Dicarboxylic Acid Having Four Or Less Carbon Atoms (e.g., Fumaric, Maleic, Etc.) Patents (Class 435/145)
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Publication number: 20140096287Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: April 3, 2014Inventors: Kristian Krogh, Paul Harris
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Publication number: 20140093925Abstract: Recombinant microorganisms that co express enzymatic glucose-6-phosphate dehydrogenase and malate dehydrogenase are generated to produce organic acids.Type: ApplicationFiled: October 2, 2013Publication date: April 3, 2014Applicant: THE MICHIGAN BIOTECHNOLOGY INSTITUTEInventors: Michael Guettler, Robert Hanchar, Susanne Kleff, Sachin Jadhav
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Patent number: 8685703Abstract: A coryneform bacterium transformant prepared by transferring an exogenous gene which encodes a protein having a sugar transporter function into a coryneform bacterium capable of utilizing D-xylose.Type: GrantFiled: June 10, 2009Date of Patent: April 1, 2014Assignee: Research Institute of Innovative Technology for the EarthInventors: Hideaki Yukawa, Masayuki Inui
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Publication number: 20140087433Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce an intermediate or product, e.g., by fermentation.Type: ApplicationFiled: November 21, 2012Publication date: March 27, 2014Applicant: XYLECO, INC.Inventor: Xyleco, Inc.
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Patent number: 8679801Abstract: The invention relates to a nucleic acid sequence encoding an Aspergillus mitochondrial tricarboxylic acid transporter that can be used in the production of itaconic acid in micro-organisms. Preferably said transporter protein is the protein encoded by the nucleic acid which is located on a chromosome segment of A. terreus that also harbors other genes involved in the itaconic acid biosynthesis and the lovastatin biosynthesis. Also, vectors, hosts and transformed micro-organisms are part of the invention.Type: GrantFiled: February 16, 2009Date of Patent: March 25, 2014Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek TNOInventors: Peter Jan Punt, Maria Johanna Van Der Werf
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Patent number: 8673598Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.Type: GrantFiled: February 12, 2010Date of Patent: March 18, 2014Assignee: BASF SEInventors: Hartwig Schroder, Stefan Haefner, Gregory Von Abendroth, Rajan Hollmann, Aline Raddatz, Hansgeorg Ernst, Hans Gurski
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Publication number: 20140075601Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: October 28, 2013Publication date: March 13, 2014Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Publication number: 20140075603Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: March 13, 2014Inventors: Lan Tang, Ye Liu, Junxin Duan, Wenping Wu, Randall Kramer
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Publication number: 20140065680Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.Type: ApplicationFiled: November 4, 2013Publication date: March 6, 2014Applicant: DSM IP ASSETS B.V.Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
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strain, mutagenesis and screening methods thereof, and methods of fermenting to produce fumaric acid
Patent number: 8663972Abstract: A rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named as Rhizopus oryzae ME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying.Type: GrantFiled: April 16, 2011Date of Patent: March 4, 2014Inventors: He Huang, Shuang Li, Qing Xu, Zhen Gao -
Patent number: 8663956Abstract: Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid.Type: GrantFiled: August 17, 2012Date of Patent: March 4, 2014Assignee: Novozymes, Inc.Inventors: Sarah McFarland, Stephen Brown, Sheryl Luttringer
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Publication number: 20140051133Abstract: Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.Type: ApplicationFiled: May 10, 2013Publication date: February 20, 2014Applicant: BUTAMAX ADVANCED BIOFUELS, LLCInventors: Sridhar Govindarajan, Yougen Li, Der-Ing Liao, Daniel P. O'Keefe, Jeremy Stephen Minshull, Steven Cary Rothman, Alexander Vincent Tobias
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Publication number: 20140051119Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: June 10, 2013Publication date: February 20, 2014Applicant: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20140051130Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: October 11, 2013Publication date: February 20, 2014Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Publication number: 20140045230Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.Type: ApplicationFiled: October 21, 2013Publication date: February 13, 2014Applicant: DSM IP ASSETS B.V.Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
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Patent number: 8647843Abstract: Provided is a method of producing succinic acid in which a microorganism having a succinic acid-producing ability is allowed to react with a sugar, the method being characterized in that the ratio of the oxygen transfer rate to the succinic acid production rate (mmol-O2/mol-SA) is 0.1 to 240 and that the doubling time of the microorganism during the reaction is not shorter than 40 hours.Type: GrantFiled: September 7, 2012Date of Patent: February 11, 2014Assignee: Mitsubishi Chemical CorporationInventors: Katsunori Yoshikawa, Daisuke Kido, Makoto Murase
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Publication number: 20140038229Abstract: Provided are methods for generating a microorganism with improved ability to convert glycerol to succinic acid. These methods comprise combining a microorganism with ability to convert glycerol to succinic acid and a medium containing glycerol to produce a fermentation mixture; allowing fermentation to occur such that succinic acid is produced; and assaying for an indication of glycerol metabolism. These latter two steps may be repeated as necessary. Also provided are microorganisms generated in this manner, particularly bacteria deposited at the NCIMB on 13th April 2011 with the Accession Number NCIMB 41825. A method of producing succinic acid from glycerol is also provided, this method comprising: mixing a microorganism according to claim of the invention with a medium comprising glycerol to produce a fermentation mixture; and incubating the fermentation mixture, under conditions that promote fermentation to produce succinic acid, until succinic acid is produced.Type: ApplicationFiled: April 20, 2012Publication date: February 6, 2014Applicant: THE UNIVERSITY OF MANCHESTERInventor: Constantinos Theodoropoulos
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Publication number: 20140030763Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: June 20, 2013Publication date: January 30, 2014Inventor: Marshall Medoff
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Publication number: 20140031587Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyses the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.Type: ApplicationFiled: October 2, 2013Publication date: January 30, 2014Applicant: DSM IP Assets B.V.Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
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Publication number: 20140030778Abstract: The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.Type: ApplicationFiled: October 3, 2013Publication date: January 30, 2014Applicant: BASF SEInventors: EDZARD SCHOLTEN, DIRK DAGELE, STEPHAN HAEFNER, HARTWIG SCHRODER
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Publication number: 20140024086Abstract: The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.Type: ApplicationFiled: March 29, 2012Publication date: January 23, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Elliot N. Miller, Xueli Zhang, Lorraine P. Yomano, Xuan Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140018511Abstract: The present invention provides a method of producing a polymer, which comprises the step of performing a polymerization reaction using, as a starting material, an organic acid obtained by allowing a microorganism or a treated cell thereof to act on an organic raw material, wherein said microorganism has an ability to produce an organic acid and has been modified so as to produce less aromatic carboxylic acid as compared to an unmodified strain.Type: ApplicationFiled: September 18, 2013Publication date: January 16, 2014Applicant: MITSUBISHI CHEMICAL CORPORATIONInventors: Shuichi YUNOMURA, Gou Takahashi, Yoshiaki Mori
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Publication number: 20140017737Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 23, 2013Publication date: January 16, 2014Inventor: Mark Wogulis
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Publication number: 20140004560Abstract: A rhizopus oryzae strain, mutagenesis and screening methods thereof, and methods of producing fumaric acid by fermentation. The strain is named as Rhizopus oryzae ME-F13, and deposited in China Center for Type Culture Collection with depository number CCTCC M 2010351. The strain is obtained by physically mutagenizing the original strain ME-F12 through ion injection, culturing the processed bacteria on the solid selective plate containing 2-D-deoxylucose (2-DG) and picking up 2-DG-resistant single colony. The strain is capable of simultaneously saccharifying starchy material and fermenting it to produce fumaric acid. With an improved enzymatic activity, the strain can be directly used to ferment raw starchy materials without needing pre-saccharifying.Type: ApplicationFiled: April 16, 2011Publication date: January 2, 2014Inventors: He Huang, Shuang Li, Qing Xu, Zhen Gao
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Publication number: 20140004578Abstract: The invention generally relates to methods, devices, and microbes involved in performing redox imbalanced fermentations. In one aspect, the invention provides a device that generally includes an electrode and at least one microbe in electron communication with the electrode. The microbe generally can exhibit increased activity of at least one enzyme involved in converting a substrate to a redox imbalanced product. In another aspect, the invention provides a method for performing redox imbalanced fermentation. Generally, the method includes providing a substrate to a microbe under conditions effective for the microbe to metabolize the substrate to a redox imbalanced product. At least one microbe may be in contact with an electrode. In some cases, metabolic conversion of the substrate to the redox imbalanced product can include transferring electrons between the electrode and the microbe.Type: ApplicationFiled: September 13, 2011Publication date: January 2, 2014Inventors: Daniel R. Bond, Jeffrey A. Gralnick, Daniel E. Ross, Jeffrey M. Flynn
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Patent number: 8617859Abstract: The present invention relates to methods of producing C4 dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4 dicarboxylic acid transporter; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, as well as host cells comprising the polynucleotides.Type: GrantFiled: June 2, 2011Date of Patent: December 31, 2013Assignee: Novozymes, Inc.Inventors: Sarah McFarland, Amanda Fischer
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Publication number: 20130337519Abstract: This invention relates to the production of chemicals by fermentation with a microorganism in which the fermentation medium contains the sugar sucrose. As a specific example, succinic acid is produced from a sucrose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize sucrose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and/or a salt of succinic acid) at a commercial scale using feedstocks other than sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of sucrose into the parental strains.Type: ApplicationFiled: December 13, 2011Publication date: December 19, 2013Applicant: MYRIANT CORPORATIONInventors: Sudhandshu Dole, R. Rogers Yocum, Theron Hermann, Xiaohui Yu
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Publication number: 20130337517Abstract: Overlay processes are disclosed for making ethanol that not only increase ethanol conversion but do so in a cost effective manner with a reduction in energy requirements per unit of ethanol production. The processes can provide, if desired, higher organic compound as a co-product with ethanol.Type: ApplicationFiled: June 14, 2013Publication date: December 19, 2013Inventors: Fatemeh RAZAVI-SHIRAZI, Ameen RAZAVI, Norman Louis BALMER
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Patent number: 8597917Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.Type: GrantFiled: October 29, 2012Date of Patent: December 3, 2013Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman, Harrison Medoff
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Publication number: 20130309730Abstract: The present invention relates to novel enzymes and the uses thereof. The invention also relates to methods of producing such enzymes, coding nucleic acid molecules, recombinant cells and methods of modifying biomass from such materials. The invention is particularly suited to degrade biomass and/or to improve biomass degradation. The invention also relates to various applications of the enzymes of the invention for the production of bioenergy (such as bioethanol), as well as in the field of chemistry, paper industry, textile industry and beverage industry.Type: ApplicationFiled: November 8, 2011Publication date: November 21, 2013Applicant: DEINOVEInventors: Jean-Paul Leonetti, Jean-Michel Claverie, Nicolas Chabot
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Publication number: 20130309736Abstract: The present application provides genetically modified yeast cell comprising an active malate fermentation pathway and/or an active fumarate fermentation pathway, as well as methods of using these cells to produce malate and/or fumarate.Type: ApplicationFiled: January 25, 2012Publication date: November 21, 2013Applicant: CARGILL, INCORPORATEDInventors: Kenneth R. Finley, Jeanette M. Huryta, Beth M. Mastel, Thomas W. McMullin, Gregory M. Poynter, Brian J. Rush, Kevin T. Watts, Arlene M. Fosmer
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Publication number: 20130302866Abstract: The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.Type: ApplicationFiled: January 25, 2012Publication date: November 14, 2013Applicants: Bioamber S.A.S., Cargill, IncorporatedInventors: Kenneth R. Finley, Jeanette M. Huryta, Beth M. Mastel, Thomas W. McMullin, Gregory M. Poynter, Brian J. Rush, Kevin T. Watts, Arlene M. Fosmer, Vernon L. McIntosh, JR., Keith M. Brady
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Publication number: 20130302865Abstract: The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.Type: ApplicationFiled: July 23, 2013Publication date: November 14, 2013Applicant: NOVOZYMES, INC.Inventors: Amanda Fischer, Debbie Yaver
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Publication number: 20130295624Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: July 3, 2013Publication date: November 7, 2013Inventors: Marshall Medoff, Thomas Craig Masterman
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Patent number: 8574875Abstract: The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.Type: GrantFiled: August 14, 2008Date of Patent: November 5, 2013Assignee: BASF SEInventors: Edzard Scholten, Dirk Dägele, Stephan Haefner, Hartwig Schröder
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Publication number: 20130288322Abstract: A method for degrading a readily degradable resin composition comprising an aliphatic polyester (A) which is biodegradable, and an aliphatic polyester (B?) which releases an acid upon hydrolysis and which is biodegradable at a higher degradation rate than that of the aliphatic polyester (A), the method comprising degrading the readily degradable resin composition in an enzyme reaction liquid containing a degradation enzyme, and an acid neutralizing agent incompatible with the enzyme reaction liquid.Type: ApplicationFiled: June 27, 2013Publication date: October 31, 2013Applicant: TOYO SEIKAN KAISHA, LTD.Inventors: Seishi Yoshikawa, Tsutaki Katayama, Masahito Kogure
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Publication number: 20130288321Abstract: The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides.Type: ApplicationFiled: June 25, 2013Publication date: October 31, 2013Inventors: Sheryl Luttringer, Debbie Yaver
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Publication number: 20130288300Abstract: Provided are isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: February 23, 2012Publication date: October 31, 2013Applicant: Novoozymes A/SInventors: Yu Zhang, Junxin Duan, Lan Tang, Wenping Wu
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Publication number: 20130288319Abstract: The present invention provides tools and methods for producing organic acids using strains of Monascus which are tolerant to high organic acid concentrations at low pH.Type: ApplicationFiled: October 28, 2011Publication date: October 31, 2013Applicant: TOTAL RAFFINAGE MARKETINGInventors: Alexander Ruud Weusthuis, Emil Johan Harald Wolbert, Jan Springer, Jan Van Der Oost, Gerrit Eggink
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Publication number: 20130280766Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.Type: ApplicationFiled: October 31, 2011Publication date: October 24, 2013Applicant: The Regents of the Unversity of CaliforniaInventors: Jeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
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Publication number: 20130273601Abstract: The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.Type: ApplicationFiled: October 11, 2011Publication date: October 17, 2013Applicant: DSM IP ASSETS B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Publication number: 20130273608Abstract: A method of producing a compound originating from a polysaccharide-based biomass includes at least one of a saccharification step that produces a sugar solution containing a monosaccharide and/or an oligosaccharide from a product obtainable by hydrolyzing the polysaccharide-based biomass; a fermentation step that ferments the sugar solution containing the monosaccharide and/or oligosaccharide originating from the polysaccharide-based biomass; and a treatment that removes a fermentation inhibitor with the use of a separation membrane having a glucose removal rate and an isopropyl alcohol removal rate which simultaneously satisfy the following relationships (I) and (II) when a 500 ppm aqueous glucose solution at pH 6.5 at 25° C. and a 500 ppm aqueous isopropyl alcohol solution at pH 6.5 at 25° C. are respectively permeated through the membrane at an operation pressure of 0.Type: ApplicationFiled: June 12, 2013Publication date: October 17, 2013Inventors: Masayuki Hanakawa, Shinichi Minegishi, Hiroyuki Kurihara
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Publication number: 20130273613Abstract: The present disclosure identifies pathways and mechanisms to confer improved industrial fitness on engineered organisms. It also discloses engineered organisms having improved industrial fitness. Synthetic biologic engineering modules are disclosed that provide for light capture, carbon dioxide fixation, NADH production, NADPH production, thermotolerance, pH tolerance, flue gas tolerance, salt tolerance, nutrient independence and near infrared absorbance. The disclosed engineered organisms can include one or more of these modules. Also provided are methods of using the engineered organism to produce carbon-based products of interest, biomass or pharmaceutical agents.Type: ApplicationFiled: March 12, 2013Publication date: October 17, 2013Inventors: Eric James Devroe, Sriram Kosuri, David Arthur Berry, Noubar Boghos Afeyan, Frank Anthony Skraly, Dan Eric Robertson, Brian Green, Christian Perry Ridley
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Publication number: 20130266998Abstract: Described herein are compositions and methods for generating oxidoreductases for enantioselective reactions. Described herein are compositions and methods for generating neomorphic (R)-2-hydroxyacid dehydrogenases capable of enzymatically converting a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction. Illustrative examples include (a) (R)-2-hydroxyadipate dehydrogenase and uses thereof for converting 2-oxoadipate to (R)-2-hydroxyadipate, or the reverse reaction; and (b) (R)-2-hydroxyglutarate dehydrogenase and uses thereof for converting 2-oxoglutarate to (R)-2-hydroxyglutarate, or the reverse reaction. Also described herein are compositions and methods for generating non-natural microbial organisms to enzymatically convert 2-oxoadipate to (E)-2-hexenedioate or adipate, or to enzymatically convert 2-oxoglutarate to (E)-2-pentenedioate or glutarate, or the respective reverse reactions.Type: ApplicationFiled: February 27, 2013Publication date: October 10, 2013Inventors: Zachary James Reitman, Hai Yan, Bryan Daehahn Choi, John Howard Sampson
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Publication number: 20130269061Abstract: Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.Type: ApplicationFiled: March 11, 2013Publication date: October 10, 2013Inventors: Philip A. Lessard, Michael Lanahan, Vladimir Samoylov, Oleg Bougri, Jonas Emery, R. Michael Raab, Dongcheng Zhang
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Publication number: 20130260423Abstract: The present invention relates to methods of saccharifying the trash (leaf) fraction of sugar cane using enzymes.Type: ApplicationFiled: October 26, 2011Publication date: October 3, 2013Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Benjamin Knudsen, Armindo Ribeiro Gaspar
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Publication number: 20130260420Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: October 3, 2013Inventors: Marc D. Morant, Paul Harris
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Publication number: 20130252294Abstract: Disclosed herein are methods for producing compounds (such as 3,4-dihydroxybenzoate, catechol, cis,cis-muconate, or ?-carboxy-cis,cis-muconic acid) utilizing biosynthetic pathways in prokaryotic organisms expressing one or more heterologous genes. In some embodiments, the method includes expressing a heterologous asbF gene (for example, a gene having dehydroshikimate dehydratase activity) in a prokaryotic cell under conditions sufficient to produce the one or more compounds and purifying the compound. In additional embodiments, the method further includes expressing one or more of a heterologous 3,4-DHB decarboxylase gene, a heterologous catechol 1,2-dioxygenase gene, and a heterologous 3,4-DHB dioxygenase gene in the prokaryotic cell and purifying the compound.Type: ApplicationFiled: June 3, 2013Publication date: September 26, 2013Applicants: National University of Singapore, Los Alamos National Laboratory, LC/IPInventors: Andrew Thomas Koppisch, David Thomas Shaw Fox, Kinya Hotta, John D. Welsh
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Publication number: 20130225714Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: April 9, 2013Publication date: August 29, 2013Applicant: XYLECO, INC.Inventor: Xyleco, Inc.
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Publication number: 20130227748Abstract: The present invention relates to polypeptide having cellulolytic enhancing activity variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 29, 2011Publication date: August 29, 2013Applicant: Novozymes, Inc.Inventors: Matt Sweeney, Mark Wogulis