Hydrolase (3. ) Patents (Class 435/195)
Acting on ester bond (3.1) (Class 435/196)
Acting on glycosyl compound (3.2) (Class 435/200)
Acting on peptide bond (e.g., thromboplastin, leucine amino-peptidase, etc., (3.4)) (Class 435/212)
Acting on carbon to nitrogen bond other than peptide bond (3.5) (Class 435/227)
  • Phosphoserine phosphatase of coryneform bacteria and variants thereof
    Patent number: 7329523
    Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
    Type: Grant
    Filed: October 7, 2005
    Date of Patent: February 12, 2008
    Assignee: Ajinomoto Co., Inc.
    Inventors: Mikiko Suga, Yoko Asakura, Masakazu Sugimoto, Hisao Ito
  • Serine proteases from-gram-positive microorganisms
    Patent number: 7329526
    Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
    Type: Grant
    Filed: December 16, 2004
    Date of Patent: February 12, 2008
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Serine proteases from gram-positive microorganisms
    Patent number: 7329527
    Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
    Type: Grant
    Filed: December 16, 2004
    Date of Patent: February 12, 2008
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Serine proteases from gram-positive microorganisms
    Patent number: 7329525
    Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
    Type: Grant
    Filed: December 16, 2004
    Date of Patent: February 12, 2008
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • BGL4 beta-glucosidase and nucleic acids encoding the same
    Patent number: 7320886
    Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.
    Type: Grant
    Filed: August 22, 2005
    Date of Patent: January 22, 2008
    Assignee: Genecor International, Inc.
    Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
  • Dihydroorotase extracted from plants
    Patent number: 7320877
    Abstract: The present invention relates to a DNA encoding a polypeptide with dihydroorotase (EC 3.5.2.3) activity. Also, the invention relates to the use of this nucleic acid for the generation of an assay system.
    Type: Grant
    Filed: September 2, 2000
    Date of Patent: January 22, 2008
    Assignee: BASF Aktiengesellschaft
    Inventors: Thomas Ehrhardt, Jens Lerchl, Marc Stitt Nigel, Rita Zrenner, Michael Schroeder
  • Serine proteases from gram-positive microorganisms
    Patent number: 7316920
    Abstract: The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
    Type: Grant
    Filed: December 16, 2004
    Date of Patent: January 8, 2008
    Assignee: Genencor International, Inc.
    Inventor: David A. Estell
  • Enhancement of enzyme activity through purification and immobilization
    Patent number: 7312056
    Abstract: An improved method of making an immobilized enzyme comprising (a) treating an immobilization support with an aqueous solution comprising a cross-linking agent and polymeric aldehyde species and active centre species to produce a modified support; (b) isolating the modified support; (c) treating an enzyme solution with the modified support to produce the immobilized enzyme, the improvement comprising treating the aqueous solution of cross-linking agent with an effective amount of a purifying agent to reduce the amount of the polymeric aldehyde species and active centre species.
    Type: Grant
    Filed: March 11, 2004
    Date of Patent: December 25, 2007
    Assignee: Immortazyme Company
    Inventors: Bradley A. Saville, Mikhail I. Khavkine
  • Alpha-amylase variants
    Patent number: 7306936
    Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
    Type: Grant
    Filed: May 15, 2002
    Date of Patent: December 11, 2007
    Assignee: Novozymes A/S
    Inventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff
  • Methods for producing ?-substituted carboxylic acids using nitrilases and strecker reagents
    Patent number: 7300775
    Abstract: In accordance with the present invention there are provided methods for producing enantiomerically pure ?-substituted carboxylic acids, such as, for example, ?-amino acids and ?-hydroxy acids, said method comprising combining an aldehyde or ketone with a cyanide and ammonia or an anmionium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the enantiomerically pure ?-substituted carboxylic acid, such as, for example, ?-amino acids and ?-hydroxy acids.
    Type: Grant
    Filed: December 28, 2000
    Date of Patent: November 27, 2007
    Assignee: Verenium Corporation
    Inventors: Darcy Madden, legal representative, David Paul Weiner, Jennifer Ann Chaplin, Dan E. Robertson, Mark Madden, deceased
  • Highly productive ?-amylases
    Patent number: 7297527
    Abstract: The invention relates to mutant ?-amylases that may be produced at high yield from recombinant microorganisms.
    Type: Grant
    Filed: March 12, 2004
    Date of Patent: November 20, 2007
    Assignee: Kao Corporation
    Inventors: Hiroyuki Araki, Keiji Endo, Hiroshi Hagihara, Kazuaki Igarashi, Yasuhiro Hayashi, Katsuya Ozaki
  • PR03580 polypeptides
    Patent number: 7294495
    Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
    Type: Grant
    Filed: April 24, 2002
    Date of Patent: November 13, 2007
    Assignee: Genentech, Inc.
    Inventors: Audrey Goddard, Paul J. Godowski, Austin L. Gurney, Victoria Smith, Colin K. Watanabe, William I. Wood
  • Anti-PRO3580 antibodies
    Patent number: 7294705
    Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
    Type: Grant
    Filed: April 24, 2002
    Date of Patent: November 13, 2007
    Assignee: Genetech, Inc.
    Inventors: Audrey Goddard, Paul J. Godowski, Austin L. Gurney, Victoria Smith, Colin K. Watanabe, William I. Wood
  • Method for efficiently producing transgenic plant using auxin precursor
    Patent number: 7294761
    Abstract: A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme which synthesizes auxin from an auxin precursor; (B) culturing the plant cell into which the genes are introduced by the vector, in the presence of an auxin precursor and/or an analogue thereof to thereby prepare a redifferentiated tissue, and detecting and selecting the redifferentiated tissues; and (C) culturing the redifferentiated tissue selected in (B) to redifferentiate a plant individual, and a vector for gene introduction into a plant, which comprises: a desired gene, and a selectable marker gene comprising an indoleacetamide hydrolase, iaaH, gene and an isopentenyl transferase, ipt, gene and being free of an tryptophan monooxygenase, iaaM, gene.
    Type: Grant
    Filed: July 25, 2003
    Date of Patent: November 13, 2007
    Assignee: Nippon Paper Industries Co., Ltd.
    Inventors: Etsuko Matsunaga, Koichi Sugita, Hiroyasu Ebinuma
  • Microorganisms and processes for enhanced production of pantothenate
    Patent number: 7291489
    Abstract: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
    Type: Grant
    Filed: July 3, 2003
    Date of Patent: November 6, 2007
    Assignee: BASF Aktiengesellschaft
    Inventors: R. Rogers Yocum, Thomas A. Patterson, Janice G. Pero, Theron Hermann
  • Rapid screening and analysis of present and potential nerve agents and their degradation products
    Patent number: 7291478
    Abstract: Methods are described for detecting organophosphorus compounds in a sample.
    Type: Grant
    Filed: March 29, 2005
    Date of Patent: November 6, 2007
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventor: Ilya Elashvili
  • Family GH-61 polypeptides
    Patent number: 7273738
    Abstract: The present invention relates to use of an anti-staling GH-61 polypeptide for preparing an edible product.
    Type: Grant
    Filed: October 1, 2003
    Date of Patent: September 25, 2007
    Assignee: Novozymes A/S
    Inventors: Kirk Matthew Schnorr, Sara Landvik, Tina Spendler, Lars Lehmann Hylling Christensen
  • Heparanase-like polypeptides
    Patent number: 7273744
    Abstract: The present invention provides a homologue to heparanase which is present in three splice variants.
    Type: Grant
    Filed: June 21, 2002
    Date of Patent: September 25, 2007
    Assignee: UCB Pharma S.A.
    Inventors: Edward Alexander McKenzie, Alasdair Craig Stamps, Jonathan Alexander Terrett, Kerry Louise Tyson
  • Polynucleotides encoding a 6-phosphogluconolactonase polypeptide from corynebacterium glutamicum
    Patent number: 7270984
    Abstract: Isolated nucleic acid molecules, designated sugar metabolism and oxidative phosphorylation (SMP) nucleic acid molecules, which encode novel SMP proteins from Corynebacterium glutamicum, are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SMP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SMP proteins, mutated SMP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SMP genes in this organism.
    Type: Grant
    Filed: June 23, 2000
    Date of Patent: September 18, 2007
    Assignee: BASF Aktiengesellschaft
    Inventors: Markus Pompejus, Burkhard Kröger, Hartwig Schröder, Oskar Zelder, Gregor Haberhauer
  • Process for the fermentative preparation of D-pantothenic acid and/or salts thereof
    Patent number: 7262034
    Abstract: The invention provides a process for the fermentative preparation of D-pantothenic acid and/or salts thereof or feedstuffs additives comprising these by fermentation of microorganisms of the Bacillus group, in particular those which already produce D-pantothenic acid, which comprises enhancing, in particular over-expressing, in the microorganisms one or more of the nucleotide sequence(s) which code(s) for the gene or ORF serA, serC, ywpJ or glyA.
    Type: Grant
    Filed: May 31, 2002
    Date of Patent: August 28, 2007
    Assignee: Degussa AG
    Inventors: Daniela Kruse, Georg Thierbach
  • Allele-specific mutation detection assay using an enzyme-disabling agent
    Patent number: 7262032
    Abstract: The present invention relates to a method of detecting a base at a pre-determined position in a nucleic acid molecule by performing enzymatic detection reactions using base-specific detection oligomers, where each oligomer is specific for a particular base at the predetermined position, and then comparing the enzymatic detection reactions to determine which base is present at the position, with an enzyme-disabling agent being present during the enzymatic detection reaction. In preferred embodiments the enzymatic detection reaction is an oligomer elongation extension reaction, catalysed by, among others, polymerase or ligase. Also disclosed are methods of performing the assay in a liquid phase and on microarrays.
    Type: Grant
    Filed: October 21, 2005
    Date of Patent: August 28, 2007
    Inventors: Afshin Ahmadian, Joakim Lundeberg
  • Assays for detecting modulators of cytoskeletal function
    Patent number: 7252950
    Abstract: Described herein are methods of identifying compounds which modulate the activity of the cytoskeletal system. The methods are rapid, convenient and sensitive. Preferably, the method is used to identify lead compounds that can be used as therapeutics, diagnostics and agricultural agents. Generally, test compounds are added to two cytoskeletal components which bind to one another, to determine whether the binding is affected by the test compound. Wherein the binding is affected, a compound which modulates the cytoskeletal system is identified.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: August 7, 2007
    Assignees: The Regents of the University of California, The Board of Trustees of The Leland Stanford Junior University
    Inventors: Ronald Vale, Daniel Pierce, James Spudich
  • Human pyruvate dehydrogenese phosphatase
    Patent number: 7250279
    Abstract: hPDP polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing hPDP polypeptides and polynucleotides in diagnostic assays.
    Type: Grant
    Filed: April 6, 2001
    Date of Patent: July 31, 2007
    Assignee: Merck Patent GmbH
    Inventors: Izaak Den Daas, Michael Gassen, Joachim Leibrock, Laurie Von Melchner
  • Method for producing proteins suitable for treating lysosomal storage disorders
    Patent number: 7241442
    Abstract: The present invention is based on the discovery that proteins produced in insect cell cultures are glycosylated in a unique manner that causes them to be selectively imported by cells that express mannose receptors on their membranes, particularly macrophages. Proteins that are selectively imported into cells containing mannose receptors are provided, as well as pharmaceutical compositions containing such proteins and methods for producing such proteins. Application of the present invention to produce proteins useful for treating lysosomal storage disorders is also disclosed. Engineering of cells to express mannose receptors so that they will selectively import proteins produced in insect cells is also taught, as well as a protein targeting system using such cells and proteins. Finally, an improved elution buffer for the purification of proteins produced in insect cells from a Concanavalin A column is provided.
    Type: Grant
    Filed: December 11, 2003
    Date of Patent: July 10, 2007
    Assignee: St. Jude Children's Research Hospital
    Inventors: Alessandra D'Azzo, Erik Jacobus Bonten
  • Phytase expression systems and methods of making and using them
    Patent number: 7232677
    Abstract: The invention provides a purified or recombinant phytase enzyme (SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
    Type: Grant
    Filed: February 5, 2001
    Date of Patent: June 19, 2007
    Assignee: Diversa Corporation
    Inventors: Jay M. Short, Keith A. Kretz
  • Production method and preparation method of glucans
    Patent number: 7229801
    Abstract: A first method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The maximum value of the sucrose-phosphate ratio of the reaction solution from the start of the reaction to the end of the reaction is no more than about 17. A second method for producing glucan comprises the step of allowing a reaction solution containing sucrose, a primer, inorganic phosphate or glucose-1-phosphate, sucrose phosphorylase, and glucan phosphorylase to react to produce glucans. The reaction is conducted at a temperature of about 40 C to about 70 C.
    Type: Grant
    Filed: May 27, 2002
    Date of Patent: June 12, 2007
    Assignee: Ezaki Glico, Co., Ltd.
    Inventors: Kazutoshi Fujii, Yoshinobu Terada, Michiyo Yanase, Koji Odan, Hiroki Takata, Takeshi Takaha, Takashi Kuriki, Shigetaka Okada
  • Sitosterolemia susceptibility gene (SSG) polypeptides
    Patent number: 7229816
    Abstract: The present invention provides polypeptides of a novel ABC family cholesterol transporter, SSG. The herein-disclosed sequences can be used for any of a number of purposes, including for the diagnosis and treatment of cholesterol-associated disorders, including sitosterolemia, and for the identification of molecules that associate with and/or modulate the activity of SSG.
    Type: Grant
    Filed: May 11, 2005
    Date of Patent: June 12, 2007
    Assignee: Amgen Inc.
    Inventors: Hui Tian, Joshua Schultz, Bei Shan
  • Triacylglycerol biosynthesis in the cytosol of eukaryotes
    Patent number: 7229815
    Abstract: This invention describes novel catalytically active cytosolic enzymes for triacylglycerol biosynthesis from eukaryotic systems. The complex from oleaginous yeast was enzymatically characterized, and was found to contain lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase, diacylglycerol acyltransferase, acyl—acyl carrier protein synthetase, superoxide dismutase and acyl carrier protein. The triacylglycerol biosynthetic machinery rapidly incorporates free fatty acids as well as fatty acyl-coenzyme A into triacylglycerol and its biosynthetic intermediates. Lysophosphatidic acid acyltransferase, phosphatidic acid phosphatase and diacylglycerol acyltransferase from the complex were microsequenced. Acyl carrier protein, superoxide dismutase and diacylglycerol acyltransferase genes were cloned and expressed in bacterial system.
    Type: Grant
    Filed: August 29, 2002
    Date of Patent: June 12, 2007
    Assignees: Indian Institute of Science, Bijam Biosciences Private Limited
    Inventor: Ram Rajasekharan
  • Assays and peptide substrate for determining aggrecan degrading metallo protease activity
    Patent number: 7223834
    Abstract: This invention is directed to assays to determine the presence or absence of proteins that exhibit aggrecanase or ADMP activity. This invention also relates to peptides that acts as a substrates for ADMPs, their use in various assays to determine the presence or absence of ADMP activity, and their use as inhibitors of ADMP activity.
    Type: Grant
    Filed: October 12, 2001
    Date of Patent: May 29, 2007
    Assignee: Bristol-Myers Squibb Pharma Company
    Inventors: Jeffrey A. Miller, Elizabeth C. Arner, Robert A. Copeland, Gary L. Davis, Ruiqin Liu, Michael A. Pratta, Micky D. Tortorella
  • Preparations for topical skin use and treatment
    Patent number: 7223386
    Abstract: Topical preparations for release of an active agent and to methods of making and using the topical preparations are provided. The preparations may have an internal phase dispersed within an external phase. The internal phase may be a hydrophilic carrier and an active agent. The external phase may be a silicone matrix.
    Type: Grant
    Filed: September 10, 2003
    Date of Patent: May 29, 2007
    Assignees: Dow Corning Corporation, Genencor International, Inc.
    Inventors: Richard R. Bott, Mark S. Gebert, Paal Christian Klykken, Isabelle Mazeaud, Xavier Jean-Paul Thomas
  • Peptidyl t-RNA hydrolase (PTH)
    Patent number: 7202071
    Abstract: The invention provides pth polypeptides and polynucleotides encoding pth polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pth polypeptides to screen for antibacterial compounds.
    Type: Grant
    Filed: May 14, 2003
    Date of Patent: April 10, 2007
    Assignee: SmithKline Beecham Corporation
    Inventors: Sanjoy Biswas, James Raymond Brown, Christine Debouck, Deborah Dee Jaworski, Elizabeth Jane Lawlor, Jeffrey Mooney, Lisa Kathleen Shilling, Min Wang, Richard Lloyd Warren, Magdalena Zalacain, Yi Yi Zhong, John Peter Throup, Karen Anne Ingraham, Alison Frances Chalker, David John Holmes, Christopher Michael Traini
  • Phosphatases which activate map kinase pathways
    Patent number: 7195902
    Abstract: The present invention provides novel JNK activating phosphatase polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, antibodies and methods for producing JNK activating phosphatase polypeptides. Also provided for are methods for the diagnosis and treatment of diseases associated with JNK activating phosphatase polypeptides.
    Type: Grant
    Filed: March 18, 2004
    Date of Patent: March 27, 2007
    Assignees: Amgen Corporation, Baylor College of Medicine
    Inventors: John W. Belmont, Frederick A. Fletcher, Alice J. Chen, Roland Jurecic, Tse-Hua Tan, Guisheng Zhou
  • Antibodies to a cathepsin C homolog
    Patent number: 7195926
    Abstract: The present invention provides nucleotide and amino acid sequences that identify and encode a new cathepsin C homolog (RCP) expressed in THP-1 cells. The present invention also provides for antisense molecules to the nucleotide sequences which encode RCP, expression vectors for the production of purified RCP, antibodies capable of binding specifically to RCP, hybridization probes or oligonucleotides for the detection of RCP-encoding nucleotide sequences, genetically engineered host cells for the expression of RCP, diagnostic tests for activation of monocyte/macrophages based on RCP-encoding nucleic acid molecules, and use of the protein to produce antibodies capable of binding specifically to the protein and use of the protein to screen for inhibitors.
    Type: Grant
    Filed: March 8, 2000
    Date of Patent: March 27, 2007
    Assignee: Incyte Corporation
    Inventors: Roger Coleman, Scott Michael Braxton, Jeffrey J. Seilhamer
  • Method for the production of -hydroxybenzoate in species of pseudomonas and
    Patent number: 7195898
    Abstract: Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.
    Type: Grant
    Filed: July 11, 2006
    Date of Patent: March 27, 2007
    Assignee: North Carolina State University
    Inventors: Arie Ben-Bassat, Monica Cattermole, Anthony A. Gatenby, Katharine J. Gibson, M. Isabel Ramos-Gonzalez, Juan Ramos, Sima Sariaslani
  • Bandage composition containing phage associated lytic enzymes useful for treating dermatological infections
    Patent number: 7169408
    Abstract: The present invention discloses a composition for dermatological infections by the use of a lytic enzyme in a carrier suitable for topical application to dermal tissues. The method for the treatment of dermatological infections comprises administering a composition comprising effective amount of a therapeutic agent, with the therapeutic agent comprising a lytic enzyme produced by infecting a bacteria with phage specific for that bacteria.
    Type: Grant
    Filed: June 20, 2003
    Date of Patent: January 30, 2007
    Assignees: New Horizons Diagnostics Corp., Rockefeller University
    Inventors: Vincent Fischetti, Lawrence Loomis
  • Rat secreted embryonic alkaline phosphatase
    Patent number: 7157261
    Abstract: Rat secreted embryonic alkaline phosphatase and methods for it use are provided.
    Type: Grant
    Filed: October 18, 2004
    Date of Patent: January 2, 2007
    Assignee: Canji, Inc.
    Inventor: Timothy P. Mayall
  • Use of bacterial phage associated lysing enzymes for treating upper respiratory illnesses
    Patent number: 7141241
    Abstract: The present invention relates to an oral delivery system containing a bacteriophage associated lysin enzyme for the treatment of [Streptococcus pneumoniae and] Hemophilus influenzae sinus/nasal infections.
    Type: Grant
    Filed: February 8, 2002
    Date of Patent: November 28, 2006
    Assignees: New Horizons Diagnostics Corp, Rockefeller University
    Inventors: Vincent Fischetti, Lawrence Loomis
  • having accession No. PTA 1579 and its use to produce polyhydroxybutyrate
    Patent number: 7135316
    Abstract: The present invention provides a novel genetically modified Escherichia coli JM109 bearing accession number PTA 1579, containing the gene coding for poly-beta-hydroxybutyrate synthesis and a method of using this bacterium to produce poly-beta-hydroxybutyrate to the extent of 60% or more of the cell weight.
    Type: Grant
    Filed: November 19, 2003
    Date of Patent: November 14, 2006
    Assignee: Council of Scientific & Industrial Research
    Inventors: L. H. Mahishi, G. Tripathi, T. V. N. Ramchander, Shuban Kishen Rawal
  • Expression of lysosomal hydrolase in cells expressing pro-N-acetylglucosamine-1-phosphodiester ?-N-acetyl glucosimanidase
    Patent number: 7135322
    Abstract: The present invention provides methods of producing a pro-N-acetylglucosamine-1-phosphodiester?N-acetyl glucosimanidase (phosphodiester ?-GlcNAcase), in mammalian cells deficient in the furin proteolytic enzyme and methods of making lysosomal bydrolases having an N-acetylglucosamine-1-phosphate.
    Type: Grant
    Filed: July 29, 2004
    Date of Patent: November 14, 2006
    Assignee: Novazyme Pharmaceuticals, Inc.
    Inventors: William Canfield, Stuart Kornfeld
  • Nucleoside triphosphate diphosphohydrolase (NTPDase 8) and uses thereof
    Patent number: 7129074
    Abstract: The present invention relates to a new ectohydroxynucleotidase, namely the NTPDasse8, which allows to regulate platelet aggregation or activation involved in the formation of thrombosis and related diseases. The nucleic acid sequence and the corresponding amino acid sequence and uses thereof are described.
    Type: Grant
    Filed: March 11, 2005
    Date of Patent: October 31, 2006
    Assignee: Université Laval
    Inventor: Jean Sevigny
  • Human mater proteins
    Patent number: 7125684
    Abstract: Two human MATER proteins as well as their use for fertility disorders, therapy and diagnosis are described.
    Type: Grant
    Filed: August 12, 2002
    Date of Patent: October 24, 2006
    Assignee: Schering AG
    Inventors: Bertram Weiss, Monika Lessl, Michaele Peters-Kottig, Georg Beckmann
  • Directed evolution of microorganisms
    Patent number: 7125695
    Abstract: The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
    Type: Grant
    Filed: November 20, 2003
    Date of Patent: October 24, 2006
    Assignee: Genencor International, Inc.
    Inventors: Volker Schellenberger, Amy D. Liu, Olga V. Selifonova
  • Kit for promoting the growth of human hair and its method of use
    Patent number: 7125544
    Abstract: Provided is a kit that composes of two products and its method of use to promote hair growth. The kit has two products that are applied to the scalp of a human sequentially. The first product applied to the scalp of a human is a monoethanolamine salt composition that is combined with an organic enzyme. The second product is an oxidizing agent.
    Type: Grant
    Filed: March 29, 2005
    Date of Patent: October 24, 2006
    Inventor: Amparo Arango
  • Phosphodiesterases
    Patent number: 7122362
    Abstract: The invention provides human phosphodiesterases (HPDE) and polynucleotides which identify and encode HPDE. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of HPDE.
    Type: Grant
    Filed: January 26, 2001
    Date of Patent: October 17, 2006
    Inventors: Mariah R. Baughn, Dyung Aina M. Lu, Henry Yue, Y. Tom Tang, Danniel B. Nguyen
  • N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor
    Patent number: 7119188
    Abstract: This invention relates to isolated or recombinant N-carbobenzyloxy-deprotecting enzyme polypeptides, and variants and fragments thereof, that catalyze the removal of carbobenzyloxy from carbobenzyloxy-protected amino acids and alcohols. Also related are isolated nucleic acids encoding N-carbobenzyloxy-deprotecting enzyme polypeptides, and variants, and fragments thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using the polypeptides in various reactions for industrial or pharmaceutical applications.
    Type: Grant
    Filed: June 3, 2003
    Date of Patent: October 10, 2006
    Assignee: Bristol-Myers Squibb Company
    Inventors: Venkata B. Nanduri, Ramesh N. Patel, Steven L. Goldberg, Robert M. Johnston
  • Factors participating in degranulation of mast cells, DNAs encoding the same, method of screening of these factors and the inhibitors
    Patent number: 7108987
    Abstract: The present invention provides a novel protein that regulates degranulation of mast cells (degranulation regulator), a gene encoding it, a protein (conjugate factor) that interacts with the regulator, a gene encoding it, a screening method of an inhibitor of the degranulation, which uses this degranulation regulator and the conjugate factor, an inhibitor obtained by the screening method and the like.
    Type: Grant
    Filed: April 16, 2001
    Date of Patent: September 19, 2006
    Assignee: Dainippon Pharmaceutical Co., Ltd.
    Inventors: Tsuyoshi Yamada, Motoharu Ido
  • Recombinant lysophosphatidic acid phosphatase
    Patent number: 7109012
    Abstract: An object of the present invention is to provide a recombinant LPA phosphatase capable of specifically hydrolyzing LPA, which is useful for elucidation of the metabolism pathway of LPA, and also for diagnosis and treatment of various diseases with which LPA is associated; a method capable of simply and inexpensively determining LPA associated with various diseases; and a kit for determination suitable for the method. The present invention has succeeded in purifying the LPA phosphatase using bovine brain as raw material, and further in cloning human LPA phosphatase gene.
    Type: Grant
    Filed: August 7, 2002
    Date of Patent: September 19, 2006
    Assignee: Azwell Inc.
    Inventors: Tadaomi Takenawa, Masami Hiroyama, Tatsuya Kishimoto, Masahiro Yamaguchi, Mitsuyoshi Toyosato, Kouji Mizuno
  • ahl-acylase gene
    Patent number: 7098014
    Abstract: This invention provides a gene, qsbA, which encodes a protein useful for inactivating certain bacterial quorum-sensing signal molecules (N-acyl homoserine lactones) which participate in bacterial virulence and biofilm differentiation pathways. This gene was isolated from Ralstonia sp., strain XJ12B. The invention also provides the QsbA protein, which possesses N-acyl homoserine lactone inactivating activity.
    Type: Grant
    Filed: January 23, 2002
    Date of Patent: August 29, 2006
    Inventors: Lian Hui Zhang, Jin Ling Xu, Yi Han Lin
  • Isolation and composition of novel glycosidases
    Patent number: 7094563
    Abstract: Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac?1-x, Gal?1-3R, Gal?1-6R, Gal?1-3R, Fuc?-2R, Fuc?1-3R, Fuc?1-4R, Man?1-2R, Man?1-3R, Man?1-6R, Man?1-4R, Xyl?1-2R, Glc?1-4R, and Gal?1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.
    Type: Grant
    Filed: November 15, 2001
    Date of Patent: August 22, 2006
    Assignee: New England Biolabs, Inc.
    Inventors: Sharon T. Wong-Madden, Ellen P. Guthrie, David Landry, Christopher H. Taron, Chudi Guan, Phillips W. Robbins
  • Consensus phytases
    Patent number: RE39649
    Abstract: A process for obtaining a consensus protein from a group of amino acid sequences of a defined protein family, proteins and polynucleotides so obtained, and compositions containing such proteins.
    Type: Grant
    Filed: May 24, 2005
    Date of Patent: May 22, 2007
    Assignee: DSM IP Assets B.V.
    Inventor: Martin Lehmann