Abstract: Provided is Sphingomonas paucimobilis SQL2 having base sequences of which a sequence number is 1, being deposited with a deposition number KCTC 1171BP, and being used for specifically measuring aromatic hydrocarbons as a contaminant in a sample.

Abstract: The subject of the present invention is a bacterial composition having immunomodulation properties comprising at least one strain selected from the group consisting of Lactobacillus acidophilus PTA-4797, Lactobacillus plantarum PTA-4799, Lactobacillus salivarius PTA-4800, Lactobacillus paracasei PTA-4798, Bifidobacterium bifidum PTA-4801 and Bifidobacterium lactis PTA-4802. An other subject of the invention is an immunomodulation method comprising the step of using the at least one strain selected from the preceding group.

Abstract: The present invention relates to embedding live or dead microorganisms and/or bioactive materials in a protective dry formulation matrix, wherein the formulation includes the bioactive microorganism or material, a formulation stabilizer agent, and a protective agent. The formulation is prepared by dispersing all the solid components in a solution, with or without a vacuum, and cooling the solution to a temperature above its freezing temperature. The methods include a primary drying step of the formulation at a desired temperature and time period, and an accelerated secondary drying step under maximum vacuum and elevated temperature, to achieve a final desirable water activity of the dry material.

Abstract: The present invention relates to pleomorphic cells (“morphotes”), which exhibit morphologic and genetic characteristics of both prokaryotic and eukaryotic cells, including resemblance to prokaryotic cells at the unicellular level, and resemblance to eukaryotic cells at the multicellular level due to their ability to self-organize in vitro into multicellular, mammalian tissue-like patterns consisting of tissue-like sheets, capillary-like networks, and trabecular (spongy) bone-like structures. Morphotes have a number of applications in the diagnostic medical, therapeutic medical, biological, biomaterials, bionanotechnological, and industrial fields.

Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.

Abstract: A synthesis procedure for benzazolo[3,2-a]quinolinium chloride salts and the inclusion of chloro-substituent, amino-substituent, and nitro-substituent resulting in several compounds wherein said procedures provides an increment in the compounds biological activity. The compounds are further used for intra cellular binding, cytotoxicity on malignant cells through apoptosis activation mediated by mitochondrial damage, cellular organelles binding and damage, DNA fragmentation, marker of bacterial growth, antibacterial activity, cell cycle disruption, and a marker due to the auto-fluorescent properties.

Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.

Abstract: This invention provides co-cultures of photosynthetic microorganisms and biofuel producing microorganisms. In certain embodiments, polysaccharide-producing, photosynthetic microorganisms are microalgae having frustules provide a substrate on which biofuel-producing microorganisms can grow. In other embodiments, the photosynthetic microorganisms produce a lipid and the non-photosynthetic microorganisms produce a solvent in which the lipid is soluble.

Abstract: Probiotic lactic bacteria of the Lactobacillus genus, isolated from Donkey milk, are disclosed, as well as their use in food or beverage compositions such as fermented milk products.

Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.

Abstract: Live attenuated bacteria vaccines are provided. Also provided are methods by which such vaccines can be obtained, including: a method by which a copy of the dam gene from a pathogenic bacteria is cloned into a plasmid capable of replication in the same bacteria species such that it is overexpressed from either a lac promoter, tac promoter, araBAD promoter, trc promoter, trp promoter, T7, SP6, or T5 bacteriophage promoters, a native promoter from that species, or other appropriate promoter. The plasmid containing the dam gene is then transferred into the pathogens to cause increased expression of Dam resulting in the formation of a live attenuated bacterial vaccines. Alternative methods for producing the vaccine are also provided including altering or replacing the chromosomal promoter for the native dam gene so as to alter Dam expression or mutating or replacing the native dam gene so as to alter the expression of Dam in a pathogenic bacteria.

Abstract: Methods and composition involving genetically engineered targeting conjugates with reversed orientation of VL and VH chains are provided. For example, in certain aspects targeting conjugates comprising VL and VH chains of anti-CD22 and anti-CD19 are described. In a further aspect, the invention provides methods and targeting conjugates comprising therapeutic agents or diagnostic agents for delivery to B cells.

Abstract: A live organism product wherein the organisms are in a dormant state and are suspended in a liquid carrier which is sufficiently devoid of moisture so that the organisms will remain in a dormant state for several months. The carrier is comprised of oil. The carrier may also include an absorbent. The product is stored and shipped in a plastic bag and is sprayed onto its target host or the like. The moisture and pH then activates the organisms.

Abstract: According to the invention there is provided a consumable component kit for use in a system which utilises a liquid culture of a microbiological material, the kit including: a sealed and aseptic culturing vessel; at least one sealed and aseptic chamber for storing a liquid nutrient medium; a sample of the microbiological material; a sample of the nutrient medium which is suitable for use in a feedstock for the microbiological material; optionally, a sample of salts and other components of the liquid nutrient medium; and a plurality of aseptic conduits for connecting the chamber to the culturing vessel, and for connecting the culturing vessel to the remainder of the system to enable a liquid supply of the microbiological material, or a product or extract thereof, to be utilised by the system.

Abstract: Disclosed is a pharmaceutical composition for inhibiting infection and replication of influenza A and B virus, and the manufacture thereof. The pharmaceutical composition is produced by a manufacture of low-temperature disintegrating cyanobacteria, comprising the steps of: (a) mixing cyanobacteria and non-organic solvent to form a suspension containing cyanobacteria; (b) freezing the suspension with a temperature below 0° C. to form a ice block and the ice block being melted at a low temperature, the whole step being repeated at least twice; (c) separating the cyanobacterial residues and extract solution of the suspension; and (d) collecting the isolated cyanobacterial extract solution; wherein the cyanobacterial extract solution is a solution containing cyanobacterial bioactive substances. The pharmaceutical composition is able to effectively inhibit binding of sialic acid and hemagglutinin of influenza A and/or B virus, so as to inhibit infection and replication of influenza virus.

Abstract: The present invention refers to a process to increase the concentration of micro organisms' colonies which are formed on the surface of Gramineas bambusoideae in a batch and/or continuous flow process which utilizes biomass as a filtration means in order to remove nitrates as well as organic and inorganic impurities from water and/or from affluents in which a step of adsorption is followed by a step of biological degradation by the anaerobic digestion of micro organisms of the types Pseudonomas SP (Nitrosomonas, Nitrosococus, Nitrobacter, Azobacter, Azotomas and Rhixobium). In accordance to the present invention, the addition of about 200-300 ppm of sodium acetate to the solution fed to the reactor, maintaining a relation of 2:1, C:N, provides an efficiency increase of 80% to 98% regarding the removal of nitrate as well as organic and inorganic soluble matter from the water.

Abstract: Device for carrying out photochemical processes on a microscale and use of the device for photochemical reactions and culturing photosynthesizing cells and/or microorganisms.

Abstract: The present invention relates to a method and kit for the isolation of cells from a sample. The sample is treated with an extraction solution that comprises at least MgCl2 and/or an ionic liquid resulting in the isolation of preferably viable cells.

Abstract: A fermented liquid feed composition for a target animal is provided, the feed composition being prepared by the fermentation of a feed substrate with a lactic acid producing bacteria, the lactic acid bacteria being characterised by: a) being viable under the conditions prevailing in the gastrointestinal tract of the target animal; b) being an aggregating bacteria and/or co-aggregating with one or more pathogens; and c) being able to produce upon fermentation in the feed substrate lactic acid in an amount of at least a minimum inhibitory concentration of lactic acid. A method of producing a fermented liquid feed composition is also provided. Preferred lactic acid producing bacteria for use in the composition and method are Lactobacillus.

Abstract: A composition, and method of use, for controlling, reducing or preventing the growth of protozoal bacteria in the rumen of a ruminant comprising cell bodies of a facultative anaerobe selected from the group consisting of Corynebacterium cell bodies and Brevibacterium cell bodies or a mixture of Corynebacterium cell bodies and Brevibacterium cell bodies, glutamic acid fermentation solubles, corn fermentation solubles, glutamic acid fermentation solubles and corn fermentation solubles.

Abstract: The present invention relates to human specific Escherichia coli strains.

Abstract: Systems and methods for capture and sequestration of CO2, SOx, NOx, and/or other compounds present in industrial waste streams and use of the captured waste as a nutrient source to support a microbial population. The microbial population can use the nutrients from the industrial waste stream and an energy source such as sunlight or a hydrocarbon deposit for the production of biomass, heavy metals recovery, and the generation of methane and other biogases. Biomass can be processed to produce a variety of hydrocarbon fuels, lubricants, and the like.

Abstract: Described are microorganisms which are able to inhibit the formation of foot malodor by skin microorganisms. Also described are compositions comprising such microorganisms as well as the use of such microorganisms in cosmetic, prophylactic or therapeutic applications.

Abstract: The subject invention provides a novel and advantageous strain of Pasteuria bacteria with nematicidal activity against lance nematodes. The subject invention provides the novel bacterial culture referred to as ATCC SD-5832, and mutants or variants thereof. Also provided are nematicidal compositions comprising the Pasteuria strain or its mutants or variants, and uses thereof for treating phytopathogenic and soil-dwelling nematodes.

Abstract: The present invention generally relates to the field of preventing and/or treating inflammatory and infectious disorders, in particular by boosting the endogenous antimicrobial defences. One embodiment of the present invention is the use of B. longum NCC2705 (deposit number CNCM I-2618) for use in the treatment or prevention of disorders related to the immune system including infections.

Abstract: The present invention relates to an in vitro and/or ex vivo method of screening probiotic bacterial strains. The present invention also relates to a method of assessing quality of probiotic culture. In addition, the present invention relates to use of animal cells in screening of a probiotic strain. The present invention also relates to use of animal cells in assessing quality of probiotic culture.

Abstract: An improved method for the design and development of high performance hybrid devices having biological and nonbiological components. The biological component is used in hybrid constructs that may be nanostructures, given the small size of the biological parts. In one specific embodiment, chlorosomes of Chloroflexus aurantiacus (C. aurantiacus) enhance performance of a silicon photovoltaic cell. C. aurantiacus, strain J-10-f1, has the A.T.C.C. designation number 29366, having been deposited in July, 1976. Its chlorosomes are harvested and positioned in light communicating relation to a photoactive semiconductor. The chlorosomes react to light of a first wavelength by emitting light at a second wavelength to which the semiconductor electrically responds.

Abstract: The present invention relates to high-throughput methods of screening biological samples to identify microorganisms having potential utilities as biocontrol agents. The methods include, for example, the use of multitest platforms for the simultaneous identification of microorganisms having biocontrol activity, including those useful in improving plant, animal, and human health. In particular, the present invention provides screening methods suitable for identification of microorganisms having potential applications in combating diseases caused by plant pathogens. The disclosure also provides microorganisms having biocontrol activity that are identified by the screening methods disclosed herein.

Abstract: The present invention relates to a mutated Francisella bacterium, wherein the ggt gene is silenced or deleted; pharmaceutical compositions comprising the same, in particular, vaccine compositions, and use of the bacterium and compositions for treatment and/or prophylaxis, and in particular, the treatment or prophylaxis of tularemia.

Abstract: This invention relates to an aeration system comprising an aeration section, a degassing section and optionally an additional section. This invention also relates to a horizontal photobioreactor comprising the aeration system. This invention further relates to methods of using the photobioreactors.

Abstract: This invention relates to Bifidobacterium breve CNCM I-3865, to a composition comprising Bifidobacterium breve CNCM I-3865 and to the use of Bifidobacterium breve CNCM I-3865 in the prevention or treatment of rotavirus diarrhoea.

Abstract: A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber.

Abstract: The present invention relates to antibodies against human collagen II, polypeptides and polynucleotides encoding human collagen II antibodies or fragments thereof, and methods of making and using the foregoing.

Abstract: Biofungicide composition derived from a biologically pure culture of a Chilean bacterial isolate obtained from the skin of grapes, corresponding to Serratia plymuthica CCGG2742, to be used as an environmentally friendly biological control agent against fungal diseases of vegetables, in particular fruits susceptible to the infection of Botrytis cinerea, efficiently preventing the germination of conidia and the proliferation of mycelia of said phytopathogenic fungus, furthermore protecting the plant's leaves and fruits from the infection by the same fungus, and having the potential of being used in the biological control of other phytopathogenic fungus and microorganisms.

Abstract: The present invention relates to the field of biotechnology or genetic engineering. More specifically, the present invention relates to a multiple inducible gene regulation system that functions within cells to simultaneously control the quantitative expression of multiple genes.

Abstract: Disclosed are: a novel microorganism which can decompose a methylthiotriazine compound (particularly, simetryn, dimethametryn, prometryn), a chlorotriazine compound (particularly, simazine, atrazine, propazine) and a methoxytriazine compound (particularly, simeton, atraton, prometon) which have been frequently used as agrichemicals or the like; and a method for decomposing a methylthiotriazine compound, a chlorotriazine compound and/or a methoxytriazine compound by using the microorganism. Specifically disclosed are: a novel bacterium Nocardioides sp. strain MTD22 which is capable of decomposing a methylthiotriazine compound, a chlorotriazine compound and a methoxytriazine compound; and a method for decomposing a methylthiotriazine compound, a chlorotriazine compound and/or a methoxytriazine compound, particularly simetryn, dimethametryn, prometryn, simazine, atrazine, propazine, simeton, atraton and/or prometon, by using the microorganism.

Abstract: The invention relates to cells and a method for producing acetone.

Abstract: The present invention provides compositions and methods that enable the stabilization and storage of samples by contacting a sample with an assembly of particles, and reducing the water activity level of the contacted sample. By reducing the water activity level of the sample, the assembly of particles minimizes the degradation of the sample. Stabilizers may or may not be added to the assembly of particles to further minimize the degradation of the sample. Subsequently to storage in the assembly of particles, the samples are recoverable by eluting the assembly of particles with a fluid solution. In one embodiment, the entire assembly of particles will dissolve into the solution. In another embodiment, only part of the assembly of particles will dissolve into the solution. The assembly of particles provides the advantage that while it is porous, it comprises non-porous particulate material.

Abstract: Different bioremediation agents' effectiveness against concentrations of diesel fuel in soil were tested. The purpose of this experiment is to find a quick, clean, and cost-effective alternative to current remediation techniques using new bioremediation techniques. Analysis indicated that the use of a mixture of Linoleic acid and R. rhodochrous generated the greatest, quickest, and longest lasting degradation rate. The mixture provided a 51.23% drop in PAH and data suggests that it will to remediate after the end of the 4th week. In second place came the Linoleic acid with an immediate drop of 36.61% before leveling out in the 2nd week. The bacteria (R. rhodochrous) started slow but moved into a greater rate every week bringing it to an overall 46.65% change. The hypothesis that the mixture of the R. rhodochrous and Linoleic acid would yield the greatest PAH degradation was supported by the data.

Abstract: A method for treatment of a material comprising lignocellulosic fibres is disclosed. More particularly, the treatment increases the accessibility of the lignocellulosic fibres for following microbial or biological processes.

Abstract: The present invention pertains to a system for culturing cells comprising a culturing bag and a continuous flow centrifuge wherein the cells are continuously separated from the supernatant and are recycled into the culturing bag. Further provided are methods for culturing cells and for producing a biological substance using the device for culturing cells, and the use of a bag for culturing cells in said device or said methods. In particular, a perfusion system for culturing cells is provided wherein the wave technology for culturing cells is combined with continuous flow centrifugation for separating the medium from the cells.

Abstract: Fermentation products (e.g., bioproducts such as hydrocarbons and other organic compounds) are produced from biomass or gases through digestion and fermentation under conditions that thermodynamically favor production of the fermentation products.

Abstract: A microorganism capable of degrading an estrogenic substance contained in wastewater and the like; a method for the simple and effective degradation of an estrogenic substance contained in domestic wastewater, wastewater discharged from a livestock farm, or the like by using the microorganism; and an apparatus for achieving the degradation are provided. An estrogenic substance-degrading microorganism which belongs to the genus Pseudaminobacter, Gordonia, Nocardia, Zoogloea, Pandoraea, Cryptococcus or Trichosporon and has an ability to degrade an estrogenic substance; a method for the degration of an estrogenic substance by using the microorganism; and an apparatus for achieving the degradation are provided.

Abstract: The present invention relates to a composition for prevention and/or treatment of human skin alteration or disease comprising an extract of marine bacteria selected from Flavobacteriaceae, wherein the extract contains an effective amount of zeaxanthin. The present invention also relates to a method for preparing the above composition, comprising: (a) culturing marine bacteria selected from Flavobacteriaceae, more specifically, from Olleya marilimosa, in a liquid culturing medium to form pigments containing zeaxanthin; (b) separating cell mass of the marine bacteria with the pigments and the liquid culturing medium and collect the cell mass to obtain the above composition, and (c) optionally mixing the cell mass and a carrier.

Abstract: The present invention relates to a method for polypeptide transfer into cells. The present invention further relates to the detection of polypeptide-specific immune cells and the priming, expansion and reactivation of polypeptide-specific T cells. Moreover the present invention relates to polypeptides of the methods of the present invention in combination with urea and their use for research, diagnosis or treatment and prevention of diseases in animals and humans.

Abstract: The present application discloses a method for pre-treating a sample of a DNA/RNA adsorbing matrix (e.g. soil) suitable for extracting cell-derived (deoxy) ribonucleic acid therefrom, comprising the steps of: isolating a sample of a DNA/RNA adsorbing matrix, said sample comprising DNA/RNA-containing cells; and bringing the sample into contact with DNA of natural origin in a fragmented form (e.g. salmon sperm DNA) so as to essentially block said DNA/RNA adsorbing binding sites of said adsorbing matrix, said fragmented DNA being “unrelated” to the target DNA/RNA of said DNA/RNA-containing cells. The fragmented DNA of natural origin can be depurinated and/or freeze-dried. The application further discloses a method for extracting DNA/RNA from a sample and a kit useful for pre-treating samples.

Abstract: The present invention provides method of identifying molecules that cooperatively and positively interact with either a ligand or a target molecule of a ligand/target molecule pair, or molecules that interact with a ligand/target molecule complex.

Abstract: The present invention relates to growing anaerobic bacteria by measuring the reduction potential of a growth media at a start time and determining a grow time by correlating the start time reduction potential with said reduction coefficient to calculate when said second anaerobic fermentation system will reach a sufficiently low oxygen concentration to enable growth for an anaerobic bacteria, then adding the anaerobic bacteria to the growth media at a time no sooner than the grow time.

Abstract: The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one or more microorganisms within a sphere of the gelating agent; (v) coating the spheres containing the entrapped microorganism(s) with a natural or synthetic polymer to form a polymeric membrane; (vi) incubating the coated spheres in the original environment for an appropriate time; (vii) cutting the spheres and scanning for microorganisms colonies; and (viii) isolating the microorganisms, and repeating steps (iii) to (vii) until a pure clone of said previously unculturable microorganism is obtained.

Abstract: Provided is a bacterial strain which produces a family of mosquitocidal toxins, Xenorhabdus MT, on deposit with the American Type Culture Collection, PTA-6826, insecticidal compositions comprising the mosquitocidal toxin(s) produced by Xenorhabdus MT, a mosquitocidal toxin preparation prepared from spent culture medium, whole culture or cells or a mixture thereof, of Xenorhabdus MT and method of insect control, especially mosquito control. Also provided are microbial compounds (same as mosquitocidal toxins) compositions comprising them and use in formulating therapeutic and other antimicrobial compositions, and methods of use for inhibiting microbial growth and for treating infection.