hydrocarbon products from the lipids. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.Type: GrantFiled: March 15, 2013Date of Patent: August 19, 2014Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Thomas Vanhercke, James Robertson Petrie, Anna El Tahchy, Surinder Pal Singh, Qing Liu
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Patent number: 8809025Abstract: A method for culturing algae comprising, forming an emulsion comprising a gaseous stream and a media utilizing a high shear device, wherein the emulsion comprises gas bubbles, and wherein the high shear device comprises at least one toothed rotor and at least one stator; introducing the emulsion into a bioreactor; and introducing an algae into the bioreactor for growing the algae culture. Additionally, a method for producing liquids from an algae culture, the method comprising forming an emulsion comprising a buffer and algal components, wherein the emulsion comprises algal component globules; separating algal hydrocarbons; and processing algal hydrocarbons to form liquid hydrocarbons. Additionally, a system for producing liquids from an algae culture comprising at least one high shear device.Type: GrantFiled: October 6, 2010Date of Patent: August 19, 2014Assignee: H R D CorporationInventors: Abbas Hassan, Aziz Hassan, Rayford G. Anthony, Gregory G. Borsinger
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Publication number: 20140228586Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.Type: ApplicationFiled: July 5, 2012Publication date: August 14, 2014Applicant: Verdezyne, Inc.Inventors: Tom Beardslee, Stephen Picataggio, E. Dudley Eirich, Jose Miguel LaPlaza
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Publication number: 20140228587Abstract: The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production.Type: ApplicationFiled: July 5, 2012Publication date: August 14, 2014Inventors: Tom Beardslee, Stephen Picataggio, Alex Hutagalung, Tom Fahland
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Publication number: 20140227748Abstract: Novel strains of microalgae belonging to the Monodus genus allow optimum production of polyunsaturated fatty acids, notably EPA, in mixotrophic mode. A method for selecting and culturing such strains, using a discontinuous supply of light in the form of flashes is also described.Type: ApplicationFiled: June 7, 2012Publication date: August 14, 2014Applicant: FERMENTALGInventors: Pierre Calleja, Khadidja Romari
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Patent number: 8802924Abstract: An isolated protein which is at least partially encoded by a polynucleotide sequence encoding a novel elongase is provided together with a composition which includes the isolated protein. A transgenic plant or a transgenic seed transformed by a polynucleotide encoding a protein which is at least partially encoded by a novel elongase is also provided. The invention also includes a process for making a very long-chain polyunsaturated fatty acid in a transformed cell or plant expressing the isolated protein which is at least partially encoded by a polynucleotide sequence encoding a novel elongase.Type: GrantFiled: November 26, 2009Date of Patent: August 12, 2014Assignee: Ben Gurion University of the Negev Research and Development AuthorityInventors: Zvi Hacohen, Inna Khozin Goldberg, Rivka Ofir, Iskandarov Umidjon
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Publication number: 20140220646Abstract: An apparatus for treating food waste and extracting bio oil includes: a fermentation dryer which ferments and decomposes food waste for 24 hours to reduce moisture contained in the food waste to 40 to 50%, a crusher which crushes the food waste into small particles, a distillation tank which heats the crushed food waste at low pressure and temperature so that oil is gasified by means of low temperature thermal decomposition to form oil gas, a distillation tower in which the oil gas is cooled to be liquefied and separated into moisture (carbon liquid fertilizer) and oil, a vacuum storage tank which collects the separated oil, and a centrifugal separator which rectifies the oil. The apparatus extracts a carbon liquid fertilizer, bio oils, and activated carbon while treating organic substances such as food waste and residual product compost.Type: ApplicationFiled: April 26, 2012Publication date: August 7, 2014Applicant: G&P BIOTECH CO., LTD.Inventor: Won Im Lim
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Publication number: 20140220645Abstract: Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: ApplicationFiled: December 18, 2013Publication date: August 7, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Quinn Qun Zhu, Seung-Pyo Hong, Dongming Xie, Zhixiong Xue, Hyeryoung Yoon, Michael Dauner
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Publication number: 20140221207Abstract: A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, algae, arachnids, insects, fungi, weeds and nematodes) as well as methods for controlling algae using said species of Burkholderia. Also provided are natural products derived from a culture of said species and methods of controlling algae and/or arachnids using said natural products.Type: ApplicationFiled: August 14, 2012Publication date: August 7, 2014Applicant: MARRONE BIO INNOVATIONS, INC.Inventors: Ratnakar Asolkar, Marja Koivunen, Pamela Marrone
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Publication number: 20140215904Abstract: Disclosed are fatty ester compositions comprising beta-hydroxy fatty esters, as well as methods for producing beta-hydroxy fatty esters, and recombinant microorganisms useful in methods of producing beta-hydroxy fatty estersType: ApplicationFiled: March 30, 2012Publication date: August 7, 2014Applicant: LS9, Inc.Inventors: Archana Pandey, Mathew A Rude, Fernando A Sanchez-Riera, Bernardo M. da Costa
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Publication number: 20140220638Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher Pmax per unit chlorophyll with little to no reduction in Pmax per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.Type: ApplicationFiled: December 6, 2013Publication date: August 7, 2014Applicant: Synthetic Genomics, Inc.Inventors: Shaun Bailey, Jay McCarren, Soyan Leung Lieberman, Jonathan E. Meuser, Anna E. Romano, Daniel Yee, Leah Soriaga, Robert C. Brown, Joseph C. Weissman, Roger C. Prince, Robert D. Nielsen, Ariel S. Schwartz
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Publication number: 20140212936Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.Type: ApplicationFiled: April 3, 2014Publication date: July 31, 2014Applicant: DSM IP Assets B.V.Inventors: Craig M. Ruecker, Swithwin Patrick Adu-peasah, Brian S. Engelhardt, George T Veeder, III
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Publication number: 20140212935Abstract: The present invention relates to an engineered bacteria for producing short chain fatty acid with the overexpression of a long chain (>C12) acyl-ACP thioesterases (long-TE) and a short chain (?C12) acyl-ACP thioesterases (short-TE).Type: ApplicationFiled: January 22, 2014Publication date: July 31, 2014Applicant: William Marsh Rice UniversityInventors: Ka-Yiu SAN, Songi HAN
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Publication number: 20140215654Abstract: The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.Type: ApplicationFiled: March 12, 2013Publication date: July 31, 2014Inventor: David Davis
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Patent number: 8790914Abstract: The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms that manufacture non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils. Also provided are compositions comprising depolymerized cellulosic materials and oil-bearing microbes. Some methods of microbial fermentation are provided that comprise combining depolymerized cellulosic materials with other non-cellulosic feedstocks to enhance the economics of renewable fuel manufacturing. Particular advantages of the processes provided herein include production of oils rather than alcohols through cellulosic processes. Additional advantages include methods for manufacturing high nutrition oils from non-edible feedstocks such as wood chips, switchgrass, and bagasse.Type: GrantFiled: June 2, 2008Date of Patent: July 29, 2014Assignee: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 8790901Abstract: Methods of producing an unsaturated free fatty acid comprising at least 18 carbon atoms are provided. In some embodiments, the methods comprise culturing an engineered microorganism in a culture medium, wherein the engineered microorganism comprises at least one recombinant enzyme selected from acyl-lipid desaturase delta-9 (EC:1.14.19.1), acyl-lipid desaturase delta-12 (EC:1.14.19.6), acyl-lipid desaturase delta-15 (EC:1.14.19.-), and thioesterase (EC:3.1.2.14). Engineered microorganisms comprising at least one of those recombinant enzymes are also provided. The methods and organisms can be used to produce at least one free fatty acid selected from oleic acid, linoleic acid and ?-linolenic acid.Type: GrantFiled: December 14, 2011Date of Patent: July 29, 2014Assignee: Pronutria, Inc.Inventors: Subhayu Basu, Gaozhong Shen, Brett Boghigian, David Young, David Berry, Geoffrey von Maltzahn
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Patent number: 8785163Abstract: The present invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruberi, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention.Type: GrantFiled: July 30, 2008Date of Patent: July 22, 2014Assignee: BASF Plant Science GmbHInventors: Toralf Senger, Jörg Bauer
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Patent number: 8785164Abstract: Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.Type: GrantFiled: May 23, 2012Date of Patent: July 22, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Seung-Pyo Hong, Quinn Qun Zhu
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Patent number: 8785727Abstract: The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: GrantFiled: April 29, 2009Date of Patent: July 22, 2014Assignee: Rothamsted Research Ltd.Inventors: Joerg Bauer, Johnathan A. Napier, Olga Sayanova
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Publication number: 20140199739Abstract: Novel strains of microalgae belonging to the Isochrysis genus, allowing optimum production of polyunsaturated fatty acids, notably EPA, in mixotrophic mode, and a method for selecting and culturing such strains, using a discontinuous supply of light in the form of flashes are described.Type: ApplicationFiled: June 20, 2012Publication date: July 17, 2014Applicant: FERMENTALGInventors: Pierre Calleja, Khadidja Romari
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Publication number: 20140199738Abstract: A method of preparing a volatile fatty acids (VFAs) is provided. More particularly, the method includes chemically or biologically pretreating a residue of algae to obtain an extract of the algae residue, filtering the extract of the algae residue and anaerobically fermenting the filtrate. The method of preparing VFAs can be used regardless of the kinds of algae, a process of producing VFAs can be simplified since there is no need to develop an enzyme for anaerobic fermentation, and a yield of VFAs can be increased since the VFAs can be produced from different kinds of organic components such as proteins or fats in addition to carbohydrates. Accordingly, the method can be useful in producing VFAs in an economical and efficient manner.Type: ApplicationFiled: December 16, 2011Publication date: July 17, 2014Applicant: Pukyong National University Industry- University Cooperation FoundationInventors: Hee-Chul Woo, Ho-Nam Chang, Yeong-Joong Jeon, Dong-Jin Suh, Byung-Soo Chun, Kyeong-Keun Oh, Kyoung-Heon Kim, Du-Woon Kim, Jae-Hyung Choi
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Publication number: 20140199740Abstract: The present disclosure comprises methods, apparatus, components, and techniques for pretreatment of biomaterials using targeted wet oxidation. The targeted wet oxidation pretreatment is an upstream method for converting solid biomass into fuels and/or specialty chemicals. Embodiments of the present disclosure comprise methods carried out on biomaterials to selectively oxidize lignin components of the biomass, thereby resulting in bio accessible/digestible biomass fibers. In embodiments of the present disclosure, such methods may comprise pretreatment processes to prepare the biomaterials for a subsequent fermentation or other like conversion may be carried out to result in useful bio fuels or other bioproducts.Type: ApplicationFiled: January 16, 2014Publication date: July 17, 2014Applicant: CLEAN-VANTAGE LLCInventors: RICHARD ALAN MERRILL, Kenn Petersen
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Patent number: 8778643Abstract: The present disclosure relates to methods of increasing lipid levels in an algal or yeast cell population, methods of producing saturated or monounsaturated triacylglycerols in an algal or yeast cell population, and methods of decreasing polyunsaturated triacylglycerol production in an algal or yeast cell population by contacting the cell population with a chemical compound that is capable of increasing lipid levels or altering the lipid composition in the cell population.Type: GrantFiled: March 13, 2013Date of Patent: July 15, 2014Assignee: The Regents of the University of CaliforniaInventors: Annaliese Kirsten Franz, Megan Danielewicz, Diana Meiying Wong, Lisa Adele Anderson
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Patent number: 8778644Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.Type: GrantFiled: September 16, 2013Date of Patent: July 15, 2014Assignee: Commonwealth Scientific and Industrial Research OrganisationInventors: Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
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Publication number: 20140193866Abstract: The invention provides novel methods for making or modifying oils, e.g., plant animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof.Type: ApplicationFiled: May 24, 2013Publication date: July 10, 2014Applicant: DSM IP ASSETS B.V.Inventor: DSM IP Assets B.V.
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Publication number: 20140193867Abstract: Engineered bacteria that produce a predominance of odd-chain free fatty acids.Type: ApplicationFiled: December 12, 2013Publication date: July 10, 2014Inventors: Ka-Yiu SAN, Hui WU
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Publication number: 20140194513Abstract: The present invention relates to a fermented vegetable oil, to a method for preparing the oil, and to a composition including the oil. More particularly, the present invention relates to a technique for providing a fermented vegetable oil having the effects of enhancing emulsion stability due to water retention ability, improving texture and flavor, and enhancing moisturization.Type: ApplicationFiled: August 24, 2012Publication date: July 10, 2014Applicant: DAMY CHEMICAL CO., LTD.Inventors: Kwang-Nyeon Kim, Ju-Hyun Son, Hee-Sik Kim, Jong-Seok Yun
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Publication number: 20140196177Abstract: An isolated protein which is at least partially encoded by a polynucleotide sequence encoding a novel acyl-CoA: diacylglycerol acyltransferase 1—like gene (PtDGAT1) from the diatom Phaeodactylum tricomutum is provided together with a composition which includes the isolated protein and a transgenic organism transformed by a polynucleotide encoding same. The invention also provides a method for producing or enhancing the production of oil or triacylglycerols with high saturated fatty acids content.Type: ApplicationFiled: November 3, 2011Publication date: July 10, 2014Applicant: Ben Gurion University of the Negev Research and Development AuthorityInventors: Freddy Guiheneuf, Stefan Leu, Aliza Zarka, Inna Khozin-Goldberg, Sammy Boussiba
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Patent number: 8772575Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.Type: GrantFiled: September 26, 2012Date of Patent: July 8, 2014Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Publication number: 20140186908Abstract: Disclosed are processing systems and processes for carrying out enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food and detergent industries.Type: ApplicationFiled: December 30, 2013Publication date: July 3, 2014Applicant: Trans Biodiesel Ltd.Inventors: Sobhi BASHEER, Usama MOHSEN
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Publication number: 20140186909Abstract: The present invention provides a photobioreactor for a phototrophic microorganism, and culture medium therefor, comprising a capsule, the capsule comprising an elongated body comprised of a first, flexible polymer film that is at least partially transparent to light of a wavelength that is photosynthetically active in a phototrophic microorganism and divided width wise into a plurality of adjacent channels, each channel having a major cross-sectional dimension that is not more than about one-third of the length of the elongated body, when the elongated body is inflated; and a fluid distribution structure coupled to the elongated body adapted for fluid communication with the plurality of adjacent channels that distributes a flow of culture medium amongst the plurality of channels, wherein the fluid distribution structure is comprised of a second, flexible polymer film.Type: ApplicationFiled: August 1, 2012Publication date: July 3, 2014Inventors: Kevin J. Calzia, Max Brett Tuttman, John E. Longan, Thomas A. Urbanik
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Publication number: 20140186907Abstract: The present invention generally relates to oil compositions and methods of producing such oil compositions. More particularly, the present invention relates to an oil composition recovered from a fermentation product as well as methods of recovering such oil compositions for use in various processes such as bio-diesel production as well as in various edible applications.Type: ApplicationFiled: December 23, 2013Publication date: July 3, 2014Applicant: Poet Research, Inc.Inventor: Jason Bootsma
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Publication number: 20140186905Abstract: Subject matter of the invention is a biotechnological process for the production of organic compounds with the aid of at least one alkL gene product.Type: ApplicationFiled: August 15, 2012Publication date: July 3, 2014Applicant: Evonik Degussa GmbHInventors: Steffen Schaffer, Jasmin Gielen, Nicole Decker, Nicole Kirchner, Thomas Haas, Markus Poetter, Harald Haeger
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Publication number: 20140186906Abstract: Recombinant microbial cells are disclosed herein that produce an oil comprising at least 28 percent eicosapentaenoic acid (EPA) measured as a weight percent of dry cell weight. These cells may comprise a polynucleotide sequence encoding an active acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) comprising at least one amino acid mutation in a membrane-bound O-acyltransferase motif. In addition, the cells may comprise a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and/or one or more polynucleotides encoding phospholipid:diacylglycerol acyltransferase (PDAT), delta-12 desaturase, a dihomo-gamma-linolenic acid (DGLA) synthase multizyme, delta-8 desaturase, malonyl-CoA synthetase (MCS), or acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT). Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.Type: ApplicationFiled: December 18, 2013Publication date: July 3, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Quinn Qun Zhu, Seung-Pyo Hong, Pamela L. Sharpe, Narendra S. Yadav, Xiaochun Fan, Michael Dauner
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Patent number: 8765424Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.Type: GrantFiled: July 6, 2012Date of Patent: July 1, 2014Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
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Patent number: 8765423Abstract: It is an object to provide a novel diacylglycerol acyltransferase. The present invention relates to a diacylglycerol acyltransferase, a polynucleotide encoding the same, and so on. The present invention provides a polynucleotide comprising the nucleotide sequence of, e.g., SEQ ID NO: 1 or 4, a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2, an expression vector and transformant comprising the polynucleotide, a method for producing a lipid or fatty acid composition using the transformant, or a food, etc. comprising the lipid or fatty acid produced by the method.Type: GrantFiled: December 20, 2010Date of Patent: July 1, 2014Assignee: Suntory Holdings LimitedInventor: Misa Ochiai
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Patent number: 8765422Abstract: Disclosed are methods and compositions related to ONC-T18, D4-desaturases, D5 elongases, their isolation, characterization, production, identification, and use for fatty acid production, as well as organisms containing these compositions and organisms expressing them.Type: GrantFiled: October 31, 2007Date of Patent: July 1, 2014Assignee: DSM Nutritional Products AGInventors: Adam Burja, Gabrielle S. Chafe, Helia Radianingtyas
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Publication number: 20140179940Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.Type: ApplicationFiled: April 2, 2012Publication date: June 26, 2014Inventors: Derek L. Greenfield, Louis G. Hom, Fernando A. Sanchez-Riera, Zhihao Hu, Vikranth Arlagadda, Eli S. Groban, Scott A. Frykman
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Publication number: 20140178951Abstract: A process for producing products from biomass comprising fermenting biomass to produce a first product stream comprising carboxylic acid salts; acidifying at least one of the first product stream and a second product stream to produce a third product stream comprising acids; extracting such acids from the third product stream with a solvent; separating the extracting solvent from the acids to produce the separated extracting solvent and a fourth product stream comprising acids; and processing the fourth product stream to produce a fifth product stream.Type: ApplicationFiled: December 19, 2013Publication date: June 26, 2014Applicant: EE-TERRABON BIOFUELS, LLCInventors: Michael Kyle ROSS, Cesar B. Granda
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Publication number: 20140178950Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.Type: ApplicationFiled: December 6, 2013Publication date: June 26, 2014Applicant: Solazyme, Inc.Inventors: Scott Franklin, Shane Brubaker, George N. Rudenko, Riyaz Bhat, Matthew Shoa-Azar, Aravind Somanchi, Aren Ewing
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Publication number: 20140179942Abstract: The invention relates to a micro-organism comprising a hydrogenase enzyme system which is capable of converting carbon dioxide into formic acid and a second enzyme system which is capable of converting formic acid into aliphatic carboxylic acids having a chain length of five or more carbon atoms. Also described are various methods for producing oil, as well as other aspects of the invention.Type: ApplicationFiled: July 13, 2012Publication date: June 26, 2014Applicant: VERDANT BIOPRODUCTS LIMITEDInventor: Irene Finnegan
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Publication number: 20140170715Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 10, 2012Publication date: June 19, 2014Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Publication number: 20140171608Abstract: In certain aspects, the disclosure provides methods for producing polymers from alkenone-producing algae, such as algae species of the Isochrysis family.Type: ApplicationFiled: February 24, 2014Publication date: June 19, 2014Applicants: Marine Biological Laboratory, WOODS HOLE OCEANOGRAPHIC INSTITUTIONInventors: Scott R. Lindell, Christopher M. Reddy
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Publication number: 20140170716Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.Type: ApplicationFiled: February 19, 2014Publication date: June 19, 2014Applicant: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 8753852Abstract: The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.Type: GrantFiled: January 12, 2012Date of Patent: June 17, 2014Assignee: Danisco US Inc.Inventors: Brian E. Jones, William D. Grant, Shaun Heaphy, Susan Grant, Helen Rees
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Patent number: 8753851Abstract: Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae.Type: GrantFiled: April 16, 2010Date of Patent: June 17, 2014Assignee: LiveFuels, Inc.Inventors: David Stephen, Gaye Elizabeth Morgenthaler, Benjamin Chiau-pin Wu, David Vancott Jones
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Publication number: 20140162329Abstract: The present invention provides novel diacylglycerol acyltransferase (DGAT) genes comprising Pleckstrin Homology (PH) domains. The present invention also provides for recombinant cells, such as algae, transformed with acyltransferase genes, such as DGAT, comprising PH domains, and methods of using such recombinant cells to produce increased triglyceride levels.Type: ApplicationFiled: December 6, 2012Publication date: June 12, 2014Applicant: ExxonMobil Research and Engineering CompanyInventors: Jennifer Coppersmith, Rekha Seshadri, Toby Howard Richardson
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Publication number: 20140162328Abstract: A process for increasing the production of a glycolytic intermediate and/or an organic compound as defined herein by a cell that is able to express a nucleic acid molecule, wherein the expression of the nucleic acid molecule gives the cell the ability to increase the production of a glycolytic intermediate and/or the organic compound and wherein the carbon substrate is sugar and/or protein.Type: ApplicationFiled: December 12, 2012Publication date: June 12, 2014Inventor: Trent Nguyen