Transferase Other Than Ribonuclease (2.) Patents (Class 435/193)
Transferring phosphorus containing group (e.g., kineases, etc.(2.7)) (Class 435/194)
  • Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene
    Patent number: 8759072
    Abstract: The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii) or (iii): (i) a protein consisting of an amino acid sequence shown in Sequence No. 1; or (ii) a protein consisting of an amino acid sequence shown in Sequence No. 5, or a protein consisting of an amino acid sequence shown in Sequence No. 6, or a protein consisting of an amino acid sequence shown in Sequence No. 7, or a protein consisting of an amino acid sequence shown in Sequence No.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: June 24, 2014
    Assignees: An Educational Foundation Kinki University, Dainihon Jochugiku Co., Ltd.
    Inventors: Kazuhiko Matsuda, Yukio Kikuta
  • Process for producing prenyl alcohols
    Patent number: 8759046
    Abstract: A method of producing a prenyl alcohol(s) by culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced and recovering the prenyl alcohol(s) from the resultant culture.
    Type: Grant
    Filed: April 1, 2008
    Date of Patent: June 24, 2014
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Chikara Ohto, Shusei Obata
  • -phosphatidic acid acyltransferase from
    Patent number: 8759610
    Abstract: The cloning and broad characterization of a lyso-phosphatidic acid acyltransferase (LPAT2) from Tropaeolum majus is described. The TmLPAT2 enables the production of plants, seeds and cells with enhanced oil and/or fatty acid content. In particular, recombinant TMLPAT2 increases levels of very long chain fatty acids (VLCFA), especially erucic acid, in plants, seeds and cells.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: June 24, 2014
    Assignee: National Research Council of Canada
    Inventors: David C. Taylor, Tammy Francis
  • Type I polyketide synthase extender units
    Patent number: 8759031
    Abstract: Novel extender units for Type I polyketide synthases are provided. Also provided are genes, compounds, and methods for generating these units, and for incorporation of the novel extender units into polyketides for the purpose of generating new structural derivatives of polyketide-containing products.
    Type: Grant
    Filed: April 9, 2008
    Date of Patent: June 24, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Michael G. Thomas, Jo Handelsman, Yolande Alia Chan, Angela M. Podevels
  • Prebiotic composition or pharmaceutical composition synthesized from catalytic domains producing highly ?-1,2 branched dextran
    Patent number: 8758744
    Abstract: The invention relates to an isolated polypeptide with an glycosyl transferase enzymatic activity for producing dextrans with .alpha.(1.fwdarw.2) sidechains, comprising at least one region for bonding to glucan and a catalytically active region situated beyond the region bonding to glucan. The invention further relates to polynucleotides coding for said enzymes and vectors containing the same.
    Type: Grant
    Filed: June 25, 2008
    Date of Patent: June 24, 2014
    Assignee: Institut National des Sciences Appliquees (INSA)
    Inventors: Sophie Anne Michèle Bozonnet, Magali Martine Claude Remaud-Simeon, René—Marc Lucien Willemot, Pierre Emmanuel Frédéric Monsan
  • Maize Cellulose Synthases and Uses Thereof
    Publication number: 20140173782
    Abstract: The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids.
    Type: Application
    Filed: February 21, 2014
    Publication date: June 19, 2014
    Applicant: Pioneer Hi Bred International Inc
    Inventors: Kanwarpal S Dhugga, Timothy George Helentjaris, Dwight Tomes, Haiyin Wang
  • DGAT GENES COMPRISING PLECKSTRIN HOMOLOGY DOMAINS AND METHODS OF USE FOR TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS
    Publication number: 20140162329
    Abstract: The present invention provides novel diacylglycerol acyltransferase (DGAT) genes comprising Pleckstrin Homology (PH) domains. The present invention also provides for recombinant cells, such as algae, transformed with acyltransferase genes, such as DGAT, comprising PH domains, and methods of using such recombinant cells to produce increased triglyceride levels.
    Type: Application
    Filed: December 6, 2012
    Publication date: June 12, 2014
    Applicant: ExxonMobil Research and Engineering Company
    Inventors: Jennifer Coppersmith, Rekha Seshadri, Toby Howard Richardson
  • ?-1,4-galactosyltransferase (CgtD) from
    Patent number: 8748135
    Abstract: ?-1,4-galactosyltransferase (CgtD) polypeptides, nucleic acids that encode the polypeptides, including a polypeptide from Campylobacter jejuni strain LIO87 have been isolated and characterized. A method of producing a galactosylated saccharide comprising contacting an acceptor saccharide containing a terminal galactose, a donor substrate comprising a galactose moiety and one of the CgtD polypeptides is described.
    Type: Grant
    Filed: November 28, 2012
    Date of Patent: June 10, 2014
    Assignee: National Research Council of Canada
    Inventors: Michel Gilbert, Warren Wakarchuk, Scott Houliston
  • Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene
    Patent number: 8748155
    Abstract: Amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin. A gene encoding a protein consisting of the amino acid sequence of SEQ ID NO: 1. A protein consisting of the amino acid sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: December 19, 2012
    Date of Patent: June 10, 2014
    Assignees: An Educational Foundation Kinki University, Dainihon Jochugiku Co., Ltd.
    Inventors: Kazuhiko Matsuda, Yukio Kikuta
  • Method for production of polylactate using recombinant microorganism
    Patent number: 8748137
    Abstract: It is an object of the present invention to provide a method for efficiently producing a polylactate via microbial fermentation with the use of a sugar as a starting material.
    Type: Grant
    Filed: October 27, 2009
    Date of Patent: June 10, 2014
    Assignee: Toyota Jidosha Kabushiki Kaisha
    Inventors: Shusei Obata, Hiromi Kambe, Masakazu Ito, Takashi Shimamura, Katsunori Kohda, Seiichi Taguchi
  • Producing methionine without N-acyl-methionine
    Patent number: 8748136
    Abstract: The present invention relates to a method for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulphur. The microorganism and/or the culture medium and/or the process parameters were modified in a way that the accumulation of the by-product N-acyl-methionine (NAM) is reduced. The isolation of methionine or its derivatives from the fermentation medium is also claimed.
    Type: Grant
    Filed: February 12, 2009
    Date of Patent: June 10, 2014
    Assignee: Metabolic Explorer
    Inventors: Rainer Figge, Philippe Soucaille, Gwénaëlle Bestel-Corre
  • Engineered nucleases and their uses for nucleic acid assembly
    Patent number: 8748146
    Abstract: Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA.
    Type: Grant
    Filed: April 19, 2008
    Date of Patent: June 10, 2014
    Assignee: Celexion, LLC
    Inventors: Shaun Lippow, Dasa Lipovsek, Patricia M. Aha
  • Compositions and Methods for Making Selenocysteine Containing Polypeptides
    Publication number: 20140154744
    Abstract: Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.
    Type: Application
    Filed: July 11, 2012
    Publication date: June 5, 2014
    Applicant: YALE UNIVERSITY
    Inventors: Dieter Soll, Caroline Aldag, Michael Hohn
  • PROCESS FOR SEPARATING PROTEINS FIBRINOGEN, FACTOR XIII AND BIOLOGICAL GLUE FROM A SOLUBILIZED PLASMA FRACTION AND FOR PREPARING LYOPHILISED CONCENTRATES OF SAID PROTEINS
    Publication number: 20140154231
    Abstract: Process for separating proteins fibrinogen, Factor XIII and biological glue from a solubilized plasma fraction and for preparing lyophilised concentrates of said proteins.
    Type: Application
    Filed: October 17, 2013
    Publication date: June 5, 2014
    Applicant: Laboratoire Francais Du Fractionnement Et Des Biotechnologies
    Inventors: Michel NOGRE, Pierre PORTE, Michel TELLIER
  • MODIFIED POLYNUCLEOTIDES FOR TREATING PROTEIN DEFICIENCY
    Publication number: 20140148502
    Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.
    Type: Application
    Filed: February 3, 2014
    Publication date: May 29, 2014
    Applicant: MODERNA THERAPEUTICS, INC.
    Inventors: Stephane Bancel, Tirtha Chakraborty, Antonin de Fougerolles, Susan Whoriskey
  • Traceless Ubiquitination
    Publication number: 20140148576
    Abstract: The invention related to a tRNA synthetase capable of binding delta-substituted lysine, wherein said tRNA synthetase comprises amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS, wherein said sequence comprises 5 or fewer substitutions within the amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS; and wherein said synthetase comprises W at amino acid position 349 relative to MbPyIRS.
    Type: Application
    Filed: June 22, 2012
    Publication date: May 29, 2014
    Applicant: Medical Research Council
    Inventors: Jason Chin, Satpal Virdee
  • NEOEPITOPE DETECTION OF DISEASE USING PROTEIN ARRAYS
    Publication number: 20140141992
    Abstract: A biosensor for use in detecting the presence of diseases, the biosensor comprising a detector for detecting a presence of at least one marker indicative of a specific disease. A method of determining efficacy of a pharmaceutical for treating a disease or staging disease by administering a pharmaceutical to a sample containing markers for a disease, detecting the amount of at least one marker of the disease in the sample, and analyzing the amount of the marker in the sample, whereby the amount of marker correlates to pharmaceutical efficacy or disease stage. Markers for gynecological disease. An immune-imaging agent comprising labeled antibodies, whereby the labeled antibodies are isolated and reactive to proteins overexpressed in vivo. Informatics software for analyzing the arrays, the software including analyzing means for analyzing the arrays.
    Type: Application
    Filed: November 13, 2013
    Publication date: May 22, 2014
    Applicant: Wayne State University
    Inventors: Michael Tainsky, Sorin Draghici, Madhumita Chatterjee
  • YEAST CELL CAPABLE OF CONVERTING SUGARS INCLUDING ARABINOSE AND XLOSE
    Publication number: 20140141473
    Abstract: Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.
    Type: Application
    Filed: April 20, 2012
    Publication date: May 22, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom, Panagiotis Sarantinopoulos, Wilbert Herman Marie Heijne, Aldo Greeve
  • Methods for modifying cell surface glycans
    Patent number: 8728810
    Abstract: Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein.
    Type: Grant
    Filed: September 26, 2011
    Date of Patent: May 20, 2014
    Inventor: Robert Sackstein
  • DNA polymerases with increased 3?-mismatch discrimination
    Patent number: 8722379
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: June 17, 2011
    Date of Patent: May 13, 2014
    Assignee: Roche Medical Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Glycosyltransferase, novel glycosyltransferase gene, and novel sugar donor compound
    Patent number: 8722868
    Abstract: An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein R1 is independently selected from hydrogen, or C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO2, and SO2, n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a ? bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.
    Type: Grant
    Filed: January 21, 2010
    Date of Patent: May 13, 2014
    Assignee: National University Corporation Tokyo University of Agriculture and Technology
    Inventors: Yoshihiro Ozeki, Nobuhiro Sasaki, Kazuo Nagasawa, Masayuki Tera, Yuki Matsuba, Haruka Nakamura, Yutaka Abe
  • Methods for synthesizing sugar chains using ?1,3-N-acetylglucosaminyltransferase
    Patent number: 8722366
    Abstract: The present invention provides a novel polypeptide having a ?1,3-N-acetylglucosaminyltransferase activity; a method for producing the polypeptide; a DNA which encodes the polypeptide; a recombinant vector into which the DNA is inserted; a transformant comprising the recombinant vector; a method for producing a sugar chain or complex carbohydrate, using the polypeptide; a method for producing a sugar chain or complex carbohydrate, using the transformant; an antibody which recognizes the polypeptide; a method for screening a substance which changes the expression of the gene which encodes the polypeptide; and a method for screening a substance which changes the activity of the polypeptide.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: May 13, 2014
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Katsutoshi Sasaki, Norihiko Shiraishi, Ayumi Natsume, Yoji Yamada, Satoshi Nakagawa, Susumu Sekine
  • Metabolically engineered for enhanced production of sialic acid
    Patent number: 8722365
    Abstract: A metabolically engineered E. coli strain which produces sialic acid and a method of making said strain. In the engineered E. coli cells, the nanT (sialic acid transporter) and nanA (sialic acid adolase) genes are inactivated, and the neuC and neuB genes of sialic acid biosynthesis in Neisseria meningitidis group B are introduced and overexpressed in the nanT? nanA? E. coli cell. In addition, the glucosamine synthase gene, glmS, of E. coli is co-overexpressed with neuB and neuC.
    Type: Grant
    Filed: September 26, 2007
    Date of Patent: May 13, 2014
    Assignee: Syracuse University
    Inventors: Christopher N. Boddy, Benjamin R. Lundgren
  • Mapping Cytosine Modifications
    Publication number: 20140127678
    Abstract: Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.
    Type: Application
    Filed: March 14, 2013
    Publication date: May 8, 2014
    Inventors: Shengxi Guan, Nan Dai, Zhenyu Zhu, Ivan R. Correa, Jr., Aine Quimby, Janine Borgaro
  • VACCINES FOR PREVENTION AND TREATMENT OF TUBERCULOSIS
    Publication number: 20140127251
    Abstract: The present invention provides new immunological compositions and vaccines comprising selected M. tuberculosis antigens and antigenic peptides as well as nucleic acids encoding said antigens for use in the prevention, prophylaxis and treatment of mycobacterial infection, especially tuberculosis. In particular the invention provides recombinant BCG based vaccines in which one or more of the selected M. tuberculosis antigens are over expressed. The invention further provides isolated peptides for use in methods for diagnosing, characterizing, or classifying mycobacterial infections.
    Type: Application
    Filed: April 11, 2012
    Publication date: May 8, 2014
    Applicant: ALARUM DEVELOPMENT LTD.
    Inventor: Markus Maeurer
  • Cell-free synthesis of proteins containing unnatural amino acids
    Patent number: 8715958
    Abstract: Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of polypeptides containing unnatural amino acids at one or more specified residues of the polypeptide.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: May 6, 2014
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Aaron R. Goerke, James Robert Swartz
  • Inhibition of DNA polymerases to augment chemotherapeutic and antimicrobial agents
    Patent number: 8715927
    Abstract: Disclosed herein is the identification of human DNA polymerase ? (pol ?) as the polymerase that mediates repair of DNA containing interstrand cros slinks (ICLs). The mechanism of action of a number of chemotherapeutic and antimicrobial agents is the induction of ICLs. Thus, provided herein is a method of enhancing the efficacy of a chemotherapeutic or antimicrobial agent in a subject, including selecting a subject in need of treatment with an ICL -inducing agent and administering to the subject an ICL-inducing agent and a therapeutically effective amount of an inhibitor of pol ?. Also provided is a composition for treating a hyperproliferative disease, an autoimmune disease or an infectious disease, comprising an ICL-inducing agent and an amount of an inhibitor of pol ? sufficient to enhance the efficacy of the ICL-inducing agent. Further provided is a method of identifying a DNA polymerase inhibitor.
    Type: Grant
    Filed: April 22, 2009
    Date of Patent: May 6, 2014
    Assignee: Oregon Health & Science University
    Inventors: R. Stephen Lloyd, Irina G. Minko, Amanda K. McCullough
  • Ethanol production from ocean biomass
    Patent number: 8715977
    Abstract: An object of the present invention is to provide a method for producing ethanol from polysaccharide alginate contained in a large amounts in brown algae, using the alginate assimilation capacity of the Sphingomonas sp. strain A1 and the strong ethanol production capacity of bacteria such as Zymomonas mobilis. Specifically, a method for producing ethanol using alginate as a raw material comprises causing genes encoding proteins and enzymes involved in Sphingomonas sp. strain A1-derived alginate assimilation and genes encoding enzymes involved in ethanol production to co-exist in a single microorganism and then culturing the microorganism in a medium containing alginate.
    Type: Grant
    Filed: August 25, 2010
    Date of Patent: May 6, 2014
    Assignees: Kyoto University, Maruha Nichiro Holdings, Inc.
    Inventors: Kousaku Murata, Wataru Hashimoto, Shigeyuki Kawai, Hiroshi Oda, Keishi Iohara, Bunzo Mikami, Hiroyuki Takeda, Fuminori Yoneyama, Akihito Ochiai
  • METHODS, SYSTEMS AND COMPOSITIONS REGARDING MULTIPLEX CONSTRUCTION PROTEIN AMINO-ACID SUBSTITUTIONS AND IDENTIFICATION OF SEQUENCE-ACTIVITY RELATIONSHIPS, TO PROVIDE GENE REPLACEMENT SUCH AS WITH TAGGED MUTANT GENES, SUCH AS VIA EFFICIENT HOMOLOGOUS RECOMBINATION
    Publication number: 20140121118
    Abstract: A method is provided to construct libraries of nucleic acids that comprise a plurality of mutations, each of which may be identified with particularity, such as following a selection or screening.
    Type: Application
    Filed: November 14, 2013
    Publication date: May 1, 2014
    Inventor: Joseph R. Warner
  • ENHANCEMENT OF IN VITRO TRANSLATION BY NANOPARTICLE CONJUGATES
    Publication number: 20140120575
    Abstract: Provided herein are kits and methods suitable for enhancing in vitro translation of a nuclei acid sequence of interest.
    Type: Application
    Filed: April 23, 2013
    Publication date: May 1, 2014
    Inventor: Massachusetts Institute of Technology
  • Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
    Patent number: 8709772
    Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., damino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H2O+acceptor<=>a 2-oxo acid+NH3+reduced acceptor.
    Type: Grant
    Filed: December 31, 2008
    Date of Patent: April 29, 2014
    Assignee: Verenium Corporation
    Inventors: David P. Weiner, Peter Luginbuhl, Analia Bueno, Joslin Cuenca, Erin Marasco
  • Use of endogenous promoters in genetic engineering of
    Patent number: 8709766
    Abstract: The present disclosure is directed to novel polynucleotide sequences for use in Nannochloropsis gaditana. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein N. gaditana promoters/control regions are operatively linked to N. gaditana or non-N. gaditana coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into N. gaditana and can recombine into the N. gaditana genome. Expression from these polynucleotide sequences and expression constructs can enhance N. gaditana biomass and/or lipid biosynthesis. Also disclosed are methods for modifying N. gaditana, for example by stably transforming N. gaditana with nucleic acid sequences, growing the modified N. gaditana, and obtaining biomass and biofuels from the modified N. gaditana.
    Type: Grant
    Filed: October 17, 2012
    Date of Patent: April 29, 2014
    Assignee: Colorado School of Mines
    Inventors: Randor Radakovits, Robert Jinkerson, Matthew Posewitz
  • Cross-linked compositions
    Patent number: 8703117
    Abstract: Improved compositions comprising a cross-linkable protein or polypeptide, and a non-toxic material which induces cross-linking of the cross-linkable protein. The compositions are optionally and preferably prepared in a non-phosphate buffer solvent. Optionally and preferably, the cross-linkable protein includes gelatin and any gelatin variant or variant protein as described herein. Optionally and preferably, the non-toxic material comprises transglutaminase (TG), which may optionally comprise any type of calcium dependent or independent transglutaminase, which may for example optionally be a microbial transglutaminase (mTG).
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: April 22, 2014
    Assignee: Lifebond Ltd.
    Inventors: Orahn Preiss Bloom, Ishay Attar, Natalie Iram, Chagai Komlos, Meir Haber
  • Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity
    Patent number: 8703459
    Abstract: Disclosed are mutants of galactosyltransferases that can catalyze formation of oligosaccharides in the presence of magnesium; mutants of galactosyltransferases having altered donor and acceptor specificity which can catalyze formation of oligosaccharides in the presence of magnesium; methods and compositions that can be used to synthesize oligosaccharides; methods for increasing the immunogenicity of an antigen; and methods to stabilize platelets.
    Type: Grant
    Filed: December 6, 2004
    Date of Patent: April 22, 2014
    Assignee: The United States of America, as represented by the Secretary, Department of Health & Human Services
    Inventors: Pradman Qasba, Elizabeth Boeggeman, Boopathy Ramakrishnan
  • Enzymatic transamination of cyclopamine analogs
    Patent number: 8703448
    Abstract: The invention relates to the preparation of amino-cyclopamines by the enzymatic transamination of a corresponding keto-cyclopamines in the presence of a cofactor and an amino donor.
    Type: Grant
    Filed: July 1, 2011
    Date of Patent: April 22, 2014
    Assignee: Infinity Pharmaceuticals, Inc.
    Inventors: Brian C. Austad, Adilah Bahadoor, Jitendra D. Belani, Somarajannair Janardanannair, Charles W. Johannes, Gregg F. Keaney, Priscilla L. White, Sheldon L. Wallerstein
  • Modified lecithin-cholesterol acyltransferase enzymes
    Patent number: 8703926
    Abstract: The invention provides compounds, pharmaceutical compositions and methods for treating atherosclerosis, coronary heart disease, thrombosis, and for decreasing or prevention of accumulation of cholesterol in a subject by modifying LCAT polypeptide.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: April 22, 2014
    Assignee: Amgen Inc.
    Inventors: Mingyue Zhou, Wenyan Shen, Bei Shan, Margrit Schwarz, David Park Meininger, Tom Boone
  • Method for the production of an additive for the enzymatic decomposition of mycotoxins, additive, and use thereof
    Patent number: 8703460
    Abstract: In a method for producing an additive for the enzymatic degradation of mycotoxins, in particular fumonisins, it is provided that at least one nucleic acid sequence of genes corresponding to sequences ID Nos. 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 is provided, the at least one nucleic acid sequence is expressed in prokaryotic or eukaryotic host cells, and at least one thus prepared enzyme corresponding to sequences ID Nos. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or at least one complete recombinant host organism optionally along with a cosubstrate, are used in a vegetable raw material.
    Type: Grant
    Filed: September 18, 2009
    Date of Patent: April 22, 2014
    Assignee: Erber Aktiengesellschaft
    Inventors: Wulf-Dieter Moll, Doris Hartinger, Karin Grieβler, Eva Maria Binder, Gerd Schatzmayr
  • Bioabsorbable polymer containing 2-hydroxyacid monomers
    Patent number: 8703449
    Abstract: Genetically engineered organisms for production of PHA copolymers containing 2-hydroxyacid monomers and the methods of making and using thereof have been developed. The copolymers containing 2-hydroxyacid monomers can be synthesized via biosynthesis by the action of a PHA polymerase in a living cell. By changing the genetic background of the cells, one can control specific metabolic pathways allowing control of the level of glycolic acid co-monomer in the PHA polymer.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: April 22, 2014
    Assignee: Metabolix, Inc.
    Inventors: David P. Martin, Frank A. Skraly
  • System and method for the production of recombinant glycosylated proteins in a prokaryotic host
    Patent number: 8703471
    Abstract: A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains the genetic information required for the expression of one or more recombinant target proteins. The metabolic apparatus preferably comprises specific glycosyltransferases for the assembly of the oligosaccharide on a lipid carrier and an OTase that covalently links this oligosaccharide to specific residues of the desired protein.
    Type: Grant
    Filed: July 21, 2008
    Date of Patent: April 22, 2014
    Assignee: ETH Zürich
    Inventors: Markus Aebi, Michael Wacker
  • Process and microorganisms for production of lipids from lignocellulosic wastes or residues
    Patent number: 8697403
    Abstract: Disclosed are processes for producing lipids for biofuel or lubricant and Streptomyces bacteria used in the processes. The processes include steps whereby bacterial cells of the germs Streptomyces are cultivated in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), wherein the organic waste(s) or residue(s) comprise lignocellulosic waste(s) or lignocellulosic residue(s), recovering lipids from the cells of the bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: April 15, 2014
    Assignee: Neste Oil Oyj
    Inventors: Maria Holmback, Miina Lehesto, Perttu Koskinen, Johan-Fredrik Selin
  • Lysosomotropic inhibitors of acid ceramidase
    Patent number: 8697379
    Abstract: The presently disclosed subject matter provides compounds of the formula, formula (Ia): and pharmaceutically acceptable salts thereof, wherein R1, R2, R3, R4, R5, X, Y, and n are as defined herein. Also disclosed are methods for making the compounds of the formula as set forth hereinabove, their use in inhibiting acid ceramidase and ceramidase-related activity, and their use as drugs and prodrugs in the treatment and/or prevention of diseases associated with undesirable ceramidase or sphingosine kinase activity, including, but not limited to, cancer, cancer metastasis, atherosclerosis, stenosis, inflammation, asthma, and atopic dermatitis.
    Type: Grant
    Filed: November 6, 2009
    Date of Patent: April 15, 2014
    Assignee: MUSC Foundation for Research Development
    Inventors: Alicja Bielawska, Aiping Bai, Zdzislaw M. Szulc, Yusuf A. Hannun, James S. Norris, Liu Xiang
  • Plants having increased yield-related traits and a method for making the same
    Patent number: 8697947
    Abstract: The present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by modulating expression in a plant of a nucleic acid sequence encoding a yield increasing polypeptide selected from the group consisting of: an AT-hook motif nuclear localized 19/20 (AHL19/20), a GRP (Growth Regulating Protein) (wherein said GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and an alanine aminotransferase (AAT) polypeptide.
    Type: Grant
    Filed: July 21, 2008
    Date of Patent: April 15, 2014
    Assignee: BASF Plant Science GmbH
    Inventors: Yves Hatzfeld, Valerie Frankard
  • Compositions and methods for producing gamma-carboxylated proteins
    Patent number: 8697440
    Abstract: The present invention relates to methods and tools for producing large quantities of gamma-carboxylated protein comprising: (i) culturing a cell adapted to express a protein which requires gamma-carboxylation and ?-glutamyl carboxylase in a ratio of at least 10:1, under conditions suitable for expression of both proteins, and (ii) isolating gamma-carboxylated protein.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: April 15, 2014
    Assignee: Medimmune Limited
    Inventors: Christel Fenge, Ann Lövgren, Anders Thelin
  • Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof
    Patent number: 8697395
    Abstract: Use of meganucleases for cleaving DNA in a non-human or an isolated human cell and, in some instances, inducing homologous recombination in said cells and to its application for genome engineering and gene therapy.
    Type: Grant
    Filed: July 24, 2012
    Date of Patent: April 15, 2014
    Assignee: Cellectis S.A.
    Inventors: Sylvain Arnould, Patrick Chames, Phillippe Duchateau, Jean-Charles Epinat, Emmanuel Lacroix, Frederic Paques
  • Therapy of neurological inflammatory diseases with (5'-deoxy-5'-adenosyl) cobamamide, recombinant human growth hormone, interleukins IL-1, IL-6, IL-11, epidermal growth factor, and physiotherapy
    Patent number: 8691790
    Abstract: A novel etiological hypothesis for Multiple Sclerosis (MS) is proposed describing autoimmune attack of ATP: Cob(I)alamin adenosyltransferase (ATR) thereby inhibiting synthesis of (5?-deoxy-5?-adenosyl)cobamide (referred to as 5?-deoxyadenosylcobalmin or AdoCbl) from Vitamin B 12 providing a basis for therapeutic design and diagnostic methods. Pharmaceutical compositions for therapy of MS, inflammatory neurological diseases and neurodegenerative diseases utilizing AdoCbl, growth hormones, immunomodulators, interleukins, other therapeutic agents, and physiotherapy are also described.
    Type: Grant
    Filed: July 27, 2009
    Date of Patent: April 8, 2014
    Inventor: James Layne Boucher
  • TYPE II RESTRICTION MODIFICATION SYSTEM METHYLATION SUBUNIT OF ALICYCLOBACILLUS ACIDOCALDARIUS
    Publication number: 20140093936
    Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.
    Type: Application
    Filed: October 17, 2013
    Publication date: April 3, 2014
    Applicant: BATTELLE ENERGY ALLIANCE, LLC
    Inventors: Brady D. Lee, Deborah T. Newby, Jeffrey A. Lacey, David N. Thompson, Vicki S. Thompson, William A. Apel, Francisco F. Roberto, David W. Reed
  • Recombinant capable of producing polylactic acid or polylatic acid compolymer, and method for producing polylactic acid or polylatic acid copolymer using same
    Patent number: 8685701
    Abstract: Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: April 1, 2014
    Assignee: LG Chem, Ltd.
    Inventors: Taek Ho Yang, Si Jae Park, Eun Jeong Lee, Hye Ok Kang, Tae Wan Kim, Sang Hyun Lee
  • Process for producing recombinant glycoproteins by culturing a mutant strain
    Patent number: 8685671
    Abstract: The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ?-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: April 1, 2014
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Hyun Ah Kang, Jeong Seok Park, Moo Woong Kim, Eun Jung Kim, Hye Yun Moon, Doo Byoung Oh, Joo Hyung Heo, Sang Ki Rhee
  • Enzyme catalysts for diels-alder reactions
    Patent number: 8688427
    Abstract: The present invention provides enzyme catalysts for Diels-Alder reactions, including intermolecular Diels-Alder reactions, as well as protein scaffolds for making such enzyme catalysts. In other aspects, the invention provides methods of making the enzyme catalysts, including by de novo computational design. The present invention thereby provides enzyme catalysts capable of catalyzing a desired Diels-Alder reaction, including with a specified or desired stereo-selectivity.
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: April 1, 2014
    Assignee: University of Washington
    Inventors: David Baker, Alexandre Zangellini, Justin Siegel, Jennifer St. Clair
  • Chondroitin synthase, method for producing the same and method for producing saccharide chain-extended chondroitin
    Patent number: 8685674
    Abstract: A vector of the present invention has DNA encoding a protein or a product having the same effect as the protein, the protein containing an amino acid sequence from amino acid numbers 47 to 802 in SEQ. ID. NO:2. Expression of the DNA gives human chondroitin synthase. By using human chondroitin synthase, it is possible to produce a saccharide chain having a repeating disaccharide unit of chondroitin. The DNA or part thereof may be used as a probe for hybridization for the human chondroitin synthase.
    Type: Grant
    Filed: October 9, 2012
    Date of Patent: April 1, 2014
    Assignee: Glytech, Inc.
    Inventors: Kazuyuki Sugahara, Hiroshi Kitagawa