Produced By The Action Of A Glycosyl Transferase (e.g., Alpha, Beta, Gamma-cyclodextrins By The Action Of Glycosyl Transferase On Starch, Etc.) Patents (Class 435/97)
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Publication number: 20090269802Abstract: The object of the present invention is to provide ?1,4-galactosyltransferase to transfer a galactose residue to C4 position of galactose residue of lactosylceramide or galactosylceramide, and DNA coding for the enzyme. What is provided includes the following polypeptides (a) and (b), and DNAs encoding thereof: (a) a polypeptide consisting of an amino acid sequence represented by the amino acid Nos. 46-353 in SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a galactose residue from a galactose donor to C4 position of galactose residue of lactosylceramide or galactosylceramide which serves as an acceptor.Type: ApplicationFiled: September 10, 2007Publication date: October 29, 2009Applicant: Seikagaku CorporationInventors: Yoshinao KOJIMA, Satoshi Fukumoto, Keiko Furukawa, Tetsuya Okajima, Koichi Furukawa
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Publication number: 20090269734Abstract: A method in which the recognition specificity of a virus for a receptor sugar chain can be easily determined with a simple instrument or apparatus is provided. In a method for determining the recognition specificity of a virus for a receptor sugar chain or for determining the change in a host infected in accordance with the mutation of virus comprising, a sample of the virus is brought into contact with a support having a polymer with sialo-oligosaccharide immobilized on the surface thereof; and the degree of binding therein is assayed to determine the recognition specificity of said virus for said receptor sugar chain and to determine the change in a host range. The method is suitable for the surveillance of virus.Type: ApplicationFiled: April 21, 2009Publication date: October 29, 2009Inventors: Yasuo SUZUKI, Akira Asai, Takashi Suzuki, Ilpal Jwa, Takeomi Murata, Taiichi Usui, Sou Takeda, Kohei Yamada, Toshitada Noguchi
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Patent number: 7608436Abstract: A process for preparing saccharide oligomers uses an aqueous feed composition that comprises at least one monosaccharide or linear saccharide oligomer, and has a solids concentration of at least about 70% by weight. The feed composition is heated to a temperature of at least about 40° C., and is contacted with at least one catalyst that accelerates the rate of cleavage or formation of glucosyl bonds, such as enzyme or acid, for a time sufficient to cause formation of non-linear saccharide oligomers. A product composition is produced that contains a higher concentration of non-linear saccharide oligomers than linear saccharide oligomers.Type: GrantFiled: January 25, 2006Date of Patent: October 27, 2009Assignee: Tate & Lyle Ingredients Americas, Inc.Inventors: Michael D. Harrison, James C. Purdue, Penelope A. Patton, Andrew J. Hoffman, James M. Gaddy, Chi-Li Liu, Robert V. Schanefelt
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Patent number: 7588922Abstract: Nucleic acid molecules are described which encode polypeptides having the enzymatic activity of a fructosyltransferase. Also, vectors, host cells and transgenic plants are described which contain such nucleic acid molecules. Furthermore, processes for producing polyfructose, particularly that of the inulin type, using the hosts described and/or the fructosyltransferase produced by them are described.Type: GrantFiled: January 19, 2005Date of Patent: September 15, 2009Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften E.V.Inventors: Arnd G Heyer, Jochen Rehm, Regina Wendenburg
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Publication number: 20090215127Abstract: The present invention relates to a process for producing downstream products, such as fermentable sugars (e.g., glucose) and alcohols (e.g., ethanol) from starch-containing material (e.g., grain) without a pH adjustment before or after the starch liquefaction step.Type: ApplicationFiled: February 5, 2009Publication date: August 27, 2009Applicant: DANISCO US INC., GENENCOR DIVISIONInventors: Suzanne Breneman, Brad A. Paulson, Vivek Sharma, Jayarama K. Shetty
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Publication number: 20090215116Abstract: The invention relates to ?-1,3-N-acetylgalactosaminyltransferase polypeptides, nucleic acids that encode the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: April 7, 2006Publication date: August 27, 2009Applicant: National Research Council of CanadaInventors: Michel Gilbert, Warren Wakarchuk, Scott Houliston
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Patent number: 7579173Abstract: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate or chondroitin or heparin/heparosan synthases from Pasteurella, in order to create a variety of glycosaminoglycan oligosaccharides having a natural or chimeric or hybrid sugar structure with a targeted size that are substantially monodisperse in size.Type: GrantFiled: January 9, 2007Date of Patent: August 25, 2009Assignee: The Board of Regents of the University of OklahomaInventors: Paul L. DeAngelis, Wei Jing
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Patent number: 7575904Abstract: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate synthase from Pasteurella multocida. The present invention also relates to coatings for biomaterials wherein the coatings provide protective properties to the biomaterial and/or act as a bioadhesive. Such coatings could be applied to electrical devices, sensors, catheters and any device which may be contemplated for use within a mammal. The present invention further relates to drug delivery matrices which are biocompatible and may comprise combinations of a biomaterial or a bioadhesive and a medicament or a medicament-containing liposome. The biomaterial and/or bioadhesive is a hyaluronic acid polymer produced by a hyaluronate synthase from Pasteurella multocida.Type: GrantFiled: July 11, 2005Date of Patent: August 18, 2009Assignee: The Board of Regents of the University of OklahomaInventor: Paul L. DeAngelis
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Patent number: 7527949Abstract: The invention is directed to immunogenic compositions comprising polysaccharides and polysaccharide-protein conjugates that are useful to induce or detect the production of antibodies specific for H. pylori. The invention is also directed to methods of purifying the H. pylori polysaccharides from bacteria. The polysaccharide may be incorporated into an immunogenic composition or used in vitro to assay for the presence of H. pylori antibodies in a sample or biological fluid.Type: GrantFiled: October 28, 2004Date of Patent: May 5, 2009Assignee: Wyeth Holdings CorporationInventors: Mario Artur Monteiro, James Fulginiti, Deborah Ann Dilts
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Patent number: 7524655Abstract: This invention provides nucleic acid and amino acid sequences of fucosyltransferases from Helicobactor pylori. The invention also provides methods to use the fucosyltransferases to synthesize oligosaccharides, glycoproteins, and glycolipids.Type: GrantFiled: December 6, 2005Date of Patent: April 28, 2009Assignees: Neose Technologies, Inc., Governors of the University of AlbertaInventors: Karl F. Johnson, Daniel James Bezila, Diane E. Taylor, Joanne Simala-Grant, David Rasko
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Publication number: 20090104666Abstract: The present invention relates to hybrid glycosylated products, and in particular, to natural products such as polyketides and glycopeptides, and to processes for their preparation. The invention is particularly concerned with recombinant cells in which a cloned microbial glycosyltransferase can be conveniently screened for its ability to generate specific glycosylated derivatives when supplied with polyketide, peptide, or polyketide-peptides as substrates. The invention demonstrates that cloned glycosyltransferases when rapidly screened for their ability to attach a range of activated sugars to a range of exogenously supplied or endogenously generated aglycone templates, show a surprising flexibility towards both aglycone and sugar substrates, and that this process allows the production of glycosylated polyketides in good yield.Type: ApplicationFiled: December 18, 2008Publication date: April 23, 2009Inventors: Peter Francis Leadlay, James Staunton, Sapine Gaisser
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Patent number: 7521212Abstract: The invention concerns the production by microbiological process of oligopolysaccharides of biological interest. More particularly, the invention concerns a method for synthesizing in vivo oligopolysaccharides by internalization of an exogenous precursor in growing bacterial cells expressing adequate modifying and glycosylating genes.Type: GrantFiled: July 7, 2000Date of Patent: April 21, 2009Assignee: Centre National de la Recherche Scientifique (CNRS)Inventors: Eric Samain, Bernard Priem
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Publication number: 20090082307Abstract: The invention concerns the production by microbiological process of oligopolysaccharides of biological interest. More particularly, the invention concerns a method for synthesizing in vivo the oligopolysaccharides by internalization of an exogenous precursor in growing bacterial cells expressing adequate modifying and glycosylating genes.Type: ApplicationFiled: October 31, 2007Publication date: March 26, 2009Inventors: Eric Samain, Bernard Priem
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Publication number: 20090076258Abstract: The invention relates to an enzymatic method for synthesizing oligosaccharides, whereby one saccharide group of a sucrose analogue each is transferred onto an acceptor molecule, for example for glycosylating a hydroxyl compound, a saccharide, peptide, or a drug. According to the inventive method, an enzymatic synthesis of ?-D-fructofuranosyl-a-D-aldopyranoside is carried out in a first step, and in a second step one of the saccharide groups is enzymatically transferred onto the acceptor molecule.Type: ApplicationFiled: March 22, 2006Publication date: March 19, 2009Inventors: Klaus Buchholz, Jurgen Seibel, Hans-Joachim Joerdening, Raphael Beine
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Patent number: 7491518Abstract: A novel method of very efficiently elevating the yield of oligosacchrides containing ?-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific ?-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an ?-galactosidase and thus performing a dehydrocondensation.Type: GrantFiled: August 8, 2001Date of Patent: February 17, 2009Assignees: Amano Enzyme Inc., Bio Research Corporation of Yokohama, Ensuiko Sugar Refining Co., Ltd.Inventors: Masamichi Okada, Shigeharu Mori, Hiroyuki Hashimoto, Koki Fujita, Kozo Hara
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Patent number: 7455996Abstract: Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.Type: GrantFiled: January 14, 2005Date of Patent: November 25, 2008Assignee: Ajinomoto Co., Inc.Inventors: Chieko Osumi, Jinshi Nozaki, Takao Kida
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Publication number: 20080286410Abstract: A process for producing a starch comprises treating a feed starch that comprises amylopectin with glucanotransferase to produce a chain-extended starch, treating the chain-extended starch with a debranching enzyme to produce a starch product that comprises amylose fragments, crystallizing at least part of the starch product, heating the starch product in the presence of moisture, treating the starch product with alpha-amylase, and washing the starch product to remove at least some non-crystallized starch. The product of this process has a relatively high total dietary fiber content.Type: ApplicationFiled: January 8, 2008Publication date: November 20, 2008Inventors: Patricia A. Richmond, Eric A. Marion, Thomas Eilers, Annette Evans, Xian-Zhong Han, Shakeel Ahmed, Donald W. Harris
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Patent number: 7396666Abstract: A tumor marker nucleic acid of the present invention is concerned with a nucleic acid hybridizing under stringent conditions to a nucleotide sequence described in SEQ ID NO: 1 or a complementary nucleotide sequence thereof. A method of testing canceration of the present invention is a method comprising diagnosing a biological sample as being cancerous when the transcription level of the nucleic acid in the biological sample significantly exceeds that in a normal biological sample as a control. The present invention also relates to a ?1,3-N-acetyl-D-glucosaminyltransferase protein having an activity of transferring N-acetyl-D-glucosamine from a donor substrate to an acceptor substrate through ?1,3-linkage.Type: GrantFiled: December 26, 2003Date of Patent: July 8, 2008Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Hisashi Narimatsu, Takashi Kudo, Akira Togayachi, Toru Hiruma
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Publication number: 20080145899Abstract: The present invention relates to the enzymatic synthesis of oligosaccharides, including sialylated product saccharides. In particular, it relates to the use of recombinant cells to take up low cost precursors such as glucose, pyruvate and N-actyl-glucosamine, and to synthesize activated sugar moieties that are used in oligosaccharide synthesis. The methods make possible the synthesis of many oligosaccharides using microorganisms and readily available, relatively inexpensive starting materials.Type: ApplicationFiled: September 19, 2005Publication date: June 19, 2008Applicant: Neose Technologies IncInventors: Karl Johnson, Noel J. Byrne, Shawn DeFrees
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Patent number: 7335500Abstract: This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.Type: GrantFiled: November 19, 2001Date of Patent: February 26, 2008Assignee: The Scripps Research InstituteInventors: Chi-Huey Wong, Yoshitaka Ichikawa, Gwo-Jenn Shen, Kun-Chin Liu
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Patent number: 7309587Abstract: The present invention can provide a process for producing a protein having ?1,3-N-acetylglucosaminyltransferase activity using a transformant comprising a DNA encoding a protein having ?1,3-N-acetylglucosaminyltransferase activity derived from a microorganism belonging to the genus Pasteurella and a process for producing an N-acetylglucosamine-containing complex carbohydrate using a transformant capable of producing a protein having ?1,3-N-acetylglucosaminyltransferase activity derived from a microorganism.Type: GrantFiled: October 25, 2002Date of Patent: December 18, 2007Assignee: Kyowa Jakko Kogyo Co., Ltd.Inventors: Tetsuo Endo, Satoshi Koizumi
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Publication number: 20070254344Abstract: The present invention provides methods which enables synthesis of various sugar chains and products obtained by the same. More specifically, the present invention provides protected sugar chain compounds represented by the formula (I) below: [wherein R1 and R2 are the same or different and each is a linear or branched sugar chain, S1 is any sugar residue, SA and SB are the same or different sugar residues, L is a bond or a linear sugar chain, X is absent, or, if present, represents certain group, the sugar residues SA and SB are cleaved by different exoglycosidases, respectively] and libraries thereof, and methods of producing the same; methods of producing a sugar chain compound, which comprises treating the sugar chain compound or library with glycosidase, and glycosidase decomposition products obtained by the same; intermediates for the synthesis of protected sugar chain compounds; reagents and kits; and the like.Type: ApplicationFiled: March 22, 2007Publication date: November 1, 2007Inventors: Ichiro Matsuo, Yukishige Ito
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Patent number: 7256029Abstract: A novel strain of Lactococcus lactis subspecies cremori (“Ropy 352”) has been identified and isolated. Ropy 352 produces a previously unknown exopolysaccharide (EPS 352) that when expressed or added to milk, imparts highly desirable sensory characteristics to the milk, including making the milk very thick, with a very smooth mouth-feel, and slightly sweet with an obvious “chewable-bite”.Type: GrantFiled: February 2, 2001Date of Patent: August 14, 2007Assignees: United States of America, as Represented by the Secretary of Agriculture, The State of Oregon by and Through the Oregon State Board of Higher Education on behalf of Oregon State UniversityInventors: Janine E. Trempy, Eric P. Knoshaug, William E. Sandine, Jeff A. Ahlgren, Karen P. Dierksen
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Patent number: 7247456Abstract: This invention provides methods for practical enzymatic conversion of GDP-mannose to GDP-fucose. These methods are useful for efficient synthesis of reactants used in the synthesis of fucosylated oligosaccharides.Type: GrantFiled: July 25, 2002Date of Patent: July 24, 2007Assignee: Neose Technologies, Inc.Inventor: Eric R. Sjoberg
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Patent number: 7241607Abstract: The present invention relates to a novel microorganism and a method for producing fructooligosaccharides and neofructooligosaccharides. More particularly, the present invention relates to Penicillium citrinum KCTC 10225BP of soil origin which produces fructosyl transferase and hydrolyzes sucrose into fructooligosaccharides of the following formula I: in which n is integer of 1 to 5, G represents glucose and F represents fructose, and neofructooligosaccharides of the following formula II: in which n is integer of 1 to 5, G and F are defined as above, at the same time using the fructosyl transferase, and to a method for producing simultaneously fructooligosaccharides and neofructooligosaccharides using said microorganism.Type: GrantFiled: May 18, 2004Date of Patent: July 10, 2007Assignee: CJ CorporationInventors: Joon Sang Han, Kang June Park, Dae Sub Shin, Jung Hoon Kim, Jin Chul Kim, Ki Chang Lee, Woon Hwa Lee, Seung Wook Kim, Seung Won Park
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Patent number: 7241605Abstract: According to the present invention, there can be provided a novel polypeptide having ?1,3-galactosyltransferase activity involved in the synthesis of type 1 sugar chains, a DNA coding for said polypeptide, a recombinant vector comprising said DNA, a transformant carrying said recombinant vector, a process for producing type 1 sugar chain-containing sugar chains and complex carbohydrates by use of said polypeptide or said transformant, an antibody recognizing said polypeptide, a method for detecting or quantifying said polypeptide by use of said antibody, a method for screening a substance correlated with said polypeptide, a method for diagnosis or treatment of cancers in the digestive system by use of said DNA or said antibody, and a method for treatment of cancers in the digestive system by use of a substance obtained by said screening method.Type: GrantFiled: February 24, 2000Date of Patent: July 10, 2007Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hisashi Narimatsu, Soichiro Isshiki, Akira Togayachi, Katsutoshi Sasaki
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Patent number: 7223570Abstract: The object of the present invention is to provide a novel glycosyl derivative of cyclotetrasaccharide represented by cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?}, and it is solved by providing a branched cyclotetrasaccharide, wherein one or more hydrogen atoms in the hydroxyl groups of cyclotetrasaccharide are replaced with an optionally substituted glycosyl group, with the proviso that, when only one hydrogen atom in the C-6 hydroxyl group among the above hydrogen atoms is substituted with an optionally-substituted glycosyl group, the substituted glycosyl group is one selected from those excluding D-glucosyl group.Type: GrantFiled: March 8, 2002Date of Patent: May 29, 2007Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Hajime Aga, Takanobu Higashiyama, Hikaru Watanabe, Tomohiko Sonoda, Michio Kubota
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Patent number: 7223571Abstract: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate or chondroitin or heparin/heparosan synthases from Pasteurella, in order to create a variety of glycosaminoglycan oligosaccharides having a natural or chimeric or hybrid sugar structure with a targeted size that are substantially monodisperse in size.Type: GrantFiled: August 15, 2003Date of Patent: May 29, 2007Assignee: The Board of Regents of the Universtiy of OklahomaInventors: Paul L. DeAngelis, Wei Jing
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Patent number: 7211663Abstract: One aspect of the present invention relates to differentially protected glycosyl phosphates. Another aspect of the present invention relates to the preparation of glycosyl phosphates from glycal precursors. In another aspect of the present invention, glycosyl phosphates are used as glycosyl donors in glycosylation reactions.Type: GrantFiled: November 1, 2001Date of Patent: May 1, 2007Assignee: Massachusetts Institute of TechnologyInventors: Peter H. Seeberger, Obadiah J. Plante
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Patent number: 7192746Abstract: The object of the present invention is to provide an ?-isomaltosyl-transferring enzyme which forms a cyclotetrasaccharide having the structure of cyclo{?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?6)-?-D-glucopyranosyl-(1?3)-?-D-glucopyranosyl-(1?} from a saccharide having a glucose polymerization degree of at least three and having both the ?-1,6 glucosidic linkage as a linkage at the non-reducing end and the ?-1,4 glucosidic linkage other than the linkage at the non-reducing end; microorganisms which produce the enzyme; process for producing the enzyme; cyclotetrasaccharide or saccharide compositions comprising the same; and uses thereof.Type: GrantFiled: May 22, 2001Date of Patent: March 20, 2007Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Michio Kubota, Tomoyuki Nishimoto, Hajime Aga, Shigeharu Fukuda, Toshio Miyake
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Patent number: 7166450Abstract: The present invention relates to a method of producing hyaluronic acid that includes introducing a purified nucleic acid segment having a coding region encoding enzymatically active hyaluronan synthase into a Bacillus strain, and culturing the Bacillus strain under conditions appropriate for the production of hyaluronic acid.Type: GrantFiled: June 5, 2006Date of Patent: January 23, 2007Assignee: The Board of Regents of the University of OklahomaInventors: Paul H. Weigel, Kshama Kumari, Paul DeAngelis
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Patent number: 7166449Abstract: A bacterial ?1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing ?1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohy-drate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.Type: GrantFiled: April 9, 2002Date of Patent: January 23, 2007Assignee: The Governors of the University of AlbertaInventors: Diane E. Taylor, Zhongming Ge
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Patent number: 7153674Abstract: Described are nucleic acid molecules encoding enzymes having fructosyl polymerase activity. These enzymes are sucrose dependent sucrose fructosyltransferases (SST) enzymes. Furthermore, vectors and host cells are described containing the nucleic acid molecules, in particular transformed plant cells and plants that can be regenerated from them and that express the described SSTs. Furthermore, methods for the production of short-chain fructosyl polymers using the described hosts and/or the SSTs produced by them are described.Type: GrantFiled: November 14, 2002Date of Patent: December 26, 2006Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.Inventors: Arnd G. Heyer, Elke Hellwege, Dominique Gritscher
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Patent number: 7153677Abstract: The present invention relates to a recombinantly produced, enzymatically active hyaluronan synthase, wherein the recombinantly produced, enzymatically active hyaluronan synthase is a single protein that is a dual-action catalyst that utilizes UDP-GlcA and UDP-GlcNAc to synthesize HA. The recombinantly produced, enzymatically active hyaluronan synthase may be produced from a hyaluronan synthase gene isolated from a microbial source, such as a streptococcal source.Type: GrantFiled: June 5, 2006Date of Patent: December 26, 2006Assignee: The Board of Regents of the University of OklahomaInventors: Paul H. Weigel, Kshama Kumari, Paul DeAngelis
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Patent number: 7141409Abstract: The present invention relates to a recombinant host cell that includes an expression construct having a hyaluronan synthase gene that encodes an enzymatically active hyaluronan synthase (HAS), and to the use of these recombinant cells that produce hyaluronan synthase and its hyaluronic acid product.Type: GrantFiled: June 5, 2006Date of Patent: November 28, 2006Assignee: The Board of Regents of the University of OklahomaInventors: Paul H. Weigel, Kshama Kumari, Paul DeAngelis
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Patent number: 7094530Abstract: The invention provides a novel species of ?-1,3-fucosyltransferase expressed by a gene cloned from animal cells, a cDNA coding for the ?-1,3-fucosyltransferase, a method of detecting, or inhibiting the production of, the ?-1,3-fucosyltransferase which involves the use of the cDNA, a recombinant vector containing the DNA as an insert, a cell harboring the recombinant vector, and a method of producing same. The ?-1,3-fucosyltransferase of the invention is useful in the production of carbohydrate chains having useful physiological activity, for example sialyl Lewis x, and modifications thereof.Type: GrantFiled: November 29, 1994Date of Patent: August 22, 2006Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Katsutoshi Sasaki, Kazumi Miura, Nobuo Hanai, Tatsunari Nishi
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Patent number: 7087746Abstract: Disaccharides of formula (I) are derived from a-maltosyl fluoride, wherein R1 and R2 are defined. Such disaccharides are useful as glycosyl donors in transglycosylation reactions catalyzed by glycoside-hydrolazes or in transfer reactions catalyzed by transglycosylases during the preparation of oligosaccharides or polysaccharides.Type: GrantFiled: July 26, 2002Date of Patent: August 8, 2006Assignee: Centre National de la Recherche Scientifique (C.N.R.S.)Inventors: Lionel Greffe, Hugues Driguez
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Patent number: 7083963Abstract: Nucleic acid molecules are described encoding proteins with the enzymatic activity of a fructosyl transferase. These enzymes are fructosyl transferases (FFT). Moreover, vectors and host cells are described containing the nucleic acid molecules of the invention, in particular transformed plant cells, plant tissue and plants regenerable therefrom, which express the described FFT. Furthermore, methods for the production of long-chain inulin by using the described proteins, hosts, in particular the plant cells and/or FFT produced by them, are described.Type: GrantFiled: March 13, 2003Date of Patent: August 1, 2006Assignee: Max-Planck Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Arnd G Heyer, Elke Hellwege, Dominique Gritscher
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Patent number: 7041484Abstract: The invention provides a novel starch branching enzyme that is bound to A-type starch granules in wheat, barley, rye or triticale. The enzyme is not substantially associated with B-type starch granules. A cDNA sequence encoding an isoform of the enzyme has been isolated from the wheat cultivar Fielder and deduced amino acid sequence has been determined.Type: GrantFiled: October 27, 2000Date of Patent: May 9, 2006Assignee: National Research Council of CanadaInventors: Monica Baga, Mingsheng Peng, Ramesh B. Nair, Anne Repellin, Ming Gao, Pierre Hucl, Graham J. Scoles, Ravindra N. Chibbar
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Patent number: 7019131Abstract: The reactivity of a number of p-methylphenyl thioglycoside (STol) donors which are either fully protected or have one hydroxyl group exposed has been quantitatively determined by HPLC in conjunction with the development of a broadly applicable approach for a facile one-pot synthesis of oligosaccharides. The influence on reactivity of the structural effects of different monosaccharide cores and different protecting groups on each glycoside donor is characterized and quantified. In addition, a correlation between glycosyl donor reactivity and the chemical shift of the anomeric proton by 1 H NMR has been established. A database of thioglycosides as glycosyl donors has been created using this reactivity data. The utility is demonstrated by the easy and rapid one-pot assembly of various linear and branched oligosaccharide structures.Type: GrantFiled: March 25, 2003Date of Patent: March 28, 2006Assignee: The Scripps Research InstituteInventors: Chi-Huey Wong, Zhiyuan Zhang, Ian Ollmann, Timor Baasov, Xin-Shan Ye
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Patent number: 7015318Abstract: The subject of the invention is a process for modifying starch or starch derivatives by branching enzymes, consisting in continuously introducing the said branching enzymes into the reaction medium containing starch or starch derivatives.Type: GrantFiled: November 20, 2002Date of Patent: March 21, 2006Inventors: Patrick Fuertes, Carole Petitjean
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Patent number: 7005279Abstract: The present invention provides a novel polypeptide having a ?1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.Type: GrantFiled: June 29, 2000Date of Patent: February 28, 2006Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Katsutoshi Sasaki, Norihiko Shiraishi, Ayumi Natsume, Yoji Yamada, Satoshi Nakagawa, Susumu Sekine
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Patent number: 7001994Abstract: Methods to introduce highly phosphorylated mannopyranosyl oligosaccharide derivatives containing mannose 6-phosphate (M6P), or other oligosaccharides bearing other terminal hexoses, to carbonyl groups on oxidized glycans of glycoproteins while retaining their biological activity are described. The methods are useful for modifying glycoproteins, including those produced by recombinant protein expression systems, to increase uptake by cell surface receptor-mediated mechanisms, thus improving their therapeutic efficacy in a variety of applications.Type: GrantFiled: January 17, 2002Date of Patent: February 21, 2006Assignee: Genzyme CorporationInventor: Yunxiang Zhu
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Patent number: 6972189Abstract: The present invention relates to a novel microorganism and a method for producing fructooligosaccharides and neofructooligosaccharides. More particularly, the present invention relates to Penicillium citrinum KCTC 10225BP of soil origin which produces fructosyl transferase and hydrolyzes sucrose into fructooligosaccharides of the following formula I: in which n is integer of 1 to 5, G represents glucose and F represents fructose, and neofructooligosaccharides of the following formula II: in which n is integer of 1 to 5, G and F are defined as above, at the same time using the fructosyl transferase, and to a method for producing simultaneously fructooligosaccharides and neofructooligosaccharides using said microorganism.Type: GrantFiled: May 13, 2002Date of Patent: December 6, 2005Assignee: CJ CorporationInventors: Joon Sang Han, Kang June Park, Dae Sub Shin, Jung Hoon Kim, Jin Chul Kim, Ki Chang Lee, Woon Hwa Lee, Seung Wook Kim, Seung Won Park
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Patent number: 6911326Abstract: The present invention relates to a polypeptide having the activity of N-acetylglucosamine-1-phosphate uridyltransferase (hereinafter referred to as GlmU), a DNA coding for the polypeptide, a recombinant DNA containing the DNA, a transformant carrying the recombinant DNA, a method of culturing the transformant for producing the GlmU polypeptide, and a method of culturing the transformant for producing uridine 5?-diphosphate-N-acetylglucosamine. According to the present invention, mass-scale production of the GlmU polypeptide derived from microorganisms belonging to the genus Corynebacterium glutamicum has been enabled by genetic recombinant technology. By using the enzyme, uridine 5?-diphosphate-N-acetylglucosamine can be produced efficiently.Type: GrantFiled: November 17, 2000Date of Patent: June 28, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kazuo Nagai, Masaaki Wachi
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Patent number: 6884604Abstract: The present invention provides combinatorial methods for rapidly generating a diverse library of glycorandomized structures, comprising incubating one or more aglycons and a pool of NDP-sugars in the presence of a glycosyltransferase. The glycosyltransferase may be one that is associated with or involved in production of natural secondary metabolites, or one which is putatively associated with or involved in production of natural secondary metabolites. The glycosyltransferase may show significant flexibility with respect to its NDP-sugar donors and/or its aglycons. NDP-sugar donors may be commercially available, or may be produced by utilizing mutant or wild type nucleotidyltransferases significant flexibility with respect to their substrates.Type: GrantFiled: April 1, 2002Date of Patent: April 26, 2005Assignee: Sloan-Kettering Institute for Cancer ResearchInventor: Jon S. Thorson
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Patent number: 6867026Abstract: The present invention describes a protein having glucosyltransferase activity. This protein is derived from lactobacilli, which are food-grade microorganisms with the Generally Recognized As Safe (GRAS) status. The protein produces a glucan with a unique structure having 4-linked, 6-linked and 4,6-linked anhydroglucose units or in the presence of suitable acceptors, oligosaccharides. According to the invention lactobacilli capable of producing this glucan using the novel glucosyltransferase can be used as a probiotic or symbiotic.Type: GrantFiled: November 29, 2001Date of Patent: March 15, 2005Assignee: Nederlandse Organisatie Voor Toegepast Natuurwetenschappelijk Onderzoek TNOInventors: Gerritdina Hendrika Van Geel-Schutten, Lubbert Dijkhuizen, Hakim Rahaoui, Robert-Jan Leer
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Patent number: 6867194Abstract: Sugar-modified SIN-1 compositions are provided. The compositions are useful for generating NO in response to hydrolytic activity of a glycosidase specific for the O-glycosidic bond between the sugar and SIN-1 moieties. Pharmaceutical compositions containing the sugar-modified SIN-1 compositions and methods of using the compositions are also provided.Type: GrantFiled: August 9, 2001Date of Patent: March 15, 2005Assignee: Wayne State UniversityInventors: Peng George Wang, Xuejun Wu, Xiaoping Tang
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Patent number: 6864063Abstract: The invention relates to the use of modified starch obtainable by treating amylose containing starch in aqueous medium with an enzyme from the group of the ?-1,4-?-1,4-glucosyl transferases (EC 2.4.1.25) or an enzyme the activity of which corresponds to that of enzymes from the group just mentioned, as an agent for forming a thermoreversible gel. The invention also relates to products in the form of a thermoreversible gel having as gel-forming substance a modified starch as defined. The invention further relates to the use of a modified starch as defined in the form of an aqueous solution.Type: GrantFiled: April 3, 2002Date of Patent: March 8, 2005Assignee: Cooperatieve Verkoop-en Productievereniging van Aardappelmeel en Derivaten AVEBE B.A.Inventors: Gerrit Jan Willem Euverink, Doede Jacob Binnema
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Patent number: 6852833Abstract: The present invention provides an artificial chaperon useful for refolding the proteins having low voluntary folding ability and being difficult or unable to be a native form without a second (or assistant) of a molecular chaperon in a short time, and folding said proteins as an active form. The present invention relates to an artificial chaperon kit characterized in that the kit comprises cyclic saccharide cycloamylose and polyoxyethylenic detergent or cyclic saccharide cycloamylose and ionic detergent. The present invention also relates to a method for diluting the denaturant making the protein a denatured state by adding a specific detergent to a denatured protein, and preventing protein molecules from aggregation, thereafter adding cyclic saccharide cycloamylose, utilizing the inclusion ability thereof to strip detergent, accelerating the proper folding of protein into a correct higher-order structure with activity.Type: GrantFiled: August 10, 2000Date of Patent: February 8, 2005Assignees: Director of National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Bio-oriented Technology Research Advancement InstitutionInventors: Sachiko Machida, Kiyoshi Hayashi