Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
Type:
Grant
Filed:
December 1, 2008
Date of Patent:
September 9, 2014
Assignee:
Life Technologies Corporation
Inventors:
Jun Lee, Robert Potter, David Mandelman
Abstract: This invention relates to the use of nucleic acid sequences of the MAP kinase-interacting kinase (Mnk) gene family and amino acid sequences encoded thereby, and to using these sequences or effectors of Mnk nucleic acids or polypeptides, particularly Mnk kinase inhibitors and activators, in the diagnosis and treatment of diseases and disorders related to body-weight regulation and thermogenesis. One aspect of the disclosure encompasses methods of identifying an animal or human having an elevated probability of having or developing a pancreatic malfunction, the method comprising: (a) obtaining a biological sample from an animal or human subject; and (b) determining from the biological sample whether the subject has a genetic variant of an Mnk2 and/or Mnk1 gene or a homolog thereof, or an expression product of said Mnk2 and/or Mnk1 gene or homolog thereof, wherein said genetic variant is associated with an elevated probability of having or developing a pancreatic malfunction.
Type:
Grant
Filed:
September 26, 2011
Date of Patent:
September 9, 2014
Assignee:
Boehringer Ingelheim International GmbH
Inventors:
Arnd Steuernagel, Karsten Eulenberg, Guenter Broenner, Thomas Ciossek, Bettina Rudolph, Dorothea Rudolph, Funmi Belgore, Stefan Jaekel, Christoph Meyer
Abstract: The present invention relates to a recombinant host cell for the production of a compound of interest. The invention further relates to a method for the production of such host cell. The invention further relates to the production of a compound of interest. The invention further relates to isolated polynucleotides and vectors and host cells comprising said polynucleotides.
Type:
Application
Filed:
April 10, 2014
Publication date:
September 4, 2014
Applicant:
DSM IP ASSETS B.V.
Inventors:
Noël Nicolaas Maria Elisabeth VAN PEIJ, Herman Jan PEL, Thibaut Jose WENZEL, Adriana Marina RIEMENS, Ilse DE LANGE
Abstract: The present invention provides for compositions and methods for producing crop plants that are resistant to herbicides. In particular, the present invention provides for wheat plants, plant tissues and plant seeds that contain altered acetyl-CoA carboxylase (ACCase) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ACCase protein.
Type:
Application
Filed:
January 31, 2012
Publication date:
September 4, 2014
Applicant:
Colorado Wheat Research Foundation, Inc.
Inventors:
Michael Hal Ostlie, Scott Haley, Philip Westra, Victoria Ashley Valdez
Abstract: The present invention provides novels genes encoding methyl butenol (MBO) synthase, methy butenol synthases and their use in methyl butenol production.
Type:
Application
Filed:
February 6, 2014
Publication date:
September 4, 2014
Applicant:
Board of Trustees of Michigan State University
Abstract: The invention provides isolated NUE (nitrogen utilization efficiency) nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering nitrogen utilization and/or uptake in plants. The invention further provides recombinant expression cassettes, host cells and transgenic plants.
Type:
Grant
Filed:
September 4, 2013
Date of Patent:
September 2, 2014
Assignee:
Pioneer Hi Bred International Inc
Inventors:
Howard P Hershey, Dale F Loussaert, Carl Simmons
Abstract: Articles and methods for the delivery of drugs and/or nucleic acids. Articles including a nanoparticle are provided that may be used for the delivery of a drug, a nucleic acid, or both, to a subject. The articles may be of polymeric material and may self-assemble.
Type:
Grant
Filed:
May 19, 2004
Date of Patent:
September 2, 2014
Assignee:
Agency for Science, Technology and Research
Inventors:
Yi-Yan Yang, Yong Wang, Cherng-wen Tan, Jackie Y. Ying
Abstract: The present invention provides compositions and methods for producing a polyol oxidase in micoroorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.
Type:
Grant
Filed:
January 18, 2013
Date of Patent:
September 2, 2014
Assignee:
Danisco US Inc.
Inventors:
Manoj Kumar, Susan M. Madrid, Hugh C. McDonald, Ayrookaran J. Poulose, Thomas Rand, Huaming Wang
Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.
Type:
Application
Filed:
October 5, 2012
Publication date:
August 28, 2014
Applicant:
LONZA LTD.
Inventors:
Diethard Mattanovich, Brigitte Gasser, Michael Maurer, Roland Prielhofer, Joachim Klein, Jana Wenger
Abstract: The invention relates to a method for the ozonolysis of unsaturated fatty acids and derivatives thereof, comprising a) ozonolysis of a fatty acid or of a derivative thereof in an organic phase comprising an organic solvent, and b) contacting the organic phase with an aqueous phase comprising catalase and preferably a buffer, where the fatty acid or the derivative thereof has a linear chain having at least eight carbon atoms, and the use of catalase for removing reactive oxygen species from a reaction mixture comprising ozonolysis products of an unsaturated fatty acid.
Type:
Grant
Filed:
September 21, 2012
Date of Patent:
August 26, 2014
Assignee:
Evonik Degussa GmbH
Inventors:
Matthias Ullrich, Peter Hannen, Martin Roos
Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of co-producing coenzyme Q10 and at least one ?-3/?-6 polyunsaturated fatty acid are provided. The strains may also be engineered to co-produce at least one C40 carotenoid. Methods of using the antioxidant products obtained (e.g., biomass and/or pigmented oils) in food and feed applications are also provided.
Abstract: Compounds and methods for refolding of proteins in an aqueous solution. In particular, biocompatible multiblock copolymer surfactants such as poloxamers, meroxapols, poloxamines, or polyols are used to catalyze proper refolding without changing the protein composition, and restore the protein to its native conformation and native biological function. The methods can be practiced both in vivo and in vitro. The biocompatible multiblock copolymer surfactants can be used for renaturation of recombinantly expressed proteins, and for renaturation of proteins that are unfolded due to heat, irradiation, mechanical shearing, electrical shock, frostbite, chemical stress, and other abiotic or biotic stresses.
Abstract: The present invention provides a protein, specifically Parietochloris incisa acetohydroxyacid synthase, and methods for producing branched-chain amino acid in a cell. The present invention further provides polypeptides and polynucleotides useful for conferring herbicide resistance in a cell or an organism. In particular, the present invention discloses a mutated acetohydroxyacid synthase which is resistant to herbicides.
Type:
Application
Filed:
October 4, 2012
Publication date:
August 21, 2014
Applicant:
BEN-GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY
Inventors:
Omer Grundman, Inna Khozin-Goldberg, Zvi Hacohen, Michal Shapira, Dina Raveh, Sammy Boussiba, Boris Zorin
Abstract: The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3? hydroxyl group of RNA in the absence of ATP using the ligase.
Type:
Grant
Filed:
January 25, 2013
Date of Patent:
August 19, 2014
Assignee:
The Rockefeller University
Inventors:
Thomas Tuschl, Janos Ludwig, Yi Pei, Carolina Lin
Abstract: A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure prepared by subjecting an acid of the structure to reduce amination by treating the acid with ammonium formate, nicotinamide adenine dinucleotide, dithiothreitol and partially purified phenylalanine dehydrogenase/formate dehydrogenase enzyme concentrate (PDH/FDH) and without isolating treating the resulting amine of the structure 2 with di-tert-butyl dicarbonate to form the BOC-protected amine.
Type:
Grant
Filed:
January 4, 2013
Date of Patent:
August 19, 2014
Assignee:
AstraZeneca AB
Inventors:
Michael Politino, Matthew M. Cadin, Jason G. Chen
Abstract: Modified nucleotide molecules of xylanase and the application of the nucleotide molecules in constructing recombinant vectors, host cells or producing xylanase are disclosed, wherein the nucleotide molecules contain nucleotide sequences having greater than 80% identity with nucleotide sequence shown by SEQ ID NO: 1.
Abstract: The present invention relates to specially selected catalase enzymes and their use in reducing hydrogen peroxide in applications, and particularly in aseptic packaging applications.
Type:
Grant
Filed:
July 1, 2013
Date of Patent:
August 12, 2014
Assignee:
Ecolab USA Inc.
Inventors:
Brandon L. Herdt, Joshua P. Magnuson, David D. McSherry, Junzhong Li, Krista L. Owens
Abstract: The present invention relates to a thermophilic arabinose isomerase and a method of manufacturing tagatose using the same, and more precisely, a gene encoding arabinose isomerase originating from the thermophile Thermotoga neapolitana DSM 5068, a recombinant expression vector containing the gene, a method of preparing a food grade thermophilic arabinose isomerase from the recombinant GRAS (Generally Recognized As Safe) strain transformed with the said expression vector, and a method of preparing tagatose from galactose using the said enzyme.
Type:
Grant
Filed:
October 17, 2008
Date of Patent:
August 12, 2014
Assignee:
CJ Cheiljedang Corporation
Inventors:
Seong-bo Kim, Young-mi Lee, Seung-won Park, Jung-hoon Kim, Sang-hoon Song, Kang-pyo Lee, Hye-won Kim, Hye-jin Choi
Abstract: An isolated protein which is at least partially encoded by a polynucleotide sequence encoding a novel elongase is provided together with a composition which includes the isolated protein. A transgenic plant or a transgenic seed transformed by a polynucleotide encoding a protein which is at least partially encoded by a novel elongase is also provided. The invention also includes a process for making a very long-chain polyunsaturated fatty acid in a transformed cell or plant expressing the isolated protein which is at least partially encoded by a polynucleotide sequence encoding a novel elongase.
Type:
Grant
Filed:
November 26, 2009
Date of Patent:
August 12, 2014
Assignee:
Ben Gurion University of the Negev Research and Development Authority
Abstract: The invention provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP. Methods, kits and compositions for monitoring changes in metabolites such as ADP are disclosed.
Abstract: The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.
Type:
Grant
Filed:
November 4, 2010
Date of Patent:
August 12, 2014
Assignee:
DSM IP Assets B.V.
Inventors:
Alrik Pieter Los, Brenda Vonk, Marco Alexander Van Den Berg, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
Abstract: The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.
Type:
Application
Filed:
April 5, 2012
Publication date:
August 7, 2014
Applicant:
BASF SE
Inventors:
Lukas Maksym, Ramona Knab, Stefan Evers, Karl-Heinz Maurer, Johannes Bongaerts
Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
Type:
Application
Filed:
April 20, 2012
Publication date:
August 7, 2014
Applicant:
DANISCO US INC.
Inventors:
Elizabeth A. Bodie, Robert James Pratt, II
Abstract: Disclosed are a method for rapid screening of suitable translational fusion partners (TFPs) capable of inducing expression or secretory production of non-producible proteins, which are difficult to produce in conventional recombinant production methods, from a variety of genetic sources, and protein secretion-inducing TFPs obtained using the method.
Type:
Application
Filed:
January 30, 2014
Publication date:
August 7, 2014
Applicant:
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
Abstract: The present invention provides compositions, systems and methods for demulsifying an emulsion including an aqueous phase and an organic phase by adding an effective amount of a composition comprising at least one quaternary organopolysiloxane or salt thereof to the emulsion, the composition optionally including at least one of quaternary epihalohydrin/polyamine copolymers or salts, and/or (poly)diallyldimethylammonium halides.
Type:
Grant
Filed:
September 5, 2012
Date of Patent:
August 5, 2014
Assignees:
Kroff Chemical Company, Dow Agrosciences LLC
Inventors:
Paul Rey, David M. Sunderlik, Steven P. Ceplecha
Abstract: The present invention provides a therapeutic agent for a lower urinary tract disease such as cystitis, interstitial cystitis, prostatitis, benign prostatic hyperplasia etc., which are considered as refractory disease, and an agent for improving a lower urinary tract symptom associated with the lower urinary tract disease.
Abstract: The present invention provides a tnaluronidase. The hyaiuronidase can be produced by the strain Streptomyces aitinocidm 77, Exemplary characteristics of the hyaluronidase include specific C-terminal or other amino acid sequences, including full-length sequences, and improved physico-chemical and actix itj properties as compared to known h>aluronidase preparatkiiis. Described are also various uses of the hyaiuronidase, including topical administration of the h>aiuronidase to improve skin penetration of a co-administered active substance.
Abstract: Disclosed are methods and compositions for detection and amplification of nucleic acids, wherein two DNA strands hybridized to an RNA strand are ligated. In one aspect, the disclosed methods include removal of an energy source, such as ATP, upon charging a ligase to form an enzyme-AMP intermediate, and then adding substrate, which results in one complete round of RNA-templated DNA ligation. In another aspect, the ligation reaction is accomplished by use of a mixture of at least two different ligase enzymes. The disclosed methods and compositions for RNA-templated DNA ligation may be particularly useful for detection of RNA sequence variants, for example RNA splice variants, and for quantitative expression analysis.
Type:
Grant
Filed:
January 19, 2010
Date of Patent:
July 29, 2014
Assignee:
Affymetrix, Inc.
Inventors:
Eugeni A. Namsaraev, Xin Miao, John E. Blume
Abstract: The degree of polymerisation (DP) is an important parameter for analysis of saccharide antigens, particularly in glycoconjugates. The invention provides methods that can be used to measure DP for capsular saccharides, particularly for meningococcal saccharides e.g. from serogroups W135 and Y. A preferred method is based on reduction of terminal sialic acid residues on saccharides, with DP then being calculated by comparing the molar ratio of total sialic acid to reduced sialic acid.
Abstract: The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.
Type:
Grant
Filed:
September 2, 2011
Date of Patent:
July 29, 2014
Assignee:
DSM IP Assets B.V.
Inventors:
Aaron Adriaan Winkler, Sipko Maarten Kuyper, Wilhelmus Theordorus Antonius Maria De Laat, Johannes Pieter Van Dijken, Jacobus Thomas Pronk
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Type:
Grant
Filed:
July 31, 2012
Date of Patent:
July 29, 2014
Assignee:
Novozymes, Inc.
Inventors:
Paul Harris, Elena Vlasenko, Marcus Sakari Kauppinnen, Elizabeth Zaretsky, Sarah Teter, Kimberly Brown
Abstract: A device, system and method for producing glycosylated proteins in plant culture, particularly proteins having a high mannose glycosylation, while targeting such proteins with an ER signal and/or by-passing the Golgi. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. More particularly, the invention relates to host cells, particularly transgenic suspended carrot cells, vectors and methods for high yield expression and production of biologically active high mannose Glucocerebrosidase (GCD). The invention further provides for compositions and methods for the treatment of lysosomal storage diseases.
Abstract: The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity.
Type:
Grant
Filed:
January 30, 2012
Date of Patent:
July 29, 2014
Assignee:
DSM IP Assets B.V.
Inventors:
Jan Metske Van Der Laan, Margot Elisabeth Francoise Schooneveld-Bergmans, Denise Ilse Jacobs
Abstract: The present invention provides compositions and methods for the use of oil-containing materials as feedstocks for the production the bioproducts by biofermentation. In one preferred embodiment, surfactants are not used in compositions and the methods of the invention. In one preferred embodiment the oil-containing feedstocks are the by-products of other industrial processes including microbial, plant and animal oil processing.
Type:
Application
Filed:
January 22, 2014
Publication date:
July 24, 2014
Applicant:
Glycos Biotechnologies, Inc.
Inventors:
Daniel J. Monticello, Ryan Black, Paul Campbell
Abstract: The present invention provides improved formulations for lysosomal enzymes useful for enzyme replacement therapy. Among other things, the present invention provides formulations that preserve or enhance the stability and/or efficacy of a lysosomal enzyme such as acid alpha-glucosidase.
Abstract: A non-natural protein having at least one ester bond in its polypeptide main chain is synthesized by using an in vivo translation system in a ribosome. The following components (a) to (c) are expressed in a cell or an cell extraction solution in the presence of an ?-hydroxy acid: (a) an aminoacyl-tRNA synthetase which can activate the ?-hydroxy acid; (b) suppressor tRNA which can bind to the ?-hydroxy acid in the presence of the aminoacyl-tRNA synthetase; and (c) a gene encoding a desired protein having a nonsense mutation or a frame-shift mutation at a desired site.
Abstract: The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
Type:
Grant
Filed:
September 16, 2013
Date of Patent:
July 15, 2014
Assignee:
Commonwealth Scientific and Industrial Research Organisation
Inventors:
Surinder Pal Singh, Stanley Suresh Robert, Peter David Nichols, Susan Irene Ellis Blackburn, Xue-Rong Zhou, James Robertson Petrie, Allan Graham Green
Abstract: Compositions suitable for oral administration to an animal comprising at least one immune stress-reducing enzyme in an amount effective to decrease the level of positive acute phase protein in an animal, increase the level of negative acute phase protein in an animal, and/or improve animal growth performance is provided, as are methods using such compositions. The compositions include animal feed compositions, liquid compositions other than animal feed, and solid compositions other than animal feed.
Type:
Grant
Filed:
March 25, 2011
Date of Patent:
July 15, 2014
Assignee:
Eli Lilly and Company
Inventors:
David M. Anderson, Humg-Yu Hsiao, Lin Liu
Abstract: This invention provides for a novel means of incorporating non-native amino acids into preselected positions of a protein using a cell-free synthesis system. The methods involve the use of non-orthogonal, native isoaccepting sense tRNAs that are encoded by the genetic code. Such methods allow for numerous non-native amino acids to be incorporated through the use of sense codons without having to rely upon orthogonal tRNA-synthetase pairs.
Type:
Grant
Filed:
January 12, 2010
Date of Patent:
July 15, 2014
Assignee:
Sutro Biopharma, Inc.
Inventors:
Alexei M. Voloshin, James F. Zawada, Daniel Gold, Christopher James Murray, James Edward Rozzelle, Nathan Uter, Gang Yin
Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.
Abstract: The present invention relates to a novel method for the biocatalytic production of unsaturated dicarboxylic acids by cultivating a recombinant microorganism co-expressing a glutaconate CoA-transferase and a 2-hydroxyglutaryl-CoA dehydratase system. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyamide or polyester copolymers making use of said dicarboxylic acids as obtained by said biocatalytic production method.
Type:
Grant
Filed:
January 15, 2010
Date of Patent:
July 15, 2014
Assignee:
BASF SE
Inventors:
Oskar Zelder, Wolfgang Buckel, Ivana Djurdjevic
Abstract: Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor.
Type:
Application
Filed:
March 4, 2014
Publication date:
July 10, 2014
Applicant:
Xyleco, Inc.
Inventors:
Marshall Medoff, Thomas Craig Masterman
Abstract: The invention provides compositions and methods for ligating single stranded nucleic acids wherein the ligation is based on fast, efficient, and low-sequence bias hybridization of an acceptor molecule with a donor molecule. In one embodiment, the structure of the donor molecule comprises a stem-loop intramolecular nucleotide base pairing (i.e., hairpin) and a 3?-overhang region such that the overhang is able to hybridize to nucleotides present in the 3? end of the acceptor molecule.
Type:
Application
Filed:
January 9, 2014
Publication date:
July 10, 2014
Applicant:
The Penn State Research Foundation
Inventors:
Philip C. Bevilacqua, Sarah M. Assmann, Yiliang Ding, Chun Kit Kwok
Abstract: The present invention relates, in general, to telomeres, and, in particular, to a method or effecting telomere elongation in mammalian cells. The invention further relates to a protein suitable for use in such a method and to nucleic acid sequences encoding same.
Type:
Grant
Filed:
April 30, 2004
Date of Patent:
July 8, 2014
Assignee:
Duke University
Inventors:
Christopher M. Counter, Blaine N. Armbruster
Abstract: A process is disclosed for the biological treatment under anaerobic/anoxic conditions for the degradation of pollutants present in brine solution used in waste water management, in industrial waste brine solutions and in waste oil field brine solutions, especially waste brine solutions contaminated with perchlorate and nitrate. A culture capable of reducing perchlorate and nitrate in spent ion-exchange regenerant brine containing at least 30 g/L NaCl under anaerobic/anoxic conditions was used to demonstrate the process. A stabilized brine solution is also disclosed in which a culture capable of degrading a given pollutant can be proliferate.
Type:
Grant
Filed:
November 19, 2004
Date of Patent:
July 8, 2014
Assignee:
The University of Houston System
Inventors:
Deborah J. Roberts, Dennis A. Clifford, Xiaohua Lin, Thomas Gillogly, Lee Aldridge, Stewart Lehman
Abstract: Biogas is obtained in a solid-state anaerobic digester from a solid organic biomass and a digestion effluent produced in an associated liquid anaerobic digester. The solid organic biomass and digestion effluent are mixed to produce an effluent-biomass mixture. The effluent-biomass mixture is then incubated in the solid state anaerobic digester, producing a biogas and a digestate. The incubation is controlled by adjusting the composition and properties of the effluent-biomass mixture. The solid organic biomass may comprise lignocellulosic biomass, food waste, agricultural waste and the like.
Type:
Grant
Filed:
February 14, 2012
Date of Patent:
July 8, 2014
Assignee:
The Ohio State University Research Foundation
Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
Type:
Grant
Filed:
October 12, 2011
Date of Patent:
July 8, 2014
Assignee:
Ajinomoto Co., Inc.
Inventors:
Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii
Abstract: The invention relates to recombinantly produced ?-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
Type:
Grant
Filed:
June 16, 2010
Date of Patent:
July 8, 2014
Assignee:
Codexis, Inc.
Inventors:
Xiyun Zhang, Jie Yang, Dipnath Baidyaroy, Sally Rhiannon Postlethwaite, Attila Andor, Louis Clark, Lisa M. Newman