Abstract: An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.

Abstract: A method of quantifying the amount of glucose in a sample is provided herein that may further comprise an interferent such as mannitol. At least two measurements are obtained using measurement methods that differ in their sensitivity to the amount of interferent in the sample, thus enabling the results to be compared to determine whether any interferent is present in the sample.

Abstract: A system for measuring an evanescent wave phenomenon at total internal reflection, the system comprising: a) a sensing surface comprising a plurality of areas of interest; b) an illumination sub-system comprising a light source, which illuminates each area of interest on the sensing surface over a range of angles of incidence; c) a detector which responds differently to an intensity of light received by it at different locations; and d) projection optics comprising primary optics and a plurality of secondary elements, the primary optics projecting an image of the illuminated sensing surface onto the secondary elements, which project their received light onto the detector in such a way that it is possible to determine, from the response of the detector, how much light is reflected from each area of interest, as a function of angle of incidence over the range of angles for that area.

Abstract: A biosensor and method for detecting a target material using an agglomeration of magnetic nanoparticles are provided.

Abstract: There is described a system for testing a sample. The system comprises a microplate, at least one actuator, a plurality of mutually spaced sensors, and a processor. The microplate has a test portion including an interactive substance. The interactive substance is inherently interactive with a specified test substance. The microplate is arranged such that at least the test portion of the microplate may be brought into contact with the sample. The at least one actuator is operable to vibrate the microplate. The plurality of mutually spaced sensors are coupled to the microplate. Each sensor is operable to provide a respective sensory data time series during vibration of the microplate. The microplate and the sensors are arranged such that the provided sensory data time series are not independent from one another.

Abstract: A radial flow immunoassay includes a membrane having a plurality of immobilized distinct antigens disposed thereon. Methods of producing the immunoassay, and methods of utilizing same to detect immunoglobulin present in a biological sample, are also disclosed.

Abstract: It has been found that certain alleles of the human MYH6 gene are predictive of risk of certain conditions, including Sick Sinus Syndrome, Atrial Fibrillation, Pacemaker implantation and Thoracic aortic aneurysm, in humans. The invention provides diagnostic applications using such alleles, including methods of determining a susceptibility of Sick Sinus Syndrome and related conditions.

Abstract: Expedient and sensitive detection of a detection target substance is enabled in target substance detection that detects the detection target substance at a sensor portion provided within a flow channel. A detection target substance detecting method for a detecting detection target substance which may be contained in a liquid sample emits ultrasonic waves that propagate in a direction transverse to a flow channel when the liquid sample flows within a ultrasonic wave emission region at which ultrasonic waves can be emitted, to concentrate the detection target substance at a wall surface of the flow channel toward the side of the sensor portion and to detect the detection target substance.

Abstract: Sandwich separation is based on forming a sandwich complex with a magnetic bead, buoyant bead, and a target. Once a sandwich formation is created, the sandwich can be separated using its dual physical properties, namely magnetism and buoyancy. Sandwich separation is highly specific, allows for removal of the beads that do not have any attached target, and reduces the number of background beads. Sandwich separation can also be used to allow for target detection in raw specimen. Also, improvement of detection capability is accomplished by performing AMBR measurements on a solid interface, where the rotational period speeds up and allows for dramatically reduced time-to-result.

Abstract: The present invention relates to measures for determining glucose and for diagnosing diseases based on impaired glucose metabolism. In particular the present invention relates to a device comprising a hydrogel having a glucose-binding protein and a ligand of the glucose-binding protein incorporated therein, wherein the hydrogel comprises a first hydrogel matrix made of alginate and a second hydrogel matrix which forms an interpenetrating network within the first hydrogel matrix. The invention further relates to the use of such a device for determining the glucose content in a sample and to the use of the device for diagnosing impaired glucose metabolism in a test subject.

Abstract: Devices, methods and kits for the detection of analytes in liquid samples. A device for conducting an assay to determine the presence or amount of an analyte in a fluid sample having a flow path matrix that facilitates fluidic flow and a fluid impermeable barrier. The flow path includes a first region on a top side of the barrier that facilitates fluid flow in a first direction and comprises a sample entry area and a second region on a bottom side of the barrier that facilitates fluid flow in a second direction substantially opposite the first direction and comprises a detection zone. Methods using the devices. Kits including the devices and various reagents necessary for conducting the methods.

Abstract: An SPR sensor comprising a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate, a light source that illuminates an interface between the conducting layer and the substrate, a photosensitive surface that generates signals from light reflected from the interface, a flow cell formed with at least one flow channel having a lumen defined by a wall formed from an elastic material and from a region of the conducting layer, and at least one hollow fluid-providing flow control apparatus having a lumen and an orifice communicating with its lumen. Fluid flow is enabled between the flow channel and the lumen of the flow control apparatus by forcing an end of the flow control apparatus through the elastic material so that the orifice communicates with the flow channel lumen.

Abstract: Embodiments of the present disclosure set forth a sensor for detecting a target of interest. One exemplary sensor may comprise a container; a probe disposed in the container; a circulation means configured to circulate substances in the container; a light source; a light receiver; and a detector configured to generate an electrical signal, the magnitude of which reflects the amount of light that is received by the light receiver, wherein the sensor is located between the light source and the light receiver, and wherein the sensor is configured such that the probe and the path of the light emitted by the light source are in different regions inside the container.

Abstract: A diagnostic device includes a photodiode (2) formed by a body (10) of semiconductor material having a first surface (6a), an integrated optical structure (30) on the first surface and having a second surface (34a), and at least one detection region (50) on the second surface. The at least one detection region includes at least one receptor (52) that binds to a corresponding target molecule (MB) that can be mated with a corresponding marker (54), which, when excited by radiation having a first wavelength (?e), emits radiation having a second wavelength (?f) that can be detected by the photodiode. The integrated optical structure includes at least a first layer (34, 62) of a first material having a first refractive index (n1). The first layer has a thickness substantially equal to an integer and odd multiple of one fourth of the first wavelength (?e) divided by the first refractive index.

Abstract: A lateral flow device for use in a mainframe or point-of-care clinical analyzer, in which the lateral flow device includes a planar support having at least one sample addition area and at least one reaction area disposed thereon. The sample addition area and reaction area are fluidly interconnected to one another and form at least one lateral fluid flow path. The lateral flow device is sized for retention within a storage cartridge of the analyzer defined by a hollow interior and having a plurality of lateral flow assay devices retained in stacked relation therein.

Abstract: A tip for use in an optical detection system to analyze an analyte in a fluid sample drawn into the tip, using light reflected from a detection surface inside the tip that the analyte binds to, comprising a first detection surface and a second detection surface located in a same flow path with no controllable valve separating them, wherein the first and second detection surfaces have different surface chemistries.

Abstract: Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses.

Abstract: A sensor comprises a light source that emits light, a light receiver for receiving light, a sampling unit for binding the target of interest disposed between the light source and the light receiver, a light selecting unit for allowing light of a predetermined wavelength be received by the light receiver, and a detector configured to generate an electrical signal, and the magnitude of which reflects the amount of light that is received by the light receiver. The sampling unit comprises a substrate, a material disposed on the substrate, and a probe disposed on the material and configured to bind to the target of interest. The sampling unit is configured such that the probe is in the path of the light emitted by the light source.

Abstract: Embodiments of the present disclosure set forth an apparatus of a sensor for detecting a target of interest. One example apparatus may comprise a substrate, a material disposed on the substrate and a probe disposed on the material. The probe is configured to bind to the target of interest and scatter light emitted from a light source when the target of interest is bound to the probe.

Abstract: The invention utilises the techniques and instruments which are typically used in flow cytometry (FC) as a tool for analysing adsorbed materials. Thus the invention provides a method for analysing a component which is adsorbed to an insoluble metal salt, comprising steps of: (i) labelling the adsorbed component with a binding reagent; and (ii) analysing the labelled adsorbed component by flow cytometry.

Abstract: Devices and methods for gravimetric sensing are disclosed. A gravimetric sensor includes a piezoelectric resonator and an encapsulating layer formed on the surface of the resonator. The encapsulating layer defines a channel within the encapsulating layer on the surface of the resonator. The sensor is fabricated by forming a piezoelectric resonator, forming a sacrificial layer on a surface of the piezoelectric resonator, forming an encapsulating layer over the sacrificial layer on the resonator, and etching the sacrificial layer to remove the sacrificial layer and form a channel on the surface of the resonator. The sensor is used by supplying the liquid to the channel of the gravimetric sensor, operating the piezoelectric resonator, detecting a change in a resonant frequency of the resonator, and determining a presence of the analyte in the liquid from the change in resonant frequency of the resonator.

Abstract: A multiple-substance-responsive substance is disclosed, which is capable of simultaneously detecting a plurality of detection target substances by a single measurement. By a multiple-substance-responsive gel including: a plurality of kinds of complexes including (i) specifically binding substances, and (ii) binding partners each specifically and reversibly binding to a corresponding one of the specifically binding substances; and a polymer gel to which the plurality of kinds of complexes are immobilized so as to form cross-links, the plurality of kinds of complexes each being formed by binding between (i) a specifically binding substance among the specifically binding substances and (ii) a corresponding binding partner among the binding partners, a plurality of detection target substances can be simultaneously detected by a single measurement.

Abstract: Embodiments of the present disclosure set forth a sensor for detecting a target of interest. One exemplary sensor may comprise a container; a probe disposed in the container and configured to bind to a target of interest; a circulation means configured to circulate substances in the container; a light source; a light receiver; a light selecting unit for allowing light of a predetermined wavelength be received by the light receiver; and a detector configured to generate an electrical signal. The magnitude of the electrical signal reflects the amount of light that is received by the light receiver. The container is located between the light source and the light receiver. Moreover, the container is configured such that the probe and the path of the light emitted by the light source are in different regions inside the container.

Abstract: The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analyses due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area.

Abstract: The present invention relates to the use of surfaces that exhibit different surface energies wherein the difference in surface energies is configured to disrupt capillary laminar flow of a fluid travelling between the two surfaces. The invention further relates to the use of such surfaces in assay methods including a device utilising same.

Abstract: The invention is a surface plasmon resonance (SPR) sensor to determine the presence and quantity of biological or chemical entities in an analyte. The sensor comprises a metal periodic structure deposited as a thin layer of a noble metal, comprising a one dimensional array of nanoslits or a two dimensional array of nanoholes on a transparent dielectric substrate, a nm-thick layer of transparent dielectric protection layer on top of the metal periodic structure and a functionalization layer, which acts as a binding layer to biological or biochemical entities in an analyte that is in contact with the functionaliztion layer.

Abstract: A method of manufacturing a biopolymer sensor including providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, adding a biological material in the biopolymer matrix, providing a substrate, casting the matrix solution on the substrate, and drying the biopolymer matrix solution to form a solidified biopolymer sensor on the substrate. A biopolymer sensor is also provided that includes a solidified biopolymer film with an embedded biological material.

Abstract: A Raman scattering probe, and a method of making such a probe, uses a capsule of nanometric size, such as a nanotube, to which is coupled at least one Raman-active molecule. The Raman-active molecule may be encapsulated in, or attached on the exterior of the capsule, and exhibits a Raman scattering response when the probe is illuminated by an excitation light beam. A functionalization chemical group that is attached to an exterior of the capsule provides a connection between the capsule and a target material. This functionalization may include a generic chemical functionalization that bonds with any of a plurality of secondary chemical groups each of which bonds directly with a different target. A method of using the probe for Raman spectroscopy or Raman imaging is also provided.

Abstract: The invention relates to an analysis system for analyzing a sample on a test element. The system has an analysis unit for generating a signal as a function of an analyte contained in a sample, and a detection unit for detecting the signal. The analysis system further includes a test element holder into which the test element can be reversibly introduced and in which it can be positioned relative to the analysis unit and the detection unit. The test element contains at least one guide element, which is suitable for laterally guiding the test element, so that the test element in the test element holder is held and guided only on an outer region of the test element, and an inner region of the test element introduced into the test element holder remains free. The test element contains a sample application site in the inner region.

Abstract: The invention makes it possible to measure binding of a biochemical substance with a high throughput and with high sensitivity using a small cell capable of being filled with a small amount of chemical solution. A space between a first substrate and a second substrate such that probes are immobilized on their mutually facing planes is used as a cell that houses a specimen solution. Light is irradiated from a first substrate side, and reflected light is subjected to spectroscopy. Binding of the target with the probe is detected by a wavelength shift in the refection spectrum.

Abstract: A biochip comprising a plastic substrate, an IC chip, and a sealing cover is disclosed in this invention. The plastic substrate combines the function of sample inlet area, separating structure, micro-fluidic channel, flow resistor, detection area, and capillary pump or suction area. Sealing the plastic substrate with porous cover could make the structure of micro-fluidic to form the capillary effect or degas-driven effect, and drive the sample naturally without extra pump. The detection area is constituted by the IC chip which is embedded into the plastic substrate, and the IC chip includes the amplifier circuit and detection structure. In the detection area, there uses the biological specific conjugates to catch the bio-particles, nano-particles, or macromolecule sensitively. And finally, transfer the detected electric signal to a mobile communication device.

Abstract: The invention describes a family of sensors for assaying macro-molecules and/or biological cells in solution. The invention also describes methods of making and using the sensors. Each sensor has the form of a well (a hollow cylinder having a floor but no lid) or a trench whose walls comprise a plurality of GMR or TMR devices. Suitably shaped magnets located below each well's floor pull labeled particles into the well/trench and up against the inner wall where a field gradient orients them for optimum detection. Any unattached labels that happen to also be in the well/trench are removed through suitably sized holes in the floor.

Abstract: A device for detecting an analyte in a sample, which includes a radiation source adapted to generate electromagnetic radiation; a transducer capable of transducing a change in energy to an electrical signal; at least one tethered reagent on or proximal to the transducer, the tethered reagent having a binding site which is capable of binding the analyte; a chamber for holding the sample in fluid contact with transducer, wherein the chamber is adapted to contain the sample on inversion, partial inversion or agitation of the device; and a detector which is capable of detecting the electrical signal generated by the transducer.

Abstract: A component or device is provided for the detection or the measurement in parallel of one or more specific types of biological or chemical target products. This component includes a group of nanotubes selected and/or functionalized to interact with the target product, around an optical waveguide. Thus, an optical coupling is produced between the optical waveguide and one or more optical characteristics of these nanotubes, the modifications of which are evaluated in the presence of the target product. In addition, a method is provided for manufacturing and preparing such a component or device, and a detection method using them, as well as a post-manufacture preparation method comprising a specific functionalization for different target products starting from the same type of pluripotent generic component. Also provided is a family of PFO-based functionalization polymers.

Abstract: Disclosed are methods, systems, and apparatuses for the free solution measurement of molecular interactions by backscattering interferometry. In one aspect, the invention relates to method and systems for detecting molecular interaction between analytes in free-solution wherein the analytes are label-free and detection is performed by back-scattering interferometry. Also disclosed are label-free, free-solution, and/or real-time measurements of characteristic properties and/or chemical events using the disclosed techniques. The disclosed methods can have very low detection limits and/or very low sample volume requirements. Also disclosed are various biosensor applications of the disclosed techniques. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: An optical biosensor including a bio-sensing unit configured to receive an optical signal and generating a sensed optical signal, the wavelength of which varies according to a result of sensing a biomaterial; and a spectrometer including a plurality of ring resonators for dividing the sensed optical signal according to a wavelength and generating a plurality of output optical signals, respectively.

Abstract: A complex including: a support provided with at least two faces one of which is provided with a coating of an adhesive, at least one ligand, said ligand being immobilized on the adhesive surface. The ligand implemented within the framework of the present invention is chosen from among proteins, peptides, antibodies, nucleic acids, sugars or oligosaccharides, toxins, pesticides, hormones, herbicides, fungicides, neurotransmitters.

Abstract: A rapid test apparatus, system, and method of use utilizing lateral flow immunoassay (LFIA) detection of a selected ligand in a liquid sample from a body fluid such as saliva in a pet in which antibodies and their complimentary antigens are used with detection-nanoparticles to provide a visual or measurable end point indicator in which the method measures the exposure to viruses in the canine from Canine Parvovirus (CPV) and/or Canine Distemper virus (CDV).

Abstract: According one embodiment, an optical waveguide measurement system includes a first optical waveguide immobilizing a first substance, the first substance being able to be specifically bound to glycated hemoglobin; a plurality of first magnetic microparticles immobilizing a second substance immobilized, the second substance being able to be specifically bound to the glycated hemoglobin at a first site different from a second site, and the first substance can be specifically bound to the glycated hemoglobin at the second site; a first magnetic field applying section provided above the first optical waveguide and being able to move at least one of the plurality of first magnetic microparticles by magnetic force; a first light source being able to inject light into the first optical waveguide; and a first light receiving element being able to receive light ejected from the first optical waveguide.

Abstract: A portable detection system for allergic diseases includes a filtration-based inspection module and a reader module. The filtration-based inspection module includes an FPW sensor and a liquid sample filtration apparatus, wherein the liquid sample filtration apparatus includes an injection opening, a passage module, a filtering membrane and a gathering aperture. The injection opening is in communication with the gathering aperture. The FPW sensor comprises a frame body, a carrier and a sensing chip having an accommodating slot in communication with the gathering aperture. The carrier comprises a plurality of conductive terminals, and the conductive terminals are electrically connected with the sensing chip. The reader module comprises a connection slot capable of being inserted by the conductive terminals of the carrier.

Abstract: The present invention relates to a microfluidic based assay system, comprising a disposable assay cartridge and associated reading device, as well as the individual components themselves. The present invention also relates to methods of conducting assays, using the cartridge and device of the invention, as well as kits for conducting assays.

Abstract: The present invention relates generally to an assay device with improved tolerance to mishandling and/or use by the user. More particularly, the invention relates to an assay device with reduced susceptibility to flooding.

Abstract: A flow through cartridge for solid phase extraction of an analyte from a liquid has a cartridge body bounding a passage for retaining a sorbent. The passage extends from a first end face to a second end face, opposite the first end face of the cartridge body. The cartridge body is composed of a core of a first material extending around the passage and sealing rings of a second material, extending around an axis of the passage at the first and second end faces. Surface portions of the sealing rings constituting surface portions of the first and second end faces. The first material is stronger and stiffer than the second material.

Abstract: Provided herein is a microfluidic device and related method for controlling flow of different fluid components of a fluid. The microfluidic device comprises an input channel, focusing channel and an assaying channel. The microfluidic device is adapted to separate a fluid into at least two fluid components, and is further adapted to detect a target material comprised within one of the fluid components. The method comprises providing a channel, the channel having a dimension which is a function of a dimension of one of the fluid components and deliver the fluid through the channel at a set flow rate.

Abstract: The present invention relates to functional, modified glucose-galactose binding proteins (GGBPs), that have a greater melting temperature (Tm) than a reference GGBP. The present invention also relates to biological sensors, e.g., glucose sensors, comprising these thermostable GGBPs. The present invention also relates to nucleic acids encoding these thermostable GGBPs.

Abstract: The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed.

Abstract: The present invention relates to an encoded microcarrier comprising a readable code attached to it for identification, said encoded microcarrier comprising a body having at least a detection surface to detect a chemical and/or biological reaction, the microcarrier further comprising at least a spacing element projecting from the body and shaped to ensure that, when the encoded microcarrier is laid on a flat plane with the detection surface facing said flat plane, a gap exists between said flat plane and the detection surface. The invention also relates to an assay device designed to use a plurality of said encoded microcarriers to perform assays. The invention relates finally to a method for monitoring a chemical or biological reaction.

Abstract: A chemical modification method of a defined area on a substrate surface, comprising the steps of: a) providing a flow cell having a reactive substrate surface, b) providing a laminar flow of a first fluid comprising a first reagent and a laminar flow of a second fluid comprising a second reagent, adjacent to the flow of the first fluid, such that the two laminar fluids flow together over the reactive substrate surface with an interface to each other and, c) dynamically controlling the relative flow rates of the first and second fluids to position the interface so that a predetermined area of the reactive substrate surface is repeatedly exposed to both the first and the second fluid to obtain a chemically modified predetermined area of the substrate surface.

Abstract: This invention relates to a cartridge for an immunoassay test. The cartridge comprises (a) a probe well comprising a probe and a cap, the cap being in a closed position to enclose the probe in the probe well, wherein the probe has a bottom tip coated with analyte-binding molecules; (b) a sample well to receive a sample; (c) one or more reagent wells; (d) a plurality of wash wells each containing a first aqueous solution; and (e) a measurement well having a light transmissive bottom, the measurement well containing a second aqueous solution; wherein the openings of the sample well, reagent well, measurement well and wash wells are sealed. The present invention also relates to an apparatus for loading and releasing a probe. The apparatus comprises a push pin and a groove to load and transfer the probe to a plurality of locations, such as different wells in the above-mentioned cartridge, to conduct the immunoassay test.

Abstract: A bio-molecule detecting device that enables high-sensitivity measurement is provided. The bio-molecule detecting device was configured so that the orientation directions of free molecules and binding molecules in a solution are switched by switching the radiation direction of orientation control light, whereby the light extinction amount can be changed using nanorods associated with the free molecules and the binding molecules. In addition, between the free molecules and the binding molecules, there is a difference in the necessary time for the orientation direction to be switched by switching the radiation direction of the orientation control light, and therefore the timings, at which the light extinction amounts by the respective molecules change, are different.