Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

Abstract: A chemiresistive biosensor for detecting an analyte can include a high specific surface area substrate conformally coated with a conductive polymer, and a binding reagent immobilized on the conductive polymer, wherein the binding reagent has a specific affinity for the analyte. The conductive polymer can be deposited on a substrate by oCVD.

Abstract: A large volume preconcentrator device for concentrating analytes. A housing accepts an analyte vapor flow, and a plurality of collection surfaces are disposed within the housing. A selectively actuatable heater is disposed on each of the plurality of collection surfaces. At least one selectively actuatable damper is disposed within the housing for selectively restricting a collection flow.

Abstract: An improved rapid diagnostic device, assay and multifunctional buffer reagent are provided for the detection of a target analyte in a fluid test sample. The 2-step assay utilizes a dual component flow-through device comprising a test unit and a dried indicator reagent delivery unit capable of receiving the fluid sample and multifunctional buffer, respectively. The test unit comprises a reaction zone containing immobilized capture reagent that can specifically bind to the target analyte, an absorbent zone supporting the reaction zone, and optionally, a blood separation zone in lateral fluid communication with the reaction zone. The dried indicator reagent delivery unit comprises a label zone permeated with a dried indicator reagent which is capable of being placed in transient fluid communication with the reaction zone of the test unit during the assay procedure.

Abstract: A device for incubating an immunoblot strip (6) includes at least one incubation channel (1) into which an elongate immunoblot strip (6) having at least one biological material on its front is inserted with its back facing a channel base (2). An agitating means (7), with which the immunoblot strip (6) is agitatable around a transverse axis (8) of the strip (6) at least at times during incubation via an agitating device (7). For handling and/or grouping incubation channels (1) with immunoblot strips (6) inserted, a rack (3) for taking up at least two incubation channels (1) is provided. The rack (3) is in operative connection with said agitating means (7) via load transmission points.

Abstract: Macroporous matrices containing molecularly imprinted photonic polymers (MIPPs) and methods of making these macroporous matrices are disclosed herein. The macroporous matrices can, for example, be used for detection of small molecules, such as metal ions, in a sample.

Abstract: A dew condensation sensor is described, including a nano-composite for generating local surface plasmon resonance, a light reflecting member disposed on one side of the nano-composite, a protection layer laminated on the light reflecting member, a light source/light receiver disposed facing the nano-composite, a spectroscope (or photo-detector) for detecting the light reflected by the light source/light receiver, a controller connected to the light source/light receiver and the spectroscope (or photo-detector) and used for overall control thereof, and a display unit connected to the controller. The dew condensation sensor detects occurrence of dew condensation based on the variation in the absorption spectrum, the absorption intensity or the reflected-light intensity of the local surface plasmon resonance of the nano-composite.

Abstract: Use of a programmable device with capacitive touch screen for the analysis of a fluid sample as interface between a micro-fluidic device, this micro-fluidic device comprising at least one electrode and at least one micro-channel comprising an input for the introduction of the sample, and the processor of said programmable device with a capacitive touch screen.

Abstract: The present application provides methods of producing soluble Treponema pallidum protein Tp0453 (such as the fragment shown in SEQ ID NO: 3). For example, the protein can be expressed from the pET28a vector in a cell and the resulting soluble Tp0453 protein isolated from the cell. Also provided are the isolated soluble Tp0453 protein, as well as Tp0453-Tp0326 chimeric proteins (e.g., SEQ ID NO: 11), and methods of using the proteins to detect antibodies specific for T. pallidum subsp. pallidum, for example to diagnose syphilis. Devices and kits that incorporate Tp0453 protein and Tp0453-Tp0326 chimeric proteins, such as lateral flow devices, are also disclosed. Kits that include the soluble Tp0453 protein and kits that include solid substrates containing the soluble Tp0453 protein are also provided.

Abstract: Provided herein are biological research methods, kits, and products that utilize radio frequency identifier technology.

Abstract: Disclosed herein is a thin-film layered centrifuge device and an analysis method using the same. One example of an embodiment of the present invention is a thin film layered centrifuge device where a device, such as a lab on a chip, a protein chip and a DNA chip, for diagnosing and detecting a small amount of material in a fluid is integrated into a rotatable thin-film layered body, and to an analysis method using the thin-film layered centrifuge device.

Abstract: The present invention relates to systems and methods for the rapid in situ determination of the existence of a hook effect and expansion of the dynamic range of a point of care immunoassay. For example, a system for identifying a hook effect and expanding the dynamic range of an immunoassay is provided that may include a primary sensor having first immobilized antibodies that may be configured to generate a first signal based on a presence or absence of a target analyte in a sample. The system may further include an attenuated sensor having second immobilized antibodies at a reduced concentration relative to a concentration of the first immobilized antibodies on the primary sensor and that may be configured to generate a second signal based on the presence or absence of the target analyte in the sample.

Abstract: The present invention relates to systems and methods for the rapid in situ determination of the existence of a hook effect and expansion of the dynamic range of a point of care immunoassay. For example, a system for identifying a hook effect and expanding the dynamic range of an immunoassay is provided that may include a primary sensor having first immobilized antibodies that may be configured to generate a first signal based on a presence or absence of a target analyte in a sample. The system may further include an attenuated sensor having second immobilized antibodies at a reduced concentration relative to a concentration of the first immobilized antibodies on the primary sensor and that may be configured to generate a second signal based on the presence or absence of the target analyte in the sample.

Abstract: A method of competitive immunoassay is provided.

Abstract: The invention provides methods and devices for generating optical pulses in one or more waveguides using a spatially scanning light source. A detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided. The system includes a scanning light source, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, a detector that is coupled to and in optical communication with the substrate, and means for spatially translating a light beam emitted from said scanning light source such that the light beam is coupled to and in optical communication with the waveguides of the substrate at some point along its scanning path. The use of a scanning light source allows the coupling of light into the waveguides of the substrate in a simple and cost-effective manner.

Abstract: The invention provides a device for detecting drugs of abuse or other compounds in saliva. The invention thus provides a device for detecting the presence of one or more analytes in a saliva sample, comprising: (a) One or more pre-treatment regions for specifically or non-specifically removing at least a part of the fraction of the saliva sample interfering with detection of the one or more analytes; and (b) A detection region comprising a biosensor surface, the surface comprising: molecules capable of specifically binding the one or more analytes; or the one or more analytes and/or analyte analogues.

Abstract: Microfluidic devices and methods including porous polymer monoliths are described. Polymerization techniques may be used to generate porous polymer monoliths having pores defined by a liquid component of a fluid mixture. The fluid mixture may contain iniferters and the resulting porous polymer monolith may include surfaces terminated with iniferter species. Capture molecules may then be grafted to the monolith pores.

Abstract: [Problem] An object of the present invention is to provide a method by which the amount of a very small amount of analyte (in particular, one having a sugar chain) can be measured with a high accuracy and a device which is used for said measurement method. [Means for Solution] The method for measuring the amount of an analyte according to the present invention is characterized in that a lectin labeled with a fluorescent dye, preferably said lectin whose dissociation rate constant [kd] is from 1.0×10?6 to 1.0×10?3 (S?1), is used as a secondary antibody in a sandwich assay using a surface plasmon excitation enhanced fluorescence spectroscopy [SPFS; Surface Plasmon-field enhanced Fluorescence Spectroscopy].

Abstract: [Problem] To provide a target substance detection chip, a target substance detection device, and a target substance detection method, that can be manufactured easily in a small size at low costs with reduction of the number of parts involved in the detection chip constituted by an optical prism and a detection plate used for a SPR sensor and an optical waveguide mode sensor, that can detect a target substance quickly with high sensitivity, and in which an analyte liquid is easily delivered.

Abstract: A sample plate comprising a sample well is disclosed. The sample well can comprise one or more bead retaining chambers. Also provided herein is a method of using the sample plate and kits comprising the sample plate.

Abstract: A microscope apparatus for detecting or imaging a target protein using a probe for intrinsic fluorescence resonance energy transfer (iFRET) according to the present invention comprises: a light irradiation unit that irradiates a first light having a wavelength range for exciting an amino acid in the target protein and a second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET; an objective lens that allows the lights irradiated from the light irradiation unit to be incident onto a sample into which the probe for iFRET is introduced; and a recognition unit that detects a first light emitting signal generated from the probe for iFRET by irradiating the first light having a wavelength range for exciting an amino acid in the target protein onto the sample and a second light emitting signal generated from the probe for iFRET by irradiating the second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET onto the sample, wherein the probe

Abstract: This invention discloses an immunochromatographic assay device to detect and quantify analytes. The device utilizes up-converting luminescent probes to detect time-resolved luminescence signals. Because the unconverting luminescent probes can have relatively long emission lifetime, background interference from sample autofluorescence and light scattering from excitation source can be easily eliminated through delayed luminescence detection. Furthermore, the up-converting luminescent probes can be excited by near Infrared (IR) or IR light sources. In comparing with UV and visible lights, the near IR and IR lights can penetrate deeper into sample matrices and more effectively excite the probes, but not the sample matrices, resulting in less background and higher detection sensitivity. A simple and low cost reader can be designed to measure the delayed up-converting luminescence of long lifetime that does not use expensive optical filters and mirrors.

Abstract: A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions.

Abstract: A device for identifying the presence of a specific target molecule or biomarker by the detection of a change in an electrical property includes a measurement sensor 8 comprising a semiconducting sensor structure 12 capable of conjugating with the biomarker, thus giving rise to the said change in electrical property, and an electrode system 3, 4 for conducting a signal from the device. According to the invention there is a further such sensor 9, of substantially identical form but having its sensor structure 14 already conjugated with the biomarker, or otherwise capped, e.g. using a further oligonucleotide strand, so as to act as an internal reference. When a biological sample, e.g. saliva, is applied to the electrodes, the reference enables the discounting of all environmental effects other than the biomarker. The invention provides a simple, cheap and accurate text for one or more biomarkers that can be used in the field without complex equipment.

Abstract: Provided herein are devices and methods for generating microfluidic gradients, including an array of unique microfluidic gradients within an array of microchannels. Fluids within conduits are mixed in an intersection region to generate a mixed flow stream in a source reservoir channel that provides a gradient that varies with axial distance from the intersection region. Microchannels having an inlet connected to the source reservoir channel are configured to provide a microfluidic gradient in the microchannel. An outlet end of the microchannel is connected to a sink reservoir channel. By varying the ratio of fluid flow rates from the fluid conduits, the microchannel gradients are tuned. In this manner, a large number of unique gradients or array of microfluidic gradients is provided, wherein the gradient can be any number of physical or chemical parameters, including concentrations and physical fluid properties.

Abstract: A method is described for obtaining antibodies binding specifically to an epitope resulting from in vivo modification of a biomolecule or biomolecular complex, where the in vivo modification results from expression of a transgene in an animal. An example of such a biomolecule or biomolecular complex is LDL and an example of such a transgene is human myeloperoxidase. Resulting antibodies and methods of using the antibodies are also described.

Abstract: This disclosure provides, among other things, a nanosensor comprising a substrate and one or a plurality of pillars extending from a surface of the substrate, where the pillars comprise a metallic dot structure, a metal disc, and a metallic back plane. The nanosensor comprises a molecular adhesion layer that covers at least a part of the metallic dot structure, the metal disc, and/or the metallic back plane and a capture agent bound to the molecular adhesion layer. The nanosensor amplifies a light signal from an analyte, when the analyte is specifically bound to the capture agent.

Abstract: The present disclosure, among other things, describes a reader system for analyzing one or more analytes in a fluid sample, comprising a casing (204) with at least one port (201) leading to a holster (301) which is configured to receive a cartridge (107) comprising a vertically oriented immunoassay device for analyzing one or more analytes in a fluid sample, an optical system (121) with excitation optics comprising a light source (302) and an excitation lens (305) configured to transmit light from the light source (302) and thereby excite a region of the vertically oriented immunoassay device when a cartridge (107) is placed in the holster (301), and collection optics comprising a photosensor (306) and a collection lens (307) configured to collect emitted light from the vertically oriented immunoassay device when a cartridge (107) is placed in the holster (301), an electromechanical motor system (106) configured to move the holster (301) in a vertical direction with respect to the optical system (121) so that

Abstract: The present disclosure relates to paper-based substrates and apparatus comprising such substrates. The apparatus may include a patterned conductive structure coupled to the paper-based substrate, wherein the patterned conductive structure responds to electromagnetic radiation.

Abstract: The present invention is directed to an aptamer comprising or consisting of the nucleic acid sequence of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and/or a nucleic acid sequence being at least 80% identical to one of SEQ ID No. 1, 2 and 3 for use in therapy and/or diagnosis of autoimmune diseases, wherein the autoimmune disease is cardiomyopathy, dilated cardiomyopathy (DCM), peripartum cardiomyopathy (PPCM), idiopathic cardiomyopathy, Chagas' cardiomyopathy, Chagas' megacolon, Chagas' megaesophagus, Chagas' neuropathy, benign prostatic hyperplasia, scleroderma, psoriasis, Raynaud syndrome, pre-eclamsia, kidney allograft rejection, myocarditis, glaucoma, hypertension, pulmonary hypertension, malignant hypertension, and/or Alzheimer's disease.

Abstract: Apparatus and method for detecting a presence of a subject material in a fluid sample using at least one resonating sensor immersible in the fluid sample. Binding kinetics of an interaction of an analyte material present in the fluid sample are measured with the resonating sensor, which has binding sites for the analyte material. Prior to exposing the resonating sensor to the fluid sample, operation of the resonating sensor is initiated, which produces a sensor output signal representing a resonance characteristic of the resonating sensor. Optionally, a reference resonator is used that produces a reference output signal. The reference resonator lacks binding sites for the analyte. Introduction of a fluid sample to the resonating sensor is automatically detected based on detection of a characteristic change in the sensor output signal or a reference output signal, or both.

Abstract: Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Abstract: Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

Abstract: A method of fabricating polymer single nanowires, comprising the steps of: spin coating a polymethylmethacrylate resist onto a silicon wafer patterned with at least one gold electrode pair; creating a nanochannel using e-beam lithography between each pair of the at least one gold electrode pairs; placing the silicon wafer into an aniline monomer polymerization solution; reacting the polymerization solution to give a coated wafer and a polyaniline film; and cleaning the coated wafer of polymethylmethacrylate resist and polyaniline film to give at least one gold electrode pair with a connecting polymer single nanowire.

Abstract: A method for forming a filter in a fluid flow path in a microfluidic device is provided. The method includes introducing a photopolymerization reaction solution into the microfluidic device; and performing polymerization of photopolymerization reaction solution to form a filter in the fluid flow path in a microfluidic device.

Abstract: Various embodiments provide a system for detecting one or more analytes in a fluid. The system comprises: a controller adapted to obtain one or more sample streams of the fluid. The controller is configured in use to form a plurality of output streams. Each output stream comprises at least part of one of the one or more sample streams. The system further comprises: a detector adapted to receive from the controller the plurality of output streams and comprising a plurality of sensors. Each sensor is operable to detect an interaction between a corresponding detecting agent and a corresponding analyte. The detector is configured in use to detect one or more said interactions using the plurality of output streams to determine if the fluid contains one or more said analytes. A corresponding method is also provided.

Abstract: This invention describes a method and apparatus for actuation and multiplexed sensing using an array of sensing elements. The invention can be used for label-free detection of biological and chemical agents in a robust, miniaturized package. The invention integrates photonics, CMOS electronics, and Micro/Nano system technologies and allows multi-analyte sensing in the same package. The preferred actuation method is using magnetic thin films and preferred sensing method is optical using interference means.

Abstract: An impedance biosensor for sensing concentration of a target analyte in a solution includes an insulator substrate, electrically coupled conductive trace units on the substrate, biological sensing films, and an insulator cover. Each trace unit has a first trace and a second trace, each having a sensing end portion and a connecting end portion. The biological sensing films are disposed on the sensing end portions, and have a capture layer for capturing the target analyte. The insulator cover covers the trace units and is formed with window openings that expose the sensing end portions and that cooperate with the insulator substrate to define a space for receiving the solution therein.

Abstract: The present invention relates to a non covalent molecular structure comprising a carbon nanostructure and a pyrene based glycoconjugate (I) which is linked to the said carbon nanostructure by a non covalent link, the said glycoconjugate (I) having the formula: wherein B is a group which is present on any of the ten carbon atoms of the pyrene structure represented in (I) susceptible to bear a substituent, and is represented by the following group: —(CH2)n-CO—NH-A, wherein n is an integer from 1 to 9, A is a group of formula: The present invention also relates to an electronic device comprising the said non covalent molecular structure, and to the use of this device for the detection of a lectin involved in bacterial or viral infections. Thus the invention also relates to a method for detecting the presence of a lectin in a sample to be analysed.

Abstract: Disclosed is a fluidic assay device for assaying at least one property of a liquid sample, the device comprising: (i) a liquid sample application region; (ii) at least one test flow path in liquid flow communication with the sample application region; (iii) a reference flow path in liquid flow communication with the sample application region; and (iv) a junction region, at which the test flow path and the reference flow path contact one another, the junction region typically comprising an outlet, conduit, chamber or other portion which permits the onward flow of liquid; wherein a liquid flowing along the reference flow path, upon reaching the junction region, has the effect of preventing the flow of liquid along the test flow path. The invention relates to a fluidic device for the passage of a liquid. It also relates to an assay device suitable for measurement of the amount and/or presence of an analyte in, or property of, a fluid sample.

Abstract: Physical changes resulting from an association between a template molecule and a target molecule are detected by monitoring changes in the template molecule. Exemplary changes include a change in a physical dimension or stiffness of the template molecule, a change in electrical conductivity of the template molecule and a change in the energy required to dissociate the target molecule and the template molecule. The magnitude of the change is indicative of the specific identity of the target molecule.

Abstract: The invention relates to compositions and methods for the immunoassay of an analyte of interest. The analyte is detected in an immunoassay using three or more antibodies, wherein each antibody specifically binds to a different epitope on the analyte. When the analyte of interest in a clinical marker for an acute disease, the detection of the analyte by immunoassay is a diagnosis of the occurrence of the disease.

Abstract: A system and method are provided for utilizing radiometric fluorescence detection to determine a glucose independent concentration value when measuring frequency bands that do not contain the system isosbestic point. Preferably two bands are chosen such that a first band is below the system isosbestic point, and a second band is above the system isosbestic point, and both points are sufficiently far from the frequency endpoints to maximize the signal to noise ratio.

Abstract: A method for providing quality control on a lateral flow assay device or for triggering a process-related step, the device including a substrate having at least one sample receiving area, at least one reagent zone downstream and in fluid communication with the at least one sample receiving area, at least one detection zone downstream and in fluid communication with the at least one reagent zone and at least one wicking zone downstream of the at least one detection zone, each fluidly interconnected therewith along at least one fluid flow path. The detection material provided in the at least one reagent zone produces a detectable signal that can be tracked and monitored prior to the completion of at least one test being performed on the lateral flow assay device.

Abstract: A biosensor for use in detecting the presence of diseases, the biosensor comprising a detector for detecting a presence of at least one marker indicative of a specific disease. A method of determining efficacy of a pharmaceutical for treating a disease or staging disease by administering a pharmaceutical to a sample containing markers for a disease, detecting the amount of at least one marker of the disease in the sample, and analyzing the amount of the marker in the sample, whereby the amount of marker correlates to pharmaceutical efficacy or disease stage. Markers for gynecological disease. An immune-imaging agent comprising labeled antibodies, whereby the labeled antibodies are isolated and reactive to proteins overexpressed in vivo. Informatics software for analyzing the arrays, the software including analyzing means for analyzing the arrays.

Abstract: Techniques for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte are disclosed. A microdevice useful for the disclosed techniques includes a semi-permeable membrane structure, a substrate, a first and second microchambers formed between the membrane structure and the substrate. The first microchamber can be adapted to receive a solution including the polymer, and the second microchamber can be adapted to receive a reference solution. Environmental target analyte can permeate the semi-permeable membrane structure and enters the first microchamber and the second microchamber. Based on the difference in a property associated with the polymer solution that is responsive to the target analyte-polymer binding, and the corresponding property associated with reference solution, the presence and/or concentration of the target analyte can be determined.

Abstract: A sensor device comprises a dielectric substrate (52); and a metal layer (53) on the substrate (52) with at least one array of cavities (54) therein and adapted to support L-SPR, each of the cavities (54) in the metal layer (53) having an opening (56) and a closed bottom (58) and widening from opening to bottom. A bed of dielectric material (62) is provided over the bottom (58) of each cavity (54) to reduce its apparent depth, the bed surface (62) being functionalized to bind to receptor moieties (64). This sensor device is particularly designed for SPR detection, but can be used in other detection techniques.

Abstract: This invention describes a design of a lateral flow assay device that detects dried chemicals or trice volume aqueous sample solutions, applicable for detecting body fluids and dried or liquid chemicals. The dried or aqueous samples on the sample loading area will contact with a secondary aqueous solution in described manner and flow to the reaction area. This invention enables a complete lateral flow assay while the sample volume itself is too small to accomplish a complete lateral flow test.

Abstract: A microstructured measurement chip (1) for optical measurement of properties of artificial or biological membranes (40), having a lower, translucent support layer (10) and at least one non-translucent main layer (20) disposed on top of the former, which layer has depressions (30) configured as measurement chambers, having an upper opening (25) and one or multiple inner side walls (26). In order to improve the measurement chip (1) in such a manner that biological systems can be measured with greater measurement accuracy and higher throughput, it is proposed that the side wall or the side walls (26) of the measurement chambers (30) have depressions (27) and/or elevations (28). The invention furthermore relates to a holder (200) for the measurement chips (1) as well as to a method for the production of the measurement chips (1) from a silicon wafer (300).

Abstract: The invention relates to a substance determining apparatus (19) for determining a substance within a fluid. A location event determination unit (34) determines location events indicating that a particle, which may have attached the substance, is located in a sensing region and a property of the location events from the sensing signal generated by sensing the sensing region. A location events selection unit (70) selects a part of the location events having a property in a predefined property range, and a substance determination unit (40) determines the substance within the fluid (3) based on the selected part of the location events. This allows tuning the determination of the substance within the fluid to a desired property range, which can relate to a desired kind of bound particles, thereby reducing the influence of unwanted effects on the determination of the substance and, thus, improving the determination accuracy.