Abstract: The present invention provides for the production of ASA from yeast capable of producing ASA from KLG. The present invention provides methods for the production of ASA as well as recombinant yeast capable of producing ASA from a carbon source.

Abstract: It is an object to provide an organic waste processing method and apparatus capable of making a formalin nontoxic and decreasing an amount and/or a volume of a formalin-fixed organic waste easily in a shorter time without retaining an original form.

Abstract: A method of rapid biochemical cycling of aquariums using naturally preserved granular marine substrate material, such as sand or aragonite, to rapidly denitrify the aquatic environment and to establish biochemical conditions that are favorable to the survival and viability of fish, crustaceans, invertebrates, and other marine aquatic life. The method includes the steps of harvesting and packaging marine sand such that marine microorganisms, in the form of biofilm attached to the sand, are preserved with an optimal amount of water and air in retail packaging specifically dimensioned and configured for maintaining ammonia oxidizing bacteria in a state wherein the bacteria are capable of metabolic and physiologic activity after prolonged periods at room temperature. Harvesting and packaging marine microorganisms according to the disclosed method allows for widespread distribution to consumers through conventional retail sales channels.

Abstract: A three-stage method used for producing biogas having a high methane content from organic substances includes aerobic fermentation, a charring and thermophilic methane fermentation.

Abstract: A method of biodegrading municipal solid waste includes providing a quantity of municipal solid waste and promoting anaerobic digestion in the quantity of municipal solid waste by methanogenic organisms to produce methane. The methane production is monitored and when it is determined that production of methane from the quantity of municipal solid waste has subsided, aerobic digestion of the quantity of municipal solid waste is promoted. A gas collection system in operative association with the quantity of organic waste collects biogas and the biogas is monitored and the rate of gas collection is adjusted as necessary to maximize methane collection. A leachate collection system collects leachate from the quantity of municipal solid waste and circulates the leachate back to the quantity of organic waste during the anaerobic digestion and aerobic digestion steps.

Abstract: The present invention relates to process control where some of the controllable parameters are difficult or impossible to characterize. The present invention relates to process control in biotechnology of such systems, but not limited to. Additionally, the present invention relates to process control in biotechnology minerals processing. In the inventive method, an application of the present invention manipulates a minerals bioprocess to find local exterma (maxima or minima) for selected output variables/process goals by using a learning-based controller for bioprocess oxidation of minerals during hydrometallurgical processing. The learning-based controller operates with or without human supervision and works to find processor optima without previously defined optima due to the non-characterized nature of the process being manipulated.

Abstract: The invention relates to a method and device for accelerating the decomposition of biogenic organic matter in refuse disposal sites. According to the method, at least one suction line is inserted into a disposal site debris heap (11) via which the arising refuse site gases are removed by suction. According to the invention, the quantity of gas removed by suction in the area of the bottom of the refuse disposal site should be greater than the quantity of gas arising in this area due to decomposition whereby enabling oxygen from the outside air to penetrate into the debris heap and transforming the decomposition process into an, at least to a large extent, aerobic decomposition.

Abstract: A method of removing pollutants from porous, solid materials uses a biomass loaded onto a support. The biomass is put into contact with a pollutant contaminated porous, solid material so that the bacterial biomass degrades the pollutant. The moisture level of the support and biomass are maintained at a level that optimizes pollutant removal and is a function of the relative solubility of the pollutant.

Abstract: A method is described for the decomposition of oil in which one applies to oil a composition made of bacteria-containing, dealginated, partially dewatered kelp residue. The composition will have a water content no greater than 25%, preferably about 5%-10%. The composition material is maintained in contact with the oil with sufficient water to sustain the bacteria and for a period of time sufficient for the oil to be decomposed by the bacteria.

Abstract: The present invention is directed to a method and system of system for efficiently bio-converting putrescent wastes to a more usable form. The present “domestic” unit does not utilize a motor nor does in contend any moving parts. Instead, the domestic unit generally comprises a generally rounded container with two small ramps on the inside of the container. The two ramps begin at the bottom of the container and spiral up to the top of the container, where they adjoin a discharge pipe. In operation, the putrescent waste is deposited into the domestic unit container. Mature larvae have only one avenue of escape from the putrescent waste, up the ramps and into discharge pipe and onto collection tubes where the larvae are collected and processed. When the container fills up with larval residue, the larvae are removed from the container, the container is emptied of residue, and the larvae are put back into the container.

Abstract: Described are new micro-organisms and a new enzyme capable of using as sole source of nitrogen the propionic acid amide of formula (VI), in racemate form or as optically active isomers. Described also is a method of preparing (S)- or (R)-3,3,2-trifluoro-2-hydroxy-2-methylpropionic acid of formulas (I) and (II) starting from trifluoroaceto-acetic ester. The first three process steps are chemical, the fourth process step microbiological.

Abstract: The present invention describes a wastewater treatment method by a microorganism decomposing Tetramethyl Ammonium Hydroxide (TMAH) which, utilized in etching the surface of silicone chip in semiconductor manufacturing process, is toxic and hard to decompose. The present invention provides novel microorganisms capable of decomposing TMAH. Also, the present invention provides a treatment method for wastewater containing TMAH, using the microorganisms. The present invention is useful in industrial field as an environmental friendly wastewater treatment method by decomposing over 90% of TMAH, one of environmental contamination materials in the wastewater of semiconductor factory.

Abstract: The invention relates to an effective and environmentally safe process for the microbial leaching of sulfidic materials, particularly of sulfide ores such as pyrite, marcasite, chalcopyrite, bornite, or covelline, which process is characterized in that the aqueous leaching fluid is added with sulfur-containing amino acids or derivatives thereof. The invention is also directed to the use of sulfur-containing amino acids or derivatives thereof in the microbial leaching of sulfidic materials, particularly in pyrite leaching.

Abstract: A method of accelerated remediation or bioremediation of contaminated material such as manure-contaminated material is provided comprising generating a treated contaminated material entraining air stream at a velocity sufficient for entraining the contaminated material therein. The contaminated material is entrained in the air stream and is then microenfractionated to form a microenfractionated contaminated material. Finally, the microenfractionated contaminated material is treated with a least one chemical amendment and/or one biological amendment thereby facilitating the accelerated remediation or bioremediation. The chemical amendment can comprise either a chemical oxidizing agent, a chelating agent, or a metallic reducing agent. The preferred metallic reducing agents are zero valent iron, zero valent zinc, zero valent tin, zero valent manganese and zero valent aluminum.

Abstract: A composition useful for cleaning and bioremediation applications is provided which comprises a Pseudomonas species, an alkali metal nitrate, an ethoxylate nonionic surfactant, and optionally monoammonium phosphate as a buffer in an aqueous solution. The Pseudomonas sp. is stable during storage of the composition.

Abstract: The present invention provides a method of biological treatment for purifying an environmental pollutant utilizing microorganisms, wherein the pollutant is efficiently concentrated to a high concentration but exerts substantially no effect on the biological activity of the microorganisms used to biodegrade the pollutant, so that the pollutant are purified (assimilated or degraded) effectively. The present invention also provides a microbial treatment agent useful for the purification treatment of an environmental pollutant. Specifically stated, the method for purification treatment of the present invention can be carried out by making an environmental pollutant and microorganisms to coexist with each other as incorporated in a microorganism-produced polymer. The microbial treatment agent of the present invention comprises microorganisms incorporated in a microorganism-produced polymer.

Abstract: A process for treating waste comprising the steps of (i) adding a first innoculum comprising sulfide-utilizing bacteria to a sulfide-rich organic waste to produce a lowered-sulfide waste, (ii) adding a second innoculum comprising organic digesting bacteria to the lowered-sulfide waste to produce a digested waste, and (iii) adding to the digested waste at least one of (a) a third innoculum comprising bacteria capable of converting a nitrogen-containing compound to N2 to produce a lowered nitrogen waste, and (b) a fourth innoculum comprising phosphorous absorbing bacteria to produce a phosphorous enriched bacteria fraction and a phosphorous depleted waste fraction.

Abstract: A method has been developed to monitor the health of an activated sludge environment in a wastewater process comprising monitoring the levels of polyhydroxyalkanoates (PHA) produced and correlating those levels with various selected sample parameters. In general, levels of PHA in excess of about 15% to about 20% dry weight of the biomass is an indication that the biocatalytic efficiency of the wastewater treatment process is impaired.

Abstract: A porous container for controllably releasing various substances into surrounding fluid environments includes a porous material having pores sized and configured to allow the substances contained within the container to pass through the pores at a rate of no more than about 0.5 g/day/cm2. In a particular embodiment, the porous container is adapted to controllably release microorganisms into surrounding fluid environments, which microorganisms may be cultured within the porous container. The porous container may also be utilized with a variety of dispensing vessels to obtain desired controlled release characteristics.

Abstract: A process for regenerating an adsorbent having adsorbed hardly decomposable substances. The process comprises decomposing the hardly decomposable substances by bringing the substances into contact with a microorganisms and/or an enzyme at an inside or a peripheral portion of pores of the adsorbent. The adsorbent can be regenerated by decomposing and removing the adsorbed substances which are hardly decomposable and harmful to living organisms, such as bisphenols, safely from the adsorbent without adverse effects on the environment such as the secondary pollution.

Abstract: A disposal method and disposal system for sharply reducing the residue and enhancing the sustainability and stability of disposal in disposal of organic waste are provided. The matter inside the solid phase decomposer is transported to a liquid phase decomposer I and is decomposed by land microorganisms at a solid phase decomposer III. The highly viscous products built up at that time are transported to the liquid phase decomposer I where they are dissolved in water and converted to sludge by aqueous microorganisms. The newly introduced organic waste is washed at the liquid phase decomposer I, transported together with the produced sludge to the solid phase decomposer III, and again decomposed by land microorganisms. For the transport of the matter inside the solid phase decomposer to the liquid phase decomposer I and the transport of the organic waste to the solid phase decomposer III, a solid/liquid circulator II having a twin screw structure is used.

Abstract: Liquid chemical compositions are disclosed for anaerobic biodegradation, detoxification, and transformation of toxic organic and inorganic compounds in a contaminated geologic media under reducing conditions, including, but not limited to, denitrifying, manganese-reducing, iron-reducing and sulfate-reducing conditions. One such liquid chemical composition includes sodium nitrate in the range of one-fifth (0.2) to four (4) pounds per gallon of the chemical composition; sodium hexametaphosphate or other biologically hydrolyzable ring or linear polyphosphate in the range of one twentieth (0.05) to five (5) pounds per gallon of the chemical composition; a surfactant in the range of 0.01% to 10% by volume of the chemical composition; and a diluent in the form of water.

Abstract: A process for decreasing the reducing sugars content of light steepwater comprising complementary incubation of light steepwater from a corn steeping process having sulfur dioxide (SO2) content at between about 20 ppm and about 220 ppm for at least about 5 hours. The process is particularly useful for obtaining Pharmaceutical Industry Grade Steepwater.

Abstract: Method and apparatus for biologically oxidizing organic matter. Finely powdered organic matter is suplied into the top end of an upright, high reaction vessel (1). The vessel (1) has been filled up substantially completely by small balls, on the surfaces of which the matter to be oxidized is present in thin layers. By means of a conveyor (6) outside the vessel, the balls are transferred uppwards, whereby the balls in the reaction vessel (1) flow downwrds. Oxidizing air is blown uppwrds against the flow of balls, whereby in the upper section of the vessel, drying takes place in the first instance and then is formed an oxidizing zone automatically by microbial activity in a section, wherein the best oxidizing conditions are prevailing. Exhaust air is cleaned by means of ionic cleaners (14) and singlet oxygen generators (27) and oxidized matter is recovered from the bottom end of the reaction vessel.

Abstract: A process for treating a mixture of substances containing structured constituents and organic matter, and a device for carrying out this process are disclosed. In accordance with the invention, the mixture of substances is subjected to pulse-type or periodical application of force, so that the formation of flow channels for a leaching fluid or process air in a bulk material may be prevented.

Abstract: The present invention discloses the formulation and use of an advanced solid-media chemical composition which includes both plant-derived and inorganic components which is designed and intended to enhance the removal of a broad range of recalcitrant organic and inorganic contaminants in the environment by providing an improved means of promoting the anaerobic, biologically mediated degradation, transformation, and/or detoxification of the contaminants which may be present in solid and liquid wastes, soils, sediments, and water bodies. The invention provides for improved means of (i) promoting the solid-phase extraction, absorption, and adsorbtion of recalcitrant contaminants from contaminated media, (ii) creating, enhancing, and maintaining anaerobic and highly reducing conditions (i.e.

Abstract: Disclosed is a particulate of an ore material having a metal value metal value and a matrix material having a sulfur content wherein the sulfur is present in an oxidation-reduction state of zero or less, the particulate comprising (a) a core particle of the ore material, with the core particle having a size of less than about two inches; and (b) a layer of particles of the ore material and an inoculate of sulfur biooxidizing bacteria in admixture with the particles; wherein the particulate has sufficient strength to retain at least about 95% of a void volume for a column height of about 6 feet for at least 200 days when continuously bathed in a solution comprised of the inoculate or a nutrient therefor. Also disclosed is a heap comprising a plurality of the particulate.

Abstract: A method and an apparatus is provided for aeration of organic material, wherein the organic material undergoes an aerobic composting process, such that air is caused to evenly penetrate the organic material. The organic material is first sealed in a vessel. Air is then delivered to the contents of the vessel such that an air pressure of from about 1 to about 1000 kPa above atmospheric pressure is maintained in the vessel. A pressure differential initially forms and is then allowed to equilibrate over a period of time, in which time air flows from areas of high pressure to areas of low pressure in the organic material thus ensuring that aeration is evenly distributed throughout the organic material.

Abstract: The present invention provides a method and gaseous composition for the in situ bioremediation of soil and groundwater contaminated with organic compounds, including halogenated hydrocarbons. The gaseous composition, which readily permeates a subsurface region, comprises hydrogen (H2) and one or more volatile phosphates, such as triethylphosphate (TEP) and tributylphosphate (TBP). The volatile phosphates serve as nutrients that stimulate the growth and activity of indigenous microbes that are capable of degrading the contaminants. The addition of hydrogen facilitates the direct reductive dehalogenation of highly halogenated contaminants. The gaseous composition may optionally contain one or more of a volatile alkane and nitrous oxide as additional supports for microbial growth, and carbon dioxide to lower the pH of remediation sites that are highly alkaline.

Abstract: A method for degrading and/or bioremediating waste wood containing creosote using a fungal inoculum is disclosed. The fungal inoculum comprises of at least one creosote-tolerant fungi, a lignocellulosic substrate and a nutrient supplement. The fungal inoculum is applied to the waste wood and maintained in an aerated and hydrated environment having temperature conditions sufficient to allow the inoculum to grow and metabolize the creosote. The inoculum and the waste wood are combined until an end product is achieved that is at least partially remediated or of a reduced volume.

Abstract: The invention relates to methods of solubilizing and recycling polymers using irradiation, wherein the polymers comprise photoreactive moieties. These polymers have many applications including use in disposable consumer products such as beverage bottles, eating utensils and diapers.

Abstract: Solutions containing microorganisms differing in characteristics from each other and living in symbiosis with each other and enzymes characterized by containing aerobic microorganisms, anaerobic microorganisms and at least one basidiomycete belonging to the family Pleurotaceae living in symbiosis, metabolites thereof and enzymes; carriers obtained by adsorbing the components of the above solutions onto finely ground carbonaceous materials; and porous materials obtained by adsorbing the components of the above solutions onto porous materials. Because of having various effects of absorbing, adsorbing and decomposing harmful matters, deodorizing, decolorizing, etc., these materials are applicable to various uses in the fields of agriculture and environment.

Abstract: A method of remediating an environment contaminated with carbon tetrachloride by introducing Pseudomonas strain sp. KC (PsKC) into the environment under iron limiting conditions and converting the carbon tetrachloride directly to carbon dioxide and a non-volatile water soluble fraction. Further, conditions providing a niche advantage for PsKC in accordance with the invention allow for the use of PsKC as a vector in various other environmental conditions for introducing specific activities of the PsKC, whether naturally occurring or genetically altered, into those environments.

Abstract: A method for obtaining oil products such as diesel fuel with improved quality, from a gas oil fraction containing organosulphur compounds such as dibenzenethiophenes and/or their derivatives, with initial and final boiling points generally ranging between about 170 and 480° C. The method includes at least two steps which consist in: (a) liquid-liquid extraction wherein the diesel fuel fraction is contacted with a solvent so as to obtain a gas oil-type raffinate with low content of sulphur and aromatic compounds and an extract rich in solvent and with high content of sulphur and aromatic compounds; (b) oxidation of the extract sulphur compounds so as to obtain, after separation, a heavy gas oil-type hydrocarbon effluent with low sulphur content, and a residue comprising oxidased organosulphur compounds. The invention also concerns oil products obtained by said method which can be used either for the formation of fuel for internal combustion engine with compression ignition (of the diesel type) or as fuel.

Abstract: A gypsum-based building material comprising gypsum as the main material and an anthraquinone compound incorporated.

Abstract: Hydrophobic compositions and methods for using hydrophobic compositions in improving the water quality of water applications. The hydrophobic composition comprising hydrophobic carriers and microbial agents and wherein the hydrophobic composition distributes the microbial agent efficiently and uniformly to the water application and target plants and/or organisms.

Abstract: The present invention relates to apparatus, methods, and applications for treating wastewater, and more particularly to biological processes for removing pollutants from wastewater.

Abstract: The present invention relates to xyloglucanases belonging to family 5 of glycosyl hydrolases are derived from strains of Paenibacillus, especially from strains of Paenibacillus pabuli. The xyloglucanases show high performance in conventional liquid detergent compositions.

Abstract: A bioremediation system converts a waste stream, at least part of which is a fluid containing energetics, to carbon dioxide (CO2), water, and environmentally benign end products. It uses gas-enhanced sequencing-batch-reactors (SBRs), treating the waste stream in three SBRs seriatim. The first SBR uses a nitrogen purge, the second a hydrogen gas supplement, and the third an oxygen gas or forced air supplement. Each reactor may be supplemented with additives to optimize conditions such as pH, dissolved oxygen, and nutrient level. The system may be implemented under manual control, semi-automated, or fully automated, as needed. A waste stream of consideration is the pink water resultant from munitions fabrication and handling.

Abstract: The present invention relates to isolated polypeptides having glucanotransferase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: A system is disclosed for reducing costs associated with making a plurality of food products incompatible in a single food product-making facility by a plurality of food product makers. A plurality of discrete food product-making plants are provided configured for use by the plurality of food product makers. A common area facility includes facilities sharable by the plurality of food product makers using and occupying the plurality of food product making plants. The plurality of food product making plants and common area facility are operated cooperatively by sharing use and costs associated with use of the common area facility by and among the plurality of food product makers.

Abstract: An enzyme isolated from an organism that metabolizes alpha-galactosyl containing saccharides, comprising an alpha-galactosidase (E.C. 3.2.1.22, alpha-D-galactoside galatohydrolase) with optimal activity in the neutral to alkaline pH range, and which hydrolyzes a variety of alpha-galactose containing saccharides, in particular raffinose. The enzyme is preferably a protein monomer and an ex-alpha-galactosidase.

Abstract: The present invention relates to an enzymatic composition capable of killing or inhibiting microbial cells or micro-organisms, e.g. in laundry, on hard surfaces, in water systems, on skin, on teeth or on mucous membranes. The present invention also relates to the use of said enzymatic composition for preserving food products, cosmetics, paints, coatings, etc.

Abstract: The present invention provide: a bioreactor carrier having a suitable water swelling property, a suitable degree of volumetric swelling and a suitable specific gravity as well as exhibiting high chemical resistance to be suitable for use in the microbial treatment of organic waste water having a high chemical content; processes for efficiently producing the bioreactor carrier; and use of the bioreactor carrier. More specifically, there are provided: a chemical-resistant bioreactor carrier comprising (A) a specific water-swellable thermoplastic resin and (B) a specific resin compatible with the water-swellable thermoplastic resin (A), and as the case may be, (C) an inorganic filler, characterized in that the degree of volumetric swelling of the carrier is controlled to within a range of 120 to 3,000% and that the specific gravity of the carrier on swelling in water is controlled to within a range of 0.98 to 2.

Abstract: The use of fatty acid oxidizing enzymes in the manufacture of paper materials, such as paper, linerboard, corrugated paperboard, tissue, towels, corrugated containers and boxes. Examples of fatty acid oxidizing enzymes are oxygenases classified as EC 1.13.11. including any of the sub-classes thereof, such as lipoxygenase, EC 1.13.11.12. The effect of these enzymes is that the deposition of pitch is reduced, and bleaching and de-inking effects are also observed on the paper pulp and the resulting paper material. The fatty acid oxidizing enzyme can be used in combination with a substrate, with proteases, lipases, xylanases, cutinases, oxidoreductases, cellulases, endoglucanases amylases, mannanases, steryl esterases, and/or cholesterol esterases; or with surfactants and other adjuvants.

Abstract: The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development.

Abstract: A composition and method for reducing the levels of pathogenic bacteria in an aquatic environment. The composition includes isolated Bacillus cereus which are able to reduce a number of well known pathogenic bacterium from aquatic environments.

Abstract: The present invention is directed to a novel method, composition and device for removing dissolved oxygen from solutions containing alcohols and/or acids. By removing oxygen from various products, the present invention is an effective antioxidant for beverages and food products, as well as for industrial and commercial solutions containing alcohols and/or acids.

Abstract: A method is described for the decomposition of oil in which one applies to oil a composition made of bacteria-containing, dealginated, partially dewatered kelp residue. The composition will have a water content no greater than 25%, preferably about 5%-10%. The composition material is maintained in contact with the oil with sufficient water to sustain the bacteria and for a period of time sufficient for the oil to be decomposed by the bacteria.

Abstract: The subject invention provides materials and methods for the control of pests. Specifically exemplified is the use of recombinant hosts to control mosquito larvae. These hosts, which may be, for example yeast or algae, can be transformed so that they express a pesticidal polypeptide which controls mosquito larvae. These transformed microbes can then be applied to surface waters where mosquito larvae are likely to be found.