Enzymatic preparation of monic acids
A process for the production of monic acids which comprises contacting a corresponding pseudomonic acid or an ester thereof with a hydrolase enzyme from a suitable microorganism, in particular Streptomyces sp.
Latest SmithKline Beecham p.l.c. Patents:
- AUTOMATED IMMUNOSSAY APPARATUS WITH FLEXIBLE PICK-UP ARM
- Aminopiperidine quinolines and their azaisosteric analogues with antibacterial activity
- N-aroyl cyclic amine derivatives as orexin receptor antagonists
- Ethylene diamine derivatives and their use as orexin-receptor antagonists
- Pyridine derivatives as Raf kinase inhibitors
Claims
1. An enzyme which hydrolyzes pseudomonic acid to monic acid, the enzyme obtained by treating mycelium from Streptomyces lividans NCIMB 11416, by centrifugation and cell disruption followed by fractionation and chromotagraphy.
2. An enzyme as claimed in claim 1 having a molecular weight of approx. 54,000 daltons.
3. An enzyme which hydrolyzes pseudomonic acid to monic acid, the enzyme obtained by treating mycelium from Kitosatosporia sp. NCIMB 40568, by centrifugation and cell disruption followed by fractionation and chromatography.
| 4284570 | August 18, 1981 | Rogers et al. |
| 0 003 069 | July 1979 | EPX |
| 0180285 | June 1989 | JPX |
| 1 587 059 | March 1981 | GBX |
- Freer et al. Proceedings 3.sup.rd Intl. Symp., Elsevier, 1988 pp. 295-298. Sime et al. Tet. Lett. Vol. 28(43) pp. 5169-5172 1987. Mantle et al. Appl. Microbiol. Biotechnol. vol. 33, pp. 709-711 1990.
Type: Grant
Filed: Feb 15, 1996
Date of Patent: Mar 10, 1998
Assignee: SmithKline Beecham p.l.c. (Brentford)
Inventors: Esme Faith Hayes (Worthing), John Thomas Sime (Dorking), Stefan Roland Woroniecki (Horsham), David Alan Yeandle (Lancing)
Primary Examiner: Leon B. Lankford, Jr.
Assistant Examiner: Christopher R. Tate
Attorneys: William T. King, Edward T. Lentz
Application Number: 8/578,587
International Classification: C12N 914; C12N 916;
