Abstract: Disclosed is an antibody which binds to olanzapine, which can be used to detect olanzapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of olanzapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.

Abstract: Disclosed is an antibody which binds to paliperidone, which can be used to detect paliperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of paliperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, risperidone and paliperidone in a single lateral flow assay device.

Abstract: A test device and method for colored particle immunoassay. A colorimetric monoclonal antibody is disposed on a receiving piece having a simple structure or in an entrance of a through-hole of a transparent tubular body, such that the monoclonal antibody bonded to micro gold very rapidly reacts with an antigen of a liquid sample. The liquid sample can arrive at a C site and a T site without either a filter or a permeable material having capillary tubes. The C site and the T site are disposed on the interior of the through-hole. This simplifies the structure, thereby reducing the cost of manufacture. The liquid sample can rapidly arrive at the C site and the T site through the through-hole of the transparent tubular body without, so that antibodies of the C site and the T site can react with the antigen in a very short time.

Abstract: Disclosed is an antibody which binds to quetiapine, which can be used to detect quetiapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of quetiapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.

Abstract: Provided are a biosensor and a biomaterial detection apparatus including the same. The biomaterial detection apparatus comprises a light source to provide quantized photons; a substrate spaced apart from the light source; a single photonic sensor layer disposed on the substrate to sense the photons; and an adsorption layer disposed to cover the single photonic sensor layer, allow the photons to pass therethrough, and adsorb a biomaterial between the light source and the substrate.

Abstract: The invention relates to a method and a sensor device (100) for evaluating an assay with a sample. During the assay, optical measurements are made at a sensing surface (112), and at least one “homogeneity-image” of the sensing surface (112) is generated. From this image, an “homogeneity-indicator” is determined for at least one region of interest, and the optical measurements are then evaluated in dependence on said indicator. The homogeneity-indicator may for example be a binary value which indicates if an inhomogeneity was detected or not. If an inhomogeneity was detected, all optical measurements may be rejected, only measurements for the involved region of interest may be rejected, or measurements for a selected sub-area of the involved region of interest(ROI) may be rejected.

Abstract: Incorporation of modified branched polymers in one or more components of assays enhances sensitivity, specificity or both, providing powerful point of use tests.

Abstract: Provided is a device with a nanopore that has a thiol-containing material bound to a gold layer, methods of producing the devices, and methods of analyzing nucleic acid using the devices.

Abstract: Detection of miniscule amounts of nucleic acid is accomplished via binding of target nucleic acid to probe material, composed of nucleic acid, which is bound to a sensor configured to sense mass. The sensor is prepared by immobilizing a probe material to a surface of the sensor, wherein the probe material is known to bind to the target nucleic acid. The prepared sensor is exposed to the target nucleic acid. The target nucleic acid binds to the probe material. The mass accumulated on the sensor reflects the amount of target nucleic acid bound to the probe material.

Abstract: The present invention provides a centrifugal particle separation and detection device, which can separate and detect particles according to the particle size. The centrifugal particle separate and detection device is practiced with a centrifuge to separate particles, and can be applied to cancer cell detection, blood lymphocyte isolation, tissue engineering, polynucleotide hybridization, microorganism separation and detection and fine chemical purification.

Abstract: Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.

Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.

Abstract: Molecules or particle having a hydrodynamic radius as small as 3.5 nm can be trapped using a double-nanohole structure defined in a metal film or other metallic layer. Application of a suitable optical radiation flux to the double-nanohole structure can provide a folding and/or binding of protein molecules that can be identified based on changes in optical transmission. Varying nanohole transmissions can thus be associated with trapping, binding and unfolding of biological particles. The double-nanohole defines cusps, but such cusps can be defined in other ways as well.

Abstract: The invention concerns the field of detecting and quantifying misfolded proteins/peptides. In particular the detection and quantification of misfolded proteins/peptides in body fluids, on cell surfaces of humans and mammals, the detection of misfolded proteins/peptides in reagents to be tested for scientific research and/or diagnostic use and in pharmaceutical medication or their additives and it concerns as well the removal of misfolded proteins/peptides from reagents to be tested for scientific research and/or for diagnostic purposes and from pharmaceutical medication or their additives.

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

Abstract: According to an example, an apparatus for performing spectroscopy includes a structure having an opening. The apparatus also includes a plurality of surface-enhanced Raman spectroscopy (SERS) elements positioned within the structure and a porous membrane covering the opening and the plurality of SERS elements. The porous membrane is to allow a predetermined analyte to reach the SERS elements while substantially preventing other analytes from reaching the SERS elements.

Abstract: A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.

Abstract: A biosensor device that includes a module defining at least one microfluidic channel, an optical waveguide exposed along at least a portion of its length to fluid flow within the microfluidic channel, where a surface of the optical waveguide being prepared to bind a target biomarker, and an excitation source to couple an excitation wavelength of light into the optical waveguide. The device also includes a sensor for detecting emission light from the optical waveguide at an emission wavelength characteristic of binding of the target biomarker. Particular devices include multiple waveguides in multiple microfluidic channels. Particular devices include microfluidic channels with serpentine bump structures that aid molecular transport.

Abstract: An analysis device for performing analysis about a substance by using an analysis chip having therein a reaction area that reacts with the substance is provided. The analysis device includes: a control section in contact with the analysis chip; a first sensor for measuring an ambient temperature of the analysis chip; a second sensor for measuring the temperature at a contact area of the control section in contact with the analysis chip, and a control circuit for performing feedback control of the control section by finding a temperature of the contact area for achieving a desired temperature of the reaction area based on the ambient temperature measured by the first sensor and a temperature gradient between a position at which the ambient temperature is measured and the reaction area, setting the found temperature as a target value and setting the temperature detected by the second sensor as an output value.

Abstract: A sensor system for detecting a chemical or biological species includes a sensing element and a bias and measurement circuit. The sensing element includes nanochannels having an outer surface functionalized for interaction with the species to create a surface potential, and each having a sufficiently small cross section to exhibit a shift of differential conductance into a negative bias operating region by a shift amount dependent on the surface potential. The bias and measurement circuit applies a bias voltage across two ends of the nanochannels sufficiently negative to achieve a desired dependence of the differential conductance on the surface potential. The dependence has a steeply sloped region of high amplification substantially greater than a reference amplification at a zero-bias condition, thus achieving relatively high signal-to-noise ratio. The bias and measurement circuit converts the measured differential conductance into a signal indicative of presence or activity of the species.

Abstract: A method and apparatus for conducting the rapid pyrolysis of peptides, proteins, polymers, and biological materials. The method can be carried out at atmospheric pressures and takes only about 5 to 30 seconds. The samples are cleaved at the C-terminus of aspartic acid. The apparatus employs a probe on which the sample is heated and digested components analyzed.

Abstract: A SAW sensor includes a substrate, first and second IDT on the substrate and a detector portion between the first and second IDT. The first IDT generates a surface acoustic wave in response to a high-frequency signal input. The second IDT receives the SAW from the first IDT, and converts the SAW to an electric signal and outputs the electric signal. A one pulse signal, which is input to the first IDT electrode, has an applying time that is at least as long as a delay time of a surface acoustic wave at the detector less an arrival time of a direct wave attributable to electromagnetic coupling between the first IDT and the second IDT electrode, and that is less than an arrival time at the second IDT of a third response wave of the surface acoustic wave from the first IDT.

Abstract: The present invention relates to novel lateral flow devices using two dimensional features, preferably, uniform two dimensional test and control features, to provide flow control information, to function as an internal control and/or to provide internal calibration information for the test device. The present invention also relates to methods for using the lateral flow devices to provide flow control information, to function as an internal control and/or to provide internal calibration information for the test device.

Abstract: A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.

Abstract: The present invention relates to novel lateral flow devices using DNA dendrimers, and the methods for detecting an analyte using the lateral flow devices.

Abstract: Provided are a multiple discrimination device and a method of manufacturing the same. According to the multiple discrimination device, a three-dimensional micro ferromagnetic pattern is optimally designed and arranged to allow a magnetic force applied to a discrimination-target particle to be discriminated to be well controlled to perform discrimination well. The method employs a semiconductor processing technology, thereby precisely manufacturing and allowing mass production.

Abstract: A biosensor includes a flexible foil with an electrode layer positioned on the foil. An adhesive layer is positioned on the foil layer, and a first photo-definable hydrogel membrane is positioned over the electrode layer and the adhesive layer. A second photo-definable hydrogel membrane with an immobilized bio-recognition element is positioned over the first hydrogel membrane in contact with the electrode layer through an opening in the first hydrogel membrane.

Abstract: A bio-molecule detecting device that enables a high-sensitivity measurement is provided. The orientation direction of third complexes included in blood plasma is switched by switching the vibration direction of an orientation control light. The orientation direction of the third complexes is switched between two directions in which the intensities of an electric field, which is generated between two gold nanoparticles included in the third complexes by surface plasmon resonance, are significantly different. Therefore, the intensity of fluorescence generated from the third complexes is significantly changed by the change in the orientation direction of the third complexes.

Abstract: The present disclosure provides a method of detecting a target analyte, by binding the target analyte and a probe with at least one fluorophore to form a fluid composition. The fluid composition is excited within a laser cavity. The method comprises measuring a laser emission from the fluid composition based on an interaction between the target analyte and the probe. In certain aspects, the method provides hybridizing the target analyte and the probe, prior to the exciting. In certain variations, the methods comprise detecting a target analyte by measuring a laser emission from the fluid composition based on an interaction between the target analyte, where the interaction is probe energy transfer, intercalation, hybridization of the target analyte and the probe, or combinations thereof. The energy transfer can include fluorescence resonance energy transfer (FRET), FRET with a molecular-beacon, or cavity-assisted radiative energy transfer, by way of non-limiting example.

Abstract: A fluid analysis cartridge and a method for manufacturing the same are provided. The fluid analysis cartridge includes an inspection unit configured to receive and inspect the fluid sample, a housing comprising at least one supply hole that is configured to supply the fluid sample to the inspection unit, and a filtering unit disposed between the supply hole of the housing and the inspection unit and configured to filter a specific substance present in the fluid sample.

Abstract: Provided are an SPR sensor cell having very excellent detection sensitivity and an SPR sensor. The SPR sensor cell includes a detection unit and a sample mounting portion adjacent to the detection unit. The detection unit includes an under clad layer, a core layer provided so that at least a part thereof is adjacent to the under clad layer, and a metal layer covering the core layer. The core layer has a refractive index of 1.43 or less and an absorption coefficient of 9.5×10?2 (mm?1) or less.

Abstract: Methods, kits and devices for detecting antineoplastic drug contamination of a surface are provided according to aspects of the present invention. According to aspects of the invention, methods for detecting antineoplastic drug contamination of a surface include providing a wetting solution compatible with the antineoplastic drug and formulated to promote release of the drug from the surface to be assayed; providing a solid matrix for reversible absorption of the antineoplastic drug; contacting the solid matrix with the wetting solution, generating an assay matrix; contacting the assay matrix and the surface, generating a surface sample; contacting the surface sample with a volume of wetting solution, generating a fluid test sample; and quantifying the antineoplastic drug in the fluid test sample by lateral flow assay to produce an assay result, thereby detecting antineoplastic drug contamination of the surface.

Abstract: Provided are an SPR sensor cell having very excellent detection sensitivity and an SPR sensor. The SPR sensor cell includes: a detection unit; and a sample mounting portion adjacent to the detection unit. The detection unit includes an under clad layer, a core layer provided so that at least a part thereof is adjacent to the under clad layer, and a metal layer covering the core layer. The core layer contains 35 wt % or more of a halogen.

Abstract: Severe traumatic brain (TBI) injuries are often associated with long-term and disabling consequences. Prognosis and chronic treatment planning following severe TBI remain challenging. The discovery of specific brain biomarkers could create new opportunities for more accurate clinical assessments identifying groups that may experience better outcomes when exposed to an intervention. The present invention provides a method of detection of Microtubule-associated protein-2 (MAP-2), a marker of dendritic damage, in a biological sample of survivors after TBI and evaluates the recovery of the patient, including an improvement in cognitive abilities and function.

Abstract: A process for a pre-concentration unit including: a sorbent material coated passage-way having an entrance for receiving a hydrocarbon-containing groundwater sample and for pre-concentrating the hydrocarbons in a hydrocarbon-containing groundwater sample by successive sorption/desorption cycles, and having an exit for discharging the pre-concentrated hydrocarbons; a heating unit for heating the sorbent material coated passage-way; and; an array of shear horizontal-surface acoustic wave sensors with coatings adapted for detecting and quantifying the pre-concentrated hydrocarbons, disposed at the exit of the sorbent material coated passage-way; and a housing for enclosing the pre-concentration unit and the array of shear horizontal-surface acoustic wave sensors, adapted for continuous use at a body of hydrocarbon-containing groundwater, and coupled to mathematical methods for generating concentrations of specific analytes from both transient and steady-state signals.

Abstract: A label-free RF MEMS-based biosensor is described for detecting biomarkers in a given environment. The biosensor is capable of sensing the presence of biomarkers by exploiting both its mechanical and electrical characteristics. In addition, the method employed for detecting mechanical deflections due to antigen-antibody binding uses a simple electrical circuitry which allows the sensor to be used at any location and time. Such a sensor, when placed in a matrix like structure allows for the detection of multiple biomarkers simultaneously.

Abstract: A portable magnetic resonance system includes a permanent magnet, a nuclear magnetic resonance probe, and control electronics. The control electronics are configured to transmit to the probe a magnetic resonance excitation signal having an excitation frequency f, receive from the probe a magnetic resonance measurement signal, detect in the magnetic resonance measurement signal a magnetic resonance frequency f0, and automatically adjust the excitation frequency f until the difference between the excitation frequency and the magnetic resonance frequency is approximately equal to a target offset.

Abstract: An apparatus for detecting a selected liquid contaminant includes a material holder and a reactive material held by the material holder. The reactive material absorbs a selected liquid contaminant with a measurable change reaction occurring as a result of such absorption. A signal generator is provided for generating a signal upon the measurable change reaction exceeding a preset threshold.

Abstract: Applying an ultrasound to a sample solution by an ultrasound application unit and pressing a fluorescent labeling substance in the sample solution against a metal layer. Under this state, applying an excitation light to the interface between a dielectric plate and the metal layer at a specific angle greater than or equal to a total reflection angle by a light application unit and forming a surface plasmon enhanced optical field area on the metal layer, thereby exciting the fluorescent labeling substance. Detecting fluorescence generated from the fluorescent labeling substance through the excitation by a fluorescence detection unit.

Abstract: A rapid, efficient and convenient method to detect one or more biological entities on a blotting membrane is provided. The detection can relate to the position, nature or amount of the biological substance on one or more membranes. The invention method involves a pressure assisted regiment (such as vacuum or positive gas pressure) for the supply and removal of reagents and permits washing of the contaminants from substances embedded in the membrane to be detected using very low volumes of liquid. This method enables completion of the blocking, washing and antibody binding steps in about 30 minutes without comprising blot quality. In another aspect, the invention is directed to an apparatus useful in conducting the method of the invention.

Abstract: Ionic liquid (IL)-mediated sol-gel hybrid organic-inorganic materials present enormous potential for effective use in analytical microextraction. One obstacle to materializing this prospect arises from high viscosity of ILs significantly slowing down sol-gel reactions. A method was developed which provides phosphonium-based, pyridinium-based, and imidazolium-based IL-mediated advanced sol-gel organic-inorganic hybrid materials for capillary microextraction. Scanning electron microscopy results demonstrate that ILs can serve as porogenic agents in sol-gel reactions. IL-mediated sol-gel coatings prepared with silanol-terminated polymers provided up to 28 times higher extractions compared to analogous sol-gel coatings prepared without any IL in the sol solution.

Abstract: This invention relates to a method of making lead-free piezoelectric ceramic films. Specifically, the invention is directed to a method for fabricating lead-free piezoelectric free standing films having enhanced piezoelectric properties. The films may be used for a number of applications including incorporation in microelectronic devices such as energy harvesting devices and sensor technologies.

Abstract: The invention pertains to a disposable cartridge suitable for use in detecting the presence of analytes in fluid samples. The cartridge is designed to provide “lab on a chip” capabilities and comprises a plurality of sample storage reservoirs and analytical compartments/channels. A valve system based on valve disks aids in securely transferring samples from storage reservoirs to analytical channels and detectors with minimal use of common channels, which reduces risk of cross-contamination.

Abstract: Methods and systems are disclosed for rapid detection of cerebrospinal fluid (CSF) in a sample. In some embodiments, the methods comprise depleting a biological sample of beta-1 transferrin by contacting the sample with a sialic acid-specific lectin bound to a solid support, followed by subjecting the beta-1 transferrin-depleted sample to a lateral flow immunoassay. The methods can be used to detect CSF comprised by a sample in under one hour. Furthermore, the methods can detect CSF in a biological sample such as a plasma sample of a volume as small as about 10 ?l.

Abstract: The invention relates to the detection and quantification of 1-benzylpiperazine and its metabolites. The invention is underpinned by novel polyclonal antibodies with unique binding properties which enable immunoassay methods and kits for various applications.

Abstract: In one aspect, a diagnostic test system includes a housing, a reader, and a data analyzer. The housing includes a port for receiving a test strip. The reader obtains separable light intensity measurements from localized regions of an area of the detection zone exposed for optical inspection, wherein each of the localized regions is characterized by at least one surface dimension smaller than the first dimension. The data analyzer identifies ones of the light intensity measurements obtained from the at least one test region and computes at least one parameter from the identified ones of the light intensity measurements. In another aspect, the reader obtains a respective set of light intensity measurements from each of multiple corresponding regions of the exposed surface area of the detection zone, and the data analyzer computes at least one parameter from at least one of the sets of light intensity measurements.

Abstract: A system, and its associated methodology, for detecting cTnl includes a patient sample such as whole blood, serum or plasma, anti-cTnl antibodies conjugated with ligand-capped metallic nanoparticles, and a spectrophotometer operable to detect a shift of wavelength in light resulting from binding of cTnl to the anti-cTnl antibodies conjugated with ligand-capped metallic nanoparticles. By measuring changes in Localized Surface Plasmon Resonance of the anti-cTnl antibodies conjugated with ligand-capped metallic nanoparticles the presence of cTnl can be detected.

Abstract: A sensor strip apparatus includes: a top plate having an entrance opening downward and a joint formed downward; a pad section including a support having a window opening downward, a reaction pad attached to the window of the support and reacting with a specimen, first and second hemolysis inhibition pads attached to the reaction pad to filter hemocytes from the specimen, a specimen pad attached to the first and second hemolysis inhibition pads to diffuse the specimen crosswise, and an adhesive film attached to the support around the first and second hemolysis inhibition pads to increase adhesion strength of the specimen pad; and a bottom plate having a second joint forcibly coupled with to the joint of the top plate, and a window configured to indentify the reaction pad through the window of the support.

Abstract: A position adjustment method for a movable unit in a sample analyzer which includes: a measurement section which causes a movable unit to operate in order to measure a sample; and a communication section for performing communication with outside, includes: a terminal screen displaying step of causing a portable terminal device to display a position adjustment screen for accepting an input for changing a position of the movable unit, the portable terminal device configured to be able to perform communication with the communication section; and a movement executing step of causing the measurement section to execute a movement of a corresponding movable unit in accordance with the input for changing the position received by the communication section.

Abstract: Provided herein are compositions, kits, methods and devices for cyanide detection, particularly for cyanide detection in biological samples such as whole blood. The method comprises (1) contacting a sample with a cobinamide conjugate comprising a cobinamide moiety and a carrier; and (2) measuring the absorbance of light by the cobinamide conjugate. The present disclosure provides field-deployable cyanide detection methods, compositions, kits and devices, which provide rapid, accurate readout at the point of contact. Further provided herein is a method for determining exposure of a subject to cyanide.