Abstract: The present invention relates to a purification and concentration method for proteins and antibodies. Particularly the present invention relates to a continuous surfactant based phase separation method for recovering hydrophobin fusion proteins, and for recovering target molecules, such as antibodies, directly from a liquid by using phase separation and hydrophobin-Protein A fusion technologies.

Abstract: A trimodal hybrid particle mix loss prevention material (LPM) is provided. The trimodal hybrid particle mix LPM includes date palm seed particles produced from date palm seeds, scrap tire particles produced from scrap tires, and date tree pruning waste particles produced from date tree pruning waste. The LCM may include date palm seed particles in the range of about 40 wt % to about 50%, scrap tire particles in the range of about 30 wt % to about 40 wt %, and date tree pruning waste particles in the range of about 15 wt % to about 25 wt %. Methods of loss prevention and manufacture of a trimodal hybrid particle mix LPM are also provided.

Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.

Abstract: A fermenter and a method of fermentation in a U-shape and/or nozzle U-loop fermenter (100) comprising a U-part having an essentially vertical down-flow part (101), an essentially vertical up-flow part (102) and a substantially horizontal connecting part (103), which connects the lower ends of the down-flow part (101) and the up-flow part (102), a top part (104) which is provided above the U-part and has a diameter which is substantially larger than the diameter of the U-part, means for creating liquid circulation in U-part of the fermenter, and one or more gas injection points (110) for the introduction and dispersion of the gas(ses) into the fermentation liquid. The pressure may be controlled differently in certain zones of the fermenter by pressure controlling devices (105, 106, 108) e.g.

Abstract: The application provides, among others, methods for constructing animal obesity models, methods for screening microorganism or composition of microorganisms that may cause obesity, methods for screening therapeutic targets for treating metabolic disorders, and methods for screening or evaluating microorganisms, compounds, food, recipes, formulations, drugs, nutritional supplements, healthcare products, beverages and other items for preventing and treating metabolic diseases.

Abstract: A novel soybean variety, designated 5PLET90 is provided. Also provided are the seeds of soybean variety 5PLET90, cells from soybean variety 5PLET90, plants of soybean 5PLET90, and plant parts of soybean variety 5PLET90. Methods provided include producing a soybean plant by crossing soybean variety 5PLET90 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PLET90, methods for producing other soybean varieties or plant parts derived from soybean variety 5PLET90, and methods of characterizing soybean variety 5PLET90. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PLET90 are further provided.

Abstract: Provided is a modified xylanase. The polypeptide has xylanase activity and has an amino acid sequence with deletion of at least 12 consecutive amino acid residues at the C-terminus of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90% identity with the amino acid sequence of SEQ ID NO: 1.

Abstract: Furfural is produced from biomass material containing pentosan, in high yields, in a production process comprising treating the biomass with a solution containing at least one ?-hydroxysulfonic acid thereby hydrolyzing the biomass to produce a product stream containing at least one C5-carbohydrate compound in monomeric and/or oligomeric form, and dehydrating the C5-carbohydrate compound in the presence of an acid, in a biphasic reaction medium comprising an aqueous phase and a water-immiscible organic phase, at a temperature in the range of from about 100° C. to about 250° C. to produce a dehydration product stream containing furfural. An aqueous stream is separated from the dehydration product that can be optionally recycled to the hydrolysis step.

Abstract: A method for cell-free protein synthesis is characterized in that pH is controlled by using an enzyme. For example, by using an amino acid decarboxylase, the pH is controlled according to removal of hydrogen ions that are produced during regeneration of ATP. The method for cell-free protein synthesis of the present invention has an advantage that not only the expression amount of protein is enhanced but also the expressed protein can be directly used for activity analysis without undergoing any separation or purification.

Abstract: A novel soybean variety, designated 5PGBK62 is provided. Also provided are the seeds of soybean variety 5PGBK62, cells from soybean variety 5PGBK62, plants of soybean 5PGBK62, and plant parts of soybean variety 5PGBK62. Methods provided include producing a soybean plant by crossing soybean variety 5PGBK62 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PGBK62, methods for producing other soybean varieties or plant parts derived from soybean variety 5PGBK62, and methods of characterizing soybean variety 5PGBK62. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PGBK62 are further provided.

Abstract: Genetically manipulated cells, lysates of such cells, systems, and methods of use thereof are provided, where one or more enzymes in a pathway of interest are genetically modified to incorporate a peptide sequence that provides for relocation of the protein, e.g., to the periplasm, so as to sequester the enzyme, and where the enzyme controls flux in the pathway of interest.

Abstract: The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g.

Abstract: The present invention relates to polypeptides having cellulolytic enhancing activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.

Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol.

Abstract: A method of isolation of polyhydroxyalkanoates from biomass pre-cleans the biomass in a first extraction process by extracting components of the biomass other than polyhydroxyalkanoates. In a second extraction process, polyhydroxyalkanoates are extracted from a solid phase of the biomass obtained in the first extraction process by adding an extraction agent to pre-cleaned biomass based on chlorinated hydrocarbon, carrying out the extraction for 5 to 90 minutes at a temperature in the range of 20 to 120° C., separating the extract containing the polyhydroxyalkanoates from the extraction solution, and feeding the extract to a circulation loop filled with water having a temperature from 20 to 120° C. or a mixture of water and from up to 20% by weight of the extraction agent based on chlorinated hydrocarbon used for the extraction of polyhydroxyalkanoates, wherein the extraction agent is removed from this extract and polyhydroxyalkanoates precipitate.

Abstract: A method of producing a sugar liquid from a cellulose-containing biomass includes (1) hydrolyzing a cellulose-containing biomass using a filamentous fungus-derived cellulose and carrying out solid-liquid separation into a sugar liquid and a hydrolysis residue; (2) washing the hydrolysis residue in (1) using an aqueous alkaline solution and an aqueous inorganic salt solution independently and recovering, as a washing liquid, the filamentous fungus-derived cellulase adsorbed to the hydrolysis residue; and (3) filtering the washing liquid in (2) through an ultrafiltration membrane to recover a sugar liquid as a permeate and the filamentous fungus-derived cellulase as a non-permeate.

Abstract: The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

Abstract: Present invention enables to mass-produce rich-taste tamari soy sauce with reasonable costs. First step: Moisturizing soybeans. Second step: Steaming the moisturized soybeans. Third step: Shaping the steamed soybeans into nuggets. Fourth step: Mixing a soy flour and seed koji. Fifth step: Putting the mixture on the nuggets. Sixth step: Incubating the nuggets. Seventh step: Putting the incubated nuggets in salt water. Eighth step: Fermenting the nuggets in the salt water. Ninth step: Pressing moromi (solid component of the fermented product). Tenth step: Settling the liquid obtained by pressing the moromoi. Eleventh step: Optionally filtrating raw tamari soy sauce with a reverse osmosis membrane. Twelfth step: Adjusting concentrations of some constituents of the raw tamari soy sauce. Thirteenth step: Pasteurizing the raw tamari soy sauce. Fourteenth step: Settling the pasteurized tamari soy sauce. Fifteenth step: Filtering the supernatant. Sixteenth step: Bottling the tamari soy sauce.

Abstract: A system and method for producing bio-organic compounds may include a vessel, a first phase comprising an aqueous medium including host cells capable of producing a bio-organic compound, where the bio-organic compound comprises a second phase in contact with the aqueous medium.

Abstract: A process for treating contaminated water has the steps of filtering the contaminated water through a filter so as to produce a filtrate therefrom, introducing nutrients and a biomass into an interior volume of the of a light reactor, passing the filtrate into the light reactor, reacting light with the nutrients and the biomass so as to produce a light-reacted biomass, transferring the light-reacted biomass to a dark reactor, reacting the transferred light-reacted biomass with carbon dioxide in the dark reactor, and filtering the reacted biomass from the dark reactor so as to remove the biomass therefrom. The nutrients in the biomass are continuously stirred within the light reactor in a toroidal circulation pattern.

Abstract: Methods for diagnosing a systemic or autoimmune disorder and methods for treating the same by inducing the production of or otherwise providing an autoantibody that recognizes a skin basement membrane component.

Abstract: Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

Abstract: A culture vessel and a method of growing cells in a culture vessel. The culture vessel includes a container and a plurality of agitators. The container receives a medium and cells. The plurality of agitators is positioned to contact the medium when received in the container and configured to promote suspension of the cells in the medium upon oscillation of the agitators. The agitators are at least one of coupled to and formed integrally with the container such that there is no relative movement therebetween. The method includes receiving a medium and cells in a container and oscillating the medium such that the cells traverse along a longitudinal direction of the container.

Abstract: Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels.

Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.

Abstract: The present invention relates to a novel liquid preparation for the topical treatment for itching and inflammation associated with psoriasis and other common skin maladies wherein a layered mass of organic and inorganic material comprising: 1) Fruits, vegetable by-products, and paper scraps; 2) Grit comprising a blend of crushed volcanic rock; and, 3) A microbial soil enhancer is composted by a population of selected earthworms. The composting mass is misted daily to achieve a uniformly moist consistency. For the first two weeks, any worm tea (runoff) exiting the composting mass after each daily misting is recycled into the next day's misting fluid. A fraction of the worm tea produced during the third through twelfth weeks is harvested, pH adjusted, and treated with surfactant to form the liquid preparation.

Abstract: A novel soybean variety, designated XBP05005R is provided. Also provided are the seeds of soybean variety XBP05005R, cells from soybean variety XBP05005R, plants of soybean XBP05005R, and plant parts of soybean variety XBP05005R. Methods provided include producing a soybean plant by crossing soybean variety XBP05005R with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XBP05005R, methods for producing other soybean varieties or plant parts derived from soybean variety XBP05005R, and methods of characterizing soybean variety XBP05005R. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XBP05005R are further provided.

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A method of manufacturing coumaramide includes treating cellulose-based biomass with an ammonia-containing treatment agent to obtain ammonia-treated biomass, immersing the ammonia-treated biomass into a solvent containing at least water and eluting coumaramide in the ammonia-treated biomass into the solvent to obtain a coumaramide solution, and precipitating coumaramide in the coumaramide solution as a crystal to obtain a coumaramide crystal.

Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.

Abstract: The present invention relates to extracts of Tetraselmis sp., preferably Tetraselmis suecica, its cosmetic, dermatological and/or therapeutic uses and compositions and cosmetic, dermatological or therapeutic products comprising such an extract of Tetraselmis sp., preferably Tetraselmis suecica.

Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Abstract: The present invention relates to maintaining the activity and stability of enzymes and biologically active proteins at increased temperatures by contacting same with a combination of isolated passive and active chaperones from a hyperthermopilic Archaeon, wherein the chaperones may include heat shock proteins, prefoldin and/or chaperonin proteins.

Abstract: This invention discloses a constitutive promoter for expression of foreign or endogenous coding sequences in plants, including dicotyledonous and monocotyledonous plants. The invention also discloses a chimeric nucleic acid construct comprising the promoter of the invention operably linked to a foreign or endogenous polynucleotide that codes for a protein of interest or a transcript capable of modulating expression of a target gene. The invention further discloses transformed plant cells, as well as differentiated plants and plant parts, containing the construct. Methods for diagnosis and treatment of viral infections, especially badnaviral infections, are also disclosed.

Abstract: The invention relates to the soybean variety designated XB36G13. Provided by the invention are the seeds, plants and derivatives of the soybean variety XB36G13. Also provided by the invention are tissue cultures of the soybean variety XB36G13 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety XB36G13 with itself or another soybean variety and plants produced by such methods.

Abstract: Disclosed is a general methodology to create nanofibers of therapeutic molecules that have a dual role, as both the delivery vehicle and the drug itself. It is shown that with proper molecular design, the integration of enzymatic reaction and self-assembly provides a powerful method to create molecular hydrogels of clinically-used therapeutics without compromising their bioactivities. In addition, the results disclosed herein demonstrate enzyme-instructed self-assembly as a facile strategy for generating the supramolecular hydrogels of molecules that inherently have poor solubility in water. For example, by covalently connecting paclitaxel with a motif that is prone to self-assemble, a hydrogel of paclitaxel can be formed without compromising the activity of the paclitaxel.

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention discloses a compound separated from Monascus-fermented rice, the preparation method and uses thereof. Specifically, the present invention discloses a compound represented by the Formula I, or a pharmaceutically acceptable salt thereof, wherein, R1 is selected from the group consisting of hydrogen, hydroxyl, C1-6 straight or branched alkoxyl, (II), (III) and (IV); R2 is hydrogen or C1-6 alkyl; R3 is selected from the group consisting of hydrogen, hydroxyl and C1-6 straight or branched alkoxyl. The present invention further discloses a pharmaceutical composition containing the compound. The compound of the present invention has HMG-CoA reductase inhibition effects.

Abstract: The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces of gravity, fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.

Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The invention relates to a polypeptide which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 76% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Abstract: A one-piece solid phase extraction article to be used in solid phase extraction analysis, a filtration funnel comprising the one piece solid phase extraction article, and a method of use of the article. The article, device and method provide for improved analysis of hexane extractable material by eliminating the standard use of polar solvents to pre-condition the sorbent materials. The one-piece solid phase extraction article comprises water wettable monomeric phase type sorptive material embedded within a porous matrix. The water wettable monolithic polymeric sorptive material may be a hydrophilic polymeric gel, such as functionalized C-18 sorbent material, and the porous matrix may be fibrous matric, such as a glass fiber. The one-piece solid phase extraction article may further comprise one or more pre-filtration layers which do not contain the sorptive particles.

Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

Abstract: This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions, e.g., consortia, that are both specifically optimized to stimulate methanogenesis, or for “methylotrophic” or other conversions. In alternative embodiments, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In other embodiments, the invention provides methods for simulating biogas in sub-surface conditions using a computational model.

Abstract: Provided is a pretreated product for saccharification, obtained by a pretreatment alone which allows an efficient enzymatic saccharification treatment to thereby obtain a saccharified solution having a uniform quality. A pretreated product for saccharification where lignin 5 is dissociated or a substrate 1 is swollen is obtained by pretreating the substrate 1. The substrate 1 has 60 to 80% of the pores having a diameter of 10 to 50 nm. In a process for producing the pretreated product for saccharification, a substrate mixture obtained by mixing the substrate 1 and ammonia water is maintained at a predetermined temperature for a predetermined period of time to thereby separate a part of hemicellulose 4 having a molecular weight of 7.8×103 to 2.0×106.

Abstract: A method of producing a compound originating from a polysaccharide-based biomass includes at least one of a saccharification step that produces a sugar solution containing a monosaccharide and/or an oligosaccharide from a product obtainable by hydrolyzing the polysaccharide-based biomass; a fermentation step that ferments the sugar solution containing the monosaccharide and/or oligosaccharide originating from the polysaccharide-based biomass; and a treatment that removes a fermentation inhibitor with the use of a separation membrane having a glucose removal rate and an isopropyl alcohol removal rate which simultaneously satisfy the following relationships (I) and (II) when a 500 ppm aqueous glucose solution at pH 6.5 at 25° C. and a 500 ppm aqueous isopropyl alcohol solution at pH 6.5 at 25° C. are respectively permeated through the membrane at an operation pressure of 0.

Abstract: Provided herein are novel ACCases and nucleotides encoding the same, that when introduced into a cell or organism results in an increase and/or accumulation of fatty acids, glycerol lipids, and/or oils in the cell or organism, and/or a change in the types of fatty acids, glycerol lipids, and/or oils that are normally present in the cell or organism. Also provided herein are organisms transformed with the novel ACCases.