Abstract: Provided is a method for separating two or more analytes in a fluid, which method comprises: (a) binding each different analyte to a different particle in a binding zone, to produce two or more bound analytes; (b) allowing the bound analytes to move through a separating conduit to two or more separate functional conduits; wherein each different particle has, or can be controlled to have, a different buoyancy in the fluid as compared with the other particles; and wherein the separating conduit is in fluid communication with the two or more functional conduits, each functional conduit being situated at a different height from the other functional conduits; and each functional conduit containing a fluid having a different fluid density from the fluids in the other functional conduits such that each different particle when attached to an analyte has neutral buoyancy in at least one of the functional conduits, thereby allowing separation of the bound analytes by means of the neutral buoyancies of the different

Abstract: The present invention discloses a biochip and the forming method thereof. The disclosed biochip comprises a substrate and a divalent metal compound layer on the substrate. The method for forming a biochip comprises two major steps. The first step is providing a substrate, and the second step is forming a divalent metal compound layer on the substrate.

Abstract: A combinatorial library includes a gelcast substrate defining a plurality of cavities in at least one surface thereof; and a plurality of gelcast test materials in the cavities, at least two of the test materials differing from the substrate in at least one compositional characteristic, the two test materials differing from each other in at least one compositional characteristic.

Abstract: Microarray devices fabricated using microfluidic reagent distribution techniques are provided. As described herein, the invention encompasses microfluidic microarray assemblies (MMA) and subassemblies and methods for their manufacture and use. In one embodiment first and second channel plates are provided which may be sealed to a test chip in consecutive steps. Each channel plate includes microfluidic channels configured in a predetermined reagent distribution pattern. For example, the first channel plate may have a radial (linear) reagent distribution pattern and the second channel plate may have a spiral (curved) reagent distribution pattern, or vice versa. In one embodiment the first channel plate is connected to the test chip and at least one first reagent is distributed on the test chip in a first predetermined reagent pattern. The first reagent is then immobilized on the test chip.

Abstract: The present invention relates to 1,4-disubstituted naphthalene scaffold compounds and other closely related scaffold compounds. The present invention also relates to combinatorial libraries of such compounds. In addition, the present invention relates to a method of identifying a protein-protein interaction antagonist. The method first involves providing a compound as described herein. Next, the compound is contacted with interacting proteins of a protein-protein interaction target complex, whereby the compound is allowed to compete with the interacting proteins. Then, the activity of the compound for inhibiting formation of the protein-protein interaction target complex is measured. Finally, the compound that inhibits formation of the protein-protein interaction target complex is identified as a protein-protein interaction antagonist. Also disclosed is a method for modulating a protein-protein interaction.

Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.

Abstract: A method and device are presented for modifying parameters of a solid material. This is implemented by applying radiation, such as photon flux and/or charged particle beam and/or heat, to at least a region of the material, and controlling at least one parameter of the applied radiation, thereby modifying a wettability property of the material within the irradiated region(s) thereof in a reversible manner.

Abstract: Provided is a set of mass labels, each mass label in the set comprising a mass marker moiety attached via a cleavable linker to a mass normalisation moiety, each mass label in the set having a common mass; wherein the set comprises a plurality of groups of mass labels, the mass of the mass marker moiety being the same for mass labels within a group, the mass of the mass marker moiety being different between groups; the mass marker moiety is capable of fragmentation into two or three fragments; and the mass of at least one fragment of the mass marker moiety differs between mass labels within a group.

Abstract: The present invention relates to novel coordination complexes, methods for synthesizing and identifying coordination complexes, using combinatorial techniques, and assaying their activity.

Abstract: Control beads are disclosed that allow for improved quantitation of analytes in multiplexed bead assays. The control beads have a range of concentrations of calibration moieties that provide for the preparation of a titration curve. The titration curve can be used to quantify the concentration of the analytes. The titration curve can be used to correlate the signal obtained from a bead with the concentration (or absolute number of molecules) of the analyte bound to the bead.

Abstract: The present invention aims to improve detecting accuracy and reproducibility of a biomolecule sensor. The biomolecule sensor of the present invention includes single probe molecules orderly aligned and fixed on grid points on the surface of a substrate. Accordingly, in the biomolecule sensor of the present invention: probe molecules for detecting a biomolecule are orderly aligned and separately fixed; blocking for preventing non-specific adsorption is applied to a region other than the region of the probe molecules for detecting a biomolecule; and fluorescence enhancement is achieved by metal microparticles.

Abstract: A biomolecule detection reagent comprising a semiconductor nanoparticle aggregate, wherein each semiconductor nanoparticle, constituting the semiconductor nanoparticle aggregate, has detection molecules binding specifically with biomolecules on its surface, and a standard deviation of numbers of the detection molecules existing on each semiconductor nanoparticle, is 5% or less.

Abstract: A biochip is formed with a plurality of optically clear hydrogel cells attached to the top surface of a solid substrate in the form of an array. Each of the cells is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities are immobilized in these cells, which entities are effective to selectively hybridize to or sequester a target biomolecule. Different binding entities are immobilized in different cells in an array to create a biochip that can be used to assay for a number of target biomolecules.

Abstract: A microarray with optically recorded information and a sample capable of producing a signal as a response to external influence, or a precursor which, when activated or combined with a reagent, produces a sample capable of generating a signal. The microarray is compatible with a dual functionality optical drive. A method for acquiring information about a sample comprises directing a probe to a sample at a microarray to produce a signal from the sample, wherein the microarray is compatible with a dual functionality optical drive, and detecting the signal. The information optically recorded on the microarray can be in the CD, DVD or HD DVD or Blue Ray format.

Abstract: A microdeposition pin having a contact surface with at least one concave edge for creating microarrays and the like. The microdeposition pin may be used either alone or with a plurality of microdeposition pins in conjunction with a holder. The concave edge of the pin is especially adapted for helping to control the spreading of a deposited material. By selectively controlling the spread of the reagent composition from the microdeposition pin, the flow of the reagent composition from the deposition target area may be reduced. Sensor strips having raised substrate features with limited or no spreading of the reagent composition beyond the target area are disclosed.

Abstract: A molecule for use in a method of treatment or diagnosis of Kawasaki Disease in an individual, the molecule comprising: (a) CACNA2D3; (b) CAMK2D; (c) KCNIP4; (d) ANGPT1; (e) NAALADL2; (f) ZFHX3; (g) MPHOSPH10; (h) a sequence having at least 90% sequence identity to any of (a) to (g); or (i) a modulator of any of (a) to (e). We also describe the use of Pregabalin ((3S)-3-(aminomethyl)-5-methyl-hexanoic acid) in the treatment or prevention of Kawasaki Disease in an individual.

Abstract: The invention provides a microcapsule array comprising a plurality of microcapsules immobilised on a surface, optionally in microwells in said surface. Each of the microcapsules comprises an outer layer or shell defining a microcapsule interior, said outer layer having a permeability towards a nanoscale species which is dependent on an environmental condition to which said array is exposed.

Abstract: This invention embodies a method that can be used to increase the porosity of the gel substrate during the fabrication of microchips. This increase in gel porosity will allow larger molecules such as non-fragmented RNAs and DNA fragments greater then 100 base pairs in length, and proteins to be immobilized and introduced within the gel microchips.

Abstract: The invention relates to sensor compositions comprising a composite array of individual arrays, to allow for simultaneous processing of a number of samples. The invention further provides methods of making and using the composite arrays. The invention further provides a hybridization chamber for use with a composite array.

Abstract: The present invention provides biochips that include: (a) a plurality of cards, each card having a plurality of card apertures extending therethrough, each respective card aperture having one or more cross sectional areas; and (b) a plurality of gaskets, at least one gasket residing intermediate two neighboring cards, each gasket having a plurality of gasket apertures extending therethrough. At least some of the gasket apertures have an area that is greater than that of at least one adjacent card aperture. Different sets of the gasket apertures and card apertures define a plurality of fluidic flow channels. Also provided herein are methods of making and using biochips.

Abstract: A bead immobilization method including receiving at least one bead in at least one well in a casting surface, and casting a casting material over the casting surface to form a reverse casting in which the at least one bead is cast onto at least one post upstanding from a surface of the reverse casting.

Abstract: The present invention relates to novel nucleic acid ligands or aptamers that bind to and inhibit the activation of the ?-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) subtype of ionotropic glutamate receptors. Also disclosed is a novel combination of technologies, i.e., SELEX and laser pulse photolysis for the selection and screening of aptamers that inhibit receptor function and are useful therefore, in the treatment of diseases associated with excessive activation of ionotropic glutamate receptors.

Abstract: The present invention relates to novel methodologies for performing multiplexed assays for biological molecules such as proteins and nucleic acids. In particular, the present invention provides multiplexed assays using precipitating reagents and optically clear nitrocellulose-coated solid supports.

Abstract: BioMEMS/NEMS appliance biologically monitors an individual, using biosensors to detect cellular components. Data is simulated or analyzed using systems-biology software, which provides diagnostic or therapeutic guidance.

Abstract: The present invention relates to methods for identifying compounds capable of modulating a cellular response. The methods involve attaching living cells to solid supports comprising a library of test compounds. The test compounds are linked to the solid support via cleavable linkers and may thus be released from the solid supports. Solid supports comprising cells, wherein the cellular response of interest has been modulated are selected and the test compound of the solid support can then be identified. The cellular response may for example be changes in complex formation between proteins.

Abstract: The present invention relates to a method for screening a sol composition for sol-gel biochips, which is used to immobilize a probe on a surface-untreated substrate, also relates to a sol composition screened by said method and a sol-gel biochip comprising said sol composition immobilized thereon. The sol composition screened by the disclosed method can be mixed with a probe, and the sol mixture can be integrated on 96-well plates, which are widely used in existing bioassays, without surface treatment. Also, the biochip can provide a sensitive and specific good analysis results because this immobilization methods of probe maintain the nature of probes without modification.

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

Abstract: The present invention relates to artificial receptors, arrays or microarrays of artificial receptors or candidate artificial receptors, methods of and compositions for making them, and methods of using them. Each artificial receptor includes a plurality of building block compounds. In an embodiment, at least one of the building blocks includes a tether moiety. The tether can provide spacing or distance between the recognition element and the support or scaffold to which the building block is immobilized. A tether moiety can have any of a variety of characteristics or properties including flexibility, rigidity or stiffness, ability to bond to another tether moiety, and the like.

Abstract: The invention relates to processes and devices for the controlled fabrication of nanostructures from starting components that have high fidelity recognition properties and multiple binding groups. In one embodiment, the invention relates to the formation of nanostructures using controlled sequential addition of nanocomponents at regular intervials via sequential formation of binding pairs or other chemical binding reactions.

Abstract: Parallel, integrated bioreactor. The bioreactor includes a plurality of growth chambers, each growth chamber associated with a peristaltic oxygenating mixer and separated therefrom by a porous membrane. Each oxygenating mixture has a gas inlet and a gas outlet with the gas inlet in fluid communication with a gas reservoir. A gas mixer switch is provided for controlling oxygen concentration in the reservoir. The apparatus and methods disclosed in the application allow precise control over dissolved oxygen concentration with a quick response time.

Abstract: The invention relates to a method and device for obtaining micro and nanometric particles in a controlled, reproducible manner. The aforementioned particles have a spherical shape and a very narrow, uniform size distribution. More specifically, the invention relates to a novel method of forming emulsions and to the application thereof in micro and nanoencapsulation techniques involving the extraction/evaporation of the solvent. In particular, the invention relates to the encapsulation of the fluorescent compounds and the subsequent application thereof.

Abstract: The invention relates to a printer device for producing biological test arrays by depositing an array of biofluids onto a substrate. The invention further relates to the use of such a device in the production of biological test arrays. The invention also relates to a method for producing a biological test array. The invention moreover relates to a biological test array. The method according to the invention is failure-proof, and results in biological test arrays of superior quality.

Abstract: A Multiform Suspension Microgranular Bioreactor (MSMB) is used to detect nucleic acids or peptides/proteins in molecular and biological samples. Polymer-based microgranules, coupled with biomass molecule probes, are constructed with different features. The biomass molecule probes include cDNA probes, oligonucleic acid probes, peptide or protein probes. These microgranules are differentiated according to their features including shape, size, color, fluorescence intensity, magnetic property, gravity, chemical luminal intensity, radioactivity, and other labels. The above microgranules are suspended in a sample solution and are used collectively as a microarray-like bioreactor device. Such MSMB-based microarrays are particularly useful in complicated biological detection assays with flow cytometry.

Abstract: The present invention is directed to controlling plant pest infestation, and particularly plant nematode infestation, by inhibiting one or more biological functions in the plant pest. The invention discloses methods and compositions for use in controlling plant pest infestation by providing one or more different recombinant double stranded RNA molecules in the diet of the pest in order to achieve a reduction in pest infestation through suppression of pest gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, to methods for detecting cells comprising the disclosed sequences, and to methods for detecting the disclosed sequences in biological samples.

Abstract: Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

Abstract: Systems including apparatus, methods, compositions, and kits for multiplexed analysis of biological systems using nonpositional and/or positional arrays of coded carriers.

Abstract: The present invention relates to functionalized porous carriers which comprise a material having at least one porous surface, nanoparticles having molecule-specific recognition sites being present in the pores of the material surface, and to a process for producing functionalized porous carriers. The invention further relates to functional elements produced using the functionalized carriers, such as microtiter plates, microarrays and flow devices, and also to uses of the functionalized carriers and functional elements.

Abstract: Microparticles including spatially coded microparticles, systems for imaging and methods of detecting such microparticles as well as using the same in bioassays are provided.

Abstract: A microchannel array, a method of manufacturing the same, and a blood test method. The microchannel array is formed by joining first and second substrates, each including a fluid inlet and outlet on their surfaces. An internal space structure connects the fluid inlet and outlet, and includes an upstream flow channel connected with the fluid inlet, a downstream flow channel connected with the fluid outlet with a gap therebetween, and a micro flow channel connecting the upstream and downstream flow channels. A minimum distance from a center of a sectional surface of the micro flow channel to a side wall of the micro flow channel is smaller than that of the upstream and downstream flow channels. Each surface of the first and second substrates includes grooves for creating the upstream and downstream flow channels, and the surface of the second substrate has grooves for creating the micro flow channel.

Abstract: The invention provides a method for the structural analysis of a saccharide, comprising: a) providing on a surface a plurality of essentially sequence- and/or site-specific binding agents; b) contacting said surface with a saccharide to be analyzed, or with a mixture comprising a plurality of fragments of said saccharide; c) washing or otherwise removing unbound saccharide or saccharide fragments; d) adding to the surface obtained in step c) an essentially sequence- and/or site-specific marker, or a mixture of essentially sequence- and/or site-specific markers; e) acquiring one or more images of the markers that are bound to said surface; and f) deriving information related to the identity of the saccharide being analyzed from said image.

Abstract: The present invention provides a microarray having base cleavable, sulfonyl-containing linkers and a process to make the microarray. Oligonucleotides of any sequence may be synthesized on the microarray having the cleavable linker. The oligonucleotides may be cleaved and recovered as a pool of oligonucleotides having a three prime phosphate moiety. Specifically, the microarray is an electrode containing microarray, and the oligonucleotides are electrochemically synthesized.

Abstract: A method of fabricating a micro-electrode array comprising the steps of coating an electrode with an insulating polymer coating by screen printing an insulating polymer onto the electrode and then curing said polymer; and sonically ablating the insulating polymer coating to produce a plurality of micro-pores.

Abstract: Described herein are supports for assaying an analyte and methods of making and using thereof.

Abstract: Methods are provided for detecting and optionally quantitating multiple analytes, including nucleic acid and/or polypeptide analytes, in particle-based assays that can be highly multiplexed. Compositions, systems, and kits related to the methods are also featured.

Abstract: A microarray and a process for making the microarray having a library of chemical compounds are disclosed herein. Each member of the library is attached at a known location. The microarray has a plurality of addressable electrodes and a porous reaction layer attached to each electrode. The porous reaction layer has reactive hydroxyl groups. Michael acceptors are attached to the porous reaction layer of selected electrodes. The Michael acceptors are attached at the hydroxyl groups using electrochemically-generated base that is generated by the selected electrodes and confined to the selected electrodes with the use of excess activated ester. At least one of a plurality of chemical compounds from a library of compounds is attached to the olefin of the plurality of Michael acceptors. Attachment occurs only at the selected electrodes having Michael acceptors with unreacted olefin. Each member of the library of compounds is attached to the known locations.

Abstract: A test apparatus is for testing a DNA substrate on which a plurality of DNA fragments for testing are arranged, wherein absolute precision is not required. A substrate is provided on which a plurality of biomolecule spots containing a group of biomolecules (e.g., DNA, etc.) of a specific type are formed, where the pattern or position of the DNA spot is changed depending on specific data so that information of the specific data is recorded on the substrate.

Abstract: A binary-coupled type probe array for analyzing components of a biological sample using probes, a biochip, and a method of fabricating the same, are provided. The binary-coupled probe array can include a substrate, a plurality of first probes immobilized on a top surface of the substrate, and a plurality of second probes immobilized on a bottom surface of the substrate.

Abstract: The present invention provides a method of patterning, the method comprising the steps of: (a) providing a porous film; and (b) adding at least one structure to the porous film. The present invention also provides a patterned film prepared according to the method of the invention. The present invention also provides a method of preparing a porous film, and a porous film prepared according to the method of the invention.

Abstract: Described are mathematical models and method, e.g., computer-implemented methods, for predicting tumor sensitivity to radiation therapy, which can be used, e.g., for selecting a treatment for a subject who has a tumor.

Abstract: The present invention relates to artificial receptors and arrays or microarrays of artificial receptors or candidate artificial receptors. Each member of the array includes a plurality of building block compounds, typically immobilized in a spot on a support. The present invention also includes the building blocks, combinations of building blocks, arrays of building blocks, and receptors constructed of these building blocks together with a support. The present invention also includes methods of making and using these arrays and receptors.