Abstract: A wetness indicator material may be used on a substrate to form a wetness sensor. The sensor may show either the presence or absence of an aqueous-based fluid or water-containing medium, such as vaginal fluid or urine in a personal hygiene article. The wetness sensor may be incorporated into the article. The wetness indicator material includes a standard colorant that does not change color when wetted. The standard colorant increases the range of hues exhibited by the wetness indicator material.
Type:
Grant
Filed:
September 12, 2011
Date of Patent:
December 16, 2014
Assignee:
Kimberly-Clark Worldwide, Inc.
Inventors:
Xuedong Song, Karen Meloy Goeders, Ning Wei
Abstract: This application provides devices for modeling ischemic stroke conditions. The devices can be used to culture neurons and to subject a first population of the neurons to low-oxygen conditions and a second population of neurons to normoxic conditions. The neurons are cultured on a porous barrier, and on the other side of the barrier run one or more fluid-filled channels. By flowing fluid with different oxygen levels through the channels, one can deliver desired oxygen concentrations to the cells nearest those channels.
Type:
Grant
Filed:
February 4, 2013
Date of Patent:
December 16, 2014
Assignees:
The Charles Stark Draper Laboratory, Inc., The University of South Florida
Inventors:
Anilkumar Harapanahalli Achyuta, Javier Cuevas, Shivshankar Sundaram, Chris Katnik
Abstract: An LED-based materials analysis apparatus that measures the photoelectrochemical response of materials to illumination. The apparatus uses an array of light sources such as a plurality of LEDs that provide light of desired wavelengths to illuminate one or more samples of materials of interest that are immersed in an electrolyte. A measurement circuit is connected between a transparent conductor attached to each sample of interest and a counter electrode. In some measurements, a third, standard electrode may be connected to the measurement circuit. A pulsing circuit that operates the LEDs causes each sample to be tested according to a predetermined sequence. Data is collected using a programmable computer operating under the control of instructions recorded on a machine readable medium. The data is analyzed and is available to be displayed to a user, recorded in a database, or communicated to another apparatus or process.
Abstract: An automated instrument for identification and/or characterization of a microbial agent present in a sample. The instrument includes (a) a sample removal apparatus operative to remove a test sample from a specimen container and add the test sample to a disposable separation device; (b) a separation and concentration apparatus operative on the separation device to separate the microbial agent from other components which may be present in the test sample and concentrate the microbial agent within the separation device; and (c) a identification and/or characterization module interrogating the concentrated microbial agent to identify and/or characterize the microbial agent.
Type:
Grant
Filed:
May 14, 2010
Date of Patent:
December 16, 2014
Assignee:
Biomerieux, Inc
Inventors:
Ronnie J. Robinson, Mark S. Wilson, Christopher S. Ronsick, John D. Walsh, Jones M. Hyman, Bradford G. Clay
Abstract: One feature pertains to a radiation dosimeter comprising a microdosimeter cell array that includes a first microdosimeter cell having a first semiconductor volume configured to generate a first current in response to incident radiation. The first semiconductor volume may have at least one of a first size, a first shape, a first semiconductor type, and/or a first semiconductor doping type and concentration that is associated with a first biological cell type or a first biological cell component type. The dosimeter may further comprise a processing circuit communicatively coupled to the microdosimeter cell array and configured to generate a signal based on the first current. The signal generated may be indicative of an amount of radiation absorbed by the microdosimeter cell array. A display may be utilized by the dosimeter to show a radiation level reading based on the signal generated.
Type:
Application
Filed:
August 26, 2014
Publication date:
December 11, 2014
Inventors:
James Francis Ziegler, Chao-tuan Liu, Razmig Hagop Messerian, Wayne Newhauser
Abstract: A single injection gradient with a biosensor, both structural and methodological, achieves the binding of analyte to immobilized ligand over a wide concentration range without the necessity of regeneration of the sensing area. A gradient of concentrations adjacent to or within a flow cell facilitates kinetic analysis of interactions without requiring multiple discrete volumes or injections to achieve a range of concentrations. A continuous gradient fluid is preferably formed directly adjacent to the flow cell inlet or a region of sample/buffer dispersion at an injection point into a flow channel of a flow cell. The analyte gradient may be flowed through the flow cell from a low analyte concentration. Multiple component gradients are also provided.
Abstract: A microbial detection article and methods of using the same, the article comprising: a base member comprising a self-supporting water impervious substrate with first and second generally opposed major surfaces; a filter assembly defining a filter assembly aperture therein, and having a composite filter body mounted across the filter assembly aperture; wherein the composite filter body comprises: a microporous membrane, and a water-absorbent layer in fluid communication with the microporous membrane; and a cover sheet.
Type:
Grant
Filed:
December 27, 2011
Date of Patent:
December 9, 2014
Assignee:
3M Innovative Properties Company
Inventors:
Steven P. Swanson, Jesse D. Miller, Clinton P. Waller, Jr., Neil Percy, Jeffrey A. Lucas, Kannan Seshadri, Jerald K. Rasmussen, Douglas E. Weiss, James E. Aysta
Abstract: A chilled reagent container comprises a reagent vessel containing part for containing therein a plurality of reagent vessels, a container lid including a container lid hole through which the reagent vessels contained by the reagent vessel containing part are accessible, and a cooling block for cooling the reagent vessels contained by the reagent vessel containing part, wherein the container lid slides to be changeable between an opened situation wherein the reagent is accessible from an outside and a closed situation wherein the reagent is prevented from being accessed from the outside, wherein the chilled reagent container further comprises a reagent container packing including another hole through which the reagent vessels are accessible and arranged between the container lid and the reagent vessel containing part to be pressed against the container lid.
Abstract: A mechanism is provided for manipulating a molecule. The molecule is driven into a nanochannel filed with electrically conductive fluid. A first vertical electric field is created inside the nanochannel to slow down the molecule and/or immobilize the molecule. The molecule is stretched into non-folded linear chains by the first vertical electric field and a horizontal electric field. Monomers of the molecule are sequentially read.
Type:
Grant
Filed:
November 30, 2012
Date of Patent:
December 9, 2014
Assignee:
International Business Machines Corporation
Abstract: The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.
Abstract: The present invention provides for the detection of biochemical analytes by measuring variations in ionic conductance resulting from the hybridization of a biological analyte (e.g. oligonucleotides) with a capturing element immobilized on a membrane substrate having nano-sized pores.
Abstract: A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.
Type:
Grant
Filed:
July 28, 2010
Date of Patent:
December 9, 2014
Assignees:
The University of Tokyo, Mitsubishi Chemical Medience Corporation
Abstract: In an aspect of the disclosure, a stimulation device includes a probe attached to a first support. The probe includes at least one grating coupler for coupling light into the probe. The device further includes at least one optical source for providing an optical stimulation signal mounted on a second support, and at least one means for detachably attaching the first support to the second support. The position of the at least one optical source is aligned with the position of the at least one grating coupler to allow light emitted from the at least one optical source to be received by the at least one grating coupler.
Type:
Application
Filed:
May 29, 2014
Publication date:
December 4, 2014
Applicants:
IMEC, KATHOLIEKE UNIVERSITEIT LEUVEN, KU LEUVEN R&D
Inventors:
Luis Diego Leon Hoffman, Dries Braeken, Silke Musa
Abstract: The application describes an MRD-based reactor. The reactor is characterized by a continuous wall portion, and is in connection with a MRD, adapted for performing localized NMR spectroscopy of the medium inside the reactor. MRD-based reactors, in which the MRD is at least partially inside the reactor or reaction media, and those in which the MRD accommodates the reactor, are also introduced. Lastly, the invention teaches an in situ method for controlling and analyzing of a reaction. The method makes use of an MRD-based reactor; and comprises applying a magnetic field within the reactor, especially for performing a plurality of localized spectroscopic measurements and either real time or offline analyzing and/or controlling of reactions in the flowing media.
Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first shell (16) disposed on a front face (5) of said base (2) and a second shell (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20).
Abstract: The invention provides reagents and methods for lateral flow assays and quantitative capture or determination of components, including cells, in a sample. In one aspect, reagents and methods for diagnostic assay are provided. In one embodiment an assay for determining T cell numbers, particularly a CD2+ CD4+ T cell assay is provided. A manufacturing method for producing rapid diagnostic assays in a decentralized manner is also described. The method generates net economic advantages over conventional diagnostic manufacturing practices.
Type:
Grant
Filed:
September 17, 2010
Date of Patent:
December 2, 2014
Inventors:
Michael J. Lane, Brian D. Faldasz, Jerrie Gavalchin
Abstract: Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable matrices are described for use as supports of biological samples, which matrices can be dried and subsequently rehydrated for sample recovery. Also disclosed are computer-implemented systems and methods for managing sample data.
Abstract: The present invention relates to the diagnosis and treatment of cancer, and in particular breast cancer. Specifically, in some embodiments the invention relates to methods of diagnosing cancer, and in particular breast cancer, using an antibody specific for a gene product that localizes selectively to the endoplasmic reticulum of the cancer cell(s). In some embodiments, the invention relates to methods of treating cancer, and in particular breast cancer, by administering a composition comprising an RNA interference sequence (e.g., shRNA, RNAi and/or siRNA molecule) characterized by an ability to inhibit an mRNA molecule, which mRNA molecule is encoded by the C43 gene (SEQ ID NO: 1). The invention additionally relates to methods for detecting cancer cells by detecting reduced methylation of the C43 promoter, and methods for reducing cancer metastasis by using demethylation inhibitors that result in increased methylation of the C43 promoter.
Type:
Grant
Filed:
July 20, 2009
Date of Patent:
December 2, 2014
Assignee:
The Research Foundation for the State University of New York
Abstract: The current invention describes in vivo and vitro (cultured) sampling technologies that allow direct temporal and spatial sampling from living ecosystems such as those associated with marine ecology. The optional use of parallel sampling methods, observatory design, provides for the ability to measure the response of individual organisms to a variety of both biotic and abiotic stresses. Sampling in small volumes and close proximity to living organisms has allowed direct measurement of various invertebrate and other aquatic species in marine ecosystems. These sampling techniques are intended to apply to any liquid based ecosystem in an attempt to minimize sampling as a dependent variable in measuring the chemical and biological behavior of the ecosystem. If is intended that this sampling technology be used to directly measure the chemical behavior of a wide variety of organisms; including, plants, animals, and micro-organisms (e.g. algae, plankton).
Abstract: An assay device includes: a liquid sample zone; a reagent zone downstream and in fluid communication with the sample zone containing a reagent material; a detection zone in fluid communication with the reagent zone having capture elements bound thereto; and a wicking zone in fluid communication with the capture zone having a capacity to receive liquid sample flowing from the detection zone. The sample receiving zone, the reagent zone, the detection zone and the wicking zone define a fluid flow path and at least a part of the fluid flow path has a substrate and projections which extend substantially vertically from the substrate, wherein the projections have a height, cross-section and a distance between one another that defines a space between the projections capable of generating capillary flow parallel to the substrate surface. In addition, the fluid flow path having projections includes a corner section which changes the direction of the flow path.
Type:
Grant
Filed:
January 18, 2013
Date of Patent:
November 25, 2014
Assignee:
Ortho-Clinical Diagnostics, Inc.
Inventors:
James D. Kanaley, Zhong Ding, Philip C. Hosimer, Edward R. Scalice, Susan Danielson, David A. Tomasso, Timothy C. Warren
Abstract: Enzyme based analyte sensors having radiation stabilizing agents are disclosed and described. More particularly, devices comprising a radiation stabilizing agent and methods for stabilizing sensors to high energy radiation sterilization are disclosed and described.
Abstract: A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed.
Type:
Grant
Filed:
April 9, 2009
Date of Patent:
November 25, 2014
Assignee:
Valtion Teknillinen Tutkimuskeskus
Inventors:
Hans Söderlund, Ari Hokkanen, Kari Kataja, Ingmar Stuns, Kai Kolari, Heli Siren, Stella Rovio, Reetta Satokari, Jan Rautio
Abstract: Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.
Type:
Grant
Filed:
March 7, 2013
Date of Patent:
November 25, 2014
Assignees:
IDEXX Laboratories, Inc., Divergence, Inc.
Inventors:
David Allen Elsemore, Jinming Geng, Laurie A. Flynn, Michael Crawford
Abstract: Device and method for detecting the presence of known or unknown toxic agents in a fluid sample. Targets in the sample are bound to releasable receptors immobilized in a reaction region of a micro- or nano-fluidic device. The receptors are selected based on their affinity for classes of known toxic agents. The receptors are freed and the bound and unbound receptors separated based on differential electrokinetic mobilities while they travel to a detection device.
Type:
Grant
Filed:
May 10, 2011
Date of Patent:
November 25, 2014
Assignee:
STC.UNM
Inventors:
Gabriel Lopez, Linnea Ista, Steven RJ Brueck, Aurelio Evangelista Lara, Mangesh Bore
Abstract: The present invention provides, among others, apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material.
Abstract: An apparatus for cultivating cells utilizing wave motion comprising a container, a retaining member configured to retain the container, a drive assembly for swiveling the container with respect to the substantially horizontal pivot axis and to swivel, such that during swiveling the pivot axis follows a cyclical closed-loop path.
Type:
Grant
Filed:
December 27, 2007
Date of Patent:
November 18, 2014
Assignee:
Strix Beheer B.V.
Inventors:
Pieter van der Heiden, Marc Buevink, Nicolaas Marius Gerard Oosterhuis
Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.
Type:
Grant
Filed:
January 20, 2011
Date of Patent:
November 18, 2014
Assignees:
California Institute of Technology, University of Southern California
Inventors:
Emil P. Kartalov, Darryl Shibata, Clive Taylor, Lawrence A. Wade
Abstract: A device and method for performing a point of care diagnostic test for detecting and quantifying at least one analyte in a biological sample (e.g., a body fluid). A device may include an immunoassay apparatus and a holder with an adjustable variable angle stage for positioning the immunoassay apparatus relative to a light source and a detector device so as to optimize the angle of incidence and angle of radiation to optimize an elastic light scattering signal from the immunoassay apparatus. The elastic light scattering signal may be used to quantify the amount of the analyte(s) of interest present in the sample. The device is based upon elastic light scattering, so the variation in the angle of incidence and angle of reflection are optimized to maximize signal generation due to elastic light scattering.
Type:
Grant
Filed:
September 12, 2012
Date of Patent:
November 18, 2014
Inventors:
Joel R. L. Ehrenkranz, Pamela J. Turbeville, Jeong-Yeol Yoon, David J. You
Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.
Abstract: A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and a biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits an amplified and detectable signal when it reacts with the specific infectious agent. Signal detection is enhanced through the release of LPS from the cells of the infectious agent.
Type:
Application
Filed:
March 13, 2014
Publication date:
November 13, 2014
Applicant:
APPLIED BIOMOLECULAR TECHNOLOGIES
Inventors:
Thomas J. ZUPANCIC, J.D. KITTLE, Lingchun ZENG, Srikanth VEDAMOORTHY, Min MO
Abstract: Disclosed herein are an apparatus and method for measuring biomedical data and a measurement strip. The apparatus includes a plurality of detection units arranged within a strip reception area on a plane and spaced apart from each other, a measurement type determination unit for determining whether reactive portions are present in areas of the measurement strip corresponding to the plurality of detection units based on detection results obtained by the detection units and determining a type of measurement based on results of the determination, a biomedical data measurement unit for activating part or all of the detection units according to the type of measurement determined by the measurement type determination unit, and measuring the biomedical data using the activated detection units, and an output unit for outputting the measured biomedical data to an outside.
Abstract: The potentiometric device and method selective for pioglitazone relates to the detection of pioglitazone in urine, in other liquid biological samples, and in pharmaceutical preparations for quality control testing and the like, and particularly to the use of a potentiometric sensor for potentiometric detection and measurement of the concentration of pioglitazone. The potentiometric sensor includes a plasticized polyvinyl chloride (PVC) matrix membrane having an ionophore impregnated or embedded therein. The ionophore is an iodobismuth anion in which the iodobismuth anion forms a complex with pioglitazone. The polymer membrane is plasticized with either ortho-nitrophenyl octyl ether (NPOE) or dioctyl phthalate (DOP).
Abstract: Glucose and ATP biosensors have important applications in diagnostics and research. Combining single-walled carbon nanotubes (SWCNTs) with Pt nanoparticles can significantly enhance the performance of electrochemical biosensors. This disclosure illustrates the use of single-stranded DNA (ssDNA) to modify SWCNTs to increase SWCNT solubility in water. Multiple embodiments with this configuration allows for exploration of new schemes of combining ssDNASWCNT and Pt black in aqueous media systems. These embodiments resulted in a nanocomposite with enhanced biosensor performance. The ssDNA-SWCNT/Pt black nanocomposite constructed by a layered scheme proved most effective in terms of biosensor activity. The key feature of this structure and method of use is the exploitation of ssDNASWCNTs as molecular templates for Pt black electrodeposition. Glucose and ATP microbiosensors fabricated utilizing this structure and method of use exhibited high sensitivity, wide linear range and low limit of detection.
Type:
Grant
Filed:
September 14, 2012
Date of Patent:
November 11, 2014
Assignee:
Purdue Research Foundation
Inventors:
D. Marshall Porterfield, Tae-Gon Cha, Jong Hyun Choi, Jonathan C. Claussen, Alfred R. Diggs, Jin Shi
Abstract: The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.
Type:
Grant
Filed:
April 8, 2009
Date of Patent:
November 11, 2014
Assignee:
Nexcelom Bioscience LLC
Inventors:
Bo Lin, Peter Y. Li, Jean Qiu, Timothy Smith, Todd Sobolewski, Alnoor Pirani
Abstract: The present invention relates to a novel biosensor. A resonance energy transfer (RET) biosensor comprising a beta(?)-arrestin tagged with a first and a second chromophore, wherein said first chromophore is a fluorophore and said second chromophore is a fluorophore or a bioluminophore is described.
Type:
Grant
Filed:
April 22, 2011
Date of Patent:
November 11, 2014
Assignee:
Universite de Montreal
Inventors:
Michel Bouvier, Pascale G. Charest, Christian LeGouill, Alexandre Beautrait, Stephane Alain Laporte, Brandon Zimmerman
Abstract: Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor adjacent to the pore configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle, thereby detect
Abstract: A new device and methods that allow for improved sequestration and preservation of harvested analytes and bio-molecules from biofluid samples is defined. The new device and methods relate to an improved dried biofluid collection substrate that is absorbent and contains a plurality of affinity ligands located within defined sample collection regions for enhanced analyte collection and storage. The device and methods allow for simple, safe and reliable ambient temperature collection and preservation of molecules captured from biological and environmental fluids in quantities suitable for analysis and diagnostic testing.
Abstract: A system for measuring a solution pH includes a potentiostat with a working electrode made of an electro-conductive solid polymer transducer, an input to receive the potential to be applied between the working electrode and a reference electrode, and an output to transmit a signal representative of the current flowing between a counter electrode and the working electrode, the three electrodes being immerged into the solution. The system further includes a digital processor connected to a digital to analog converter for generating the potential to be applied between the working and the reference electrodes; and to an analog to digital converter for receiving a digital value representative of the current. The digital processor is adapted to modify the potential to maintain the current inside a predetermined range such that the potential is representative of the solution pH when the current is inside the predetermined range.
Type:
Grant
Filed:
June 2, 2010
Date of Patent:
November 4, 2014
Assignee:
Universite Catholique de Louvain
Inventors:
Sami Yunus, Patrick Bertrand, Anne Attout
Abstract: The invention relates to methods and kits for performing in situ hybridization on a biological sample on a solid surface using nucleic acid probes that are embedded in or sorbed to a dry, fibrous matrix.
Type:
Grant
Filed:
October 16, 2013
Date of Patent:
November 4, 2014
Assignee:
Cellay, Inc.
Inventors:
Joan Aurich-Costa, Elizabeth Ewen, Michael Gildea
Abstract: There is provided an assay device comprising a lid and a base, said base comprising, a sample addition zone, a reaction zone and an absorbing zone, said components being in fluid connection and being part of a fluid passage leading from the sample addition zone to the absorbing zone, wherein: (a) a sample addition well is integrated in the lid, (b) the absorbing zone consists of an area on an non-porous substrate, having substantially perpendicular projections, said projections defining a volume, which together with the volume of the fluid passage defines the sample volume subjected to the assay, and (c) at least one filter is between the sample addition well and the sample addition zone. There is further provided methods for handling samples.
Abstract: Embodiments of the present disclosure set forth a biosensor for detecting a target. One example sensor includes a first electrode. The first electrode includes a first electron conducting molecule and a first probe. The first probe includes a second electron conducting molecule. The first probe is configured to bind to the target of interest in solution. The first and second electron conducting molecules are different.
Type:
Grant
Filed:
June 21, 2011
Date of Patent:
November 4, 2014
Assignee:
National Taiwan University
Inventors:
Shiming Lin, Bor-Ching Sheu, Luan-Yin Chang, Chih-Chen Lin
Abstract: Methods for determining the hematocrit of a blood sample, and devices and systems used in conjunction with the same. The hematocrit value can be determined on its own, and further, it can be further used to determine a concentration of an analyte in a sample. In one exemplary embodiment of a method for determining the hematocrit value in a blood sample, a volume of blood is provided in a sample analyzing device having a working and a counter electrode. An electric potential is applied between the electrodes and an initial fill velocity of the sample into the device is calculated. The hematocrit of the blood, as well as a concentration of an analyte in view of the initial fill velocity can then be determined. Systems and devices that take advantage of the use of an initial fill velocity to determine hematocrit levels and make analyte concentration determinations are also provided.
Type:
Grant
Filed:
December 30, 2009
Date of Patent:
November 4, 2014
Assignee:
LifeScan, Inc.
Inventors:
Ronald C. Chatelier, Dennis Rylatt, Linda Raineri, Alastair M. Hodges
Abstract: An electrochemical-based analytical test strip for the determination of an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) and/or a characteristic of the bodily fluid sample (for example, hematocrit) includes a first sample-receiving chamber with first and second sample-application openings, and first and second electrodes. The first and second electrodes are disposed in the first sample-receiving chamber between the first and second sample-application openings. The electrochemical-based analytical test strip also includes a second sample-receiving chamber and a plurality of electrodes disposed in the second sample-receiving chamber. In addition, the second sample-receiving chamber intersects the first sample-receiving chamber between the first and second electrodes, thereby defining a chamber intersection.
Type:
Grant
Filed:
June 21, 2012
Date of Patent:
November 4, 2014
Assignee:
Lifescan Scotland Limited
Inventors:
Lynsey Whyte, Scott Sloss, Neil Whitehead, David McColl, Antony Smith
Abstract: Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample.
Abstract: Provided is a device and a method for examining myocardial toxicity, which can be realized in vitro in an equivalent manner as those conventionally carried out in vivo. A cell population as a pulsating pacemaker is arranged on a transparent substrate. Myocardial pulsating cells are arranged while being spaced apart appropriately. Fibroblast cells are arranged with/connected to the myocardial pulsating cells to form a cell network. Each of the myocardial pulsating cells and fibroblast cells forming the network is arranged on a transparent electrode provided on the transparent substrate. The cells forming the network are exposed to a flow of a solution containing a drug and QT delay due to the drug is evaluated.
Type:
Grant
Filed:
December 4, 2009
Date of Patent:
November 4, 2014
Assignee:
National University Corporation Tokyo Medical and Dental University
Abstract: The invention provides a method of measuring the affinity of first and second biomolecules in which a first biomolecule is tethered by a first tether portion having a first tether portion length and a second biomolecule is tethered by a second tether portion having a second tether portion length, the method comprising determining binding of adjacent first and second biomolecules to each other, varying at least one of the first and second tether lengths and determining binding of the first and second biomolecules. The invention also provides apparatus suitable for use in the method of the invention.
Type:
Application
Filed:
March 21, 2014
Publication date:
October 30, 2014
Inventors:
Trevor Clive DALE, Adrian John HARWOOD, Paola BORRI
Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for diagnosing pancreatic cancer in a subject, a method for identifying whether a subject is in need for a therapy of pancreatic cancer or a method for determining whether a pancreatic cancer therapy is successful. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices.
Type:
Application
Filed:
November 29, 2012
Publication date:
October 30, 2014
Applicant:
METANOMICS HEALTH GMBH
Inventors:
Regina Reszka, Beate Kamlage, Holger Kalthoff, Bodo Schniewind, Julia Mayerle, Markus M. Lerch, Christian Pilarsky, Robert Grützmann
Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.
Type:
Grant
Filed:
November 1, 2011
Date of Patent:
October 28, 2014
Assignee:
Caliper Life Sciences, Inc.
Inventors:
Dan Camporese, Advit Bhatt, Josh Molho, Hui Xu, Ken Summers
Abstract: The present invention relates to systems, methods, and devices for determining the concentration of an analyte in a sample. The use of linear, cyclic, or acyclic voltammetric scans and/or semi-integral, derivative, or semi-derivative data treatment may provide for increased accuracy when determining the concentration of an analyte in a sample. Hematocrit compensation in combination with the data treatments may reduce the hematocrit effect with regard to a glucose analysis in whole blood. In another aspect, fast scan rates may reduce the hematocrit effect.