Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
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Patent number: 8415128Abstract: Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.Type: GrantFiled: July 26, 2011Date of Patent: April 9, 2013Assignee: Pacific Biosciences of California, Inc.Inventors: John Eid, Devon Murphy, Geoffrey Otto, Stephen Turner
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Patent number: 8409808Abstract: The invention provides methods for identifying conditions of low grade cervical dysplasia and assessing the progressive potential of individual lesions to develop into high grade cervical dysplasia and cervical squamous cell cancer as well as cervical adenocarcinoma.Type: GrantFiled: February 21, 2006Date of Patent: April 2, 2013Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Thomas Ried, Kerstin Heselmeyer-Haddad, Winfried Steinberg, Gert Auer, Sonia Andersson, Catharina Larsson
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Patent number: 8409583Abstract: CgtB proteins with enhanced beta 1,3-galactosaminyltransferase activity, nucleic acids that encode the CgtB proteins and methods for use of the CgtB proteins.Type: GrantFiled: April 18, 2008Date of Patent: April 2, 2013Assignee: National Research Council of CanadaInventors: Stephane Bernatchez, Michel Gilbert, Warren Wakarchuk
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Publication number: 20130081152Abstract: The invention relates to a nucleic acid molecule that improves aroma production in a plant, and a cell and a transgenic plant comprising the nucleic acid molecule. A protein and a protein complex for catalyzing the synthesis of a monoterpene and a precursor thereof and uses for improving the production of aroma in a plant are also provided.Type: ApplicationFiled: September 22, 2011Publication date: March 28, 2013Applicant: NATIONAL CHENG KUNG UNIVERSITYInventors: HONG-HWA CHEN, YU-YUN HSIAO, WEN-CHIEH TSAI, WEN-HUEI CHEN
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Publication number: 20130078267Abstract: The invention herein disclosed is related to epitopes useful in methods of diagnosing, treating, and preventing coeliac disease. Therapeutic compositions which comprise at least one epitope are provided.Type: ApplicationFiled: July 5, 2012Publication date: March 28, 2013Inventors: Robert Anderson, Tim Beissbath, Jason Tye-Din
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Publication number: 20130078239Abstract: The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DGAT2 family member gene has been introduced or disrupted. The invention still further provides isolated DGAT2 family member proteins, fusion proteins, antigenic peptides and anti-DGAT2 family member antibodies. Methods of use of the provided DGAT2 family member compositions for screening, diagnostic and therapeutic methods in connection with obesity disorders are also disclosed.Type: ApplicationFiled: November 6, 2012Publication date: March 28, 2013Applicant: MILLENNIUM PHARMACEUTICALS, INC.Inventor: Millennium Pharmaceuticals, Inc.
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Patent number: 8399738Abstract: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.Type: GrantFiled: March 30, 2012Date of Patent: March 19, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Johan M. Stoop, Stephen M. Allen, Perry G. Caimi
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Publication number: 20130065293Abstract: The present invention has objects to provide a glucan useful as water-soluble dietary fiber, its preparation and uses. The present invention solves the above objects by providing a branched ?-glucan, which is constructed by glucose molecules and characterized by methylation analysis as follows: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol is 0.5% or higher in the partially methylated glucitol acetates; a novel ?-glucosyltransferase which forms the branched ?-glucan, processes for producing them, and their uses.Type: ApplicationFiled: November 1, 2012Publication date: March 14, 2013Applicant: Hayashibara Co., Ltd.Inventor: Hayashibara Co., Ltd.
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Publication number: 20130059034Abstract: The present invention relates to polypeptides, specifically polypeptides having transgalactosylating activity and nucleic acids encoding these, and their uses in e.g. dairy product.Type: ApplicationFiled: March 29, 2011Publication date: March 7, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Morten Krog Larsen, Charlotte Horsmans Poulsen
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Publication number: 20130059331Abstract: The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.Type: ApplicationFiled: April 19, 2011Publication date: March 7, 2013Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Wilbert Herman Marie Heijne
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Patent number: 8389252Abstract: Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol.Type: GrantFiled: May 5, 2009Date of Patent: March 5, 2013Assignee: Butamax(TM) Advanced Biofuels LLCInventor: Robert A. LaRossa
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Publication number: 20130052178Abstract: The present invention relates to a method for reducing cholesterol absorption in an animal comprising administering to the animal a composition comprising an effective amount of at least one cholesterol ester.Type: ApplicationFiled: May 6, 2011Publication date: February 28, 2013Applicant: DuPont Nutrition Biosciences ApSInventors: Maria Jadwiga Bauerek, Charlotte Horsmans Poulsen, Jørn Borch Søe, Bent Raungaard, Leif Schauser
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Patent number: 8383383Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: August 30, 2012Date of Patent: February 26, 2013Assignees: Novozymes A/S, Novozymes, Inc.Inventors: Debbie Yaver, Suzanne Otani
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Patent number: 8383382Abstract: The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.Type: GrantFiled: September 24, 2010Date of Patent: February 26, 2013Assignee: Codexis, Inc.Inventors: Lynne Gilson, Steven James Collier, Joly Sukumaran, Wan Lin Yeo, Oscar Alvizo, Ee Ling Teo, Robert John Wilson, Junye Xu
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Patent number: 8383374Abstract: The subject invention provides materials and methods wherein unique and advantageous combinations of gene mutations are used to direct carbon flow from sugars to a single product. The techniques of the subject invention can be used to obtain products from native pathways as well as from recombinant pathways. In preferred embodiments, the subject invention provides new materials and methods for the efficient production of acetate and pyruvic acid.Type: GrantFiled: June 7, 2011Date of Patent: February 26, 2013Assignee: University of Florida Research Foundation, Inc.Inventors: Thomas B. Causey, Lonnie O'Neal Ingram, Keelnatham Shanmugam, Shengde Zhou
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Publication number: 20130045506Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 13, 2012Publication date: February 21, 2013Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
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Patent number: 8377673Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-9 elongases in plants.Type: GrantFiled: January 11, 2012Date of Patent: February 19, 2013Assignee: E.I. du Pont de Nemours and CompanyInventor: Howard G. Damude
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Publication number: 20130039894Abstract: The present invention relates to polypeptides transiently activating Ras homolog gene family member A (RhoA) GTPase, polynucleotides encoding said polypeptides and pharmaceutical compositions comprising said polypeptides or said polynucleotides. The present invention further relates to the use of said polypeptides, said polynucleotides or said pharmaceutical compositions for long-term treatment of damage of the peripheral or central nervous system.Type: ApplicationFiled: March 31, 2010Publication date: February 14, 2013Inventors: Gudrun Ahnert-Hilger, Gisela Grosse, Fred Hofmann, Ingo Just, Markus Höltje, Stefanie Hülsenbeck
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Publication number: 20130040348Abstract: The present invention provides an enzyme having an activity of transferring a methyl group to lignans (a lignan methylation activity) (e.g., a protein comprising the amino acid sequence of SEQ ID NO:2 or variants thereof); a gene encoding the enzyme (e.g., a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1 or variants thereof); a method for producing methylated lignans using the gene; and so on.Type: ApplicationFiled: May 13, 2010Publication date: February 14, 2013Applicant: Suntory Holdings LimitedInventors: Eiichiro Ono, Toshiaki Umezawa, Takefumi Hattori, Shiro Suzuki
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Publication number: 20130040297Abstract: The invention relates to a process for the production of cells which are capable of converting arabinose, comprising the following steps: a) Introducing into a host strain that cannot convert arabinose, the genes AraA, araB and araD, this cell is designated as constructed cell; b) Subjecting the constructed cell to adaptive evolution until a cell that converts arabinose is obtained, c) Optionally, subjecting the first arabinose converting cell to adaptive evolution to improve the arabinose conversion; the cell produced in step b) or c) is designated as first arabinose converting cell; d) Analysing the full genome or part of the genome of the first arabinose converting cell and that of the constructed cell; e) Identifying single nucleotide polymorphisms (SNP's) in the first arabinose converting cell; and f) Using the information of the SNP's in rational design of a cell capable of converting arabinose; g) Construction of the cell capable of converting arabinose designed in step f).Type: ApplicationFiled: April 19, 2011Publication date: February 14, 2013Applicant: DSM IP ASSETSInventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Wilbert Herman Marie Heijne, Gijsberdina Pieternella Van Suylekom
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Patent number: 8372989Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: November 3, 2004Date of Patent: February 12, 2013Assignee: Cargill, IncorporatedInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, John Rosazza, Lishan Zhao, David P. Weiner
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Patent number: 8372617Abstract: The present invention provides an extremely useful and novel ?-galactoside-?2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.Type: GrantFiled: April 25, 2012Date of Patent: February 12, 2013Assignee: Japan Tobacco Inc.Inventors: Takeshi Yamamoto, Yoshimitsu Takakura, Toshiki Mine, Yoko Hamada, Hitomi Kajiwara, Masako Ichikawa, Hiroshi Tsukamoto
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Patent number: 8372616Abstract: It is a subject of this invention to isolate a gene group of enzymes involved in the biosynthesis of vitamin E of Para rubber tree, and to determine the base sequence of each gene. According to this invention, genes encoding enzymes involved in the vitamin E biosynthesis were isolated from Para rubber tree and the base sequences of these genes were determined. Since vitamin E is an antioxidant existing in nature, it is expected that transformation of a plant by using the genes obtained in the invention would result in an increase in the vitamin E content of the plant and, in its turn, contribute to the prevention of rubber from aging.Type: GrantFiled: March 19, 2008Date of Patent: February 12, 2013Assignees: Bridgestone Corporation, Badan Pengkajian Dan Penerapan TeknologiInventors: Tomoki Sando, Norie Watanabe, Koichiro Gyokusen, Eiichiro Fukuzaki, Akio Kobayashi, Haska Nadirman, Purbowasito Wahyu
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Publication number: 20130034627Abstract: The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.Type: ApplicationFiled: April 11, 2012Publication date: February 7, 2013Inventors: Janne Brunstedt, Jens Frisbæk Sorensen, Jørn Borch Søe, Charlotte Johansen Vedel, Birgitte Ø. Wittschieben, Jørn Dalgaard Mikkelsen, Charlotte Horsmans Poulsen, Lene B. Jensen, Lone B. Miller, Morten K. Larsen, Rikke H. Lorentzen, Charlotte R. Thoudahl, Marguerite A. Cervin, Richard R. Bott
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Publication number: 20130035403Abstract: The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids.Type: ApplicationFiled: October 19, 2010Publication date: February 7, 2013Applicant: Evonik Degussa GmbHInventors: Steffen Schaffer, Mirja Wessel, Anja Thiessenhusen
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Patent number: 8367395Abstract: The present invention provides methods for production of one or more sterol compounds. Further provided are methods and systems for producing engineered oleaginous yeast or fungi that are capable of production of one or more sterol compounds, and compositions which utilize the produced sterol compound(s).Type: GrantFiled: September 28, 2007Date of Patent: February 5, 2013Assignee: DSM IP Assets B.V.Inventors: Richard B. Bailey, Joshua Trueheart, Kevin T. Madden
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Patent number: 8367388Abstract: Improved compositions comprising a cross-linkable protein or polypeptide, and a non-toxic material which induces cross-linking of the cross-linkable protein. The compositions are optionally and preferably prepared in a non-phosphate buffer solvent. Optionally and preferably, the cross-linkable protein includes gelatin and any gelatin variant or variant protein as described herein. Optionally and preferably, the non-toxic material comprises transglutaminase (TG), which may optionally comprise any type of calcium dependent or independent transglutaminase, which may for example optionally be a microbial transglutaminase (mTG).Type: GrantFiled: June 18, 2009Date of Patent: February 5, 2013Assignee: Lifebond Ltd.Inventors: Orahn Preiss Bloom, Ishay Attar, Natalie Iram, Chagai Komlos, Meir Haber
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Publication number: 20130029384Abstract: The present invention relates to a sucrose phosphorylase from Bifidobacterium adolescentis which is useful as a biocatalyst in carbohydrate conversions at high temperatures. Indeed, the biocatalysts of the present invention are enzymatically active for a time period of at least 16 h and up to 1 to 2 week(s) at a temperature of at least 60° C. The biocatalysts of the present invention are: a) immobilized on an enzyme carrier, or b) are part of a cross-linked enzyme aggregate (CLEA), and/or c) are mutated, and/or d) are enzymatically active in the continuous presence of their substrate.Type: ApplicationFiled: April 1, 2011Publication date: January 31, 2013Applicant: UNIVERSITEIT GENTInventors: An Cerdobbel, Tom Desmet, Wim Soetaert
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Publication number: 20130029400Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: September 6, 2012Publication date: January 31, 2013Applicant: BATTELLE ENERGY ALLIANCE, LLCInventors: Brady D. Lee, Deborah T. Newby, Jeffrey A. Lacey, David N. Thompson, Vicki S. Thompson, William A. Apel, Francisco F. Roberto, David W. Reed
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Publication number: 20130029908Abstract: The invention relates to Cerberus/Dan/Gremlin polypeptides or variants thereof for use in treating a variety of disorders associated with myostatin, nodal and GDF-11. Preferred polypeptides are Coco or Cerberus derivatives.Type: ApplicationFiled: June 26, 2012Publication date: January 31, 2013Applicant: Acceleron Pharma, Inc.Inventors: John Knopf, Jasbir Seehra
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Patent number: 8361771Abstract: The present invention relates to methods of improving the introduction of DNA into bacterial host cells.Type: GrantFiled: July 10, 2012Date of Patent: January 29, 2013Assignee: Novozymes, Inc.Inventors: Michael Thomas, Michael Rey
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Patent number: 8361770Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid sulfotyrosine and translation systems.Type: GrantFiled: December 14, 2011Date of Patent: January 29, 2013Assignee: The Scripps Research InstituteInventors: Chang C. Liu, Peter G. Schultz
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Publication number: 20130023016Abstract: The present invention relates to a polynucleotide that is active to an acetyl glutamate synthase and acetyl ornithinase which are associated with ornithine or arginine biosynthesis from Corynebacterium glutamicum. The present invention also relates to a polypeptide encoded by said polynucleotide, a recombinant vector comprising said polynucleotide, to a transformant obtained by introducing said recombinant vector to a host microorganism for producing L-ornithine or L-arginine, and transforming the recombinant vector, and to a method for producing L-ornithine or L-arginine by culturing said transformant. The activity of the transformant of the present invention to an acetyl glutamate synthase and acetyl ornithinase is increased as compared to an intrinsic activity, and thus L-ornithine or L-arginine can be produced, at a high yield rate, from the transformant of the present invention.Type: ApplicationFiled: December 29, 2010Publication date: January 24, 2013Inventors: Jin-Man Cho, Hye-Won Kim, Ji-Hye Lee, Jae-Yong Cho
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Publication number: 20130023416Abstract: Mutant rice resistant/tolerant to ACCase inhibiting herbicides, in particular FOP herbicides, are listed in Table 1. The ACCase inhibitng herbicides used for selection include quizalofop. An exemplary mutant rice tolerant to an ACCase herbicide is disclosed, with a rice genome having G2096S or the equivalent, in the carboxyl transferase domain of the ACCase coding gene, using the Black-Grass numbering system. This mutation shows differential response to FOPs vs. DIMs herbicides, and a greater differential with comparable non-resistant rice lines. Methods to control weeds and methods to produce herbicide resistant rice including transgenic rice, are disclosed.Type: ApplicationFiled: July 20, 2012Publication date: January 24, 2013Applicant: RICETEC AKTIENGESELLSCHAFTInventors: Melissa Hinga, Steven Griffin, Melissa Shannon Moon, Russell D. Rasmussen, Federico Cuevas
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Patent number: 8357834Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.Type: GrantFiled: August 18, 2011Date of Patent: January 22, 2013Assignee: Monsanto Technology LLCInventors: Brian Martinell, Michael Petersen, David Somers, Yuechun Wan, Edward Williams, Xudong Ye
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Publication number: 20130017204Abstract: The invention relates to the polynucleotide sequence of a nontypeable stain of Haemophilus influenzae (NTHi) and polypeptides encoded by the polynucleotides and uses thereof. The invention also relates to NTHi genes which are upregulated during or in response to NTHi infection of the middle ear and/or the nasopharynx.Type: ApplicationFiled: July 19, 2012Publication date: January 17, 2013Applicants: THE BOARD OF REGENTS OF UNIVERSITY OF OKLAHOMA, NATIONWIDE CHILDREN'S HOSPITAL, INC.Inventors: Lauren O. Bakaletz, Robert S. Munson, JR., David W. Dyer
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Patent number: 8354246Abstract: The invention provides a method of inhibiting a bacterial RNA polymerases. The invention has applications in control of bacterial RNA polymerase activity, control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, and antibacterial therapy.Type: GrantFiled: December 18, 2009Date of Patent: January 15, 2013Assignee: Rutgers, The State University of New JerseyInventors: Richard H. Ebright, Jayanta Mukhopadhyay, Konstantin Severinov, Ekaterina Semenova
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Patent number: 8354511Abstract: A method is provided for purifying protein having steps of loading a sample containing proteins into a column containing an ion exchanger in a first direction to allow the ion exchanger to absorb proteins; and passing a salt solution through the column in a second direction opposite to the first direction to elute a target protein from the ion exchanger. The method is proven as capable of improving homogeneity of eluted target protein without combining minute and complicated techniques for protein purification. Therefore, the method is convenient, efficient and economic for purifying proteins.Type: GrantFiled: May 25, 2010Date of Patent: January 15, 2013Assignee: Tamkang UniversityInventors: Ming-Kai Chern, Wei-Jhy Shiah, Jyun-Jie Chen, Tzung-You Tsai, Hsin-Yin Lin, Chien-Wei Liu
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Patent number: 8354248Abstract: A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein.Type: GrantFiled: July 9, 2008Date of Patent: January 15, 2013Assignee: Japan Agency for Marine-Earth Science and TechnologyInventors: Yuji Hatada, Yukari Ohta, Yuko Hidaka, Nobuyuki Nakamura
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Publication number: 20130011888Abstract: The invention relates to recombinant geranyl diphosphate (GPP) synthases, genes encoding the synthases, vectors and host cells comprising the same. More particularly, the invention relates to a recombinant GPP synthase, which preferentially facilitates the production of GPP from its isoprenoid precursors and incorporating a recombinant GPP synthase into one or more terpene production pathways of a host organism.Type: ApplicationFiled: March 30, 2011Publication date: January 10, 2013Applicant: CODEXIS, INC.Inventors: Oscar Alvizo, Robert McDaniel, Benjamin Mijts
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Patent number: 8349596Abstract: Provided is a composition containing: an isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 4; and/or a heterodimeric enzyme containing: the isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 4; and an isolated and purified polypeptide containing the amino acid sequence of SEQ ID NO: 2. Also provided is a process for producing a 2-hydroxy-2-methyl carboxylic acid or a salt or ester thereof, wherein the process involves: contacting a 3-hydroxy carboxylic acid or a salt or ester thereof with the above-mentioned composition to produce the 2-hydroxy-2-methyl carboxylic acid or the salt or ester thereof.Type: GrantFiled: November 19, 2010Date of Patent: January 8, 2013Assignee: Evonik Roehm GmbHInventors: Roland H Mueller, Thore Rohwerder
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Publication number: 20130004955Abstract: Newly identified proteins as markers for the detection of ovary tumors, or as therapeutic targets for treatment thereof; affinity ligands capable of selectively interacting with the newly identified markers, methods for tumor diagnosis and therapy using the same.Type: ApplicationFiled: October 26, 2010Publication date: January 3, 2013Applicant: EXTERNAUTICS S.P.A.Inventors: Renata Grifantini, Piero Pileri, Susanna Campagnoli, Alberto Grandi, Matteo Parri, Andrea Pierleoni, Renzo Nogarotto
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Publication number: 20130004979Abstract: The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemo-enzymatic glycorandomization.Type: ApplicationFiled: June 13, 2012Publication date: January 3, 2013Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Jon S. Thorson, Richard W. Gantt, Pauline Marie Jeanne Peltier-Pain
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Patent number: 8343745Abstract: The present invention is directed to a polynucleotide sequence of a novel acylglycerol acyltransferase-like protein MGAT-X1. The invention also provides the human MGAT-X1 associated with the dermatological diseases, urological diseases, muscle-skeleton disorders, hematological diseases, cancer, reproduction disorders, neurological diseases, metabolic diseases, cardiovascular diseases or gastroenterological diseases. The invention also provides assays for the identification of compounds useful for the modulation of dermatological diseases, urological diseases, muscle-skeleton disorders, hematological diseases, cancer, reproduction disorders, neurological diseases, metabolic diseases, cardiovascular diseases or gastroenterological diseases for treating of such diseases associated with expression of the MGAT-X1. The invention also features compounds which bind to and/or activate or inhibit the activity of MGAT-X1 as well as pharmaceutical compositions comprising such compounds.Type: GrantFiled: August 31, 2010Date of Patent: January 1, 2013Assignee: Bayer Pharma AGInventors: Stefan Golz, Ulf Brüggemeier, Bernhard Weingartner, Andreas Geerts
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Publication number: 20120329132Abstract: Improved compositions comprising a cross-linkable protein or polypeptide, and a non-toxic material which induces cross-linking of the cross-linkable protein. The compositions are optionally and preferably prepared in a non-phosphate buffer solvent. Optionally and preferably, the cross-linkable protein includes gelatin and any gelatin variant or variant protein as described herein. Optionally and preferably, the non-toxic material comprises transglutaminase (TG), which may optionally comprise any type of calcium dependent or independent transglutaminase, which may for example optionally be a microbial transglutaminase (mTG).Type: ApplicationFiled: July 23, 2012Publication date: December 27, 2012Inventors: Orahn Preiss BLOOM, Ishay Attar, Natalie Iram, Chagai Komlos, Meir Haber
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Publication number: 20120329108Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: September 5, 2012Publication date: December 27, 2012Applicant: CODEXIS, INC.Inventors: Christopher Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Patent number: 8338672Abstract: A hybrid pepper designated E42.2346 is disclosed. The invention relates to the seeds of hybrid pepper E42.2346 to the plants of hybrid pepper E42.2346 and to methods for producing a hybrid plant, either inbred or hybrid, by crossing the hybrid E42.2346 with itself or another pepper plant. The invention further relates to methods for producing a pepper plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other pepper lines, cultivars or hybrids derived from the hybrid pepper E42.2346.Type: GrantFiled: February 11, 2010Date of Patent: December 25, 2012Assignee: Enza Zaden Beheer B.V.Inventor: Wouter Lindeman
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Patent number: 8338154Abstract: To obtain a mutant protein of an asparagine-linked glycoprotein, which has no N-linked sugar chain under ordinary circumstance, and remains a physiological activity of the glycoprotein before the mutation was introduced, at least one of the amino acids contained in the amino acid sequence motif (I) and/or (II) in the polypeptide of the asparagine-linked glycoprotein is substituted into another amino acid: (I) Asn Xa1 Xa2 (II) Xa3 Val Gly Asn Xa1 Xa2. In amino acid sequence motif (I) and (II), Xa1 represents an amino acid other than Pro, Xa2 represents Thr or Ser and Xa3 represents His or Asp.Type: GrantFiled: November 10, 2010Date of Patent: December 25, 2012Assignee: Seikagaku CorporationInventors: Jin-ichi Inokuchi, Satoshi Uemura
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Patent number: 8338177Abstract: A method for culturing cells in the presence of an alcanoic acid for enhancing protein production.Type: GrantFiled: July 6, 2007Date of Patent: December 25, 2012Assignee: Lonza Biologics PLCInventors: David Mainwaring, Jeremy Wayte
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Publication number: 20120322114Abstract: Heparosan is sulfated at the iduronic acid residue by providing a reaction mixture comprising C5 epimerase to convert glucuronic acid to iduronic acid, and at least one O-sulfotransferase (OST) enzyme and 3?-phosphoadenosine 5?-phosphosulfate (PAPS).Type: ApplicationFiled: March 12, 2012Publication date: December 20, 2012Inventors: Jian Liu, Robert J. Linhardt, Fikri Y. Avci, Eva M. Munoz, Jinghua Chen