Dicarboxylic Acid Having Four Or Less Carbon Atoms (e.g., Fumaric, Maleic, Etc.) Patents (Class 435/145)
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Patent number: 8343752Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.Type: GrantFiled: September 26, 2011Date of Patent: January 1, 2013Assignee: Verdezyne, Inc.Inventors: Stephen Picataggio, Tom Beardslee
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Publication number: 20120329095Abstract: Provided is a method of producing succinic acid in which a microorganism having a succinic acid-producing ability is allowed to react with a sugar, the method being characterized in that the ratio of the oxygen transfer rate to the succinic acid production rate (mmol-O2/mol-SA) is 0.1 to 240 and that the doubling time of the microorganism during the reaction is not shorter than 40 hours.Type: ApplicationFiled: September 7, 2012Publication date: December 27, 2012Applicant: MITSUBISHI CHEMICAL CORPORATIONInventors: Katsunori YOSHIKAWA, Daisuke KIDO, Makoto MURASE
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Patent number: 8334119Abstract: The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pII, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.Type: GrantFiled: January 11, 2012Date of Patent: December 18, 2012Assignee: William Marsh Rice UniversityInventor: Ramon Gonzalez
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Publication number: 20120309065Abstract: Strict anaerobic thermophilic bacterium belonging to the group of Thermoanaerobacter italicus subsp. marato subsp. nov. and mutants and derivatives thereof. The bacterium is particularly suitable for the production of fermentation products such as ethanol, lactic acid, acetic acid and hydrogen from lignocellulosic biomass.Type: ApplicationFiled: December 21, 2010Publication date: December 6, 2012Applicant: BIOGASOL IPR APSInventors: Thomas Kvist, Marie Just Mikkelsen, Rasmus Lund Andersen
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Publication number: 20120288907Abstract: A method is disclosed for restoring a Glu+ phenotype to a PTS?/Glu? bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.Type: ApplicationFiled: May 14, 2012Publication date: November 15, 2012Applicant: DANISCO US INC.Inventors: Marguerite A. Cervin, Philippe Soucaille, Fernando Valle, Gregory M. Whited
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Publication number: 20120276594Abstract: The present invention relates to cellobiohydrolase variants having improved thermostability and/or thermoactivity in comparison to wild-type Myceliophthora thermophila CBH2b.Type: ApplicationFiled: April 27, 2012Publication date: November 1, 2012Applicant: Codexis, Inc.Inventors: Rama Voladri, Xiyun Zhang, Sachin Patil, David Elgart, Gregory Miller, Louis Clark, Kui Chan
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Publication number: 20120270267Abstract: The present invention relates to alpha-amylase variants, polynucleotides encoding the variants and nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.Type: ApplicationFiled: January 4, 2011Publication date: October 25, 2012Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20120264176Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: January 4, 2011Publication date: October 18, 2012Applicant: Novozymes A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20120264171Abstract: The present invention relates to variants of a parent alpha-amylase, the variant having improved stability or activity at low calcium conditions or at low pH.Type: ApplicationFiled: January 4, 2011Publication date: October 18, 2012Applicant: NOVOZYMES A/SInventor: Carsten Andersen
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Publication number: 20120258497Abstract: The present invention relates to alpha-amylase variants, polynucleotides encoding the variants and nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.Type: ApplicationFiled: January 4, 2011Publication date: October 11, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20120258504Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: ApplicationFiled: June 4, 2012Publication date: October 11, 2012Applicant: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Publication number: 20120244587Abstract: A fermentation process includes contacting a carbohydrate source with a microorganism in an aqueous fermentation broth to form a fermentation product which is a salt or a product with a boiling point above the boiling point of water. The fermentation process is carried out at a pressure which is below atmospheric pressure and at least at the value where the reaction medium is at its boiling point at the fermentation temperature. Water is evaporated and removed from the reactor during the fermentation in an amount which is at least 20% of the volume of liquid present in the reactor at the start of the fermentation. A fermentation process at reduced pressure while removing a substantial amount of water removes a surplus of water in the system, ensures removal of reaction heat, and may lead to improved fermentation quality.Type: ApplicationFiled: October 14, 2010Publication date: September 27, 2012Applicant: Purac Biochem BVInventors: Jan Van Breugel, Willem Jacob Groot, Peter Paul Jansen
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Publication number: 20120238722Abstract: The present invention relates to a process for recovering succinic acid in crystal form from a fermentation broth comprising succinic acid, comprising the steps of a) bringing the fermentation broth to a pH of between 1 and 4, b) crystallizing the succinic acid from the fermentation broth to form succinic acid crystals, c) dissolving the succinic acid crystals at a temperature of between 30 and 90 degrees Celsius to form an aqueous solution comprising dissolved succinic acid, d) crystallizing the succinic acid from the solution to recover succinic acid in crystal form. The invention further relates to succinic acid in crystal form, comprising a sugar content of 1 to 100 ppm and a nitrogen content of 1 to 80 ppm.Type: ApplicationFiled: November 19, 2010Publication date: September 20, 2012Applicants: ROQUETTE FRERES SA, DSM IP ASSETS B.V.Inventors: Maarten Job Van De Graaf, Fredoen Vallianpoer, Guillaume Fiey, Loic Delattre, Elisabeth Agnes Michèle Schulten
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Publication number: 20120237979Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 30, 2010Publication date: September 20, 2012Applicant: NOVOZYMES A/SInventors: Junxin Duan, Kirk Matthew Schnorr, Wenping Wu
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Publication number: 20120231511Abstract: Disclosed is a preparation method for bio-fuel materials and bio-chemicals comprising the following steps: preparing a medium comprising fermentation waste generated in an alcohol production process; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated. More specifically, the preparation method for bio-fuel materials and bio-chemicals comprises the following steps: fermenting hexoses from a mixture of pentoses and hexoses to produce an ethanol fermentation broth; separating and purifying the ethanol fermentation broth; preparing a medium comprising the fermentation waste produced in the separation and purification step; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated.Type: ApplicationFiled: September 13, 2010Publication date: September 13, 2012Applicants: CHANGHAE ETHANOL CO., LTD., GS CALTEX CORPORATIONInventors: Julia Lee, Hyo Hak Song, Moon Ho Ueom, Jung Hee Cho, Do Young Seung, Gi-Wook Choi, Se-Kwon Moon, Sung Hoon Park, Yule Kim, Min Hee Han
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Publication number: 20120225461Abstract: This invention relates to succinic acid production from renewable feedstock using microbial biocatalysts genetically modified to produce succinic acid in commercially significant quantities. More specifically, this invention relates to the genetic manipulations in the pathway of carbon from renewable feedstock to succinic acid.Type: ApplicationFiled: November 18, 2010Publication date: September 6, 2012Applicant: MYRIANT CORPORATIONInventors: Sudhanshu Dole, R. Rogers Yocum
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Publication number: 20120225452Abstract: A novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence, and a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60° C.Type: ApplicationFiled: June 20, 2011Publication date: September 6, 2012Applicant: SHAN DONG UNIVERSITYInventors: Xiaoming Bao, Yu Shen, Ruilei GE
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Publication number: 20120225451Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.Type: ApplicationFiled: September 2, 2011Publication date: September 6, 2012Applicant: Technische Universiteit DelftInventors: Aaron Adriaan WINKLER, Sipko Maarten Kuyper, Wilhelmus Theodorus Antonius Maria De Laat, Johannes Pieter Van Dijken, Jacobus Thomas Pronk
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Publication number: 20120219997Abstract: The present invention refers to the new Escherichia coli strains denominated JU15, JU15A, LL26 and MS04 and their derivatives that produce metabolites, particularly D-lactate, L-lactate or ethanol, with high yield and selectivity from a wide variety of carbon sources, such as culture media with a high xylose content (as the main carbon source) and, in particular, media formulated with hydrolyzed vegetables, such as sugarcane bagasse, agave bagasse and fast-growing grasses, and a wide variety of agricultural and industrial wastes, such as whey or forestry wastes, celluloses, grasses, agave bagasse, paper wastes, shavings and sawdust, shrubs and generally any material derived from lignocellulose. These strains use the production of the metabolite of interest (especially D-lactate, L-lactate or ethanol) as the only way of regenerating the reducing power.Type: ApplicationFiled: August 6, 2010Publication date: August 30, 2012Inventors: Alfredo Martinez Jimenez, Guillermo Gosset Lagarda, Georgina Teresa Hernandez Chavez, Gerardo Huerta Beristain, Berenice Trujillo Martinez, Jose Utrilla Carreri
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Publication number: 20120219999Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: February 28, 2012Publication date: August 30, 2012Applicant: NOVOZYMES, INC.Inventors: Amanda Fischer, Stephen Brown, Sheryl Luttringer
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Publication number: 20120220000Abstract: This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.Type: ApplicationFiled: November 17, 2010Publication date: August 30, 2012Inventors: Wei Gong, Sudhanshu Dole, Tammy Grabar, Andrew Chirstopher Collard, Janice G. Pero, R. Rogers Yocum
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Publication number: 20120214209Abstract: The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate.Type: ApplicationFiled: April 27, 2012Publication date: August 23, 2012Applicant: Danisco US Inc.Inventors: Gopal K. Chotani, Manoj Kumar, Jeff P. Pucci, Karl J. Sanford, Jayarama K. Shetty
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Patent number: 8247201Abstract: An organic acid is produced by allowing a bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an organic acid and has been modified so that the phosphoenolpyruvate carboxykinase activity is enhanced, which is selected from Enterobacter, Pantoea, Erwinia, Klebsiella and Raoultella bacteria, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.Type: GrantFiled: June 3, 2010Date of Patent: August 21, 2012Assignee: Ajinomoto Co., Inc.Inventors: Yoshinori Tajima, Keita Fukui, Kenichi Hashiguchi
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Publication number: 20120208231Abstract: The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and/or convert L-arabinose into L-ribulose, and/or xylulose 5-phosphate and/or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.Type: ApplicationFiled: January 6, 2012Publication date: August 16, 2012Applicant: DSM IP ASSETS B.V.Inventors: Antonius Jeroen Adriaan VAN MARIS, Jacobus Thomas PRONK, Hendrik Wouter WISSELINK, Johannes Pieter VAN DIJKEN, Aaron Adriaan WINKLER, Johannes Hendrik DE WINDE
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Patent number: 8241879Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.Type: GrantFiled: September 26, 2011Date of Patent: August 14, 2012Assignee: Verdezyne, Inc.Inventors: Stephen Picataggio, Tom Beardslee
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Publication number: 20120196338Abstract: Methods and compositions are provided for improving the production of products, such as fuel products like ethanol, in microorganisms. In particular, methods and compositions are described for improving ethanol production utilizing genes identified in Clostridium phytofermentans.Type: ApplicationFiled: April 14, 2011Publication date: August 2, 2012Applicants: Qteros, Inc., University of MassachusettsInventors: Jeffrey Blanchard, Susan Leschine, Elsa Petit, John Fabel, Matthias Schmalisch
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Publication number: 20120183993Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.Type: ApplicationFiled: October 5, 2010Publication date: July 19, 2012Applicant: DSM IP ASSETS B.V.Inventors: Johannes Petrus Smits, Elisabeth Maria Gierveld, Fop Cornelis Van Der Hor
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Publication number: 20120165569Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.Type: ApplicationFiled: November 14, 2008Publication date: June 28, 2012Inventors: René Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
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Publication number: 20120164703Abstract: Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.Type: ApplicationFiled: December 16, 2011Publication date: June 28, 2012Applicant: Michigan Biotechnology InstituteInventors: Jian YI, Susanne Kleff, Michael V. Guettler
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Publication number: 20120156735Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: ApplicationFiled: June 15, 2011Publication date: June 21, 2012Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20120149079Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: February 24, 2012Publication date: June 14, 2012Applicant: NOVOZYMES, INC.Inventors: Amanda Fischer, Debbie Yaver
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Patent number: 8192965Abstract: Method for producing itaconic acid in yeast cells using glycerol as the substrate. The yeast cells express cis-aconitic acid decarboxylase and optionally, citrate synthase and/or aconitase at high levels.Type: GrantFiled: August 25, 2009Date of Patent: June 5, 2012Assignee: Industrial Technology Research InstituteInventors: Jia-Hung Wang, Shu-Hsien Tsai, Kelly Teng
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Publication number: 20120129233Abstract: An organic acid is produced by allowing a bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an organic acid and has been modified so that the phosphoenolpyruvate carboxykinase activity is enhanced, and the glucose phosphotransferase activity is decreased, which is selected from Escherichia, Enterobacter, Pantoea, Erwinia, Klebsiella and Raoultella bacteria, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid.Type: ApplicationFiled: November 21, 2011Publication date: May 24, 2012Inventors: Yoshinori Tajima, Yoko Yamamoto
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Publication number: 20120115186Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.Type: ApplicationFiled: July 6, 2010Publication date: May 10, 2012Applicant: DSM IP ASSETS B.V.Inventors: Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
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Publication number: 20120114655Abstract: The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.Type: ApplicationFiled: January 16, 2012Publication date: May 10, 2012Applicant: University of Florida Research Foundation, Inc.Inventors: JAMES F. PRESTON, III, Virginia Chow, Guang Nong, John D. Rice, Franz J. St. John
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Publication number: 20120107885Abstract: The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pH, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol.Type: ApplicationFiled: January 11, 2012Publication date: May 3, 2012Applicant: William Marsh Rice UniversityInventor: Ramon GONZALEZ
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Patent number: 8158395Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: GrantFiled: June 21, 2011Date of Patent: April 17, 2012Assignee: Novozymes, Inc.Inventors: Amanda Fischer, Debbie Yaver
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Patent number: 8143036Abstract: Genetically modified microorganisms that produce itaconic acid at high yields and uses thereof.Type: GrantFiled: May 11, 2009Date of Patent: March 27, 2012Assignee: Industrial Technology Research InstituteInventors: James C. Liao, Pei-Ching Chang
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Publication number: 20120058530Abstract: This invention relates to the biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) depressed by genetic modifications and a genetically-inactivated phosphotransferase system.Type: ApplicationFiled: April 2, 2010Publication date: March 8, 2012Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION INC.Inventors: Xueli Zhang, Kaemwich Jantama, Jonathan C. Moore, Laura R. Jarboe, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Patent number: 8129154Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.Type: GrantFiled: June 17, 2009Date of Patent: March 6, 2012Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Priti Pharkya
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Publication number: 20120052536Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, methods are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.Type: ApplicationFiled: July 15, 2011Publication date: March 1, 2012Applicant: XYLECO, INC.Inventors: Marshall MEDOFF, Thomas Craig MASTERMAN, Seul-a BAE, Kelly WALLICK
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Publication number: 20120052540Abstract: The present invention relates to novel stress-resistant bacteria and the uses thereof. More specifically, the invention relates to isolated stress-resistant bacteria having advantageous properties for the production of organic acids or alcohols in various culture conditions. The invention also relates to methods of producing organic acids or alcohols using said bacteria, particularly from biomass.Type: ApplicationFiled: May 12, 2010Publication date: March 1, 2012Applicants: DEINOVE, UNIVERSITE MONTPELLIER I, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Jacques Biton, Cathy Isop
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Patent number: 8119377Abstract: Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant Actinobacillus succinogenes that has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.Type: GrantFiled: December 16, 2005Date of Patent: February 21, 2012Assignee: Michigan Biotechnology InstituteInventors: Jian Yi, Susanne Kleff, Michael V. Guettler
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Publication number: 20120040423Abstract: The present invention provides a method for providing a target chemical, comprising: providing a cellulose-derived slurry comprising fermentable saccharides in one or several containers; subjecting cellulose-derived slurry from said one or several containers to separation to provide a liquid fraction comprising part of said fermentable saccharides from said cellulose-derived slurry and a suspended solids fraction, which is returned to said container; transferring said liquid fraction to a fermentor; fermenting fermentable saccharides of said liquid fraction in said fermentor to provide a fermented liquid comprising said target chemical; and returning said fermented liquid to said one or several containers.Type: ApplicationFiled: April 28, 2010Publication date: February 16, 2012Inventor: Hans Grundberg
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Publication number: 20120040422Abstract: The present invention relates to a process for the production of a dicarboxylic acid and ethanol which comprises fermenting a genetically modified yeast in a suitable fermentation medium under anaerobic conditions at a pH value of between 1 and 5 and producing the dicarboxylic acid and ethanol.Type: ApplicationFiled: March 23, 2010Publication date: February 16, 2012Applicant: DSM IP ASSET B.V.Inventors: Mickel Leonardus August Jansen, Maarten Job Van De Graaf, René Verwaal
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Publication number: 20120034659Abstract: Embodiments of the present disclosure relate to a process for producing downstream products, such as fermentable sugars and end products, from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.Type: ApplicationFiled: August 5, 2011Publication date: February 9, 2012Applicant: Danisco US Inc.Inventors: Martien H. Bergsma, Gopal K. Chotani, William A. Cuevas, Gang Duan, Sung Ho Lee, Ying Qian, Vivek Sharma, Jayarama K. Shetty, Bruce A. Strohm, Paula Johanna Maria Teunissen, Hongxian Xu
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Publication number: 20120034648Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: ApplicationFiled: March 5, 2009Publication date: February 9, 2012Inventors: Paul Klaassen, Jan Metske Van Der Laan, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom
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Publication number: 20120021474Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.Type: ApplicationFiled: September 26, 2011Publication date: January 26, 2012Applicant: VERDEZYNE, INC.Inventors: Stephen PICATAGGIO, Tom Beardslee
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Publication number: 20120021473Abstract: Processes for making SA from either a clarified DAS-containing fermentation broth or a clarified MAS-containing fermentation broth that include distilling the broth under super atmospheric pressure at a temperature of >100° C. to about 300° C. to form an overhead that comprises water and ammonia, and a liquid bottoms that includes SA, and at least about 20 wt % water; cooling the bottoms to a temperature sufficient to cause the bottoms to separate into a liquid portion and a solid portion that is substantially pure SA; and separating the solid portion from the liquid portion. A method also reduces the broth distillation temperature and pressure by adding an ammonia separating and/or water azeotroping solvent to the broth.Type: ApplicationFiled: September 16, 2011Publication date: January 26, 2012Applicant: BIOAMBER S.A.S.Inventors: Olan S. Fruchey, Brian T. Keen, Brooke A. Albin, Bernard D. Dombek, Nye A. Clinton, Paul R. Zitzelsberger, Matthew B. Brumley
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Publication number: 20120015415Abstract: The present invention provides a method for producing succinic acid using a yeast belonging to the genus Yarrowia, which has been modified to reduce activity of succinate dehydrogenase in said yeast.Type: ApplicationFiled: July 20, 2011Publication date: January 19, 2012Inventors: Sergey Pavlovich Sineoky, Tigran Vladimirovich Yuzbashev, Evgenia Yurievna Yuzbasheva, Tatyana Ivanova Sobolevskaya, Ivan Aleksandrovich Laptev, Tatyana Vladimirovna Vibornaya