Abstract: Dextransucrase synthesis of dextran from sucrose is inhibited by a novel homocitric acid oligoriboside derivative and its nontoxic salts. The new derivative is obtained by fermentation of certain Streptomyces sp. such as Streptomyces sp. MF 980-CF1 (FERM-P5430; ATCC 31820) and is useful in the prevention of dental caries.

Abstract: Anthracycline glycosides W, X, Y and Z which are prepared by the fermentation of mutant F.I. 416 of Streptomyces peucetius var. caesius. The new compounds are useful against both gram positive and gram negative bacteria and as antitumor agents.

Abstract: The present invention discloses selectable pairs of recombinant DNA cloning vectors for use in Streptomyces and related organisms.

Abstract: Antibiotic A-39183 complex, comprising microbiologically active, related factors A, B, C, D, and E, is produced by submerged, aerobic fermentation of a new microorganism Streptomyces sp., NRRL 12049. The A-39183 antibiotics are closely related antibiotics. The individual A-39183 factors are separated by chromatography. The A-39183 factors are antibacterial agents which have activity against Staphylococcus and Streptococcus species that are penicillin resistant. The A-39183 factors are also active against both gram-positive and gram-negative anaerobic bacteria, and are ionophores.

Abstract: Compounds of formula (I): ##STR1## (wherein R represents a group of formula ##STR2## and the corresponding ring-closed lactones, salts (especially alkali metal salts) and esters (especially C.sub.1 -C.sub.5 alkyl esters) thereof may be prepared by subjecting ML-236B, or ML-236B carboxylic acid or a salt or ester thereof to enzymatic hydroxylation, which may be effected by means of microorganisms of the genera Mucor, Rhizopus, Zygorynchus, Circinella, Actinomucor, Gongornella, Phycomyces, Martierella, Pycnoporus, Rhizoctonia, Absidia, Cunninghamella, Syncephalastrum and Streptomyces, or cell-free, enzyme-containing extracts from said microorganisms. The compounds are capable of inhibiting biosynthesis of cholesterol and are thus useful in the treatment of hypercholesteraemia.

Abstract: 7.alpha.-Methoxy-3-p-sulfooxy or p-hydroxycinnamoyloxymethyl cephalosporin derivatives which are useful as antibiotics and as intermediates for the production of other 7.alpha.-methoxycephalosporin derivatives.

Abstract: There is presented the compounds 4-deoxy-aclacinomycin A and B and pharmaceutical preparations containing the same. The preparations are effective against bacteria and tumours. Also presented is a process for the preparation of optically active anthracycline glycosides A and B with the 7S-configuration starting from racemic anthracyclinones.

Abstract: Angiotensin I converting enzyme inhibitor A-58365 factor B is provided in greater abundance than coproduced Factor A by culturing Streptomyces chromofuscus NRRL 15098 under submerged aerobic fermentation conditions in an aqueous nutrient culture medium supplement with the amino acids proline and lysine.

Abstract: Angiotensin I converting enzyme inhibitor A-58365 factor A is produced in enhanced yields by culturing Streptomyces chromofuscus NRRL 15098 under submerged aerobic fermentation conditions in a proline supplemented aqueous nutrient culture medium containing assimilable sources of carbon, nitrogen and inorganic salts.

Abstract: A process for preparing a B30-threonine-insulin which comprising reacting a des-B30-insulin with an excess amount of threonine derivative in the presence of an enzyme specifically acting on the basic-amino-acid carbonyl in peptide bondings.

Abstract: Acidophilic and acidoduric streptomycetes strains have been found to produce carbohydrases. These Streptomyces effectively elaborate glucose isomerase under acid conditions typically unfavorable for growth of conventional glucose isomerase producing Streptomyces. Sterilization of the culture and production media may be avoided by selectively propagating newly discovered Streptomyces acidodurans under acidic conditions which will effectively eliminate contaminating microorganisms. The Streptomyces acidodurans herein also have the ability to undergo cultivation and elaborate glucose isomerase over a relatively broad pH range. Constitutive streptomycetes strains have also been isolated. Glucose isomerases derived from these Streptomyces strains are particularly effective for isomerizing glucose syrups to fructose-containing syrups.

Abstract: Novel antibiotic U-64,767 producible in a fermentation under controlled conditions using a biologically pure culture of the microorganism Streptomyces macronensis Dietz sp.n., NRRL 12566. This antibiotic is active against various Gram-positive bacteria, for example, Staphylococcus aureus and Streptococcus pyogenes. It is also active against the Gram-negative bacterium Streptococcus pneumoniae. Thus, antibiotic U-64,767 can be used in various environments to eradicate or control such bacteria.

Abstract: The novel antibiotic, oxanosine, having the structure ##STR1## inhibits the growth of Gram-negative bacteria and has antiviral and carcinostatic activity. It is produced by cultivation of an oxanosine-producing microorganism of the genus Streptomyces, preferably Streptomyces capreolus MG265-CF3, ATCC No. 31963.

Abstract: Novel chemical compound plasmid pUC1061 obtained by deletion of .about.2.0 kilobases of DNA from the Streptomyces espinosus plasmid pUC6. This plasmid is useful as a cloning vehicle in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the glucose isomerase gene, can be inserted into the plasmid and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired glucose isomerase.

Abstract: This disclosure describes a novel process for the production of the known antibiotic nosiheptide using a new strain of Streptomyces glaucogriseus and mutants thereof.

Abstract: There is presented a process for producing anthracycline glycosides A by converting the sugar moiety of anthracycline glycosides B, represented by the following formula: ##STR1## into the sugar moiety of anthracycline glycosides A, represented by the following formula: ##STR2## with the aid of a microorganism belonging to the species Streptomyces galilaeus or Streptomyces melanogenes.

Abstract: Four new antibiotics which are denominated istamycin A, istamycin B, istamycin A.sub.o and istamycin B.sub.o, and which are useful as antibacterial agents, are produced by fermentation of a new microorganism, Streptomyces tenjimariensis.

Abstract: A method of increasing the yield of a desired product, such as an antibiotic, by an organism which normally must first become inducibly resistant to that product before it can produce the product in maximum yields comprises producing constitutively resistant cells of the organism by supplementing a culture of the organism with an agent in which only cells able to specifically modify the 23S ribosomal RNA constitutively, rather than inducibly, survive thereby producing an organism in which the resistance to the product is expressed without the need for activation by the induction process; followed by purification and utilization of that organism for increased product production.

Abstract: The present invention relates to a process for producing aclacinomycins A and B. More particularly, the present invention is concerned with a process for producing selectively aclacinomycins A and B in high yield without formation of cinerubins A and B, by means of a fermentation process, utilizing Streptomyces galilaeus OBB-731.

Abstract: A new process for preparing thienamycin, a known antibiotic useful as a medicament and veterinary drug, characterized by cultivating Streptomyces penemifaciens sp. nov. in a suitable medium and recovering thienamycin from the fermentation broth.

Abstract: Novel alkaline protease M.sub.3 which is distinguished from known alkaline proteases in the phisiochemical properties, particularly in terms of the strong alkaline optimum pH: 9-12.5; being not inhibited by FeSO.sub.4, diisopropyl fluorophosphate, ethylenediamine tetraacetate and iodoacetic acid; having glucosyltransferase inhibitory activity; and having the lowest molecular weight, and which can be produced by cultivating a microorganism of the genus Streptomyces, particularly Streptomyces globisporus B-1829 strain (ATCC 21553). The alkaline protease M.sub.3 is useful for prevention of dental caries.

Abstract: A new acidic polycyclic ether antibiotic has the formula: ##STR1## and can be prepared by the submerged aerobic propagation in aqueous nutrient media of Streptomyces halstedii ATCC 31812. The antibiotic and its cationic salts are active against a variety of microorganisms and are effective in controlling coccidiosis, enteritis, swine dysentery and theileriosis as well as being effective in promotion of growth in poultry and ruminants.

Abstract: A polycyclic antitumor antibiotic designated herein as anandimycin is produced by fermentation of Streptomyces anandii subsp. araffinosus strain C-22,437 (ATCC 31431). Anandimycin possesses antibacterial activity and inhibits the growth of tumors in experimental animals.

Abstract: Antibiotic compounds of the formula: ##STR1## wherein R' is ##STR2## or --CH.sub.2 OH, and the non-toxic pharmaceutically acceptable acid addition salts thereof, are produced by culturing Streptomyces flocculus NRRL 11459. Techniques for isolating the compounds are also described. Cirramycin A.sub.1 and cirramycin B are coproduced.

Abstract: Zinc complexes of mononitrogen-containing divalent pyrrole ether antibiotics are provided. Such complexes act as coccidiostats and growth-promoting agents when administered to poultry and act as active agents for improving cardiovascular function in animals.Zinc complexes of the type disclosed can be prepared by adding soluble zinc salt to a fermentation beer containing the mononitrogen-containing pyrrole ether antibiotic to thereby form an insoluble, recoverable biomass containing the desired zinc antibiotic complex.

Abstract: A group of novel depsipeptide antibiotics, neoviridogriseins I, II and III are produced by fermentation of Streptomyces sp. P8648 (FERM-P 3562; ATCC 31289). The antibiotics are highly active against Gram-positive bacteria and mycoplasmas.

Abstract: The present invention teaches a new antibiotic nanaomycin E represented by the formula: ##STR1## Nanaomycin E is active against Gram-positive bacteria, Trichophyton and Mycoplasma and may be used as antibacterial and therapeutic agents for humans and animals. Nanaomycin E is also a useful starting material for the preparation of nanaomycin A which latter nanaomycin has the highest activity among the various nanaomycin-type compounds. Nanaomycin E is produced by fermentation of a microorganism belonging to the genus Streptomyces and capable of producing nanaomycin E, especially Streptomyces rosa variant notoensis (FERM-P 2209; ATCC 31135) and recovering the same from the fermented liquor.

Abstract: There is provided a process for producing glucose-isomerase in high yield by culturing under aerobic conditions, the strain Streptomyces griseoflavus (NCIB 11542).

Abstract: A method of obtaining glucose isomerase which comprises cultivating the enzyme-producing strain Streptomyces sp. N.765, registration No. 143 (Bulgarian State Institute for Drug Control, Sofia, Bulgaria,) for 36 to 72 hours at a temperature of 24.degree. to 36.degree. C. at an initial pH of 6.5 to 9.0 in a culture medium containing 1.0 to 2.0% xylose 1.5 to 4.0% of dry weight of maize extract and 0.23 to 1.0% weight sodium acetate.

Abstract: A novel glycerol kinase is produced from the microorganism Streptomyces canus FERM-P No. 4977 and is useful as a diagnostic reagent for assay of triglycerides and glycerol in body fluids such as serum.

Abstract: The new microorganism Streptomyces granuloruber, NRRL 12389 and the fermentation process for preparing narasin by submerged aerobic fermentation of this organism.

Abstract: Novel chemical compounds, cointegrate plasmids pUC1012 and pUC1013, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6, and plasmids pUC1015 and pUC1022 which are obtained by restructuring plasmid pUC1012, and plasmids pUC1016 and pUC1023 which are obtained by restructuring plasmid pUC1013. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

Abstract: Novel chemical compounds, recombinant plasmids pUC1026 and pUC1027, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6. These plasmids are produced by a novel process which can be used to stabilize unstable potential plasmid vectors. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin. The stabilization process disclosed herein can be used to make other stable plasmids.

Abstract: The present invention provides a process for obtaining cholesterol oxidase, wherein Streptomyces griseofuscus DSM 40191, Streptomyces hygroscopicus 40771, Streptomyces acidomyceticus DSM 40798 and/or Arthrobacter paraffinens DSM 312 are cultured and the enzyme obtained from the culture supernatant and/or the cells.

Abstract: Novel substance M-9337 obtained by culturing Streptomyces antihaemolyticus is effective as an antitoxic substance for neutralizing toxins discharged from streptococci, staphylococci, tetanus or the like.

Abstract: A process for producing an antibiotic Cephamycin C which comprises cultivating a strain of Streptomyces sp. OFR 1022 in a culture medium to accumulate therein Cephamycin C and recovering said Cephamycin C.

Abstract: Novel antibiotic U-62,162 producible in a fermentation under controlled conditions using a man-made biologically pure culture of the microorganism Streptomyces verdensis, Dietz and Li sp.n., NRRL 12256. This antibiotic is strongly active against various Gram-positive bacteria, for example, Staphylococcus aureus. Thus, antibiotic U-62,162 can be used in various environments to eradicate or control such bacteria.

Abstract: An antibiotic substance KA-7038 selected from the group consisting of the compounds having formulae I to VII described in claim 1, mixtures thereof and acid addition salts thereof; an antibiotic composition composed of (i) antibiotically effective amount of aforesaid antibiotic substance KA-7038, and (ii) a pharmaceutically acceptable diluent or carrier. The antibiotic substance KA-7038 can be produced by cultivating an antibiotic substance KA-7038-producing strain belonging to genus Streptomyces, for example Streptomyces sp. KC-7038, and isolating the antibiotic substance KA-7038 from the culture broth.

Abstract: A process for preparing a B30-threonine-insulin which comprising reacting a des-B30-insulin with an excess amount of threonine derivative in the presence of an enzyme specifically acting on the basic-amino-acid carbonyl in peptide bondings.

Abstract: A method for producing glucose isomerase comprising the steps of cultivating the enzyme producing strain Streptomyces sp. 1339 registration N. 144 Bulgarian State Institute for Drug Control, for 36 to 72 hours in a culture medium with xylose as an indicator, the temperature being kept at 24.degree. to 36.degree. C., the initial pH of cultivation being from 6.5 to 9.0 and effective isomerization at a temperature of 50.degree. to 80.degree. C., and a pH of 6.0 to 9.0 in the presence of 1.times.10.sup.-4 M CoCl.sub.2.6H.sub.2 O 1.times.10.sup.-2 M MgSO.sub.4.7H.sub.2 O in 0.1 to 3 M of the substrate.

Abstract: The microorganism Streptomyces capillispira, NRRL 12279, produces an enzyme which deesterifies cephalosporin methyl esters.

Abstract: Antibiotic A-32256 which is produced by submerged, aerobic fermentation of a Streptomyces sp. NRRL 12067. The antibiotic has shown antibacterial activity against Staphylococcus and Streptococcus and various anaerobic species. In addition, the antibiotic has shown antitrichomonas activity in vitro, as well as activity for improving ruminant feed efficency and as a mosquito larvacide.

Abstract: Prodn. of FR-900129 comprises culturing a Streptomyces n. 4012 category which is analogous to Streptomyces misakiensis and Steptomyces aburaviensis, i.e. Streptomyces avellaneus No. 4012, at 25.degree. C. to 30.degree. C. during 50 to 100 hours in a culture medium which consists of a carbon source (e.g. glucose, fructose, glycerin, starch, galactose, maltose, dextrin), an organic or inorganic nitrogen source (e.g. yeast extract, peptone, cottonseed cake, soyabean powder, cornsteep liquor, dry yeast, ammonium nitrate, ammonium sulphate, ammonium phosphate, urea and amino acid) and an inorganic salt (e.g. calcium carbonate, sodium phosphate, potassium phosphate, sodium chloride, potassium chloride, magnesium salt and copper salt). The produced FR-900129 is isolated by a collecting step, a purifying step, a vacuum condensation step, a freeze-drying step, a pH adjusting step, a cationic, anionic or nonionic resin treating step, an active carbon adsorption step and a crystallizing and recrystallizing step.

Abstract: The new microorganism Streptomyces lydicus DeBoer et al., NRRL 12034 and the fermentation process for preparing narasin by submerged aerobic fermentation of this organism.

Abstract: Novel antibiotic U-60, 394 producible in a fermentation under controlled conditions using a biologically pure culture of the microorganism Streptomyces woolenses, Dietz and Li sp.n., NRRL 12113. This antibiotic is strongly active against various Gram-positive bacteria, for example, Staphylococcus aureus and Streptococcus hemolyticus. It is also strongly active against the Gram-negative bacterium Streptococcus pneumoniae. Thus, antibiotic U-60, 394 can be used in various environments to eradicate or control such bacteria.

Abstract: There is disclosed a new antibiotic agent, A73A, produced by Streptomyces viridifaciens MA-4864 (ATCC 31495) under suitable conditions. Said antibiotic shows antibacterial and growth-permittant activity.

Abstract: A novel antibiotic having the ascribed formula ##STR1## is provided. They physical characteristics are as follows: (a) Elementary analysis: Calculated (for C.sub.20 H.sub.16 O.sub.7): C,65.22; H,4.35%. Found: C,64.60; H,4.34%.(b) Molecular weight (mass-spectrometric): 368.(c) Melting point: 225.degree.-226.degree. C.(d) Specific rotation: [.alpha.].sub.D .sup.20 =+123.5.degree. (c=0.2 in chloroform).(e) Ultraviolet absorption spectrum:.lambda..sub.max .sup.methanol =235 nm (.epsilon.=28600); 262 nm (.epsilon.=21900); 434-435 nm (.epsilon.=11300);.lambda..sub.max .sup.0.1-N NaOH/methanol =246 nm (.epsilon.=22400); 256 nm (.epsilon.=23200); 523-524 nm (.epsilon.=9600).(f) Infrared absorption spectrum: 3500, 1710, 1672, 1628, 1600, 1575, 1472, 1452, 1420, 1390, 1295, 1255, 1218, 1200, 1182, 1105, 1025, 1005, 962, 938, 848, 765, 752, 730 cm.sup.-1.

Abstract: Disclosed are optically active cephalosporin analogs which are produced by optically selective deacylation of an optically inactive acylated analog. The compounds are useful as intermediates in the preparation of optically active acylated antimicrobial agents.

Abstract: A known antibiotic substance designated herein as rachelmycin is produced by fermentation of Streptomyces sp. strain C-329, variant 70 (ATCC 31128) or Streptomyces anandii subsp. arraffinosus strain C-22, 437 (ATCC 31431). Rachelmycin exhibits both antibiotic and antitumor properties.

Abstract: Antibiotic A-21978 complexes, in particular the A-21978C complex, comprising microbiologically active, related factors C.sub.0, C.sub.1, C.sub.2, C.sub.3, C.sub.4, and C.sub.5. A-21978 complex and A-21978C complex are produced by submerged aerobic fermentation of Streptomyces roseosporus NRRL 11379. The individual A-29178C factors are separated and isolated by chromatography. The A-21978 and A-21978C complexes; the A-21978C factors; and pharmaceutically acceptable salts thereof are antibacterial agents and improve growth promotion in poultry.