Preparing Alpha Or Beta Amino Acid Or Substituted Amino Acid Or Salts Thereof Patents (Class 435/106)
  • Publication number: 20100330623
    Abstract: The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.
    Type: Application
    Filed: February 19, 2009
    Publication date: December 30, 2010
    Applicant: SCARAB GENOMICS LLC
    Inventors: Frederick R. Blattner, Sun Chang Kim, Jun Hyoung Lee
  • Patent number: 7858349
    Abstract: The invention provides bradykinin antagonists and pharmaceutically acceptable salts thereof having anti-cancer activity. These anti-cancer compounds are particularly useful for inhibiting the growth of lung and prostate cancers.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: December 28, 2010
    Assignee: The Regents of the University of Colorado
    Inventors: John M. Stewart, Daniel C. F. Chan, Lajos Gera, Paul A. Bunn, Jr.
  • Patent number: 7858348
    Abstract: The present invention provides genes that encode the N-acetyl-(R,S)-?-amino acid acylases. The N-acetyl-(R,S)-?-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-?-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-?-amino acid acylase produced by Variovorax sp.
    Type: Grant
    Filed: July 24, 2007
    Date of Patent: December 28, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Shunichi Suzuki, Yuki Imabayashi, Kunihiko Watanabe, Hisashi Kawasaki, Tsuyoshi Nakamatsu
  • Patent number: 7855060
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging the genus Escherichia or Pantoea, wherein said bacterium has attenuated expression of a gene encoding a toxin of a bacterial toxin-antitoxin pair.
    Type: Grant
    Filed: July 31, 2007
    Date of Patent: December 21, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremeteva, Aleksandra Yurievna Skorokhodova, Vitaly Grigorievich Paraskevov
  • Patent number: 7850852
    Abstract: According to the process for producing amino acid or salt thereof in the present invention, in the adsorption step, an amino acid-containing aqueous solution is fed into a pressure tight column so that a free amino acid is adsorbed on a carbonate-type anion exchange resin packed in the pressure tight column. Subsequently, in the elution step, eluent liquid containing a hydrogen carbonate ion and/or a carbonate ion is injected into the pressure tight column in a pressurized state to elute the amino acid adsorbed on the anion exchange resin and simultaneously to regenarate the anion exchange resin into the carbonate-type. In the case of purifying an acidic amino acid, an aqueous ammonium carbonate solution is employed as the eluent liquid. In the case of purifying a neutral amino acid, an aqueous carbonic acid solution, an aqueous hydrogen carbonate solution, an aqueous ammonium hydrogen carbonate solution or an aqueous ammonium carbonate solution is employed as the eluent liquid.
    Type: Grant
    Filed: August 24, 2007
    Date of Patent: December 14, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yasuhiro Kusunose, Kenji Takahashi
  • Publication number: 20100311129
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of a gene coding for sRNA.
    Type: Application
    Filed: August 18, 2010
    Publication date: December 9, 2010
    Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Tatyana Viktorovna Leonova, Vitaly Grigorievich Paraskevov, Takuji Ueda
  • Patent number: 7846698
    Abstract: The L-lysine-producing ability and the L-lysine-producing speed are improved in a coryneform bacterium harboring an aspartokinase in which feedback inhibition by L-lysine and L-threonine is substantially desensitized, by successively enhancing DNA coding for a dihydrodipicolinate reductase, DNA coding for a dihydrodipicolinate synthase, DNA coding for a diaminopimelate decarboxylase, and DNA coding for a diaminopimelate dehydrogenase.
    Type: Grant
    Filed: August 23, 2002
    Date of Patent: December 7, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Seiko Otsuna, Masakazu Sugimoto, Masako Izui, Atsushi Hayakawa, Eiichi Nakano, Masaki Kobayashi, Yasuhiko Yoshihara, Tsuyoshi Nakamatsu
  • Publication number: 20100304449
    Abstract: The invention relates to a method for producing different products comprising a target substance, particularly amino acids or vitamins, wherein the target substance is produced by fermentation.
    Type: Application
    Filed: December 11, 2007
    Publication date: December 2, 2010
    Inventors: Matthias Moll, Stefan Stockhammer, Hans Christian Alt, Joachim Pohlisch, Klaus Huthmacher
  • Publication number: 20100304448
    Abstract: A method for the enzymatic preparation of amino acids of the general formula (3) or (4) from alpha-dehydroamino acids of the general formula (1) or (2) wherein R1, R2 are independently of one another H, C1-C6 alkyl, C2-C6 alkenyl, an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or an alkylaryl radical, or a carboxyl radical (—COOR), R3 is H, formyl, acetyl, propionyl, benzyl, benzyloxycarbonyl, BOC, Alloc, R is H, C1-C6 alkyl, aryl, by reducing a compound of the formula (1) or (2) in the presence of a reductase.
    Type: Application
    Filed: December 8, 2008
    Publication date: December 2, 2010
    Inventors: Rainer Stürmer, Bernhard Hauer, Thomas Friedrich, Kurt Faber, Melanie Hall, Clemens Stückler
  • Patent number: 7833760
    Abstract: The present invention relates to a Variovorax sp. which produces an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a Burkholderia sp. which produces both an acylase having an asymmetric hydrolysis activity on an N-acetyl ?-amino acid to selectively produce an S-?-amino acid and an acylase having an asymmetric hydrolysis activity of an N-acetyl ?-amino acid to selectively produce an R-?-amino acid, and a process for the selective production of an S-, or R-?-amino acid using the above strains.
    Type: Grant
    Filed: October 26, 2007
    Date of Patent: November 16, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tsuyoshi Nakamatsu, Hisashi Kawasaki, Kunihiko Watanabe, Masakazu Nakazawa, Kunisuke Izawa
  • Patent number: 7833761
    Abstract: A microorganism is provided which has an ability to produce an L-amino acid such as L-lysine, L-tryptophan, L-phenylalanine, L-valine, L-leucine, L-isoleucine and L-serine, and has been modified to increase the activity of pyruvate synthase or pyruvate:NADP+ oxidoreductase. This microorganism is cultured in a medium containing ethanol or an aliphatic acid as the carbon source to produce and accumulate the L-amino acid in the medium or cells, and the L-amino acid is collected from the medium or the cells.
    Type: Grant
    Filed: September 2, 2008
    Date of Patent: November 16, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masaru Terashita, Yoshihiro Usuda, Kazuhiko Matsui
  • Patent number: 7833762
    Abstract: An L-amino acid is produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the acetyl-CoA synthetase activity is increased.
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: November 16, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Saori Kataoka, Takuji Ueda, Yuji Joe, Chie Koseki
  • Publication number: 20100285540
    Abstract: A process for producing (R)-?-arylalanines is described. The process converts (S)-?-arylalanine to (R)-?-arylalanine using phenylalanine aminomutase and amino acid racemase. The (R)-?-arylalanine product can be used to form pharmaceutically useful drugs. Also disclosed are DNA sequences encloding phenylalanine aminomutase and amino acid racemase enzymes, sequences for PCR primers of the DNA sequences, and sequences for expressed aminomutase and amino acid racemase enzymes.
    Type: Application
    Filed: April 30, 2010
    Publication date: November 11, 2010
    Applicant: Board of Trustees of Michigan State University
    Inventors: Kevin D. Walker, Brad M. Cox
  • Patent number: 7829657
    Abstract: Disclosed is a composition for the delivery of nucleic acid to target cells or tissues, which comprises a polycationically charged polymer as a carrier of nucleic acid. The polycationically charged polymer is a polymer which may comprise a charged polymer segment having a main chain based on poly(amino acid), polysaccharide, polyester, polyether, polyurethane or vinyl polymer and having, as a side chain, a group of formula —NH—(CH2)a—(NH(CH2)2)e—NH2 (wherein a and e independently denote an integer of 1 to 5) which is connected to the main chain either directly or via a linker. The disclosed composition has low toxicity, and has a high efficiency in introducing nucleic acid into cells.
    Type: Grant
    Filed: February 8, 2006
    Date of Patent: November 9, 2010
    Assignee: The University of Tokyo
    Inventors: Kazunori Kataoka, Keiji Itaka, Nobuhiro Nishiyama, Shigeto Fukushima, Woo-Dong Jang, Kanjiro Miyata, Masataka Nakanishi, Shunsaku Asano, Naoki Kanayama
  • Publication number: 20100279362
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.
    Type: Application
    Filed: January 30, 2008
    Publication date: November 4, 2010
    Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
  • Patent number: 7824891
    Abstract: The invention relates to an improved method for producing D-pantothenic acid and/or salts thereof and to the use thereof as an additive for animal feed.
    Type: Grant
    Filed: February 20, 2002
    Date of Patent: November 2, 2010
    Assignee: Basf SE
    Inventors: Kai-Uwe Baldenius, Christine Beck, Andreas Fischer, Hans-Peter Harz, Markus Lohscheidt, Martin Leemann
  • Publication number: 20100273221
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Application
    Filed: July 12, 2010
    Publication date: October 28, 2010
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Patent number: 7820415
    Abstract: The present invention provides a method for producing an aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ydiN gene, the ydiB gene, or both.
    Type: Grant
    Filed: February 17, 2009
    Date of Patent: October 26, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Andrey Yurievich Gulevich, Tatyana Aleksandrovna Gaydenko, Vladimir Yurievich Ermishev, Aleksandra Yurievna Skorokhodova, Irina Vladimirovna Biryukova, Sergei Vladimirovich Mashko
  • Patent number: 7811798
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to have glycerol kinase in which feedback inhibition by fructose-1,6-bisphosphate is desensitized, thereby having enhanced ability to utilize glycerol.
    Type: Grant
    Filed: June 22, 2009
    Date of Patent: October 12, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Marina Evgenievna Sheremet'eva, Yulia Aleksandrovna Ovodova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative
  • Publication number: 20100255544
    Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: September 3, 2009
    Publication date: October 7, 2010
    Applicant: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
  • Publication number: 20100255548
    Abstract: A D-aminotransferase can be modified so as to efficiently produce (2R,4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R,4R)-monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.
    Type: Application
    Filed: April 12, 2010
    Publication date: October 7, 2010
    Applicant: Ajinomoto Co., Inc.
    Inventors: Masakazu SUGIYAMA, Kunihiko Watanabe, Tatsuki Kashiwagi, Ei-ichiro Suzuki
  • Patent number: 7807420
    Abstract: The invention relates to a method for recovering a basic amino acid from the fermentation liquor of a micro-organism strain that produces the basic amino acid. The method comprises the following steps: a) isolation of the micro-organisms from the fermentation liquor and b) separation of the basic amino acid from the aqueous liquor that has been obtained in step a) by the successive charging of a single or multiple stage arrangement of a strongly acidic cation exchanger in the form of a salt with the liquor obtained in step a) and the elution of the basic amino acid. According to the invention, prior to the charging process in step b), the aqueous liquor has a pH value ranging between 4 and 7.5 and at least the first stage of the carbon exchanger arrangement is pre-treated with an aqueous acid in such a way that at the end of said pre-treatment, the pH value at the discharge of the pre-treated cation exchanger ranges between 4.5 and 7.
    Type: Grant
    Filed: April 13, 2006
    Date of Patent: October 5, 2010
    Assignee: Paik Kwang Industrial Co., Ltd.
    Inventors: Duck-Keun Han, Jong-Kyu Choi, Il-Kwon Hong, Hyun-Ho Kim, Jong-Soo Choi, Tae-Hui Kim, Sung Hyun Kim
  • Patent number: 7803584
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of a gene coding for sRNA.
    Type: Grant
    Filed: September 18, 2008
    Date of Patent: September 28, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Takuji Ueda
  • Patent number: 7794988
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rspAB operon.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: September 14, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
  • Patent number: 7794987
    Abstract: The present invention concerns the use of compounds for treating central neuropathic pain.
    Type: Grant
    Filed: December 2, 2004
    Date of Patent: September 14, 2010
    Assignee: UCB Pharma GmbH
    Inventor: Thomas Stoehr
  • Patent number: 7790422
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Grant
    Filed: April 9, 2007
    Date of Patent: September 7, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Publication number: 20100221793
    Abstract: Disclosed is a method for producing a ?-amino acid comprising a step of synthesizing a ?-amino acid from an ?-amino acid in the presence of an amino acid aminomutase. In this method, a ?-amino acid is precipitated as a solid in the reaction solution.
    Type: Application
    Filed: September 30, 2008
    Publication date: September 2, 2010
    Applicant: MITSUI CHEMICALS, INC.
    Inventor: Tomonori Hidesaki
  • Publication number: 20100221792
    Abstract: An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which is able to produce the L-amino acid, and is modified so that the activity of ribonuclease G is decreased in a medium containing glycerol as the carbon source, and collecting the L-amino acid from the culture.
    Type: Application
    Filed: March 30, 2010
    Publication date: September 2, 2010
    Inventors: YURI NAGAI, YOSHIHIRO USUDA
  • Patent number: 7781192
    Abstract: The invention relates to an improved method for producing D-pantothenic acid and/or the salts thereof and the use of said substance as an additive for animal food.
    Type: Grant
    Filed: February 20, 2002
    Date of Patent: August 24, 2010
    Assignee: BASF SE
    Inventors: Kai-Uwe Baldenius, Christine Beck, Hans-Peter Harz, Markus Lohscheidt, Daniela Klein
  • Publication number: 20100209977
    Abstract: A bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an amino acid such as L-cysteine and has been modified to have specific mutation in the yeas gene, is cultured in a medium, and the L-amino acid is collected from the medium.
    Type: Application
    Filed: February 16, 2010
    Publication date: August 19, 2010
    Inventors: Kazuhiro Takumi, Gen Nonaka
  • Patent number: 7776570
    Abstract: A recombinant microorganism is produced by introducing a DNA encoding an enzyme which hydrolyzes an amido bond of L-amino acid amide, especially L-2-alkylcysteine amide, and L-amino acid is produced by using cells or cell processed product of the obtained microorganism.
    Type: Grant
    Filed: July 17, 2008
    Date of Patent: August 17, 2010
    Assignee: Mitsubishi Gas Chemical Company, Inc.
    Inventors: Yasuhisa Asano, Atsushi Inoue
  • Patent number: 7771976
    Abstract: The present invention provides a method for producing a non-aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the csrA gene.
    Type: Grant
    Filed: July 31, 2007
    Date of Patent: August 10, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Andrey Yurievich Gulevich, Danila Vadimovich Zimenkov, Elena Vitalievna Klyachko, Tatyana Viktorovna Leonova, Yury Ivanovich Koslov, Vitaly Grigorievich Paraskevov, legal representative
  • Publication number: 20100196959
    Abstract: The invention relates to mutants and alleles of the opcA gene of coryneform bacteria, which encode variants of the OpcA subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.
    Type: Application
    Filed: January 29, 2010
    Publication date: August 5, 2010
    Inventors: Natalie Schischka, Brigitte Bathe, Georg Thierbach
  • Publication number: 20100190653
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 29, 2010
    Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jorn Kalinowski, Alfred Puhler
  • Publication number: 20100184162
    Abstract: A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a medium to produce and accumulate the L-amino acid in the medium. The microorganism has been modified by introduction of a DNA fragment which includes a pho regulon promoter and a structural gene encoding an L-amino acid biosynthetic enzyme, which is ligated downstream of the promoter so that the gene is expressed by the promoter, and so that the activity of the L-amino acid biosynthetic enzyme is increased by the expression of the gene by the promoter. In this way, the L-amino acid that is produced in the medium can be collected. Furthermore, the phosphorus concentration in the medium is such that the expression of the gene by the promoter is induced.
    Type: Application
    Filed: August 1, 2008
    Publication date: July 22, 2010
    Inventors: Akira Imaizumi, Larisa Gotlibovna Airikh, Vera Georgievna Doroshenko, Irina Sergeevna Tsyrenzhapova
  • Patent number: 7759094
    Abstract: The invention relates to a method for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the microorganisms producing the desired L-amino acid and wherein the lamB-gene or nucleotide sequence coding for the gene product maltoporin is amplified, particularly overexpressed, are cultivated in a medium in conditions enabling the desired L-amino acid to be enriched in the medium or in cells, and b) the desired L-amino acid is isolated, wherein constituents of the fermentation broth and/or biomass remain in the entirety thereof or in parts thereof (=0 bis 100%) in the isolated product or are fully removed.
    Type: Grant
    Filed: July 5, 2005
    Date of Patent: July 20, 2010
    Assignee: Degussa GmbH
    Inventor: Mechthild Rieping
  • Publication number: 20100173356
    Abstract: The present invention relates to newly identified mRNA stabilizing elements useful for the production of a target fermentation product, such as e.g. vitamins or enzymes, in particular riboflavin (vitamin B2), biotin, pantothenic acid (vitamin B5), folic acid, thiamin, pyridoxine (vitamin B6), vitamin B12, xylanase, amylase, protease, glucanase, amylomaltase or maltogenic amylase.
    Type: Application
    Filed: June 9, 2008
    Publication date: July 8, 2010
    Inventors: Martin Lehmann, Zoltan Pragai, Michèle Schaber
  • Publication number: 20100173367
    Abstract: The present invention provides functionalized molecules comprising a covalently linked autosilification moiety; and methods for making and using the functionalized molecules. The present invention provides nucleic acids comprising nucleotide sequence encoding polypeptides comprising an autosilification moiety. The present invention further provides silica matrices comprising a subject functionalized molecule, as well as systems and kits comprising the silica matrices. The subject functionalized molecules find use in various applications, which are also provided.
    Type: Application
    Filed: October 26, 2007
    Publication date: July 8, 2010
    Inventors: Wesley D. Marner, II, Afshan S. Shaikh, Jay D. Keasling, William J. Holtz, Sydnor T. Withers, III, Jeffery L. Kizer
  • Patent number: 7749742
    Abstract: A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.
    Type: Grant
    Filed: November 15, 2007
    Date of Patent: July 6, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Kenzo Yokozeki, Sonoko Suzuki, Seiichi Hara, Isao Abe
  • Patent number: 7749738
    Abstract: The present invention relates to process for increased yield of the amino acid arginine from bacterial cultures by employing strains that have been genetically manipulated for both increased arginine biosynthesis and increased level of the Escherichia coli protein YggA or a protein that is substantially similar to Escherichia coli YggA. Two strains of this invention have been deposited at MTCC, Chandigarh. The strains are GJ4894/pHYD952 (MTCC 5127) and GJ4536/pHYD953 (MTCC 5128).
    Type: Grant
    Filed: December 20, 2004
    Date of Patent: July 6, 2010
    Assignee: Centre for DNA Fingerprinting and Diagnostics
    Inventors: Gowrishankar Jayaraman, Madhusudan R. Nandineni
  • Patent number: 7745182
    Abstract: A method for producing ?-hydroxy amino acid and its optically-active isomer is provided. The ?-hydroxy amino acid is produced by reacting a predetermined D-?-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: June 29, 2010
    Assignee: Ajinomoto Co., Inc.
    Inventors: Hiroyuki Nozaki, Shinji Kuroda, Kunihiko Watanabe, Kenzo Yokozeki
  • Publication number: 20100159523
    Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: September 23, 2009
    Publication date: June 24, 2010
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: BRIGITTE BATHE, CAROLINE KREUTZER, BETTINA MOCKEL, GEORG THIERBACH
  • Publication number: 20100159536
    Abstract: The present invention relates to methods for degrading or converting a cellulosic material, methods for producing a fermentation product, and methods of fermenting a cellulosic material with an enzyme composition comprising one or more (several) cellulolytic enzymes, a cellobiose dehydrogenase, and a polypeptide having cellulolytic enhancing activity.
    Type: Application
    Filed: December 17, 2009
    Publication date: June 24, 2010
    Applicant: Novozymes, Inc.
    Inventors: Matthew D. Sweeney, Elena Vlasenko, Eric Abbate
  • Publication number: 20100151449
    Abstract: The invention relates to a method for production of L-amino acids by fermentation. According to the invention, the activity of the alanine transaminase is ether reduced or inhibited, whereby in particular the amino acids L-valine, L-lysine and L-isoleucine are produced with increased yield. Furthermore, the nucleic acids according to seq. No. 1 from position 101 to 1414 are identified as the sequence coding for the alanine transaminase gene. Use of the above permits the production of L-alanine.
    Type: Application
    Filed: April 20, 2006
    Publication date: June 17, 2010
    Inventors: Jan Marinhagen, Lothar Eggeling, Hermann Sahm
  • Publication number: 20100143981
    Abstract: The present invention relates to a whole cell catalytic system for the preparation of an enantiomerically enriched ?-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase and hydantoin racemase are coexpressed in a recombinant micro-organism wherein the genes coding for these three enzymes are located on a single replicon. The present invention further relates to the use of such a whole cell catalytic system according in the preparation of an enantiomerically enriched L-?-amino acid from a corresponding hydantoin.
    Type: Application
    Filed: December 4, 2007
    Publication date: June 10, 2010
    Inventors: Monika Rusnak-Müller, Oliver May, Petrus Johannes Hermsen, Henricus Martinus Maria Gerardus Straatman, Wolfgang Skranc, Wilhelmus Hubertus Joseph Boesten, Dannis Heemskerk, Ben De Lange, Georgios Sarakinos
  • Patent number: 7727749
    Abstract: The present invention is directed to a regio- and stereoselective bioconversion of selected aliphatic dinitriles into corresponding cyanocarboxylic acids. More particularly, the present invention provides methods for the conversion of 2-isobutyl-succinonitrile into (S)-3 cyano-5-methylhexanoic acid, which is a useful intermediate in the synthesis of (S)-3(aminomethyl)-5-methylhexanoic acid (pregabalin). Pregabalin can be used for treating certain cerebral diseases, for example, in the treatment and prevention of seizure disorders, pain, and psychotic disorders.
    Type: Grant
    Filed: April 1, 2005
    Date of Patent: June 1, 2010
    Assignee: Pfizer, Inc.
    Inventors: Michael P. Burns, Justin K. Weaver, John Wing Wong
  • Patent number: 7727748
    Abstract: The invention relates to an improved method for producing D-pantothenic acid and/or the salts thereof and to the use thereof as an additive for animal feed.
    Type: Grant
    Filed: February 20, 2002
    Date of Patent: June 1, 2010
    Assignee: BASF SE
    Inventors: Christine Beck, Hans-Peter Harz, Daniela Klein, Martin Leemann, Markus Lohscheidt
  • Patent number: 7723076
    Abstract: Methods of detecting interactions of a putative ligand with a selectively labeled target molecule, methods of screening for compounds which bind to a selectively labeled target molecule, methods for calculating the dissociation constant of a ligand that binds to a selectively labeled target molecule, and methods to determine the specific amino acids of a target molecule affected by the binding of a ligand, as well as compounds identified by these screening methods, are provided.
    Type: Grant
    Filed: August 14, 2006
    Date of Patent: May 25, 2010
    Assignee: The Burnham Institute
    Inventor: Maurizio Pellecchia
  • Publication number: 20100124769
    Abstract: The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material.
    Type: Application
    Filed: November 4, 2009
    Publication date: May 20, 2010
    Applicant: NOVOZYMES, INC.
    Inventors: Kimberly Brown, Eric Abbate
  • Publication number: 20100124583
    Abstract: Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids.
    Type: Application
    Filed: April 3, 2009
    Publication date: May 20, 2010
    Applicant: XYLECO, INC.
    Inventor: Marshall Medoff