Solid Support And Method Of Culturing Cells On Said Solid Support Patents (Class 435/395)
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Publication number: 20150093428Abstract: A 3D in vitro bi-phasic cartilage-bone organoid includes a layer of an artificial cartilage tissue, and a layer of an artificial bone tissue comprising a structure-giving scaffold and a bone marrow structure. The layer of the artificial cartilage tissue contacts at least one surface of the layer of the artificial bone tissue.Type: ApplicationFiled: February 28, 2013Publication date: April 2, 2015Applicant: TISSUSE GMBHInventors: Mark Rosowski, Shirin Fatehi-Varkani, Roland Lauster, Uwe Marx
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Publication number: 20150094808Abstract: A synthetic scaffold for replacing at least a portion of an airway includes an airway mold, one or more structural ribs on the airway mold, and a non-structural wall. Each of the one or more structural ribs is formed from a first material and the non-structural wall is formed from a second material. The non-structural wall coats the airway mold and forms a conduit that incorporates the one or more structural ribs.Type: ApplicationFiled: June 11, 2014Publication date: April 2, 2015Applicant: HARVARD APPARATUS REGENERATIVE TECHNOLOGY, INCInventor: Paolo Macchiarini
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Patent number: 8993324Abstract: In a process for the cultivation of living cells, in which the cells are cultivated on a support structure (14), the support structure (14) comprises cellulose. A process for the production of a support structure (14) of cellulose for the cultivation of living cells comprises the steps: preparation of a hollow mould; cultivation of cellulose-forming organisms in an interior space formed by the hollow mould, in order to allow the support structure (14) to grow in the interior space; demoulding of the hollow mould. In the step of demoulding the hollow mould, at least part (2, 3, 4) of the hollow mould is irreversibly deformed.Type: GrantFiled: February 12, 2008Date of Patent: March 31, 2015Assignee: Bioregeneration GmbHInventor: Günter Bertholdt
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Patent number: 8986678Abstract: The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.Type: GrantFiled: July 10, 2012Date of Patent: March 24, 2015Assignee: Centre Hospitalier Universitaire VaudoisInventor: Lee Ann Laurent-Applegate
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Patent number: 8980628Abstract: This invention relates to methods of producing hair folliclesin vitro, compositions for producing hair follicles in vitro, in vitro produced hair follicles, methods of providing an in vitro produced hair shaft at an interfollicular or intrafollicular site, methods of treating hair loss by providing an in vitro produced hair shaft at an interfollicular or intrafollicular site and assays for studying the effect of test agents on hair biology. The invention also provides the similar methods and products which are, or use, immature follicles (“defined herein as proto-hairs”). The invention provides a method for in vitro production of a hair follicle or a proto-hair comprising co-culturing dermal papilla cells with keratinocytes, and optionally with melanocytes.Type: GrantFiled: March 15, 2007Date of Patent: March 17, 2015Assignee: Aderans Research Institute, Inc.Inventors: Jizeng Qiao, Jeffrey Keeler Teumer, Erica Jean Philips
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Patent number: 8975073Abstract: A microfluidic device includes, in one embodiment, a first silk film coupled to a second silk film with at least one microchannel therebetween.Type: GrantFiled: November 21, 2007Date of Patent: March 10, 2015Assignees: The Charles Stark Draper Laboratory, Inc., Trustees of Tufts CollegeInventors: Jeffrey T. Borenstein, Chris Bettinger, David Kaplan
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Patent number: 8975074Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: October 15, 2007Date of Patent: March 10, 2015Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8975075Abstract: A hemostatic device, method of making, and method of using for internal and external applications to wounds in the body of a patient to induce hemostasis at an anatomical site.Type: GrantFiled: October 7, 2014Date of Patent: March 10, 2015Assignee: ACell, Inc.Inventors: Clay Fette, Abram Janis, Benjamin Kibalo
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Publication number: 20150064146Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: ApplicationFiled: November 6, 2014Publication date: March 5, 2015Inventors: Ed Harlow, Roderick A. Hyde, Edward K.Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, JR.
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Patent number: 8962316Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: June 12, 2006Date of Patent: February 24, 2015Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Roderick A. Hyde, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8962324Abstract: A composition for reconstruction, replacement or repair of damaged or diseased biological tissue comprising an extracellular matrix (ECM) composition that includes an ECM scaffold component and a bioactive agent component. In a preferred embodiment, the ECM scaffold component comprises mesothelial tissue and the bioactive agent comprises a statin.Type: GrantFiled: June 13, 2013Date of Patent: February 24, 2015Inventor: Robert G Matheny
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Publication number: 20150050735Abstract: Well plates for use in various laboratory experiments may include a plurality of wells each having an interior wall that includes a ledge positioned at a fixed depth within the well.Type: ApplicationFiled: August 14, 2014Publication date: February 19, 2015Inventors: Solomon Iyasere, Meagan Lazor, Eun Seok Gil, Manav Mehta
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Publication number: 20150050736Abstract: A hybrid tissue scaffold is provided which comprises a porous primary scaffold having a plurality of pores and a porous secondary scaffold having a plurality of pores, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold. The pores of the porous primary scaffold may have a pore size in a range of 0.50 mm to 5.0 mm, and the pores of the porous secondary scaffold may have a pore size in a range of 50 ?m to 600 ?m. The primary scaffold may provide 5% to 30% of a volume of the hybrid scaffold.Type: ApplicationFiled: August 25, 2014Publication date: February 19, 2015Inventors: Jeffrey N. HARRIS, Jian LING, Xingguo CHENG
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Patent number: 8956868Abstract: The present invention provides a method for producing iPS cells, comprising reacting cells with at least one connexin inhibitor and at least one TGF? signaling inhibitor; iPS cells comprising at least one connexin inhibitor; an iPS cell inducer comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors; a medium for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors; and a kit for inducing iPS cells, comprising at least one inhibitor selected from the group consisting of connexin inhibitors and TGF? signaling inhibitors.Type: GrantFiled: December 27, 2011Date of Patent: February 17, 2015Assignee: LSIP, LLCInventors: Tetsuro Takamatsu, Ping Dai
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Patent number: 8956867Abstract: A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape.Type: GrantFiled: November 9, 2009Date of Patent: February 17, 2015Assignee: Wisconsin Alumni Research FoundationInventors: Timothy J. Kamp, Jianhua Zhang, Jeffrey C. Mohr, Juan J. Depablo, Sean P. Palecek
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Publication number: 20150037385Abstract: Ink formulations comprising bioactive particles, methods of printing the inks into three-dimensional (3D) structures, and methods of making the inks are provided. Also provided are objects, such as tissue growth scaffolds and artificial bone, made from the inks, methods of forming the objects using 3D printing techniques, and method for growing tissue on the tissue growth scaffolds. The inks comprise a plurality of bioactive ceramic particles, a biocompatible polymer binder, optionally at least one bioactive factor, and a solvent.Type: ApplicationFiled: August 2, 2014Publication date: February 5, 2015Inventors: Ramille N. Shah, Adam E. Jakus
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Patent number: 8945909Abstract: The invention relates to tunable elastomeric nanochannels for nanofluidic manipulation. In particular, the present invention relates to nanochannels for performing biological assays.Type: GrantFiled: April 23, 2008Date of Patent: February 3, 2015Assignee: The Regents of the University of MichiganInventors: Shuichi Takayama, Michael David Thouless, Dongeun Huh, Kristen L. Mills, Nicholas Joseph Douville
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Patent number: 8945535Abstract: For the repair of a cartilage defect in a human or animal patient use is made of an implant comprising an implant body including a natural cartilage matrix and being coated with cells having a chondrogenic potential. These cells are produced by in vitro cell proliferation starting from chondrocytes isolated from a cartilage biopsy. The chondrocytes which are de-differentiated during cell proliferation are re-differentiated during tissue culturing and are in particular suitable for producing and maintaining the cartilage matrix of the implant body. The cells adhering to the surface of the implant body are preferably also chondrocytes being de-differentiated by cell proliferation, but not re-differentiated, and are therefore particularly suitable for integrating the implant in the defect. Due to the cells adhering to the surface of the implant body, the implant is successfully integrated in the viable tissue surrounding the defect.Type: GrantFiled: September 18, 2006Date of Patent: February 3, 2015Assignee: Zimmer Orthobiologics, Inc.Inventors: Matthias R. Steinwachs, Peter Bittmann
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Patent number: 8940478Abstract: Methods for forming cell arrays of multiple cell samples arranged substantially in a monolayer on a single substrate particularly suited for diagnostic analysis are disclosed. The cell arrays are formed with a high-speed dispensing apparatus capable of dispensing small volumes in precise, complex patterns. Also disclosed are substrates upon which cell arrays may be formed, and methods for conducting diagnostic analyzes on the formed cell arrays.Type: GrantFiled: December 3, 2012Date of Patent: January 27, 2015Assignees: Accupath Diagnostic Laboratories, Inc., Biodot, Inc.Inventors: Mathew Moore, Miriam Reyes, David Baunoch, Thomas C. Tisone, Brendan O'Farrell
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Patent number: 8927284Abstract: A method for producing a tooth having a desired length in one direction includes the steps of: placing a first cell aggregate and a second cell aggregate in the inside of a support while bringing the first and the second cell aggregates into close contact with each other; and culturing the first and the second cell aggregates in the inside of the support, in which the first cell aggregate is composed of one of mesenchymal cells or epithelial cells and the second cell aggregate is composed of the other, and the size of the tooth is controlled by adjusting the length of contact between the first cell aggregate and the second cell aggregate in one direction.Type: GrantFiled: January 14, 2010Date of Patent: January 6, 2015Assignee: Organ Technologies, Inc.Inventors: Takashi Tsuji, Kazuhisa Nakao
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Patent number: 8927282Abstract: The present invention relates to cell and tissue culture. In particular, the present invention provides a method for preparing an organotypic culture using dissociated cells or microexplants obtained from an animal organ. The method for preparing an organotypic culture comprises culturing cells from an organ on a surface characterized in that the cells are compacted. The invention further relates to a high-throughput method for the preparation of a collection of organotypic cultures. The invention further relates to a device for carrying out a method of organotypic culture according to the invention.Type: GrantFiled: June 15, 2006Date of Patent: January 6, 2015Assignee: Capsant Neurotechnologies S.A.Inventor: Luc Stoppini
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Patent number: 8927283Abstract: Methods for treating surfaces of polymeric substrates (as used in medical implants) with inert plasmas to promote the growth of bioentities (such as cells) on these surfaces is disclosed. The treated surfaces are subsequently exposed to an environment to form functionalities associated with enhanced growth of the bioentity on the surface. For example, the substrate may be exposed to the ambient environment. The bioentity may then be deposited on the modified surface. This inert plasma treatment and exposure to a suitable environment does not degrade the implants, and thus improved implants are created. Also, due to the specific functional groups at the modified surface, high cell densities are achieved.Type: GrantFiled: November 20, 2007Date of Patent: January 6, 2015Assignee: The Regents of the University of CaliforniaInventors: Kyriakos Komvopoulos, Satomi Tajima
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Patent number: 8920813Abstract: The invention relates to a replication-deficient adenoviral vector comprising two or more nucleic acid sequences encoding Dengue virus antigens and a chimeric hexon protein. The chimeric hexon protein comprises a first portion and a second portion. The first portion comprises at least 10 contiguous amino acid residues from a first adenovirus serotype (e.g., serotype 5 adenovirus hexon protein), optionally with one amino acid substitution. The second portion comprises (a) at least one hypervariable region (HVR) of a hexon protein of an adenovirus of a second adenovirus serotype, or (b) at least one synthetic hypervariable region (HVR) that is not present in the hexon protein of the wild-type adenovirus of the first adenovirus serotype.Type: GrantFiled: December 20, 2011Date of Patent: December 30, 2014Assignee: GenVec, Inc.Inventors: Joseph T. Bruder, Duncan McVey, Douglas E. Brough
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Patent number: 8921103Abstract: Compositions and methods for creating a laminar construct for tissue-engineered dermal equivalent are provided. One composition provided herein comprises a hydrogel matrix comprising two or more hydrogels layers and a population of stem cells. Associated methods are also provided.Type: GrantFiled: August 27, 2010Date of Patent: December 30, 2014Assignee: Board of Regents, The University of Texas SystemInventors: Laura Suggs, Shanmugasundaram Natesan, Ge Zhang, Robert J. Christy, Thomas Walters
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Patent number: 8921109Abstract: A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.Type: GrantFiled: September 19, 2006Date of Patent: December 30, 2014Assignee: Histogenics CorporationInventors: R. Lane Smith, Laurence J. B. Tarrant, Akihiko Kusanagi, Hans Peter Ingemar Claesson
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Patent number: 8906685Abstract: The present disclosure relates general to devices, systems, and methods of using such devices in creating and handling hanging drops of fluid. The present disclosure also relates to cell culture devices, methods and/or systems of using such devices as well as the use of cell culture devices, for example, for research and high throughput screening.Type: GrantFiled: January 28, 2011Date of Patent: December 9, 2014Assignees: The Regents of The University of Michigan, 3D Biomatrix, Inc.Inventors: Shuichi Takayama, Yi-Chung Tung, Amy Yu-Ching Hsiao, Edward Jan
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Patent number: 8906684Abstract: Provided are methods of the production of patterned 3-dimensional biopolymer scaffolds containing living cells. The methods include selective photopolymerization of biopolymers to create patterned structures and the patterning of cells within relatively homogenous slabs of biopolymer using dielectrophoresis. Also provided are patterned 3-dimensional biopolymer scaffolds generated by the methods and their use.Type: GrantFiled: January 12, 2005Date of Patent: December 9, 2014Assignee: The Regents of the University of CaliforniaInventors: Sangeeta N. Bhatia, Valerie Liu Tsang, Dirk R. Albrecht
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Patent number: 8906687Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: June 12, 2006Date of Patent: December 9, 2014Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Roderick A. Hyde, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8906686Abstract: Neo-cartilage constructs suitable for implantation into a joint cartilage lesion in situ and a method for repair and restoration of function of injured, traumatized, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected.Type: GrantFiled: April 28, 2006Date of Patent: December 9, 2014Assignee: Histogenics CorporationInventors: Shuichi Mizuno, Akihiko Kusanagi, Laurence J. B. Tarrant, Toshimasa Tokuno, Robert Lane Smith
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Patent number: 8906444Abstract: A method for modifying silk polymer by coupling a chemical moiety to a tyrosine residue of a silk polymer is described herein for the purpose of altering the physical properties of the silk protein. Thus, silk proteins with desired physical properties can be produced by the methods described herein. These methods are particularly useful when the introduction of cells to a mammal is desired, since modifications to the silk protein affect the physical properties and thus the adhesion, metabolic activity and cell morphology of the desired cells. The silk protein can be modified to produce, or modify, a structure that provides an optimal environment for the desired cells.Type: GrantFiled: May 24, 2012Date of Patent: December 9, 2014Assignee: Trustees of Tufts CollegeInventors: David L. Kaplan, Amanda Murphy
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Publication number: 20140356950Abstract: A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith.Type: ApplicationFiled: August 19, 2014Publication date: December 4, 2014Inventors: Michal AMIT, Joseph Itskovitz-Eldor
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Patent number: 8900868Abstract: A medium for growing vascular lineage cells is described. The vascular lineage cell growth medium includes an oligosaccharide-based hydrogel and a growth factor that promotes vascularization by vascular lineage cells.Type: GrantFiled: November 10, 2010Date of Patent: December 2, 2014Assignee: The Johns Hopkins UniversityInventors: Donny Hanjaya-Putra, Sharon Gerecht
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Patent number: 8900866Abstract: A method for forming a nerve graft includes the following steps. A carbon nanotube structure is provided. A hydrophilic layer is formed on a surface of the carbon nanotube structure. The hydrophilic layer is polarized to form a polar surface on the hydrophilic layer. A number of neurons are formed on the polar surface of the hydrophilic layer to form a nerve network. The neurons connect with each other.Type: GrantFiled: January 13, 2012Date of Patent: December 2, 2014Assignees: Tsinghua University, Hon Hai Precision Industry Co., Ltd.Inventors: Chen Feng, Li Fan, Wen-Mei Zhao
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Patent number: 8900851Abstract: The present invention relates to the diagnosis and treatment of cancer, and in particular breast cancer. Specifically, in some embodiments the invention relates to methods of diagnosing cancer, and in particular breast cancer, using an antibody specific for a gene product that localizes selectively to the endoplasmic reticulum of the cancer cell(s). In some embodiments, the invention relates to methods of treating cancer, and in particular breast cancer, by administering a composition comprising an RNA interference sequence (e.g., shRNA, RNAi and/or siRNA molecule) characterized by an ability to inhibit an mRNA molecule, which mRNA molecule is encoded by the C43 gene (SEQ ID NO: 1). The invention additionally relates to methods for detecting cancer cells by detecting reduced methylation of the C43 promoter, and methods for reducing cancer metastasis by using demethylation inhibitors that result in increased methylation of the C43 promoter.Type: GrantFiled: July 20, 2009Date of Patent: December 2, 2014Assignee: The Research Foundation for the State University of New YorkInventor: Jian Cao
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Patent number: 8900865Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.Type: GrantFiled: March 24, 2006Date of Patent: December 2, 2014Assignee: The Invention Science Fund I, LLCInventors: Ed Harlow, Roderick A. Hyde, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
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Patent number: 8900867Abstract: A method for forming a culture medium includes the following steps. A carbon nanotube structure is provided. A hydrophilic layer is formed on a surface of the carbon nanotube structure. The hydrophilic layer is polarized to form a polar surface on the hydrophilic layer. A number of neurons are formed on the polar surface of the hydrophilic layer.Type: GrantFiled: January 13, 2012Date of Patent: December 2, 2014Assignees: Tsinghua University, Hon Hai Precision Industry Co., Ltd.Inventors: Chen Feng, Li Fan, Wen-Mei Zhao
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Patent number: 8895304Abstract: A hemostatic device, method of making, and method of using for internal and external applications to wounds in the body of a patient to induce hemostasis at an anatomical site.Type: GrantFiled: December 6, 2012Date of Patent: November 25, 2014Assignee: ACell, Inc.Inventors: Clay Fette, Abram Janis, Benjamin Kibalo
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Patent number: 8895046Abstract: Adult autologous stem cells cultured on a porous, three-dimensional tissue scaffold-implant for bone regeneration by the use of a hyaluronan and/or dexamethasone to accelerate bone healing alone or in combination with recombinant growth factors or transfected osteogenic genes. The scaffold-implant may be machined into a custom-shaped three-dimensional cell culture system for support of cell growth, reservoir for peptides, recombinant growth factors, cytokines and antineoplastic drugs in the presence of a hyaluronan and/or dexamethasone alone or in combination with growth factors or transfected osteogenic genes, to be assembled ex vivo in a tissue incubator for implantation into bone tissue.Type: GrantFiled: October 9, 2007Date of Patent: November 25, 2014Assignee: Zimmer, Inc.Inventors: Zou Xuenong, Haisheng Li, Cody Bunger
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Patent number: 8895048Abstract: Substrates and devices for culturing cells are disclosed, along with methods of using the same. The substrates and devices include top surfaces with one or more divots disposed therein. Each divot is defined by an opening in the top surface, a rounded bottom surface spaced from the opening, and an interior side-wall surface extending between the rounded bottom surface and the opening. The top surface of the substrates and devices are optionally walled to form wells containing one or more divots. The substrates and devices may be used for reaggregating cells, for example, to form small islet cell clusters and for high throughput testing methodologies.Type: GrantFiled: July 26, 2012Date of Patent: November 25, 2014Assignee: The University of KansasInventors: Lisa A. Stehno-Bittel, Karthik Ramachandran
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Patent number: 8889414Abstract: Methods for determining metabolic properties of living cells through the uptake of semiconductor nanocrystals by cells. Generally the methods require a layer of neutral or hydrophilic semiconductor nanocrystals and a layer of cells seeded onto a culture surface and changes in the layer of semiconductor nanocrystals are detected. The observed changes made to the layer of semiconductor nanocrystals can be correlated to such metabolic properties as metastatic potential, cell motility or migration.Type: GrantFiled: October 30, 2002Date of Patent: November 18, 2014Assignee: The Regents of the University of CaliforniaInventors: A. Paul Alivisatos, Carolyn A. Larabell, Wolfgang J. Parak, Mark Le Gros, Rosanne Boudreau
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Patent number: 8889416Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device.Type: GrantFiled: January 20, 2011Date of Patent: November 18, 2014Assignees: California Institute of Technology, University of Southern CaliforniaInventors: Emil P. Kartalov, Darryl Shibata, Clive Taylor, Lawrence A. Wade
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Patent number: 8889415Abstract: A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.Type: GrantFiled: April 30, 2007Date of Patent: November 18, 2014Inventor: Ray Jui-Fang Tsai
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Publication number: 20140335612Abstract: The present disclosure provides tissue supports and methods for preparing a cartilage composition for repairing cartilage defects, which is prepared by expanding and integrating small cartilage tissue pieces derived from donor or engineered tissue. The methods and supports described herein promote cell migration and integration of neighboring tissue pieces in culture to form the cartilage composition. Methods of cartilage repair using the cartilage composition are also described.Type: ApplicationFiled: June 9, 2014Publication date: November 13, 2014Inventors: Jian Q. Yao, Hali Wang
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Patent number: 8883503Abstract: Disclosed herein are biodegradable hydrogel scaffolds for use in tissue engineering. The hydrogel scaffolds are composed of synthetic terpolymers complexed with polyvinyl alcohol (PVA), which facilitate cell-sheet and tissue growth. In the presence of a monosaccharide, the PVA-hydrogel is dissolved and cell-sheets are released for harvesting. Further disclosed herein are methods for producing PVA hydrogels which support tissue growth. Tissue engineering applications and methods are also disclosed.Type: GrantFiled: August 8, 2011Date of Patent: November 11, 2014Assignee: Indian Institute of Technology KanpurInventor: Ashok Kumar
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Patent number: 8877500Abstract: The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels.Type: GrantFiled: January 29, 2014Date of Patent: November 4, 2014Assignee: MedTrain Technologies, LLCInventors: Albert J. Banes, Mari Tsuzaki, Jie Qi
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Patent number: 8877498Abstract: The present invention relates to scaffolds that can physically guide cells, e.g. neurons, while best matching the material properties of native tissue. The present invention also relates to methods of generating such scaffolds, and for the use of such scaffolds, e.g. in spinal cord and peripheral nerve injury repair. The methods of the present invention include a uniquely controlled freeze casting process to generate highly porous, linearly oriented scaffolds. The scaffolds of the present invention not only comprise a highly aligned porosity, but also contain secondary guidance structures in the form of ridges running parallel to the pores to create a series of microstructured and highly aligned channels. This hierarchy of structural guidance aligns and guides neurite outgrowth down the channels created by the ridges, and keep neurites from branching perpendicular to the inter-ridge grooves.Type: GrantFiled: December 1, 2011Date of Patent: November 4, 2014Assignee: Drexel UniversityInventors: Ulrike G.K. Wegst, Margaret Wheatley, Benjamin W. Riblett, Nicola Francis, Amalie Elizabeth Donius
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Patent number: 8877493Abstract: The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4×104 to 10×104 cells/cm2 onto a culture substrate coated with human laminin ?5?1?1 E8 fragment or human laminin ?3?3?2 E8 fragment preferably at a concentration of 0.5 to 25 ?g/cm2, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.Type: GrantFiled: October 7, 2010Date of Patent: November 4, 2014Assignees: Osaka University, Kyoto UniversityInventors: Kiyotoshi Sekiguchi, Sugiko Futaki, Yukimasa Taniguchi, Maria Hayashi, Norio Nakatsuji, Takamichi Miyazaki, Eihachiro Kawase, Hirofumi Suemori
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Patent number: 8871462Abstract: The present invention relates to organotypic cultures of epidermal cells and the use thereof for the screening of pharmaceutical and cosmetic agents. Specifically, means for the improvement of the long-term stability of such cultures are disclosed. Thus, the present invention contemplates a skin equivalent comprising (a) a dermal equivalent comprising a matrix comprising nonwoven viscose fabric and fibroblasts and (b) keratinocytes. Moreover, the present invention contemplates a method for manufacturing the skin equivalent and a method for screening agents capable of influencing skin, such as a therapeutic or cosmetic agent.Type: GrantFiled: March 22, 2011Date of Patent: October 28, 2014Assignees: Deutsches Krebsforschungszentrum, Landesstiftung Baden-Wurttemberg gGmbHInventors: Hans-Juergen Stark, Norbert Fusenig, Petra Boukamp, Karsten Boehnke
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Patent number: 8871461Abstract: Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis.Type: GrantFiled: October 11, 2010Date of Patent: October 28, 2014Assignee: Hemoshear, LLCInventors: Brett R. Blackman, Brian R. Wamhoff
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Patent number: 8871512Abstract: Sugar-acrylic monomers are synthesized to have a carbohydrate moiety linked to an acrylate group. The sugar-acrylic monomers may be polymerized to form polymers, adhesives, hydrogels, and the like. The sugar-acrylic monomers and polymers may be used in tissue engineering, adhesives and sealers, wound healing, and the like.Type: GrantFiled: October 27, 2010Date of Patent: October 28, 2014Assignee: Empire Technology Development LLCInventors: William B. Carlson, Gregory D. Phelan, Phillip A. Sullivan