Enzyme Or Microbial Cell Is Immobilized On Or In An Organic Carrier Patents (Class 435/177)
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Patent number: 8574880Abstract: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, ?-hydroxylysine, and ?-hydroxy-?-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.Type: GrantFiled: February 27, 2009Date of Patent: November 5, 2013Assignee: Danisco US, Inc.Inventors: Risha Bond, Michael C. Jewett, Joseph C. McAuliffe, Donald E. Ward, II
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Publication number: 20130280781Abstract: The present invention provides a series of new and improved compounds/materials as vehicles to delivery degradative enzymes to remove/remedy environmental pollutants. The inventive material comprises a series of amide-functionalized ordered mesoporous carbon (AFOMC), which utilizes chemical conjugation techniques for the tethering of enzymes to the surface of the synthesized AFOMC. The delivery mechanism may be utilized to express a wide variety of toxin-degrading enzymes for removal/remediation of organic pollutants.Type: ApplicationFiled: April 16, 2012Publication date: October 24, 2013Applicant: The Curators of the University of MissouriInventors: Chung-Ho Lin, Brian Thompson
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Publication number: 20130259919Abstract: A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels.Type: ApplicationFiled: May 24, 2013Publication date: October 3, 2013Inventor: Steven T. Boyce
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Patent number: 8546493Abstract: Multi-armed, monofunctional, and hydrolytically stable polymers are described having the structure wherein Z is a moiety that can be activated for attachment to biologically active molecules such as proteins and wherein P and Q represent linkage fragments that join polymer arms polya and polyb, respectively, to central carbon atom, C, by hydrolytically stable linkages in the absence of aromatic rings in the linkage fragments. R typically is hydrogen or methyl, but can be a linkage fragment that includes another polymer arm. A specific example is an mPEG disubstituted lysine having the structure where mPEGa and mPEGb have the structure CH3O—(CH2CH2O)nCH2CH2— where n may be the same or different for polya- and polyb- and can be from 1 to about 1,150 to provide molecular weights of from about 100 to 100,000.Type: GrantFiled: December 14, 2012Date of Patent: October 1, 2013Assignee: Nektar TherapeuticsInventors: J. Milton Harris, Francesco M. Veronese, Paolo Caliceti, Oddone Schiavon
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Publication number: 20130251637Abstract: Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.Type: ApplicationFiled: December 20, 2011Publication date: September 26, 2013Applicants: DYOMICS GMBH, PIERCE BIOTECHNOLOGY, INC.Inventors: Greg Hermanson, Peter T. Czerney, Surbhi Desai, Matthias S. Wenzel, Boguslawa Dworecki, Frank G. Lehmann
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Patent number: 8541625Abstract: Cyclic alkynes (e.g., cyclooctynes such as dibenzocyclooctynes) can be photochemically generated from cyclopropenones as disclosed herein. The cyclic alkynes can be reacted (e.g., in situ) with materials having alkyne-reactive groups (e.g., azide groups in a “click” reaction). In preferred embodiments, the generation and reaction of the cyclic alkyne can proceed in the absence of a catalyst (e.g., Cu(I)). These reactions can be useful, for example, for the selective labeling of living cells that are metabolically modified with azido-containing surface monosaccharides, or for light-directed surface patterning.Type: GrantFiled: August 6, 2012Date of Patent: September 24, 2013Assignee: University of Georgia Research Foundation, Inc.Inventors: Vladimir V. Popik, Andrei A. Poloukhtine, Geert-Jan Boons, Margaretha Wolfert
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Patent number: 8535924Abstract: A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.Type: GrantFiled: November 3, 2011Date of Patent: September 17, 2013Assignee: Danisco US Inc.Inventors: Douglas A. Dale, Thomas S. Green
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Publication number: 20130224799Abstract: A method is disclosed for improved isothermal amplification of nucleic acids comprising the step of release of an essential component from a matrix under predetermined conditions. Furthermore, the invention relates to a kit comprising mesophilic enzyme and a matrix with embedded essential components for isothermal amplification. A composition comprising a matrix and a mesophilic enzyme and a method for embedding a mesophilic enzyme are disclosed as well.Type: ApplicationFiled: August 9, 2011Publication date: August 29, 2013Applicant: QIAGEN GMBHInventor: Christian Korfhage
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Publication number: 20130224824Abstract: An objective of the present invention is to establish a technique for making it possible to immobilize an enzyme on an electrically conductive base material in a uniformly, high density, and constantly aligned orientation, for the purpose of constructing an enzyme electrode having improved electrode performance. An electrode having enzyme crystals immobilized thereon, the electrode being provided with an electrically conductive base material that can be connected to an external circuit and enzyme crystals that serve as an electrode catalyst, wherein the enzyme crystals are immobilized on the electrically conducive base material; a method for producing an electrode having enzyme crystals immobilized thereon; and a biological fuel cell and a biosensor which are provided with an electrode having enzyme crystals immobilized thereon.Type: ApplicationFiled: August 24, 2011Publication date: August 29, 2013Applicants: Riken, Aisin Seiki Kabushiki KaishaInventors: Yasushi Shigemori, Yuichiro Nakaoki, Tsutomu Mikawa
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Patent number: 8518682Abstract: A tissue engineering composition adapted for application to an interior surface of a body lumen of a patient. The composition comprises (a) a carrier medium that is adapted to flow and to stably adhere the composition to the body lumen and (b) microcapsules, which are dispersed within the carrier medium and which contain one or more living cells encapsulated within a coating that includes a biodegradable polymer. The composition promotes growth of the cells on the lumen surface subsequent to application of the composition to the lumen.Type: GrantFiled: December 13, 2010Date of Patent: August 27, 2013Assignee: Boston Scientific Scimed, Inc.Inventors: Toby M. Freyman, Joseph A. Levendusky
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Patent number: 8518683Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: GrantFiled: July 20, 2012Date of Patent: August 27, 2013Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20130203146Abstract: The present invention relates to a method for producing a three-dimensional scaffold construct comprising encapsulated cells, the method comprising: (a) providing a solution comprising cells, a photoinitiator, and a plurality of units capable of forming polymer chains; (b) providing a photolithography instrument comprising a two-photon laser; and (c) using the instrument to apply the laser to the solution to activate the photoinitiator thereby facilitating polymerisation of said units to form polymer chains, and, cross-linking of the polymer chains; wherein the laser is applied to the solution in three-dimensions in a pre-defined pattern to assemble said construct, and said cells are encapsulated within the assembled construct.Type: ApplicationFiled: August 3, 2011Publication date: August 8, 2013Inventors: Jackie Y. Ying, Tseng-Ming Hsieh, Andrew Chwee Aun Wan
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Publication number: 20130202564Abstract: This invention provides a composition for controlled-release of polypeptide growth factors useful in tissue repair or engineering. The composition include a polypeptide growth factor covalently cross-linked to a biocompatible substrate by a transgultaminase. The cross-linking tethers the growth factor to a substrate so that it will not diffuse away from the desired site of application. It also concomitantly inactivates the growth factor. Release and reactivation of the growth factor can be achieved by endogenously produced metalloproteinase (MMPs) or exogenously provided proteases such as collagenases.Type: ApplicationFiled: April 8, 2011Publication date: August 8, 2013Applicant: UNIVERSITY OF SOUTHERN CALIFORNIAInventors: Bo Han, Kenrick Kuwahara
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Patent number: 8497088Abstract: The present invention describes a process for the synthesis of a semi-synthetic ?-lactam compound from a nucleus and a side chain selected from the group consisting of D-phenylglycine and D-dihydro-phenylglycine in the form of a side chain ester and an enzyme catalyzing the coupling of the side chain ester to the nucleus characterized in that the side chain ester is not isolated as a solid intermediate.Type: GrantFiled: March 10, 2008Date of Patent: July 30, 2013Assignee: DSM Sinochem Pharmaceuticals Netherlands B.V.Inventors: Thomas Van Der Does, Harold Monro Moody, Theodorus Johannes Godfried Maria Van Dooren
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Patent number: 8497107Abstract: A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.Type: GrantFiled: September 2, 2009Date of Patent: July 30, 2013Assignee: Fresenius Medical Care Holdings, Inc.Inventor: Stephen A. Merchant
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Patent number: 8492129Abstract: A method of reacting a first chemical entity and a second chemical entity to form a conjugate in which the first and second chemical entities are covalently bound with respect to each other, comprises bringing into simultaneous contact the first chemical entity, the second chemical entity and a thiol generator, wherein the thiol generator reacts with the first chemical entity in a thiolation reaction resulting in formation of a free sulfhydryl group on the first chemical entity, and the free sulfhydryl group reacts with the second chemical entity to form the conjugate, and wherein the second chemical entity is polyvalent with respect to its reactivity with sulfhydryl groups. The present invention primarily differs from the prior art in that no separation step is involved between reaction of the thiol generator and first chemical entity and reaction with the second chemical entity. The invention also provides a conjugation kit.Type: GrantFiled: December 12, 2006Date of Patent: July 23, 2013Assignee: Innova Biosciences, Ltd.Inventors: Nicholas Gee, Michael Knowles
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Publication number: 20130180915Abstract: The present invention is directed to a composition for triggering microbiological processes in water, to a kit of parts comprising the components of said composition in distinct form, a method of producing such a composition and the use thereof for microbiologically decomposing water-immiscible substances, in particular crude oil and crude oil-derived substances.Type: ApplicationFiled: July 14, 2011Publication date: July 18, 2013Applicant: Lavaris Technologies GmbHInventor: Thomas Willuweit
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Publication number: 20130183734Abstract: A phospholipase-phospholipase-carrier complex for degumming crude oils. The phospholipase-carrier complex includes at least one phospholipase A1, A2 or B and at least one carrier, such as silicic acid, precipitated silicic acid, polyacrylate, polymethacrylate or divinylbenzene-crosslinked methacrylate. The disclosure further relates to a method of degumming crude oils and to a method of producing the phospholipase-carrier complex.Type: ApplicationFiled: December 28, 2012Publication date: July 18, 2013Applicant: CLARIANT PRODUKTE (DEUTSCHLAND) GMBHInventor: Clariant Produkte (Deutschland) GmbH
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Publication number: 20130172705Abstract: The present invention is broadly concerned with the crafting and manufacturability of an implantable enzymatic-based sensor characterized by a small size, optimum geometry, linearity of response over the concentration range of interest, extended shelf-life, selectivity for the analyte in question, and the ability to exclude bioactive interferents. More particularly, it is preferably concerned with a general approach to optimize the performance of the biorecognition elements required to produce biosensors of the type designed to provide, and in conjunction with a suitable signal processing unit, a current which is proportional to the concentration of the analyte of interest. The biosensors described herein may be implanted in vivo, including intra-cerebral, sub-cutaneous, intra-muscular, inter-peritoneal oral, serum, and vascular implantation, the majority of which may act as a surrogate for systemic monitoring and used to monitor analytes of interest in real-time.Type: ApplicationFiled: September 12, 2011Publication date: July 4, 2013Applicant: PINNACLE TECHNOLOGY, INC.Inventors: Peter A. Petillo, Daniel V. Aillon, Brian S. Barrett
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Publication number: 20130172985Abstract: Described herein are modified gelatins or the pharmaceutically-acceptable salts or esters thereof comprising at least one actinically crosslinkable group covalently bonded to gelatin. The modified gelatins are useful in producing composites that ultimately can be used to produce three-dimensional engineered biological constructs. The composites are the polymerization product between the modified gelatin and at least one actinically crosslinkable macromolecule. Methods for making the modified gelatins are also described herein.Type: ApplicationFiled: January 13, 2011Publication date: July 4, 2013Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATIONInventors: Glenn D. Prestwich, Aleksander Skardal, Jianxing Zhang
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Publication number: 20130171712Abstract: Provided are a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. A culture method of culturing cells adhered onto an electrode layer, the method including: a first step of adhering the cells onto the electrode layer and culturing the cells thereon, the electrode layer being coated with an oligopeptide including: a terminal amino acid; a cell adhesive sequence; and an alternating sequence to be bound to the one end side of the cell-adhesive sequence, the alternating sequence including a plurality of acidic amino acids and a plurality of basic amino acids, being alternately bound to each other one by one; and a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer.Type: ApplicationFiled: September 8, 2011Publication date: July 4, 2013Applicant: UNIVERSITY OF TSUKUBAInventors: Junji Fukuda, Hiroaki Suzuki, Naoto Mochizuki, Takahiro Kakegawa
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Publication number: 20130173018Abstract: A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.Type: ApplicationFiled: September 14, 2011Publication date: July 4, 2013Applicant: TOKYO WOMEN'S MEDICAL UNIVERSITYInventors: Katsuhisa Sakaguchi, Tatsuya Shimizu, Hidekazu Sekine, Mitsuo Umezu, Teruo Okano
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Publication number: 20130164803Abstract: Cyclic alkynes (e.g., cyclooctynes such as dibenzocyclooctynes) can be photochemically generated from cyclopropenones as disclosed herein. The cyclic alkynes can be reacted (e.g., in situ) with materials having alkyne-reactive groups (e.g., azide groups in a “click” reaction). In preferred embodiments, the generation and reaction of the cyclic alkyne can proceed in the absence of a catalyst (e.g., Cu(I)). These reactions can be useful, for example, for the selective labeling of living cells that are metabolically modified with azido-containing surface monosaccharides, or for light-directed surface patterning.Type: ApplicationFiled: August 6, 2012Publication date: June 27, 2013Applicant: University of George Research Foundation, Inc.Inventors: VLADIMIR V. POPIK, Andrei A. Poloukhtine, Geert-Jan Boons, Margaretha Wolfert
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Patent number: 8460907Abstract: The present invention provides a thermoresponsive nanoparticle useful for the stabilization of enzymes in environments having a temperature greater than thirty degrees Centigrade. The thermoresponsive nanoparticle has (a) a functionalized enzyme conjugate having one or more enzymes or biological catalysts, the enzymes or biological catalysts are modified with palmitic acid N-hydroxysuccinimide ester and acrylic acid N-hydroxysuccinimide ester, and (b) a thermally responsive polymer, wherein the functionalized enzyme conjugate is encapsulated within the thermally responsive polymer. A nanocatalyst is provided that has one or more proteins. The proteins are covalently immobilized and encapsulated within a thermally responsive polymer shell. The proteins are one or more enzymes or biological catalysts. A method for protecting the proteins is also set forth.Type: GrantFiled: November 11, 2010Date of Patent: June 11, 2013Assignee: ICx-AgentaseInventors: Jeremy P. Walker, Anna M. Leech
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Patent number: 8460546Abstract: The present invention relates to bioartificial devices and systems that mimic kidney or nephron function and methods of making them.Type: GrantFiled: June 16, 2011Date of Patent: June 11, 2013Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Eli Weinberg, Jeffrey T. Borenstein
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Patent number: 8460269Abstract: The disclosed technology describes compositions and methods useful for providing cell based therapy. For example, one embodiment of cell based therapy involves the regeneration of injured tissue and/or promoting wound healing. Certain embodiments provide improved therapeutic compositions using microbubbles by delivering biological progenitor cells to the injured tissues. The administration of the microbubbles is directed by acoustic radiation forces that interact with embodiments of microbubbles comprising an acoustically active gas. As such, a high efficiency of progenitor cell delivery to injured tissue is realized. One advantage of this technique over targeted delivery of pharmaceutical compounds, is that the delivered progenitors cells may be derived from the patient (i.e., personalized therapy), thereby avoiding side effects, allergic reactions, and overall problems associated with refractive drug responses.Type: GrantFiled: September 13, 2010Date of Patent: June 11, 2013Assignee: University of Pittsburgh—Of the Commonwealth System of Higher EducationInventors: Catalin Toma, Flordeliza Villanueva, William Richard Wagner, Joon S. Lee, Jianjun Wang, Xucai Chen, Andrew Fisher
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Patent number: 8450081Abstract: Identification of infectious pathogens, particularly viruses, bacteria and other microorganisms is effected with a method whereby pathogens of acute infections can be identified, without first culturing them in external nutrient media, by mass spectrometric measurement of their protein profiles obtained from pathogens directly precipitated from body fluid into pellets by centrifuging. With this method, pathogens which cause acute infections can be identified in less than one hour.Type: GrantFiled: November 20, 2008Date of Patent: May 28, 2013Assignee: Bruker Daltonik GmbHInventor: Ulrich Weller
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Publication number: 20130131701Abstract: Patches comprising dry gelatin compositions and methods of use and manufacture thereof.Type: ApplicationFiled: August 5, 2011Publication date: May 23, 2013Applicant: LIFEBOND LTD.Inventors: Chagai Komlos, Guy Tomer, Maria Ziv, Orahn Preiss-Bloom, Thomas Bayer
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Publication number: 20130130284Abstract: Disclosed is a composite of enzyme and fiber matrix with three-dimensional structure. The composite of enzyme and fiber matrix with three-dimensional structure includes a significantly large amount of an enzyme loaded in and immobilized in/onto a matrix when compared to conventional composites. In addition, the immobilized enzyme is prevented from leaching from the matrix when an external impact is applied to the composite of enzyme and fiber matrix with three-dimensional structure. Therefore, the stability of the composite of enzyme and fiber matrix with three-dimensional structure of the present invention is maintained even after a long period passes since a remarkably great amount of enzymes compared with a known composite can be supported and immobilized to a matrix, and the immobilized enzyme is not easily released by an external impact.Type: ApplicationFiled: April 19, 2011Publication date: May 23, 2013Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATIONInventors: Jungbae Kim, Hyeong-Seok Kim
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Publication number: 20130122492Abstract: The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex. The complex can be detected or isolated and subsequently analyzed by any of a variety of physical, chemical and genetic techniques.Type: ApplicationFiled: November 14, 2011Publication date: May 16, 2013Applicant: KELLBENX INC.Inventors: Javad Khosravi, Leonard H. Kellner, Hassan Benanni
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Patent number: 8440441Abstract: A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain comprising: a moiety R that is chemically coupled to the substrate, said moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker comprising at least one nucleophilic group. Methods of preparing the substrate and use of the substrate are also disclosed.Type: GrantFiled: February 18, 2011Date of Patent: May 14, 2013Assignee: Temasek PolytechnicInventors: Christian Bluchel, Yanmei Wang
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Publication number: 20130115590Abstract: The present disclosure relates to immobilization of a cell using a carboxylated surface by contacting the carboxylated surface with a sample comprising the cell for a sufficient time to permit the cell to bind to the carboxylated surface. The immobilized cell may then be separated from the remainder of the sample and further manipulated to isolate, concentrate, and/or analyze the cell or a component thereof.Type: ApplicationFiled: November 2, 2012Publication date: May 9, 2013Applicant: QIAGEN GAITHERSBURG, INC.Inventor: Qiagen Gaithersburg, Inc.
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Publication number: 20130109072Abstract: A support for enzyme immobilization is described, which is for immobilizing enzymes of various molecular sizes and also for, due to the modification of the surface silanol groups of porous silica particles, for immobilizing various kinds of enzymes, and enables the design of an immobilized enzyme, which exhibits an activity equivalent to that of the corresponding non-immobilized enzyme and withstands repeated use. A method for producing the support is also described. The support includes porous silica particles having an interparticle void structure therein, characterized in that the porous silica particles have a specific average particle size, a specific surface area, a specific pore volume, a specific pore size distribution and a specific porosity and have a substituent containing an organic group or an amino group on the surface thereof. An immobilized protein obtained by immobilizing a protein on the above support is also described.Type: ApplicationFiled: June 8, 2011Publication date: May 2, 2013Applicants: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, JGC CORPORATIONInventors: Tatsuo Tsunoda, Takayuki Nara, Seigo Ono, Chisato Sekikawa, Fujio Mizukami, Shuzo Kojima, Naoki Tahara, Hideaki Togashi, Miki Egami
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Patent number: 8431146Abstract: A device for inhibiting adhesion of apposing human body tissue layers includes a scaffold having a designated mean pore size, relative density, and degradation half-life. The scaffold may be operably positioned between apposing tissue layers, such as proximate adhesiogenic layers at a wound site, so as to permit remesothelialization of the tissue without formation of fibrous adhesions. The scaffold device of the invention inhibits adhesion formation by promoting contractile cell migration away from the wound site for a predetermined period of time. The invention further relates to device and methods for promoting internal tissue regeneration, and for provision and/or dispensation of therapeutic and/or diagnostic agents in vivo.Type: GrantFiled: March 17, 2010Date of Patent: April 30, 2013Assignee: Axle International Ltd.Inventors: Brendan A. Harley, Eric C. Soller, Eric Aiazian
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Publication number: 20130102048Abstract: A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.Type: ApplicationFiled: October 12, 2012Publication date: April 25, 2013Applicant: TAKARA BIO INC.Inventor: TAKARA BIO INC.
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Patent number: 8426192Abstract: An electrochemical sensor system and membrane and method thereof for increased accuracy and effective life of electrochemical and enzyme sensors.Type: GrantFiled: December 14, 2009Date of Patent: April 23, 2013Assignee: Instrumentation Laboratory CompanyInventors: Prasad Pamidi, Sohrab Mansouri, Melanie Shin, Vasile Cosofret, Clarke Xu
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Patent number: 8415124Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.Type: GrantFiled: July 20, 2010Date of Patent: April 9, 2013Assignee: Kao CorporationInventors: Manabu Sato, Kazuhiro Onozuka, Takaaki Watanabe, Hiroyuki Konishi
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Patent number: 8409447Abstract: A separation chip for separating an insoluble component from a suspension using centrifugal force by rotation, includes a suspension holding tank, a separation liquid holding tank, and an insoluble component holding tank arranged in order from an inner circumferential side during rotation, wherein the suspension holding tank and the insoluble component holding tank are connected to each other, the insoluble component holding tank and the separation liquid holding tank are connected to each other by a narrow portion, and in the insoluble component holding tank, a connecting portion with the suspension holding tank is positioned further toward an outer circumferential side than the narrow portion.Type: GrantFiled: January 27, 2009Date of Patent: April 2, 2013Assignee: Toray Industries, Inc.Inventors: Kentaro Ishii, Masashi Higasa, Shingo Hiramatsu
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Patent number: 8404470Abstract: The invention is directed to enzyme preparations which are obtainable by enzyme immobilizates which comprise enzymes or microorganisms containing enzymes immobilized on an inert carrier being provided with a silicone coating obtained by hydrosilylation, to a process for producing such enzyme preparations and to the use of enzyme preparations as an industrial biocatalyst.Type: GrantFiled: July 7, 2008Date of Patent: March 26, 2013Assignee: Evonik Goldschmidt GmbHInventors: Oliver Thum, Marion Ansorge-Schumacher, Lars Wiemann, Andreas Buthe
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Patent number: 8394350Abstract: A method for separating CO2 from gas mixtures uses a slurried media impregnated with buffer compounds and coating the solid media with a catalyst or enzyme that promotes the transformation of CO2 to carbonic acid. Buffer sorbent pebbles with a catalyst or enzyme coating are provided for rapid separation of CO2 from gas mixtures.Type: GrantFiled: May 19, 2010Date of Patent: March 12, 2013Assignee: Lawrence Livermore National Security, LLCInventor: Roger D. Aines
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Patent number: 8388989Abstract: Articles of clothing treated with bacteria adapted to metabolize a component selected from the group consisting of ammonia, ammonium salts, or urea into any of nitric oxide, nitric oxide precursors, and combinations thereof are provided.Type: GrantFiled: September 24, 2010Date of Patent: March 5, 2013Assignee: Nitrocell BioSciences LLCInventor: David R. Whitlock
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Publication number: 20130048559Abstract: A substrate having compounds disposed thereon for immobilizing a functional molecule, each compound having a chain comprising: a moiety R that is chemically coupled to the substrate, said moiety R being selected from the group consisting of an ether, ester, carbonyl, carbonate ester, thioether, disulfide, sulfinyl, sulfonyl, and carbonothioyl; and an epoxide-containing moiety that is coupled to the moiety R by a linker comprising at least one nucleophilic group. Methods of preparing the substrate and use of the substrate are also disclosed.Type: ApplicationFiled: February 18, 2011Publication date: February 28, 2013Applicant: TEMASEK POLYTECHNICInventors: Christian Bluchel, Yanmei Wang
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Publication number: 20130052179Abstract: The present invention relates to an arginine deiminase mutant with partial lysine-deficient and preparation and application thereof. The arginine deiminase mutant of the present invention has enzymatic activity of degrading arginine into citruline; compared with the arginine deiminase with the amino acid sequence of SEQ ID NO: 1, the amino acid sequence comprises one or more of K9N, T, K59Q, K66R, A, K93E, A, Q, K111R, A, K119Q, L, M, K121Q, I, K122E, L, K126E, S, R, K178I, E, D, K196I, R, K209G, T, D, K243E, V, R, K249D, Q, K263N, Q, K279Y, T, K293R, H, E, K325V, I, K380T, R, E, and K406E, D, S substitutions. Compared with PEG modified natural derived arginine deiminase, the PEG modified arginine deiminase mutant of the present invention retain better bioactivity; and because the quantity of lysine in arginine deiminase is reduced, the PEG modified products are more uniform and can be applied to clinical treatment of hepatoma, melanoma and the like.Type: ApplicationFiled: August 22, 2011Publication date: February 28, 2013Inventors: Yanshan HUANG, Jiwan Qiu, Xiaoyu Fu, Min Fan, Yujiao Wang, Yefei Wang
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Publication number: 20130052712Abstract: The present invention relates to technology of immobilizing or coating various functional bioactive substances on various surfaces without physical chemical treatment using mussel adhesive protein. More specifically, the present invention relates to a functional scaffold for tissue engineering comprising artificial extracellular matrix, manufactured by coating various functional bioactive substances on the surface of nanofiber and metal scaffold using mussel adhesive protein, and a method of manufacturing the same.Type: ApplicationFiled: March 26, 2012Publication date: February 28, 2013Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventors: Hyung Joon Cha, Bum Jin Kim, Yoo Seong Choi, Bong-Hyuk Choi
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Patent number: 8383782Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 30, 2009Date of Patent: February 26, 2013Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Patent number: 8383378Abstract: Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micro-mechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.Type: GrantFiled: October 9, 2006Date of Patent: February 26, 2013Assignee: The Regents of the University of CaliforniaInventors: Yuli Wang, Mark Bachman, Christopher E. Sims, Guann-Pyng Li, Nancy Allbritton
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Publication number: 20130045521Abstract: A support apparatus for maintaining, storing, and transporting biological materials, such as cells. In accordance with certain embodiments of the invention, the biologic materials may be integrated, to an electronic component to form a biological chip device, which may be supported, transported, and adapted for interconnection with other such, devices through, the use of a support apparatus in accordance with embodiments of the invention. In certain embodiments, the support apparatus can generate signals and/or translate signals received for processing and/or relaying to another device or module (e.g., another support apparatus). A plurality of biological chip devices and associated support apparatuses can be supported and linked in a network to perform various desired functions.Type: ApplicationFiled: October 23, 2012Publication date: February 21, 2013Inventor: Spencer Z Rosero
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Publication number: 20130045244Abstract: Modified ADAM (A Disintegrin and Metalloproteinase) Polypeptides (MAPs) are provided. Methods are provided for administering MAPs for anti-angiogenesis and anti-tumor growth activity. Compositions of the invention are also useful for treating endothelial cell dysfunction and for diagnosis of integrin-related conditions.Type: ApplicationFiled: February 9, 2011Publication date: February 21, 2013Inventors: Radu O. Minea, Stephen D. Swensen, Francis S. Markland, JR.
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Patent number: 8377668Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed for use in the production of useful products, such as fuels. For example, systems can use biomass materials, such as cellulosic and/or lignocellulosic materials, to enhance the production of a product, e.g., the production of ethanol and/or butanol by fermentation.Type: GrantFiled: May 18, 2010Date of Patent: February 19, 2013Assignee: Xyleco, Inc.Inventors: Marshall Medoff, Thomas Craig Masterman, Harrison D. Medoff
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Publication number: 20130035257Abstract: Methods of isolating at least one cell of interest, methods of making fixed arrays, arrays comprising a glass substrate bonded to a patterned siloxane structure having inlets, outlets and microchannels, array kits, and methods of making microfluidic apparati are provided in the present application.Type: ApplicationFiled: September 10, 2012Publication date: February 7, 2013Applicant: UNIVERSITY OF BRITISH COLUMBIAInventors: Linfen YU, Karen C. CHEUNG