Viscosity Or Degree Of Polymerization Changed (e.g., Degrading, Cross Linking, Etc.) Patents (Class 536/41)
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Patent number: 5283353Abstract: The invention resides in oligosaccharide derivatives which are obtained by the enzynmatic cleavage of carbohydrates linked to proteins through asparagine groups. The oligosaccharide moieties of the instant invention are 1-amino-1-deoxyoligosaccharide compounds which are cleaved from a glycoprotein with an aminohydrolase enzyme, wherein the amino group of the oligosaccharide is subsequently substituted with a fluorescent reporter compound. The compounds of the instant invention are characterized by the glycosylamine bonds exhibiting less than 10% hydrolysis at 25.degree. C. in aqueous solution over a two days at pH values between 4-10.Type: GrantFiled: June 5, 1990Date of Patent: February 1, 1994Assignees: Genzyme Corporation, Purdue Research FoundationInventors: James R. Rasmussen, John M. Risley, Robert L. Van Etten
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Patent number: 5283328Abstract: The present invention relates to compounds of the formula I[CH.sub.3 --(CH.sub.2).sub.29 --O--].sub.m --R (I)whereinif m stands for 1, thenR represents a group derived from a mono- or di- or oligosaccharide by removing the hydrogen atom from the hydroxyl group being in the 1(alpha) or 1(beta) position, or the O-protected, preferably acetylated derivative thereof,if m stands for 2, thenR represents a group derived by removing a hydrogen atom attached to the carbon atom being in position 1 of the reaction product of glucose and mono-, di- or triethylene glycol or the O-protected, preferably O-acetylated derivative thereof.Type: GrantFiled: October 11, 1994Date of Patent: February 1, 1994Assignee: Biogal Gyogyszergyar Rt.Inventors: Antal Kovacs, Andras Liptak, Pal Nanasi, Zoltan Szurmai, Istvan Csernus, Katalin Marossy, Katalin Kovacs-Hadadi, Zsuzsa Emri-Harsi, Ildiko Gombos-Nemeti
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Patent number: 5281702Abstract: The invention relates to N-deacetyl-lysogangliosides and physiologically acceptable salts thereof, especially those of formula (I): ##STR1## wherein R represents --CH.dbd.CH(CH.sub.2)nCH.sub.3 or --CH.sub.2 CH.sub.2 (CH.sub.2)nCH.sub.3 ;n is 12 or 14;R.sup.1 represents ##STR2## or hydrogen; and R.sup.2 represents hydrogen or acetyl; for use in therapy.The compounds are useful as inhibitors of phospholipases A.sub.2, and of superoxide production, as antiproliferative and immunosuppressant agents in the treatment of autoimmune diseases. The invention also relates to the use of N-deacetyl-lysogangliosides for the manufacture of medicaments for the treatment of the aforementioned conditions.Type: GrantFiled: June 18, 1991Date of Patent: January 25, 1994Assignee: Wellcome Foundation LimitedInventors: Ezio Tubaro, Giovanni Cavallo
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Patent number: 5280111Abstract: The present invention relates to novel tocopheryl oligosaccharides, acylated tocopheryl oligosaccharides, sulfated tocopheryl oligosaccharides, and antiviral agents including the sulfated tocopheryl oligosaccharides as active ingredients. In the tocopheryl oligosaccharide, the hydrogen atom of the hydroxy group at the 1-position in the terminal sugar moiety of an oligosaccharide which consists of 3.about.20 of identical or different repeating monosaccharide units selected from the group consisting of glucose, galactose, mannose, talose, idose, altrose, allose, glucose, xylose, arabinose, rhamnose, fucose, and fructose, which are glycoside-linked, is substituted by a tocopherol group. In the acylated tocopheryl oligosaccharides, each of any hydroxy group of the sugar moiety, other than the hydroxy group at the 1-position in the terminal sugar moiety of the oligosaccharide described above, is protected by an acyl group.Type: GrantFiled: March 24, 1992Date of Patent: January 18, 1994Assignee: Dainippon Ink and Chemicals, Inc.Inventors: Tadao Shoji, Naoya Ikushima, Kaname Katsuraya, Nahoko Takahashi, Fusayo Kobayashi, Toshiyuki Uryu, Takashi Yoshida, Naoki Yamamoto, Hideki Nakashima
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Patent number: 5278299Type: GrantFiled: March 18, 1991Date of Patent: January 11, 1994Assignee: Scripps Clinic and Research FoundationInventors: Chi-Huey Wong, Yoshitaka Ichikawa, Gwo-Jenn Shen
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Patent number: 5275939Abstract: The present invention provides a process for preparing asialo G.sub.M1 characterized in that neuraminidase isozyme L is allowed to act on gangliosides to produce asialo G.sub.M1.Type: GrantFiled: June 28, 1991Date of Patent: January 4, 1994Assignee: Marukin Shoyu Co., Ltd.Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
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Patent number: 5268461Abstract: An alkylated oligosaccharide and the acetyl derivative of the same, both useful as the raw material for producing lowly toxic surfactants or medicines. The alkylated oligosaccharide is prepared by substituting with an alkyl group having a linear or branched chain the hydrogen atom of the hydroxy group at the 1-position of a terminal sugar moiety of an oligosaccharide in which glucose moieties are .beta.(1.fwdarw.3)-glucoside-linked, or of an oligosaccharide in which galactose is .beta.(1.fwdarw.4)-glycoside-linked at the 4-position in the galactose moiety of lactose and in which galactose moieties are .beta.(1.fwdarw.4)-glycoside-linked in succession to the newly formed terminal galactose moieties. The invention also includes an acetyl derivative of the oligosaccharide, wherein the hydroxy groups excluding the 2-positional hydroxy group adjacent to the alkyl-ether-linking position in the terminal sugar moiety are acetylated, and a mixture thereof.Type: GrantFiled: February 25, 1992Date of Patent: December 7, 1993Assignee: Dainippon Ink & Chemicals, Inc.Inventors: Tadao Shoji, Nahoko Takahashi, Koichiro Adachi, Naoya Ikushima, Kaname Katsuraya, Toshiyuki Uryu, Takashi Yoshida
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Patent number: 5268364Abstract: O-glycosylation and O-glycosylation extension inhibitors influence selectin-dependent interactions between cells and between cells and platelets.Type: GrantFiled: December 12, 1991Date of Patent: December 7, 1993Assignee: The Biomembrane InstituteInventors: Naoya Kojima, Kazuko Handa, Sen-Itiroh Hakomori
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Patent number: 5264424Abstract: N-acyl-N,N'-di-lysogangliosides, N'-acyl-N,N'-di-lysogangliosides and N,N'-diacyl-N,N'-di-lysogangliosides, in which the acyl groups are derived from an organic acid of the aliphatic, aromatic, araliphatic, alicyclic or heterocyclic series and in which at least one of the two acyl groups is not aliphatic, and their preparation are disclosed. Also disclosed is the preparation of the esters, inner esters, amides and hydroxy peracylates of these compounds and salts thereof. These compounds are useful in the treatment of pathologies of the central and peripheral nervous systems.Type: GrantFiled: November 13, 1990Date of Patent: November 23, 1993Assignee: Fidia S.p.A.Inventors: Francesco Della Valle, Aurelio Romeo
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Patent number: 5264345Abstract: The present invention provides a process for the specific determination of pancreatic .alpha.-amylase in the presence of salivary .alpha.-amylase in body fluids by reaction with a system for the detection of .alpha.-amylase with the use of an inhibitor for salivary .alpha.-amylase, wherein, as substrate, there is used a compound of the general formula: ##STR1## in which R.sub.1 is a straight-chained or branched alkyl or alkoyl radical containing up to 6 carbon atoms, a cycloalkyl or cycloalkoxyl radical containing 3 to 6 carbon atoms or a benzoyl, benzyl or phenyl radical which is optionally hydrophilically substituted, R.sub.2 is a hydrogen atom or in which R.sub.1 and R.sub.2 together form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, Gluc is a glucose molecule, n is 1, 2 or 3 and X is an optically determinable residue.Type: GrantFiled: September 4, 1990Date of Patent: November 23, 1993Assignee: Boehringer Mannheim GmbHInventors: Axel Schmidt, Elli Rauscher, Herbert von der Eltz
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Patent number: 5256788Abstract: The present invention relates to stabilizing agents for enzymes which comprises an enzyme stabilizing amount of an N-substituted moranoline derivative of the formula (III): ##STR1## wherein R.sup.1 is phenyl lower alkynyl, phenoxy lower alkenyl or phenoxy lower alkynyl, wherein the phenyl moiety is unsubstituted, or a glucose oligomer thereof.Type: GrantFiled: February 5, 1990Date of Patent: October 26, 1993Assignee: Nippon Shinyaku Co. Ltd.Inventors: Yohji Ezure, Shigeaki Maruo, Hiroshi Yamashita, Katsunori Miyazaki, Makoto Sugiyama
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Patent number: 5254677Abstract: The present invention provides compounds of the general formula: ##STR1## wherein R is a trifluoromethyl radical or a cyano group. The present invention also provides processes for the preparation of these compounds, as well as reagents containing them. The present invention is also concerned with the use of these compounds as .beta.-galactosidase substrates, especially in a CEDIA system.Type: GrantFiled: June 25, 1991Date of Patent: October 19, 1993Assignee: Boehringer Mannheim GmbHInventors: Hans-Joachim Guder, Ruppert Herrmann, Dietmar Zdunek
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Patent number: 5254676Abstract: Heptasaccharides containing bivalent receptor determinants for the influenza virus of human isolates which inhibit binding of the virus to host cells as well as other saccharides used in preparation of the heptasaccharides are provided.Type: GrantFiled: February 19, 1993Date of Patent: October 19, 1993Assignee: E. I. du Pont de Nemours and CompanyInventor: Subramaniam Sabesan
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Patent number: 5252722Abstract: 5-O-.alpha.-D-Glucopyranosyl-L-ascorbic acid is a novel derivative of L-ascorbic acid which has a direct reducing activity and a satisfiable stability, as well as being readily hydrolyzed in vivo to form L-ascorbic acid. The 5-O-.alpha.-D-glucopyranosyl-L-ascorbic acid is prepared by allowing a saccharide-transferring enzyme together with or without .alpha.-glucosidase to a solution containing an .alpha.-glucosyl saccharide and L-ascorbic acid, and suitably used in food products, agents of anti-susceptive diseases and cosmetics containing thereof.Type: GrantFiled: October 21, 1992Date of Patent: October 12, 1993Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Takahiko Mandai, Masaru Yoneyama, Shuzo Sakai
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Patent number: 5250422Abstract: Incubation of 13.beta.-hydroxy ivermectin aglycone with a species of Bacillus subtilis or the incubation of 13-deoxy ivermectin aglycone with a mixed culture of a species of Bacillus subtilis and of Streptomyces griseus results in the production of 13-.beta. ivermectin monoglucopyranoside as the major product and of 5-.beta. ivermectin monoglucopyranoside as the minor product.Type: GrantFiled: May 11, 1992Date of Patent: October 5, 1993Assignee: Merck & Co., Inc.Inventors: Brian R. Petuch, Edward S. Inamine, Shieh-Shung T. Chen, Raymond F. White, Byron H. Arison
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Patent number: 5246695Abstract: The invention relates to alkylglycoside sulfosuccinates of the general formula (1) defined herein, useful in cosmetic preparations.Type: GrantFiled: April 1, 1992Date of Patent: September 21, 1993Assignee: REWO Chemische Werke GmbHInventors: Mathias Hintz, Hans-Jurgen Kohle, Christl Moller, Thomas Salomon, Joachim Weigand
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Patent number: 5243035Abstract: Sialic acid- containing glycolipid derivatives represented by the following general formula, their production method, and the particulate carriers that contain as a constituent the sialic acid-containing glycolipid derivative(s). ##STR1## wherein represents the .alpha.- or the .beta.- linkage, R.sup.1, a hydrogen atom or an acetyl group,R.sup.2, a hydrogen atom, a lower alkyl group with 1-4 carbon atoms, an alkaline metal ion, or such,X, an oxygen or sulfur atom or a residue represented by the following formula (II) or (III),--O(CH.sub.2).sub.m NHCO-- (II)where m represents an integer from 1 to 10.--O(CH.sub.2).sub.m CONH-- (III)Y, the formula (IV). ##STR2## where A represents a linear or branched chain acylamino group with 10-40 carbon atoms, or such B, a hydrogen atom, a carboxyl group or such, or the formula (V), and ##STR3## n, an integer of 0 to 3.Type: GrantFiled: August 15, 1991Date of Patent: September 7, 1993Assignee: Drug Delivery System Institute, Ltd.Inventors: Satoru Nakabayashi, Kunio Higashi, Shiro Miyoshi, Hitoshi Yamauchi
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Patent number: 5243036Abstract: A process for obtaining polymers with antiviral activity is disclosed. Molecules carrying carbonyl groups and hydroxyl groups in the .alpha. position relative to the carbonyl, and molecules carrying amino and sulfonated groups, react in water or in mixtures of water with water-miscible solvents. The reaction takes place under specific conditions of temperature, time and pH. The first reacting molecule may be a monosaccharide whereas the second reacting molecule may comprise aliphatic aminosulfates. Upon completion of the reaction the polymeric material is purified by conventional processes such that the polymeric material has a molecular weight between 1,500 and 100,000. The polymeric material has antiviral activity, either lacks or has very low toxicity and anticoagulant activity, and is thus suitable for pharmaceutical preparations for use in topical or systemic antiviral therapy in humans or animals.Type: GrantFiled: December 13, 1991Date of Patent: September 7, 1993Assignee: Laboratorios Andromaco S.A.Inventors: Juan Pablo Pivel Ranieri, Antonio F. Guerrero Gomez-Pamo, Luis Carrasco Llamas, Ma Jesus Almel Armendariz, Juan Antonio Leal Ojeda, Carmen Guerrero Benito
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Patent number: 5241059Abstract: Cyclodextrin derivatives having at least one group selected from among a sulfonic acid group, a sulfonic acid salt group, an ammonium salt group, a phosphoric acid group, a carboxyl group, a carboxylic acid salt group and a hydroxyl group are disclosed. The present invention provides cyclodextrin derivatives having extremely high solubility in water.Type: GrantFiled: December 6, 1991Date of Patent: August 31, 1993Assignee: Toppan Printing Co., Ltd.Inventor: Masanobu Yoshinaga
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Patent number: 5240914Abstract: The metabolism of tumor cells which have .beta.-glucuronidase activity is inhibited by administering to a patient a conjugate of glucuronic acid and a thiocarbamate compound of the formula: ##STR1## wherein R.sup.1 and R.sup.2 are selected from the group consisting of C.sub.1 -C.sub.6 alkyl and C.sub.1 -C.sub.6 cycloaliphatic, and M is selected from the group consisting of hydrogen, an electropositive, ionically bonded metal, and the radical ##STR2## and R.sup.3 and R.sup.4 are selected from the group consisting of C.sub.1 -C.sub.6 alkyl and C.sub.1 -C.sub.6 cycloaliphatic groups.Type: GrantFiled: December 22, 1992Date of Patent: August 31, 1993Assignees: Adolph Schwimmer, David RubinInventor: David Rubin
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Patent number: 5238597Abstract: Sucrosetricarboxylic acid can be prepared by oxidizing sucrose with oxygen, if desired in a mixture with inert gases, by means of a more effective catalyst than platinum/alumina.The product can be used in washing agents or as a food additive.Type: GrantFiled: February 25, 1991Date of Patent: August 24, 1993Assignee: Hoechst AktiengesellschaftInventors: Wolfram Fritsche-Lang, Ernst I. Leupold, Merten Schlingmann
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Patent number: 5236909Abstract: Octyl ethers and octadienyl ethers of glucose, sucrose, are disclosed. Compositions comprised of a mixture of octyl ethers of glucose and sucrose and those containing octadienyl ethers of sugar acids are also disclosed.Type: GrantFiled: March 26, 1991Date of Patent: August 17, 1993Assignees: Henkel Research Corporation, Zucker-Aktiengesellschaft Uelzen-BraunschweigInventors: Bert Gruber, Kenneth J. Weese, Hans-Peter Mueller, Karlheinz Hill, Arno Behr, James R. Tucker
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Patent number: 5233032Abstract: Polygalactomannans containing a hydroxybutyl ether substituent and a hydrophobic substituent can be used in the fracturing of subterranean formations.Type: GrantFiled: June 29, 1990Date of Patent: August 3, 1993Assignee: Stein, Hall & Co., Inc.Inventors: George M. Zody, Michael E. Morgan
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Patent number: 5229373Abstract: An agent for prevention or prophylaxis of general toxic effects or chronic neurotoxic effects which are caused by the administration of antineoplastic agents, which is to be given to patients prior to administration of said antineoplastic agents and which comprises as an active ingredient a mixture of gangliosides GM.sub.1, GD.sub.1a, GD.sub.1b and GT.sub.1b.Type: GrantFiled: June 25, 1987Date of Patent: July 20, 1993Assignee: Fidia S.p.A.Inventor: Francesco della Valle
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Patent number: 5225402Abstract: Novel carbostyril derivatives of the formula: ##STR1## wherein R.sup.1 is H, NO.sub.2, alkoxy, alkoxycarbonyl, alkyl, halogen, optionally substituted amino, OH, CN, COOH, alkanoyloxy, hydrazinocarbonyl; q is 1 to 3, and R is a group of the formula ##STR2## [wherein R.sup.2 is H, alkoxycarbonyl, optionally substituted phenoxycarbonyl, phenylalkenyl-CO-, optionally substituted phenylalkanoyl, alkanoyl, alkenyl-CO-, optionally substituted phenyl--SO.sub.2 --, --CONR.sup.8 R.sup.9, optionally substituted heterocyclic group--CO--, naphthyl--CO--, thienylalkanoyl, tricyclo[3.3.1.1]alkanoyl, ##STR3## (R.sup.13 is OH, optionally substituted alkoxy, --NR.sup.32 R.sup.33, --O--A--(E).sub.l --NR.sup.4 R.sup.5, --(B).sub.l 'NR.sup.6 R.sup.7, etc.), n is 1 or 2, m is 0 or 1 to 3, R.sup.3 is alkyl, R.sup.10 is --(CO).sub.l --NR.sup.11 R.sup.Type: GrantFiled: September 18, 1991Date of Patent: July 6, 1993Assignee: Otsuka Pharmaceutical Co., Ltd.Inventors: Hidenori Ogawa, Hisashi Miyamoto, Kazumi Kondo, Hiroshi Yamashita, Kenji Nakaya, Michiaki Tominaga, Yoichi Yabuuchi
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Patent number: 5223411Abstract: The present invention relates to non ionic surfactants having the general formula (I): ##STR1## wherein: R.sup.1 is H or CH3R.sup.2 is H or OHR.sup.3 is H or OHR.sup.4 is an alkyl of formula --(CH.sub.2).sub.n --CH.sub.3 wherein n is an integer comprised between 6 and 20It also relates to a process for treatment of proteins containing medium without denaturation of said proteins, using these surfactants.Type: GrantFiled: May 14, 1990Date of Patent: June 29, 1993Assignee: Centre National de la Recherche Scientifique (CNRS)Inventors: Daniel Plusquellec, Henri Wroblewski
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Patent number: 5221735Abstract: Inclusion complexes of the polyenes apocarotenal and lycopene in a cyclodextrin as well as their manufacture are described. The inclusion complexes are soluble in water, alcohol or water/alcohol mixtures.Type: GrantFiled: February 14, 1992Date of Patent: June 22, 1993Assignee: Hoffmann-La Roche Inc.Inventors: Bruno Leuenberger, Hansjorg Stoller
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Patent number: 5220008Abstract: Heptasaccharides containing bivalent receptor determinants for the influenza virus of human isolates which inhibit binding of the virus to host cells as well as other saccharides used in preparation of the heptasaccharides are provided.Type: GrantFiled: November 22, 1991Date of Patent: June 15, 1993Assignee: E. I. du Pont de Nemours and CompanyInventor: Subramaniam Sabesan
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Patent number: 5220005Abstract: Dioxetane compounds reactable with an enzyme to release optically detectable eneregy are disclosed. These compounds have the formula: ##STR1## wherein T is a carboxylic acid or methoxy substituted adamantyl group bound to the ring through a spiro bond, Y is phenoxy or napthyloxy and X is methoxy, ethoxy or propoxy. Z is an enzyme cleavable group such that when cleaved by an enzyme, an electron rich moiety is left bound to the dioxetane ring.Type: GrantFiled: August 30, 1990Date of Patent: June 15, 1993Assignee: Tropix, Inc.Inventor: Irena Y. Bronstein
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Patent number: 5218098Abstract: 5-(.alpha.-D-glucopyranosyloxymethyl)-furan-2-carboxaldehyde (GMF) is produced from isomaltulose by heating a solution of isomaltulose in a strongly polar aprotic solvent in the presence of an acidic ion-exchanger, either batchwise or by percolation of the solution through a column filled with the ion-exchanger. Novel derivatives of GMF of the formula (I) ##STR1## wherein R' is a hydrogen atom, an acyl group or an alkyl group and X represents --CH.sub.2 OH, --COOH, --C(H).dbd.NOH, --CN, --CH.sub.2 NHR" or --CH.dbd.CR"R'", where R" is a hydrogen atom, an acyl or aryl group or an alkyl group with up to 20 C atoms and R'" is a hydrogen atom, --NO.sub.2, --CN, --COOalkyl or acyl are described, and also their preparation and use for the preparation of surface-active compounds.Type: GrantFiled: October 31, 1990Date of Patent: June 8, 1993Assignee: Sudzucker AG Mannheim/OchsenfurtInventors: Frieder W. Lichtenthaler, Dierk Martin, Thomas Weber, Hubert Schiweck
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Patent number: 5218096Abstract: Lactoneotrehalose, a novel saccharide shown by the formula O-.beta.-D-galactopyranosyl-(1.fwdarw.4)-O-.beta.-D-glucopyranosyl .alpha.-D-glucopyranoside, is prepared by allowing a saccharide-transferring enzyme to act on an aqueous solution containing lactose and amylaceous substance. Lactoneotrehalose is a non-reducing oligosaccharide, extremely stable, readily soluble in water, and substantially free of hygroscopicity, as well as having a satisfiable quality and moderate sweetness. These render lactoneotrehalose very useful in the preparations of orally-administrable products, cosmetics and pharmaceuticals.Type: GrantFiled: October 7, 1991Date of Patent: June 8, 1993Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Takashi Shibuya, Hiroto Chaen, Shuzo Sakai
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Patent number: 5212292Abstract: A process for the preparation of alkyl polyglycosides containing an alkyl group of about 8 to 24 carbon atoms, which comprises glycosylating and transglycosylating one or more saccharides, wherein the glycosylation is carried out in an evaporator at an acid number of about 1 to 10 mg of KOH/g.Type: GrantFiled: November 15, 1991Date of Patent: May 18, 1993Assignee: Huels Aktiengesellschaft-PB 15Inventor: Norbert Ripke
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Patent number: 5212076Abstract: The present invention provides a dye other than red and purple which is obtained from cultured cells of Euphorbia milli. The present invention also provides cultured cells containing quercetin glucuronide in a large amount, derived from tissues or cells of Euphorbia milli.Type: GrantFiled: September 16, 1991Date of Patent: May 18, 1993Assignee: Nippon Paint Co., Ltd.Inventors: Yoshikazu Yamamoto, Yasuhiro Kinoshita
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Patent number: 5210077Abstract: Antibodies (polyclonal and monoclonal) having specificity for cytokinins having a glycosylated isoprenoid side chain are described. The antibodies simultaneously recognize a purine ring, an isoprenoid side chain, and a 4'-O-glycoside. The antibodies were elicited using a novel hapten, 9-(2-carboxyethyl) cytokinin-O-glycoside. Immunoassay methods for the determination of cytokinins having a glycosylated isoprenoid side chain which utilize the antibodies are also described.Type: GrantFiled: April 6, 1989Date of Patent: May 11, 1993Assignee: The United States of America as represented by the Secretary of AgricultureInventors: David L. Brandon, Joseph W. Corse
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Patent number: 5208148Abstract: The claimed invention relates to a substrate for evaluating glycosidic enzymes comprising a fluorescein derivative of the general formula: ##STR1## wherein GlyX is a carbohydrate bonded to fluorescein by a glycosidic linkage;Y, which may be the same as GlyX or different, is an alkyl ether, an ester, or a glycosidically linked carbohydrate;R is a lipophilic residue containing from 1 to 21 carbon atoms; andL links the R residue to fluorescein.A preferred embodiment of the invention is a non-fluorescent substrate specifically hydrolyzable by a glycosidase inside a cell to yield, after greater than about 2 minutes, a fluorescent detection product excitable at between about 460 nm and 550 nm and with fluorescence observable at an emission wavelength longer than the excitation wavelength, which fluorescent detection product is retained inside a viable cell more than about 2 hours at greater than about 15.degree. C. and which is non-toxic to the cell.Type: GrantFiled: December 7, 1990Date of Patent: May 4, 1993Assignee: Molecular Probes, Inc.Inventors: Richard P. Haugland, John J. Naleway, Yu-zhong Zhang
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Patent number: 5206356Abstract: Synthetic glycosaminosyl transferase-V acceptors and inhibitors are disclosed. Minimal trisaccharide inhibitors and acceptors specific for GnT-V, which is associated with the ability of cells to metastasize are useful, in diagnosis and treatment of conditions characterized by metastatic cells.Type: GrantFiled: July 15, 1991Date of Patent: April 27, 1993Assignee: Chembiomed, Ltd.Inventors: James M. Pierce, Ole Hindsgaul
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Patent number: 5206355Abstract: Sucrose is treated with an acid catalyst to form an amorphous sucrose anhydrous melt. Heating of the anhydrous melt at 80.degree.-100.degree. C. forms three known kestoses and the novel products, viz. .alpha.-fructosylsucrose anomers of 6-kestose, neokestose, and 1-kestose. Heating of the anhydrous melt at 125.degree.-175.degree. C. produces a fructoglucan sucrose polymer having a molecular weight of about 2,000 to 10,000 Daltons. All of these products are useful as sweeteners, food additives, additives for animal food, and bulking agents.Type: GrantFiled: March 19, 1992Date of Patent: April 27, 1993Assignee: The University of MontanaInventors: Geoffrey N. Richards, Merilyn Manley-Harris
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Patent number: 5202357Abstract: A composition suitable for topical application to human skin comprises, in addition to a suitable vehicle an active ingredient which can control skin barrier functions. The active ingredient is (a) a long chain .omega.-hydroxy fatty acid or a carboxy-substituted derivative, (b) an hydroxy- or epoxy-derivative of an essential fatty acid or an ester formed between (a) and (b). Certain novel compounds of structures (a), (b) and (a)(b) esters are also claimed.Type: GrantFiled: June 21, 1990Date of Patent: April 13, 1993Assignee: Lever Brothers Company, Division of Conopco, Inc.Inventors: Paul A. Bowser, Albert Froling, Lammert Heslinga, Udo M. T. Houtsmuller, Diederik H. Nugteren, Hendrik J. J. Pabon, Colin Prottey
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Patent number: 5200516Abstract: There are disclosed processes for the synthesis of substituted and unsubstituted arene diols useful in the further synthesis of sugars, sugar derivatives, chiral synthons or amino acids.Type: GrantFiled: December 6, 1991Date of Patent: April 6, 1993Assignee: Virginia Tech Intellectual PropertiesInventor: Tomas Hudlicky
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Patent number: 5191073Abstract: Chromogenic merocyanine enzyme substrate compounds of the general formula: ##STR1## where Y is an enzymatically-cleavable group such as a radical of a sugar, carboxylic acid, amino acid, peptide, phosphoric acid, or sulfuric acid; A and B represent residues that complete 5- or 6-membered ring systems; R.sup.1 is substituted or unsubstituted alkyl; R.sup.2 and R.sup.3, independently, are hydrogen or lower alkyl; m, n, and p, which can be different, are integers from 0 through 3 provided that m+n+p must be at least 2; and X is an appropriate counterion (anion).Type: GrantFiled: February 26, 1992Date of Patent: March 2, 1993Assignee: Miles Inc.Inventors: Paul F. Corey, M. Teresa Yip
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Patent number: 5191071Abstract: Compounds of the formula (R-COO).sub.n X-OR.sup.1, wherein R.sup.1 is optionally substituted alkyl, phenyl, or alkyl phenyl, n is 1, 2 or 3, X is a carbohydrate moiety, and R is optionally substituted alkyl, have superior effects as additives in detergents. These compounds can be prepared by esterification of glycosides using specific enzymes.Type: GrantFiled: June 28, 1991Date of Patent: March 2, 1993Assignee: Novo Nordisk A/SInventors: Ole Kirk, Frederik Bjorkling, Sven E. Godtfredsen
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Patent number: 5185327Abstract: A new glucan derivative having at least one side chain branch of a compound selected from the group consisting of galactopyranose, L-arabinofuranose and oligomers thereof is disclosed. These glucan derivatives have high tumoricidal activity and are usable as a tumoricide.Type: GrantFiled: August 24, 1990Date of Patent: February 9, 1993Assignee: Ajinomoto Company, Inc.Inventors: Kei Matsuzaki, Iwao Yamamoto, Akira Misaki, Yoshiaki Sone
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Patent number: 5185436Abstract: Compounds which are hydrolyzed in vivo to n-butyric acid are esters of butyric acid in which the alcohol group is selected from the group consisting of polyhydric alcohols, cyclic and acyclic aldose or ketose monosaccharides and their acetonides. Of particular interest are O-butanoyl-6-O-ispropylidene-1,2,.alpha. O-glucofuranose, tri-O-butanoyl 3, 5, 6,- isopropylidene-1,2 .alpha.-D-glucofuranose, O-butanoyl-6-di-O-isopropylidene-1,2 : 3,4 .alpha.-D-galactopyranose, and penta-O-butanoyl-1,2,3,4,6 D-galactopyranose and penta-O-butanoyl-1,2,3,4,6.alpha. D-galactopyranose, )-n-butanoyl-1-isopropylidene-2,3 glyceryl, O-butanoyl-3-di-o-palmitoyl-1,2 glyceryl, and di-O-n butanoyl-2,3 O-palmitoyl-1 glyceryl.Type: GrantFiled: March 29, 1990Date of Patent: February 9, 1993Assignee: Association Pour La Recherche Therapeutique Anti-CancereuseInventors: Pierre J. Villa, Francois Pieri, Ginolino Ronco, Emile P. R. Segard
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Patent number: 5182378Abstract: A sulfate derivative of galactan extracted from Klebsiella mainly comprised of sulfate neutral oses in a proportion of 20 to 90% of hydroxyls and their preparation and method of use.Type: GrantFiled: December 4, 1990Date of Patent: January 26, 1993Assignee: Roussel UclafInventors: Pierre Smets, Rene Zalisz
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Patent number: 5182373Abstract: The present invention provides quaternary ammonium-substituted saponin ethers having improved electrostatic and hydrophobic interactive activities, and therefore very useful in hair-care and skin-care products. The product of the invention, particularly, eliminates efficiently the static flyaway of hair and gives excellent conditioning effect to hair when used in hair-care products. The quaternary ammonium-substituted saponin ethers can be prepared by reacting a soya or ginseng saponin or an aglycone thereof with a cationic compound selected from quaternary ammonium halohydrin or quaternary ammonium epoxide.Type: GrantFiled: May 30, 1990Date of Patent: January 26, 1993Assignee: Pacific Chemical Co., Ltd.Inventors: Young D. Kim, Byung J. Ha
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Patent number: 5179201Abstract: Alkyl (poly)glucoside ether carboxylates of the formula ##STR1## where R is C.sub.1 - to C.sub.24 -alkyl, C.sub.1 - to C.sub.24 -alkylphenyl, hydroxy-C.sub.2 - to C.sub.6 -alkyl, hydroxy-C.sub.1 - to C.sub.24 -alkylphenyl, ##STR2## or H, R.sup.1 is H or methyl,R.sup.2 is ##STR3## X is a hydrogen, alkali metal, ammonium and/or substituted ammonium equivalent,x is from 1 to 10, y is from 0 to 10, z is from 1 to 4,a process for the preparation of the compounds of the formula I and the use of these compounds as an additive to phosphate-free and low-phosphate detergents, are described.Type: GrantFiled: May 3, 1991Date of Patent: January 12, 1993Assignee: BASF AktiengesellschaftInventors: Alfred Oftring, Elisabeth Kappes, Richard Baur, Alexander Kud
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Patent number: 5177198Abstract: A process of making oligoribonucleoside and deoxyribonucleoside boranophosphates and salts thereof is disclosed. The process comprises the steps of condensing a ribonucleotide or deoxyribonucleotide with a nucleoside phosphitylating agent, wherein the phosphitylating agent includes a 5' -OH group protected by an acid-labile protecting group and a moiety bonded to the phosphorus atom therein that is a stronger proton acceptor than the 5'-OH group of the ribonucleotide or deoxyribonucleotide to form a reaction intermediate, then oxidizing the reaction intermediate so formed with a compound comprising a Lewis base and a boron-containing substituent, wherein the Lewis base is a weaker electron donor than the phosphite phosphorus of the reaction intermediate, to form a ribonucleotide or deoxyribonucleotide boranophosphate. These steps are repeated on the nucleotide boranophosphate to form an oligonucleotide boranophosphate.Type: GrantFiled: November 30, 1989Date of Patent: January 5, 1993Assignees: University of N.C. at Chapel Hill, Duke University, Boron Biologicals, Inc.Inventors: Bernard F. Spielvogel, Anup Sood, Iris H. Hall, Barbara Ramsay Shaw
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Patent number: 5175277Abstract: A rapidly hydrating welan gum useful for preparing cementitious products. The gum can be dry blended with cement or other dry component and then added to a preformed cement/water pre-mix, thereby alleviating the need for hydrating the gum prior to addition to a cement/water mixture.Type: GrantFiled: March 20, 1991Date of Patent: December 29, 1992Assignee: Merck & Co., Inc.Inventors: Walter G. Rakitsky, Danny D. Richey
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Patent number: 5175257Abstract: Chelating compounds of specified N.sub.2 S.sub.2 N.sub.3 S derived structure are useful for radiolabeling targeting molecules such as antibodies. Cleavable ester orthioester linkers connect the radionuclide metal chelates to the antibodies. The radiolabeled antibodies have improved biodistribution properties, including reduced localization within the intestines and kidneys.Type: GrantFiled: January 14, 1991Date of Patent: December 29, 1992Assignee: NeoRx CorporationInventors: Sudhakar Kasina, Ananthachari Srinivasan, John M. Reno, Linda M. Gustavson, Jeffrey N. Fitzner, David S. Jones
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Patent number: 5169636Abstract: The invention provides liposomes characterized in that the lipid membrane thereof contains a glycolipid resulting from O-glycoside or S-glycoside bonding of a lipid and at least one carbohydrate selected from the group consisting of uronic acids, uronosyl-oligosaccharides, oligosaccharides composed of glucosamine and glucuronic acid, oligosaccharides composed of galactosamine and glucuronic acid, oligosaccharides composed of glucuronic acid and xylose, oligosaccharides composed of glucuronic acid and glucose, oligosaccharides composed of glucuronic acid and galactose, oligosaccharides composed of glucuronic acid and mannose, oligosaccharides composed of galacturonic acid and rhamnose, ketoaldonic acids, 2-acetamido-2-deoxyuronic acids, 2-acetamido-2-deoxyuronic acid-containing oligosaccharides, 6-O-carboxyethyl-.beta.-D-glucose, 6-O-carboxymethyl-.beta.-D-glucose, 6'-O-carboxymethyl-.beta.-D-maltose and 6'-O-carboxyethyl-.beta.-D-maltose.Type: GrantFiled: November 14, 1989Date of Patent: December 8, 1992Assignee: Nippon Fine Chemical Co., Ltd.Inventors: Yukihiro Nanba, Toshiyuki Sakakibara, Naoto Oku