Abstract: Aspects of the present invention include probes, methods, systems that have stand alone utility and may comprise features of a drug discovery system or method. The present invention also includes pharmaceutical compositions. In more detail, the present invention provides molecular probes and methods for producing molecular probes. The present invention provides also provides systems and methods for new drug discovery. An embodiment of the present invention utilizes sets of probes of the present invention and a new approach to computational chemistry in a drug discovery method having increased focus in comparison to heretofore utilized combinatorial chemistry. The present invention also provides computer software and hardware tools useful in drug discovery systems.

Abstract: The present invention is directed to novel substrates for Hu-Asp. More particularly, the invention provides peptide substrates and fusion polypeptide substrates comprising a ?-secretase cleavage site. Methods and compositions for making and using the peptides are disclosed.

Abstract: Methods of detecting a component of interest, a change in charge, a pH, a cellular response using nanosensors are provided. Nanosensors, including nanowires and nanowire arrays comprising functionalized and/or non-functionalized nanowires are provided. Nanosensors are used for detection in cellular fragmentation, multiple concentration analysis, glucose detection, and intracellular analysis.

Abstract: The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides.

Abstract: The present invention relates to artificial receptors on scaffolds, methods of and compositions for making them, and methods of using them. Each artificial receptor includes a plurality of building block compounds coupled to a scaffold. In an embodiment, at least one of the building blocks includes a tether moiety. The tether can provide spacing or distance between the recognition element and the scaffold to which the building block is immobilized. A tether moiety can have any of a variety of characteristics or properties including flexibility, rigidity or stiffness, ability to bond to another tether moiety, and the like.

Abstract: A diagnostic tool and method of diagnosing brain injury and brain injury type (traumatic vs. ischemic) by detecting the level of expression of endothelial monocyte-activating polypeptide II (EMAP-II) and comparing to a control. An increase of EMAP-II indicates the presence of traumatic brain injury and a decrease of EMAP-II indicates the presence of ischemic brain injury.

Abstract: The present invention relates to kits and methods for the isotopic/isobaric labeling and the subsequent affinity selection and analysis of proteinaceous molecules. In particular, the invention relates to a kit-of-parts comprising a combinatorial plurality of labeling reagent for the labeling of proteinaceous molecules, each labeling reagent comprising an isobaric label component, an isotopic label component, and a reactive group capable of reacting with a proteinaceous molecule, wherein the isotopic label component concomitantly is an affinity tag. The invention is also directed to a corresponding method for the analysis of proteinaceous molecules, comprising labeling at least one subset of the proteinaceous molecules present by employing a kit-of-parts as defined herein and subsequently separating the labeled molecules by affinity purification via the affinity tag comprised in the label. Finally, the invention relates to the uses of such methods for protein expression profiling or proteomic analyses.

Abstract: A method for immobilizing a self-organizing material or fine particles on a substrate, and a substrate whereupon the self-organizing material or the fine particles are immobilized. More specifically, the method for immobilizing the fine particles including a nucleic acid (for instance, DNA or RNA) or a metal oxide on the substrate, and the substrate whereupon the nucleic acid (for example, DNA or RNA) or the metal oxide is immobilized.

Abstract: The invention provides a method of predicting the size of a dog that will be attained in adulthood, comprising typing the nucleotide(s) present for a single nucleotide polymorphic (SNP) marker present in the genome of the dog at a position equivalent to position 201 in one or more of the sequences identified in Table 1, and/or at one or more positions which are in linkage disequilibrium with any one of these positions, and thereby predicting the size of the dog that will be attained in adulthood.

Abstract: We describe assay modules (e.g., assay plates, cartridges, multi-well assay plates, reaction vessels, etc.), processes for their preparation, and method of their use for conducting assays. Reagents may be present in free form or supported on solid phases including the surfaces of compartments (e.g., chambers, channels, flow cells, wells, etc.) in the assay modules or the surface of colloids, beads, or other particulate supports. In particular, dry reagents can be incorporated into the compartments of these assay modules and reconstituted prior to their use in accordance with the assay methods. A desiccant material may be used to maintain and stabilize these reagents in a dry state.

Abstract: The present invention is directed to novel high-throughput, low-cost, and flexible solution-based methods for RNA expression profiling, including expression of microRNAs and mRNAs.

Abstract: The present invention relates to a method of diagnosis or prognosis of cardiovascular disease in a subject, said method comprising the steps of detecting the presence of activated endothelial progenitor cells (EPCs) in a sample of a circulation fluid of said subject. The invention further relates to biomarkers for diagnosis or prognosis of cardiovascular disease in a patient, said biomarker comprising the expression product of a gene the expression of which is regulated during vasculogenesis.

Abstract: There is disclosed a method for partially derivatizing a curved surface of particles in an electrically conducting solvent, said method comprises the steps: a) bringing particles in close contact with at least one surface by using a force, b) inducing a chemical reaction on at least one part of a particle by applying an electrical potential between said at least one surface and the electrically conducting solvent, and c) further reacting said at least one part of a particle where a chemical reaction has been induced in step b) above. There is further disclosed a partially derivatized particle as well as uses of the particle. Advantages include that the method is simple and only requires a potentiostat in addition to standard laboratory equipment, is inexpensive, time-efficient, and inherently parallel.

Abstract: This invention relates to methods and apparatus for detecting the pattern of post translational modification in a protein or in a plurality of proteins in a sample. One or more target proteins are subjected to predetermined proteolysis to yield plural peptide fragments comprising potential post translational modification sites. The fragments and the state of such sites are analyzed to yield a post translational pattern for the protein or proteins.

Abstract: A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.

Abstract: The invention provides a lithographic method referred to as “dip pen” nanolithography (DPN). DPN utilizes a scanning probe microscope (SPM) tip (e.g., an atomic force microscope (AFM) tip) as a “pen,” a solid-state substrate (e.g., gold) as “paper,” and molecules with a chemical affinity for the solid-state substrate as “ink.” Capillary transport of molecules from the SPM tip to the solid substrate is used in DPN to directly write patterns consisting of a relatively small collection of molecules in submicrometer dimensions, making DPN useful in the fabrication of a variety of microscale and nanoscale devices. The invention also provides substrates patterned by DPN, including submicrometer combinatorial arrays, and kits, devices and software for performing DPN. The invention further provides a method of performing AFM imaging in air.

Abstract: Capture Collections of capture compounds and collections thereof and methods using the compounds collections for the analysis of biomolecules are provided. In particular, collections, compounds and methods are provided for analyzing complex protein mixtures, such as the proteome. The compounds in the collections are multifunctional reagents that provide for the separation and isolation of biomolecules in complex protein mixtures. In the collections, each and every compound in the collection, has the formula: Z is a trityl derivative, each of m and n independently is an integer that is 1 to 100; X, the reactivity function, covalently binds to amino acid side chains of biomolecules; Y, the selectivity function, modulates binding of X to the amino acid side chains in biomolecules such that X binds to fewer biomolecules when the selectivity moiety Y is present than in its absence; and Q permits separation or immobilization of capture compounds in the collection.

Abstract: A sample processing vessel may include a branch segment and at least two tracks. The at least two tracks may be fluidly isolated from one another by a permanent seal. The tracks may be segmented by breakable seals. The branch segment may be temporarily isolated from the tracks by breakable seal(s) and put in fluid communication with the tracks once those seal(s) are broken, such that fluid received by the branch segment is divided into portions that pass into both tracks.

Abstract: The production and use of semiconducting nanopost arrays made by nanofabrication is described herein. These nanopost arrays (NAPA) provide improved laser ionization yields and controllable fragmentation with switching or modulation capabilities for mass spectrometric detection and identification of samples deposited on them.

Abstract: A method to assemble functional materials, such as nanomaterials, onto a polymer surface to create a corresponding functionalized surface involves creating a solution of the functional material, providing a sacrificial substrate, disposing the functional solution onto a surface of the substrate and then covering the substrate with a liquid polymer. The sacrificial substrate is then dissolved, leaving behind a functional surface embedded within the cured polymer. One specific aspect of the invention relates to the embedding of functionalized carbon nanotubes onto a polymer surface for creating a nano-engineered surface. Devices employing functional surfaces are disclosed that are suitable for the immobilization of enzymes, DNA, peptides, proteins, cells, catalyst, and/or other chemicals or molecules for chemical, biochemical, or biological analysis, reactions, filtration.

Abstract: Disclosed herein is a curing agent for epoxy resins that is comprised of the reaction product of an amine, an epoxy resin, and an elastomer-epoxy adduct. Additionally disclosed is a process comprising agitating a solution of an amine, an epoxy resin, and an elastomer-epoxy adduct as a dispersant at an elevated temperature in an organic medium.

Abstract: This invention provides a measuring apparatus for microanalysis, which can be simply manufactured and can realize a number of analyses and measurements in a small analyte amount and particularly can analyze and measure a number of analytes having different concentrations and different analytes in a simultaneous and easy manner, and a measurement method of microanalysis using the apparatus. The measuring apparatus for microanalysis is characterized by comprising detection parts of m lines and n rows in communication with a micropassage for a waste solution, chambers of m lines and n rows in communication with the respective detection parts through a mixing flow passage, n first micropassages in communication with the respective line chambers through a passive valve, m second micropassages in communication with respective row chambers through a passive valve, and third micropassages in communication with the respective chambers for supplying gas and/or a washing solution.

Abstract: It is an object of the present invention to provide a method for stably and efficiently binding a compound to magnetic beads. The present invention relates to a method for producing compound-bound magnetic beads, which comprises: allowing a compound to come into contact with magnetic beads, on the surface of each of which a photoreactive compound has bound; extending the magnetic beads together with the compound on a support; and applying light to the magnetic beads to form a covalent bond between the photoreactive compound and the compound.

Abstract: This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

Abstract: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20?) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20?) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and microporous, ultra-thin substrate layers (20?) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode.

Abstract: The present invention provides methods and compositions for detecting early stage ovarian cancer in a patient. Also, methods for evaluating the ovarian cancer state of a patient are described herein. These methods involve the detection, analysis, and classification of biomarkers in biological samples.

Abstract: Provided are methods, kits and arrays for carrying out relative measurement of an analyte of interest in a biological sample. As specifically exemplified, there is an array of stabilized, desiccated cDNA preparations, each at a defined location within the array, where those cDNAs were prepared from cells treated with a particular condition believed to modulate at least one gene of interest. Detection can be via Real Time Polymerase Chain Reaction using an appropriate reaction mixture and primers specific for a coding sequence of interest, and a greater relative amount of a RT PCR product from a control preparation reflects greater gene expression in response to the test condition whereas a lower amount of RT PCR product reflects an inhibitory effect on expression of the coding sequence of interest as a result of the application of the test condition.

Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.

Abstract: The present invention provides compositions, systems, and methods for identifying a patient suffering from and/or susceptible to autoimmune disease who might be likely to respond to treatment with CXCL12 and/or CXCR4 antagonists. The present invention provides novel CXCL12 and/or CXCR4 antagonists, methods of identifying novel CXCL12 and/or CXCR4 antagonists, and methods involving the use of these in the treatment of autoimmune disease.

Abstract: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.

Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for selectively converting photogenerated reagent precursors to photogenerated reagents comprising a substrate comprising at least one solid surface containing a plurality of isolated reaction sites; and an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein the irradiation pattern selectively irradiates a plurality of reaction sites without use of a photolithographic mask. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.

Abstract: The present invention relates to a process for detecting one or more analytes in one or more samples of biological origin having complex composition. The present invention also relates to a microarray for quantitative determination of one or more analytes in samples of biological origin having complex composition which are immobilized in measurement ranges of microarray, and also to a quantitative detection method based thereon.

Abstract: A combinatory analytical strip including a substrate is disclosed. A first channel for a biochemical assay and a second channel for an immunological assay are provided concavely on an upper surface of the substrate. The results of both assays are detected by a sensor. Each channel includes a first area for receiving a fluid sample, a second area for delivering the fluid sample and a third area where the fluid sample reacts. These three areas are connected successively. A nitrocellulose layer having a hollow-matrix conformation is formed at a bottom of each of the second and third areas of both channels. Each of the nitrocellulose layers of the second areas comprises an average thickness that is not greater than that of each the nitrocellulose layers of the third areas. A reaction material is formed in the hollow-matrix conformation. The third areas of the first and second channels are both located on a line conforming a relative motion path of the sensor and the combinatory analytical strip.

Abstract: This invention provides novel nanofiber enhanced surface area substrates and structures comprising such substrates, as well as methods and uses for such substrates.

Abstract: The present invention relates to the use of genes differentially expressed in benign thyroid lesions and malignant thyroid lesions for the diagnosis and staging of thyroid cancer.

Abstract: BioMEMS/NEMS appliance biologically monitors an individual, using biosensors to detect cellular components. Data is simulated or analyzed using systems-biology software, which provides diagnostic or therapeutic guidance.

Abstract: A micro-patterned plate composed of an array of releasable elements surrounded by a gel or solid wall and methods of manufacture of the micropatterned plate. The surface properties of walls can be tailored if needed to be repellent or attracting to proteins. The walls can also inhibit cell attachment. The walls enable cells or other materials to be localized to the tops of the releasable elements. The individual element in the array of releasable elements can be released from the array by a mechanical force.

Abstract: The present invention is related to a nucleic acid, preferably binding to MCP-1, selected from the group comprising type 1A nucleic acids, type 1B nucleic acids, type 2 nucleic acids, type 3 nucleic acids, type 4 nucleic acids and nucleic acids having a nucleic acid sequence according to any of SEQ. ID. No. 87 to 115.

Abstract: The present invention relates generally to the field of diagnostic and detection assays. More particularly, the present invention provides methods and reagents including biochips for detecting the presence of, or distinguishing between, one or more analytes in a sample.

Abstract: The invention relates to a method for precipitating mono or multiple layers of a mixture of organophosphoric acids of the general formula I (A) Y—B—OPO3H2 ??(IA) or of organophosphonic acids of the general formula I (B) Y—B—PO3H2 ??(IB) and the salts thereof, wherein Y and B are defined in the specification. According to the invention, precipitation occurs on substrate surfaces of pure or mixed oxides, nitrides or carbides of metals and semiconductors, in particular as surfaces of sensor platforms, implants and medical accessory devices. The invention also relates to the use thereof as part of coated sensor platforms, implants and medical accessory devices.

Abstract: Provided herein are sets of mass labels. Each mass label in a set includes: 1) a mass marker moiety; 2) a mass normalization moiety; and 3) a cleavable linker connecting the mass marker moiety to the mass normalization moiety. Each mass marker moiety is characterized as having a mass different from that of all other mass marker moieties in the set as determined by mass spectrometry. Further, each mass normalization moiety ensures that each mass label in the set has substantially the same mass as determined by mass spectrometry.

Abstract: A system and methods are provided for a medical diagnostic system that incorporates a genetically encoded digital signature to authenticate a patient. In an illustrative implementation, a platform is provided performing one or more functions including but not limited to patient authentication, diagnostics, transmission, and storage of data in a centralized secure database capable of being accessed by healthcare professionals. In the illustrative implementation, the exemplary medical diagnostic system can comprise a genetic material collector/analyzer. Responsive to inputting genetic material in the collector/analyzer, the collector/analyzer generates a unique genetic-based electronic signature representative of the genetic material. The unique genetic-base electronic signature can then be processed by cooperating parties to authenticate the person providing the genetic sample.

Abstract: A kit for providing a contrast enhancer in a mammal for contrasting during imaging of the mammal comprises functionalized gold nanoparticles configured to be directed to one or more of a target organ, tissue and lesion of the mammal.

Abstract: A rapid immunoassay method for the detection of anti-Hepatitis A Virus (HAV) neutralizing antibodies is described herein. This microplate-based enzymatic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of candidate immunogenic compositions, such as HAV vaccines, or in quantifying functional neutralizing antibodies during the course of HAV infection.

Abstract: The invention relates to a method for detection of a disease-specific combinatorial molecular pattern motif in a tissue sample of a patient's skin and/or a tissue sample of skin-neighboured-mucosa, wherein the spatial arrangement of epitopes in the sample is captured for identifying the disease-specific combinatorial molecular pattern motif. The invention moreover relates to compositions and kits comprising antibodies and/or ligands which can be employed for the performance of the method.

Abstract: Provided herein are sets of mass labels. Each mass label in a set includes: 1) a mass marker moiety; 2) a mass normalisation moiety; and 3) a cleavable linker connecting the mass marker moiety to the mass normalisation moiety. Each mass marker moiety is characterized as having a mass different from that of all other mass marker moieties in the set as determined by mass spectrometry. Further, each mass normalization moiety ensures that each mass label in the set has substantially the same mass as determined by mass spectrometry.

Abstract: The device of testing interaction between biomolecules comprising a biomolecule microarray in which a biomolecule is immobilized on a substrate and a transparent electrode (opposite electrode) positioned so as to face the surface of the substrate of the microarray on which the bimolecule is immobilized. The device comprises a nonconductive spacer between the microarray and the opposite electrode, and a cavity is formed by the microarray, spacer and opposite electrode, and the microarray comprises a conductive material surface on at least a portion of the surface on which the biomolecule is immobilized, as well as comprises two through-holes communicating with the cavity, one of which is a hole for introducing a solution into the cavity, and the other of which is a hole for discharging a solution from the cavity.

Abstract: A biomolecular assay includes a substrate with a metallic layer on at least one surface thereof. The metallic film includes nanocavities. The nanocavities are configured to enhance signals that are representative of the presence or amount of one or more analytes in a sample or sample solution, and may be configured to enhance the signal by a factor of about two or more or by a factor of about three or more. Such signal enhancement may be achieved with nanocavities that are organized in an array, randomly positioned nanocavities, or nanocavities that are surrounded by increased surface area features, such as corrugation or patterning, or nanocavities that have quadrilateral or triangular shapes with tailored edge lengths, or with a plurality of nanoparticles. Methods for fabricating biomolecular substrates and assay techniques in which such biomolecular substrates are used are also disclosed.

Abstract: Methods of amplifying nucleic acid on a solid support are described. Beads and template, each in known concentrations, are employed so a range of template to bead ratios can be exploited. Where the beads contain primers, the template can be amplified. After amplification, non-covalently bound template is removed, so as to leave beads with extended primers (or beads with primers that were not extended).

Abstract: The present invention is directed to novel substrates for Hu-Asp. More particularly, the invention provides peptide substrates and fusion polypeptide substrates comprising a ?-secretase cleavage site. Methods and compositions for making and using the peptides are disclosed.