Abstract: The present invention relates to a method for the production of fat with a principal application as transportation biofuel or a component or raw material therefor. According to the method, cell masses, cell suspensions and/or liquid phases formed in the production of single cell oil, and/or biomass-containing side streams or microorganism cell masses for another purpose and/or originating from other sources, are contacted with a fat-production capable microorganism and the organism is allowed to produce fat. The resulting fat is recovered or the microorganism mass is passed to a single-cell oil production process. By means of the invention, the organic matter present in the cell mass and side streams of single-cell oil can be re-utilized for the production of the single-cell oil, thereby improving a total fat yield, as well as reducing an organic load of the side streams.

Abstract: Provided herein are methods, kits and compositions useful in detecting degradative enzymes and biomolecules in bodily fluid samples.

Abstract: The present invention provides a method for the production of at least one fatty acid and/or oil from a plant cell suspension culture, the method comprising (i) maintaining a cell suspension culture of oil-producing plant cells under conditions such that the cultured cells synthesise and secrete at least one fatty acid and/or oil into the cell suspension culture medium; and (ii) extracting the thus secreted at least one fatty acid and/or oil from the cell suspension culture medium.

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein, enhanced seed oil and modified oil composition. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

Abstract: A suspension may include an aqueous liquid and suspended particles. The particles may include a nonpolar and/or hydrophobic substance (e.g., a lipid) substantially contained within polar and/or hydrophilic exterior layers. A method for extracting the suspended lipids may include adding a nonpolar solvent to the suspension and disrupting the exterior layers to expose the lipids to the nonpolar solvent. In some cases, particles may also include interior hydrophilic portions (e.g., intracellular water), which may be exposed to the aqueous liquid via disruption of the exteriors. The mixture may be accelerated to segregate the mixture into first and second products. The first product may have a majority of the nonpolar and/or hydrophobic substances. The second product may have a majority of the polar substances.

Abstract: Methods and compositions for diagnosing the presence of a cancer cell in an individual are provided. Methods and compositions for identifying a tumor-specific signature in an individual having cancer are also provided. Methods and compositions for diagnosing the presence of an infectious agent in an individual and/or for identifying an infectious agent-specific signature in an infected individual are provided. Methods and compositions for diagnosing the presence of a disease in an individual are also provided. Methods and compositions for identifying a disease-specific signature in an individual having the disease are also provided.

Abstract: A process for producing a glyceride product which is enriched in monounsaturated fatty acids relative to the starting glyceride comprising the steps: (a) alcoholysis of triglycerides employing lipolytic enzymes selective for saturated fatty acids and/or lipolytic enzymes selective for the 1-position, the 3-position or both positions in a glyceride; and (b) separation of fraction A which is enriched in saturated fatty acid esters from fraction B which is enriched in monounsaturated glycerides.

Abstract: A process for purification of a liquid lipid from a bed of a particulate absorbent, where a supercritical fluid is passed through said bed to release lipids retained on the absorbent. Also apparatus for use in the performance of the process, lipids purified using the process and by-products of the process. The process enables enhanced removal of retained lipids from the particulate absorbent, e.g. removal of plant oils from bleaching clays.

Abstract: This invention relates to a lignin sorbent, a lignin removal unit, a biorefinery, a process for removing lignin, a process for binding lignin, and a renewable material. The lignin sorbent includes a substrate, and a lignin binding material dispersed with respect to the substrate. The lignin binding process includes the step of adding a lignin binding material to an input stream, and the step of converting the input stream into a renewable material.

Abstract: A method for the parallel identification of one or more metabolite species within a biological sample is provided. The method comprises producing a first spectrum by subjecting the sample to a nuclear magnetic resonance analysis, the first spectrum containing individual spectral peaks representative of the one or more metabolite species contained within the sample; producing a second spectrum by subjecting the sample to a mass spectrometry analysis, the spectrum containing individual spectral peaks representative of the one or more metabolite species contained within the sample; subjecting each of the individual spectral peaks to a statistical pattern recognition analysis to identify the one or more metabolite species contained within the sample; and identifying the one or more metabolite species contained within the sample by analyzing the individual spectral peaks of the mass and nuclear magnetic resonance spectra.

Abstract: A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.

Abstract: The invention relates to the use of esters of 2-butyloctanol with C2-36 carboxylic acids or C2-36 dicarboxylic acids in cosmetic and/or pharmaceutical preparations. The compounds are characterised by a particularly light feeling.

Abstract: The invention relates to the use of esters of 2-ethylbutanol with C4-36 carboxylic acids or C4-36 dicarboxylic acids in cosmetics and/or pharmaceutical preparations. The compounds are characterised by a particularly light feeling.

Abstract: This invention relates to an enzyme recovery sorbent, an enzyme recovery unit, a lignocellulosic biorefinery, a process for recycling enzymes, and a renewable material. The invention includes a lytic enzyme recovery sorbent suitable for use in production of renewable materials. The sorbent includes a substrate, and an enzyme binding material dispersed with respect to the substrate.

Abstract: The subject application provides an aqueous composition suitable for use as an artificial tear solution comprising one or more lipids produced by an enzyme of the diacylglycerol acyltransferase 2 (DGAT2) family and/or the acyl-CoA cholesterol acytransferase (ACAT) family. This invention also provides related methods.

Abstract: Methods of producing fatty acid esters, such as fatty acid ethyl esters, from genetically engineered microorganisms are described.

Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, are described.

Abstract: The invention relates to methods for producing secondary metabolites using transformed Synechocystis sp. bacteria, and secondary metabolites produced by transformed Synechocystis sp. bacteria. The invention further relates to phosphopantetheinyl transferase enzymes.

Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.

Abstract: The methods, kits, articles, and compositions of the invention feature a natural product (e.g., Sargassum fusiforme), an extract thereof, and fatty acid components of the extract (e.g., palmitic acid) for the treatment of a viral infection, e.g., HIV or herpes. The natural products used in the methods and compositions of the invention include brown algae, specifically algae of the Sargassum fusiforme species. The Sargassum fusiforme algae, extracts thereof, specific components of the extract, and related compounds of the invention may be used to treat or prevent HIV and herpes infections.

Abstract: Provided herein are systems and methods for producing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and/or derivatives and/or mixtures thereof by growing algae that produce the oils containing EPA and/or DHA and/or derivatives and/or mixtures thereof, harvesting the algae with fish in one or more enclosed systems, and then processing fish to separate and purify the EPA and/or DHA. The multi-trophic systems provided herein comprise at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. Also provided herein are the lipid compositions extracted from the fishes.

Abstract: The present invention provides lipid-based formulations for delivering, e.g., introducing, nucleic acid-lipid particles comprising an interference RNA molecule to a cell, and assays for optimizing the delivery efficiency of such lipid-based formulations.

Abstract: The present invention is directed toward fatty acid-based particles, and methods of making such particles. The particles can be associated with an additional, therapeutic agent. Also provided herein is a method of forming fatty acid particles, comprising associating a cross-linked, fatty acid-derived biomaterial with a cryogenic liquid; and fragmenting the bio material/cryogenic liquid composition, such that fatty acid particles are formed. The particles can be used for a variety of therapeutic applications.

Abstract: A method of fractionating biomass, by permeability conditioning biomass suspended in a pH adjusted solution of at least one water-based polar solvent to form a conditioned biomass, intimately contacting the pH adjusted solution with at least one non-polar solvent, partitioning to obtain an non-polar solvent solution and a polar biomass solution, and recovering cell and cell derived products from the non-polar solvent solution and polar biomass solution. Products recovered from the above method. A method of operating a renewable and sustainable plant for growing and processing algae.

Abstract: The invention relates to esters of 2-propylheptanoic acid and also to the use of esters of 2-propylheptanoic acid in cosmetic and/or pharmaceutical preparations. The compounds are characterized by the particularly lightweight sensation thereof.

Abstract: The present invention provides novel lysophosphatidic acid acyltransferase genes. A nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 1, 3, 36 or 37 or a fragment thereof.

Abstract: Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.

Abstract: The present invention provides a new additional identification of a gene related to cancer expression and a diagnostic kit using the same.

Abstract: A shelf-stable consumable composition may comprise one or more Probiotic-Mimicking Elements (PMEs) wherein the median particle size of the one or more PMEs is less than the median particle size of a probiotic from which the one or more PMEs were derived. In some embodiments, the median particle size of the one or more PMEs may be less than about 0.2 microns. In some embodiments, the PMEs may comprise at least one of peptides, fatty acids, bacteriocins, cell surface proteins, phospholipids, teichoic acids, and nucleic acids, and may be combined with one or more prebiotics, such as oligosaccharides, inulin, short-chain fructo-oligosaccharides, xylooligosaccharides, galactooligosaccharides, soyoligosaccharides, and lactulose/lactitol. Methods for preparing and using a shelf-stable consumable composition for promoting digestive health in a subject are also described.

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-9 elongases in plants.

Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

Abstract: It is intended to provide a procedure for, in the fractionation of vegetable butter, transesterified fat or oil, isomerized hydrogenated fat or oil, etc. without the use of solvents, obtaining high-concentration component G2U (defined below) by concentrating operation through reduction of the amount of liquid component residue in crystal portion. There is provided a method of dry fractionation of fat or oil characterized in that fat or oil (A) containing components G2U and GU2 is fractionated through crystallization/solid-liquid separation into crystal fraction of concentrated G2U (AF) and liquid fraction of concentrated GU2 (AL), subsequently this crystal fraction (AF) is mixed with liquid G2U-containing fat or oil (B) whose GU2 concentration is lower than that of the liquid fraction (AL) and thereafter the mixture is separated into crystal fraction (BF) and liquid fraction (BL).

Abstract: The use of a biologically active complex of ?-lactalbumin, selected from HAMLET (human ?-lactalbumin made lethal to tumour cells) or a biologically active modification thereof, or a biologically active fragment of either of these, in the preparation of a medicament for use in the treatment of papilloma, such as cutaneous papillomas.

Abstract: A method for producing starting materials or additives for cosmetics, pharmaceuticals and/or hydrocarbon-based fuels, especially for heating systems or internal combustion engines, takes organic residues or waste materials and decontaminates or sterilizes them with a hydrogenation process. In particular, the materials classified as K1, K2 or K3 according to EU Directive 1774/2002 are used for generating or producing the cited starting materials or additives, whereby an adequate decontamination of the residues or waste materials that are classified as being hazardous to health is ensured.

Abstract: The present invention relates to methods of improving the low temperature storage and performance properties of fatty acids and/or derivatives thereof, as well as compositions containing fatty acids and/or derivatives thereof having superior lower temperature storage and performance properties.

Abstract: A new and distinct variety of loblolly pine tree named ‘01PM0212’, particularly characterized by uniform rapid growth; good stem straightness; moderate rust resistance; and moderate to long internode.

Abstract: The present invention provides a transgenic soybean event MON87705, and cells, seeds, and plants comprising DNA diagnostic for the soybean event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for said soybean event in a sample, methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event in a sample, growing the seeds of such soybean event into soybean plants, and breeding to produce soybean plants comprising DNA diagnostic for the soybean event.

Abstract: The invention provides systems and methods for producing biofuel from algae that use cultured fish to harvest algae from an algal culture. The methods further comprise gathering the fish, extracting lipids from the fish, and processing the lipids to form biofuel. The multi-trophic systems of the invention comprises at least one enclosure that contains the algae and the fishes, and means for controllably feeding the algae to the fishes. The lipid compositions extracted from the fishes are also encompassed.

Abstract: The present disclosure provides contrast photoacoustic probes, and compositions comprising such probes, designed to non-invasively detect and monitor various disease states, or targets within a subject human or animal. The probes are designed to be optically excited in tissue, ultimately generating thermal energy, which is transformed into acoustic energy by the response of the aqueous environment in the subject to the thermal emissions. The acoustic energy (sound) can then be detected by suitably applied transducers and digitally transformed into images indicating the location of the probe in the subject. One aspect of the disclosure encompasses photoacoustic probes that comprise: a carbon nanotube and a plurality of dye molecules bound to the carbon nanotube. The probes may further comprise a targeting moiety for localizing the probe at the site of a specific target.

Abstract: Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 ?-subunit, Gal83 ?-subunit or Snf4 ?-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

Abstract: Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant roculture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass.

Abstract: There are disclosed preparations of plant oils which may be highly pure, have a low moisture content and may be highly stable. Methods and apparatus for the preparation of the oils may exclude electromagnetic radiation and reactive gasses during the preparation of the oils.

Abstract: The present invention discloses antimicrobial compositions of modified coconut oil and palm kernel oil derived from catalytic activity of 1,3 positional specific lipases. Said modified oil compositions comprises of free fatty acids (>9.4%), monoglycerides (>1.3%), diglycerides (>22.8%) and triglycerides (>25%) which inhibits the growth of gram positive bacteria i.e. Staphylococcus aurous aureus, Listeria monocytogenes, Sterptococcus pyogene, gram negative bacteria i.e. Vibrio cholerae, Escherichia coli and yeast i.e. Candida albicans.

Abstract: A reactor comprising at least one contact surface made from, coated with, or impregnated by a catalyst, wherein the contact surface comprises a sintered metal or a ceramic, and wherein the reactor is configured to subject a reactant stream to shear. A system for carrying out a heterogeneously catalyzed reaction, the system comprising a reactor as described above and a pump configured for delivering reactants to the at least one reactor. A method for carrying out a heterogeneously-catalyzed reaction by introducing reactants into a reactor comprising at least one contact surface made from, coated with, or impregnated by a catalyst under conditions which promote production of a desired product, wherein the contact surface comprises a sintered metal or a ceramic, and forming a dispersion of reactants within the reactor, wherein the dispersion comprises droplets or gas bubbles of reactant with an average diameter of less than about 5 ?m.

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding novel delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

Abstract: Recombinant photosynthetic microorganisms that convert inorganic carbon to secreted fatty acids are described. Methods to recover the secreted fatty acids from the culture medium without the need for cell harvesting are also described.

Abstract: An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. Both the heating and the cooling stages are rapid, with the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least ?0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, ° C.) graph, one obtains a trapezium having an area less than 13,000° C. minute. Not only does this result in a smaller energy input (and so a reduction in costs), but a better quality (and less oxidised) oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

Abstract: The present invention relates to plants, seeds and products derived thereof in particular to Brassica plants, seeds products derived thereof, that have mutant sequences conferring high oleic acid profile on the seed oil. More particularly, the invention relates to mutant delta-12 fatty acid desaturase sequences, also referred to herein as FAD2 sequences, in such plants which confer high oleic acid profile on the seed oil.

Abstract: The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “?5-desaturase”) and at carbon 6 (i.e., “?6-desaturase”) and to uses thereof. In particular, ?5-desaturase may be utilized, for example, in the conversion of dihomo-?-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to ?-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.

Abstract: The present invention relates to a transport agent for transporting nucleic acids into eukaryotic cells and a method for producing said agent. The invention further concerns methods for transporting nucleic acids into eukaryotic cells using the transport agent according to the invention. The present invention provides an alternative transport agent and a method which are effective to allow an efficient transport of nucleic acids into eukaryotic cells. The transport agent comprises a complex forming moiety that is capable of forming complexes with at least one nucleic acid molecule and condensing said nucleic acid molecule, and at least one nuclear localization moiety comprising at least one nuclear localization signal and having an approximately neutral net charge.