Patents Issued in November 24, 2016
  • ERK-DERIVED PEPTIDES AND USES THEREOF
    Publication number: 20160340655
    Abstract: An isolated peptide being no longer than 20 amino acids comprising a sequence at least 95% homologous to the sequence GQLNHILGILGX1PX2QED (SEQ ID NO: 4), wherein X1 and X2 are any amino acid, the peptide being capable of preventing extracellular signal-regulated kinase1/2 (ERK) translocation into the nucleus.
    Type: Application
    Filed: September 15, 2014
    Publication date: November 24, 2016
    Inventors: Rony SEGER, Alexander PLOTNIKOV
  • PEPTIDES DERIVED FROM GSE 24.2 FOR TREATING DISEASES CAUSED BY OXIDATIVE STRESS AND DAMAGE TO DNA
    Publication number: 20160340656
    Abstract: This invention relates to a polypeptide which comprises the fragment SEQ ID No. 2 of peptide GSE24.2 (SEQ ID No. 1) and can reduce the production of free radicals and/or damage to the DNA structure of a cell, optionally comprising nuclear localisation sequences. The invention also relates to a polynucleotide and to a vector, which comprise sequences coding for said polypeptide, and to a pharmaceutical composition comprising said polypeptide, polynucleotide and/or vector. The invention further relates to the applications of the polypeptide such as the use thereof for treating and/or preventing diseases caused by an increase in oxidative stress and/or damage to cellular DNA, such as ataxia-telangiectasia or dyskeratosis congenita, or the use thereof in tissue engineering and cell culture for increasing the viability of same.
    Type: Application
    Filed: October 24, 2014
    Publication date: November 24, 2016
    Inventors: Rosario PERONA ABELLÓN, Leandro SASTRE GARZÓN, Laura PINTADO BERNINCHES, Jaime CARRILLO GARCÍA, Antonio MOLINA PACHÓN, Laura IRRADICCIO SILVA, Cristina MANGUAN GARCÍA
  • Mutant Reverse Transcriptase and Methods of Use
    Publication number: 20160340657
    Abstract: The invention relates to the generation and characterization of stable MMLV reverse transcriptase mutants. The invention also discloses methods of using stable MMLV reverse transcriptase mutants.
    Type: Application
    Filed: April 4, 2016
    Publication date: November 24, 2016
    Applicant: Agilent Technologies, Inc.
    Inventors: Holly H. Hogrefe, Bahram Arezi, Weimei Xing
  • PTERIS VITTATA PHYTASE NUCLEOTIDE AND AMINO ACID SEQUENCES AND METHODS OF USE
    Publication number: 20160340658
    Abstract: The present disclosure provides isolated and synthetic DNA and cDNA molecules encoding a phytase from the root of Pteris vittata (PV); root PV phytase proteins and peptides; root PV phytase antisense molecules, vectors, transgenic cells and plants containing root PV phytase nucleic acid molecules, isolated polypeptides, or antisense molecules; genetic markers for root PV phytase; and methods of using these nucleic acid or polypeptide molecules to improve phosphorus utilization from phytate by plants and animals.
    Type: Application
    Filed: November 7, 2014
    Publication date: November 24, 2016
    Inventors: JASON LESSL, LENA Q. MA, BALASUBRAMANI RATHINASABAPATHIR, CHARLES L. GUY
  • ACTIN BINDING PEPTIDES AND COMPOSITIONS COMPRISING SAME FOR INHIBITING ANGIOGENESIS AND TREATING MEDICAL CONDITIONS ASSOCIATED WITH SAME
    Publication number: 20160340659
    Abstract: The present invention, in some embodiments thereof, relates to biologically active peptides and, more particularly, but not exclusively, to peptides from T2 RNase (RNASET2) having actin binding, pharmaceutical compositions comprising the same, therapeutic use thereof and methods for their production.
    Type: Application
    Filed: January 29, 2015
    Publication date: November 24, 2016
    Inventors: Oded SHOSEYOV, Betty SCHWARTZ, Shani DORON, Liron NESIEL, Assaf FRIEDLER, Hadar AMARTELY, Levava ROIZ, Patricia SMIRNOFF, Iris LEWIN
  • CRISPR-CAS SYSTEMS, CRYSTAL STRUCTURE AND USES THEREOF
    Publication number: 20160340660
    Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.
    Type: Application
    Filed: June 2, 2016
    Publication date: November 24, 2016
    Inventors: Feng Zhang, Osamu NUREKI, Hiroshi NISHIMASU, Ryuichiro ISHITANI
  • DELIVERY, USE AND THERAPEUTIC APPLICATIONS OF THE CRISPR-CAS SYSTEMS AND COMPOSITIONS FOR GENOME EDITING
    Publication number: 20160340661
    Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
    Type: Application
    Filed: June 10, 2016
    Publication date: November 24, 2016
    Inventors: Le Cong, David Benjamin Turitz Cox, Matthias Heidenreich, Randall Jeffrey Platt, Lukasz Swiech, Feng Zhang
  • ENGINEERING OF SYSTEMS, METHODS AND OPTIMIZED GUIDE COMPOSITIONS FOR SEQUENCE MANIPULATION
    Publication number: 20160340662
    Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR-Cas system.
    Type: Application
    Filed: August 5, 2016
    Publication date: November 24, 2016
    Inventors: Feng Zhang, Le Cong, Patrick Hsu, Fei Ran
  • THERMOSTABLE GLYCOSIDE HYDROLASE
    Publication number: 20160340663
    Abstract: A thermostable glycoside hydrolase having a glycoside hydrolase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1; (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.0; (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolysis activity against a substrate of PSA and hydrolysis activity against a substrate of CMC at least under conditions of 65° C. and pH 4.
    Type: Application
    Filed: May 17, 2016
    Publication date: November 24, 2016
    Inventors: Migiwa SUDA, Jiro OKUMA, Asuka YAMAGUCHI, Yoshitsugu HIROSE, Yasuhiro KONDO, Masaru SATO, Daisuke SHIBATA
  • COMPOSITIONS AND METHODS COMPRISING A XYLANASE ENZYME VARIANT
    Publication number: 20160340664
    Abstract: The present invention provides hemicellulytic enzyme variants. Specifically, the present invention provides xylanase variants having one or more modifications as compared to a parent xylanase enzyme resulting in at least one improved property. In addition, the present invention provides compositions comprising a xylanase variant of the invention. The present invention also provides methods of degrading hemicellulotic material, including arabino-xylan using compositions comprising a xylanase variant of the invention.
    Type: Application
    Filed: January 30, 2015
    Publication date: November 24, 2016
    Inventors: Susan Arent Lund, Jacob F. Cramer, Igor Nikolaev, Pim Van Der Kley
  • Bacteria Engineered to Reduce Hyperphenylalaninemia
    Publication number: 20160340665
    Abstract: Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of modulating and treating diseases associated with hyperphenylalaninemia are disclosed.
    Type: Application
    Filed: May 13, 2016
    Publication date: November 24, 2016
    Inventors: Dean Falb, Vincent M. Isabella, Jonathan W. Kotula, Paul F. Miller
  • METHOD FOR THE BIOCATALYTIC CYCLIZATION OF TERPENES AND CYCLASE MUTANTS EMPLOYABLE THEREIN
    Publication number: 20160340666
    Abstract: The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
    Type: Application
    Filed: August 11, 2016
    Publication date: November 24, 2016
    Inventors: Michael Breuer, Bernhard Hauer, Dieter Jendrossek, Gabrielle Siedenburg, Juergen Pleiss, Demet Sirim, Silivia Fadenrecht
  • IMMOBILIZED PROTEINS AND USE THEREOF
    Publication number: 20160340667
    Abstract: The invention relates to an immobilized protein material comprising a protein that is immobilized on a glass material or organic polymer through affmity tag binding. The glass material may be a porous glass material such as (hybrid) controlled porosity glass. The invention also relates to the use of an immobilized enzyme material as a heterogeneous biocatalyst in chemical synthesis. The invention further relates to a method for the immobilization of affmity tagged proteins on a glass material or organic polymer, and to a method for the purification and isolation of affmity tagged proteins by the immobilization of such proteins on a glass material or organic polymer.
    Type: Application
    Filed: January 30, 2015
    Publication date: November 24, 2016
    Inventors: Karim Engelmark CASSIMJEE, Jan-Erling BÄCKVALL
  • SINGLE-STEP DNA PREPARATION FOR POLYMERASE CHAIN REACTION USING MAGNETIC CHITOSAN MICROPARTICLES
    Publication number: 20160340668
    Abstract: The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.
    Type: Application
    Filed: May 20, 2016
    Publication date: November 24, 2016
    Applicants: UNIVERSITY OF MARYLAND, CANON U.S. LIFE SCIENCES, INC.
    Inventors: IAN M. WHITE, SRINIVASA RAGHAVAN, KUNAL R. PANDIT, IMALY NANAYAKKARA, WEIDONG CAO
  • METHOD AND APPARATUS FOR ISOLATING NUCLEIC ACIDS
    Publication number: 20160340669
    Abstract: A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.
    Type: Application
    Filed: August 8, 2016
    Publication date: November 24, 2016
    Inventors: Lin Chen, BING Zhang, Klaus Hochleitner, Rong Hou, Yanju Wang
  • NOVEL OLIGO-LINKER-MEDIATED DNA ASSEMBLY METHOD AND APPLICATIONS THEREOF
    Publication number: 20160340670
    Abstract: A method for generating a library of expression vectors comprising a plurality of donor sequences and a plurality of oligo-linker nucleic acids, termed Oligonucleotide Linker-Mediated DNA Assembly (OLMA), is described. Also described are applications of the OLMA method, including the simultaneous tuning of several factors in metabolic and biological pathways, and the combinatorial high throughput optimization of metabolic and biological pathways.
    Type: Application
    Filed: May 20, 2016
    Publication date: November 24, 2016
    Inventors: Chunbo LOU, Yong TAO, Xuejin ZHAO, Shasha ZHANG, Lihua ZHANG, Chunhua ZHAI, Zhenyu LIU
  • Clonal Analysis of Functional Genomic Assays and Compositions for Practicing Same
    Publication number: 20160340671
    Abstract: Methods of clonal analysis of functional genomic assays are provided. Aspects of the invention include transducing a population of target cells with a packaged viral effector library made up of a plurality of effector construct subsets, wherein each effector construct subset of the library includes a plurality of effector constructs having a common effector cassette linked to a distinct clonal barcode. Inclusion of distinct clonal barcodes in the effector construct subset allows for determination of the clonal representation of an effector construct subset in transduced target cells that exhibit a specific phenotype. Aspects of the invention further include compositions, e.g., libraries and components thereof, which find use in practicing the methods.
    Type: Application
    Filed: June 6, 2016
    Publication date: November 24, 2016
    Inventors: Alex Chenchik, Donato Tedesco, Mikhail Makhanov
  • DE NOVO SYNTHESIZED GENE LIBRARIES
    Publication number: 20160340672
    Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes.
    Type: Application
    Filed: August 10, 2016
    Publication date: November 24, 2016
    Inventors: William BANYAI, Bill James PECK, Andres FERNANDEZ, Siyuan CHEN, Pierre INDERMUHLE
  • METHODS FOR THE PREVENTION AND THE TREATMENT OF EXTRACAPILLARY GLOMERULONEPHRITIS
    Publication number: 20160340673
    Abstract: The present invention relates to the prevention and the treatment of extracapillary glomerulonephritis such as rapidly progressive glomerulonephritis and collapsing glomerulonephritis.
    Type: Application
    Filed: January 22, 2015
    Publication date: November 24, 2016
    Inventors: Pierre-Louis Tharaux, Carole Henique-Greciet
  • Compositions and Methods to Inhibit EZH2 for the Treatment of Cardiovascular Diseases
    Publication number: 20160340674
    Abstract: The present invention relates to compositions and methods for treatment and/or prevention of a cardiovascular disease. In one embodiment, the invention provides compositions and methods for decreasing one or more of the level, production, and activity of EZH2.
    Type: Application
    Filed: February 9, 2015
    Publication date: November 24, 2016
    Inventor: Zheng-Gen JIN
  • MICRORNA MODULATION OF ARTHEROSCLEROSIS AND USES THEREOF
    Publication number: 20160340675
    Abstract: The presently disclosed subject matter provides anti-miRNAs for the treatment of diseases associated with atherogenesis and methods of treatment, and diagnosis and/or prognosis of these diseases using lipoprotein-associated miRNAs.
    Type: Application
    Filed: May 23, 2016
    Publication date: November 24, 2016
    Inventors: Kasey Vickers, Carrie Wiese, Valentina Kon, Zhi-Qi Xu
  • METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA
    Publication number: 20160340676
    Abstract: The invention provides compositions and methods for selectively reducing the expression of a gene product from a desired target gene, as well as treating diseases caused by expression of the gene. The method involves introducing into the environment of a cell an amount of a double-stranded RNA (dsRNA) such that a sufficient portion of the dsRNA can enter the cytoplasm of the cell to cause a reduction in the expression of the target gene. The dsRNA has a first oligonucleotide sequence that is between 26 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of from about 19 to about 23 nucleotides is complementary to a nucleotide sequence of the RNA produced from the target gene.
    Type: Application
    Filed: June 13, 2016
    Publication date: November 24, 2016
    Applicants: CITY OF HOPE, INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: John J. ROSSI, Mark A. BEHLKE, Dongho KIM
  • ANTIVIRAL THERAPY
    Publication number: 20160340677
    Abstract: The present invention provides a method of identifying host cell molecules which may be modulated to inhibit viral replication and method of testing antiviral compounds. In addition, the invention provides compositions, methods and medicaments for treating viral infections and/or diseases or conditions caused or contributed to by viruses.
    Type: Application
    Filed: August 25, 2014
    Publication date: November 24, 2016
    Inventor: Amy Buck
  • TREATMENT OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO VEGF
    Publication number: 20160340678
    Abstract: Oligonucleotide compounds modulate expression and/or function of Vascular Endothelial Growth Factor (VEGF) polynucleotides and encoded products thereof. Methods for treating diseases associated with Vascular Endothelial Growth Factor (VEGF) comprise administering one or more Oligonucleotide compounds designed to inhibit the VEGF natural antisense transcript to patients.
    Type: Application
    Filed: August 5, 2016
    Publication date: November 24, 2016
    Applicant: CuRNA, Inc.
    Inventors: Joseph COLLARD, Olga KHORKOVA SHERMAN
  • KETOHEXOKINASE (KHK) iRNA COMPOSITIONS AND METHODS OF USE THEREOF
    Publication number: 20160340679
    Abstract: The present invention relates to RNAi agents, e.g., double-stranded RNAi agents, targeting the ketohexokinase (KHK) gene, and methods of using such RNAi agents to inhibit expression of KHK and methods of treating subjects having a KHK-associated disorder, e.g., liver disease (e.g., fatty liver, steatohepatitis), dyslipidemia (e.g., hyperlipidemia, high LDL cholesterol, low HDL cholesterol, hypertriglyceridemia, postprandial hypertriglyceridemia), disorders of glycemic control (e.g., insulin resistance, diabetes), cardiovascular disease (e.g., hypertension, endothelial cell dysfunction), kidney disease (e.g., acute kidney disorder, tubular dysfunction, proinflammatory changes to the proximal tubules), metabolic syndrome, adipocyte dysfunction, visceral adipose deposition, obesity, hyperuricemia, gout, eating disorders, and excessive sugar craving.
    Type: Application
    Filed: August 1, 2016
    Publication date: November 24, 2016
    Inventors: Kevin Fitzgerald, Brian Bettencourt, Gregory Hinkle, Jennifer Willoughby
  • MODULATION OF KCNH2 ISOFORM EXPRESSION BY OLIGONUCLEOTIDES AS A THERAPEUTIC APPROACH FOR LONG QT SYNDROME
    Publication number: 20160340680
    Abstract: Oligonucleotides with activity in preventing poly(A) adenylation at intron 9 of the KCNH2 gene, as well as pharmaceutical compositions comprising the oligonucleotides and methods of using the oligonucleotides to treat long QT syndrome in a subject are disclosed. The oligonucleotides include antisense sequences corresponding to sites termed DSE-1 and DSE-2 in intron 9.
    Type: Application
    Filed: August 8, 2016
    Publication date: November 24, 2016
    Applicant: OREGON HEALTH & SCIENCE UNIVERSITY
    Inventors: Zhengfeng ZHOU, Qiuming GONG, Matthew STUMP
  • Method for Producing an L-Amino Acid Using a Bacterium of the Family Enterobacteriaceae Having Overexpressed the yajL Gene
    Publication number: 20160340681
    Abstract: The present invention provides a method for producing L-amino acids or salts thereof by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to overexpress the yajL gene.
    Type: Application
    Filed: August 10, 2016
    Publication date: November 24, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Natalia Viktorovna Stoynova, Vera Georgievna Doroshenko
  • METHOD OF OBTAINING TRANSFORMED PLANT CELL
    Publication number: 20160340682
    Abstract: The present invention relates to a method of obtaining a transformed plant cell. The present invention comprises the steps of: (a) co-transforming an intended DNA and a first marker gene into a plant cell; and (b) selecting from the transformed cells obtained in the step (a), a transformed plant cell wherein the intended DNA is introduced into a chromosome thereof, and the first marker gene is not introduced, wherein the method does not contain a step to exclude a transformed cell with only the intended DNA introduced into the chromosome by positive selection using the first marker gene.
    Type: Application
    Filed: February 4, 2015
    Publication date: November 24, 2016
    Applicant: JAPAN TOBACCO INC.
    Inventors: Yukoh HIEI, Toshihiko KOMARI
  • NANOCARRIER BASED PLANT TRANSFECTION AND TRANSDUCTION
    Publication number: 20160340683
    Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
    Type: Application
    Filed: June 2, 2016
    Publication date: November 24, 2016
    Inventors: Francois EUDES, Archana CHUGH, Priti MAHESHWARI
  • DISCOVERY AND UTILIZATION OF SORGHUM GENES (MA5/MA6)
    Publication number: 20160340684
    Abstract: Methods and composition for the production of non-flowering or late flowering sorghum hybrid. For example, in certain aspects methods for use of molecular markers that constitute the Ma5/Ma6 pathway to modulate photoperiod sensitivity are described. The invention allows the production of plants having improved productivity and biomass generation.
    Type: Application
    Filed: October 2, 2012
    Publication date: November 24, 2016
    Applicant: The Texas A&M University System
    Inventors: John E. Mullet, William L. Rooney, Patricia E. Klein, Daryl Morishige, Rebecca Murphy, Jeff A. Brady
  • SORGHUM-DERIVED TRANSCRIPTION REGULATORY ELEMENTS PREDOMINANTLY ACTIVE IN ROOT HAIR CELLS AND USES THEREOF
    Publication number: 20160340685
    Abstract: Transcription regulatory elements, namely promoter and terminator sequences, obtained from Sorghum bicolor that drive RNA transcription predominately in root hair cells are described, as well as cassettes, expression vectors, and genetically modified plants containing these transcription regulatory elements. The genetically modified plants can be gymnosperms, dicots, or monocots. Methods of directing transcription of a heterologous polynucleotide under control of these transcription regulatory elements in a genetically modified plant's root hair cells are also provided.
    Type: Application
    Filed: May 20, 2015
    Publication date: November 24, 2016
    Inventors: Scott R. Baerson, Zhiqiang Pan, James J. Polashock
  • Improved Digestibility of Plant Biomass
    Publication number: 20160340686
    Abstract: Plants described herein have increased biomass and are more readily digested into fermentable sugars when the plants express increased levels of one or more types of CGR2 and/or CGR3 enzymes.
    Type: Application
    Filed: June 27, 2014
    Publication date: November 24, 2016
    Inventors: Federica Brandizzi, Curtis Wilkerson, Sang Jin Kim, Michael Held
  • METHOD FOR PRODUCTION OF TRANSGENIC COTTON PLANTS
    Publication number: 20160340687
    Abstract: The present invention provides a method for producing transgenic Cotton plants. In one method transformed plants, that overexpress the transgene shows a phenotype that includes increased boll number, size and lint percentage in compare to the wild type plants; whereas in the second method transformed plants that reduced the transgene level produced plants with decreased number of cotton boll, size and lint percentage in compare to wild type and overexpression line both. q-RT PCR analysis showed that transgene transcript level was higher at fiber initiation stage (0 DPA) after that its level decreases throughout all developmental stages.
    Type: Application
    Filed: October 13, 2014
    Publication date: November 24, 2016
    Applicant: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
    Inventors: Samir Viswanath SAWANT, Rajiv Kumar TRIPATHI, Asif IDRIS
  • COMPOSITIONS AND METHODS FOR IMPROVING ABIOTIC STRESS TOLERANCE
    Publication number: 20160340688
    Abstract: The present invention relates to compositions and methods for improving the abiotic stress tolerance of plants. Plants and plant parts identified, selected and/or produced using compositions and methods of the present invention are also provided.
    Type: Application
    Filed: July 29, 2014
    Publication date: November 24, 2016
    Inventors: Zichao Li, Haiyan Xiong, Pengli Liu, Jingie Li, Hongliang Zhang
  • GLYPHOSATE-TOLERANT GENE AND USE THEREOF
    Publication number: 20160340689
    Abstract: Disclosed is a glyphosate-tolerant gene. The nucleotide sequence of the gene is SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5, and a mutant form of the gene that maintains the glyphosate-tolerant activity. The amino acid sequence of a protein encoded by the gene is respectively SEQ ID NO: 2, SEQ ID NO: 4, or SEQ ID NO: 6, and a mutant form of amino acids in a conserved region 1 of positions 280 to 294 and in a conserved region 2 of positions 416 to 433. Also disclosed is the use of the gene and the mutant form thereof in the production of a glyphosate-resistant/tolerant plant.
    Type: Application
    Filed: September 5, 2013
    Publication date: November 24, 2016
    Applicant: NANJING AGRICULTURAL UNIVERSITY
    Inventors: Sheng QIANG, Chanjuan MAO, Shiguo CHEN, Weimin DAI, Xiaoling SONG
  • NOV MINI-PROMOTERS
    Publication number: 20160340690
    Abstract: Isolated polynucleotides comprising a NOV mini-promoters are provided. The mini-promoter may be operably linked to an expressible sequence, including, but not limited to, reporter genes, genes encoding a polypeptide of interest, and regulatory RNA sequences such as miRNA, siRNA, and anti-sense RNA. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, including, but not limited to, for identifying or labeling cells, monitoring or tracking the expression of cells and gene therapy.
    Type: Application
    Filed: May 20, 2016
    Publication date: November 24, 2016
    Inventors: Elizabeth M. Simpson, Charles de Leeuw, Daniel Goldowitz, Wyeth W. Wasserman
  • ENHANCED NUCLEIC ACID CONSTRUCTS FOR EUKARYOTIC GENE EXPRESSION
    Publication number: 20160340691
    Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.
    Type: Application
    Filed: August 2, 2016
    Publication date: November 24, 2016
    Inventors: Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Kate Caves
  • TARGETED GENE DELIVERY FOR DENDRITIC CELL VACCINATION
    Publication number: 20160340692
    Abstract: Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.
    Type: Application
    Filed: February 24, 2016
    Publication date: November 24, 2016
    Inventors: Pin Wang, Lili Yang, David Baltimore
  • Muscle-Specific Nucleic Acid Regulatory Elements and Methods and Use Thereof
    Publication number: 20160340693
    Abstract: The present invention relates to nucleic acid regulatory elements that are able to enhance muscle-specific expression of genes, in particular expression in cardiac muscle and/or skeletal muscle, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy, more particularly muscle-directed gene therapy, and for vaccination purposes.
    Type: Application
    Filed: January 21, 2015
    Publication date: November 24, 2016
    Inventors: Thierry Vandendriessche, Marinee Chuah, Pieter De Bleser
  • METHODS AND COMPOSITIONS FOR PRODUCING OLEFINS
    Publication number: 20160340694
    Abstract: Compositions and methods for producing olefins are described herein. The olefins can be used to produced biofuels.
    Type: Application
    Filed: October 31, 2013
    Publication date: November 24, 2016
    Inventors: Murtaza F. Alibhai, Mathew A. Rude, Andreas W. Schirmer
  • METHOD OF PRODUCING ISOPRENE MONOMER
    Publication number: 20160340695
    Abstract: Isoprene synthase-expressing microorganisms that exhibit improved expression of pyrophosphate phosphatase are useful for producing isoprene monomer.
    Type: Application
    Filed: May 26, 2016
    Publication date: November 24, 2016
    Applicant: Ajinomoto Co., Inc.
    Inventors: Yoshinori TAJIMA, Akiko ONUKI, Hiroaki RACHI
  • VERSATILE EXTREMELY THERMOPHILIC BACTERIA FOR THE CONVERSION OF BIOMASS
    Publication number: 20160340696
    Abstract: Methods of producing ethanol by incubating lignocellulosic hydrolysates in the presence of cells of an isolated strain of Thermoanaerobactor at a temperature above 70 degrees Celsius. The strain of Thermoanaerobactor has a 16S rDNA sequence at least 99% identical a nucleic acid sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, and SEQ ID NO. 8.
    Type: Application
    Filed: August 9, 2016
    Publication date: November 24, 2016
    Inventors: Vitaly Svetlichnyi, Simon Curvers
  • Genetically Modified Yeast Species, and Fermentation Processes Using Genetically Modified Yeast
    Publication number: 20160340697
    Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.
    Type: Application
    Filed: July 5, 2016
    Publication date: November 24, 2016
    Inventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttila, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
  • GENETICALLY ENGINEERED YEAST CELL HAVING INCREASED NADPH PRODUCTION, METHOD OF INCREASING NADPH LEVEL IN YEAST CELL, METHOD OF PREPARING YEAST CELL, AND METHOD OF PRODUCING LACTATE USING YEAST CELL
    Publication number: 20160340698
    Abstract: Provided is a genetically engineered yeast cell having increased NADPH production, a method of increasing a NADPH level in a yeast cell, a method of preparing the genetically engineered yeast cell, and a method of producing lactate using the genetically engineered yeast cell.
    Type: Application
    Filed: March 10, 2016
    Publication date: November 24, 2016
    Inventors: Yukyung Jung, Kwangmyung Cho, Jinha Kim, Soonchun Chung
  • TYPE II FATTY ACID SYNTHESIS ENZYMES IN REVERSE ß-OXIDATION
    Publication number: 20160340699
    Abstract: This disclosure describes enzymes from the type II (a discrete set of enzymes) fatty acid synthesis (“FAS”) pathway that can be used in combination with thiolases to operate a functional reversal of the ?-oxidation cycle. A combination of thiolases with one or more of 3-oxoacyl-[acyl-carrier-protein] reductase (FabG, others), 3-hydroxyacyl-[acp] dehydratase (FabA, FabZ, others), and enoyl-[acyl-carrier-protein] reductase (FabI, FabK, FabL, FabV, others) yields a functional reversal of the ?-oxidation cycle. If only one or two enzymes are used, the remaining enzymes will be traditional beta oxidation enzymes. Once this cycle is coupled with the appropriate priming and termination pathways, the production of carboxylic acids, alcohols, hydrocarbons, amines and their ?-, ?-, and ?-functionalized derivatives from renewable carbon sources can be achieved.
    Type: Application
    Filed: January 26, 2015
    Publication date: November 24, 2016
    Inventors: Ramon Gonzalez, James M. CLOMBURG, Jacob E. VICK
  • MICROORGANISMS AND METHODS FOR THE PRODUCTION OF FATTY ACIDS AND FATTY ACID DERIVED PRODUCTS
    Publication number: 20160340700
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism
    Type: Application
    Filed: March 29, 2016
    Publication date: November 24, 2016
    Inventors: Hans Liao, Eileen Spindler, Joseph R. Warner, Michael Louie, Wendy Ribble, Brittany Prather, Ron Evans, Tanya E.W. Lipscomb, Michael D. Lynch
  • METHODS OF USING CAPSAICIN SYNTHASE FOR THE MICROBIAL PRODUCTION OF CAPSAICINOIDS
    Publication number: 20160340701
    Abstract: A biosynthetic method of making a capsaicinoid including expressing a first gene product of CS/AT3/Pun1 in a cellular system, growing the cellular system in a medium, and collecting the capsaicinoid. The biosynthetic method further includes expressing a second gene product of ACS1 and expressing a third gene product of pAMT in the cellular system.
    Type: Application
    Filed: January 16, 2015
    Publication date: November 24, 2016
    Applicant: Conagen Inc.
    Inventors: Hui Chen, Hongxue Wang, Oliver Yu
  • HEAT-STABLE, FE-DEPENDENT ALCOHOL DEHYDROGENASE FOR ALDEHYDE DETOXIFICATION
    Publication number: 20160340702
    Abstract: The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from Thermoanaerobacter pseudethanolicus 39E and displayed activity against a number of aldehydes including inhibitory compounds that are produced during the dilute-acid pretreatment process of lignocellulosic biomass before fermentation to biofuels. Methods to use the microorganisms and polypeptides of the invention for improved conversion of bio mass to biofuel are provided as well as use of the enzyme in metabolic engineering strategies for producing longer-chain alcohols from sugars using thermophilic, fermentative microorganisms.
    Type: Application
    Filed: March 3, 2016
    Publication date: November 24, 2016
    Applicant: UT-BATTELLE, LLC
    Inventors: James G. Elkins, Sonya Clarkson
  • ASTRAGALUS MEMBRANACEUS FERMENTATION PRODUCT AND METHOD OF PRODUCING THE SAME
    Publication number: 20160340703
    Abstract: Provided are an astragalus membranaceus fermentation product and a method of producing the astragalus membranaceus fermentation product, in which a lactic acid bacteria starter or a yeast starter proliferated and cultured in a medium is inoculated into a mixture, in which astragalus membranaceus, sugar, and water are mixed, to be fermented, and then a pH of a fermentation material obtained by fermenting is measured, and the fermentation material having a pH within a predetermined range is filtered and dried to obtain the astragalus membranaceus fermentation product.
    Type: Application
    Filed: November 24, 2015
    Publication date: November 24, 2016
    Applicants: CosisBio Co., Ltd.
    Inventors: Terry Cho HYUN, Sang-hurn PARK, Dong-goo KIM, Ji-young SHIN
  • METHOD OF MAKING A BENZYLISOQUINOLINE ALKALOID (BIA) METABOLITE, ENZYMES THEREFORE
    Publication number: 20160340704
    Abstract: There is provided a method of preparing a benzylisoquinoline alkaloid (BIA) metabolite comprising: a. culturing a host cell under conditions suitable for protein production, including a pH of between about 7 and about 10 said host cell comprising: b. a first heterologous coding sequence encoding a first enzyme involved in a metabolite pathway that converts (R,S)-norlaudanosoline into the metabolite; c. a second heterologous coding sequence encoding a second enzyme involved in a metabolite pathway that converts (R,S)-norlaudanosoline into the metabolite; d.
    Type: Application
    Filed: January 13, 2015
    Publication date: November 24, 2016
    Inventors: VINCENT MARTIN, LAUREN NARCROSS, ANDREW EKINS, ELENA FOSSATI, YUN ZHU