Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
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Patent number: 8581040Abstract: The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.Type: GrantFiled: April 27, 2011Date of Patent: November 12, 2013Assignee: Plant Sensory Systems, LLCInventors: Frank J. Turano, Kathleen A. Turano
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Publication number: 20130295644Abstract: The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.Type: ApplicationFiled: October 28, 2011Publication date: November 7, 2013Inventors: Bjarke Christensen, Peter Bjarke Olsen, Torsten Bak Regueira, Steen Troels Joergensen, Alan Berry
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Patent number: 8577622Abstract: The present invention relates to processes for the screening, preparation and characterization of (R)-selective ?-transaminases, to transaminases obtained thereby and their uses in various transamination processes.Type: GrantFiled: August 13, 2010Date of Patent: November 5, 2013Assignee: Lonza AGInventors: Matthias Hoehne, Uwe Bornscheuer, Karen Robins, Sebastian Schaetzle
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Publication number: 20130288377Abstract: Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms.Type: ApplicationFiled: April 26, 2013Publication date: October 31, 2013Applicant: Synthetic Genomics, Inc.Inventors: Michele M. Champagne, Jun Urano
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Patent number: 8569021Abstract: The subject invention provides novel microorganisms comprising polynucleotides and polypeptides encoding a methyltransferase. The subject invention provides novel methods for the production of biodiesel.Type: GrantFiled: December 14, 2010Date of Patent: October 29, 2013Assignee: University of Tennessee Research FoundationInventors: Feng Chen, Nan Zhao
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Publication number: 20130280258Abstract: Disclosed herein are methods and compositions for the treatment of cancer. In particular, the present invention identifies and characterizes the FANCI polypeptide as a vital component of the Fanconi anemia pathway and discloses inhibitors of FANCI and methods of using same. Such inhibitors are useful in inhibiting DNA damage repair and can be useful, for example, in the treatment of cancer.Type: ApplicationFiled: March 6, 2012Publication date: October 24, 2013Applicants: Miltenyi Biotec GmbH, The Brigham & Women's Hospital, Inc.Inventors: Alan D. D'Andrea, Patrizia Vinciguerra, Stephen J. Elledge, Shuhei Matsuoka, Agata M. Smogorzewska, Kay O. Hofmann
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Publication number: 20130283484Abstract: The present invention relates to a gene encoding SyGT (Synechocystis glucosyl transferase) protein derived from cyanobacteria (Synechocystis) PCC 6906, a method for enhancing salt tolerance of a plant comprising transforming a plant cell with a recombinant vector comprising the SyGT gene and overexpressing the SyGT gene, a plant having enhanced salt tolerance produced by the method, and seed of the plant.Type: ApplicationFiled: October 13, 2011Publication date: October 24, 2013Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Jang Ryol Liu, Suk Weon Kim, Jong Hyun Kim, Sung Ran Min, Won Joong Jeong, Myung Suk Ahn, Young Min Park, Myung Jin Oh, Ji Hyun Park
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Publication number: 20130280785Abstract: The present invention relates to a producing and purifying method of soluble recombinant COQ5 protein, which is expressed in soluble form by E. coli, under native conditions. The method is characterized by pre-treating bacterial lysate with low concentration of ionic detergent, such as SDS, before purification; and the purifying method is performed under native condition without using urea to avoid the problems of requiring lengthy processes to remove urea in purified protein solution or re-aggregation and precipitation of protein after removal of urea.Type: ApplicationFiled: July 8, 2013Publication date: October 24, 2013Inventors: HSIU-CHUAN YEN, SHIH-WEI CHEN
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Publication number: 20130280763Abstract: The present invention relates to the use of Archease proteins as RNA ligase enhancer, methods of ligating RNA molecules, kits for these methods and uses and transgenic cells.Type: ApplicationFiled: April 23, 2013Publication date: October 24, 2013Applicant: IMBA - INSTITUT FUR MOLEKULARE BIOTECHNOLOGIE GMBHInventors: Johannes POPOW, Javier MARTINEZ, Anne NIELSEN, Alexander SCHLEIFFER
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Publication number: 20130280766Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.Type: ApplicationFiled: October 31, 2011Publication date: October 24, 2013Applicant: The Regents of the Unversity of CaliforniaInventors: Jeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
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Patent number: 8562977Abstract: Pharmaceutical compositions comprising truncated tryptophanyl-tRNA synthetase polypeptides useful for regulating angiogenesis an nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.Type: GrantFiled: March 30, 2012Date of Patent: October 22, 2013Assignee: The Scripps Research InstituteInventors: Paul Schimmel, Keisuke Wakasugi
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Patent number: 8563285Abstract: The present invention relates to unique isoforms of eukaryotic initiation Factor 5A (“eIF-5A”): senescence-induced eIF-5A; wounding-induced eIF-5A; and growth eIF-5A, as well as polynucleotides that encode these three factors. The present invention also relates to methods involving modulating the expression of these factors. The present invention also relates to deoxyhypusine synthase (“DHS”), polynucleotides that encode DHS, and methods involving modulating the expression of DHS.Type: GrantFiled: December 9, 2010Date of Patent: October 22, 2013Assignee: Senesco Technologies, Inc.Inventors: John E. Thompson, Tzann-Wei Wang, Dongen Lily Lu
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Patent number: 8563278Abstract: A process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin is described. This process entails converting a substituted ?-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same.Type: GrantFiled: August 10, 2010Date of Patent: October 22, 2013Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Nao Funakoshi, Yusuke Amino, Shigeru Kawahara, Tadashi Takemoto
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Patent number: 8563286Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: February 7, 2013Date of Patent: October 22, 2013Assignees: Novozymes, Inc., Novozymes A/SInventors: Debbie Yaver, Janine Lin, Michael Lamsa, Kim Borch, Shamkant Anant Patkar
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Publication number: 20130273615Abstract: The present invention relates to a polypeptide that is modified to have homoserine O-acetyltransferase activity, and in particular, the present invention provides a modified polypeptide having homoserine O-acetyltransferase activity, in which the amino acid at position 111 of a polypeptide having homoserine succinyltransferase activity is substituted with other amino acid.Type: ApplicationFiled: December 21, 2011Publication date: October 17, 2013Applicant: CJ CHEILJEDANG CORPORATIONInventors: So Young Kim, Yong Uk Shin, Chang Il Seo, In Kyung Heo, Ju Eun Kim, Hyun Ah Kim, Han Jin Lee, Kwang Ho Na, Sung Kwang Son
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Publication number: 20130273614Abstract: The present invention relates to a novel protein having O-acetylhomoserine sulfhydrylase activity, a mutant protein thereof, a polynucleotide encoding the same, a recombinant vector comprising the polynucleotide, a microorganism transformed with the recombinant vector, and a method for producing methionine or acetic acid using the protein. The production method of the present invention has the advantage of producing L-methionine and acetic acid cost-effectively through having higher conversion rate and reduced reaction time compared to the existing methods, and it can minimize the amount of enzyme homogenate added when using the mutant protein, thereby easily producing L-methionine and acetic acid at high yield.Type: ApplicationFiled: December 21, 2011Publication date: October 17, 2013Applicant: CJ CHEILJEDANG CORPORATIONInventors: So Young Kim, Yong Uk Shin, Chang Il Seo, Sung Kwang Son, In Kyung Heo, Han Jin Lee, Ju Eun Kim, Hyun Ah Kim, Jee Yeon Bae, Kwang Ho Na
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Publication number: 20130273601Abstract: The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.Type: ApplicationFiled: October 11, 2011Publication date: October 17, 2013Applicant: DSM IP ASSETS B.V.Inventors: Hendrik Wouter Wisselink, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk
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Publication number: 20130267696Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an ?-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the ?-olefin.Type: ApplicationFiled: September 28, 2011Publication date: October 10, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Jeffrey L. Fortman, Leonard Katz, Eric J. Steen, Jay D. Keasling
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Publication number: 20130266994Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.Type: ApplicationFiled: June 18, 2013Publication date: October 10, 2013Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
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Publication number: 20130260436Abstract: The invention relates to methods and compositions for identifying a candidate nucleic acid (CNA) comprising a polynucleotide sequence encoding at least a part of a natural product gene cluster (NPGC), a secondary metabolite biosynthesis cluster (SMBC), a non ribosomal peptide (NRP), a polyketide (PK) biosynthesis cluster, a protein involved in NRP and/or PK biosynthesis, a protein involved in other secondary metabolite biosynthesis, and/or a phosphopantetheinyl transferase (PPTase), by expressing the candidate nucleic acid (CNA) to form at least one PPTase, incubating the PPTase with a non ribosomal peptide synthetase (NRPS), and detecting activation of the NRPS, wherein activation indicates that said CNA comprises a polynucleotide sequence encoding at least one of the above.Type: ApplicationFiled: September 14, 2011Publication date: October 3, 2013Applicant: Victoria LInk LimitedInventors: Davis Francis Ackerley, Jeremy Owen
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Publication number: 20130260424Abstract: Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.Type: ApplicationFiled: March 15, 2013Publication date: October 3, 2013Applicant: CJ Cheiljedang CorporationInventors: Brian BRAZEAU, Jin-Sook Chang, Kwang Myung Cho, Young Wook Cho, Mervyn Desouza, Holly J. Jessen, So-Young Kim, Wei Niu, Fernando A. Sanchez-Reira, Yong-Uk Shin, Hyewon Um
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Patent number: 8546118Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: March 7, 2007Date of Patent: October 1, 2013Assignee: Verenium CorporationInventors: David P. Weiner, Lishan Zhao, Peter Luginbuhl, Ellen Burke, Toby Richardson, Paula M. Hicks
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Patent number: 8546105Abstract: Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.Type: GrantFiled: August 9, 2010Date of Patent: October 1, 2013Assignees: The University of Wyoming, The John Hopkins University, Human Genome Sciences, Inc.Inventors: Donald Jarvis, Michael J. Betenbaugh, Shawn Lawrence, Yuan C. Lee, Timothy A. Coleman
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Patent number: 8546111Abstract: The object is to provide a novel glycosyltransferase and the use thereof, the glycosyltransferase catalyzes transglucosylation of maltotriose units under conditions which can be employed for the processing of foods or the like. Provided is a maltotriosyl transferase which acts on polysaccharides and oligosaccharides having ?-1,4 glucoside bonds, and has activity for transferring maltotriose units to saccharides, the maltotriosyl transferase acting on maltotetraose as substrate to give a ratio between the maltoheptaose production rate and maltotriose production rate of 9:1 to 10:0 at any substrate concentration ranging from 0.67 to 70% (w/v).Type: GrantFiled: March 20, 2010Date of Patent: October 1, 2013Assignee: Amano Enzyme Inc.Inventors: Masamichi Okada, Shotaro Yamaguchi, Miho Nagaya
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Publication number: 20130252308Abstract: The present invention relates to glycerol-3-phosphate acyltransferases, polynucleotides encoding the same, etc. The present invention provides a polynucleotide comprising the nucleotide sequence of e.g., SEQ ID NO: 1 or 4, a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2, an expression vector and transformant comprising the polynucleotide, a method for producing food, etc. using the transformant, food, etc. produced by the method, and so on.Type: ApplicationFiled: September 21, 2010Publication date: September 26, 2013Applicant: SUNTORY HOLDINGS LIMITEDInventor: Misa Ochiai
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Patent number: 8541220Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.Type: GrantFiled: December 22, 2008Date of Patent: September 24, 2013Assignee: Verenium CorporationInventors: David P. Weiner, Ellen G. Burke, Peter Luginbuhl, Analia Bueno, Joslin M. Cuenca, Mervyn L. De Souza, Sherry Kollmann
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Patent number: 8541218Abstract: The present invention is directed to a mutant thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The ligase of the present invention is a mutant of a wild-type thermostable ligase having a histidine adjacent a KXDG motif, where the mutant thermostable ligase has a mutation in its amino sequence where the histidine adjacent the KXDG motif in the wild-type thermostable ligase is replaced with an arginine, and wherein X is any amino acid. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.Type: GrantFiled: June 24, 2005Date of Patent: September 24, 2013Assignee: Cornell Research Foundation, Inc.Inventors: Francis Barany, Weiguo Cao, Jie Tong
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ALPHA 1-3 N-GALACTOSYLSTRANSFERASE WITH ALTERED DONOR SPECIFICITIES, COMPOSITIONS AND METHODS OF USE
Publication number: 20130243690Abstract: The invention generally features compositions and methods based on the structure-based design of alpha 1-3 N-Acetylgalactosaminyltransferase (alpha 3 GalNAc-T) enzymes from alpha 1-3galactosyltransferase (a3Gal-T) that can transfer 2?-modified galactose from the corresponding UDP-derivatives due to substitutions that broaden the alpha 3Gal-T donor specificity and make the enzyme a3 GalNAc-T.Type: ApplicationFiled: April 11, 2013Publication date: September 19, 2013Applicant: Government of the United Sates of America, as represented by the Secretary Department of HealthInventors: Pradman K. Qasba, Boopathy Ramakrishnan, Elizabeth Boeggeman, Marta Pasek -
Publication number: 20130244287Abstract: A process for enzymatic preparation of a highly linear poly(? 1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to a highly linear poly(? 1, 3 glucan) in high titers. Hydrolyzed poly(? 1, 3 glucan) is used as the primer for the gtfJ enzyme reaction resulting in the formation of highly linear poly(? 1, 3 glucan).Type: ApplicationFiled: September 7, 2012Publication date: September 19, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: JOHN P. O'BRIEN, Mark S. Payne
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Patent number: 8535920Abstract: The present invention relates to a process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin by converting a substituted ?-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same.Type: GrantFiled: November 20, 2006Date of Patent: September 17, 2013Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Nao Funakoshi, Yusuke Amino, Shigeru Kawahara, Tadashi Takemoto
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Patent number: 8530214Abstract: The present invention relates to a polynucleotide encoding a monomer of an I-CreI variant said monomer comprising mutations in the amino acid sequence of SEQ ID NO: 34, wherein said mutations include: (i) at least one and up to eleven amino acid substitutions from residue S22 to Q44 said substitutions selected from the group consisting of substitutions at positions I24, Q26, K28, N30, S32, Y33, Q38, S40 and T42; and (ii) at least one and up to six amino acid substitutions from residue Y66 to I77 said substitutions selected from the group consisting of substitutions at positions Y66, R68, R70, V73 and I77; and wherein said monomer when in dimeric form binds and cleaves DNA.Type: GrantFiled: June 3, 2011Date of Patent: September 10, 2013Assignee: Cellectis S.A.Inventors: Sylvain Arnould, Patrick Chames, Philippe Duchateau, Jean-Charles Epinat, Emmanuel Lacroix, Frederic Paques
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Publication number: 20130230900Abstract: The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids.Type: ApplicationFiled: May 6, 2013Publication date: September 5, 2013Applicant: PIONEER HI BRED INTERNATIONAL INCInventors: Kanwarpal Singh Dhugga, Haiyin Wang, Dwight Tomes, Timothy George Helentjaris
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Patent number: 8524483Abstract: The object of the present invention is to provide a method for efficiently producing vinegar that contains a higher concentration of acetic acid, wherein a gene involved in the acetic acid fermentation ability is obtained, the acetic acid fermentation ability of an acetic acid bacterium is improved by reducing or deleting the function of the protein encoded by the gene. An acetic acid bacterium with a remarkably improved acetic acid fermentation ability was obtained by obtaining genes encoding an acyl homoserine lactone synthase and an acyl homoserine lactone receptor-type transcription factor that are involved in the quorum-sensing system in the acetic acid bacterium, and modifying the genes so as to reduce or delete the function of the quorum-sensing system. Further provided is a method for more efficiently producing vinegar containing a higher concentration of acetic acid by using the acetic acid bacterium.Type: GrantFiled: January 18, 2008Date of Patent: September 3, 2013Assignee: Mizkan Group CorporationInventor: Aya Iida
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Patent number: 8524478Abstract: Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.Type: GrantFiled: July 25, 2008Date of Patent: September 3, 2013Assignee: LG Chem, Ltd.Inventors: Si Jae Park, Taek Ho Yang, Hye Ok Kang, Sang Hyun Lee, Eun Jung Lee, Tae Wan Kim
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Patent number: 8524485Abstract: Methods for increasing C18 to C20 elongation conversion efficiency and/or ?4 desaturation conversion efficiency in long-chain polyunsaturated fatty acid [“LC-PUFA”]-producing recombinant oleaginous microbial host cells are provided herein, based on over-expression of acyl-CoA:lysophospholipid acyltransferases [“LPLATs”] (e.g., Ale1, LPAAT, LPCAT). Production host cells and oils produced by the methods of the invention are also claimed.Type: GrantFiled: June 14, 2010Date of Patent: September 3, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Publication number: 20130224175Abstract: This invention relates to a method of producing a recombinant enzyme, more particularly, this invention relates to a method of producing water soluble enzymatically active recombinant glycine N-acyltransferase (GLYAT (E.G. 2.1.3.13)), including the steps of providing a suitable expression host; preparing a vector including a gene for expressing GLYAT in the expression host to form an expression piasmid; transforming the host with the expression piasmid to form an expression system; expressing the GLYAT gene in the expression system; and separating the expressed GLYAT from the expression system.Type: ApplicationFiled: August 24, 2011Publication date: August 29, 2013Applicant: North-West UniversityInventors: Lodewyk Jacobus Mienie, Alberdina Aike Van Dijk, Christoffel Petrus Stephanus Badenhorst, Rencia Van Der Sluis
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Patent number: 8518678Abstract: Screening of fatty acid fed bacteria which are not natural butanol producers identified increased membrane cyclopropane fatty acid as providing improved butanol tolerance. Increasing expression of cyclopropane fatty acid synthase in the presence of the enzyme substrate that is either endogenous to the cell or fed to the cell, increased butanol tolerance. Bacterial strains with increased cyclopropane fatty acid synthase and having a butanol biosynthetic pathway are useful for production of butanol.Type: GrantFiled: December 9, 2008Date of Patent: August 27, 2013Assignee: Butamax(TM) Advanced Biofuels LLCInventors: Dennis Flint, Robert A. Larossa, Vasantha Nagarajan, Tina K. Van Dyk, Rick W. Ye
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Publication number: 20130217068Abstract: The present invention relates to nucleic acid and amino acid sequences from Akkermansia muciniphila and from Bacteroides fragilis, coding for/representing novel alpha-1,3-fucosyltransferases. The invention also provides uses and methods for using the alpha-1,3-fucosyltransferases to generate fucosylated products, such as oligosaccharides, (glyco)proteins, or (glyco)lipids, in particular of 3-fucosyllactose.Type: ApplicationFiled: April 10, 2013Publication date: August 22, 2013Applicant: JENNEWEIN BIOTECHNOLOGIE GMBHInventor: JENNEWEIN BIOTECHNOLOGIE GMBH
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Publication number: 20130217090Abstract: Novel crystal structures of human and murine glutaminyl cyclase (QC, EC 2.3.2.5), methods of preparing the crystals, as well as the use of said crystal structures for identifying inhibitors of human and murine glutaminyl cyclase.Type: ApplicationFiled: February 26, 2013Publication date: August 22, 2013Applicant: PROBIODRUG AGInventor: PROBIODRUG AG
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Patent number: 8512991Abstract: The invention relates generally to beta (1,4)-galactosyltransferase I mutants having altered donor and acceptor specificities, and methods of use thereof. In addition, the invention relates to methods for synthesizing oligosaccharides using the beta (1,4)-galactosyltransferase I mutants and to using the beta (1,4)-galactosyltransferase I mutants to conjugate agents, such as therapeutic agents or diagnostic agents, to acceptor molecules.Type: GrantFiled: August 22, 2007Date of Patent: August 20, 2013Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Pradman K. Qasba, Boopathy Ramakrishnan, Elizabeth Boeggeman
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Publication number: 20130210092Abstract: A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.Type: ApplicationFiled: September 28, 2011Publication date: August 15, 2013Applicant: Kaneka CorporationInventors: Toru Nagasawa, Toyokazu Yoshida, Kouichi Ishida, Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
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Patent number: 8507227Abstract: The present invention relates to a method for the large scale in vivo synthesis of sialylated oligosaccharides, culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as lactose, wherein said microorganism comprises heterologous genes encoding a CMP-Neu5Ac synthetase, a sialic acid synthase, a GlcNAc-6-phosphate 2 epimerase and a sialyltransferase, and wherein the endogenous genes coding for sialic acid aldolase (NanA) and for ManNac kinase (NanK) have been deleted or inactivated. The invention also relates to these micoorganisms which are capable of producing internally activated sialic acid.Type: GrantFiled: March 7, 2007Date of Patent: August 13, 2013Assignee: Centre National de la Recherche Scientifique (CNRS)Inventor: Eric Samain
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Patent number: 8507217Abstract: A new approach for increase of ethanol yield during alcoholic fermentation via decrease of biomass accumulation by using ATP degrading enzymes is described. The part of the Saccharomyces cerevisiae SSB1 gene coding for cytosolic ATPase domain of ribosome associated chaperon cloned into expression cassette under control of the glycerol-3-phosphate dehydrogenase gene (GPD1) promoter was introduced into the S. cerevisiae BY4742 strain. The recombinant strains were tested for their ability to grow and produce ethanol during glucose anaerobic and aerobic cultivations. Strains overexpressing ATPase domain of SSB1 possessed decreased concentration of intracellular ATP. They accumulated elevated amounts of ethanol and were characterized by decreased biomass accumulation as compared to the wild-type strain under both anaerobic and aerobic conditions. Similarly, the apyrase gene apy from E. coli encoding ATP/ADP hydrolyzing phosphatase and ATPase domain of SSB1 gene of S.Type: GrantFiled: June 28, 2010Date of Patent: August 13, 2013Assignee: Archer Daniels Midland CompanyInventors: Kostyantyn V. Dmytruk, Marta V. Semkiv, Andriy Sibirny
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Publication number: 20130205444Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.Type: ApplicationFiled: February 15, 2013Publication date: August 8, 2013Applicant: E.I. du Pont de Nemours and CompanyInventor: E.I. du Pont de Nemours and Company
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Publication number: 20130205441Abstract: This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.Type: ApplicationFiled: February 1, 2013Publication date: August 8, 2013Applicant: DOW AGROSCIENCES LLCInventor: Dow Agrosciences LLC
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Publication number: 20130198897Abstract: The present invention is directed to targeting genes and genomes, modifying the activity of enzymes and protein expression in plants. In particular, the present invention relates to methods for reducing the activity of one or more endogenous glycosyltransferases such as N-acetylglucosaminyltransferase, ?(1,2)-xylosyltransferase and a(1,3)-fucosyl-transferase in a plant cell and to plants obtained by said method.Type: ApplicationFiled: March 22, 2011Publication date: August 1, 2013Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Karen Keiko Oishi, Dionisius Elisabeth Antonius Florack, Prisca Campanoni, Carlo Massimo Pozzi, Jeremy Catinot, Nicolas Joseph Marie Sierro, Nikolai Valeryevitch Ivanov
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Publication number: 20130196389Abstract: A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.Type: ApplicationFiled: September 28, 2011Publication date: August 1, 2013Applicant: KANEKA CORPORATIONInventors: Noriyuki Ito, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
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Publication number: 20130196384Abstract: A process for production of poly (? 1,3 glucan) from a renewable feedstock, for applications in fibers, films, and pulps. The effect of addition of tetraborate in increasing the yield of the desired end products, poly (? 1,3 glucan) and fructose, and decreasing formation of the undesired by-product leucrose.Type: ApplicationFiled: December 19, 2012Publication date: August 1, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventor: E I Du Pont De Nemours And Company
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Publication number: 20130196385Abstract: The present invention provides novel methods for preparing glycolipid products. Novel sialyltransferases are also disclosed.Type: ApplicationFiled: January 11, 2013Publication date: August 1, 2013Applicant: The Regents of the University of California (02307W)Inventor: The Regents of the University of California (02307W)
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Publication number: 20130196336Abstract: The embodiments relate to the diagnosis of disorders or dysfunctions characterised by autoimmune responses to a novel antigen, transglutaminase 6, by the detection of autoantibodies to the novel antigen.Type: ApplicationFiled: April 7, 2008Publication date: August 1, 2013Inventors: Marios Hadjivassilliou, Daniel Aeschlimann