Abstract: An object of the present invention is to provide a more simplified and more efficient process for producing a highly purified orange roughy oil having high storage stability. The present invention provides a process for producing a highly purified orange roughy oil substantially free of a polyunsaturated fatty acid ester having 4 to 6 double bonds, and having a saponification value of 98 to 113 and an iodine value of 73 to 89, comprising washing with an alkaline aqueous solution to remove a free fatty acid; hydrogenating with a catalyst to reduce a polyunsaturated fatty acid ester; and purifying by treatment with an adsorbent.

Abstract: Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ?-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one ?9 elongase/?8 desaturase multizyme, in addition to other heterologous ?9 elongases, ?8 desaturases, ?5 desaturases, ?17 desaturases, ?12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

Abstract: A method is provided for growing microalgae from wastewater for oil production in a three-step wastewater treatment facility. In the method, two carbon sources are selected for addition to the wastewater, which contains naturally-occurring bacteria. Specifically, the first carbon source is selected to increase the carbon-to-nitrogen ratio and the carbon-to-phosphorous ratio within the microalgae. The first carbon source serves as a food source for the microalgae, and the second carbon source promotes the breakdown of carbon nitrogen and phosphorous by the bacteria cells into a more easily digestible form for the microalgae. Due to the added carbon, the wastewater supports growth of the microalgae and the production of oils therein.

Abstract: Methods are provided for pelletizing a microbial biomass, extracting a refined lipid composition from the pelletized biomass under supercritical conditions and distilling the refined lipid composition, at least once under short path distillation conditions, to obtain a lipid-containing fraction. Also disclosed are methods of making lipid-containing oil concentrates therefrom, by transesterifying and enriching the lipid-containing fraction.

Abstract: Disclosed is a process for reducing the amount of sterol in a sterol-containing microbial oil composition, including distilling, under short path distillation conditions, a sterol-containing microbial oil wherein said distillation produces a distillate fraction containing the sterol and a triacylglycerol-containing fraction having a reduced amount of the sterol when compared to the amount of sterol in the sterol-containing microbial oil composition that has not been subjected to short path distillation.

Abstract: Systems and methods for extracting lipids of varying polarities from oleaginous material.

Abstract: The present invention provides certain improvements in methods for processing vegetable oils and apparatus for carrying out such methods. One embodiment provides a method for processing a partially processed oil including glycerides and a volatilizable impurity fraction. The partially processed oil may processed by driving off a first volatiles stream comprising a portion of the glycerides and at least a portion of the volatilizable impurity fraction, leaving a deodorized oil. The first volatiles stream may be introduced into a first condensing chamber and a glyceride-rich, impurity-poor first condensate may be condensed from the first volatiles stream, leaving a glyceride-poor, impurity-rich second volatiles stream. The second volatiles stream may be passed into a second condensing chamber and a glyceride-poor, impurity-rich second condensate may be condensed from the second volatiles stream.

Abstract: A process for separation of a mixture containing a microbial substance and a liquid using deformable filter is provided.

Abstract: A protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. The heating and cooling stages are rapid, with the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least ?0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, ° C.) graph, a trapezium is obtained having an area less than 13,000° C. minute. This results in a smaller energy input and a better quality oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

Abstract: A reverse phase extraction method for the recovery of triglycerides from aqueous slurries of algae is described herein. The present invention discloses the use of immobilized anion exchange and lipid binding resins. As the algae flows past the resin, triglycerides adhere while the bulk of the algae flows through. The lipids, useful for generating biofuels are then are eluted off the resin. The method of the present invention does not require prior drying of the algae, is inexpensive, and does not destroy the algal biomass which can be used for other purposes.

Abstract: This invention relates to the design of a process by intermittent dielectric heating combined with a recycling system. This process consists in subjecting reagents to electromagnetic waves selected in the frequencies ranging between 300 GHz and 3 MHz intermittently using a recycling system. This process enables the treatment of oils that are hardly absorbent as well as great investment savings. This process enables operation on different scales, whether in laboratories, on a semi-industrial or industrial scale, without forfeiting the advantages of continuous dielectric heating.

Abstract: The present invention is directed to processes for preparing oil compositions having a high concentration of poly-unsaturated fatty acids and oil compositions having a low concentration of ?-linolenic acid. In addition, the present invention is directed to processes for preparing oil compositions having advantageous stability characteristics.

Abstract: Provided herein are methods of producing and processing a feedstock from a photosynthetic microorganism. Also provided are various transgenic photosynthetic microorganism and photobioreactors for use in the methods.

Abstract: Described are processes for obtaining fatty acids with reduced content of by-products, wherein: (a) unrefined fats or oils are subjected to steam stripping in a counter-current column to produce (i) a bottom fraction of de-acidified fats or oils, (ii) a first distillate consisting mainly of fatty acids and glycerides, and (iii) a second distillate comprising fatty acids and essentially all unwanted ketones, aldehydes and phenols; and (b) said bottom fraction (i) containing the de-acidified fats or oils and said first distillate (ii) containing fatty acids and glycerides are combined.

Abstract: A pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has a heating stage, a plateau stage at which the cells are held at a (maximum and) constant temperature, and a cooling stage. The heating and cooling stages are rapid, the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least ?0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, ° C.) graph, one obtains a trapezium having an area less than 13,000° C. minute. This results in a smaller energy input (so a reduction in costs) and a better quality (and less oxidised) oil having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

Abstract: Continuous method for the esterification of free fatty acids in plant and animal fats with alcohols using a heterogenic acid catalyst.

Abstract: A method for stabilizing retinoic acid is provided. The method includes a step of mixing retinoic acid with a hydrophobic polymer/hydrophilic polymer adduct in the presence of an oxygen containing atmosphere to form a retinoic acid containing composition. The hydrophobic polymer/hydrophilic polymer adduct comprises a poly(vinylpyrrolidone/alkylene) polymer and a polymer comprising repeating carboxylic acid groups, hydroxyl groups, or a mixture of carboxylic acid groups and hydroxyl groups. A retinoic acid containing composition and a method of using a retinoic acid containing composition are provided.

Abstract: Systems and methods for extracting lipids of varying polarities from oleaginous material.

Abstract: To provide a method for supplying lauric acid with algae. The method for producing an oil or fat containing lauric acid as a constituent fatty acid includes culturing algae in the class Chlorarachniophyceae in a medium and recovering, from the culture product, an oil or fat having a lauric acid content of 3 weight % or higher of the fatty acid composition.

Abstract: A method for producing an oil or fat containing lauric acid as a constituent fatty acid including: culturing, in a medium, at least one species of algae in the class Cryptophyceae selected from the group consisting of algae belonging to the genus Rhodomonas and algae belonging to the genus Chroomonas selected from among Chroomonas diplococca, Chroomonas mesostigmatica, Chroomonas nordstedtii, and Chroomonas placoidea and recovering, from the culture product, an oil or fat having a lauric acid content of 3 weight % or higher of the fatty acid composition.

Abstract: Natural oil-based polyols that are high in color, e.g., >40 APHA, are decolorized when exposed to high frequency visible light and/or low frequency UV light, with or without the combination of heat and/or air exposure.

Abstract: Vegetable oils having a low level of glycidol esters are disclosed. Methods for reduction of the content of glycidol esters in edible oils are also disclosed.

Abstract: The present invention is a system for harvesting algae in continuous fermentation. There is a harvester including a main moving belt, a plurality of rollers, and a motor for driving the main motor belt. There is a reactor tank and a vacuum extractor for applying a vacuum over a width of the main moving belt to extract biomass and to dry the main moving belt. The main moving belt has one end in the reactor tanks and another end extended into the vacuum extractor. The algae contained in the reactor tank is collected for further processing, including oil extraction. With algae harvested in the large-scale manner of the present invention, a more efficient oil extraction method can be used because of the concentration, temperature, and pressure can be more easily controlled.

Abstract: The processes of producing the low pour point palm diesel particularly but not exclusively via esterification of C18, C18:1 and C18:2 mixed fatty acids with methanol or ethanol, or fractional distillation of methyl or ethyl esters of palm oil, palm kernel oil and palm oil products, or fractional distillation of methyl or ethyl esters of palm oil, palm kernel oil and palm oil products, followed by crystallization, or crystallization of methyl or ethyl esters of palm oil, palm kernel oil and palm oil products, or crystallization of methyl or ethyl esters of palm oil, palm kernel oil and palm oil products, followed by fractional distillation.

Abstract: A method of making biofuels and valuable food and neutraceutical products includes dewatering intact algal cells to make an algal biomass, extracting neutral lipids along with carotenoids and chlorophylls from the algal biomass, and separating the carotenoids and chlorophylls. The remaining neutral lipids are esterified with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising omega-3 fatty acids esters and remaining carotenoids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.

Abstract: The development and application of a novel non-polar oil recovery process utilizing a non-dispersive solvent extraction method to coalesce and recover oil from a lysed or non-lysed Yeast slurry using a microporous hollow fiber (MHF) membrane contactor.

Abstract: Lipids can be extracted from a microbial biomass that constitutes at least 20% lipids by weight and has a moisture content of less than 4% by weight by applying pressure to the biomass so as to release lipids therefrom, thereby leaving a biomass of reduced lipid content; and collecting the lipids.

Abstract: A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, sequentially adding solvent sets to the algal biomass, and sequentially separating solid biomass fractions from liquid fractions to arrive at a liquid fraction comprising neutral lipids. The method also includes esterifying the neutral lipids, separating a water miscible fraction comprising glycerin from a water immiscible fraction comprising fuel esters, carotenoids, and omega-3 fatty acids. The method also includes obtaining a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C16 or longer fuel esters to obtain a diesel blend stock.

Abstract: The present invention provides novel lysophospholipid acyltransferases. The object of the present invention is attained by the nucleotide sequences of SEQ ID NOs: 1 and 6 and the amino acid sequences of SEQ ID NOs: 2 and 7 of the present invention.

Abstract: The present invention relates to a method for purification of lipid material originating from biological material. In the method the lipid material comprising acylglycerols and phosphorus impurities and at least one added nonpolar solvent and at least one added polar solvent is provided into a reaction zone whereby at least a two phase system comprising a nonpolar phase and a polar phase is formed. The phase system is heated in the closed reaction zone under mixing at a temperature from 150° C. to 300° C. and at a pressure wherein said solvents are in subcritical state, preferably of below 100 bar, dependent on the vapor pressure of the selected solvents, until the phosphorus impurity is removed from the polar phase. Sub-sequently, the nonpolar phase including the purified oil comprising acylglycerols is separated and recovered from said phase system.

Abstract: A process for producing an oil, or a polyunsaturated fatty acid (PUFA), is described where an aqueous liquid comprising cells is deaerated, and the oil or PUFA is obtained from the cells. Deaeration can be performed by a wide variety of techniques, including the application of a vacuum (or reduced pressure), mechanical deaeration or degassing by reduced stirring or subjecting the broth to centrifugal forces, reducing viscosity (by dilution or heating), reduction in the supply of oxygen or air during fermentation or a reduction in stirring rate, lowering the pH (to lower the solubility of CO2), filtration using PTFE capillaries, gas displacement (by bubbling in nitrogen or helium) or chemical deaeration (using oxygen scavengers).

Abstract: The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

Abstract: Palm oil processing plants are continually looking for ways to improve oil recovery, and reduce bio-chemical oxygen demand and chemical oxygen demand of the palm oil mill effluent. A method is described for increasing the amount of oil recovered and reducing the bio-chemical oxygen demand and chemical oxygen demand of palm oil mill effluent by treating pressed palm oil slurry with ultrasonic energy prior to disposal of the effluent.

Abstract: A method for producing biofuels along with valuable food and neutraceutical products is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids along with carotenoids and chlorophylls from the algal biomass, and separation of the carotenoids and chlorophylls using adsorption or membrane diafiltration or other methods. The remaining neutral lipids are esterified with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising omega-3 fatty acids esters and remaining carotenoids.

Abstract: The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils.

Abstract: Methods and electroporation devices for electrical treatment of algal cell cultures for release of lipids and proteins are described herein. The method of the present invention exploits the differences in electrical time constants for the media inside the cell and outside the cell to produce a net force to cause cellular lysis and extract cellular components. The method of the present invention can be used in the treatment of flocculated as well as unflocculated algal cell cultures. The device of the present invention provides efficient cell lysing in a low-energy cost set-up.

Abstract: A process and an apparatus separate a volatile component from a substance mixture (raw product). The substance mixture is heated, the volatile component is at least partly evaporated and then the vapor formed is condensed. To improve the separating action, the condensate, in the region of the point of condensation, is contacted with a liquid in which the condensate dissolves.

Abstract: A method produces fatty acid esters by transesterification of fats and oils with the aid of an alcohol, at a high pressure and temperature. Unreacted alcohol is separated inline from the reaction mixture and continuously recycled into the transesterification process. The separation is performed by obtaining a vapour phase and higher density phases of the reaction mixture and concentrating the alcohol in the vapour phase.

Abstract: The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

Abstract: Disclosed is a filter suitable for use with a deep fryer for filtering foreign particulates, such as solid particles, from cooking oil or shortening to minimize the carbonation of such particles and extend the useful life of the cooking oil. In particular, the filter does not use disposable paper filtration media.

Abstract: Method for improving the performance of a preparative batchwise Super Critical Fluid Chromatographic separation for the recovery of a target fatty acid or derivative thereof, or of a group of target fatty acids or of derivatives thereof from a mixture, characterized in that the said Super Critical Fluid Chromatographic separation employs a chromatographic column charged with a stationary phase chosen from the group consisting of aluminium oxide or aluminium oxides, titanium oxide or titanium oxides, silica gel or silica gels, amino propyl modified silica gels, diol modified silica gels, phenyl modified silica gels and “reversed phase” phases, the former in turn chosen from the group consisting of RP18, RP8 and RP3, and in that the said Super Critical Fluid Chromatographic Separation further employs a mobile phase chosen from the group consisting of ethylene, propane, ammonia, dinitrogen dioxide and CO2, wherein in the said method, during the run of the said mixture on the chromatographic column, a pressure an

Abstract: The present invention discloses a commercial process for the production of refined palm oil rich in natural carotene. The process for the production of natural carotene rich refined oil comprises the steps of: (i) fractionating crude palm oil to form a liquid fraction and a solid portion; (ii) separating the liquid fraction from the solid portion; (iii) degumming the liquid fraction; (iv) neutralizing the degummed liquid fraction to substantially remove free tatty acids and impurities; (v) washing off the soap resulting from the neutralization step; and (vi) deodorizing the neutralized the product of step (v) to remove remaining free fatty acids and other impurities contained therein, thus forming a refined palm oil that contains less than 0.1% free fatty acid, has a carotene content retained of about 75%, an iodine value of less than 60 and a peroxide value of zero.

Abstract: Methods of producing useful products, such as hydrocarbons and other molecules that are useful as fuels, from carbonaceous materials, are disclosed. Such methods include obtaining a carbonaceous material, such as coal, from a deposit and treating the carbonaceous material with one or more chemicals, including acetic acid, salts of acetic acid, esters of acetic acid, hydroxides and peroxides, alone or in combination, to solubilize the material in preparation for further processing, such as bioconversion, to produce useful products, or solubilizing the carbonaceous material in a formation using the above-recited chemicals, removing the solubilized material from the formation and bioconverting it to produce useful products, or solubilizing the material using the above-recited chemicals and bioconverting at least a portion of the solubilized material in a formation followed by recovery of useful products from the formation.

Abstract: A method for producing biofuels along with valuable food and neutraceutical products is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids along with carotenoids and chlorophylls from the algal biomass, and separation of the carotenoids and chlorophylls using adsorption or membrane diafiltration or other methods. The remaining neutral lipids are esterified with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising omega-3 fatty acids esters and remaining carotenoids.

Abstract: Methods, particles, and devices are disclosed for filtration of oil for use of the oil in the preparation of biodiesel. Disclosed particles may comprise a substantially inert porous particle with a coating comprising a polymer having amine, amino, and/or imine group(s).

Abstract: The present invention relates to a process for cooling fatty acid distillate from scrubbing section in a fats and oils refinery comprising cooling the fatty acid distillate by heat recovery in at least one heat-exchanging zone with refined oils having a temperature above about 50° C. heating the refined fats and oils to a temperature above about 70° C. The present invention relates further to a process for refining crude fats and oils, and refining plant for refining crude fats and oils.

Abstract: The present invention relates to devices and methods for selectively removing contaminants from a crude product mixture. In an embodiment the invention includes a method for removing organic acids from a crude product mixture including contacting the crude product mixture with a metal oxide substrate, wherein free organic acids in the crude product mixture bind to the metal oxide substrate, thereby removing free organic acids and forming a refined product mixture; separating the refined product mixture from the metal oxide substrate; and removing the organic acids bound to the metal oxide substrate by contacting the metal oxide substrate with an alkyl ester composition at a temperature of greater than about 100 degrees Celsius. Other embodiments are also described herein.

Abstract: Methods for producing refined rice bran oil that include caustic treatment step(s) in an amount that is less than or equal to a theoretical amount of caustic required to neutralize practically all of the free fatty acids in the rice bran oil, but avoiding addition of excess caustic. An objective is to retain a high level of oryzanol in the refined oil.

Abstract: Provided herein are oil compositions comprising an interesterified structured lipid component, the component being an interesterifcation reaction product of an amount of a medium chain triglyceride, an amount of a long chain domestic oil, and an amount of a a source of stearidonic acid. The structured lipid component can be used in edible oil compositions. The oil compositions can contain an amount of a phytosterol ester component. The oil compositions disclosed herein have good stability and also have other properties useful for an edible oil composition.

Abstract: The present invention relates to a method to extract long-chain polyunsaturated fatty acids from sources using emulsifiers dispersed in oil as carrier and to the oils obtainable from such a method. It also relates to the use of emulsifier in oil for improving the extraction of long-chain polyunsaturated fatty acids in the form of phospholipids and, in a further aspect, to an oil composition comprising long-chain polyunsaturated fatty acids in an amount up to 4% and phospholipids in an amount up to 15% and at least one emulsifier.